CmaCh06G005680 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G005680
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionserine/arginine-rich SC35-like splicing factor SCL33
LocationCma_Chr06: 2737893 .. 2741132 (+)
RNA-Seq ExpressionCmaCh06G005680
SyntenyCmaCh06G005680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAGACAAAAGCCCCAAGGAAGATTGCAGAATTCTGCTCTCAAGGAAAAGGCTCCGTTCGATTTATCGGTTCTCTGATCCTCAGGTTTTCGCCTTCTTCTTCCCTTTGTTCTTGCTTTCTTTCAATATTTTCTCGATTGCAAATTTGATTTTATCGAGTTTTGATTCAAGAAATCTCTACTTTTTTTTTTTATTATTATTATTCCTTTTTATGCCGAATTGTGTTTTCTATCTCTTTGATCACGTTTGAAGAACGATATCTGTGAAAAAGGCTGCAACTATTGGCGAATTTACATATTTGTCGTTTAACTATTTTTAGAGTAGTTATTCCTTTGAGGTTTTGTTTGATTTGAGAAGCACTCTTGTGAAAGAGAGTTTTGAGAAATCCTGTTTTTGTCTTTGTAGTTTCCTGGTGATATTTTGTTTTCTCCGATATCGATCTCATTCGTTCTGAAGAAATATTGGAGCAGTTGATGGAAGGAAAAGTTTATAAGTTGTCTTTTTGTGACTAGTTTGTCGCTGGATCAATCTGTTATGTTTTTTGTAATTTAGTATCGCTATTGGTAAATTTTCTTACGCTGTTTTTGATTGATCTTCTAGTGGCATATTATTCTTTAAATAGTTGATACCGTGTTTGTGTTCTAAATAAATATTCATCTGCTTGGAACGTTTGCCCGTGTTCAATTGACGCAAAAGAAAGAATATCTCGTTGGATGACAGAAATCTGTGGTAGCATGCTGATTGATGTGCGTTAATGTTCGCAGATTTAGGCTATGTGCATCTTGGTGTTTTTTTTTTTTTTTTTTTTTTTTTAACACCGTGATTTATTTTATTCCAATTGTTATAGGAAATTGAAAATGAGAGGTCGAAGTTACAGTTACAGTCCATCGCCACCAAGGAGTTATGGCAGGAGAAATCGAAGCCTTAGCCCAAGGGGTCATGGAGGCCGTGGAAGGGATTTGCCGACAAGTCTTTTGGTTCGGAACCTAAGGCATGACTGCAGGTTTGTGGCTGCATTTATACAATTATCTGTTGGTTCTTCAATTATGTTTTTGTTGAATTATAAAGAAGTGATATCTTTTTAAAATATGTTCTCTGGTTGATCCAATTTTCAGCTTTCCGTAGTTCTTTCTTTCTTTCATTATTTTAATTGGAAGCTAAGAAGATTCGAATTCAAGTGTTTGTGTAATTTTCTTTGTTGGATTTTTCACCTTTCTCTTGAGCTTGAAGATAAATCAGAATTCTGTTTCAAAAAAAAAAAAAAAAAAAATGGTTTGAAGGTTACAGTTTACCTTTCTCTTGAAAAGCATTTTATATTATATATTCCTTGGGTCGGTTATATTTTGTATTTTCCTTTTCAAAATGAGGAGGAAAAAAACATTTCTGATAGGAAAAAGAACAAAAGCCAACCATTCAAAGAAGACTCTCGACCAAAAAAGATTCCGAGTAATTGCACAATGGTGTTTCAGTTGTTGAGAATTTAAAGGAAAAAAGGATAATGTTTCTGCTTTTGTTTCTATATTGACTTGATACACTTCTTCAATTCTTACGTTTTTTTGCAGGCCAGAGGACCTACGTGGATTATTTGGGCAGTTTGGTCCTCTCAAGGACATCTACTTGCCACGAGATTATTATTCCGGGTAGGCATACTCTTGAAGATTGTGCTTTTTCTTTGTATGAATCTGGTGTGTATGCATGTAAGGGTTTGTCTTACAGTAGTTATAATCAAACTCATTTTAGGCCTTTTATTTCATACGCATGTAGTCTTTATATTTATGAAAATATTTTCTAAATTGTTACTTCTATGGCCTGCTCCTTTGTTCAATTATTTGGTTTCCAAACAACATACAATCAGTTTATCGAGTTTGGTTGTTTTTATTGGAAGTGTTGAAGACTGTCACCAATGAAGTAATAAGATTATCTAGTGGAAGACAAGAAGCAAGTAATTTGGTTGTTGTGTTGAAGACATTCAAGTAAGTTGTGATGACTTAATGTGTACATGAAGATTCGGTGGAAGGTGGGTTTGATTGAATACATTGAGAAGAAGTTCACAAGTCTTGAAGAATTTCATCTCAATTTTTTTCTTTGCCACGCAGAAGGTTAGCATGGTGACACTCACATTTGTTAGTGCTCTATGTTGTCTGTATATGTTTCTCGTCACTGGCAAATTTGTCTCGGGATTGATTGATGATTGTAATGATGCCCCTAGTCATCAAATTGTCGAACTTTGTACAATACATCTATTGTTAAACTTTGTATTTATGAACGGTATGTGATTGTGAGTGCAGAAAACCACGCGGCTTTGGGTTTGTTCAGTACATTGATCCTGCTGATGCTGCTGATGCAAAGTATGAATTAGATGGCCAAGTTCTATTGGGCCGTGAATTAACTGTTGTGTTTGCTGAGGAGAACAGGAAGAGACCTGAACAGATGAGAGCAAGAGACTCATCAAGGTTGCCATTTCTCGACTGCTCTAAACTCTCTACAAATATCTTCAAAATTTTCCCTATAACTTAGTGTTGTTCACATATGAAATTGTTATCAGGGGTCGGTCCTACAGTTACCGGCGTTCCCCACTGCAATATTCTCAATCACCGCCCCATGGTCGAAAATATTCACGTAGTCCTGAATATTATTCCCCTGCTCGGTCTAGACGATATTCAAGGTGGGCAGTTATACTGTAGTATTTTCAATAATGATCATTGGACCATTTAGGAAAAGAGAGGGTTGCGGGGTGGTCGATTCTAACCTTATTTTATATGATACAGGTCAATCTCGCCTAGGGACCAAATGCACAGGGAGCGATCCTACTCAAGGTCACCATATAATTCAAGGGACCGTGGCTATAGCAGAAGCCGAAGCCGAAGCAGGAGTCGGAGCTATAGCCGAAGTTATTCTAGGTGAGTAAAAAATACTTCCAAGTTGCAAAGTGGGTTAAGCTTAGTTAGCTGACTTTTCTAAAAACCCAGAAACTATTAAGGTAGAGAGATGTTTGGATTTCGGAAGTCTGTATATGATTTAGAAAATGCTTTGGTCTTTTCTTTTAGAGCCCACTGATTTCATGTTCTTATTTTAAAATGTATGGACCATGTTGTGGTTTTCTCTATTATGTAGAATGGAACCTTGGGAATTTTAGGCCCTTCTTTTTACCCCCAACACTTCTCTCCAATTTTGTGTCTTACTTCTAGTCTGGTCCTCAATGATTAAAGTGAATATAGTTTAGTGGACTTGACTTTGAC

mRNA sequence

GAGAAGACAAAAGCCCCAAGGAAGATTGCAGAATTCTGCTCTCAAGGAAAAGGCTCCGTTCGATTTATCGGTTCTCTGATCCTCAGTTTCCTGGTGATATTTTGTTTTCTCCGATATCGATCTCATTCGTTCTGAAGAAATATTGGAGCAGTTGATGGAAGGAAAAGTTTATAAGTTGTCTTTTTGTGACTAGTTTGTCGCTGGATCAATCTGTTATGTTTTTTGTAATTTAGTATCGCTATTGAAATCTGTGGTAGCATGCTGATTGATGAAATTGAAAATGAGAGGTCGAAGTTACAGTTACAGTCCATCGCCACCAAGGAGTTATGGCAGGAGAAATCGAAGCCTTAGCCCAAGGGGTCATGGAGGCCGTGGAAGGGATTTGCCGACAAGTCTTTTGGTTCGGAACCTAAGGCATGACTGCAGGCCAGAGGACCTACGTGGATTATTTGGGCAGTTTGGTCCTCTCAAGGACATCTACTTGCCACGAGATTATTATTCCGGAAAACCACGCGGCTTTGGGTTTGTTCAGTACATTGATCCTGCTGATGCTGCTGATGCAAAGTATGAATTAGATGGCCAAGTTCTATTGGGCCGTGAATTAACTGTTGTGTTTGCTGAGGAGAACAGGAAGAGACCTGAACAGATGAGAGCAAGAGACTCATCAAGGGGTCGGTCCTACAGTTACCGGCGTTCCCCACTGCAATATTCTCAATCACCGCCCCATGGTCGAAAATATTCACGTAGTCCTGAATATTATTCCCCTGCTCGGTCTAGACGATATTCAAGGTCAATCTCGCCTAGGGACCAAATGCACAGGGAGCGATCCTACTCAAGGTCACCATATAATTCAAGGGACCGTGGCTATAGCAGAAGCCGAAGCCGAAGCAGGAGTCGGAGCTATAGCCGAAGTTATTCTAGGTGAGTAAAAAATACTTCCAAGTTGCAAAGTGGGTTAAGCTTAGTTAGCTGACTTTTCTAAAAACCCAGAAACTATTAAGGTAGAGAGATGTTTGGATTTCGGAAGTCTGTATATGATTTAGAAAATGCTTTGGTCTTTTCTTTTAGAGCCCACTGATTTCATGTTCTTATTTTAAAATGTATGGACCATGTTGTGGTTTTCTCTATTATGTAGAATGGAACCTTGGGAATTTTAGGCCCTTCTTTTTACCCCCAACACTTCTCTCCAATTTTGTGTCTTACTTCTAGTCTGGTCCTCAATGATTAAAGTGAATATAGTTTAGTGGACTTGACTTTGAC

Coding sequence (CDS)

ATGAAATTGAAAATGAGAGGTCGAAGTTACAGTTACAGTCCATCGCCACCAAGGAGTTATGGCAGGAGAAATCGAAGCCTTAGCCCAAGGGGTCATGGAGGCCGTGGAAGGGATTTGCCGACAAGTCTTTTGGTTCGGAACCTAAGGCATGACTGCAGGCCAGAGGACCTACGTGGATTATTTGGGCAGTTTGGTCCTCTCAAGGACATCTACTTGCCACGAGATTATTATTCCGGAAAACCACGCGGCTTTGGGTTTGTTCAGTACATTGATCCTGCTGATGCTGCTGATGCAAAGTATGAATTAGATGGCCAAGTTCTATTGGGCCGTGAATTAACTGTTGTGTTTGCTGAGGAGAACAGGAAGAGACCTGAACAGATGAGAGCAAGAGACTCATCAAGGGGTCGGTCCTACAGTTACCGGCGTTCCCCACTGCAATATTCTCAATCACCGCCCCATGGTCGAAAATATTCACGTAGTCCTGAATATTATTCCCCTGCTCGGTCTAGACGATATTCAAGGTCAATCTCGCCTAGGGACCAAATGCACAGGGAGCGATCCTACTCAAGGTCACCATATAATTCAAGGGACCGTGGCTATAGCAGAAGCCGAAGCCGAAGCAGGAGTCGGAGCTATAGCCGAAGTTATTCTAGGTGA

Protein sequence

MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Homology
BLAST of CmaCh06G005680 vs. ExPASy Swiss-Prot
Match: Q9SEU4 (Serine/arginine-rich SC35-like splicing factor SCL33 OS=Arabidopsis thaliana OX=3702 GN=SCL33 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.0e-63
Identity = 147/225 (65.33%), Postives = 169/225 (75.11%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRG-HGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQ 64
           MRGR  SY+PSPPR YGRR RS SPRG +GGR RDLPTSLLVRNLRHDCR EDLR  F Q
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 65  FGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKR 124
           FGP+KDIYLPRDYY+G PRGFGFVQ++DPADAADAK+ +DG +LLGRELTVVFAEENRK+
Sbjct: 61  FGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKK 120

Query: 125 PEQMRARDSSRGRSYSYRRSPLQY---SQSPP--HGRKYSRSPEYYSPARSRRYSRSISP 184
           P +MRAR+   GR    RR+P +Y   S+SPP   GR  SRS +YYSP   R + RSISP
Sbjct: 121 PTEMRARERGGGRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYSPPPRRHHPRSISP 180

Query: 185 RDQMH-RERSYSRSPYNSRDRGYS----RSRSRSRSRSYSRSYSR 219
           R++ +   RSYSRSP +   RG S    R +SRS S S  RS SR
Sbjct: 181 REERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPSPRRSISR 223

BLAST of CmaCh06G005680 vs. ExPASy Swiss-Prot
Match: Q9LHP2 (Serine/arginine-rich SC35-like splicing factor SCL30A OS=Arabidopsis thaliana OX=3702 GN=SCL30A PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 2.7e-59
Identity = 144/224 (64.29%), Postives = 167/224 (74.55%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRGH--GGRGRDLPTSLLVRNLRHDCRPEDLRGLFG 64
           MRGR  SY+PSPPR YGRR RS SPRG   G R  DLPTSLLVRNLRHDCR EDLR  F 
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRFGGSRDSDLPTSLLVRNLRHDCRQEDLRRPFE 60

Query: 65  QFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRK 124
           QFGP+KDIYLPRDYY+G PRGFGF+Q++DPADAA+AK+++DG +LLGRELTVVFAEENRK
Sbjct: 61  QFGPVKDIYLPRDYYTGDPRGFGFIQFMDPADAAEAKHQMDGYLLLGRELTVVFAEENRK 120

Query: 125 RPEQMRARDSSRGRSYSY---RRSPLQYSQSPP--HGRK-YSRSPEYYSPARSRRYSRSI 184
           +P +MR RD   GRS  +   RRSP +YS+SPP   GR+  SRS  Y SP   R  SRS+
Sbjct: 121 KPTEMRTRDRG-GRSNRFQDRRRSPPRYSRSPPPRRGRRSRSRSRGYNSPPAKRHQSRSV 180

Query: 185 SPRDQMH-RERSYSRSP--YNSRDRGYSRSRSRSRSRSYSRSYS 218
           SP+D+ + +ERSYSRSP    SR R  S  R +S SRS  RS S
Sbjct: 181 SPQDRRYEKERSYSRSPPHNGSRVRSGSPGRVKSHSRSPRRSVS 221

BLAST of CmaCh06G005680 vs. ExPASy Swiss-Prot
Match: Q1PDV2 (Serine/arginine-rich SC35-like splicing factor SCL28 OS=Arabidopsis thaliana OX=3702 GN=SCL28 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 6.8e-31
Identity = 109/237 (45.99%), Postives = 137/237 (57.81%), Query Frame = 0

Query: 4   KMRGRSYSYSP-----SPPRSY-----GRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCR 63
           + R RS SYSP     SPPR        R     S R H   G   P+ LL+RNL  D R
Sbjct: 3   RARSRSRSYSPRPRDRSPPRERKGYDDNRLRERPSSRDHESSG---PSGLLIRNLPLDAR 62

Query: 64  PEDLRGLFGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELT 123
           P DLR  F +FGPLKDIYLPR+YY+G+PRGFGFV+Y    DAA+A   ++ +V+ GRE+ 
Sbjct: 63  PNDLRDSFERFGPLKDIYLPRNYYTGEPRGFGFVKYRYAEDAAEAMKRMNHKVIGGREIA 122

Query: 124 VVFAEENRKRPEQMRARDSSRGRSYSYR----RSPLQ------YSQSPPHGR-KYS--RS 183
           +VFAEENRK P++MR  + + GR   Y+    RSP +       S+SPP    ++S  R 
Sbjct: 123 IVFAEENRKTPQEMRTTNGTSGRHGDYKRTSHRSPRRRYRSHSRSRSPPRRESRHSKVRE 182

Query: 184 PEYYSP-ARSRRYSRSISPRDQMHRERSYSRSPYNSRDRGYSRSR--SRSRSRSYSR 215
            + YSP  RSR  SRS  PR++   +    RSP   R     RSR  SRSRSRS SR
Sbjct: 183 DDLYSPRRRSRSISRSPLPRNEREYKSRNCRSPREERVLTPIRSRCLSRSRSRSLSR 236

BLAST of CmaCh06G005680 vs. ExPASy Swiss-Prot
Match: Q8L3X8 (Serine/arginine-rich SC35-like splicing factor SCL30 OS=Arabidopsis thaliana OX=3702 GN=SCL30 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.2e-30
Identity = 97/222 (43.69%), Postives = 122/222 (54.95%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSP----RGHGGRG------RDLPTSLLVRNLRHDCRP 64
           MR  S  Y   P R YG R RS  P    RG+GG G           SLLVRN+  DCRP
Sbjct: 1   MRRYSPPYYSPPRRGYGGRGRSPPPPPPRRGYGGGGGGGGRRGSSHGSLLVRNIPLDCRP 60

Query: 65  EDLRGLFGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTV 124
           E+LR  F +FGP++D+Y+PRDYYSG+PRGF FV+++D  DA +A+  ++ +   GRE+TV
Sbjct: 61  EELREPFERFGPVRDVYIPRDYYSGQPRGFAFVEFVDAYDAGEAQRSMNRRSFAGREITV 120

Query: 125 VFAEENRKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSR 184
           V A E+RKRPE+MR +  +R R  S  R    + +S       SRSP   S +RSR  SR
Sbjct: 121 VVASESRKRPEEMRVKTRTRSREPSGSRD-RSHGRSRSRSISRSRSPRRPSDSRSRYRSR 180

Query: 185 SISPRDQMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSY 217
           S SP       R     P    D  YSR       RSYS  Y
Sbjct: 181 SYSP-----APRRRGGPPRGEEDENYSR-------RSYSPGY 209

BLAST of CmaCh06G005680 vs. ExPASy Swiss-Prot
Match: O75494 (Serine/arginine-rich splicing factor 10 OS=Homo sapiens OX=9606 GN=SRSF10 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 87/195 (44.62%), Postives = 118/195 (60.51%), Query Frame = 0

Query: 41  TSLLVRNLRHDCRPEDLRGLFGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKY 100
           TSL VRN+  D R EDLR  FG++GP+ D+Y+P D+Y+ +PRGF +VQ+ D  DA DA +
Sbjct: 10  TSLFVRNVADDTRSEDLRREFGRYGPIVDVYVPLDFYTRRPRGFAYVQFEDVRDAEDALH 69

Query: 101 ELDGQVLLGRELTVVFAEENRKRPEQMRARD------SSRGRSYS-YRRSPLQYSQSPPH 160
            LD + + GR++ + FA+ +RK P QM+A++      SSR   Y  YRRS    S+S   
Sbjct: 70  NLDRKWICGRQIEIQFAQGDRKTPNQMKAKEGRNVYSSSRYDDYDRYRRS---RSRSYER 129

Query: 161 GRKYSRSPEY-----YSPARSR-----RYSRSISPRDQM-HRERSYSRSPYNSRDRGYSR 218
            R  SRS +Y     YSP  SR     R SRS S  D+  HR RS+SRS  NSR R  S+
Sbjct: 130 RRSRSRSFDYNYRRSYSPRNSRPTGRPRRSRSHSDNDRFKHRNRSFSRSKSNSRSRSKSQ 189

BLAST of CmaCh06G005680 vs. ExPASy TrEMBL
Match: A0A6J1L0E4 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498719 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.1e-115
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL 60
           MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL
Sbjct: 1   MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL 60

Query: 61  FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN 120
           FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN
Sbjct: 61  FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN 120

Query: 121 RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD 180
           RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD
Sbjct: 121 RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD 180

Query: 181 QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 181 QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 218

BLAST of CmaCh06G005680 vs. ExPASy TrEMBL
Match: A0A6J1L3A1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498719 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.3e-114
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 2   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 61
           KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 8   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 67

Query: 62  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 121
           GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR
Sbjct: 68  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 127

Query: 122 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 181
           KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ
Sbjct: 128 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 187

Query: 182 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 188 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 224

BLAST of CmaCh06G005680 vs. ExPASy TrEMBL
Match: A0A6J1KYI3 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111498719 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.4e-113
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQF 64
           MRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQF
Sbjct: 1   MRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQF 60

Query: 65  GPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRP 124
           GPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRP
Sbjct: 61  GPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRP 120

Query: 125 EQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHR 184
           EQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHR
Sbjct: 121 EQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHR 180

Query: 185 ERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           ERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 181 ERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 214

BLAST of CmaCh06G005680 vs. ExPASy TrEMBL
Match: A0A6J1G9D2 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452132 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.6e-107
Identity = 207/218 (94.95%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL 60
           MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL
Sbjct: 1   MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL 60

Query: 61  FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN 120
           FG+FGPLKDIYLPRDYYSGKPRGFGFVQYIDP+DAADAKYELDGQVLLGRELTVVFAEEN
Sbjct: 61  FGRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEEN 120

Query: 121 RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD 180
           RKRPEQMRARDSS GRSYSYRRSPLQYSQSPPHGRKYS SPEYYSPARS RYSRSISPRD
Sbjct: 121 RKRPEQMRARDSSSGRSYSYRRSPLQYSQSPPHGRKYSPSPEYYSPARSGRYSRSISPRD 180

Query: 181 QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           + +RERSYSRSPY+SRDRGY  SRSRSRSRSYSRSYSR
Sbjct: 181 RRYRERSYSRSPYDSRDRGY--SRSRSRSRSYSRSYSR 216

BLAST of CmaCh06G005680 vs. ExPASy TrEMBL
Match: A0A6J1G9Y3 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452132 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.4e-106
Identity = 206/217 (94.93%), Postives = 211/217 (97.24%), Query Frame = 0

Query: 2   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 61
           KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 8   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 67

Query: 62  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 121
           G+FGPLKDIYLPRDYYSGKPRGFGFVQYIDP+DAADAKYELDGQVLLGRELTVVFAEENR
Sbjct: 68  GRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEENR 127

Query: 122 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 181
           KRPEQMRARDSS GRSYSYRRSPLQYSQSPPHGRKYS SPEYYSPARS RYSRSISPRD+
Sbjct: 128 KRPEQMRARDSSSGRSYSYRRSPLQYSQSPPHGRKYSPSPEYYSPARSGRYSRSISPRDR 187

Query: 182 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
            +RERSYSRSPY+SRDRGY  SRSRSRSRSYSRSYSR
Sbjct: 188 RYRERSYSRSPYDSRDRGY--SRSRSRSRSYSRSYSR 222

BLAST of CmaCh06G005680 vs. NCBI nr
Match: XP_023005834.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 [Cucurbita maxima])

HSP 1 Score: 423.3 bits (1087), Expect = 1.3e-114
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL 60
           MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL
Sbjct: 1   MKLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGL 60

Query: 61  FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN 120
           FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN
Sbjct: 61  FGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEEN 120

Query: 121 RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD 180
           RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD
Sbjct: 121 RKRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD 180

Query: 181 QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 181 QMHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 218

BLAST of CmaCh06G005680 vs. NCBI nr
Match: XP_023005833.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita maxima])

HSP 1 Score: 421.4 bits (1082), Expect = 4.8e-114
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 2   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 61
           KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 8   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 67

Query: 62  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 121
           GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR
Sbjct: 68  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 127

Query: 122 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 181
           KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ
Sbjct: 128 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 187

Query: 182 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 188 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 224

BLAST of CmaCh06G005680 vs. NCBI nr
Match: XP_023005835.1 (serine/arginine-rich SC35-like splicing factor SCL33 isoform X3 [Cucurbita maxima])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQF 64
           MRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQF
Sbjct: 1   MRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQF 60

Query: 65  GPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRP 124
           GPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRP
Sbjct: 61  GPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKRP 120

Query: 125 EQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHR 184
           EQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHR
Sbjct: 121 EQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQMHR 180

Query: 185 ERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
           ERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 181 ERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 214

BLAST of CmaCh06G005680 vs. NCBI nr
Match: KAG6596659.1 (Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 410.6 bits (1054), Expect = 8.4e-111
Identity = 211/217 (97.24%), Postives = 216/217 (99.54%), Query Frame = 0

Query: 2   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 61
           KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 45  KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 104

Query: 62  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 121
           G+FGPLKDIYLPRDYYSGKPRGFGFVQYIDP+DAADAKYELDGQVLLGRELTVVFAEENR
Sbjct: 105 GRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPSDAADAKYELDGQVLLGRELTVVFAEENR 164

Query: 122 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 181
           KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD+
Sbjct: 165 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDR 224

Query: 182 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
            +RERSYSRSPY+SRDRGYSRSRSRSRSRSYSRSYSR
Sbjct: 225 RYRERSYSRSPYDSRDRGYSRSRSRSRSRSYSRSYSR 261

BLAST of CmaCh06G005680 vs. NCBI nr
Match: XP_023539101.1 (serine/arginine-rich SC35-like splicing factor SCL33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 398.7 bits (1023), Expect = 3.3e-107
Identity = 208/217 (95.85%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 2   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 61
           KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF
Sbjct: 8   KLKMRGRSYSYSPSPPRSYGRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCRPEDLRGLF 67

Query: 62  GQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 121
           G+FGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR
Sbjct: 68  GRFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENR 127

Query: 122 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDQ 181
           KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRD+
Sbjct: 128 KRPEQMRARDSSRGRSYSYRRSPLQYSQSPPHGRKYSRSPEYYSPARSRRYSRSISPRDR 187

Query: 182 MHRERSYSRSPYNSRDRGYSRSRSRSRSRSYSRSYSR 219
            +RE SYSRSPY+SRDRGY  SRSRSRSRSYS SYSR
Sbjct: 188 RYREISYSRSPYDSRDRGY--SRSRSRSRSYSGSYSR 222

BLAST of CmaCh06G005680 vs. TAIR 10
Match: AT1G55310.2 (SC35-like splicing factor 33 )

HSP 1 Score: 243.4 bits (620), Expect = 1.7e-64
Identity = 142/216 (65.74%), Postives = 165/216 (76.39%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRG-HGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQ 64
           MRGR  SY+PSPPR YGRR RS SPRG +GGR RDLPTSLLVRNLRHDCR EDLR  F Q
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 65  FGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKR 124
           FGP+KDIYLPRDYY+G PRGFGFVQ++DPADAADAK+ +DG +LLGRELTVVFAEENRK+
Sbjct: 61  FGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKK 120

Query: 125 PEQMRARDSSRGRSYSYRRSPLQY---SQSPP--HGRKYSRSPEYYSPARSRRYSRSISP 184
           P +MRAR+   GR    RR+P +Y   S+SPP   GR  SRS +YYSP   R + RSISP
Sbjct: 121 PTEMRARERGGGRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYSPPPRRHHPRSISP 180

Query: 185 RDQMH-RERSYSRSPYNSRDRGYSRSRSRSRSRSYS 214
           R++ +   RSYSRSP +   RG S +  R +SRS S
Sbjct: 181 REERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLS 214

BLAST of CmaCh06G005680 vs. TAIR 10
Match: AT1G55310.1 (SC35-like splicing factor 33 )

HSP 1 Score: 242.7 bits (618), Expect = 2.8e-64
Identity = 147/225 (65.33%), Postives = 169/225 (75.11%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRG-HGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQ 64
           MRGR  SY+PSPPR YGRR RS SPRG +GGR RDLPTSLLVRNLRHDCR EDLR  F Q
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 65  FGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRKR 124
           FGP+KDIYLPRDYY+G PRGFGFVQ++DPADAADAK+ +DG +LLGRELTVVFAEENRK+
Sbjct: 61  FGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHHMDGYLLLGRELTVVFAEENRKK 120

Query: 125 PEQMRARDSSRGRSYSYRRSPLQY---SQSPP--HGRKYSRSPEYYSPARSRRYSRSISP 184
           P +MRAR+   GR    RR+P +Y   S+SPP   GR  SRS +YYSP   R + RSISP
Sbjct: 121 PTEMRARERGGGRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYSPPPRRHHPRSISP 180

Query: 185 RDQMH-RERSYSRSPYNSRDRGYS----RSRSRSRSRSYSRSYSR 219
           R++ +   RSYSRSP +   RG S    R +SRS S S  RS SR
Sbjct: 181 REERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPSPRRSISR 223

BLAST of CmaCh06G005680 vs. TAIR 10
Match: AT3G13570.1 (SC35-like splicing factor 30A )

HSP 1 Score: 229.9 bits (585), Expect = 1.9e-60
Identity = 144/224 (64.29%), Postives = 167/224 (74.55%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRGH--GGRGRDLPTSLLVRNLRHDCRPEDLRGLFG 64
           MRGR  SY+PSPPR YGRR RS SPRG   G R  DLPTSLLVRNLRHDCR EDLR  F 
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRFGGSRDSDLPTSLLVRNLRHDCRQEDLRRPFE 60

Query: 65  QFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELTVVFAEENRK 124
           QFGP+KDIYLPRDYY+G PRGFGF+Q++DPADAA+AK+++DG +LLGRELTVVFAEENRK
Sbjct: 61  QFGPVKDIYLPRDYYTGDPRGFGFIQFMDPADAAEAKHQMDGYLLLGRELTVVFAEENRK 120

Query: 125 RPEQMRARDSSRGRSYSY---RRSPLQYSQSPP--HGRK-YSRSPEYYSPARSRRYSRSI 184
           +P +MR RD   GRS  +   RRSP +YS+SPP   GR+  SRS  Y SP   R  SRS+
Sbjct: 121 KPTEMRTRDRG-GRSNRFQDRRRSPPRYSRSPPPRRGRRSRSRSRGYNSPPAKRHQSRSV 180

Query: 185 SPRDQMH-RERSYSRSP--YNSRDRGYSRSRSRSRSRSYSRSYS 218
           SP+D+ + +ERSYSRSP    SR R  S  R +S SRS  RS S
Sbjct: 181 SPQDRRYEKERSYSRSPPHNGSRVRSGSPGRVKSHSRSPRRSVS 221

BLAST of CmaCh06G005680 vs. TAIR 10
Match: AT1G55310.3 (SC35-like splicing factor 33 )

HSP 1 Score: 229.6 bits (584), Expect = 2.5e-60
Identity = 147/238 (61.76%), Postives = 169/238 (71.01%), Query Frame = 0

Query: 5   MRGRSYSYSPSPPRSYGRRNRSLSPRG-HGGRGRDLPTSLLVRNLRHDCRPEDLRGLFGQ 64
           MRGR  SY+PSPPR YGRR RS SPRG +GGR RDLPTSLLVRNLRHDCR EDLR  F Q
Sbjct: 1   MRGR--SYTPSPPRGYGRRGRSPSPRGRYGGRSRDLPTSLLVRNLRHDCRQEDLRKSFEQ 60

Query: 65  FGPLKDIYLPRDYYSGK------------PRGFGFVQYIDPADAADAKYELDGQVLLGRE 124
           FGP+KDIYLPRDYY+G             PRGFGFVQ++DPADAADAK+ +DG +LLGRE
Sbjct: 61  FGPVKDIYLPRDYYTGSHLCTDTCKASRDPRGFGFVQFMDPADAADAKHHMDGYLLLGRE 120

Query: 125 LTVVFAEENRKRPEQMRARDSSRG-RSYSYRRSPLQY---SQSPP--HGRKYSRSPEYYS 184
           LTVVFAEENRK+P +MRAR+   G R    RR+P +Y   S+SPP   GR  SRS +YYS
Sbjct: 121 LTVVFAEENRKKPTEMRARERGGGSRFRDRRRTPPRYYSRSRSPPPRRGRSRSRSGDYYS 180

Query: 185 PARSRRYSRSISPRDQMH-RERSYSRSPYNSRDRGYS----RSRSRSRSRSYSRSYSR 219
           P   R + RSISPR++ +   RSYSRSP +   RG S    R +SRS S S  RS SR
Sbjct: 181 PPPRRHHPRSISPREERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPSPRRSISR 236

BLAST of CmaCh06G005680 vs. TAIR 10
Match: AT5G18810.1 (SC35-like splicing factor 28 )

HSP 1 Score: 135.6 bits (340), Expect = 4.9e-32
Identity = 109/237 (45.99%), Postives = 137/237 (57.81%), Query Frame = 0

Query: 4   KMRGRSYSYSP-----SPPRSY-----GRRNRSLSPRGHGGRGRDLPTSLLVRNLRHDCR 63
           + R RS SYSP     SPPR        R     S R H   G   P+ LL+RNL  D R
Sbjct: 3   RARSRSRSYSPRPRDRSPPRERKGYDDNRLRERPSSRDHESSG---PSGLLIRNLPLDAR 62

Query: 64  PEDLRGLFGQFGPLKDIYLPRDYYSGKPRGFGFVQYIDPADAADAKYELDGQVLLGRELT 123
           P DLR  F +FGPLKDIYLPR+YY+G+PRGFGFV+Y    DAA+A   ++ +V+ GRE+ 
Sbjct: 63  PNDLRDSFERFGPLKDIYLPRNYYTGEPRGFGFVKYRYAEDAAEAMKRMNHKVIGGREIA 122

Query: 124 VVFAEENRKRPEQMRARDSSRGRSYSYR----RSPLQ------YSQSPPHGR-KYS--RS 183
           +VFAEENRK P++MR  + + GR   Y+    RSP +       S+SPP    ++S  R 
Sbjct: 123 IVFAEENRKTPQEMRTTNGTSGRHGDYKRTSHRSPRRRYRSHSRSRSPPRRESRHSKVRE 182

Query: 184 PEYYSP-ARSRRYSRSISPRDQMHRERSYSRSPYNSRDRGYSRSR--SRSRSRSYSR 215
            + YSP  RSR  SRS  PR++   +    RSP   R     RSR  SRSRSRS SR
Sbjct: 183 DDLYSPRRRSRSISRSPLPRNEREYKSRNCRSPREERVLTPIRSRCLSRSRSRSLSR 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SEU44.0e-6365.33Serine/arginine-rich SC35-like splicing factor SCL33 OS=Arabidopsis thaliana OX=... [more]
Q9LHP22.7e-5964.29Serine/arginine-rich SC35-like splicing factor SCL30A OS=Arabidopsis thaliana OX... [more]
Q1PDV26.8e-3145.99Serine/arginine-rich SC35-like splicing factor SCL28 OS=Arabidopsis thaliana OX=... [more]
Q8L3X81.2e-3043.69Serine/arginine-rich SC35-like splicing factor SCL30 OS=Arabidopsis thaliana OX=... [more]
O754941.9e-2544.62Serine/arginine-rich splicing factor 10 OS=Homo sapiens OX=9606 GN=SRSF10 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1L0E46.1e-115100.00serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita max... [more]
A0A6J1L3A12.3e-114100.00serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita max... [more]
A0A6J1KYI37.4e-113100.00serine/arginine-rich SC35-like splicing factor SCL33 isoform X3 OS=Cucurbita max... [more]
A0A6J1G9D23.6e-10794.95serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 OS=Cucurbita mos... [more]
A0A6J1G9Y31.4e-10694.93serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 OS=Cucurbita mos... [more]
Match NameE-valueIdentityDescription
XP_023005834.11.3e-114100.00serine/arginine-rich SC35-like splicing factor SCL33 isoform X2 [Cucurbita maxim... [more]
XP_023005833.14.8e-114100.00serine/arginine-rich SC35-like splicing factor SCL33 isoform X1 [Cucurbita maxim... [more]
XP_023005835.11.5e-112100.00serine/arginine-rich SC35-like splicing factor SCL33 isoform X3 [Cucurbita maxim... [more]
KAG6596659.18.4e-11197.24Serine/arginine-rich SC35-like splicing factor SCL30A, partial [Cucurbita argyro... [more]
XP_023539101.13.3e-10795.85serine/arginine-rich SC35-like splicing factor SCL33 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
AT1G55310.21.7e-6465.74SC35-like splicing factor 33 [more]
AT1G55310.12.8e-6465.33SC35-like splicing factor 33 [more]
AT3G13570.11.9e-6064.29SC35-like splicing factor 30A [more]
AT1G55310.32.5e-6061.76SC35-like splicing factor 33 [more]
AT5G18810.14.9e-3245.99SC35-like splicing factor 28 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 42..115
e-value: 7.4E-23
score: 91.9
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 43..112
e-value: 6.9E-20
score: 70.7
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 41..119
score: 17.642843
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 36..146
e-value: 3.1E-26
score: 94.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 121..218
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 121..137
NoneNo IPR availablePANTHERPTHR23147:SF155SERINE/ARGININE-RICH SC35-LIKE SPLICING FACTOR SCL30Acoord: 5..218
NoneNo IPR availablePANTHERPTHR23147SERINE/ARGININE RICH SPLICING FACTORcoord: 5..218
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 41..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G005680.1CmaCh06G005680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016607 nuclear speck
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding