CmaCh06G012110 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G012110
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RING-type E3 ubiquitin transferase
Location	Cma_Chr06: 8078042 .. 8079519 (-)
RNA-Seq Expression	CmaCh06G012110
Synteny	CmaCh06G012110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTTTTGCAGATCAAAACCTTCTGCTTCGCTTCATTCATCAATGGATTCCGATTTTCCTCCGCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGATCACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTTCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCCATGATTGAAACGAGCGATGAGATGAAATCGGGATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATCGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGTAGTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGATCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATTGAATGA

mRNA sequence

ATGAAGCTTTTTGCAGATCAAAACCTTCTGCTTCGCTTCATTCATCAATGGATTCCGATTTTCCTCCGCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGATCACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTTCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCCATGATTGAAACGAGCGATGAGATGAAATCGGGATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATCGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTAGGGGATCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATTGAATGA

Coding sequence (CDS)

ATGAAGCTTTTTGCAGATCAAAACCTTCTGCTTCGCTTCATTCATCAATGGATTCCGATTTTCCTCCGCTTTTCGGATGCCCGATTTCCATGGAGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGGCTCATGGAGGATCCCGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTCTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACCCTCGCCGCTGGCGACTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGATCACTGATTCTTCTGCGTCGCCGGCGACGATCGAGGAAGTTCGATCGCTGCTTGCGACTGTTGAGTCTTCTCCATTCAAGGTGAGTTCGTTGAGGAAATTGAGATCCATGATTGAAACGAGCGATGAGATGAAATCGGGATTTGTTCGATTGAATGGGATTGGAATTGTTGTGAATGTTGTCGTTCAAATTCTCATTGATTGTTCTGATTTTTGGACGTTTGAAGCTTGTGAAGAGGCTGTGGGAGTGTTGTCTCAGCTTCCGATTTCGAACGAAGACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCATCGCTATTGTTCTTCAAAGGGGAAGTGCTGAAGGCCGTTTTTACGCTGTGAAAATCCTAAGAAATATTGCTAAAAACACCAATTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATCGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTGTCAAAGAGAATTCTCCACAAAGGCAAGCTCGTGCGCATTGGATGTAATGATGGAAATTTTAGCATTTCGAGTGAAGGCGATCGAGGCCGGTGCTGTGTGCATCCTCATCGAGCTCTTACCGGAGTCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTAGGGGATCAGGTCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCAGTGACTAAGAAGATTTTGAATGTTTCAAACGTAGCAACAAAAATGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAATGAAAGGGTTGTGGAAGAGATGATGGCTTGTGGGGTAGTGAAGAAGCTGATGATGATATTGCACATGGACGGCGGCGGCGGTGGCCGGAGTTCGACGAAGGAGAAGGCGATCAAGATTTTGAAGATGCATGGGAGCTCATGGAGGCGTAGCCCTTGTTTTCCAAGTGAGTTGAAGAATTATTTGATGTTTGTGAATATTGAATGA

Protein sequence

MKLFADQNLLLRFIHQWIPIFLRFSDARFPWSSWRIPSPSPPASPTREKASKNGLMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKITDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE

Homology

[Close]

BLAST of CmaCh06G012110 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.9e-41
Identity = 130/434 (29.95%), Postives = 211/434 (48.62%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R SIEKWLF     +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 24  IMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD--LTPNHTLRRLIQSWCT 83

Query: 115 TDSS---------ASPATIEEVRSLLA-TVESSPFKVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+  +  S   +V  L++LR ++  +   K      
Sbjct: 84  LNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSENATNKRCLEAA 143

Query: 175 NGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREE--LMKSI 234
                + N+V       +D       +EA+ +L  L  S E  L + ++ +++  ++KS+
Sbjct: 144 GVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVLKNLLNNKKDNNIVKSL 203

Query: 235 AIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC 294
             ++QRG  E R YA  +L+NI +  +      L+    + F  +++++ D  S KA+  
Sbjct: 204 TKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEVVQILDDRISQKATKA 263

Query: 295 ALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRAL-TGVRS--IE 354
           A+ +++ I                 C  G               + H+A+  GV S  IE
Sbjct: 264 AMHILVNI-----------------CPWGR--------------NRHKAVEAGVISVIIE 323

Query: 355 MRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVA 414
           +  + + ++E RG      E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A
Sbjct: 324 LLMDESFTSERRGP-----EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTA 383

Query: 415 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMH 474
           +   V++LL +  F     ++ EM+  GVV KL ++L +  GG     TKEKA ++LK+H
Sbjct: 384 SDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGG----KTKEKAKELLKLH 405

[Close]

BLAST of CmaCh06G012110 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.8e-39
Identity = 131/456 (28.73%), Postives = 210/456 (46.05%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 19  IMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LTPNHTLRRLIQSWCT 78

Query: 115 TDSS---------ASPATIEEVRSLLATVESSPF-KVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+    SS   +V  L++LR ++  +   K      
Sbjct: 79  LNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVSENTTNKRCLEAA 138

Query: 175 NGIGIVVNVVVQIL-------IDCSDFWTFEAC----------------EEAVGVLSQLP 234
                + N+V   +          S     + C                +EA+ VL  L 
Sbjct: 139 EVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSLMDEALSVLYHLD 198

Query: 235 ISNED-KLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQG 294
            S    K      K   L+K++  ++QRG  E R YA  +L+ + +  +     +LE   
Sbjct: 199 TSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVADPMQIILLER-- 258

Query: 295 IDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGD 354
            + F  +++++ D+ S KA+  A+ +++                 I C  G         
Sbjct: 259 -ELFGEVIQILHDQISHKATRSAMQILV-----------------ITCPWGR-------- 318

Query: 355 RGRCCVHPHRALTG---VRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLA 414
                 + H+A+ G      IE+  +   S+E R S     E  + +L  LC+CAEGR  
Sbjct: 319 ------NRHKAVEGGTISMIIELLMDDTFSSERRNS-----EMAMVVLDMLCQCAEGRAE 378

Query: 415 LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILH 474
            + HG  IA V+KKIL VS + ++  V++LL +  F     +++EM+  GVV KL ++L 
Sbjct: 379 FLNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ 429

[Close]

BLAST of CmaCh06G012110 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 8.3e-29
Identity = 120/445 (26.97%), Postives = 207/445 (46.52%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAW-- 114
           +M+DPVT  +G+TY+R++I KWL      +CP T Q L   D  +TPN  L+RLI  W  
Sbjct: 22  IMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL-DSDLTPNHMLRRLIQHWCV 81

Query: 115 --------KITDSSASPA---TIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGF 174
                   +I+     P     +EE+++L    + +  +  +L+KL  +    +  +   
Sbjct: 82  ENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEVLAMDGNNRRLMC 141

Query: 175 VRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPI-SNEDKLFDEMSKREELMK 234
                  +++ VV     D       +  +E++ +L  + I SN+ K    + + + +M+
Sbjct: 142 ECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKTI--LMENDRVME 201

Query: 235 SIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKAS 294
           S+  VL +     + Y + +LRN+   T +    ++E    + FK ++  + D  ++  +
Sbjct: 202 SLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPEIFKGIIGFLKDVVNS-VN 261

Query: 295 SCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEM 354
             +  V   + + S+  L K +  ++   D +  I          +    + +  RS+ +
Sbjct: 262 RTSPTVRETVQSSSRPSLGKTEPSKL---DHSLVIKQAVTAALMILLETSSWSRNRSLLV 321

Query: 355 RANAAD---SAEFRGSGRSK-CERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 414
              A       E   +G  +  E ML +L  LC CA GR  ++ H  GIA VTK++L VS
Sbjct: 322 DLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLLRVS 381

Query: 415 NVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKIL 474
             A    + IL  +S F P   VVEEM+  G V+KL  +L MD G     + KEKA +IL
Sbjct: 382 PAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG----LNLKEKAKEIL 441

Query: 475 KMHGSSWRRSPCFPSELKNYLMFVN 482
           K H   W++ PC    L   L+ ++
Sbjct: 442 KDHFDEWKKFPCIDITLLTKLLSIS 450

[Close]

BLAST of CmaCh06G012110 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.4e-23
Identity = 118/428 (27.57%), Postives = 188/428 (43.93%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPVT+ TG TY+R SIE W+   NN TCP T   L+  DF + PN  L+RLI  W +
Sbjct: 26  LMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLIPNHTLRRLIQEWCV 85

Query: 115 TDSS---------ASPATIEEVRSLLA-------TVESSPFKVSSLRKLRSMIETSDEMK 174
            + S           PA    VR+LL+       T  S   + ++LR+LR     SD+ +
Sbjct: 86  ANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRGFARDSDKNR 145

Query: 175 SGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 234
                 N   I++ ++       S+  + E   E++ +L  LPI+ E   F  +S     
Sbjct: 146 VLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-EPNQFVSISSDPGR 205

Query: 235 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFN--WGSVLEEQGIDFFKPLLELVSDEFS 294
           ++ +  +L   S E R  A  ++  ++  T      GS+   + +  F+ +L+L+ +  S
Sbjct: 206 VEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--FEGVLDLLRNPIS 265

Query: 295 TKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT-GV 354
           ++                       + ++IG        S +  R       H A+T G 
Sbjct: 266 SR-----------------------RALKIGIKTLFALCSVKSTR-------HIAITAGA 325

Query: 355 RSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNV 414
             I +   AAD        R   ER L  ++ LC   EG  A  EH + +  + K IL V
Sbjct: 326 PEILIDRLAAD------FDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRV 385

Query: 415 SNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKI 464
           S+ AT+     LL + +    ER  EE    GVV +L++++  +         K+KA K+
Sbjct: 386 SDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE----CTERAKKKAQKL 400

[Close]

BLAST of CmaCh06G012110 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.9e-20
Identity = 117/441 (26.53%), Postives = 193/441 (43.76%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPV ISTG+TY+R SIE W+    NKTCP T   L   D +  PN  ++++I  W +
Sbjct: 43  LMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQI--PNHTIRKMIQGWCV 102

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFV--RLNGIG---- 174
              S     I   R  L   E   +++S  RKL S     D  K G +  ++  +G    
Sbjct: 103 EKGSPLIQRIPTPRVPLMPCE--VYEIS--RKLSSATRRGDYEKCGVIIEKIKKLGDESE 162

Query: 175 -----IVVNVVVQILIDCSDFWTFEACEEAVGVLSQL--------PISNE--DKLFDEMS 234
                +  N V  +L DC D   F   E+   +L+++        PI  E   KL    S
Sbjct: 163 KNRKCVNENSVGWVLCDCFD--KFSGDEKLTFMLNEILSLLTWMFPIGLEGISKLASATS 222

Query: 235 KREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSD 294
                 + +A +L+      R  A  I++ I         S   E G+   + L++L+ D
Sbjct: 223 -----FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV--AEALVKLIRD 282

Query: 295 EFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT 354
             S+ ++  +L      +A+ + +L K ++       G  SI                  
Sbjct: 283 SVSSSSTKSSL------IAIYQMVLQKPEIASEFLEIGLVSI------------------ 342

Query: 355 GVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKIL 414
              ++EM  +A +S          CE+ L +L ++CE   GR  + ++ + +  + KKI 
Sbjct: 343 ---TVEMIVDAENSV---------CEKALAVLDAICETEHGREEVRKNALVMPLLVKKIA 402

Query: 415 NVSNVATK--MGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEK 472
            VS +AT+  M + + LW +    N   VE+ +  G  +K++++L +  G      TKEK
Sbjct: 403 KVSELATRSSMSMILKLWKTG---NTVAVEDAVRLGAFQKVLLVLQVGYG----EETKEK 424

[Close]

BLAST of CmaCh06G012110 vs. ExPASy TrEMBL
Match: A0A6J1IKT9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 PE=4 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.7e-200
Identity = 376/429 (87.65%), Postives = 379/429 (88.34%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           K                                     + V++IE  A          SG
Sbjct: 257 KS------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELK 474
           PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELK
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELK 409

Query: 475 NYLMFVNIE 484
           NYLMFVNIE
Sbjct: 437 NYLMFVNIE 409

[Close]

BLAST of CmaCh06G012110 vs. ExPASy TrEMBL
Match: A0A6J1H765 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.7e-197
Identity = 374/433 (86.37%), Postives = 377/433 (87.07%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL LKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLTLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKS FVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC LDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCTLDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           KR                                    + V++IE  A          SG
Sbjct: 257 KR------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMD----GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 474
           PNERVVEEMMACGVVKKLMMILHMD    GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 413

Query: 475 SELKNYLMFVNIE 484
           SELKNYLMFVNIE
Sbjct: 437 SELKNYLMFVNIE 413

[Close]

BLAST of CmaCh06G012110 vs. ExPASy TrEMBL
Match: A0A6J1H5I9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 2.2e-189
Identity = 361/420 (85.95%), Postives = 364/420 (86.67%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL LKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLTLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKS FVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC LDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCTLDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           KR                                    + V++IE  A          SG
Sbjct: 257 KR------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMD----GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 471
           PNERVVEEMMACGVVKKLMMILHMD    GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 400

[Close]

BLAST of CmaCh06G012110 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.8e-172
Identity = 330/435 (75.86%), Postives = 356/435 (81.84%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--DFVITPNLNLKRLILAW 114
           LMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTLAAG  DFVITPNLNLKRLILAW
Sbjct: 17  LMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITPNLNLKRLILAW 76

Query: 115 KITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVV 174
           KI DS+ +PAT +EEV SLLATVESSPFKVSSLRKLRSM+E +D MKS F+RL+GIGIVV
Sbjct: 77  KINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMNDGMKSEFIRLSGIGIVV 136

Query: 175 NVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSA 234
           NVV+QILIDCSDF T + CEEA+GVL Q PISNEDKLFDEM KREELMKSIA+V+QRGSA
Sbjct: 137 NVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKREELMKSIAVVVQRGSA 196

Query: 235 EGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEIL 294
           EGRFYAVKILRNIAKNTN+NWGS++EEQGIDFFKPLLELVSDEF TKASSCALDVM+EIL
Sbjct: 197 EGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEIL 256

Query: 295 ALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFR 354
           + SKR                                    + V++IE  A         
Sbjct: 257 SSSKR------------------------------------SRVKAIEAGAVWILIELLP 316

Query: 355 GSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWIS 414
            SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWIS
Sbjct: 317 ESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWIS 376

Query: 415 SFHPNERVVEEMMACGVVKKLMMILHMDG---GGGGRSSTKEKAIKILKMHGSSWRRSPC 474
           SFHP ERVV+EMMACG VKKL+MILHMD    G GGRSSTKEK IKILKMHG+ WRRSPC
Sbjct: 377 SFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPC 415

Query: 475 FPSELKNYLMFVNIE 484
           FPSELKNYL FVN E
Sbjct: 437 FPSELKNYLKFVNDE 415

[Close]

BLAST of CmaCh06G012110 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 3.5e-171
Identity = 331/433 (76.44%), Postives = 354/433 (81.76%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAG--DFVITPNLNLKRLILAW 114
           LMEDPVTISTGVTYERKSIEKWLFTYN +TCPATMQT+A G  DFV+TPNL LKR+ILAW
Sbjct: 29  LMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTPNLTLKRVILAW 88

Query: 115 KITDSSASPAT-IEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVV 174
           KI +SS+SPAT I++V+SLLATVESSPFKVSSLRKLRSMIE  DE+KS F+RLNGIGIVV
Sbjct: 89  KINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRDEVKSEFIRLNGIGIVV 148

Query: 175 NVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSA 234
           NVVVQILIDCSDF T EACEEA+GVLSQ PISNED+LFDEMSKREELMKS+AIVLQRGSA
Sbjct: 149 NVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKREELMKSVAIVLQRGSA 208

Query: 235 EGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEIL 294
           EGRFYAVKILRNIAKNTN+NWG V+EEQGID FKPLLELVSDEF TK+SSCALDVM+EIL
Sbjct: 209 EGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEIL 268

Query: 295 ALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFR 354
           A SKR                                    + V++IE  A         
Sbjct: 269 ASSKR------------------------------------SRVKAIEAGAVWILIELLP 328

Query: 355 GSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWIS 414
            S RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATK+GVKIL WIS
Sbjct: 329 ESSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWIS 388

Query: 415 SFHPNERVVEEMMACGVVKKLMMILHM---DGGGGGRSSTKEKAIKILKMHGSSWRRSPC 474
           SFHP ERVVEEMMACG VKKL+MILHM     GGGGRSSTKEK IKILKMHGSSWRRSPC
Sbjct: 389 SFHPKERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPC 425

Query: 475 FPSELKNYLMFVN 482
           FP ELKNYL  VN
Sbjct: 449 FPCELKNYLKLVN 425

[Close]

BLAST of CmaCh06G012110 vs. NCBI nr
Match: XP_022974967.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima] >XP_022975519.1 E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 708.0 bits (1826), Expect = 5.6e-200
Identity = 376/429 (87.65%), Postives = 379/429 (88.34%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           K                                     + V++IE  A          SG
Sbjct: 257 KS------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELK 474
           PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELK
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELK 409

Query: 475 NYLMFVNIE 484
           NYLMFVNIE
Sbjct: 437 NYLMFVNIE 409

[Close]

BLAST of CmaCh06G012110 vs. NCBI nr
Match: KAG7028702.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 699.1 bits (1803), Expect = 2.6e-197
Identity = 375/433 (86.61%), Postives = 378/433 (87.30%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL LKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLTLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKS FVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           KR                                    + V++IE  A          SG
Sbjct: 257 KR------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMD----GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 474
           PNERVVEEMMACGVVKKLMMILHMD    GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 413

Query: 475 SELKNYLMFVNIE 484
           SELKNYLMFVNIE
Sbjct: 437 SELKNYLMFVNIE 413

[Close]

BLAST of CmaCh06G012110 vs. NCBI nr
Match: XP_022959873.1 (E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 697.6 bits (1799), Expect = 7.6e-197
Identity = 374/433 (86.37%), Postives = 377/433 (87.07%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL LKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLTLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKS FVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC LDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCTLDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           KR                                    + V++IE  A          SG
Sbjct: 257 KR------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMD----GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 474
           PNERVVEEMMACGVVKKLMMILHMD    GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 413

Query: 475 SELKNYLMFVNIE 484
           SELKNYLMFVNIE
Sbjct: 437 SELKNYLMFVNIE 413

[Close]

BLAST of CmaCh06G012110 vs. NCBI nr
Match: KAG6597231.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697.2 bits (1798), Expect = 1.0e-196
Identity = 374/433 (86.37%), Postives = 377/433 (87.07%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNL LKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLTLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKS FVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           KR                                    + V++IE  A          SG
Sbjct: 257 KR------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRL LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMD----GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 474
           PNERVVEEMMACGVVKKLMMILHMD    GGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 413

Query: 475 SELKNYLMFVNIE 484
           SELKNYLMFVNIE
Sbjct: 437 SELKNYLMFVNIE 413

[Close]

BLAST of CmaCh06G012110 vs. NCBI nr
Match: XP_023539069.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 2.1e-191
Identity = 362/416 (87.02%), Postives = 366/416 (87.98%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI
Sbjct: 17  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 76

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFVRLNGIGIVVNVV 174
           TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKS FVRLNGIGIVVNVV
Sbjct: 77  TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSEFVRLNGIGIVVNVV 136

Query: 175 VQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREELMKSIAIVLQRGSAEGR 234
           VQILIDCSDFWTFEACEEAVGVLSQLPIS+EDKLFDEMSKREELMKSIAIVLQRGSAEGR
Sbjct: 137 VQILIDCSDFWTFEACEEAVGVLSQLPISSEDKLFDEMSKREELMKSIAIVLQRGSAEGR 196

Query: 235 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 294
           FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS
Sbjct: 197 FYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSCALDVMMEILALS 256

Query: 295 KRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEMRANAADSAEFRGSG 354
           KR                                    + V++IE  A          SG
Sbjct: 257 KR------------------------------------SRVKAIEAGAVCILIELLPESG 316

Query: 355 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 414
           RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH
Sbjct: 317 RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFH 376

Query: 415 PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 471
           PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP
Sbjct: 377 PNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMHGSSWRRSPCFP 396

[Close]

BLAST of CmaCh06G012110 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-42
Identity = 130/434 (29.95%), Postives = 211/434 (48.62%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R SIEKWLF     +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 24  IMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD--LTPNHTLRRLIQSWCT 83

Query: 115 TDSS---------ASPATIEEVRSLLA-TVESSPFKVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+  +  S   +V  L++LR ++  +   K      
Sbjct: 84  LNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSENATNKRCLEAA 143

Query: 175 NGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREE--LMKSI 234
                + N+V       +D       +EA+ +L  L  S E  L + ++ +++  ++KS+
Sbjct: 144 GVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVLKNLLNNKKDNNIVKSL 203

Query: 235 AIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKASSC 294
             ++QRG  E R YA  +L+NI +  +      L+    + F  +++++ D  S KA+  
Sbjct: 204 TKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEVVQILDDRISQKATKA 263

Query: 295 ALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRAL-TGVRS--IE 354
           A+ +++ I                 C  G               + H+A+  GV S  IE
Sbjct: 264 AMHILVNI-----------------CPWGR--------------NRHKAVEAGVISVIIE 323

Query: 355 MRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVA 414
           +  + + ++E RG      E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A
Sbjct: 324 LLMDESFTSERRGP-----EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTA 383

Query: 415 TKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKILKMH 474
           +   V++LL +  F     ++ EM+  GVV KL ++L +  GG     TKEKA ++LK+H
Sbjct: 384 SDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGG----KTKEKAKELLKLH 405

[Close]

BLAST of CmaCh06G012110 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 163.3 bits (412), Expect = 4.8e-40
Identity = 131/456 (28.73%), Postives = 210/456 (46.05%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           +M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +   D  +TPN  L+RLI +W  
Sbjct: 19  IMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LTPNHTLRRLIQSWCT 78

Query: 115 TDSS---------ASPATIEEVRSLLATVESSPF-KVSSLRKLRSMIETSDEMKSGFVRL 174
            ++S           P    E+  L+    SS   +V  L++LR ++  +   K      
Sbjct: 79  LNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVSENTTNKRCLEAA 138

Query: 175 NGIGIVVNVVVQIL-------IDCSDFWTFEAC----------------EEAVGVLSQLP 234
                + N+V   +          S     + C                +EA+ VL  L 
Sbjct: 139 EVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSLMDEALSVLYHLD 198

Query: 235 ISNED-KLFDEMSKREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQG 294
            S    K      K   L+K++  ++QRG  E R YA  +L+ + +  +     +LE   
Sbjct: 199 TSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVADPMQIILLER-- 258

Query: 295 IDFFKPLLELVSDEFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGD 354
            + F  +++++ D+ S KA+  A+ +++                 I C  G         
Sbjct: 259 -ELFGEVIQILHDQISHKATRSAMQILV-----------------ITCPWGR-------- 318

Query: 355 RGRCCVHPHRALTG---VRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLA 414
                 + H+A+ G      IE+  +   S+E R S     E  + +L  LC+CAEGR  
Sbjct: 319 ------NRHKAVEGGTISMIIELLMDDTFSSERRNS-----EMAMVVLDMLCQCAEGRAE 378

Query: 415 LVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILH 474
            + HG  IA V+KKIL VS + ++  V++LL +  F     +++EM+  GVV KL ++L 
Sbjct: 379 FLNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ 429

[Close]

BLAST of CmaCh06G012110 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 129.8 bits (325), Expect = 5.9e-30
Identity = 120/445 (26.97%), Postives = 207/445 (46.52%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAW-- 114
           +M+DPVT  +G+TY+R++I KWL      +CP T Q L   D  +TPN  L+RLI  W  
Sbjct: 36  IMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL-DSDLTPNHMLRRLIQHWCV 95

Query: 115 --------KITDSSASPA---TIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGF 174
                   +I+     P     +EE+++L    + +  +  +L+KL  +    +  +   
Sbjct: 96  ENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEVLAMDGNNRRLMC 155

Query: 175 VRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPI-SNEDKLFDEMSKREELMK 234
                  +++ VV     D       +  +E++ +L  + I SN+ K    + + + +M+
Sbjct: 156 ECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKTI--LMENDRVME 215

Query: 235 SIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEFSTKAS 294
           S+  VL +     + Y + +LRN+   T +    ++E    + FK ++  + D  ++  +
Sbjct: 216 SLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPEIFKGIIGFLKDVVNS-VN 275

Query: 295 SCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALTGVRSIEM 354
             +  V   + + S+  L K +  ++   D +  I          +    + +  RS+ +
Sbjct: 276 RTSPTVRETVQSSSRPSLGKTEPSKL---DHSLVIKQAVTAALMILLETSSWSRNRSLLV 335

Query: 355 RANAAD---SAEFRGSGRSK-CERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 414
              A       E   +G  +  E ML +L  LC CA GR  ++ H  GIA VTK++L VS
Sbjct: 336 DLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLLRVS 395

Query: 415 NVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKIL 474
             A    + IL  +S F P   VVEEM+  G V+KL  +L MD G     + KEKA +IL
Sbjct: 396 PAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG----LNLKEKAKEIL 455

Query: 475 KMHGSSWRRSPCFPSELKNYLMFVN 482
           K H   W++ PC    L   L+ ++
Sbjct: 456 KDHFDEWKKFPCIDITLLTKLLSIS 464

[Close]

BLAST of CmaCh06G012110 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 112.5 bits (280), Expect = 9.8e-25
Identity = 118/428 (27.57%), Postives = 188/428 (43.93%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPVT+ TG TY+R SIE W+   NN TCP T   L+  DF + PN  L+RLI  W +
Sbjct: 26  LMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLIPNHTLRRLIQEWCV 85

Query: 115 TDSS---------ASPATIEEVRSLLA-------TVESSPFKVSSLRKLRSMIETSDEMK 174
            + S           PA    VR+LL+       T  S   + ++LR+LR     SD+ +
Sbjct: 86  ANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRGFARDSDKNR 145

Query: 175 SGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKREEL 234
                 N   I++ ++       S+  + E   E++ +L  LPI+ E   F  +S     
Sbjct: 146 VLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-EPNQFVSISSDPGR 205

Query: 235 MKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFN--WGSVLEEQGIDFFKPLLELVSDEFS 294
           ++ +  +L   S E R  A  ++  ++  T      GS+   + +  F+ +L+L+ +  S
Sbjct: 206 VEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV--FEGVLDLLRNPIS 265

Query: 295 TKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT-GV 354
           ++                       + ++IG        S +  R       H A+T G 
Sbjct: 266 SR-----------------------RALKIGIKTLFALCSVKSTR-------HIAITAGA 325

Query: 355 RSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNV 414
             I +   AAD        R   ER L  ++ LC   EG  A  EH + +  + K IL V
Sbjct: 326 PEILIDRLAAD------FDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRV 385

Query: 415 SNVATKMGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEKAIKI 464
           S+ AT+     LL + +    ER  EE    GVV +L++++  +         K+KA K+
Sbjct: 386 SDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE----CTERAKKKAQKL 400

[Close]

BLAST of CmaCh06G012110 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.3e-21
Identity = 117/441 (26.53%), Postives = 193/441 (43.76%), Query Frame = 0


Query: 55  LMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLAAGDFVITPNLNLKRLILAWKI 114
           LM+DPV ISTG+TY+R SIE W+    NKTCP T   L   D +  PN  ++++I  W +
Sbjct: 43  LMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTTFDQI--PNHTIRKMIQGWCV 102

Query: 115 TDSSASPATIEEVRSLLATVESSPFKVSSLRKLRSMIETSDEMKSGFV--RLNGIG---- 174
              S     I   R  L   E   +++S  RKL S     D  K G +  ++  +G    
Sbjct: 103 EKGSPLIQRIPTPRVPLMPCE--VYEIS--RKLSSATRRGDYEKCGVIIEKIKKLGDESE 162

Query: 175 -----IVVNVVVQILIDCSDFWTFEACEEAVGVLSQL--------PISNE--DKLFDEMS 234
                +  N V  +L DC D   F   E+   +L+++        PI  E   KL    S
Sbjct: 163 KNRKCVNENSVGWVLCDCFD--KFSGDEKLTFMLNEILSLLTWMFPIGLEGISKLASATS 222

Query: 235 KREELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSD 294
                 + +A +L+      R  A  I++ I         S   E G+   + L++L+ D
Sbjct: 223 -----FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV--AEALVKLIRD 282

Query: 295 EFSTKASSCALDVMMEILALSKRILHKGKLVRIGCNDGNFSISSEGDRGRCCVHPHRALT 354
             S+ ++  +L      +A+ + +L K ++       G  SI                  
Sbjct: 283 SVSSSSTKSSL------IAIYQMVLQKPEIASEFLEIGLVSI------------------ 342

Query: 355 GVRSIEMRANAADSAEFRGSGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKIL 414
              ++EM  +A +S          CE+ L +L ++CE   GR  + ++ + +  + KKI 
Sbjct: 343 ---TVEMIVDAENSV---------CEKALAVLDAICETEHGREEVRKNALVMPLLVKKIA 402

Query: 415 NVSNVATK--MGVKILLWISSFHPNERVVEEMMACGVVKKLMMILHMDGGGGGRSSTKEK 472
            VS +AT+  M + + LW +    N   VE+ +  G  +K++++L +  G      TKEK
Sbjct: 403 KVSELATRSSMSMILKLWKTG---NTVAVEDAVRLGAFQKVLLVLQVGYG----EETKEK 424

The following BLAST results are available for this feature:

BLAST of CmaCh06G012110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	1.9e-41	29.95	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	6.8e-39	28.73	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	8.3e-29	26.97	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	1.4e-23	27.57	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q5PNY6	1.9e-20	26.53	U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2...	[more]

BLAST of CmaCh06G012110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IKT9	2.7e-200	87.65	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 P...	[more]
A0A6J1H765	3.7e-197	86.37	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728...	[more]
A0A6J1H5I9	2.2e-189	85.95	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728...	[more]
A0A0A0L6J2	1.8e-172	75.86	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE...	[more]
A0A6J1IYW3	3.5e-171	76.44	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 P...	[more]

BLAST of CmaCh06G012110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022974967.1	5.6e-200	87.65	E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima] >XP_022975519.1 E3 ubi...	[more]
KAG7028702.1	2.6e-197	86.61	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022959873.1	7.6e-197	86.37	E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata]	[more]
KAG6597231.1	1.0e-196	86.37	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023539069.1	2.1e-191	87.02	E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh06G012110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	1.4e-42	29.95	plant U-box 23	[more]
AT3G52450.1	4.8e-40	28.73	plant U-box 22	[more]
AT3G11840.1	5.9e-30	26.97	plant U-box 24	[more]
AT3G19380.1	9.8e-25	27.57	plant U-box 25	[more]
AT5G37490.1	1.3e-21	26.53	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 52..112 e-value: 8.2E-12 score: 55.2
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 55..113 e-value: 2.4E-9 score: 37.3
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 55..119 score: 11.474638
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 117..464 e-value: 9.6E-9 score: 36.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 52..115 e-value: 2.1E-15 score: 58.4
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 55..300
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 354..481
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 55..300
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 354..481
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 54..115
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 123..437

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G012110.1	CmaCh06G012110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity