CmaCh06G015280 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G015280
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr06: 9624522 .. 9626176 (-)
RNA-Seq Expression	CmaCh06G015280
Synteny	CmaCh06G015280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCTCTAATGCTGACATTAAGACTCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAAAAGTCCTGGTGAAGTCCAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAGACAAGCCGCCACCGACCCCCTGGCGCAAAACGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTAATAAAATCACCGATAGTTTTGACGTACACAGAGTGAATGATTATCTTGAGAACCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAACAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGACGTCAAAAGAAATGACCGCCAATGGGCTTGTGATGGTGGGTGAGGTTACATCCCTTGTGACCACTTTGTGGCAGAAGCTGGGACTGCTACAAACGGGGCGGCAACTTCGAACAGAGCATTCCAATGAAGAAAAGGAAGAGCCATCTTGGGTGAGCGACAGACGAGGGCTACTCGAGGCCACTGGAGCTAACATCAAGGCGAATGTGGTGGTGGCTAAAGACGGGAGTGGAAAGTACAAGACAATAACTGAAGCCCTTAAAGAGGTACCATTGAAGAGCAATACAACCTTTGTGATTTATGTGAAGGAAGGAGTTTATGAAGAGCAAGTGATGGTGGACAAAAAAATGACCTATGTCATGGTGATTGGAGACGGCCCGACTAAGACCAAGATCACAGGTAGCAAGAATGTCGTCGATGGGACAACCACATTCAAAAGTGCTACCTTTGGTAAGTTGCCATCTAATTTATAGTCATATTCATACGAATTCAATAAGTGAATTTAACATGATTGCATATTCTTAACAGCGGCGGTCGGATCCAACTTCATCGGAAAAGACTTGTGGTTCGATAACTCAGCTGGACTTTACAAAGGCCAGGCCGTGGCACTCCGAGTTCAATCCGACATGTCAATCTTCTACAATTGTAGAATGGACGGCTATCAAGATACTTTCTACCCCCATGCCAACCGTCAATTCTATCGAGATTGCACAATCTCCGGTACCGTTGATTTCATCTTTGGCAATGCTGCTGTCGTGTTACAGAACTGTAAGATCTTAGTGAGGAAGCCAAAGCCGAAACAACCATGCCCTATCACTGCCCAAGGTCGAATGGAGAGTGATGAGCCCACTGGTATCGTCCTCCAAAATTGCACCATCTCTTCCGACCCTGACTACTACCCCGTTCGCCATACCAGTAAGTCCTACCTTGGTCGCCCATGGAAGCAATATTCAAGGACCGTCGTCATGCAAAGCCAAATCGACGACTTGATCCAGCCTGAAGGTTGGCTTCCATGGAATGGTGATTTTGCTCTCAATACTTTGTATTACACAGAGTTTGACAACAGAGGACCTGGTGCAGCAAAAGAAAATAGAGTTAAGTGGAAGGGAATTAAGCAGATCACCGCAAAAGAGGCCATCAATTTCACCCCTTCGTTGTTTATTCATGGTGATTCTTGGATCAAGAACACCGGAATATCCTACACGGGTGCCTTGATGAAAATCTAA

mRNA sequence

ATGGCTCCTCTAATGCTGACATTAAGACTCTATCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAGACAAGCCGCCACCGACCCCCTGGCGCAAAACGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTAATAAAATCACCGATAGTTTTGACGTACACAGAGTGAATGATTATCTTGAGAACCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAACAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGACGTCAAAAGAAATGACCGCCAATGGGCTTGTGATGGTGGGTGAGGTTACATCCCTTGTGACCACTTTGTGGCAGAAGCTGGGACTGCTACAAACGGGGCGGCAACTTCGAACAGAGCATTCCAATGAAGAAAAGGAAGAGCCATCTTGGGTGAGCGACAGACGAGGGCTACTCGAGGCCACTGGAGCTAACATCAAGGCGAATGTGGTGGTGGCTAAAGACGGGAGTGGAAAGTACAAGACAATAACTGAAGCCCTTAAAGAGGTACCATTGAAGAGCAATACAACCTTTGTGATTTATGTGAAGGAAGGAGTTTATGAAGAGCAAGTGATGGTGGACAAAAAAATGACCTATGTCATGGTGATTGGAGACGGCCCGACTAAGACCAAGATCACAGGTAGCAAGAATGTCGTCGATGGGACAACCACATTCAAAAGTGCTACCTTTGCGGCGGTCGGATCCAACTTCATCGGAAAAGACTTGTGGTTCGATAACTCAGCTGGACTTTACAAAGGCCAGGCCGTGGCACTCCGAGTTCAATCCGACATGTCAATCTTCTACAATTGTAGAATGGACGGCTATCAAGATACTTTCTACCCCCATGCCAACCGTCAATTCTATCGAGATTGCACAATCTCCGGTACCGTTGATTTCATCTTTGGCAATGCTGCTGTCGTGTTACAGAACTGTAAGATCTTAGTGAGGAAGCCAAAGCCGAAACAACCATGCCCTATCACTGCCCAAGGTCGAATGGAGAGTGATGAGCCCACTGGTATCGTCCTCCAAAATTGCACCATCTCTTCCGACCCTGACTACTACCCCGTTCGCCATACCAGTAAGTCCTACCTTGGTCGCCCATGGAAGCAATATTCAAGGACCGTCGTCATGCAAAGCCAAATCGACGACTTGATCCAGCCTGAAGGTTGGCTTCCATGGAATGGTGATTTTGCTCTCAATACTTTGTATTACACAGAGTTTGACAACAGAGGACCTGGTGCAGCAAAAGAAAATAGAGTTAAGTGGAAGGGAATTAAGCAGATCACCGCAAAAGAGGCCATCAATTTCACCCCTTCGTTGTTTATTCATGGTGATTCTTGGATCAAGAACACCGGAATATCCTACACGGGTGCCTTGATGAAAATCTAA

Coding sequence (CDS)

ATGGCTCCTCTAATGCTGACATTAAGACTCTATCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAGACAAGCCGCCACCGACCCCCTGGCGCAAAACGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTAATAAAATCACCGATAGTTTTGACGTACACAGAGTGAATGATTATCTTGAGAACCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAACAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGACGTCAAAAGAAATGACCGCCAATGGGCTTGTGATGGTGGGTGAGGTTACATCCCTTGTGACCACTTTGTGGCAGAAGCTGGGACTGCTACAAACGGGGCGGCAACTTCGAACAGAGCATTCCAATGAAGAAAAGGAAGAGCCATCTTGGGTGAGCGACAGACGAGGGCTACTCGAGGCCACTGGAGCTAACATCAAGGCGAATGTGGTGGTGGCTAAAGACGGGAGTGGAAAGTACAAGACAATAACTGAAGCCCTTAAAGAGGTACCATTGAAGAGCAATACAACCTTTGTGATTTATGTGAAGGAAGGAGTTTATGAAGAGCAAGTGATGGTGGACAAAAAAATGACCTATGTCATGGTGATTGGAGACGGCCCGACTAAGACCAAGATCACAGGTAGCAAGAATGTCGTCGATGGGACAACCACATTCAAAAGTGCTACCTTTGCGGCGGTCGGATCCAACTTCATCGGAAAAGACTTGTGGTTCGATAACTCAGCTGGACTTTACAAAGGCCAGGCCGTGGCACTCCGAGTTCAATCCGACATGTCAATCTTCTACAATTGTAGAATGGACGGCTATCAAGATACTTTCTACCCCCATGCCAACCGTCAATTCTATCGAGATTGCACAATCTCCGGTACCGTTGATTTCATCTTTGGCAATGCTGCTGTCGTGTTACAGAACTGTAAGATCTTAGTGAGGAAGCCAAAGCCGAAACAACCATGCCCTATCACTGCCCAAGGTCGAATGGAGAGTGATGAGCCCACTGGTATCGTCCTCCAAAATTGCACCATCTCTTCCGACCCTGACTACTACCCCGTTCGCCATACCAGTAAGTCCTACCTTGGTCGCCCATGGAAGCAATATTCAAGGACCGTCGTCATGCAAAGCCAAATCGACGACTTGATCCAGCCTGAAGGTTGGCTTCCATGGAATGGTGATTTTGCTCTCAATACTTTGTATTACACAGAGTTTGACAACAGAGGACCTGGTGCAGCAAAAGAAAATAGAGTTAAGTGGAAGGGAATTAAGCAGATCACCGCAAAAGAGGCCATCAATTTCACCCCTTCGTTGTTTATTCATGGTGATTCTTGGATCAAGAACACCGGAATATCCTACACGGGTGCCTTGATGAAAATCTAA

Protein sequence

MAPLMLTLRLYLKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI

Homology

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BLAST of CmaCh06G015280 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 507.3 bits (1305), Expect = 1.9e-142
Identity = 249/474 (52.53%), Postives = 338/474 (71.31%), Query Frame = 0


Query: 15  AIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRI 74
           A   S T+ +   D   + A+D C ELMDYA+D+L  SF ++   F+ H +++ L NLRI
Sbjct: 103 AAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRI 162

Query: 75  WLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLG 134
           WLSAA+++++TCL+GF+   G+ GE MK  LKT+ E+T NGL ++ E+++ V  + Q  G
Sbjct: 163 WLSAAISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIPG 222

Query: 135 LLQTGRQLRTEHSNEEKEEPSWVSDR-RGLLEATGA--NIKANVVVAKDGSGKYKTITEA 194
           L    R+L  E        PSWV  R R LL+A  A  ++K ++VVA+DGSG+YKTI EA
Sbjct: 223 L--NSRRLLAEGF------PSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEA 282

Query: 195 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 254
           L+ VP K NTTFV+++K G+Y+E V V+K M++++ IGDGP KT I+G+KN  DG TT++
Sbjct: 283 LQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYR 342

Query: 255 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 314
           +AT A VG+ FI K++ F+N+AG  K QAVA+RVQSD SIF+NCR DGYQDT Y H++RQ
Sbjct: 343 TATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQ 402

Query: 315 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 374
           F+RDCTISGT+DF+FG+AA V QNC +LVRKP P Q CPITA GR +  E TG V Q CT
Sbjct: 403 FFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCT 462

Query: 375 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 434
           I+ +PDY  V+ TSK+YLGRPWK+YSRT++M + I D +QP+GW PW GDF L TL+Y+E
Sbjct: 463 IAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSE 522

Query: 435 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALM 486
             N GPG+A  NRV W GIK ++ ++ + FTP+ +I GD WI   G+ YT  L+
Sbjct: 523 VQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

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BLAST of CmaCh06G015280 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.4e-140
Identity = 244/463 (52.70%), Postives = 331/463 (71.49%), Query Frame = 0


Query: 19  SPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSA 78
           S T+ +   DP A+ A+D C ELMDYAI +L  SF ++   F+ H+V++ L  LRIWLSA
Sbjct: 106 SQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSA 165

Query: 79  ALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLLQT 138
            ++++QTCLDGF+   G+ GE +K  LKT+ ++T NGL MV E+++ +  +  ++  + +
Sbjct: 166 TISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQM--QIPEMNS 225

Query: 139 GRQLRTEHSNEEKEEPSWVSDR-RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPL 198
            R L        +E PSW+  R R LL A  + +K ++VVA+DGSG+YKTI EAL  VP 
Sbjct: 226 RRLL-------SQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPK 285

Query: 199 KSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFKSATFAA 258
           K NTTFV+++KEG+Y+E V V++ MT+++ IGDGP KT I+GSK+  DG TT+K+AT A 
Sbjct: 286 KKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAI 345

Query: 259 VGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCT 318
           VG +FI K++ F+N+AG  K QAVA+RV +D SIFYNC+ DGYQDT Y H++RQFYRDCT
Sbjct: 346 VGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCT 405

Query: 319 ISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPD 378
           ISGT+DF+FG+AA V QNC +LVRKP   Q CPITA GR +  E TG VLQ CTI  +PD
Sbjct: 406 ISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPD 465

Query: 379 YYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGP 438
           Y  V+  SK+YLGRPWK+YSRT++M + I D + PEGW PW G+F LNTL+Y+E  N GP
Sbjct: 466 YLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGP 525

Query: 439 GAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISY 481
           GAA   RV W GIK+++ +E + FTP+ +I GD+WI   G+ Y
Sbjct: 526 GAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPY 558

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BLAST of CmaCh06G015280 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.0e-135
Identity = 240/465 (51.61%), Postives = 323/465 (69.46%), Query Frame = 0


Query: 24  QAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQ 83
           +AA D   + A++ C++LM+ A DDL    +   D F + ++ D++E+LR+WLS ++ YQ
Sbjct: 111 KAANDKDTKGALELCEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQ 170

Query: 84  QTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSL-----VTTLWQKLGLLQT 143
           QTC+D FE       + M+ + KTS+E+T+NGL M+  +++L     VT +   LG  + 
Sbjct: 171 QTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLG--KY 230

Query: 144 GRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLK 203
            R+L     + E   PSWV      L AT   +KANVVVA DGSG+YKTI EAL  VP  
Sbjct: 231 ARKL----LSAEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKA 290

Query: 204 SNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDG-TTTFKSATFAA 263
           +   FVIY+K+GVY E+V V KKMT+V  IGDGPTKTKITGS N   G   T+ +AT A 
Sbjct: 291 NQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAI 350

Query: 264 VGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCT 323
            G NF  K++ F+N+AG    QAVALRV +D+++FYNC++DGYQDT Y H++RQF+RDCT
Sbjct: 351 NGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCT 410

Query: 324 ISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPD 383
           +SGTVDFIFG+  VVLQNC I+VRKP   Q C ITAQGR +  E TG+VLQNC I+ +P 
Sbjct: 411 VSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPA 470

Query: 384 YYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGP 443
           Y PV+  +K+YLGRPWK++SRT++M + IDD+I P GWLPWNGDFALNTLYY E++N GP
Sbjct: 471 YIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGP 530

Query: 444 GAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTG 483
           G+ +  RVKW GIK+++ K+A+ FTP+ F+ G+ WI    + Y G
Sbjct: 531 GSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYMG 568

[Close]

BLAST of CmaCh06G015280 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 439.9 bits (1130), Expect = 3.8e-122
Identity = 216/463 (46.65%), Postives = 312/463 (67.39%), Query Frame = 0


Query: 24  QAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQ 83
           +A  +P A+ A + C++LM  AIDDL    +     F V ++  ++E+LR+WLS ++ +Q
Sbjct: 107 KADKNPEAKGAFELCEKLMIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQ 166

Query: 84  QTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLLQTGRQLR 143
           QTC+D F  +  +  + M  + KTS+E+++N L MV  +++L+       GL     +  
Sbjct: 167 QTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNS-NLTGLTGALAKYA 226

Query: 144 TEHSNEEKEEPSWVS-DRRGLLEATGAN---IKANVVVAKDGSGKYKTITEALKEVPLKS 203
            +  + E   P+WV  + R L+ A G     +KAN VVA+DG+G++KTIT+AL  VP  +
Sbjct: 227 RKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGN 286

Query: 204 NTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNV-VDGTTTFKSATFAAV 263
              F+I++KEG+Y+E+V V KKM +V  IGDGP KT ITGS N  +    TF +AT    
Sbjct: 287 KVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIE 346

Query: 264 GSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTI 323
           G +F  K++  +N+AG   GQAVALRV +D ++F++C++DG+QDT Y H++RQFYRDCT+
Sbjct: 347 GDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTV 406

Query: 324 SGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDY 383
           SGTVDFIFG+A  +LQNCKI+VRKP   Q C +TAQGR    E TG+VL  C I+ DP Y
Sbjct: 407 SGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAY 466

Query: 384 YPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPG 443
            P++  +K+YLGRPWK++SRT++M++ IDD+I P GWLPW+GDFAL TLYY E  N GPG
Sbjct: 467 IPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 526

Query: 444 AAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYT 482
           + +  RVKW GIK++T ++A+ +T   F+ GD+WI  T + YT
Sbjct: 527 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

[Close]

BLAST of CmaCh06G015280 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-111
Identity = 206/453 (45.47%), Postives = 288/453 (63.58%), Query Frame = 0


Query: 35  VDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDGFENVP 94
           ++ C+E++DYA+D +  S   + D FD H++++Y  +L++WL+  L++QQTCLDGF N  
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTL-DQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTT 60

Query: 95  GDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLLQTGRQLRTEHSNEEKEEP 154
              GE M  +LKTS E+++N + M+  V+ +       L    T +   +     +   P
Sbjct: 61  TKAGETMTKVLKTSMELSSNAIDMMDAVSRI-------LKGFDTSQYSVSRRLLSDDGIP 120

Query: 155 SWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYVKEGVYEE 214
           SWV+D    L A G N++ N VVA+DGSG++KT+T+ALK VP K+   FVI+VK GVY+E
Sbjct: 121 SWVNDGHRRLLA-GGNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKE 180

Query: 215 QVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDLWFDNSAG 274
            V V K+M YV VIGDGPTKTK TGS N  DG  T+ +ATF   G+NF+ KD+ F+N+AG
Sbjct: 181 TVNVAKEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAG 240

Query: 275 LYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFGNAAVVLQ 334
             K QAVALRV +D +IFYNC+MDG+QDT Y  + RQFYRDC+ISGT+DF+FG    V Q
Sbjct: 241 TGKHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQ 300

Query: 335 NCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKSYLGRPWK 394
           NCK++ R P   Q C +TA GR + +  + +V Q+   + +P    V     SYLGRPWK
Sbjct: 301 NCKLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPALTSVT-PKVSYLGRPWK 360

Query: 395 QYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKWKGIKQIT 454
           QYS+ V+M S ID +  PEG++PW G     T  Y E++N+GPGA    RVKW G+K +T
Sbjct: 361 QYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLT 420

Query: 455 AKEAINFTPSLFIH------GDSWIKNTGISYT 482
           +  A  + P  F         D+WI  +G+ Y+
Sbjct: 421 SNVAAEYYPGKFFEIVNATARDTWIVKSGVPYS 443

[Close]

BLAST of CmaCh06G015280 vs. ExPASy TrEMBL
Match: A0A6J1IFP3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472354 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.3e-278
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN
Sbjct: 86  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 145

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ
Sbjct: 146 LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 205

Query: 132 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 191
           KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA
Sbjct: 206 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 265

Query: 192 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 251
           LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK
Sbjct: 266 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 325

Query: 252 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 311
           SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ
Sbjct: 326 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 385

Query: 312 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 371
           FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT
Sbjct: 386 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 445

Query: 372 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 431
           ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE
Sbjct: 446 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 505

Query: 432 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI
Sbjct: 506 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 561

[Close]

BLAST of CmaCh06G015280 vs. ExPASy TrEMBL
Match: A0A6J1I9A9 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472265 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.3e-278
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN
Sbjct: 97  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 156

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ
Sbjct: 157 LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 216

Query: 132 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 191
           KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA
Sbjct: 217 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 276

Query: 192 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 251
           LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK
Sbjct: 277 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 336

Query: 252 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 311
           SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ
Sbjct: 337 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 396

Query: 312 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 371
           FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT
Sbjct: 397 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 456

Query: 372 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 431
           ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE
Sbjct: 457 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 516

Query: 432 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI
Sbjct: 517 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 572

[Close]

BLAST of CmaCh06G015280 vs. ExPASy TrEMBL
Match: A0A6J1FIT1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445855 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.1e-252
Identity = 432/460 (93.91%), Postives = 446/460 (96.96%), Query Frame = 0


Query: 30  LAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQQTCLDG 89
           +AQ AVDACDELMDYAIDDLLTSF+KITDSFDVHRVN YLENLRIWLSAALTYQ+TCLDG
Sbjct: 1   MAQKAVDACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDG 60

Query: 90  FENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLL-QTGRQLRTEHSN 149
           FENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLV+TLW+KLGLL QTGRQLRTE SN
Sbjct: 61  FENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVSTLWEKLGLLPQTGRQLRTEESN 120

Query: 150 EEKE-EPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYV 209
           E +E EPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYV
Sbjct: 121 EYQEGEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLKSNTTFVIYV 180

Query: 210 KEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFKSATFAAVGSNFIGKDL 269
           KEGVY+EQVMVDKKMTYVM+IGDGPTKTKIT +KNV+DGT TFKSATFAAVGSNFIGKDL
Sbjct: 181 KEGVYKEQVMVDKKMTYVMMIGDGPTKTKITANKNVIDGTPTFKSATFAAVGSNFIGKDL 240

Query: 270 WFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTISGTVDFIFG 329
           WF+NSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHA RQFYRDCTISGTVDFIFG
Sbjct: 241 WFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAKRQFYRDCTISGTVDFIFG 300

Query: 330 NAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKS 389
           NAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKS
Sbjct: 301 NAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDYYPVRHTSKS 360

Query: 390 YLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKW 449
           YLGRPWKQYSRTV+MQ QIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKW
Sbjct: 361 YLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPGAAKENRVKW 420

Query: 450 KGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           KG+KQITAK+AINFTPSLFI GD WIKNTGI YT ALMKI
Sbjct: 421 KGVKQITAKQAINFTPSLFIQGDDWIKNTGIPYTSALMKI 460

[Close]

BLAST of CmaCh06G015280 vs. ExPASy TrEMBL
Match: A0A6J1IFH7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472306 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.4e-251
Identity = 427/471 (90.66%), Postives = 449/471 (95.33%), Query Frame = 0


Query: 18  NSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLS 77
           +S TL+QAATDP+AQ AVDACDELMDYAIDDLLTSFNKITDSF  H++NDYLENLRIWLS
Sbjct: 96  SSSTLKQAATDPMAQKAVDACDELMDYAIDDLLTSFNKITDSFSFHKMNDYLENLRIWLS 155

Query: 78  AALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGL-L 137
           AALTYQ+TCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLV+TLWQKLGL  
Sbjct: 156 AALTYQETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVSTLWQKLGLPP 215

Query: 138 QTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVP 197
           QTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVP
Sbjct: 216 QTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVP 275

Query: 198 LKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFKSATFA 257
           LKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKIT SKNVVDGT TFKSATFA
Sbjct: 276 LKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITASKNVVDGTPTFKSATFA 335

Query: 258 AVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDC 317
           A+GSNFIGKDLWFDNSAGL+KGQAVALRVQSDMSIFYNCRMDGYQDT YPHANRQFYRDC
Sbjct: 336 AIGSNFIGKDLWFDNSAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPHANRQFYRDC 395

Query: 318 TISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDP 377
           TISGTVDFIFGNAA VLQNCKILVRKPKP Q C +TAQGRMESDEPTGIVLQNCTISSDP
Sbjct: 396 TISGTVDFIFGNAAAVLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQNCTISSDP 455

Query: 378 DYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRG 437
           ++YP+RHTSKSYLGRPWK+YSRTV+MQ QIDDLI P+GW+ W+ D  LNTLYYTEFDNRG
Sbjct: 456 EFYPIRHTSKSYLGRPWKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLNTLYYTEFDNRG 515

Query: 438 PGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           PGAAKENRVKWKGIKQITA +AINFTPSLFIHGDSWIK++GI YTG +MKI
Sbjct: 516 PGAAKENRVKWKGIKQITATQAINFTPSLFIHGDSWIKSSGIPYTGGMMKI 566

[Close]

BLAST of CmaCh06G015280 vs. ExPASy TrEMBL
Match: A0A6J1GCP0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452765 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 6.3e-245
Identity = 419/478 (87.66%), Postives = 448/478 (93.72%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTL+QA TDP+AQ AVDACDELMDYAIDDLLTSF+KIT+SF++H++NDYLEN
Sbjct: 86  LKAAIANSPTLKQATTDPMAQKAVDACDELMDYAIDDLLTSFDKITNSFNLHKMNDYLEN 145

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLK SKEMTANGLVMVGEVTSLV+TLW+
Sbjct: 146 LRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKMSKEMTANGLVMVGEVTSLVSTLWE 205

Query: 132 KLGL-LQTGRQLRTEHSNE-EKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTIT 191
           KLGL  QTGRQLRTE SNE ++EEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTIT
Sbjct: 206 KLGLPPQTGRQLRTEESNEYQEEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTIT 265

Query: 192 EALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTT 251
           EALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVM+IGDGPTKTKIT +KNV DGT T
Sbjct: 266 EALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITANKNVQDGTPT 325

Query: 252 FKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAN 311
           FKSATFAA+GSNFIGKDLWFDNSAGL+KGQAVALRVQSDMSIFYNCRMDGYQDT YP   
Sbjct: 326 FKSATFAAIGSNFIGKDLWFDNSAGLHKGQAVALRVQSDMSIFYNCRMDGYQDTLYPQTK 385

Query: 312 RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQN 371
           RQFYRDCTISGTVDFIFGNAA VLQNCKILVRKPKP Q C +TAQGRMESDEPTGIVLQN
Sbjct: 386 RQFYRDCTISGTVDFIFGNAAAVLQNCKILVRKPKPNQACFVTAQGRMESDEPTGIVLQN 445

Query: 372 CTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYY 431
           C ISSDP++YP+RHTSKSYLGRPWK+YSRTV+MQ QIDDLI P+GW+ W+ D  L TLYY
Sbjct: 446 CVISSDPEFYPIRHTSKSYLGRPWKKYSRTVIMQCQIDDLIHPDGWMRWSDDSTLKTLYY 505

Query: 432 TEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           TEFDNRGPGAAKENRVKWKGIKQITAK+AINFTPSLFI GD WIKN+GI YTG +MKI
Sbjct: 506 TEFDNRGPGAAKENRVKWKGIKQITAKQAINFTPSLFIQGDDWIKNSGIPYTGGMMKI 563

[Close]

BLAST of CmaCh06G015280 vs. NCBI nr
Match: XP_022973799.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 966.8 bits (2498), Expect = 6.8e-278
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN
Sbjct: 86  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 145

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ
Sbjct: 146 LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 205

Query: 132 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 191
           KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA
Sbjct: 206 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 265

Query: 192 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 251
           LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK
Sbjct: 266 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 325

Query: 252 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 311
           SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ
Sbjct: 326 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 385

Query: 312 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 371
           FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT
Sbjct: 386 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 445

Query: 372 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 431
           ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE
Sbjct: 446 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 505

Query: 432 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI
Sbjct: 506 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 561

[Close]

BLAST of CmaCh06G015280 vs. NCBI nr
Match: XP_022973681.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 966.8 bits (2498), Expect = 6.8e-278
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN
Sbjct: 97  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 156

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ
Sbjct: 157 LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 216

Query: 132 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 191
           KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA
Sbjct: 217 KLGLLQTGRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEA 276

Query: 192 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 251
           LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK
Sbjct: 277 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 336

Query: 252 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 311
           SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ
Sbjct: 337 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 396

Query: 312 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 371
           FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT
Sbjct: 397 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 456

Query: 372 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 431
           ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE
Sbjct: 457 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 516

Query: 432 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI
Sbjct: 517 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 572

[Close]

BLAST of CmaCh06G015280 vs. NCBI nr
Match: KAG6597545.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 908.3 bits (2346), Expect = 2.9e-260
Identity = 448/478 (93.72%), Postives = 463/478 (96.86%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTL+QAATDP+AQ AVDACDELMDYAIDDLLTSF+KITDSFDVHRVN YLEN
Sbjct: 86  LKAAIANSPTLKQAATDPMAQKAVDACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLEN 145

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLKTS+EMTANGLVMVGEVTSLV+TLW+
Sbjct: 146 LRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKTSREMTANGLVMVGEVTSLVSTLWE 205

Query: 132 KLGLL-QTGRQLRTEHSNEEKE-EPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTIT 191
           KLGLL QTGRQLRTE SNE +E EPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTIT
Sbjct: 206 KLGLLPQTGRQLRTEESNEYQEGEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTIT 265

Query: 192 EALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTT 251
           EALKEVPLKSNTTFVIYVKEGVYEEQV+VDKKMTYVMVIGDGPTKTKITG K+V DGT T
Sbjct: 266 EALKEVPLKSNTTFVIYVKEGVYEEQVLVDKKMTYVMVIGDGPTKTKITGRKSVEDGTPT 325

Query: 252 FKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAN 311
           FKSATFAAVGSNFIGKDLWF+NSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHA 
Sbjct: 326 FKSATFAAVGSNFIGKDLWFENSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHAK 385

Query: 312 RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQN 371
           RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQN
Sbjct: 386 RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQN 445

Query: 372 CTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYY 431
           CTISS+PDYYPVRHTSKSYLGRPWKQYSRTV+MQ QIDDLIQPEGWLPWNGDFALNTLYY
Sbjct: 446 CTISSEPDYYPVRHTSKSYLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLYY 505

Query: 432 TEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           TEFDNRGPGAAKENRVKWKG+KQITAK+AINFTPSLFI GD WIKNTGI YT ALMKI
Sbjct: 506 TEFDNRGPGAAKENRVKWKGVKQITAKQAINFTPSLFIQGDDWIKNTGIPYTSALMKI 563

[Close]

BLAST of CmaCh06G015280 vs. NCBI nr
Match: XP_023539372.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.1 bits (2330), Expect = 2.1e-258
Identity = 444/479 (92.69%), Postives = 459/479 (95.82%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LK AIANSPTL+QAATDP+AQ AVDACDELMDYAIDDLLTSF+KITDSFDVHRVNDYLEN
Sbjct: 86  LKVAIANSPTLKQAATDPMAQKAVDACDELMDYAIDDLLTSFDKITDSFDVHRVNDYLEN 145

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLV+TLW+
Sbjct: 146 LRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVSTLWE 205

Query: 132 KLGL-LQTGRQLRTEHSNEE--KEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTI 191
           KLGL  QTGRQLRTE SNEE  +EEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTI
Sbjct: 206 KLGLPPQTGRQLRTEESNEEFQEEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTI 265

Query: 192 TEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTT 251
           TEALKEVPLKSNTTFVI+VKEGVYEEQVMVDKKMTYVM+IGDGPTKTKIT SKNV DGT 
Sbjct: 266 TEALKEVPLKSNTTFVIHVKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITASKNVADGTP 325

Query: 252 TFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHA 311
           TFKSATFAA+GSNFIGKDLWFDNSAG+YKGQAVALRVQSDMSIFYNCRMDGYQDTFYP  
Sbjct: 326 TFKSATFAAIGSNFIGKDLWFDNSAGIYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPQT 385

Query: 312 NRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQ 371
            RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQ
Sbjct: 386 KRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQ 445

Query: 372 NCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLY 431
           NC ISSDPDYYPVRHTSKSYLGRPWKQYSRTV+MQ QIDDLIQPEGWLPWNGDFALNTLY
Sbjct: 446 NCIISSDPDYYPVRHTSKSYLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLY 505

Query: 432 YTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           YTEF+NRGPGAAKENRVKWKG+KQITAK+AINFTPSLFI GD WIK TGI YT ALMKI
Sbjct: 506 YTEFENRGPGAAKENRVKWKGVKQITAKQAINFTPSLFIQGDDWIKKTGIPYTSALMKI 564

[Close]

BLAST of CmaCh06G015280 vs. NCBI nr
Match: XP_023539368.1 (pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539373.1 pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539374.1 pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539376.1 pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.4 bits (2328), Expect = 3.5e-258
Identity = 443/479 (92.48%), Postives = 460/479 (96.03%), Query Frame = 0


Query: 12  LKAAIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLEN 71
           LKAAIANSPTL+QAATDP+AQ AVDACDELMDYAIDDLLTSF+KITDSFDVH++NDYLEN
Sbjct: 86  LKAAIANSPTLKQAATDPMAQKAVDACDELMDYAIDDLLTSFDKITDSFDVHKMNDYLEN 145

Query: 72  LRIWLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQ 131
           LRIWLSAALTYQ+TCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLV+TLW+
Sbjct: 146 LRIWLSAALTYQETCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVSTLWE 205

Query: 132 KLGL-LQTGRQLRTEHSNEE--KEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTI 191
           KLGL  QTGRQLRTE SNEE  +EEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTI
Sbjct: 206 KLGLPPQTGRQLRTEESNEEFQEEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTI 265

Query: 192 TEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTT 251
           TEALKEVPLKSNTTFVI+VKEGVYEEQVMVDKKMTYVM+IGDGPTKTKIT SKNV DGT 
Sbjct: 266 TEALKEVPLKSNTTFVIHVKEGVYEEQVMVDKKMTYVMMIGDGPTKTKITASKNVADGTP 325

Query: 252 TFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHA 311
           TFKSATFAA+GSNFIGKDLWFDNSAG+YKGQAVALRVQSDMSIFYNCRMDGYQDTFYP  
Sbjct: 326 TFKSATFAAIGSNFIGKDLWFDNSAGIYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPQT 385

Query: 312 NRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQ 371
            RQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQ
Sbjct: 386 KRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQ 445

Query: 372 NCTISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLY 431
           NC ISSDPDYYPVRHTSKSYLGRPWKQYSRTV+MQ QIDDLIQPEGWLPWNGDFALNTLY
Sbjct: 446 NCIISSDPDYYPVRHTSKSYLGRPWKQYSRTVIMQCQIDDLIQPEGWLPWNGDFALNTLY 505

Query: 432 YTEFDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALMKI 488
           YTEF+NRGPGAAKENRVKWKG+KQITAK+AINFTPSLFI GD WIK TGI YT ALMKI
Sbjct: 506 YTEFENRGPGAAKENRVKWKGVKQITAKQAINFTPSLFIQGDDWIKKTGIPYTSALMKI 564

[Close]

BLAST of CmaCh06G015280 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 507.3 bits (1305), Expect = 1.4e-143
Identity = 249/474 (52.53%), Postives = 338/474 (71.31%), Query Frame = 0


Query: 15  AIANSPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRI 74
           A   S T+ +   D   + A+D C ELMDYA+D+L  SF ++   F+ H +++ L NLRI
Sbjct: 103 AAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRI 162

Query: 75  WLSAALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLG 134
           WLSAA+++++TCL+GF+   G+ GE MK  LKT+ E+T NGL ++ E+++ V  + Q  G
Sbjct: 163 WLSAAISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIPG 222

Query: 135 LLQTGRQLRTEHSNEEKEEPSWVSDR-RGLLEATGA--NIKANVVVAKDGSGKYKTITEA 194
           L    R+L  E        PSWV  R R LL+A  A  ++K ++VVA+DGSG+YKTI EA
Sbjct: 223 L--NSRRLLAEGF------PSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEA 282

Query: 195 LKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFK 254
           L+ VP K NTTFV+++K G+Y+E V V+K M++++ IGDGP KT I+G+KN  DG TT++
Sbjct: 283 LQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYR 342

Query: 255 SATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQ 314
           +AT A VG+ FI K++ F+N+AG  K QAVA+RVQSD SIF+NCR DGYQDT Y H++RQ
Sbjct: 343 TATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQ 402

Query: 315 FYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCT 374
           F+RDCTISGT+DF+FG+AA V QNC +LVRKP P Q CPITA GR +  E TG V Q CT
Sbjct: 403 FFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCT 462

Query: 375 ISSDPDYYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTE 434
           I+ +PDY  V+ TSK+YLGRPWK+YSRT++M + I D +QP+GW PW GDF L TL+Y+E
Sbjct: 463 IAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSE 522

Query: 435 FDNRGPGAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTGALM 486
             N GPG+A  NRV W GIK ++ ++ + FTP+ +I GD WI   G+ YT  L+
Sbjct: 523 VQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of CmaCh06G015280 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 501.1 bits (1289), Expect = 9.8e-142
Identity = 244/463 (52.70%), Postives = 331/463 (71.49%), Query Frame = 0


Query: 19  SPTLRQAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSA 78
           S T+ +   DP A+ A+D C ELMDYAI +L  SF ++   F+ H+V++ L  LRIWLSA
Sbjct: 106 SQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSA 165

Query: 79  ALTYQQTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLLQT 138
            ++++QTCLDGF+   G+ GE +K  LKT+ ++T NGL MV E+++ +  +  ++  + +
Sbjct: 166 TISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQM--QIPEMNS 225

Query: 139 GRQLRTEHSNEEKEEPSWVSDR-RGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPL 198
            R L        +E PSW+  R R LL A  + +K ++VVA+DGSG+YKTI EAL  VP 
Sbjct: 226 RRLL-------SQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPK 285

Query: 199 KSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDGTTTFKSATFAA 258
           K NTTFV+++KEG+Y+E V V++ MT+++ IGDGP KT I+GSK+  DG TT+K+AT A 
Sbjct: 286 KKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAI 345

Query: 259 VGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCT 318
           VG +FI K++ F+N+AG  K QAVA+RV +D SIFYNC+ DGYQDT Y H++RQFYRDCT
Sbjct: 346 VGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCT 405

Query: 319 ISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPD 378
           ISGT+DF+FG+AA V QNC +LVRKP   Q CPITA GR +  E TG VLQ CTI  +PD
Sbjct: 406 ISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPD 465

Query: 379 YYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGP 438
           Y  V+  SK+YLGRPWK+YSRT++M + I D + PEGW PW G+F LNTL+Y+E  N GP
Sbjct: 466 YLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGP 525

Query: 439 GAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISY 481
           GAA   RV W GIK+++ +E + FTP+ +I GD+WI   G+ Y
Sbjct: 526 GAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPY 558

[Close]

BLAST of CmaCh06G015280 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.0 bits (1247), Expect = 7.3e-137
Identity = 240/465 (51.61%), Postives = 323/465 (69.46%), Query Frame = 0


Query: 24  QAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQ 83
           +AA D   + A++ C++LM+ A DDL    +   D F + ++ D++E+LR+WLS ++ YQ
Sbjct: 111 KAANDKDTKGALELCEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQ 170

Query: 84  QTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSL-----VTTLWQKLGLLQT 143
           QTC+D FE       + M+ + KTS+E+T+NGL M+  +++L     VT +   LG  + 
Sbjct: 171 QTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLG--KY 230

Query: 144 GRQLRTEHSNEEKEEPSWVSDRRGLLEATGANIKANVVVAKDGSGKYKTITEALKEVPLK 203
            R+L     + E   PSWV      L AT   +KANVVVA DGSG+YKTI EAL  VP  
Sbjct: 231 ARKL----LSAEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKA 290

Query: 204 SNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNVVDG-TTTFKSATFAA 263
           +   FVIY+K+GVY E+V V KKMT+V  IGDGPTKTKITGS N   G   T+ +AT A 
Sbjct: 291 NQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAI 350

Query: 264 VGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCT 323
            G NF  K++ F+N+AG    QAVALRV +D+++FYNC++DGYQDT Y H++RQF+RDCT
Sbjct: 351 NGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCT 410

Query: 324 ISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPD 383
           +SGTVDFIFG+  VVLQNC I+VRKP   Q C ITAQGR +  E TG+VLQNC I+ +P 
Sbjct: 411 VSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPA 470

Query: 384 YYPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGP 443
           Y PV+  +K+YLGRPWK++SRT++M + IDD+I P GWLPWNGDFALNTLYY E++N GP
Sbjct: 471 YIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGP 530

Query: 444 GAAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYTG 483
           G+ +  RVKW GIK+++ K+A+ FTP+ F+ G+ WI    + Y G
Sbjct: 531 GSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYMG 568

[Close]

BLAST of CmaCh06G015280 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.9 bits (1130), Expect = 2.7e-123
Identity = 216/463 (46.65%), Postives = 312/463 (67.39%), Query Frame = 0


Query: 24  QAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQ 83
           +A  +P A+ A + C++LM  AIDDL    +     F V ++  ++E+LR+WLS ++ +Q
Sbjct: 107 KADKNPEAKGAFELCEKLMIDAIDDLKKCMD---HGFSVDQIEVFVEDLRVWLSGSIAFQ 166

Query: 84  QTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTLWQKLGLLQTGRQLR 143
           QTC+D F  +  +  + M  + KTS+E+++N L MV  +++L+       GL     +  
Sbjct: 167 QTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNS-NLTGLTGALAKYA 226

Query: 144 TEHSNEEKEEPSWVS-DRRGLLEATGAN---IKANVVVAKDGSGKYKTITEALKEVPLKS 203
            +  + E   P+WV  + R L+ A G     +KAN VVA+DG+G++KTIT+AL  VP  +
Sbjct: 227 RKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGN 286

Query: 204 NTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGSKNV-VDGTTTFKSATFAAV 263
              F+I++KEG+Y+E+V V KKM +V  IGDGP KT ITGS N  +    TF +AT    
Sbjct: 287 KVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIE 346

Query: 264 GSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQDTFYPHANRQFYRDCTI 323
           G +F  K++  +N+AG   GQAVALRV +D ++F++C++DG+QDT Y H++RQFYRDCT+
Sbjct: 347 GDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTV 406

Query: 324 SGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEPTGIVLQNCTISSDPDY 383
           SGTVDFIFG+A  +LQNCKI+VRKP   Q C +TAQGR    E TG+VL  C I+ DP Y
Sbjct: 407 SGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAY 466

Query: 384 YPVRHTSKSYLGRPWKQYSRTVVMQSQIDDLIQPEGWLPWNGDFALNTLYYTEFDNRGPG 443
            P++  +K+YLGRPWK++SRT++M++ IDD+I P GWLPW+GDFAL TLYY E  N GPG
Sbjct: 467 IPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 526

Query: 444 AAKENRVKWKGIKQITAKEAINFTPSLFIHGDSWIKNTGISYT 482
           + +  RVKW GIK++T ++A+ +T   F+ GD+WI  T + YT
Sbjct: 527 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYT 565

[Close]

BLAST of CmaCh06G015280 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 395.6 bits (1015), Expect = 5.8e-110
Identity = 222/598 (37.12%), Postives = 317/598 (53.01%), Query Frame = 0


Query: 24  QAATDPLAQNAVDACDELMDYAIDDLLTSFNKITDSFDVHRVNDYLENLRIWLSAALTYQ 83
           +A  D   + A++ C ELMD AIDDL  + +K    F   R++D++E+L +WLS ++TYQ
Sbjct: 102 RADADMHTREALNTCRELMDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQ 161

Query: 84  QTCLDGFENVPGDTGEKMKNLLKTSKEMTANGLVMVGEVTSLVTTL----------WQKL 143
           QTC+DGFE +  +    M+ +++  + +T+NGL +   +  L+               +L
Sbjct: 162 QTCIDGFEGIDSEAAVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRL 221

Query: 144 GLLQTGR----------------------------------------------------- 203
           G+L +G                                                      
Sbjct: 222 GILGSGSSRDESVGSSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDS 281

Query: 204 ------------------------------------------------------------ 263
                                                                       
Sbjct: 282 SKNQQMESSEDTPKKSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRP 341

Query: 264 --------------QLRTEHSNEEKEEPSWVSDR-RGLLEATGAN--IKANVVVAKDGSG 323
                          L+  H +EE E P WV+   R LL     N  IKANVVVAKDGSG
Sbjct: 342 LDPLRKLNPLNKLDSLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSG 401

Query: 324 KYKTITEALKEVPLKSNTTFVIYVKEGVYEEQVMVDKKMTYVMVIGDGPTKTKITGS-KN 383
           K KTI +AL  VP+K+   FVI++KEGVY+E+V V KKM +VM +GDGPTKT ITG    
Sbjct: 402 KCKTIAQALAMVPMKNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAF 461

Query: 384 VVDGTTTFKSATFAAVGSNFIGKDLWFDNSAGLYKGQAVALRVQSDMSIFYNCRMDGYQD 443
           + D   T+++A+ A  G  F+ KD+ F+N+AG  + QAVALRV +D ++F+NC M+GYQD
Sbjct: 462 LPDQVGTYRTASVAVNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQD 521

Query: 444 TFYPHANRQFYRDCTISGTVDFIFGNAAVVLQNCKILVRKPKPKQPCPITAQGRMESDEP 481
           T Y H +RQFYR+C +SGT+DF+FG+A  V QNC+ ++R+P   Q C +TAQGR +  E 
Sbjct: 522 TLYVHTHRQFYRNCRVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRET 581

The following BLAST results are available for this feature:

BLAST of CmaCh06G015280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GX86	1.9e-142	52.53	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q3E8Z8	1.4e-140	52.70	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	1.0e-135	51.61	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	3.8e-122	46.65	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
Q42920	1.4e-111	45.47	Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1	[more]

BLAST of CmaCh06G015280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IFP3	3.3e-278	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472354 PE=3 SV=1	[more]
A0A6J1I9A9	3.3e-278	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472265 PE=3 SV=1	[more]
A0A6J1FIT1	3.1e-252	93.91	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445855 PE=3 SV=1	[more]
A0A6J1IFH7	3.4e-251	90.66	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472306 PE=3 SV=1	[more]
A0A6J1GCP0	6.3e-245	87.66	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452765 PE=3 SV=1	[more]

BLAST of CmaCh06G015280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022973799.1	6.8e-278	100.00	pectinesterase-like [Cucurbita maxima]	[more]
XP_022973681.1	6.8e-278	100.00	pectinesterase-like [Cucurbita maxima]	[more]
KAG6597545.1	2.9e-260	93.72	putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosp...	[more]
XP_023539372.1	2.1e-258	92.69	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023539368.1	3.5e-258	92.48	pectinesterase-like [Cucurbita pepo subsp. pepo] >XP_023539373.1 pectinesterase-...	[more]

BLAST of CmaCh06G015280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05610.1	1.4e-143	52.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	9.8e-142	52.70	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	7.3e-137	51.61	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	2.7e-123	46.65	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	5.8e-110	37.12	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 3..118 e-value: 1.4E-6 score: 37.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 22..116 e-value: 5.3E-15 score: 56.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 15..119 e-value: 2.6E-13 score: 47.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 168..484 e-value: 3.7E-118 score: 396.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 5..122 e-value: 1.0E-18 score: 69.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 13..121
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 174..470 e-value: 3.9E-141 score: 469.0
None	No IPR available	PANTHER	PTHR31707:SF337	PECTINESTERASE	coord: 11..483
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 11..483
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 12..124 e-value: 1.04413E-26 score: 103.672
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 318..327
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 171..481

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G015280.1	CmaCh06G015280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity