CmaCh06G005270 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G005270 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 9.9e-124
Identity = 236/435 (54.25%), Postives = 309/435 (71.03%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDS 61
           +EIE+P++FLCPISL IMKDPV V +GITYDR SIE WLFS K  SCP+TK  I      
Sbjct: 3   QEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVI--TETD 62

Query: 62  LTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLR 121
           LTPNHTLRRLIQ+WCTL++ +GIER PTPKPPI K +I KLI  SSS+  + + C++RLR
Sbjct: 63  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 122

Query: 122 SLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALIS------------------- 181
            + +E+ +N+RC+E+A  PEFLA+++ NS+   ++ ++ +S                   
Sbjct: 123 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 182

Query: 182 ----CHEALSTLHNLRLSDSKLKSLTVN---AELFDSLTKVMEQGTYESRAYAALLLKSL 241
                 EALS L++L  S++ LKSL  N     L  +LTK+M++G YESRAYAALLLK L
Sbjct: 183 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 242

Query: 242 LEVADPMELTFLKSELFVEIVGILKDQTSQQSLKAALGILITACTWGRNKVKAVEAGGVV 301
           LEVADPM++  L+ ELF E++ IL DQ S ++ ++A+ IL+  C WGRN+ KAVE G + 
Sbjct: 243 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 302

Query: 302 ALVEILLS---GPERRVSEMALTAMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGS 361
            ++E+L+      ERR SEMA+  +D+LC CAEGRA  L HG  +AVVSKKILRVSQ+ S
Sbjct: 303 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 362

Query: 362 ERAVRILFSVAKYSGSPAVLTEMAQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWR 408
           ERAVR+L SV ++  +P++L EM QLG+VAKLCLVLQV  G+KTKEKAKE+LK+HARVWR
Sbjct: 363 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWR 422

BLAST of CmaCh06G005270 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.4e-122
Identity = 230/411 (55.96%), Postives = 307/411 (74.70%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDS 61
           EEIE+P +FLCPISL+IMKDPV V +GITYDR SIE WLF+ K  SCP+TK  I  D+D 
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI-TDAD- 67

Query: 62  LTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLR 121
           LTPNHTLRRLIQ+WCTL++ +G+ER PTP+PPI K +I KLI  S+S+  + + C++RLR
Sbjct: 68  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 127

Query: 122 SLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKLK 181
            + +E+ +N+RC+E+AG PEFLA+++      ND+E   ++  EAL+ L++L  S++ LK
Sbjct: 128 QIVSENATNKRCLEAAGVPEFLANIV-----SNDSENGSLT-DEALNLLYHLETSETVLK 187

Query: 182 SLTVNAE---LFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGI 241
           +L  N +   +  SLTK+M++G YESR YA LLLK++LEVADPM+   LK E+F E+V I
Sbjct: 188 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQI 247

Query: 242 LKDQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLS---GPERRVSEMALT 301
           L D+ SQ++ KAA+ IL+  C WGRN+ KAVEAG +  ++E+L+      ERR  EMA+ 
Sbjct: 248 LDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMV 307

Query: 302 AMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEM 361
            +DLLC CAEGRA  L HG  +AVV KKILRVSQ  S+RAVR+L SV ++  +PA+L EM
Sbjct: 308 VLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEM 367

Query: 362 AQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYP 407
            QLG+VAKLCLVLQV  G KTKEKAKE+LK+HARVW++S C+P  +  +YP
Sbjct: 368 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of CmaCh06G005270 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.0e-67
Identity = 170/437 (38.90%), Postives = 241/437 (55.15%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDS 61
           EEIE+PNYF+CPISL+IMKDPVT  SGITYDR +I  WL   K  SCP+TK P+P DSD 
Sbjct: 6   EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD- 65

Query: 62  LTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLL---SCIR 121
           LTPNH LRRLIQ WC  +   G+ R  TP+ P  K  + + I +        L     ++
Sbjct: 66  LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQ 125

Query: 122 RLRSLAAESESNRRCIESAGAPEFLASVIINSIS-DNDNETALISCHEALSTLHNLRLSD 181
           +L  LA +  +NRR +   G  + L   ++   S D D    +    E+L  LH + +  
Sbjct: 126 KLEVLAMDG-NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 185

Query: 182 SKLKSLTV-NAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIV 241
           +  K++ + N  + +SLT V+ Q  + S+AY  +LL++L E      +  L  E+F  I+
Sbjct: 186 NDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGII 245

Query: 242 GILKD----------------QTS-------------------QQSLKAALGILITACTW 301
           G LKD                Q+S                   +Q++ AAL IL+   +W
Sbjct: 246 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 305

Query: 302 GRNKVKAVEAGGVVALVEILLS-GPERRVSEMALTAMDLLCGCAEGRAALLGHGGGMAVV 361
            RN+   V+ G V  L+E+ +S   E+R++E+ L  +  LC CA GRA +L H GG+AVV
Sbjct: 306 SRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVV 365

Query: 362 SKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGIVAKLCLVLQVESGSKTKEKA 398
           +K++LRVS    +RA+ IL +V+K+S    V+ EM  +G V KLC VL ++ G   KEKA
Sbjct: 366 TKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNLKEKA 425

BLAST of CmaCh06G005270 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.7e-47
Identity = 129/406 (31.77%), Postives = 221/406 (54.43%), Query Frame = 0

Query: 4   IEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDSLT 63
           I++P +F CPISL +M DPVT+ +G TYDR SI++W+ +  N +CP+T++ +     +L 
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVAL--SDFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLS------CI 123
           PNHTLRRLIQ WC  +  +G+ER PTPK P +   +  L+S +S+   + +S       I
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAI 131

Query: 124 RRLRSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSD 183
           RRLR LA +SE NR  I    A E L  ++   I      + L+S  E+L+ L  L +++
Sbjct: 132 RRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVS--ESLALLVLLHMTE 191

Query: 184 SKLKSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKS---ELFVE 243
           ++ +++  +      +T+++   + E R  AA L++ +L  A  M+L  + S    +F  
Sbjct: 192 TECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 244 IVGILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMA 303
           ++ +LK+  +S+++LK  +  +   C   + +  A+ AG    L++ L +  +R  +E  
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTERG 311

Query: 304 LTAMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLT 363
           L  ++LLC   EG AA   H   + ++ K ILRVS   +E A   L ++   +       
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEERCRD 371

Query: 364 EMAQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPS 400
           E A  G+V +L L++Q +   + K KA+ +LK+    W + + + S
Sbjct: 372 EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of CmaCh06G005270 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.2e-44
Identity = 128/399 (32.08%), Postives = 221/399 (55.39%), Query Frame = 0

Query: 4   IEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDSLT 63
           I++P +F CPISL++M+DPVTV +G TYDR SIE+W+    N +CP+T+ P+     +L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL--SDFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLS------CI 123
           PNHTLRRLIQ WC  +  +G+ER PTPK P +   +  L+S +S+   + +S       +
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAAL 131

Query: 124 RRLRSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSD 183
           RRLR  A +S+ NR  I +  A E L  ++ +  + ++    L+S  E+L+ L  L +++
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSE----LVS--ESLALLVMLPITE 191

Query: 184 -SKLKSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSL---LEVADPMELTFLKSELFV 243
            ++  S++ +    + LT+++   + E+R  AA L++ +    + AD          +F 
Sbjct: 192 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFE 251

Query: 244 EIVGILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEM 303
            ++ +L++  +S+++LK  +  L   C+    +  A+ AG    L++ L +  +R  +E 
Sbjct: 252 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 311

Query: 304 ALTAMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVL 363
           AL  ++LLC   EG AA   H   + ++ K ILRVS   +E A   L ++   +      
Sbjct: 312 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWR 371

Query: 364 TEMAQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVW 392
            E A  G+V +L L++Q E   + K+KA+++LK+    W
Sbjct: 372 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of CmaCh06G005270 vs. ExPASy TrEMBL
Match: A0A6J1KXX6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498577 PE=4 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 2.8e-222
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. ExPASy TrEMBL
Match: A0A6J1GKE4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455050 PE=4 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.5e-220
Identity = 405/408 (99.26%), Postives = 405/408 (99.26%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFS KNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSNKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETA ISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALL KSLLEVADPMELTFLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLFKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. ExPASy TrEMBL
Match: A0A5D3DNW0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004610 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.4e-160
Identity = 308/412 (74.76%), Postives = 357/412 (86.65%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIP-ADSD 61
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCPITKLP+  +DSD
Sbjct: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSDSD 62

Query: 62  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121
            LTPNHTLRRLIQAWCTL+S +G+ERFPTPKPPI+K QI  +IS+S+++PSSL+S IRRL
Sbjct: 63  LLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIRRL 122

Query: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 181
           RS++AESE+NRRC+E AGAPEFL SVI++S S         + HEALSTLHNLRLSDS  
Sbjct: 123 RSISAESETNRRCVEFAGAPEFLVSVIVDSDSS--------ASHEALSTLHNLRLSDSTF 182

Query: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLLKSLLEVADPMELTFLKSELFVEIVG 241
           KSL  + E+ +SLT  M  +QGT+E SR YA L+LKS++EVADP++L+FLK ELFV+IV 
Sbjct: 183 KSLATHPEVLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVE 242

Query: 242 ILKDQTSQ-QSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTA 301
           ILKD++S  Q  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 243 ILKDRSSSLQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTA 302

Query: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMA 361
           MDLLCGCA+GRAALL HGGGMAVVSKKILRVSQ+GSERAVRIL+SVAK+SGSPAVL EMA
Sbjct: 303 MDLLCGCADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMA 362

Query: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           QLGIVAKLCLV+Q+E+GSKTKEKAKEILKMH+RVW+NS CIPSKLASSYP N
Sbjct: 363 QLGIVAKLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of CmaCh06G005270 vs. ExPASy TrEMBL
Match: A0A1S3B6U7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486683 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.4e-160
Identity = 308/412 (74.76%), Postives = 357/412 (86.65%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIP-ADSD 61
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCPITKLP+  +DSD
Sbjct: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSDSD 62

Query: 62  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121
            LTPNHTLRRLIQAWCTL+S +G+ERFPTPKPPI+K QI  +IS+S+++PSSL+S IRRL
Sbjct: 63  LLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIRRL 122

Query: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 181
           RS++AESE+NRRC+E AGAPEFL SVI++S S         + HEALSTLHNLRLSDS  
Sbjct: 123 RSISAESETNRRCVEFAGAPEFLVSVIVDSDSS--------ASHEALSTLHNLRLSDSTF 182

Query: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLLKSLLEVADPMELTFLKSELFVEIVG 241
           KSL  + E+ +SLT  M  +QGT+E SR YA L+LKS++EVADP++L+FLK ELFV+IV 
Sbjct: 183 KSLATHPEVLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVE 242

Query: 242 ILKDQTSQ-QSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTA 301
           ILKD++S  Q  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 243 ILKDRSSSLQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILLSSPEKRVCEMTLTA 302

Query: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMA 361
           MDLLCGCA+GRAALL HGGGMAVVSKKILRVSQ+GSERAVRIL+SVAK+SGSPAVL EMA
Sbjct: 303 MDLLCGCADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMA 362

Query: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           QLGIVAKLCLV+Q+E+GSKTKEKAKEILKMH+RVW+NS CIPSKLASSYP N
Sbjct: 363 QLGIVAKLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of CmaCh06G005270 vs. ExPASy TrEMBL
Match: A0A0A0LB33 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G782630 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.5e-159
Identity = 305/412 (74.03%), Postives = 353/412 (85.68%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPI-PADSD 61
           E+IEVP+YFLCPISLQIMKDPVT+PSGITYDR SIETWLFS KN+SCP+TKLP+  +DSD
Sbjct: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSD 62

Query: 62  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 121
            LTPNHTLRRLIQAWCTL+S HG+ERFPTPKPPI+K QI  +IS+S+++PSS +S IRRL
Sbjct: 63  LLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHIISTSNTSPSSQISSIRRL 122

Query: 122 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 181
           RS++AESE+NRRC+E AGAPEFL SVI+ S S         + HEALSTLHNLRLSDS  
Sbjct: 123 RSISAESETNRRCVEFAGAPEFLVSVIVGSDSS--------ASHEALSTLHNLRLSDSTF 182

Query: 182 KSLTVNAELFDSLTKVM--EQGTYE-SRAYAALLLKSLLEVADPMELTFLKSELFVEIVG 241
           KSL    E  +SLT  M  +QGT+E SR YA L+LKS++EVA+P++L+FLK ELFV+IV 
Sbjct: 183 KSLATRPEFLESLTDFMKLQQGTHESSRTYAVLILKSIIEVAEPIQLSFLKPELFVQIVE 242

Query: 242 ILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTA 301
           ILKD+ +SQQ  KAALGILI     GRN++KAVEAGGV ALVEILLS PE+RV EM LTA
Sbjct: 243 ILKDRSSSQQIFKAALGILIAVSPLGRNRLKAVEAGGVRALVEILLSSPEKRVCEMTLTA 302

Query: 302 MDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMA 361
           MD+LCGCA+GRAALL HGGGMAVVSKKILRVSQ+GSERAVRIL+SVAK+SGSPAVL EMA
Sbjct: 303 MDILCGCADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMA 362

Query: 362 QLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           QLGIVAKLCLVLQ+E+G KTKEKAKEILKMH+R+W+NS CIPSKLASSYP N
Sbjct: 363 QLGIVAKLCLVLQIENGGKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 406

BLAST of CmaCh06G005270 vs. NCBI nr
Match: XP_023005635.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 780.8 bits (2015), Expect = 5.8e-222
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. NCBI nr
Match: XP_022952378.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 775.0 bits (2000), Expect = 3.2e-220
Identity = 405/408 (99.26%), Postives = 405/408 (99.26%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFS KNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSNKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETA ISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALL KSLLEVADPMELTFLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLFKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. NCBI nr
Match: KAG7028156.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 774.2 bits (1998), Expect = 5.4e-220
Identity = 405/408 (99.26%), Postives = 405/408 (99.26%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETA ISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETAPISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMEL FLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELAFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSG PAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGCPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. NCBI nr
Match: KAG6596619.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 773.1 bits (1995), Expect = 1.2e-219
Identity = 404/408 (99.02%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETA ISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMEL FLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELAFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSG PAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGCPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           V KLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VTKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. NCBI nr
Match: XP_023540239.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 770.8 bits (1989), Expect = 6.0e-219
Identity = 404/408 (99.02%), Postives = 404/408 (99.02%), Query Frame = 0

Query: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60
           MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSDHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180
           RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETA ISCHEALSTLHNLRLSDSKL
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240
           KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMALTAMDLL 300
           DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEM LTAMDLL
Sbjct: 241 DQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMVLTAMDLL 300

Query: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGI 360
           CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILF VAKYSGSPAVLTEMAQLGI
Sbjct: 301 CGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFWVAKYSGSPAVLTEMAQLGI 360

Query: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 409
           VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN
Sbjct: 361 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of CmaCh06G005270 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 444.9 bits (1143), Expect = 7.0e-125
Identity = 236/435 (54.25%), Postives = 309/435 (71.03%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDS 61
           +EIE+P++FLCPISL IMKDPV V +GITYDR SIE WLFS K  SCP+TK  I      
Sbjct: 3   QEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVI--TETD 62

Query: 62  LTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLR 121
           LTPNHTLRRLIQ+WCTL++ +GIER PTPKPPI K +I KLI  SSS+  + + C++RLR
Sbjct: 63  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 122

Query: 122 SLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALIS------------------- 181
            + +E+ +N+RC+E+A  PEFLA+++ NS+   ++ ++ +S                   
Sbjct: 123 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 182

Query: 182 ----CHEALSTLHNLRLSDSKLKSLTVN---AELFDSLTKVMEQGTYESRAYAALLLKSL 241
                 EALS L++L  S++ LKSL  N     L  +LTK+M++G YESRAYAALLLK L
Sbjct: 183 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 242

Query: 242 LEVADPMELTFLKSELFVEIVGILKDQTSQQSLKAALGILITACTWGRNKVKAVEAGGVV 301
           LEVADPM++  L+ ELF E++ IL DQ S ++ ++A+ IL+  C WGRN+ KAVE G + 
Sbjct: 243 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 302

Query: 302 ALVEILLS---GPERRVSEMALTAMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGS 361
            ++E+L+      ERR SEMA+  +D+LC CAEGRA  L HG  +AVVSKKILRVSQ+ S
Sbjct: 303 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 362

Query: 362 ERAVRILFSVAKYSGSPAVLTEMAQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWR 408
           ERAVR+L SV ++  +P++L EM QLG+VAKLCLVLQV  G+KTKEKAKE+LK+HARVWR
Sbjct: 363 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWR 422

BLAST of CmaCh06G005270 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 441.0 bits (1133), Expect = 1.0e-123
Identity = 230/411 (55.96%), Postives = 307/411 (74.70%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDS 61
           EEIE+P +FLCPISL+IMKDPV V +GITYDR SIE WLF+ K  SCP+TK  I  D+D 
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI-TDAD- 67

Query: 62  LTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRLR 121
           LTPNHTLRRLIQ+WCTL++ +G+ER PTP+PPI K +I KLI  S+S+  + + C++RLR
Sbjct: 68  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 127

Query: 122 SLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKLK 181
            + +E+ +N+RC+E+AG PEFLA+++      ND+E   ++  EAL+ L++L  S++ LK
Sbjct: 128 QIVSENATNKRCLEAAGVPEFLANIV-----SNDSENGSLT-DEALNLLYHLETSETVLK 187

Query: 182 SLTVNAE---LFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGI 241
           +L  N +   +  SLTK+M++G YESR YA LLLK++LEVADPM+   LK E+F E+V I
Sbjct: 188 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQI 247

Query: 242 LKDQTSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLS---GPERRVSEMALT 301
           L D+ SQ++ KAA+ IL+  C WGRN+ KAVEAG +  ++E+L+      ERR  EMA+ 
Sbjct: 248 LDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMV 307

Query: 302 AMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEM 361
            +DLLC CAEGRA  L HG  +AVV KKILRVSQ  S+RAVR+L SV ++  +PA+L EM
Sbjct: 308 VLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEM 367

Query: 362 AQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYP 407
            QLG+VAKLCLVLQV  G KTKEKAKE+LK+HARVW++S C+P  +  +YP
Sbjct: 368 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of CmaCh06G005270 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 258.8 bits (660), Expect = 7.1e-69
Identity = 170/437 (38.90%), Postives = 241/437 (55.15%), Query Frame = 0

Query: 2   EEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDS 61
           EEIE+PNYF+CPISL+IMKDPVT  SGITYDR +I  WL   K  SCP+TK P+P DSD 
Sbjct: 20  EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD- 79

Query: 62  LTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLL---SCIR 121
           LTPNH LRRLIQ WC  +   G+ R  TP+ P  K  + + I +        L     ++
Sbjct: 80  LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQ 139

Query: 122 RLRSLAAESESNRRCIESAGAPEFLASVIINSIS-DNDNETALISCHEALSTLHNLRLSD 181
           +L  LA +  +NRR +   G  + L   ++   S D D    +    E+L  LH + +  
Sbjct: 140 KLEVLAMDG-NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 199

Query: 182 SKLKSLTV-NAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIV 241
           +  K++ + N  + +SLT V+ Q  + S+AY  +LL++L E      +  L  E+F  I+
Sbjct: 200 NDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGII 259

Query: 242 GILKD----------------QTS-------------------QQSLKAALGILITACTW 301
           G LKD                Q+S                   +Q++ AAL IL+   +W
Sbjct: 260 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 319

Query: 302 GRNKVKAVEAGGVVALVEILLS-GPERRVSEMALTAMDLLCGCAEGRAALLGHGGGMAVV 361
            RN+   V+ G V  L+E+ +S   E+R++E+ L  +  LC CA GRA +L H GG+AVV
Sbjct: 320 SRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVV 379

Query: 362 SKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQLGIVAKLCLVLQVESGSKTKEKA 398
           +K++LRVS    +RA+ IL +V+K+S    V+ EM  +G V KLC VL ++ G   KEKA
Sbjct: 380 TKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNLKEKA 439

BLAST of CmaCh06G005270 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 189.9 bits (481), Expect = 4.1e-48
Identity = 129/406 (31.77%), Postives = 221/406 (54.43%), Query Frame = 0

Query: 4   IEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDSLT 63
           I++P +F CPISL +M DPVT+ +G TYDR SI++W+ +  N +CP+T++ +     +L 
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVAL--SDFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLS------CI 123
           PNHTLRRLIQ WC  +  +G+ER PTPK P +   +  L+S +S+   + +S       I
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAI 131

Query: 124 RRLRSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSD 183
           RRLR LA +SE NR  I    A E L  ++   I      + L+S  E+L+ L  L +++
Sbjct: 132 RRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVS--ESLALLVLLHMTE 191

Query: 184 SKLKSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKS---ELFVE 243
           ++ +++  +      +T+++   + E R  AA L++ +L  A  M+L  + S    +F  
Sbjct: 192 TECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 244 IVGILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEMA 303
           ++ +LK+  +S+++LK  +  +   C   + +  A+ AG    L++ L +  +R  +E  
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTERG 311

Query: 304 LTAMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLT 363
           L  ++LLC   EG AA   H   + ++ K ILRVS   +E A   L ++   +       
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEERCRD 371

Query: 364 EMAQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPS 400
           E A  G+V +L L++Q +   + K KA+ +LK+    W + + + S
Sbjct: 372 EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of CmaCh06G005270 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 182.2 bits (461), Expect = 8.5e-46
Identity = 128/399 (32.08%), Postives = 221/399 (55.39%), Query Frame = 0

Query: 4   IEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSDSLT 63
           I++P +F CPISL++M+DPVTV +G TYDR SIE+W+    N +CP+T+ P+     +L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL--SDFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLS------CI 123
           PNHTLRRLIQ WC  +  +G+ER PTPK P +   +  L+S +S+   + +S       +
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAAL 131

Query: 124 RRLRSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSD 183
           RRLR  A +S+ NR  I +  A E L  ++ +  + ++    L+S  E+L+ L  L +++
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSE----LVS--ESLALLVMLPITE 191

Query: 184 -SKLKSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSL---LEVADPMELTFLKSELFV 243
            ++  S++ +    + LT+++   + E+R  AA L++ +    + AD          +F 
Sbjct: 192 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFE 251

Query: 244 EIVGILKDQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILLSGPERRVSEM 303
            ++ +L++  +S+++LK  +  L   C+    +  A+ AG    L++ L +  +R  +E 
Sbjct: 252 GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTER 311

Query: 304 ALTAMDLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVL 363
           AL  ++LLC   EG AA   H   + ++ K ILRVS   +E A   L ++   +      
Sbjct: 312 ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWR 371

Query: 364 TEMAQLGIVAKLCLVLQVESGSKTKEKAKEILKMHARVW 392
            E A  G+V +L L++Q E   + K+KA+++LK+    W
Sbjct: 372 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400