CmaCh06G006940 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G006940 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 6.3e-225
Identity = 402/764 (52.62%), Postives = 525/764 (68.72%), Query Frame = 0

Query: 11  LLFFFLLQTSSVH-----TKKSYIVYLGSESNPSIYD---VQLATESKYDILGTVKGSKV 70
           LL   L+  SS H        SY+VY G+ S+        +    E+ YD LG+  GS+ 
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 71  AAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSD 130
            A ++I Y+Y + INGFAA LD      ++K+P VVS+F N+  KLHTTRSW FLG+  +
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 131 RGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEG--GSNFSC 190
             +P +SIW+ ++FGED II NLDT    ES SF D G GP+PSRW G C+    + F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189

Query: 191 NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 250
           NRKLIGARYF +GY    G LN S  + RD +GHG+HTLSTA G+FV G ++FG GNGTA
Sbjct: 190 NRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTA 249

Query: 251 KGGAPKARVAAYKVCWPQGK---CSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPI 310
           KGG+P+ARVAAYKVCWP  K   C DAD+LA  +AAI DG DV+S+SLG     F +D +
Sbjct: 250 KGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSV 309

Query: 311 SVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVK 370
           ++G+FHA ++ I+VVCSAGN GP   TV+NV+PW  TVGAS++DR F S + LGN K  K
Sbjct: 310 AIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYK 369

Query: 371 GSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGR 430
           G SLSS  LP  K YP+M SVNAKA NAS   AQLC+ GSLDP+K +GKI+VCLRG NGR
Sbjct: 370 GQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGR 429

Query: 431 MDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVAS 490
           ++K   V   GG+GM+L N  ++G+D+  D H+LP + ++  D  +++ Y+  TK+P+A 
Sbjct: 430 VEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAH 489

Query: 491 ITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDK 550
           ITP RT++G+KP+PVMA FSS+GP+ +A  ++KPDI+APGV++IA++T AV+ T+  FD 
Sbjct: 490 ITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDP 549

Query: 551 RRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNK 610
           RR+ FN  SGTSMSCPHI+G+AGLLK  +P+WSPAAI+SAIMTTA   D+    + +   
Sbjct: 550 RRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN 609

Query: 611 VKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK-N 670
           +KATPF +GAGHV PN A++PGLVYD  I DYLNFLC  GYN+  +  FS   F C+   
Sbjct: 610 MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPK 669

Query: 671 FASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHS 730
            +  +LNYPSI+VP L     VTV+R VKNVG    Y  +V  P+G+ V V+P++L F  
Sbjct: 670 ISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTK 729

Query: 731 VGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 757
           VGE+K FK++   ++     GYVFG LVWSD KH VRSPI V L
Sbjct: 730 VGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmaCh06G006940 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 2.4e-224
Identity = 410/762 (53.81%), Postives = 512/762 (67.19%), Query Frame = 0

Query: 7   ISPLLLFFFLLQTSSVHTKKSYIVYLGSESN-PSIYDVQL--ATESKYDILGTVKGSKVA 66
           +S LLL   L  + +   KKSYIVYLGS ++ P I    L     S    L +  GS   
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN 80

Query: 67  AKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDR 126
           AKE+I Y+Y R INGFAA+LD+ E   +AK+P VVS+F N+ RKLHTT SW+F+ +  + 
Sbjct: 81  AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 127 GIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSCNRK 186
            +  +S+W  + +GED II NLDT    ES SF D GYG VP+RW G C    +  CNRK
Sbjct: 141 VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRK 200

Query: 187 LIGARYFYRGYEMING-PLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKG 246
           LIGARYF +GY    G P N S    RDH+GHG+HTLSTA GNFV GANVFG GNGTA G
Sbjct: 201 LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 247 GAPKARVAAYKVCWPQ---GKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISV 306
           G+PKARVAAYKVCWP     +C DAD+LA IEAAI DGVDVLS S+G  A D+  D I++
Sbjct: 261 GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 320

Query: 307 GAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGS 366
           G+FHA++ G+ VVCSAGN GP  GTV+NV+PW+ TVGASS+DR F ++V L N +  KG+
Sbjct: 321 GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 380

Query: 367 SLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMD 426
           SLS   LP  K+Y L+++ +A  +N +   A LC++GSLDP K +GKI+VCLRGDN R+D
Sbjct: 381 SLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVD 440

Query: 427 KSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASIT 486
           K  +    G  GM+L NDK SG++I +DAH+LP S + Y DG ++  YL STK P   I 
Sbjct: 441 KGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIK 500

Query: 487 PVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRR 546
                +  KP+P MA FSSRGPN I   ++KPDI+APGVNIIA+FT+A   TDL  D RR
Sbjct: 501 APTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRR 560

Query: 547 VPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVK 606
            PFN +SGTSMSCPHI+GV GLLK LHP WSPAAI+SAIMTT++TR+N +  M+D +  K
Sbjct: 561 TPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKK 620

Query: 607 ATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKP-FVCAKNFA 666
           A PF YG+GHV PN A  PGLVYD T  DYL+FLC  GYN+  ++ F+  P + C +   
Sbjct: 621 ANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGAN 680

Query: 667 STDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVG 726
             D NYPSI+VP L     +TV R++KNVG   TY AR R P G+ V VEP  L F+  G
Sbjct: 681 LLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 740

Query: 727 EEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 757
           E K F++       V   GYVFG L W+D  H+VRSPI V L
Sbjct: 741 EVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmaCh06G006940 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 689.5 bits (1778), Expect = 4.3e-197
Identity = 383/783 (48.91%), Postives = 510/783 (65.13%), Query Frame = 0

Query: 6   IISPLLLFFFLLQTSSVHTKKSYIVYLGSES---NPSIYDVQLATESKYDILGTVKGSKV 65
           I+S   LF FLL   +  +KK YIVY+G+ S   +P+  D++LAT+S YD+LG++ GS+ 
Sbjct: 11  ILSSFFLFTFLLAAVN-GSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSRE 70

Query: 66  AAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSD 125
            AKE+I+Y+YNR INGFAA+L+++E   +AKNP+VVS+F ++E KLHTTRSW FLG+   
Sbjct: 71  KAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGL-HR 130

Query: 126 RGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGA-CE-----GGS 185
           RG   NS W+  +FGE+ IIGN+DT    ES SF D GYG VPS+W G  C+     G  
Sbjct: 131 RG--QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSM 190

Query: 186 NFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNG 245
             +CNRKLIGARY+ + +E  NG L+     ARD  GHGTHTLSTAGGNFV GA VF  G
Sbjct: 191 KNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVG 250

Query: 246 NGTAKGGAPKARVAAYKVCW---PQGKCSDADLLAGIEAAISDGVDVLSISLGAA----A 305
           NGTAKGG+P+ARVAAYKVCW       C  AD+LA I+ AI DGVDV+++S G +    A
Sbjct: 251 NGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTA 310

Query: 306 QDFADDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVY 365
           +    D IS+GAFHAI + I++V SAGNDGP PGTV NV+PW+FT+ AS++DR F S + 
Sbjct: 311 EGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLT 370

Query: 366 LGNKKQVKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIV 425
           + N + ++G+SL    LP  + + L+ S +AK +NA+   AQLC  G+LD  K  GKI++
Sbjct: 371 I-NNQLIEGASLFV-NLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVL 430

Query: 426 CLR-GDNGRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYL 485
           C R G    + +  E L  G  GMIL N   +G  +  + H+  T +       S    +
Sbjct: 431 CTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGV 490

Query: 486 KST----------KRPVASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGV 545
           K+T                ++  RT  G KP+PVMA FSSRGPN I  +++KPD++APGV
Sbjct: 491 KTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGV 550

Query: 546 NIIASFTKAVAATDLPFDKRR-VPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSA 605
           NI+A++++  +A+ L  D RR   FNV  GTSMSCPH +G+AGLLK  HP+WSPAAIKSA
Sbjct: 551 NILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSA 610

Query: 606 IMTTAKTRDNTKNTMLD-FNKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQ 665
           IMTTA T DNT   + D F+K  A  F YG+GHV P+ A++PGLVYD ++ DYLNFLC  
Sbjct: 611 IMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCAS 670

Query: 666 GYNSLTLKKFS-NKPFVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVA 725
           GY+   +   + N+ F+C+ + +  DLNYPSI++P L++  PVT+ R V NVG   TY  
Sbjct: 671 GYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTV 730

Query: 726 RVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSP 755
             R P G ++ V P +L F  +GE K FK++   +    R  Y FG L W+DGKH VRSP
Sbjct: 731 STRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSP 786

BLAST of CmaCh06G006940 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 2.0e-170
Identity = 336/759 (44.27%), Postives = 463/759 (61.00%), Query Frame = 0

Query: 10  LLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGSKVAAKESIL 69
           LL   F   +SS   + +YIV++     PS +D+     + YD         ++    +L
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLH---SNWYD----SSLRSISDSAELL 73

Query: 70  YTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNS 129
           YTY   I+GF+  L  +E  +L   P V+S+      +LHTTR+  FLG+          
Sbjct: 74  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----D 133

Query: 130 IWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFS---CNRKLIG 189
           ++  +    DV++G LDT    ES S+ D G+GP+PS W G CE G+NF+   CNRKLIG
Sbjct: 134 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 193

Query: 190 ARYFYRGYEMINGPLNIS--SLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGA 249
           AR+F RGYE   GP++ S  S + RD +GHGTHT STA G+ V GA++ G  +GTA+G A
Sbjct: 194 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 253

Query: 250 PKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHA 309
           P+ARVA YKVCW  G C  +D+LA I+ AI+D V+VLS+SLG    D+  D +++GAF A
Sbjct: 254 PRARVAVYKVCW-LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 313

Query: 310 IQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSG 369
           +++GI+V CSAGN GP   +++NV+PW+ TVGA ++DR F +   LGN K   G SL  G
Sbjct: 314 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 373

Query: 370 GLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEV 429
                KL P + + N  ASNA++G   LC  G+L P K +GKI++C RG N R+ K   V
Sbjct: 374 EALPDKLLPFIYAGN--ASNATNG--NLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 433

Query: 430 LRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTE 489
              GGVGMIL N   +G ++  DAH+LP + V    G  I  Y+ +   P ASI+ + T 
Sbjct: 434 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTV 493

Query: 490 IGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNV 549
           +G+KPSPV+A FSSRGPN I   ++KPD+ APGVNI+A++T A   T L  D RRV FN+
Sbjct: 494 VGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNI 553

Query: 550 QSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVK-ATPF 609
            SGTSMSCPH++G+A LLK +HP WSPAAI+SA+MTTA         +LD    K +TPF
Sbjct: 554 ISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPF 613

Query: 610 DYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVC--AKNFASTD 669
           D+GAGHV P  A +PGL+YD T +DYL FLC   Y S  ++  S + + C  +K+++  D
Sbjct: 614 DHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVAD 673

Query: 670 LNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRM-PKGITVMVEPSTLQFHSVGEE 729
           LNYPS +V    +G      R V +VG AGTY  +V     G+ + VEP+ L F    E+
Sbjct: 674 LNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 733

Query: 730 KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVN 756
           K++ + F            FG++ WSDGKH V SP+A++
Sbjct: 734 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh06G006940 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 4.8e-164
Identity = 333/778 (42.80%), Postives = 471/778 (60.54%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS 60
           M  S I+  L L F  +  ++    K++I  +   S PSI+       S          +
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYS----------T 60

Query: 61  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 120
           + A +  I++ Y+   +GF+AV+   E   L  +P+V+++FE+R R+LHTTRS  FLG+ 
Sbjct: 61  EFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ 120

Query: 121 SDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFS- 180
           + +G     +W  S +G DVIIG  DT    E  SF D   GP+P RW G CE G+ FS 
Sbjct: 121 NQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSP 180

Query: 181 --CNRKLIGARYFYRGYE-MINGPLN--ISSLNARDHEGHGTHTLSTAGGNFVRGANVFG 240
             CNRK+IGAR+F +G +  + G +N  +  L+ RD +GHGTHT STA G     A++ G
Sbjct: 181 RNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSG 240

Query: 241 NGNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAA---AQD 300
             +G AKG APKAR+AAYKVCW    C D+D+LA  +AA+ DGVDV+SIS+G        
Sbjct: 241 YASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSP 300

Query: 301 FADDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLG 360
           +  DPI++G++ A  +GI V  SAGN+GP   +VTN++PW+ TVGAS+IDR F +   LG
Sbjct: 301 YYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILG 360

Query: 361 NKKQVKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCL 420
           +  +++G SL +G    G+++P++    +  S+AS     LC E +LDP + RGKI++C 
Sbjct: 361 DGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICD 420

Query: 421 RGDNGRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKST 480
           RG + R+ K   V + GGVGMIL N   +G  +  DAH++P   V   +G  I  Y  S 
Sbjct: 421 RGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSH 480

Query: 481 KRPVASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAAT 540
             P+ASI    T +GIKP+PV+A FS RGPN ++  ++KPD+ APGVNI+A++T AV  T
Sbjct: 481 PNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPT 540

Query: 541 DLPFDKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNT 600
            LP D R+  FN+ SGTSM+CPH++G A LLK  HP WSPA I+SA+MTT    DN+  +
Sbjct: 541 GLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRS 600

Query: 601 MLDFNKVK-ATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKP 660
           ++D +  K ATP+DYG+GH++   AM+PGLVYD T DDY+ FLC  GY   T++  +  P
Sbjct: 601 LIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTP 660

Query: 661 FVC--AKNFASTDLNYPSISV--PKLQIG-VPVTVNRRVKNVGSA-GTYVARVRMPKGIT 720
             C   +  +  +LNYPSI+   P  + G V  TV R   NVG A   Y AR+  P+G+T
Sbjct: 661 VRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVT 720

Query: 721 VMVEPSTLQFHSVGEEKAFKLVFHYAQK---VRRPGYVFGALVWSD-GKHFVRSPIAV 755
           V V+P  L F S  + +++ +      +   +   G VFG++ W D GKH VRSPI V
Sbjct: 721 VTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of CmaCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 757/761 (99.47%), Postives = 757/761 (99.47%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS 60
           MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS
Sbjct: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS 60

Query: 61  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 120
           KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG
Sbjct: 61  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 120

Query: 121 SDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSC 180
           SDRGIPWNSIWKASKFGEDVIIGNLDT    ESHSFHDSGYGPVPSRWMGACEGGSNFSC
Sbjct: 121 SDRGIPWNSIWKASKFGEDVIIGNLDTGVWPESHSFHDSGYGPVPSRWMGACEGGSNFSC 180

Query: 181 NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 240
           NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA
Sbjct: 181 NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 240

Query: 241 KGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVG 300
           KGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVG
Sbjct: 241 KGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVG 300

Query: 301 AFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSS 360
           AFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSS
Sbjct: 301 AFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSS 360

Query: 361 LSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDK 420
           LSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDK
Sbjct: 361 LSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDK 420

Query: 421 SFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITP 480
           SFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITP
Sbjct: 421 SFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITP 480

Query: 481 VRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRV 540
           VRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRV
Sbjct: 481 VRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRV 540

Query: 541 PFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKA 600
           PFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKA
Sbjct: 541 PFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKA 600

Query: 601 TPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFAST 660
           TPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFAST
Sbjct: 601 TPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFAST 660

Query: 661 DLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEE 720
           DLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEE
Sbjct: 661 DLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEE 720

Query: 721 KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
           KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 761

BLAST of CmaCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 1434.5 bits (3712), Expect = 0.0e+00
Identity = 719/766 (93.86%), Postives = 734/766 (95.82%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGS-----ESNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLL  SSVHTKKSYIVYLGS      SNPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHASSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWS 120
           TVKGSK+AAKESILYTYNRCINGFAAVLDD EVTALAKNPSVVS+FENRERKLHTTRSW 
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVGSDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGG 180
           FLGV S RGIP NSIWKASKFGEDVIIGNLDT    ES SFHD+GYGPVPSRWMGACEGG
Sbjct: 121 FLGVDSGRGIPQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGG 180

Query: 181 SNFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGN 240
           S F CNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFV+GANVFGN
Sbjct: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGA AQDFA+D
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           +KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGS IETD HMLPTSHVSYIDGLSIA+YLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480

Query: 481 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 540
           A+ITPVRTEIGIKPSPVMA FSSRGPNHI EA+IKPDI+APGVNIIASFT+A AATDLPF
Sbjct: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHI+GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLC QGYNSLTLKKFSNKPFVC+K
Sbjct: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSK 660

Query: 661 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFA TDLNYPSISVPKLQIG PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
            VGEEK FKLVFHYAQ+VRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 GVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766

BLAST of CmaCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)

HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 600/766 (78.33%), Postives = 671/766 (87.60%), Query Frame = 0

Query: 4   SYIISPLLLFFFLLQTSSVHTKKSYIVYLGSES---NPSIYDVQLATESKYDILGTVKGS 63
           SY+ISPLLLFFF+LQT++V T+KSYIVYLGS S   NPSIYDVQLATES+YDILG+VKGS
Sbjct: 8   SYVISPLLLFFFVLQTAAVPTQKSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGS 67

Query: 64  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 123
           KVAAK+SILY+YNR INGFAAVLDD+E TALAKNPSVVSIFEN+ERKLHTTRSWSFLGV 
Sbjct: 68  KVAAKDSILYSYNRYINGFAAVLDDQEATALAKNPSVVSIFENKERKLHTTRSWSFLGVD 127

Query: 124 SDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSC 183
           SDRGIP NSIWKA++FGED IIGNLDT    ES SF+D+GYGPVP+RW GAC+GGS F C
Sbjct: 128 SDRGIPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKFRC 187

Query: 184 NRKLIGARYFYRGYEMINGP---LNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGN 243
           NRKLIGARYFYRG++    P    NIS  +A+DHEGHG+HTLSTAGGNFV G NVFGNGN
Sbjct: 188 NRKLIGARYFYRGFQASERPWTKQNISFDSAQDHEGHGSHTLSTAGGNFVHGVNVFGNGN 247

Query: 244 GTAKGGAPKARVAAYKVCWP--QGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 303
           GTAKGG+P+ARV AYKVCWP   G C D+D+LAGIEAAISDGVDVLS SLG AAQ+FA D
Sbjct: 248 GTAKGGSPRARVVAYKVCWPSENGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHD 307

Query: 304 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 363
            IS+GAFHA+Q GI+VVCSAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNKKQ
Sbjct: 308 AISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQ 367

Query: 364 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 423
           ++GSSLSS  LP GK YPL+ +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG+N
Sbjct: 368 LRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGEN 427

Query: 424 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 483
            R+ K FEV RVGG+GM+LVN++I GS I  D H+LP SH+S  DG+SI +YL STK PV
Sbjct: 428 ARVSKGFEVHRVGGIGMVLVNNQIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPV 487

Query: 484 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 543
           ASIT   TE+GIKPSP+MA FSSRGP+ I EA+IKPDI+APGVNIIAS T  + A+ LPF
Sbjct: 488 ASITHASTEMGIKPSPLMASFSSRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPF 547

Query: 544 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 603
           DKRRVPFN++SGTSMSCPHI+GVAGLLK LHPTWSP AIKSAIMTTAKTRDNTKNT+LD+
Sbjct: 548 DKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTKNTILDY 607

Query: 604 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 663
            KVKATPFDYGAGHVHPN+AMDPGLVYDTT+DDYLNFLC +GYNS TLKKFSNKPFVCAK
Sbjct: 608 TKVKATPFDYGAGHVHPNNAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAK 667

Query: 664 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 723
           NFA+TD NYPSI VP+LQIG  VTVNRRVKNVGSAGTYVARV+MPKGITVMVEPSTLQFH
Sbjct: 668 NFATTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVKMPKGITVMVEPSTLQFH 727

Query: 724 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
           SVGEEK FKLVFHYA K+RR GYVFGALVWSDGKHFVRS IAVNLV
Sbjct: 728 SVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNLV 773

BLAST of CmaCh06G006940 vs. ExPASy TrEMBL
Match: A0A6J1KYS5 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498344 PE=3 SV=1)

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 590/764 (77.23%), Postives = 665/764 (87.04%), Query Frame = 0

Query: 7   ISPLLLFFFLLQTSSVHTKKSYIVYLGSES---NPSIYDVQLATESKYDILGTVKGSKVA 66
           ISPLLLFFF+L T++V TK SYIVYLGS S   NPS+YDVQLATES+YDILG+VKGSKVA
Sbjct: 7   ISPLLLFFFMLHTAAVPTKNSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVA 66

Query: 67  AKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDR 126
           AK+SILY+YNR INGFAAVLD++E TALAKNPSVVS+FEN+ERKLHTTRSW FLGV SDR
Sbjct: 67  AKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDR 126

Query: 127 GIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSCNRK 186
           GIP NSIWKA++FG D IIGNLDT    ES SF+D+GYGPVPSRW GACEGGS F CNRK
Sbjct: 127 GIPQNSIWKAARFGADTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRK 186

Query: 187 LIGARYFYRGYEMINGPL---NISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 246
           LIGARYFYRG++   GPL   NIS  +ARDHEGHG+HTLSTAGGNFV G NVFGNGNGTA
Sbjct: 187 LIGARYFYRGFQAAEGPLTTHNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTA 246

Query: 247 KGGAPKARVAAYKVCWP--QGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPIS 306
           KGG+P+ARV AYKVCWP   G C D+D+LAGIEAAISDGVDVLS S+G  AQ+FA+D IS
Sbjct: 247 KGGSPRARVVAYKVCWPSKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAIS 306

Query: 307 VGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKG 366
           +GAFHA+Q GI+VVCSAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNKK+ +G
Sbjct: 307 IGAFHAVQHGIVVVCSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRFRG 366

Query: 367 SSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRM 426
           SSLSS  LP GK YPL+ +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG+N R+
Sbjct: 367 SSLSSSRLPAGKFYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARV 426

Query: 427 DKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASI 486
            K FEV RVGGVGM+LVN+++ GS +  D H+LP SH+SY DG+SIA+YL STK PVASI
Sbjct: 427 SKGFEVFRVGGVGMVLVNNQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPVASI 486

Query: 487 TPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKR 546
           T   TE+GIKPSP+MA FSSRGP+ I +A+IKPDI+APGVNIIAS T  ++A+ LP DKR
Sbjct: 487 THASTEMGIKPSPLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPLDKR 546

Query: 547 RVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKV 606
           RVPFN++SGTSMSCPHI+GVAGLLK LHPTWSPAAIKSA+MTTAKTRDNTKNT+LD+ KV
Sbjct: 547 RVPFNIESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTKNTLLDYTKV 606

Query: 607 KATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFA 666
           KATPFDYGAGHVHPNDAMDPGLVYDTT+DDYLNFLC +GYNS TLKKFSNKPFVCA NFA
Sbjct: 607 KATPFDYGAGHVHPNDAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANNFA 666

Query: 667 STDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVG 726
           +TD NYPSI VP+LQIG  VTVNRRVKNVGS GTYVARVRMPKGITVMVEPS LQFHSVG
Sbjct: 667 TTDFNYPSILVPRLQIGGSVTVNRRVKNVGSTGTYVARVRMPKGITVMVEPSKLQFHSVG 726

Query: 727 EEKAFKLVFHYAQKVRRP-GYVFGALVWSDGKHFVRSPIAVNLV 758
           EE+ FKL+FHYA K+RR  GYVFGALVWSDGKHFVRS IAVNLV
Sbjct: 727 EERPFKLIFHYAHKLRRQGGYVFGALVWSDGKHFVRSSIAVNLV 770

BLAST of CmaCh06G006940 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 538/740 (72.70%), Postives = 615/740 (83.11%), Query Frame = 0

Query: 27  SYIVYLGSE---SNPSIYDVQLATESKYDILGTVKGSKVAAKESILYTYNRCINGFAAVL 86
           SYIVYLG+    SNP+ YD+++ATES+YD+LG+V GSK+AAK++I Y+YN+ INGFAA L
Sbjct: 6   SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 65

Query: 87  DDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNSIWKASKFGEDVIIG 146
           D+K+   LAKNP VVS+FEN+ERKLHTTRSW FLGV SD GIP NSIW A +FGED IIG
Sbjct: 66  DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 125

Query: 147 NLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMINGPLNI 206
           NLDT    ES SF+D+GYGPVPSRW GACEGG+NF CNRKLIGARYF +G+ M +GPLNI
Sbjct: 126 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI 185

Query: 207 SSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWPQ---GK 266
           S   ARD +GHG+HTLSTAGGNFV GANVFG GNGTAKGG+PKARVAAYKVCWP    G 
Sbjct: 186 SFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGG 245

Query: 267 CSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGNDGP 326
           C DAD+LAG EAAISDGVDVLS+SLG+  ++FA D +S+GAFHA+QQGI+VVCSAGNDGP
Sbjct: 246 CYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGP 305

Query: 327 LPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSVNA 386
            PGTV+N+SPWMFTV ASSIDR F SY  LGNKK  KGSS+SS  L  GK YPL+N+V+A
Sbjct: 306 GPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDA 365

Query: 387 KASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKIS 446
           KA+NAS+ LAQLC +GSLDP KA+GKIIVCLRG+N R++K F VL+ GGVGMILVN K  
Sbjct: 366 KAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNG 425

Query: 447 GSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSSRG 506
           GS    DAH+LP +H+SY DGL++A+Y+ STK PVA ITPV+T++GIKPSPVMA FSSRG
Sbjct: 426 GSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRG 485

Query: 507 PNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGVAG 566
           PN I EAM+KPDI+ PG++I+AS T  V AT  PFD RRVPFNV+SGTSMSCPHI+GV G
Sbjct: 486 PNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVG 545

Query: 567 LLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDPGL 626
           LLK L+PTWSPAAIKSAIMTTAKTRDNT  T+ D  K KATPFDYGAGHVHPN AMDPGL
Sbjct: 546 LLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGL 605

Query: 627 VYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPVTV 686
           VYDTTIDDYLNFLC +GYNSLT K F NKPFVCAK+F  TDLNYPSIS+PKLQ G PVTV
Sbjct: 606 VYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTV 665

Query: 687 NRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGYVF 746
           NRRVKNVG+ GTYVARV     I V VEPSTLQF+SVGEEKAFK+VF Y    +  GYVF
Sbjct: 666 NRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVF 725

Query: 747 GALVWSDGKHFVRSPIAVNL 757
           G L+WSDGKH VRSPI VNL
Sbjct: 726 GTLIWSDGKHNVRSPIVVNL 745

BLAST of CmaCh06G006940 vs. NCBI nr
Match: XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 757/761 (99.47%), Postives = 757/761 (99.47%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS 60
           MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS
Sbjct: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS 60

Query: 61  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 120
           KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG
Sbjct: 61  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 120

Query: 121 SDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSC 180
           SDRGIPWNSIWKASKFGEDVIIGNLDT    ESHSFHDSGYGPVPSRWMGACEGGSNFSC
Sbjct: 121 SDRGIPWNSIWKASKFGEDVIIGNLDTGVWPESHSFHDSGYGPVPSRWMGACEGGSNFSC 180

Query: 181 NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 240
           NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA
Sbjct: 181 NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 240

Query: 241 KGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVG 300
           KGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVG
Sbjct: 241 KGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVG 300

Query: 301 AFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSS 360
           AFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSS
Sbjct: 301 AFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSS 360

Query: 361 LSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDK 420
           LSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDK
Sbjct: 361 LSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDK 420

Query: 421 SFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITP 480
           SFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITP
Sbjct: 421 SFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITP 480

Query: 481 VRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRV 540
           VRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRV
Sbjct: 481 VRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRV 540

Query: 541 PFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKA 600
           PFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKA
Sbjct: 541 PFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKA 600

Query: 601 TPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFAST 660
           TPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFAST
Sbjct: 601 TPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFAST 660

Query: 661 DLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEE 720
           DLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEE
Sbjct: 661 DLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEE 720

Query: 721 KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
           KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 761

BLAST of CmaCh06G006940 vs. NCBI nr
Match: KAG6596783.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1434.9 bits (3713), Expect = 0.0e+00
Identity = 721/766 (94.13%), Postives = 734/766 (95.82%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGS-----ESNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLL TSSVHTKKSYIVYLGS      SNPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHTSSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWS 120
           TVKGSK+AAKESILYTYNRCINGFAAVLDD EVTALAKNPSVVS+FENRERKLHTTRSW 
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVGSDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGG 180
           FLGV S RGI  NSIWKASKFGEDVIIGNLDT    ES SFHD+GYGPVP RWMGACEGG
Sbjct: 121 FLGVDSGRGILQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGG 180

Query: 181 SNFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGN 240
           S F CNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFV+GANVFGN
Sbjct: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGA AQDFA+D
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           +KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGS IETD HMLPTSHVSYIDGLSIA+YLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480

Query: 481 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 540
           A+ITPVRTEIGIKPSPVMA FSSRGPNHI EA+IKPDI+APGVNIIASFT+A AATDLPF
Sbjct: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHI+GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLC QGYNSLTLKKFSNKPFVCAK
Sbjct: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAK 660

Query: 661 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFA TDLNYPSISVPKLQIG PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
           SVGEEK FKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 SVGEEKPFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766

BLAST of CmaCh06G006940 vs. NCBI nr
Match: XP_022949775.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])

HSP 1 Score: 1434.5 bits (3712), Expect = 0.0e+00
Identity = 719/766 (93.86%), Postives = 734/766 (95.82%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGS-----ESNPSIYDVQLATESKYDILG 60
           MEFSYIISPLLLFFFLL  SSVHTKKSYIVYLGS      SNPSIYDVQLATESKYDILG
Sbjct: 1   MEFSYIISPLLLFFFLLHASSVHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILG 60

Query: 61  TVKGSKVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWS 120
           TVKGSK+AAKESILYTYNRCINGFAAVLDD EVTALAKNPSVVS+FENRERKLHTTRSW 
Sbjct: 61  TVKGSKMAAKESILYTYNRCINGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWG 120

Query: 121 FLGVGSDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGG 180
           FLGV S RGIP NSIWKASKFGEDVIIGNLDT    ES SFHD+GYGPVPSRWMGACEGG
Sbjct: 121 FLGVDSGRGIPQNSIWKASKFGEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGG 180

Query: 181 SNFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGN 240
           S F CNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFV+GANVFGN
Sbjct: 181 SKFRCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGN 240

Query: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 300
           GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGA AQDFA+D
Sbjct: 241 GNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFAND 300

Query: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360
           PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ
Sbjct: 301 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 360

Query: 361 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420
           +KGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN
Sbjct: 361 IKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 420

Query: 421 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 480
           GRMDKSFEVLRVGGVGMILVNDKISGS IETD HMLPTSHVSYIDGLSIA+YLKSTKRPV
Sbjct: 421 GRMDKSFEVLRVGGVGMILVNDKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPV 480

Query: 481 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 540
           A+ITPVRTEIGIKPSPVMA FSSRGPNHI EA+IKPDI+APGVNIIASFT+A AATDLPF
Sbjct: 481 ATITPVRTEIGIKPSPVMATFSSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPF 540

Query: 541 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600
           DKRRVPFNVQSGTSMSCPHI+GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 541 DKRRVPFNVQSGTSMSCPHISGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 600

Query: 601 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 660
           NKVKATPFDYGAG VHPN+AMDPGLVYDTTIDDYLNFLC QGYNSLTLKKFSNKPFVC+K
Sbjct: 601 NKVKATPFDYGAGLVHPNNAMDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSK 660

Query: 661 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720
           NFA TDLNYPSISVPKLQIG PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH
Sbjct: 661 NFAITDLNYPSISVPKLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 720

Query: 721 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
            VGEEK FKLVFHYAQ+VRRPGYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 721 GVGEEKPFKLVFHYAQQVRRPGYVFGALVWSDGKHFVRSPIAVNLV 766

BLAST of CmaCh06G006940 vs. NCBI nr
Match: XP_023540421.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 605/766 (78.98%), Postives = 667/766 (87.08%), Query Frame = 0

Query: 4   SYIISPLLLFFFLLQTSSVHTKKSYIVYLGSES---NPSIYDVQLATESKYDILGTVKGS 63
           SY+ISPLLLFFF+LQT +V TKKSYIVYLGS S   NPSIYDVQLATES+Y ILG+VKGS
Sbjct: 8   SYVISPLLLFFFMLQTVAVPTKKSYIVYLGSHSFGPNPSIYDVQLATESQYHILGSVKGS 67

Query: 64  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 123
           KVAAK+SILY+YNR INGFAAVLD++E TALAKNPSVVS+FEN+ERKLHTTRSW FLGV 
Sbjct: 68  KVAAKDSILYSYNRYINGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVD 127

Query: 124 SDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSC 183
           SDRGIP NSIWKA++FGED IIGNLDT    ES SF+D+GYGPVPSRW GACEGGS F C
Sbjct: 128 SDRGIPRNSIWKAARFGEDTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRC 187

Query: 184 NRKLIGARYFYRGYEMINGP---LNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGN 243
           NRKLIGARYFYRG++    P    NIS  +ARDHEGHG+HTLSTAGGNFV G NVFGNGN
Sbjct: 188 NRKLIGARYFYRGFQASERPWTKQNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGN 247

Query: 244 GTAKGGAPKARVAAYKVCWP--QGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADD 303
           GTAKGG+PKARV AYKVCWP   G C D+D+LAGIEAAISDGVDVLS S+G  AQ+FA+D
Sbjct: 248 GTAKGGSPKARVVAYKVCWPSKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFAND 307

Query: 304 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 363
            IS+GAFHA+Q GI+VV SAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNKKQ
Sbjct: 308 AISIGAFHAVQHGIVVVSSAGNDGPSPGSVSNVSPWMITVGASTIDRDFVSYVALGNKKQ 367

Query: 364 VKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDN 423
           ++GSSLSS  LP GK YPLM +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG+N
Sbjct: 368 LRGSSLSSSRLPAGKFYPLMKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGEN 427

Query: 424 GRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPV 483
            R+ K FEVLRVGGVGM+LVN++  GS +  D H+LP SH+SY DG+SIA+YL STK PV
Sbjct: 428 ARLSKGFEVLRVGGVGMVLVNNQTDGSALLADPHILPASHLSYADGVSIAQYLSSTKTPV 487

Query: 484 ASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPF 543
           ASIT   TE+GIKPS VMA FSSRGP  I +A+IKPDI+APGVNIIAS TK V A++LPF
Sbjct: 488 ASITHASTEMGIKPSAVMASFSSRGPELITQAIIKPDITAPGVNIIASVTKDVTASELPF 547

Query: 544 DKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 603
           DKRRV FNV+SGTSMSCPHI+GVAGLLK LHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF
Sbjct: 548 DKRRVSFNVESGTSMSCPHISGVAGLLKTLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF 607

Query: 604 NKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK 663
            KVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLC +GY+SLTLKKFS KPFVCAK
Sbjct: 608 TKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCTRGYSSLTLKKFSKKPFVCAK 667

Query: 664 NFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFH 723
           NF +TD NYPSI VP+LQIG  VTVNRRVKNVGSAGTYVARVRMPKGITVMVEPS LQFH
Sbjct: 668 NFTTTDFNYPSILVPRLQIGESVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSKLQFH 727

Query: 724 SVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
           SVGEEK FKLVFHYA K+RR GYVFGALVWSDGKHFVRS IAVNLV
Sbjct: 728 SVGEEKPFKLVFHYAHKLRRQGYVFGALVWSDGKHFVRSSIAVNLV 773

BLAST of CmaCh06G006940 vs. NCBI nr
Match: KAG6596782.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 599/751 (79.76%), Postives = 661/751 (88.02%), Query Frame = 0

Query: 19  TSSVHTKKSYIVYLGSES---NPSIYDVQLATESKYDILGTVKGSKVAAKESILYTYNRC 78
           T++V TKKSYIVYLGS S   NPSIYDVQLATES+YDILG+VKGSKVAAK+SILY+YNR 
Sbjct: 17  TAAVPTKKSYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRY 76

Query: 79  INGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNSIWKASK 138
           INGFAAVLD++E TALAKNPSVVS+FEN+ERKLHTTRSW FLGV SDRGIP NSIWKA++
Sbjct: 77  INGFAAVLDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPRNSIWKAAR 136

Query: 139 FGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYE 198
           FGED IIGNLDT    ES SF+D+GYGPVPSRW GACEGGS F CN KLIGARYFYRG++
Sbjct: 137 FGEDTIIGNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNSKLIGARYFYRGFQ 196

Query: 199 MINGPL---NISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAY 258
              GPL   NIS ++ARDHEGHG+HTLSTAGGNFV G NVFGNGNGTAKGG+PKARV AY
Sbjct: 197 AAEGPLRTHNISFVSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPKARVVAY 256

Query: 259 KVCWP--QGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGII 318
           KVCWP   G C D+D+LAGIEAAISDGVDVLS S+G  AQ+FA+D IS+GAFHA+Q GI+
Sbjct: 257 KVCWPSKNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIV 316

Query: 319 VVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGK 378
           VV SAGNDGP PG+V+NVSPWM TVGAS+IDR F SYV LGNKKQ++GSSLSS  LP GK
Sbjct: 317 VVSSAGNDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQLRGSSLSSSRLPAGK 376

Query: 379 LYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGV 438
            YPL+ +V  KA+NA+DG AQLC +G+LDP KA+GKIIVCLRG+N R+ K FEVLRVGGV
Sbjct: 377 FYPLIKAVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVLRVGGV 436

Query: 439 GMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPS 498
           GM+LVN++  GS +  D H+LP SH+SY DG+SIA+YL STK PVASIT   TEIGIKPS
Sbjct: 437 GMVLVNNQTDGSALLADPHILPASHISYADGVSIAQYLSSTKTPVASITHASTEIGIKPS 496

Query: 499 PVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSM 558
           P+MA FSSRGP+ I +A+IKPDI+APGVNIIAS TK V A++LPFDKRRV FNV+SGTSM
Sbjct: 497 PLMASFSSRGPDFITQAVIKPDITAPGVNIIASVTKDVTASELPFDKRRVSFNVESGTSM 556

Query: 559 SCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHV 618
           SCPHI+GVAGLLK LHPTWSPAAIKSAIMTTAKTRDNTKNTMLDF KVKATPFDYGAGHV
Sbjct: 557 SCPHISGVAGLLKTLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFTKVKATPFDYGAGHV 616

Query: 619 HPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVP 678
           HPNDAMDPGLVYDTT+DDYLNFLC QGYNS TLKKFSNKPFVCAK FA TDLNYPSISVP
Sbjct: 617 HPNDAMDPGLVYDTTVDDYLNFLCTQGYNSRTLKKFSNKPFVCAKKFAITDLNYPSISVP 676

Query: 679 KLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYA 738
           KLQIG PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPS LQFH+VGEEK FKLVFHYA
Sbjct: 677 KLQIGAPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSKLQFHNVGEEKPFKLVFHYA 736

Query: 739 QKVRRPGYVFGALVWSDGKHFVRSPIAVNLV 758
            K+RR GYVFGALVWSDGKHFVRSPIAVNLV
Sbjct: 737 HKLRRQGYVFGALVWSDGKHFVRSPIAVNLV 767

BLAST of CmaCh06G006940 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 781.9 bits (2018), Expect = 4.5e-226
Identity = 402/764 (52.62%), Postives = 525/764 (68.72%), Query Frame = 0

Query: 11  LLFFFLLQTSSVH-----TKKSYIVYLGSESNPSIYD---VQLATESKYDILGTVKGSKV 70
           LL   L+  SS H        SY+VY G+ S+        +    E+ YD LG+  GS+ 
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 71  AAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSD 130
            A ++I Y+Y + INGFAA LD      ++K+P VVS+F N+  KLHTTRSW FLG+  +
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 131 RGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEG--GSNFSC 190
             +P +SIW+ ++FGED II NLDT    ES SF D G GP+PSRW G C+    + F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189

Query: 191 NRKLIGARYFYRGYEMINGPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTA 250
           NRKLIGARYF +GY    G LN S  + RD +GHG+HTLSTA G+FV G ++FG GNGTA
Sbjct: 190 NRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTA 249

Query: 251 KGGAPKARVAAYKVCWPQGK---CSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPI 310
           KGG+P+ARVAAYKVCWP  K   C DAD+LA  +AAI DG DV+S+SLG     F +D +
Sbjct: 250 KGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSV 309

Query: 311 SVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVK 370
           ++G+FHA ++ I+VVCSAGN GP   TV+NV+PW  TVGAS++DR F S + LGN K  K
Sbjct: 310 AIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYK 369

Query: 371 GSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGR 430
           G SLSS  LP  K YP+M SVNAKA NAS   AQLC+ GSLDP+K +GKI+VCLRG NGR
Sbjct: 370 GQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGR 429

Query: 431 MDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVAS 490
           ++K   V   GG+GM+L N  ++G+D+  D H+LP + ++  D  +++ Y+  TK+P+A 
Sbjct: 430 VEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAH 489

Query: 491 ITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDK 550
           ITP RT++G+KP+PVMA FSS+GP+ +A  ++KPDI+APGV++IA++T AV+ T+  FD 
Sbjct: 490 ITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDP 549

Query: 551 RRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNK 610
           RR+ FN  SGTSMSCPHI+G+AGLLK  +P+WSPAAI+SAIMTTA   D+    + +   
Sbjct: 550 RRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN 609

Query: 611 VKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAK-N 670
           +KATPF +GAGHV PN A++PGLVYD  I DYLNFLC  GYN+  +  FS   F C+   
Sbjct: 610 MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPK 669

Query: 671 FASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHS 730
            +  +LNYPSI+VP L     VTV+R VKNVG    Y  +V  P+G+ V V+P++L F  
Sbjct: 670 ISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTK 729

Query: 731 VGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 757
           VGE+K FK++   ++     GYVFG LVWSD KH VRSPI V L
Sbjct: 730 VGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmaCh06G006940 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 780.0 bits (2013), Expect = 1.7e-225
Identity = 410/762 (53.81%), Postives = 512/762 (67.19%), Query Frame = 0

Query: 7   ISPLLLFFFLLQTSSVHTKKSYIVYLGSESN-PSIYDVQL--ATESKYDILGTVKGSKVA 66
           +S LLL   L  + +   KKSYIVYLGS ++ P I    L     S    L +  GS   
Sbjct: 21  LSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN 80

Query: 67  AKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDR 126
           AKE+I Y+Y R INGFAA+LD+ E   +AK+P VVS+F N+ RKLHTT SW+F+ +  + 
Sbjct: 81  AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 127 GIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFSCNRK 186
            +  +S+W  + +GED II NLDT    ES SF D GYG VP+RW G C    +  CNRK
Sbjct: 141 VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRK 200

Query: 187 LIGARYFYRGYEMING-PLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKG 246
           LIGARYF +GY    G P N S    RDH+GHG+HTLSTA GNFV GANVFG GNGTA G
Sbjct: 201 LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 247 GAPKARVAAYKVCWPQ---GKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISV 306
           G+PKARVAAYKVCWP     +C DAD+LA IEAAI DGVDVLS S+G  A D+  D I++
Sbjct: 261 GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 320

Query: 307 GAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGS 366
           G+FHA++ G+ VVCSAGN GP  GTV+NV+PW+ TVGASS+DR F ++V L N +  KG+
Sbjct: 321 GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 380

Query: 367 SLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMD 426
           SLS   LP  K+Y L+++ +A  +N +   A LC++GSLDP K +GKI+VCLRGDN R+D
Sbjct: 381 SLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVD 440

Query: 427 KSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASIT 486
           K  +    G  GM+L NDK SG++I +DAH+LP S + Y DG ++  YL STK P   I 
Sbjct: 441 KGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIK 500

Query: 487 PVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRR 546
                +  KP+P MA FSSRGPN I   ++KPDI+APGVNIIA+FT+A   TDL  D RR
Sbjct: 501 APTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRR 560

Query: 547 VPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVK 606
            PFN +SGTSMSCPHI+GV GLLK LHP WSPAAI+SAIMTT++TR+N +  M+D +  K
Sbjct: 561 TPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKK 620

Query: 607 ATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKP-FVCAKNFA 666
           A PF YG+GHV PN A  PGLVYD T  DYL+FLC  GYN+  ++ F+  P + C +   
Sbjct: 621 ANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGAN 680

Query: 667 STDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVG 726
             D NYPSI+VP L     +TV R++KNVG   TY AR R P G+ V VEP  L F+  G
Sbjct: 681 LLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 740

Query: 727 EEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVNL 757
           E K F++       V   GYVFG L W+D  H+VRSPI V L
Sbjct: 741 EVKIFQMTLR-PLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmaCh06G006940 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 600.9 bits (1548), Expect = 1.4e-171
Identity = 336/759 (44.27%), Postives = 463/759 (61.00%), Query Frame = 0

Query: 10  LLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGSKVAAKESIL 69
           LL   F   +SS   + +YIV++     PS +D+     + YD         ++    +L
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLH---SNWYD----SSLRSISDSAELL 73

Query: 70  YTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNS 129
           YTY   I+GF+  L  +E  +L   P V+S+      +LHTTR+  FLG+          
Sbjct: 74  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----D 133

Query: 130 IWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFS---CNRKLIG 189
           ++  +    DV++G LDT    ES S+ D G+GP+PS W G CE G+NF+   CNRKLIG
Sbjct: 134 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 193

Query: 190 ARYFYRGYEMINGPLNIS--SLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGA 249
           AR+F RGYE   GP++ S  S + RD +GHGTHT STA G+ V GA++ G  +GTA+G A
Sbjct: 194 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 253

Query: 250 PKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHA 309
           P+ARVA YKVCW  G C  +D+LA I+ AI+D V+VLS+SLG    D+  D +++GAF A
Sbjct: 254 PRARVAVYKVCW-LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 313

Query: 310 IQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSG 369
           +++GI+V CSAGN GP   +++NV+PW+ TVGA ++DR F +   LGN K   G SL  G
Sbjct: 314 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 373

Query: 370 GLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEV 429
                KL P + + N  ASNA++G   LC  G+L P K +GKI++C RG N R+ K   V
Sbjct: 374 EALPDKLLPFIYAGN--ASNATNG--NLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 433

Query: 430 LRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTE 489
              GGVGMIL N   +G ++  DAH+LP + V    G  I  Y+ +   P ASI+ + T 
Sbjct: 434 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTV 493

Query: 490 IGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNV 549
           +G+KPSPV+A FSSRGPN I   ++KPD+ APGVNI+A++T A   T L  D RRV FN+
Sbjct: 494 VGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNI 553

Query: 550 QSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVK-ATPF 609
            SGTSMSCPH++G+A LLK +HP WSPAAI+SA+MTTA         +LD    K +TPF
Sbjct: 554 ISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPF 613

Query: 610 DYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVC--AKNFASTD 669
           D+GAGHV P  A +PGL+YD T +DYL FLC   Y S  ++  S + + C  +K+++  D
Sbjct: 614 DHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVAD 673

Query: 670 LNYPSISVPKLQIGVPVTVNRRVKNVGSAGTYVARVRM-PKGITVMVEPSTLQFHSVGEE 729
           LNYPS +V    +G      R V +VG AGTY  +V     G+ + VEP+ L F    E+
Sbjct: 674 LNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 733

Query: 730 KAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAVN 756
           K++ + F            FG++ WSDGKH V SP+A++
Sbjct: 734 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh06G006940 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 579.7 bits (1493), Expect = 3.4e-165
Identity = 333/778 (42.80%), Postives = 471/778 (60.54%), Query Frame = 0

Query: 1   MEFSYIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGS 60
           M  S I+  L L F  +  ++    K++I  +   S PSI+       S          +
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYS----------T 60

Query: 61  KVAAKESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVG 120
           + A +  I++ Y+   +GF+AV+   E   L  +P+V+++FE+R R+LHTTRS  FLG+ 
Sbjct: 61  EFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ 120

Query: 121 SDRGIPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNFS- 180
           + +G     +W  S +G DVIIG  DT    E  SF D   GP+P RW G CE G+ FS 
Sbjct: 121 NQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSP 180

Query: 181 --CNRKLIGARYFYRGYE-MINGPLN--ISSLNARDHEGHGTHTLSTAGGNFVRGANVFG 240
             CNRK+IGAR+F +G +  + G +N  +  L+ RD +GHGTHT STA G     A++ G
Sbjct: 181 RNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSG 240

Query: 241 NGNGTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGAA---AQD 300
             +G AKG APKAR+AAYKVCW    C D+D+LA  +AA+ DGVDV+SIS+G        
Sbjct: 241 YASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSP 300

Query: 301 FADDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLG 360
           +  DPI++G++ A  +GI V  SAGN+GP   +VTN++PW+ TVGAS+IDR F +   LG
Sbjct: 301 YYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILG 360

Query: 361 NKKQVKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCL 420
           +  +++G SL +G    G+++P++    +  S+AS     LC E +LDP + RGKI++C 
Sbjct: 361 DGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICD 420

Query: 421 RGDNGRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKST 480
           RG + R+ K   V + GGVGMIL N   +G  +  DAH++P   V   +G  I  Y  S 
Sbjct: 421 RGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSH 480

Query: 481 KRPVASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAAT 540
             P+ASI    T +GIKP+PV+A FS RGPN ++  ++KPD+ APGVNI+A++T AV  T
Sbjct: 481 PNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPT 540

Query: 541 DLPFDKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNT 600
            LP D R+  FN+ SGTSM+CPH++G A LLK  HP WSPA I+SA+MTT    DN+  +
Sbjct: 541 GLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRS 600

Query: 601 MLDFNKVK-ATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKP 660
           ++D +  K ATP+DYG+GH++   AM+PGLVYD T DDY+ FLC  GY   T++  +  P
Sbjct: 601 LIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTP 660

Query: 661 FVC--AKNFASTDLNYPSISV--PKLQIG-VPVTVNRRVKNVGSA-GTYVARVRMPKGIT 720
             C   +  +  +LNYPSI+   P  + G V  TV R   NVG A   Y AR+  P+G+T
Sbjct: 661 VRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVT 720

Query: 721 VMVEPSTLQFHSVGEEKAFKLVFHYAQK---VRRPGYVFGALVWSD-GKHFVRSPIAV 755
           V V+P  L F S  + +++ +      +   +   G VFG++ W D GKH VRSPI V
Sbjct: 721 VTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of CmaCh06G006940 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 568.9 bits (1465), Expect = 6.0e-162
Identity = 339/777 (43.63%), Postives = 467/777 (60.10%), Query Frame = 0

Query: 5   YIISPLLLFFFLLQTSSVHTKKSYIVYLGSESNPSIYDVQLATESKYDILGTVKGSKVAA 64
           + + PLLL FF   +SS    +SYIV++     PS++           +L ++  S   A
Sbjct: 10  FFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSH--NNWHVSLLRSLPSSPQPA 69

Query: 65  KESILYTYNRCINGFAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRG 124
             ++LY+Y+R ++GF+A L   +  AL ++PSV+S+  ++ R++HTT + +FLG   + G
Sbjct: 70  --TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 129

Query: 125 IPWNSIWKASKFGEDVIIGNLDT----ESHSFHDSGYGPVPSRWMGACEGGSNF---SCN 184
                +W  S +GEDVI+G LDT    E  SF DSG GP+PS W G CE G +F   SCN
Sbjct: 130 -----LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 189

Query: 185 RKLIGARYFYRGY-EMINGPLNIS---SLNARDHEGHGTHTLSTAGGNFVRGANVFGNGN 244
           RKLIGAR FYRGY    NG    +   S + RD EGHGTHT STA G+ V  A+++    
Sbjct: 190 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 249

Query: 245 GTAKGGAPKARVAAYKVCWPQGKCSDADLLAGIEAAISDGVDVLSISLGA--AAQDFADD 304
           GTA G A KAR+AAYK+CW  G C D+D+LA ++ A++DGV V+S+S+GA  +A ++  D
Sbjct: 250 GTATGMASKARIAAYKICW-TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 309

Query: 305 PISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQ 364
            I++GAF A + GI+V CSAGN GP P T TN++PW+ TVGAS++DR F +    G+ K 
Sbjct: 310 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 369

Query: 365 VKGSSLSSG-GLPRGKLYPLMNSVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGD 424
             G+SL +G  LP  +L             + D  ++LC  G L+     GKI++C RG 
Sbjct: 370 FTGTSLYAGESLPDSQL---------SLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGG 429

Query: 425 NGRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRP 484
           N R++K   V   GG GMIL N   SG ++  D+H++P + V    G  I +Y+K++  P
Sbjct: 430 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSP 489

Query: 485 VASITPVRTEIG-IKPSPVMAPFSSRGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDL 544
            A I+ + T IG   PSP +A FSSRGPNH+   ++KPD+ APGVNI+A +T  V  TDL
Sbjct: 490 TAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDL 549

Query: 545 PFDKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTML 604
             D RRV FN+ SGTSMSCPH++G+A LL+K HP WSPAAIKSA++TTA   +N+   + 
Sbjct: 550 DIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIE 609

Query: 605 DFNKVKAT-PFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFV 664
           D    K++  F +GAGHV PN A++PGLVYD  + +Y+ FLC  GY    +  F   P +
Sbjct: 610 DLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTL 669

Query: 665 -----CAKNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGS--AGTYVARVRMPKGITV 724
                 +K   + DLNYPS SV     G  V   R VKNVGS     Y   V+ P  + +
Sbjct: 670 YDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEI 729

Query: 725 MVEPSTLQF---HSVGE-EKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVRSPIAV 755
            V PS L F    SV E E  FK V         PG+ FG++ W+DG+H V+SP+AV
Sbjct: 730 DVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAV 767