CmaCh07G003320 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G003320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
LocationCma_Chr07: 1445967 .. 1451025 (-)
RNA-Seq ExpressionCmaCh07G003320
SyntenyCmaCh07G003320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAAGTTCTTTCGTCTCCAATGGTGAAGCCGCCGTCAAATCTTCTCCTGCGGCGGTGTCCGGACGGGTCTCCTCAGTGTATGCTGAAGTTCAGTCCAGTCGTATCGACCACACTCTGCCTCTGCCTTCTGTGCTCAGAAGTTCCTTCACGATCGTCGATGGACCCCCTAGCTCTGCCGCTGGCAATCCAGGTACTCTGTTCTATTGCATGCTCTCGGGATCTGTGTATGTATCACTTTTGTTTCATCTTGTAGTGTTTATTATTACTTTTGATCCTTGAAGAATCTGGTTGCTGCTTGCTGAATCAGGAAATGAGTTTTTAGCAATTTTAAGTGTCTTTTCGATTTTATTCGATCGAATGGGAACATTAAAAATGGAAGCTGTTGCTTACGCTTCAGAGTTTTCTGTTCACTCAATACATATTTTGGTGTCTGTTTCTGATTTCATAGACTTCCATGAATTTTTGTGATGCATGAGCCTTGTATCTCCTTCTATTATTCCTGTGCATGGCTTTGGAAGGAGGACTGCAATTTAGGCGTTTTATTAATCTTCTTTGCGTTTGTTTTAAGCAGACGAGATAGCCAAGCTATTTCCTAATCTTTTCGGCCAGCCATCGGCAAAATTGGTACAAAGCAATTCCAACAAAGCTCAACCGGACAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAACGTGATTTCCGGAATTTTTGGTAACGATTATTTTACATTTACTGAATTAAATCTGATAAATCTATTCTAATCTACTTCCGAATCATGTTTCTTTTATTATTTGGATTTGAGTGATTTTAAATCTGTGTTGGCGATAGATTATTTGCAGGATCATGTCAAAGGCAGTGTGTTATATGGCTTCAAGGGTGGTCCTGCCGGGATTATGAAATGTAATTATGTGGAATTGAATTCCGAATATATTCATCCATATAGAAATCAGGTAAACCTAGAGTGCTTCACAACTTTAAACATTTAAATACTAGTAACGATGTTGACCTCTAATGGATTTCGATACTCTATATTAATGCTTGGTCATCATATCACTATGTAAAATAAGTTAACTGTTGTATGAGCATAATTGATGCTGGTTATTGAGACCTTTTCAGGGTGGATTTGATATGATATGCAGTGGGAGGGACAAATTTGAAACAACAGAGCAGGTGAGACTGTTTCTTTTCAACGGTCAGACATTATGCTGAAGTGACTTGGATATATGAACTTAGTTGAGTGGGCTTAATTTTTCAGTTCAAACAAGCTGAAGAAACTGCAAAAAAGCTTGATTTGGATGGGATTGTTGTGATTGGTGGAGATGATTCCAATACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGTGGACTGCTCTATTTAAGAAAATTTGCTTTTCCGTACATTGTTTTAGTTGTATCTATCATATGTTTTGTGAAATAATACCTCTTCTTTTGATCAGGAGTAAAAATTTGAAGACCCGGGTTATTGGTTGCCCCAAAACAATTGATGGAGATCTAAAATGCAAAGAGGTGCCCATAAGTTTTGGATTTGATACAGCTTGCAGGGTAACTAACCATTTCTCTCGTTGAATTCTTAACATAGTGTTAATTCTTTTGATGTAAACTACACATGTTTGCCCTATGATTGTTTGAATGCTTTTTTCAATTTTGTTCTTGTTGTGGTAGTAGATATTACCATCTCGAGTTTTTGCTCAAGTGTTAACACATCTCCTTGAACTTGTGATTTCTTACGAAGTAGAAGGCTTTAGACTTGTTATTACTTTGCAGAGAGAATAAAGTTGTCTGGGTTTTACAGTTTTACTAGATTGGATTTGCACTACGTTGTTCATTTGCCACTATTAAAGAAGTTCATGTTTCTAATGTGGCAGATTTATTCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTGTAAGTATATCAATACTCAAATTAAGAACCATAGTGTTGTCATTCAATCATATGTTGTGTGGCCCGATGGAAATCCATTTGCTACTGTCTGGAGTGGTGGTTCACACAGTTTATAATTGGTACCTTATGGATTAAAATAATCATCAATAATACCGCCAGTATGATTCTGATATTTGATAATAGTTTCTATAGTTCTTTGAAAACATTAAGAAAGTCGTATATCTTTACCTAACAGATTAAAATGTTCTTTTTTTTTTAAAAATCTATTCATAATGCTTATTTACGTTGTGGATACATTTATTTATCAGCTAATAAACTGTTTGATGCCTCAAGAAAGAGGCATTTATGAGTTGTTATGTTAGTACATAGTTCTAAATAAGCGCTTTAGAATGCATGTGGATCATTGATTTGTTTTTGCGTTGTCGTATTTCTCTTAGCATATTATTTGATTTGTGAATCTGCAGTTGTGAGGCTTATGGGACGTGCTGCTTCACACATCACCCTAGAGTGTGCTCTGCAGACTCATCCAAATATTACTATTATTGGGGAAGAGGTACTATTAACTTCTTGTCAGCTACATTTTTAATTATGTTTTTTTTTTTTTTTAATTTGTGGAAAAAGGAACAAGATTTTTATCTGTGGTGTCCTCGCATTCTACTCTCGCTCATTGCAAGTGATTCTAAATGCTCGACTTGTGTCATCTTTTATGAATGGACCTTCTTAAAACTTTATGATTGATTGGGAATCTCTGGTCGATGGTTATAAATTTTGCTGCATGTAGAGGCTACAGTGTTCCTTACTACTAGGATTGATTTGCTGCAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTCACAGATTACATTGTAGATGTAGTCTGCAAACGTGCAGAACTTGGTTTTAATTATGGTATGATACTTATTCCGGAAGGCCTTATTGACTTCATCCCGGAGGTATTATTCTTGTTCATAAAATCGGCAATGGCTGTCTTATTATCTTCATTCTTAGCTGTGTGTTTAATGAAACTATATGTTGCTCTTGAATGCCTTTCAGGTACAGCAACTTATTGCAGAGCTAAATGAAATTCTGGCCCATGAAATAGTTGATGATGAAGGATTGTGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGCTCTTGCCTCGAGCAATCCAAGAACAATTGATGCTTGAGAGAGATCCACATGGAAATGTTCAGGTAATTTTTTTTCCCTTCTGTTTTTGTTTTGATACCCAGGTGCTGGTGTTTCTTGGTTAGATATGATATTTTTCTTCTTTTCTTGTTTAGGTAGCTAAAATAGAAACAGAGAAAATGCTCATTCAGATGGTTGAAACAGAGTTGGAGAAGAGGAAGAGTGAAGGTGCATACAATGGTCAATTTAAAGGGCAATCTCACTTTTTTGGGTAAGTATTCTTCTCATTATTTGAGAACTGCAGTTCACTTTCTTTTTCTCTTTCTTCTCCTTTTGTTGGAGTTCCACAGCAACACTTGTGCGTCAAACTTGCAGATATGAAGGGAGATGTGGTCTACCAACCAATTTCGATTCTACTTACTGCTATGCATTGGGTTATGGTGCTGGATCTCTCCTGCAGAGTGGAAAAACTGGACTAATATCATCTGTCTGTCCTATTCTCTGCTCATTTTATCTTCAATATCTCTGCAAACACTTCCATCAAATATCAGTTTCCTGTTATTCCTAATTTTTTTGGCTGTGTTTGTCAATAATTTGTCTGTAAATATTTGTGCATACTCCGTGTATCTGCCAGGTGGGGAACTTGGCTGCTCCCGTTGAAGAATGGACTGTTGGTGGGACTTCATTGACATCATTAATGGATGTGGAGAGGAGACATGGTAGACTACAACTTGCTATCTTAATCACTCTGTTATCTCTATCATGCATGGTCTTAATTTTAAGTCTTGTCTTGTGGTTTCTTTCAACCCCCTTCGATTGCATGCTCCCCAAAGTGGTTTGTCCATTTTCATTAAGAATGTGTGAAAATTCTAAGGTTCAGAATTAGCTCTTTCTTTAGATCTCAAGGATAGAGTTGTACAACCCTTGGAACAGGTCCTGTTCAAGGTCGTAGAAATGACCCTTTCTTGCAAGTCATCTTTAAGAAAGGTGCTACCAAATGTATACCCGTACATTACAGGTCAAACATAAACAAAATACTTCTGGGTCGGTGTGTCTGCTTGGCATAAATTTAAACGAAAGTCCAGCATCTATTGTTCTGGAGCTTTCAACTTCTAATAACATCAATGATACAAAAGGCCCTATTTCAATAATTAGTTTCTTTTTGGTTCATGATAACTTCATTATTATGGGACGCGGCTTAGCCCCCCGAGGGTTCTTGAATCCTAGTCCAAATGTATTTAGCTGTTGCGTTCAAATGATTTGATTTTCATATACTTGTGTTATCACTGAAGTTGTATTTTTGTGACAGGCAAATTTAAGCCTGTGATTAAGAAGGCAATGGTCGAGATTGATGGTAAGAACTGTGAACGGAAGCTTTTTTTTTTTTTATTATTATTATTATTTTTTTTTTTTTTGTGTGTGTGTGTGTGTGTAATACTAGAATAGTGTCTTCCTGTTGTCATACTTACCAATCACTTTCATGTACTTATCTGCAACTGGAAAAAACTAAGCAATAATGTCCGATTCATTCATGAATTATCAGGTACCCCTTTCAAGAAGTATGCATCATTGAGGGATGAGTGGGCATTCAACAACCGATACATTAGTCCTGGTTAGAAAACATTCAACTTCTATCTTTCTCTGATTCCTTGTCCGCTCTTAACAAAAAGAGAACAATCTCCTTGTCATGTTTTTCAGCTGGCTGCCATTTAATTTATTTCCCCCTTTGCATTTACTAATATTTAAATGAGGGTGTTAAAAAAAACATGTCAATAATGCCTTAAAGATGAGAAAAGCATGCACTGTTTCGATTAAATTGTGACATTCTTCCTAATTGAAATTTCAGGTCCTATTCAATTTGTGGGTCCTGCATCAAATGCTATTAATCATACTCTCCTCCTGGAGCTTGGAGCTGGAGCTTAA

mRNA sequence

ATGCCAAGTTCTTTCGTCTCCAATGGTGAAGCCGCCGTCAAATCTTCTCCTGCGGCGGTGTCCGGACGGGTCTCCTCAGTGTATGCTGAAGTTCAGTCCAGTCGTATCGACCACACTCTGCCTCTGCCTTCTGTGCTCAGAAGTTCCTTCACGATCGTCGATGGACCCCCTAGCTCTGCCGCTGGCAATCCAGACGAGATAGCCAAGCTATTTCCTAATCTTTTCGGCCAGCCATCGGCAAAATTGGTACAAAGCAATTCCAACAAAGCTCAACCGGACAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAACGTGATTTCCGGAATTTTTGATTATTTGCAGGATCATGTCAAAGGCAGTGTGTTATATGGCTTCAAGGGTGGTCCTGCCGGGATTATGAAATGTAATTATGTGGAATTGAATTCCGAATATATTCATCCATATAGAAATCAGGGTGGATTTGATATGATATGCAGTGGGAGGGACAAATTTGAAACAACAGAGCAGTTCAAACAAGCTGAAGAAACTGCAAAAAAGCTTGATTTGGATGGGATTGTTGTGATTGGTGGAGATGATTCCAATACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGAGTAAAAATTTGAAGACCCGGGTTATTGGTTGCCCCAAAACAATTGATGGAGATCTAAAATGCAAAGAGGTGCCCATAAGTTTTGGATTTGATACAGCTTGCAGGATTTATTCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTTTGTGAGGCTTATGGGACGTGCTGCTTCACACATCACCCTAGAGTGTGCTCTGCAGACTCATCCAAATATTACTATTATTGGGGAAGAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTCACAGATTACATTGTAGATGTAGTCTGCAAACGTGCAGAACTTGGTTTTAATTATGGTATGATACTTATTCCGGAAGGCCTTATTGACTTCATCCCGGAGGTACAGCAACTTATTGCAGAGCTAAATGAAATTCTGGCCCATGAAATAGTTGATGATGAAGGATTGTGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGCTCTTGCCTCGAGCAATCCAAGAACAATTGATGCTTGAGAGAGATCCACATGGAAATGTTCAGGTAGCTAAAATAGAAACAGAGAAAATGCTCATTCAGATGGTTGAAACAGAGTTGGAGAAGAGGAAGAGTGAAGGTGCATACAATGGTCAATTTAAAGGGCAATCTCACTTTTTTGGATATGAAGGGAGATGTGGTCTACCAACCAATTTCGATTCTACTTACTGCTATGCATTGGGTTATGGTGCTGGATCTCTCCTGCAGAGTGGAAAAACTGGACTAATATCATCTGTGGGGAACTTGGCTGCTCCCGTTGAAGAATGGACTGTTGGTGGGACTTCATTGACATCATTAATGGATGTGGAGAGGAGACATGGCAAATTTAAGCCTGTGATTAAGAAGGCAATGGTCGAGATTGATGGTACCCCTTTCAAGAAGTATGCATCATTGAGGGATGAGTGGGCATTCAACAACCGATACATTAGTCCTGGTCCTATTCAATTTGTGGGTCCTGCATCAAATGCTATTAATCATACTCTCCTCCTGGAGCTTGGAGCTGGAGCTTAA

Coding sequence (CDS)

ATGCCAAGTTCTTTCGTCTCCAATGGTGAAGCCGCCGTCAAATCTTCTCCTGCGGCGGTGTCCGGACGGGTCTCCTCAGTGTATGCTGAAGTTCAGTCCAGTCGTATCGACCACACTCTGCCTCTGCCTTCTGTGCTCAGAAGTTCCTTCACGATCGTCGATGGACCCCCTAGCTCTGCCGCTGGCAATCCAGACGAGATAGCCAAGCTATTTCCTAATCTTTTCGGCCAGCCATCGGCAAAATTGGTACAAAGCAATTCCAACAAAGCTCAACCGGACAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAACGTGATTTCCGGAATTTTTGATTATTTGCAGGATCATGTCAAAGGCAGTGTGTTATATGGCTTCAAGGGTGGTCCTGCCGGGATTATGAAATGTAATTATGTGGAATTGAATTCCGAATATATTCATCCATATAGAAATCAGGGTGGATTTGATATGATATGCAGTGGGAGGGACAAATTTGAAACAACAGAGCAGTTCAAACAAGCTGAAGAAACTGCAAAAAAGCTTGATTTGGATGGGATTGTTGTGATTGGTGGAGATGATTCCAATACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGAGTAAAAATTTGAAGACCCGGGTTATTGGTTGCCCCAAAACAATTGATGGAGATCTAAAATGCAAAGAGGTGCCCATAAGTTTTGGATTTGATACAGCTTGCAGGATTTATTCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTTTGTGAGGCTTATGGGACGTGCTGCTTCACACATCACCCTAGAGTGTGCTCTGCAGACTCATCCAAATATTACTATTATTGGGGAAGAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTCACAGATTACATTGTAGATGTAGTCTGCAAACGTGCAGAACTTGGTTTTAATTATGGTATGATACTTATTCCGGAAGGCCTTATTGACTTCATCCCGGAGGTACAGCAACTTATTGCAGAGCTAAATGAAATTCTGGCCCATGAAATAGTTGATGATGAAGGATTGTGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGCTCTTGCCTCGAGCAATCCAAGAACAATTGATGCTTGAGAGAGATCCACATGGAAATGTTCAGGTAGCTAAAATAGAAACAGAGAAAATGCTCATTCAGATGGTTGAAACAGAGTTGGAGAAGAGGAAGAGTGAAGGTGCATACAATGGTCAATTTAAAGGGCAATCTCACTTTTTTGGATATGAAGGGAGATGTGGTCTACCAACCAATTTCGATTCTACTTACTGCTATGCATTGGGTTATGGTGCTGGATCTCTCCTGCAGAGTGGAAAAACTGGACTAATATCATCTGTGGGGAACTTGGCTGCTCCCGTTGAAGAATGGACTGTTGGTGGGACTTCATTGACATCATTAATGGATGTGGAGAGGAGACATGGCAAATTTAAGCCTGTGATTAAGAAGGCAATGGTCGAGATTGATGGTACCCCTTTCAAGAAGTATGCATCATTGAGGGATGAGTGGGCATTCAACAACCGATACATTAGTCCTGGTCCTATTCAATTTGTGGGTCCTGCATCAAATGCTATTAATCATACTCTCCTCCTGGAGCTTGGAGCTGGAGCTTAA

Protein sequence

MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPASNAINHTLLLELGAGA
Homology
BLAST of CmaCh07G003320 vs. ExPASy Swiss-Prot
Match: Q41141 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus communis OX=3988 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 6.3e-278
Identity = 464/547 (84.83%), Postives = 503/547 (91.96%), Query Frame = 0

Query: 21  SGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSA 80
           SGR +SVY+EVQSSRI+H LPLPSVL   F IV GPPSSAAGNPDEIAKLFPNLFGQPSA
Sbjct: 6   SGRAASVYSEVQSSRIEHVLPLPSVLNHPFKIVQGPPSSAAGNPDEIAKLFPNLFGQPSA 65

Query: 81  KLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 140
            LV   ++    +++LKIG+VLSGGQAPGGHNVISGIFDYLQD  KGS+LYGF+GGPAGI
Sbjct: 66  MLVPDVADSLDSNQQLKIGLVLSGGQAPGGHNVISGIFDYLQDRAKGSILYGFRGGPAGI 125

Query: 141 MKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 200
           MKCNYV+L ++YIHPYRNQGGFDMICSGRDK ET EQFKQAEETA KLDL+G+VVIGGDD
Sbjct: 126 MKCNYVQLTADYIHPYRNQGGFDMICSGRDKIETPEQFKQAEETAGKLDLNGLVVIGGDD 185

Query: 201 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMID 260
           SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVP SFGFDTAC+IYSEMIGNVMID
Sbjct: 186 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYSEMIGNVMID 245

Query: 261 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCK 320
           ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV+A+K  LK VTDYIVDV+CK
Sbjct: 246 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLALKDVTDYIVDVICK 305

Query: 321 RAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 380
           RA+LG+NYG+ILIPEGLIDFIPEVQ LIAELNEILAH++VD+ GLWKKKLTSQSL+LFE 
Sbjct: 306 RADLGYNYGVILIPEGLIDFIPEVQNLIAELNEILAHDVVDEGGLWKKKLTSQSLQLFEF 365

Query: 381 LPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGY 440
           LP AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRK +G Y   FKGQSHFFGY
Sbjct: 366 LPVAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQQGTYKAHFKGQSHFFGY 425

Query: 441 EGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDV 500
           EGRCGLPTNFDSTYCYALGY AG+LL SGKTGLISSVGNL APV EWTVGGT+LTSLMDV
Sbjct: 426 EGRCGLPTNFDSTYCYALGYAAGALLHSGKTGLISSVGNLGAPVAEWTVGGTALTSLMDV 485

Query: 501 ERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPASNAINHTLL 560
           ERRHGKFKPVIKKAMVE++G PFKK+ASLR+EWA  NRY+SPGPIQF+GP S+A +HTLL
Sbjct: 486 ERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYVSPGPIQFMGPGSDAASHTLL 545

Query: 561 LELGAGA 568
           LELG+ A
Sbjct: 546 LELGSVA 552

BLAST of CmaCh07G003320 vs. ExPASy Swiss-Prot
Match: Q8W4M5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA1 PE=1 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 9.1e-277
Identity = 470/564 (83.33%), Postives = 512/564 (90.78%), Query Frame = 0

Query: 2   PSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAA 61
           P+  V+    AV S   A +   +SVY+EVQSSRI++TLPLPSVL+ +F IV+GP SSAA
Sbjct: 3   PALAVTRDLTAVGSPENAPAKGRASVYSEVQSSRINNTLPLPSVLKGAFKIVEGPASSAA 62

Query: 62  GNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           GNPDEIAKLFP L+GQPS  +V  + +      KLKIGVVLSGGQAPGGHNVISG+FDYL
Sbjct: 63  GNPDEIAKLFPGLYGQPSVAVV-PDQDAPSSAPKLKIGVVLSGGQAPGGHNVISGLFDYL 122

Query: 122 QDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQA 181
           Q+  KGS  YGFKGGPAGIMKC YVELN+EYI PYRNQGGFDMICSGRDK ET +QFKQA
Sbjct: 123 QERAKGSTFYGFKGGPAGIMKCKYVELNAEYIQPYRNQGGFDMICSGRDKIETPDQFKQA 182

Query: 182 EETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISF 241
           EETAKKLDLDG+VVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVP SF
Sbjct: 183 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSF 242

Query: 242 GFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 301
           GFDTAC+IYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS
Sbjct: 243 GFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 302

Query: 302 AQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVD 361
           AQKQTLK+VTDY+VDV+CKRAELG+NYG+ILIPEGLIDFIPEVQ+LIAELNEILA+E+VD
Sbjct: 303 AQKQTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQELIAELNEILANEVVD 362

Query: 362 DEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 421
           + GLWKKKLT QSL+LF+LLP AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR
Sbjct: 363 ENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 422

Query: 422 KSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLA 481
           K  GAY GQF GQSHFFGYEGRCGLPTNFD+TYCYALGYGAG LL SGKTGLISSVGNLA
Sbjct: 423 KQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLA 482

Query: 482 APVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYIS 541
           APVEEWTVGGT+LT+LMDVERRHGKFKPVIKKAMVE++G PFKK+ASLR+EWA  NRYIS
Sbjct: 483 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYIS 542

Query: 542 PGPIQFVGPASNAINHTLLLELGA 566
           PGPIQF GP S++++HTLLLELGA
Sbjct: 543 PGPIQFTGPGSDSLSHTLLLELGA 565

BLAST of CmaCh07G003320 vs. ExPASy Swiss-Prot
Match: P21343 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum tuberosum OX=4113 GN=PFP-BETA PE=1 SV=3)

HSP 1 Score: 946.0 bits (2444), Expect = 1.9e-274
Identity = 462/564 (81.91%), Postives = 511/564 (90.60%), Query Frame = 0

Query: 4   SFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAAGN 63
           S ++NGE A  S  +  +GR ++VY+EVQ+SR+DH LPLPSVL S F +VDGPPSSAAG+
Sbjct: 7   SLLNNGELA-SSVKSPGTGRYAAVYSEVQNSRLDHPLPLPSVLGSPFKVVDGPPSSAAGH 66

Query: 64  PDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQD 123
           P+EIAKLFP+L+GQP   LV  +S     ++ LKIGVVLSGGQAPGGHNVISGIFDYLQ 
Sbjct: 67  PEEIAKLFPSLYGQPCVSLVPDDSGDVAMNQILKIGVVLSGGQAPGGHNVISGIFDYLQT 126

Query: 124 HVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEE 183
           H KGS +YGF+GGPAG+MK  YV L  E+I+PYRNQGGFDMICSGRDK ET EQFKQAEE
Sbjct: 127 HCKGSTMYGFRGGPAGVMKGKYVVLTPEFIYPYRNQGGFDMICSGRDKIETPEQFKQAEE 186

Query: 184 TAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGF 243
           TAKKLDLDG+VVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLK KEVP SFGF
Sbjct: 187 TAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKSKEVPTSFGF 246

Query: 244 DTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQ 303
           DTAC+IY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPN+T+IGEEV A+
Sbjct: 247 DTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNVTLIGEEVFAK 306

Query: 304 KQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDE 363
           K TLK+VTDYI DVVCKRAE G+NYG+ILIPEGLIDFIPEVQQLIAELNEILAH++VD+ 
Sbjct: 307 KLTLKNVTDYIADVVCKRAESGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDEA 366

Query: 364 GLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKS 423
           G+WKKKLT Q LELFELLP AIQEQL+LERDPHGNVQVAKIETEKMLIQMVETEL++RK 
Sbjct: 367 GVWKKKLTPQCLELFELLPLAIQEQLLLERDPHGNVQVAKIETEKMLIQMVETELDQRKQ 426

Query: 424 EGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAP 483
           +GAYN QFKGQSHFFGYEGRCGLP+NFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAP
Sbjct: 427 KGAYNAQFKGQSHFFGYEGRCGLPSNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAP 486

Query: 484 VEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPG 543
           VEEWTVGGT+LT+LMDVERRHGKFKPVIKKAMVE++G PFKK+AS R+EWA NNRYI+PG
Sbjct: 487 VEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASKREEWALNNRYINPG 546

Query: 544 PIQFVGPASNAINHTLLLELGAGA 568
           PIQFVGP +N +NHTLLLELG  A
Sbjct: 547 PIQFVGPVANKVNHTLLLELGVDA 569

BLAST of CmaCh07G003320 vs. ExPASy Swiss-Prot
Match: F4JGR5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA2 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 1.6e-265
Identity = 443/547 (80.99%), Postives = 496/547 (90.68%), Query Frame = 0

Query: 21  SGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSA 80
           + RV+SVY+EVQ+SRIDHTLPLPSV ++ F I+DGPPSS+AG+P+EI KLFPNLFGQPSA
Sbjct: 23  NSRVTSVYSEVQASRIDHTLPLPSVFKTPFKIIDGPPSSSAGHPEEIEKLFPNLFGQPSA 82

Query: 81  KLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 140
            LV + SN+   D+KLKIGVVLSGGQAPGGHNVI GIFDYLQ++ +GS L+GF+GGPAGI
Sbjct: 83  LLVPNQSNEVSSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQEYARGSSLFGFRGGPAGI 142

Query: 141 MKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 200
           MK  Y+EL SE+++PYRNQGGFDMICSGRDK ET EQFKQAEET  K+DLDG+VVIGGDD
Sbjct: 143 MKGKYIELTSEFVYPYRNQGGFDMICSGRDKIETPEQFKQAEETVTKMDLDGLVVIGGDD 202

Query: 201 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMID 260
           SNTNACLLAE+FR+KN+KT VIGCPKTIDGDLK KEVP SFGFDTAC+IYSEMIGNVMID
Sbjct: 203 SNTNACLLAEHFRAKNMKTLVIGCPKTIDGDLKSKEVPTSFGFDTACKIYSEMIGNVMID 262

Query: 261 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCK 320
           ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV  +K TLK+VTD IVDV+ K
Sbjct: 263 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVFEKKLTLKNVTDNIVDVIYK 322

Query: 321 RAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 380
           RAE G+NYG+IL+PEGLIDFIPEVQQLI+ELNE+LA   VD+EG WKK L  ++LE+FE 
Sbjct: 323 RAENGYNYGVILVPEGLIDFIPEVQQLISELNEVLAEGNVDEEGQWKKNLKKETLEIFEF 382

Query: 381 LPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGY 440
           LP+ IQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK+K+EG Y  +F G+SHFFGY
Sbjct: 383 LPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTEGTYEREFMGKSHFFGY 442

Query: 441 EGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDV 500
           EGRCGLPTNFD+TYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGT+LTSLMDV
Sbjct: 443 EGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDV 502

Query: 501 ERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPASNAINHTLL 560
           ERRHGKFKPVIKKAMVE++G PFKK+AS R+EWA  NRYISPGPIQF GP S+A NHTL+
Sbjct: 503 ERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGPGSDARNHTLM 562

Query: 561 LELGAGA 568
           LELGA A
Sbjct: 563 LELGAQA 569

BLAST of CmaCh07G003320 vs. ExPASy Swiss-Prot
Match: Q9EZ02 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1) OX=665571 GN=pfp PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 8.0e-156
Identity = 278/548 (50.73%), Postives = 374/548 (68.25%), Query Frame = 0

Query: 31  VQSSRIDHTLPLPSVLRSSFTIVD---GPPSSAAGNPDEIAKLFPNLFGQPSAKLVQSNS 90
           +Q +R  +   LP VL+     +    G P+ A  + +++ +LF N +G+P A L + ++
Sbjct: 7   LQKARYGYVPKLPPVLQDEIARIQAELGQPTEAVADREDLKRLFANTYGKPIATLTRGSN 66

Query: 91  NKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVE 150
            +A   ++  +GV+LSGG APGGHNVI G+FD L+   + S L GFKGGP+GI+   ++E
Sbjct: 67  PEA--GRRRTVGVILSGGPAPGGHNVICGLFDALKKANRESTLIGFKGGPSGILDDEWIE 126

Query: 151 LNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACL 210
                I+ YRN GGFD+I SGR K ET EQF +A E AKK  LD +V+IGGDDSNTNA L
Sbjct: 127 FTDSLINQYRNTGGFDIIGSGRTKIETPEQFAKALENAKKHGLDALVIIGGDDSNTNAAL 186

Query: 211 LAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMIDARSTGKY 270
           LAE F  +    +VIG PKTIDGDLK + +  SFGFDTA ++Y+E+IGN+  DA S+ KY
Sbjct: 187 LAEYFVQQGAPIQVIGIPKTIDGDLKNEYIEASFGFDTATKVYAELIGNIARDAISSRKY 246

Query: 271 YHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCKRAELGFN 330
           +HF+RLMGR+ASHI LECALQTHPN+ I+ EEV  +  TL  + D IVD V KRA  G N
Sbjct: 247 WHFIRLMGRSASHIALECALQTHPNVCIVSEEVREKNMTLSQIVDQIVDAVVKRAAKGEN 306

Query: 331 YGMILIPEGLIDFIPEVQQLIAELNEILAHEI-----VDDEG---LW-KKKLTSQSLELF 390
           +G++L+PEGLI+FIPEV  LI ELN +LA E      +DD      W K KL+  +  +F
Sbjct: 307 FGVVLVPEGLIEFIPEVGALIDELNTLLAKEAEVFNRIDDPRERISWVKGKLSGNNQHVF 366

Query: 391 ELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFF 450
             LP  IQ QL+++RDPHGNVQV++IETEK+LI+MV + L+  K EGAY G+F   +HFF
Sbjct: 367 SSLPETIQAQLLMDRDPHGNVQVSRIETEKLLIEMVSSRLKALKEEGAYKGKFSALNHFF 426

Query: 451 GYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLM 510
           GYE RC  P+NFD+ YCYALG+ A  L+ +G TG I+++ NLA P  EW   G  LT +M
Sbjct: 427 GYEARCAFPSNFDADYCYALGFTAFVLIANGLTGYIAAIKNLARPAVEWKPMGIPLTMMM 486

Query: 511 DVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPASNAINHT 567
           ++E+RHGK KPVI+KA+V+++G PFK++A+ RD WA  + Y+ PG IQ+ GP       T
Sbjct: 487 NMEKRHGKMKPVIRKALVDLEGAPFKRFATQRDAWATESAYVFPGAIQYYGPDDVCNQPT 546

BLAST of CmaCh07G003320 vs. ExPASy TrEMBL
Match: A0A6J1KXF5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111497221 PE=3 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGAGA
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAGA 567

BLAST of CmaCh07G003320 vs. ExPASy TrEMBL
Match: A0A6J1EGM8 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111433922 PE=3 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 549/567 (96.83%), Postives = 556/567 (98.06%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           MPSS VSNGEAAVKSSPAAVSGRVSSVYAEVQSSRID  LPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MPSSIVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S SNKAQ DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVEL +EYI+PYRNQGGFDMICSGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELGFNYG+ILIPEGLIDFIPEVQQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGVILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RK E AYN QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKRESAYNRQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVE+DGTPFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGAGA
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAGA 567

BLAST of CmaCh07G003320 vs. ExPASy TrEMBL
Match: A0A6J1GG11 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111453824 PE=3 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.1e-310
Identity = 530/567 (93.47%), Postives = 550/567 (97.00%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+PA V+GRVSSVY+EVQSSRIDH LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S+SNK QPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGF+GGPAGIMKC YVEL SEYI+PYRNQGGFDMICSGRDK ET EQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELG+NYG+ILIPEGLIDFIPEVQQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVE+DG PFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGA A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of CmaCh07G003320 vs. ExPASy TrEMBL
Match: A0A6J1IR64 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111478001 PE=3 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 4.6e-308
Identity = 526/567 (92.77%), Postives = 548/567 (96.65%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+ A V+GRVSSVY+EVQSSRIDH LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNHAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S+SNK QPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVK SVLYGF+GGPAGIMKC YVEL SEYI+PYRNQGGFDMICSGRDK ET EQFKQ
Sbjct: 121 LQDHVKSSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVM+DARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMVDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELG+NYG+ILIPEGLIDFIPEVQQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RK+EGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKNEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVE+DG PFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGA A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of CmaCh07G003320 vs. ExPASy TrEMBL
Match: A0A0A0KH95 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis sativus OX=3659 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.5e-306
Identity = 522/567 (92.06%), Postives = 545/567 (96.12%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           M  SF+SNGEAAVKSS   +SGRVSSVY+EVQSSRI+H LPLPSVLRS FTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVKSSSPPLSGRVSSVYSEVQSSRINHNLPLPSVLRSPFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S+SNK QPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKGQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDH KGSVLYGF+GGPAGIMKC Y+EL S+YI+PYRNQGGFDMICSGRDK ET EQFKQ
Sbjct: 121 LQDHAKGSVLYGFRGGPAGIMKCKYLELTSDYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDG+VVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
            AQKQTLKSVTDYIVDVVCKRAELG+NYG+ILIPEGLIDFIPEVQQLIAELNEILAH++V
Sbjct: 301 YAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFE LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAY GQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVE+DG PFKK+ASLRD+WAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDDWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNA+NHTLLLELG  A
Sbjct: 541 SPGPIQFVGPASNAVNHTLLLELGVEA 567

BLAST of CmaCh07G003320 vs. NCBI nr
Match: XP_023003713.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucurbita maxima])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGAGA
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAGA 567

BLAST of CmaCh07G003320 vs. NCBI nr
Match: XP_023518798.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 550/567 (97.00%), Postives = 559/567 (98.59%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRID  LPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S SNKAQ DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVEL +EYI+PYRNQGGFDMI SGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELGFNYG+ILIPEGLIDFIPEVQQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGVILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVE+DGTPFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPAS+AINHTLLLELGAGA
Sbjct: 541 SPGPIQFVGPASSAINHTLLLELGAGA 567

BLAST of CmaCh07G003320 vs. NCBI nr
Match: XP_022926964.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucurbita moschata] >KAG6594681.1 Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026650.1 Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 549/567 (96.83%), Postives = 556/567 (98.06%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           MPSS VSNGEAAVKSSPAAVSGRVSSVYAEVQSSRID  LPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MPSSIVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S SNKAQ DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVEL +EYI+PYRNQGGFDMICSGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELGFNYG+ILIPEGLIDFIPEVQQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGVILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RK E AYN QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKRESAYNRQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVE+DGTPFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGAGA
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAGA 567

BLAST of CmaCh07G003320 vs. NCBI nr
Match: XP_022950848.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita moschata])

HSP 1 Score: 1073.5 bits (2775), Expect = 4.4e-310
Identity = 530/567 (93.47%), Postives = 550/567 (97.00%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+PA V+GRVSSVY+EVQSSRIDH LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S+SNK QPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGF+GGPAGIMKC YVEL SEYI+PYRNQGGFDMICSGRDK ET EQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELG+NYG+ILIPEGLIDFIPEVQQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVE+DG PFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGA A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of CmaCh07G003320 vs. NCBI nr
Match: KAG7033973.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.9e-309
Identity = 529/567 (93.30%), Postives = 549/567 (96.83%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+PA V+GRVSSVY+EVQSSRIDH LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S+SNK QP KKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPGKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGF+GGPAGIMKC YVEL SEYI+PYRNQGGFDMICSGRDK ET EQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360
           SAQKQTLKSVTDYIVDVVCKRAELG+NYG+ILIPEGLIDFIPEVQQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480
           RKSEGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVE+DG PFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAINHTLLLELGAGA 568
           SPGPIQFVGPASNAINHTLLLELGA A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of CmaCh07G003320 vs. TAIR 10
Match: AT1G12000.1 (Phosphofructokinase family protein )

HSP 1 Score: 953.7 bits (2464), Expect = 6.5e-278
Identity = 470/564 (83.33%), Postives = 512/564 (90.78%), Query Frame = 0

Query: 2   PSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAA 61
           P+  V+    AV S   A +   +SVY+EVQSSRI++TLPLPSVL+ +F IV+GP SSAA
Sbjct: 3   PALAVTRDLTAVGSPENAPAKGRASVYSEVQSSRINNTLPLPSVLKGAFKIVEGPASSAA 62

Query: 62  GNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           GNPDEIAKLFP L+GQPS  +V  + +      KLKIGVVLSGGQAPGGHNVISG+FDYL
Sbjct: 63  GNPDEIAKLFPGLYGQPSVAVV-PDQDAPSSAPKLKIGVVLSGGQAPGGHNVISGLFDYL 122

Query: 122 QDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQA 181
           Q+  KGS  YGFKGGPAGIMKC YVELN+EYI PYRNQGGFDMICSGRDK ET +QFKQA
Sbjct: 123 QERAKGSTFYGFKGGPAGIMKCKYVELNAEYIQPYRNQGGFDMICSGRDKIETPDQFKQA 182

Query: 182 EETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISF 241
           EETAKKLDLDG+VVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVP SF
Sbjct: 183 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSF 242

Query: 242 GFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 301
           GFDTAC+IYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS
Sbjct: 243 GFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 302

Query: 302 AQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVD 361
           AQKQTLK+VTDY+VDV+CKRAELG+NYG+ILIPEGLIDFIPEVQ+LIAELNEILA+E+VD
Sbjct: 303 AQKQTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQELIAELNEILANEVVD 362

Query: 362 DEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 421
           + GLWKKKLT QSL+LF+LLP AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR
Sbjct: 363 ENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 422

Query: 422 KSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLA 481
           K  GAY GQF GQSHFFGYEGRCGLPTNFD+TYCYALGYGAG LL SGKTGLISSVGNLA
Sbjct: 423 KQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLA 482

Query: 482 APVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYIS 541
           APVEEWTVGGT+LT+LMDVERRHGKFKPVIKKAMVE++G PFKK+ASLR+EWA  NRYIS
Sbjct: 483 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYIS 542

Query: 542 PGPIQFVGPASNAINHTLLLELGA 566
           PGPIQF GP S++++HTLLLELGA
Sbjct: 543 PGPIQFTGPGSDSLSHTLLLELGA 565

BLAST of CmaCh07G003320 vs. TAIR 10
Match: AT4G04040.1 (Phosphofructokinase family protein )

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-266
Identity = 443/547 (80.99%), Postives = 496/547 (90.68%), Query Frame = 0

Query: 21  SGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSA 80
           + RV+SVY+EVQ+SRIDHTLPLPSV ++ F I+DGPPSS+AG+P+EI KLFPNLFGQPSA
Sbjct: 23  NSRVTSVYSEVQASRIDHTLPLPSVFKTPFKIIDGPPSSSAGHPEEIEKLFPNLFGQPSA 82

Query: 81  KLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 140
            LV + SN+   D+KLKIGVVLSGGQAPGGHNVI GIFDYLQ++ +GS L+GF+GGPAGI
Sbjct: 83  LLVPNQSNEVSSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQEYARGSSLFGFRGGPAGI 142

Query: 141 MKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 200
           MK  Y+EL SE+++PYRNQGGFDMICSGRDK ET EQFKQAEET  K+DLDG+VVIGGDD
Sbjct: 143 MKGKYIELTSEFVYPYRNQGGFDMICSGRDKIETPEQFKQAEETVTKMDLDGLVVIGGDD 202

Query: 201 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMID 260
           SNTNACLLAE+FR+KN+KT VIGCPKTIDGDLK KEVP SFGFDTAC+IYSEMIGNVMID
Sbjct: 203 SNTNACLLAEHFRAKNMKTLVIGCPKTIDGDLKSKEVPTSFGFDTACKIYSEMIGNVMID 262

Query: 261 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCK 320
           ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV  +K TLK+VTD IVDV+ K
Sbjct: 263 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVFEKKLTLKNVTDNIVDVIYK 322

Query: 321 RAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 380
           RAE G+NYG+IL+PEGLIDFIPEVQQLI+ELNE+LA   VD+EG WKK L  ++LE+FE 
Sbjct: 323 RAENGYNYGVILVPEGLIDFIPEVQQLISELNEVLAEGNVDEEGQWKKNLKKETLEIFEF 382

Query: 381 LPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGY 440
           LP+ IQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK+K+EG Y  +F G+SHFFGY
Sbjct: 383 LPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTEGTYEREFMGKSHFFGY 442

Query: 441 EGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDV 500
           EGRCGLPTNFD+TYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGT+LTSLMDV
Sbjct: 443 EGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDV 502

Query: 501 ERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPASNAINHTLL 560
           ERRHGKFKPVIKKAMVE++G PFKK+AS R+EWA  NRYISPGPIQF GP S+A NHTL+
Sbjct: 503 ERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGPGSDARNHTLM 562

Query: 561 LELGAGA 568
           LELGA A
Sbjct: 563 LELGAQA 569

BLAST of CmaCh07G003320 vs. TAIR 10
Match: AT1G76550.1 (Phosphofructokinase family protein )

HSP 1 Score: 389.4 bits (999), Expect = 4.9e-108
Identity = 215/547 (39.31%), Postives = 320/547 (58.50%), Query Frame = 0

Query: 31  VQSSRIDHTLPLPSVLRSSFTIV---DGPPSSAAGNPDEIAKLFPNLFGQPSAKLVQSNS 90
           +Q  R  +   LP  L+ +   V   DG   + AG+   IA+ FP+  GQP A  +++ +
Sbjct: 14  LQQLRSQYHPELPPCLQGTTVRVELGDGTTVAKAGDAHIIARAFPHTLGQPLAHFLRATA 73

Query: 91  NKAQPD-----KKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMK 150
             A           ++G+V  G QAPGGHNV+ G+++ L+ H   + L GF GG  G+  
Sbjct: 74  KVADAQIITEHPVKRVGIVFCGRQAPGGHNVVWGLYEALKVHNAKNTLLGFLGGSEGLFA 133

Query: 151 CNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSN 210
              +E+  E +  Y+NQGG+DM+   +D+  TTEQ   A +    L LD +V+IGG  SN
Sbjct: 134 QKTLEITDEVLQTYKNQGGYDMLGRTKDQIRTTEQVNAALKACTDLKLDSLVIIGGVTSN 193

Query: 211 TNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMIDAR 270
           T+A  LAE F      T+V+G P TI+GDLK + V  + GFDT C++ S++I N+  DA 
Sbjct: 194 TDAAHLAEFFAEAKCSTKVVGVPVTINGDLKNQFVEANVGFDTTCKVNSQLISNICTDAL 253

Query: 271 STGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCKRA 330
           S  KYY+FVRLMGR  SH+ LEC LQ+HPN+ I+GEEV+A K T+  +   I D V  RA
Sbjct: 254 SAEKYYYFVRLMGRKHSHVALECTLQSHPNMVILGEEVTASKLTIFDIIKQICDAVQARA 313

Query: 331 ELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFELLP 390
           E   N+G+ILIPEGL++ IPE+  L+ E++ +L   +  D      +L+S S  LFE LP
Sbjct: 314 EQDKNHGVILIPEGLVESIPELYALLKEIHGLLKEGVQVDN--ISTQLSSWSSALFEFLP 373

Query: 391 RAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNG-QFKGQSHFFGYE 450
             I++QL+L  +   + Q+++IETEK+L  +VETE+ KR  EG Y G +F    HFFGY+
Sbjct: 374 PFIKKQLLLHPESDDSAQLSQIETEKLLAYLVETEMNKRLKEGTYKGKKFNAICHFFGYQ 433

Query: 451 GRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDVE 510
            R  LP+ FD  Y Y LG+    +L +G  G +++V NL +PV +W  G   ++++M V+
Sbjct: 434 ARGSLPSKFDCDYAYVLGHVCYHILAAGLNGYMATVTNLKSPVNKWKCGAAPISAMMTVK 493

Query: 511 R---RHGKF---KPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPASNAI 563
           R     G     +PVI  A V++ G  +        ++   + Y +PGP+Q+ GP ++A 
Sbjct: 494 RWSQNSGSTTIGRPVIHPASVDLKGKAYDLLRQNAQKFLMEDMYRNPGPVQYDGPGADAK 553

BLAST of CmaCh07G003320 vs. TAIR 10
Match: AT1G20950.1 (Phosphofructokinase family protein )

HSP 1 Score: 381.7 bits (979), Expect = 1.0e-105
Identity = 214/551 (38.84%), Postives = 320/551 (58.08%), Query Frame = 0

Query: 31  VQSSRIDHTLPLPSVLRSSFTIV---DGPPSSAAGNPDEIAKLFPNLFGQPSAKLVQSNS 90
           +Q  R  +   LP  L+ +   V   DG   + A +   +A+ FP+  GQP A  ++  +
Sbjct: 14  LQQLRSQYKPELPPCLQGTTVRVELGDGTTVAEAADSHTMARAFPHTLGQPLAHFLRETA 73

Query: 91  NKAQPD-------KKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 150
               PD         +++G+V  G QAPGGHNVI G+F+ L+ H   S L GF GG  G+
Sbjct: 74  Q--VPDAHIITELPSVRVGIVFCGRQAPGGHNVIWGLFEALKVHNAKSTLLGFLGGSEGL 133

Query: 151 MKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 210
                +E+  + +  Y+NQGG+D++   +D+ +TTEQ   A +    L LDG+V+IGG  
Sbjct: 134 FAQKTLEITDDILQTYKNQGGYDLLGRTKDQIKTTEQVNAALKACTDLKLDGLVIIGGVI 193

Query: 211 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMID 270
           SNT+A  LAE F +    T+V+G P T +GDLK + V  + GFDT C++ S++I N   D
Sbjct: 194 SNTDAAHLAEFFAAAKCSTKVVGVPVTTNGDLKNQFVEANVGFDTICKVNSQLISNACTD 253

Query: 271 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVVCK 330
           A S  KYY+F+RLMGR  SH+ LEC LQ+HPN+ I+GEEV+A K T+  +   I D V  
Sbjct: 254 ALSAEKYYYFIRLMGRKHSHVALECTLQSHPNMVILGEEVAASKLTIFDIAKQICDAVQA 313

Query: 331 RAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 390
           RA    N+G+ILIPEGLI  IPEV  L+ E++ +L   +  D+     +L+  S  LFE 
Sbjct: 314 RAVEDKNHGVILIPEGLIVSIPEVYALLKEIHGLLRQGVSADK--ISTQLSPWSSALFEF 373

Query: 391 LPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNG-QFKGQSHFFG 450
           LP  I++QL+L  +   + Q+++IETEK+L  +VETE+ KR  EG Y G +F    HFFG
Sbjct: 374 LPPFIKKQLLLHPESDDSAQLSQIETEKLLAYLVETEMNKRLKEGTYKGKKFNAICHFFG 433

Query: 451 YEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMD 510
           Y+ R  LP+ FD  Y Y LG+    +L +G  G +++V NL +PV +W  G T +T++M 
Sbjct: 434 YQARGSLPSKFDCDYAYVLGHICYHILAAGLNGYMATVTNLKSPVNKWKCGATPITAMMT 493

Query: 511 VER--RHGKF------KPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYISPGPIQFVGPA 563
           V+   +   +      +P I  AMV++ G  +        ++   + Y +PGP+Q+ GP 
Sbjct: 494 VKHWSQDASYTLTSIGRPAIHPAMVDLKGKAYDLLRQNAQKFLMEDLYRNPGPLQYDGPG 553

BLAST of CmaCh07G003320 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 89.7 bits (221), Expect = 8.0e-18
Identity = 56/188 (29.79%), Postives = 88/188 (46.81%), Query Frame = 0

Query: 100 VVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQ 159
           +V  GG  PG + VI  +   L        + G  GG  G    N + LNS+ ++    +
Sbjct: 96  IVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAKNTIPLNSKVVNDIHKR 155

Query: 160 GGFDMICSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKT 219
           GG  +I + R   +T +     ++      ++ + +IGGD +   A ++ E  R + LK 
Sbjct: 156 GG-TIIGTSRGGHDTNKIVDSIQDRG----INQVYIIGGDGTQRGASVIFEEIRRRRLKV 215

Query: 220 RVIGCPKTIDGDLKCKEVPISFGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAAS 279
            V+G PKTID D+    +  SFGFDTA       I    ++A S      FV+LMGR + 
Sbjct: 216 AVVGIPKTIDNDIPV--IDKSFGFDTAVEEAQRAINAAHVEAESNENGIGFVKLMGRYSG 275

Query: 280 HITLECAL 288
           +I +   L
Sbjct: 276 YIAMYATL 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q411416.3e-27884.83Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus... [more]
Q8W4M59.1e-27783.33Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabi... [more]
P213431.9e-27481.91Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum... [more]
F4JGR51.6e-26580.99Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabi... [more]
Q9EZ028.0e-15650.73Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Spirochaeta thermoph... [more]
Match NameE-valueIdentityDescription
A0A6J1KXF50.0e+00100.00Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1EGM80.0e+0096.83Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1GG112.1e-31093.47Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1IR644.6e-30892.77Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A0A0KH951.5e-30692.06Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
Match NameE-valueIdentityDescription
XP_023003713.10.0e+00100.00pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucu... [more]
XP_023518798.10.0e+0097.00pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucu... [more]
XP_022926964.10.0e+0096.83pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucu... [more]
XP_022950848.14.4e-31093.47pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
KAG7033973.13.9e-30993.30Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
Match NameE-valueIdentityDescription
AT1G12000.16.5e-27883.33Phosphofructokinase family protein [more]
AT4G04040.11.1e-26680.99Phosphofructokinase family protein [more]
AT1G76550.14.9e-10839.31Phosphofructokinase family protein [more]
AT1G20950.11.0e-10538.84Phosphofructokinase family protein [more]
AT5G56630.18.0e-1829.79phosphofructokinase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 325..337
score: 27.02
coord: 100..119
score: 32.56
coord: 189..205
score: 46.91
coord: 222..239
score: 34.9
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBPIRSFPIRSF005677PPi_PFK_PfpBcoord: 21..567
e-value: 1.1E-259
score: 860.4
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBTIGRFAMTIGR02477TIGR02477coord: 32..562
e-value: 4.2E-244
score: 808.9
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBHAMAPMF_01980Phosphofructokinase_II_Longcoord: 27..565
score: 42.684883
NoneNo IPR availableGENE3D3.40.50.450coord: 41..516
e-value: 4.5E-238
score: 792.9
NoneNo IPR availableGENE3D1.10.10.480Phosphofructokinase; domain 3coord: 341..407
e-value: 4.5E-238
score: 792.9
NoneNo IPR availablePANTHERPTHR43650PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASEcoord: 18..566
NoneNo IPR availablePANTHERPTHR43650:SF6PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT BETAcoord: 18..566
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 97..462
e-value: 4.5E-37
score: 127.9
IPR035966Phosphofructokinase superfamilyGENE3D3.40.50.460Phosphofructokinase domaincoord: 243..551
e-value: 4.5E-238
score: 792.9
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 31..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G003320.1CmaCh07G003320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0015979 photosynthesis
biological_process GO:0009749 response to glucose
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0047334 diphosphate-fructose-6-phosphate 1-phosphotransferase activity
molecular_function GO:0046872 metal ion binding