CmaCh06G011210 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G011210
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Cytochrome P450
Location	Cma_Chr06: 7546709 .. 7547609 (+)
RNA-Seq Expression	CmaCh06G011210
Synteny	CmaCh06G011210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAACGATAACATATTGTCGAGATTTCTGCAAGCGGAAAAGACCGATCCAACGTATCTAAGAGATATTATTCTAAACTTCGTCATAGCTGGAAGGGACACAATAGCAGTTACCCTTGCTTGGTTCATTTACATGCTTTGCAAGCATCCTGCAGAGGAGAAAATTTTCCTGGTAAGCTAATTGGTCCATGTTCGACTCTGTGAACGGTTGGATATGGTCCATGATCAAAAACGTTAAGCACCTATGCTTTTCAATTCTGTTTGTTGTTATGAATAGAGCATAACCTTACGAGTATTTATAAGGGAGAAAACACTAACAAATTTTCTAAACTTTCTCCTTGCGTTAGCAACTACTAACAATATAACTACGGCCGATTATCCTCTAAGGTAATAATTTCTTTTGCTAAGTCAAGCTTTTTAACCGTTCATGTCTAGTCAGCCTTGTAGCTTCCAAGTTTTAGGCAAGTTTCATATTTTTCGGCGGAATGAATCGACTCAAAATGTTAAGTTTTCGAATTGCAGGATGCGAAGATTTGGTTTTCTGATGATATTCTACCAGACGGATTTAGTGCGAGAAAAGGAGATATGGTGTCATACCAACCATATGCAATGGGTAGGATGAAGTTCATATGGGGTGACGACGCCGAGGATTTCCGACCCGAGCGATGGTTCGACCGAGATGGAAAGCTTCAGCCTCAGAGCCCTTTCAAATTCACAGCCTTTCAGGTATGACTAATACAATTCAAACAAAATGGTTTGCTCTGGAGAAAGTGCATAATAGTATTGTTTATAAGACTGATGGTTCACAGGCAGGGCCACGCATATGTCTGGGGAAAGAGTTTGCTTATAGGCAGATAAAAATCCCCTATTTTCGGGCTGCTAAACTTGGGTGGGTCTAA

mRNA sequence

ATGAGGAACGATAACATATTGTCGAGATTTCTGCAAGCGGAAAAGACCGATCCAACGTATCTAAGAGATATTATTCTAAACTTCGTCATAGCTGGAAGGGACACAATAGCAGTTACCCTTGCTTGGTTCATTTACATGCTTTGCAAGCATCCTGCAGAGGAGAAAATTTTCCTGGATGCGAAGATTTGGTTTTCTGATGATATTCTACCAGACGGATTTAGTGCGAGAAAAGGAGATATGGTGTCATACCAACCATATGCAATGGGTAGGATGAAGTTCATATGGGGTGACGACGCCGAGGATTTCCGACCCGAGCGATGGTTCGACCGAGATGGAAAGCTTCAGCCTCAGAGCCCTTTCAAATTCACAGCCTTTCAGGCAGGGCCACGCATATGTCTGGGGAAAGAGTTTGCTTATAGGCAGATAAAAATCCCCTATTTTCGGGCTGCTAAACTTGGGTGGGTCTAA

Coding sequence (CDS)

ATGAGGAACGATAACATATTGTCGAGATTTCTGCAAGCGGAAAAGACCGATCCAACGTATCTAAGAGATATTATTCTAAACTTCGTCATAGCTGGAAGGGACACAATAGCAGTTACCCTTGCTTGGTTCATTTACATGCTTTGCAAGCATCCTGCAGAGGAGAAAATTTTCCTGGATGCGAAGATTTGGTTTTCTGATGATATTCTACCAGACGGATTTAGTGCGAGAAAAGGAGATATGGTGTCATACCAACCATATGCAATGGGTAGGATGAAGTTCATATGGGGTGACGACGCCGAGGATTTCCGACCCGAGCGATGGTTCGACCGAGATGGAAAGCTTCAGCCTCAGAGCCCTTTCAAATTCACAGCCTTTCAGGCAGGGCCACGCATATGTCTGGGGAAAGAGTTTGCTTATAGGCAGATAAAAATCCCCTATTTTCGGGCTGCTAAACTTGGGTGGGTCTAA

Protein sequence

MRNDNILSRFLQAEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHPAEEKIFLDAKIWFSDDILPDGFSARKGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAGPRICLGKEFAYRQIKIPYFRAAKLGWV

Homology

[Close]

BLAST of CmaCh06G011210 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.6e-37
Identity = 84/201 (41.79%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 5   NILSRFLQAE------KTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKIF 64
           +ILSR++         K    YLRD+ILNF++A RDT A+ L+WFIYMLCKH   +EK+ 
Sbjct: 274 DILSRYIIISDKETDGKVSDKYLRDVILNFMVAARDTTAIALSWFIYMLCKHQHVQEKLL 333

Query: 65  ------------------------------------------------------LDAKIW 124
                                                                 +D K  
Sbjct: 334 EEIISSTSVHEDQYSTECNDIASFAQSLTDEALGKMHYLHASLSETLRLYPALPVDGKYV 393

Query: 125 FSDDILPDGFSARKGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFT 145
            ++D LPDGF  +KGD V++ PYAMGRM ++WGDDA++F+PERW  +DG   P+SPFKF 
Sbjct: 394 VNEDTLPDGFKVKKGDSVNFLPYAMGRMSYLWGDDAKEFKPERWI-QDGIFHPKSPFKFP 453

[Close]

BLAST of CmaCh06G011210 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 2.4e-30
Identity = 68/185 (36.76%), Postives = 105/185 (56.76%), Query Frame = 0


Query: 1   MRNDNILSRFLQAEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKIF-- 60
           +  +++LSRFL +  +D  ++ DI+++F++AG+DT +  L WF ++L K+P  EE+I   
Sbjct: 285 LETEDMLSRFLSSGHSDEDFVADIVISFILAGKDTTSAALTWFFWLLWKNPRVEEEIVNE 344

Query: 61  ----------------------------------LDAKIWFSDDILPDGFSARKGDMVSY 120
                                             +D+K   +DD+LPDG+  +KG +V+Y
Sbjct: 345 LSKKSELMVYDEVKEMVYTHAALSESMRLYPPVPMDSKEAVNDDVLPDGWVVKKGTIVTY 404

Query: 121 QPYAMGRMKFIWGDDAEDFRPERWFDRD---GK--LQPQSPFKFTAFQAGPRICLGKEFA 144
             YAMGRMK +WGDD  +FRPERW ++D   GK     +  + +  FQAGPR+CLGKE A
Sbjct: 405 HVYAMGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVFQAGPRVCLGKEMA 464

[Close]

BLAST of CmaCh06G011210 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-29
Identity = 66/187 (35.29%), Postives = 97/187 (51.87%), Query Frame = 0


Query: 1   MRNDNILSRFLQ-AEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHPAEEK---- 60
           M  ++++SRF+    + D  YLRDI+++F++AGRDT+A  L  F ++L +HP  E     
Sbjct: 264 MGKNDLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIRE 323

Query: 61  ---------------------------------------IFLDAKIWFSDDILPDGFSAR 120
                                                  +  D+K   +DD+L DG    
Sbjct: 324 ELDRVMGTGFDSVTARCDEMREMDYLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVN 383

Query: 121 KGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAGPRICLGKE 144
            G  V+Y  YAMGRM  IWG D E+F+PERW D +GK +P++P K+  FQAG R+C+GKE
Sbjct: 384 SGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGKFRPENPVKYPVFQAGARVCIGKE 443

[Close]

BLAST of CmaCh06G011210 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.5e-29
Identity = 72/194 (37.11%), Postives = 101/194 (52.06%), Query Frame = 0


Query: 2   RNDNILSRFL-QAEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKIFL- 61
           ++D++LSRF+ + E     +L+ + LNF++AGRDT +V L+WF +++  +P  EEKI + 
Sbjct: 277 QHDDLLSRFMKKKESYSDKFLQHVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVE 336

Query: 62  ------------------------------------------------DAKIWFSDDILP 121
                                                           D+K   SDD LP
Sbjct: 337 ICTILAETRGNDTSKWLEEPLVFEEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLP 396

Query: 122 DGFSARKGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDG-KLQPQSPFKFTAFQAGP 144
           DG     G  ++Y  Y+ GRMKFIWG+D  +F+PERW  +DG K Q Q  F+F AF AGP
Sbjct: 397 DGTFVPAGSNITYSIYSTGRMKFIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGP 456

[Close]

BLAST of CmaCh06G011210 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.6e-29
Identity = 72/184 (39.13%), Postives = 93/184 (50.54%), Query Frame = 0


Query: 12  QAEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKIF------------- 71
           + E     +LRDI +NF++AGRDT +V L+WF ++L K+P  EEKI              
Sbjct: 317 KGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILRQRDDH 376

Query: 72  --------------------------------------LDAKIWFSDDILPDGFSARKGD 131
                                                 +D K    DD+ PDG   +KGD
Sbjct: 377 GNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTMLKKGD 436

Query: 132 MVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAGPRICLGKEFAY 144
            V Y  YAMGRM+ IWG D  +FRPERW  RDG+   +S +KFTAF  GPR+CLGK+FAY
Sbjct: 437 KVIYAIYAMGRMEAIWGKDCLEFRPERWL-RDGRFMSESAYKFTAFNGGPRLCLGKDFAY 496

[Close]

BLAST of CmaCh06G011210 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-40
Identity = 89/196 (45.41%), Postives = 111/196 (56.63%), Query Frame = 0


Query: 4   DNILSRFLQAEKTDP-----TYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKIF 63
           ++ILSRFL   + DP      YLRDIILNF+IAG+DT A  L+WF+YMLCK+P  +EKI 
Sbjct: 273 EDILSRFLVESEKDPENMNDKYLRDIILNFMIAGKDTTAALLSWFLYMLCKNPLVQEKIV 332

Query: 64  -------------------------------------------------LDAKIWFSDDI 123
                                                            +D +   +DD+
Sbjct: 333 QEIRDVTFSHEKTTDVNGFVESINEEALDEMHYLHAALSETLRLYPPVPVDMRCAENDDV 392

Query: 124 LPDGFSARKGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAG 145
           LPDG    KGD + Y  YAMGRM +IWG DAE+F+PERW  +DG  QP+SPFKF +F AG
Sbjct: 393 LPDGHRVSKGDNIYYIAYAMGRMTYIWGQDAEEFKPERWL-KDGLFQPESPFKFISFHAG 452

[Close]

BLAST of CmaCh06G011210 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 154.1 bits (388), Expect = 9.4e-38
Identity = 84/196 (42.86%), Postives = 112/196 (57.14%), Query Frame = 0


Query: 4   DNILSRFLQAEKTDP-----TYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKI- 63
           ++ILS+FL   + DP      YLRDIILN ++AG+DT A +L+WF+YMLCK+P  +EKI 
Sbjct: 267 EDILSKFLLESEKDPENMNDKYLRDIILNVMVAGKDTTAASLSWFLYMLCKNPLVQEKIV 326

Query: 64  ------------------FLDA------------------------------KIWFSDDI 123
                             F+++                              +   +DD+
Sbjct: 327 QEIRDVTSSHEKTTDVNGFIESVTEEALAQMQYLHAALSETMRLYPPVPEHMRCAENDDV 386

Query: 124 LPDGFSARKGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAG 145
           LPDG    KGD + Y  YAMGRM +IWG DAE+F+PERW  +DG  QP+S FKF +F AG
Sbjct: 387 LPDGHRVSKGDNIYYISYAMGRMTYIWGQDAEEFKPERWL-KDGVFQPESQFKFISFHAG 446

[Close]

BLAST of CmaCh06G011210 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 130.6 bits (327), Expect = 1.1e-30
Identity = 66/187 (35.29%), Postives = 97/187 (51.87%), Query Frame = 0


Query: 1   MRNDNILSRFLQ-AEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHPAEEK---- 60
           M  ++++SRF+    + D  YLRDI+++F++AGRDT+A  L  F ++L +HP  E     
Sbjct: 264 MGKNDLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIRE 323

Query: 61  ---------------------------------------IFLDAKIWFSDDILPDGFSAR 120
                                                  +  D+K   +DD+L DG    
Sbjct: 324 ELDRVMGTGFDSVTARCDEMREMDYLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVN 383

Query: 121 KGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAGPRICLGKE 144
            G  V+Y  YAMGRM  IWG D E+F+PERW D +GK +P++P K+  FQAG R+C+GKE
Sbjct: 384 SGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGKFRPENPVKYPVFQAGARVCIGKE 443

[Close]

BLAST of CmaCh06G011210 vs. TAIR 10
Match: AT2G44890.2 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 130.6 bits (327), Expect = 1.1e-30
Identity = 78/198 (39.39%), Postives = 104/198 (52.53%), Query Frame = 0


Query: 2   RNDNILSRFLQAEKTDP-----TYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEK 61
           +N +ILS+FL   + DP      YLRDIILN ++AG+DT A +L+WF+YMLCK+P  +EK
Sbjct: 267 QNTDILSKFLLESEKDPENMNDKYLRDIILNVMVAGKDTTAASLSWFLYMLCKNPLVQEK 326

Query: 62  I-------------------FLDA------------------------------KIWFSD 121
           I                   F+++                              +   +D
Sbjct: 327 IVQEIRDVTSSHEKTTDVNGFIESVTEEALAQMQYLHAALSETMRLYPPVPEHMRCAEND 386

Query: 122 DILPDGFSARKGDMVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQ 145
           D+LPDG    KGD + Y  YAMGRM +IWG DAE+F+PERW  +DG              
Sbjct: 387 DVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAEEFKPERWL-KDG-------------- 446

[Close]

BLAST of CmaCh06G011210 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 129.0 bits (323), Expect = 3.2e-30
Identity = 72/184 (39.13%), Postives = 93/184 (50.54%), Query Frame = 0


Query: 12  QAEKTDPTYLRDIILNFVIAGRDTIAVTLAWFIYMLCKHP-AEEKIF------------- 71
           + E     +LRDI +NF++AGRDT +V L+WF ++L K+P  EEKI              
Sbjct: 317 KGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILRQRDDH 376

Query: 72  --------------------------------------LDAKIWFSDDILPDGFSARKGD 131
                                                 +D K    DD+ PDG   +KGD
Sbjct: 377 GNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTMLKKGD 436

Query: 132 MVSYQPYAMGRMKFIWGDDAEDFRPERWFDRDGKLQPQSPFKFTAFQAGPRICLGKEFAY 144
            V Y  YAMGRM+ IWG D  +FRPERW  RDG+   +S +KFTAF  GPR+CLGK+FAY
Sbjct: 437 KVIYAIYAMGRMEAIWGKDCLEFRPERWL-RDGRFMSESAYKFTAFNGGPRLCLGKDFAY 496

The following BLAST results are available for this feature:

BLAST of CmaCh06G011210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK3	1.6e-37	41.79	Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1	[more]
O81117	2.4e-30	36.76	Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2	[more]
Q9ZUX1	1.6e-29	35.29	Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1	[more]
B3RFJ6	3.5e-29	37.11	Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1	[more]
Q9FMY1	4.6e-29	39.13	Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1	[more]

BLAST of CmaCh06G011210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45510.1	1.5e-40	45.41	cytochrome P450, family 704, subfamily A, polypeptide 2	[more]
AT2G44890.1	9.4e-38	42.86	cytochrome P450, family 704, subfamily A, polypeptide 1	[more]
AT2G27690.1	1.1e-30	35.29	cytochrome P450, family 94, subfamily C, polypeptide 1	[more]
AT2G44890.2	1.1e-30	39.39	cytochrome P450, family 704, subfamily A, polypeptide 1	[more]
AT5G23190.1	3.2e-30	39.13	cytochrome P450, family 86, subfamily B, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 31..48 score: 35.98 coord: 123..132 score: 48.47 coord: 132..143 score: 34.22
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 67..144 e-value: 2.3E-16 score: 59.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 61..148 e-value: 1.4E-23 score: 85.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..59 e-value: 5.7E-11 score: 44.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..144
None	No IPR available	PANTHER	PTHR24296:SF210	CYTOCHROME P450-RELATED	coord: 57..144
None	No IPR available	PANTHER	PTHR24296:SF210	CYTOCHROME P450-RELATED	coord: 3..52
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 57..144
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 3..52

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G011210.1	CmaCh06G011210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051641	cellular localization
biological_process	GO:0006887	exocytosis
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen