CmaCh06G005110 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G005110 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.2e-47
Identity = 146/503 (29.03%), Postives = 224/503 (44.53%), Query Frame = 0

Query: 8   CFSYILLLFSVSAIVDVNANSITLPLSAFPHHS------SSDPLQNLNFLASASQNRAHQ 67
           C  + L    +      + +S++ PL     HS      SS PL  L   +S+S++ A  
Sbjct: 4   CLIFFLYTTILQYYFHFSVSSLSTPLLLHLSHSLSTSKHSSSPLHLLK--SSSSRSSARF 63

Query: 68  IKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCS 127
            +        + SL   S   Y   LS G+    + L  DTGS LVWFPC   + C  C 
Sbjct: 64  RRHHHKQQQQQLSLPISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTCILCE 123

Query: 128 FPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQ---CRNCNQ---KTENCTQ 187
              + P         LSSS+  V C +P C         S      NC     +T +C  
Sbjct: 124 SKPLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNT 183

Query: 188 T---CPAYVVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSES 247
           +   CP +   YG GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  S
Sbjct: 184 SSYPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLS 243

Query: 248 LPSQMGL------KKFAYCLASRKFDDS--PHSGELIL--------------------DS 307
           LP+Q+ +        F+YCL S  FD         LIL                    D 
Sbjct: 244 LPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDD 303

Query: 308 GGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSI 367
             +K  +  +T   +NP    H Y  +Y +S++ I +G +++  P        +G GG +
Sbjct: 304 EKKKKNEFVFTEMLENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVV 363

Query: 368 IDSGSTFTFMDKPVFEAVAEAFEKQLAN-RTRATDVESATGLRPCFDISKEKSVEFPELI 427
           +DSG+TFT +    + +V E F+ ++     RA  VE ++G+ PC+ ++  ++V+ P L+
Sbjct: 364 VDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALV 423

Query: 428 FQFKGG-AKWTLALNNYFALVSSSG--------VACLTVVTHKEAAGGGSGPSVILGAFQ 458
             F G  +  TL   NYF      G        + CL ++   + +    G   ILG +Q
Sbjct: 424 LHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQ 483

BLAST of CmaCh06G005110 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.9e-36
Identity = 120/403 (29.78%), Postives = 178/403 (44.17%), Query Frame = 0

Query: 65  PKSNSVSKSSLSPHSYGA--YSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSF 124
           P+    S S +S  S G+  Y   L  GTP + ++++ DTGS +VW  C     C +C +
Sbjct: 122 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC-Y 181

Query: 125 PKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAYVV 184
            + DP+    F P+ S +   + C +P C        +     CN + + C      Y V
Sbjct: 182 SQSDPI----FDPRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTC-----LYQV 241

Query: 185 QYGSGS-TAGLLLSETLDFPDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMG 244
            YG GS T G   +ETL F   ++    +GC   +       +G+ G G+G  S P Q G
Sbjct: 242 SYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTG 301

Query: 245 LK---KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLS 304
            +   KF+YCL  R     P S   ++      +    +TP   NP +       +YY+ 
Sbjct: 302 HRFNQKFSYCLVDRSASSKPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYYVG 361

Query: 305 IRKILVGNQDVK-VPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRT 364
           +  I VG   V  V          G+GG IIDSG++ T + +P + A+ +AF        
Sbjct: 362 LLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLK 421

Query: 365 RATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVV 424
           RA D         CFD+S    V+ P ++  F+ GA  +L   NY   V ++G  C    
Sbjct: 422 RAPDFSL---FDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCF--- 481

Query: 425 THKEAAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
               A  G  G   I+G  QQQ F V YDL + R+GF    C+
Sbjct: 482 ----AFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CmaCh06G005110 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.3e-35
Identity = 145/485 (29.90%), Postives = 209/485 (43.09%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDP---------------- 60
           MA+P    +S +L L  VSAIV   A + +       HH    P                
Sbjct: 1   MASP---LYSVVLGLAIVSAIV---APTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKN 60

Query: 61  LQNLNFLASASQNRAHQIKTPKSNSVSKSSLSPHSY---GAYSAPLSFGTPPQTLHLIFD 120
           L     +  A +    ++++  +   S S +    Y   G Y   ++ GTP  +   I D
Sbjct: 61  LTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMD 120

Query: 121 TGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKS 180
           TGS L+W  C     C+QC F +  P+    F P+ SSS   + C++  C          
Sbjct: 121 TGSDLIWTQCEP---CTQC-FSQPTPI----FNPQDSSSFSTLPCESQYCQ--------- 180

Query: 181 QCRNCNQKTENCTQTCPAYVVQYGSGSTA-GLLLSETLDFPDQKIPNFVVGC----SFLS 240
                +  +E C      Y   YG GST  G + +ET  F    +PN   GC        
Sbjct: 181 -----DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFG 240

Query: 241 IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTP 300
               +G+ G G G  SLPSQ+G+ +F+YC+ S     SP +  L   + G   G  + T 
Sbjct: 241 QGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSPSTLALGSAASGVPEGSPSTTL 300

Query: 301 FRQ--NPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFM 360
                NP+        YYY++++ I VG  ++ +P        DG+GG IIDSG+T T++
Sbjct: 301 IHSSLNPT--------YYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 360

Query: 361 DKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISKEKS-VEFPELIFQFKGGAKWT 420
            +  + AVA+AF  Q+      T  ES++GL  CF    + S V+ PE+  QF GG    
Sbjct: 361 PQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG---V 420

Query: 421 LALNNYFALVS-SSGVACLTVVTHKEAAGGGSGPSV-ILGAFQQQNFYVEYDLVNERLGF 457
           L L     L+S + GV CL       A G  S   + I G  QQQ   V YDL N  + F
Sbjct: 421 LNLGEQNILISPAEGVICL-------AMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSF 435

BLAST of CmaCh06G005110 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 9.0e-33
Identity = 123/429 (28.67%), Postives = 181/429 (42.19%), Query Frame = 0

Query: 38  HHSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSSLSPHSY---GAYSAPLSFGTPPQ 97
           H  S   L     L  A +  + +++  ++     S +    Y   G Y   LS GTP Q
Sbjct: 47  HVDSGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQ 106

Query: 98  TLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWV 157
               I DTGS L+W  C     C+QC F +  P+    F P+ SSS   + C +  C  +
Sbjct: 107 PFSAIMDTGSDLIWTQCQP---CTQC-FNQSTPI----FNPQGSSSFSTLPCSSQLCQAL 166

Query: 158 FGPDVKSQCRNCNQKTENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPDQKIPNFVVGC- 217
             P               C+     Y   YG GS T G + +ETL F    IPN   GC 
Sbjct: 167 SSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCG 226

Query: 218 ---SFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELILDSGGEKT 277
                      +G+ G GRG  SLPSQ+ + KF+YC+       S  S  L+       T
Sbjct: 227 ENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSSTPSNLLLGSLANSVT 286

Query: 278 GDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKV-PYKYLVPGSDGSGGSIIDSG 337
                T   Q+  +       +YY+++  + VG+  + + P  + +  ++G+GG IIDSG
Sbjct: 287 AGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSG 346

Query: 338 STFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISKEKS-VEFPELIFQFK 397
           +T T+     +++V + F  Q+          S++G   CF    + S ++ P  +  F 
Sbjct: 347 TTLTYFVNNAYQSVRQEFISQI---NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD 406

Query: 398 GGAKWTLALNNYFALVSSSGVACLTVVTHKEAAGGGSGPSVILGAFQQQNFYVEYDLVNE 457
           GG    L   NYF +  S+G+ CL       A G  S    I G  QQQN  V YD  N 
Sbjct: 407 GG-DLELPSENYF-ISPSNGLICL-------AMGSSSQGMSIFGNIQQQNMLVVYDTGNS 434

BLAST of CmaCh06G005110 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.6e-29
Identity = 123/458 (26.86%), Postives = 197/458 (43.01%), Query Frame = 0

Query: 13  LLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSK 72
           LLL  ++A V   A  +++  +   H SS  PL+++  +A A  + A  +      + + 
Sbjct: 9   LLLLLLAATVAAAAAELSVYHNV--HPSSPSPLESI--IALARDDDARLLFLSSKAATAG 68

Query: 73  SSLSPHSYG----AYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPL 132
            S +P + G    +Y      G+P Q L L  DT +   W  C+    C  C    +   
Sbjct: 69  VSSAPVASGQAPPSYVVRAGLGSPSQQLLLALDTSADATWAHCSP---CGTCPSSSL--- 128

Query: 133 RIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAYVVQYGSGS 192
               F P  SSS   + C +  C    G    +     +      T    A+   +   S
Sbjct: 129 ----FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADAS 188

Query: 193 TAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPS------GIAGFGRGSESLPSQMGL--- 252
               L S+TL      IPN+  GC   S+  P+      G+ G GRG  +L SQ G    
Sbjct: 189 FQAALASDTLRLGKDAIPNYTFGC-VSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYN 248

Query: 253 KKFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKI 312
             F+YCL S  +     SG L L +GG +   + YTP  +NP  S+      YY+++  +
Sbjct: 249 GVFSYCLPS--YRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSS-----LYYVNVTGL 308

Query: 313 LVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDV 372
            VG+  VKVP       +    G+++DSG+  T    PV+ A+ E F +Q+A  +  T  
Sbjct: 309 SVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYT-- 368

Query: 373 ESATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEA 432
            S      CF+  +  +   P +     GG    L + N     S++ +ACL +    EA
Sbjct: 369 -SLGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLACLAMA---EA 428

Query: 433 AGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
               +    ++   QQQN  V +D+ N R+GF +++C+
Sbjct: 429 PQNVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

BLAST of CmaCh06G005110 vs. ExPASy TrEMBL
Match: A0A6J1KTP0 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111498572 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 7.1e-267
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 457

BLAST of CmaCh06G005110 vs. ExPASy TrEMBL
Match: A0A6J1FJU4 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111446087 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.4e-259
Identity = 446/457 (97.59%), Postives = 447/457 (97.81%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD NANSITLPLSA PH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHKEAA
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

BLAST of CmaCh06G005110 vs. ExPASy TrEMBL
Match: A0A6J1F3G5 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111441834 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.7e-231
Identity = 401/459 (87.36%), Postives = 419/459 (91.29%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MA PPPLCF YILLL SVSAI D   N ITLPLS+FPHHSSSDPLQ LNFLASASQNRAH
Sbjct: 1   MAPPPPLCFFYILLLSSVSAIAD--TNPITLPLSSFPHHSSSDPLQTLNFLASASQNRAH 60

Query: 61  QIKTP--KSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCS 120
           QIK P  KSNSVSKS LSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVW PCTSKYLCS
Sbjct: 61  QIKAPKSKSNSVSKSPLSPHSYGAYSTPLSFGTPSQTLHLIFDTGSSLVWLPCTSKYLCS 120

Query: 121 QCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCP 180
           +CSFPKIDP RIPRF+PKLSSSSKLVGCQNPKC W+FGPDVKSQCR+CN KTENCTQTCP
Sbjct: 121 ECSFPKIDPARIPRFIPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCP 180

Query: 181 AYVVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
           AYVVQYGSGSTAGLLLSETLDFP++KI NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG
Sbjct: 181 AYVVQYGSGSTAGLLLSETLDFPNKKITNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240

Query: 241 LKKFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRK 300
           LKKFAYCLASRKFDDSPH+GELILDS G KT  LTYTPFRQNPSVSNHAYKEYYYL+IRK
Sbjct: 241 LKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLTYTPFRQNPSVSNHAYKEYYYLTIRK 300

Query: 301 ILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATD 360
           I VGN+ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPVFEAVA+  EKQLANRTRATD
Sbjct: 301 IFVGNKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATD 360

Query: 361 VESATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKE 420
           VES TGLRPCFDISK+KSVEFPEL F  KGGAKW   L+NYFALVSSSGVACLTVVTHK 
Sbjct: 361 VESLTGLRPCFDISKDKSVEFPELTFHLKGGAKWAPPLSNYFALVSSSGVACLTVVTHKA 420

Query: 421 AAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           A  GG GPS+ILGAFQQQNFYVEYDLVN+++GFRQQTCS
Sbjct: 421 AESGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of CmaCh06G005110 vs. ExPASy TrEMBL
Match: A0A6J1IXY3 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111481640 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.4e-230
Identity = 398/459 (86.71%), Postives = 419/459 (91.29%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MA PPPLCF YILL+ SVSAI D   N IT+PLS+FPHHSSSDPLQ LNFLASASQNRAH
Sbjct: 1   MAPPPPLCFFYILLVSSVSAIAD--TNPITIPLSSFPHHSSSDPLQTLNFLASASQNRAH 60

Query: 61  QIKTPK--SNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCS 120
           QIK PK  SNSVSKS LSPHSYGAYS PLSFGTPPQTLHLIFDTGSSLVW PCTSKYLCS
Sbjct: 61  QIKAPKSESNSVSKSPLSPHSYGAYSTPLSFGTPPQTLHLIFDTGSSLVWLPCTSKYLCS 120

Query: 121 QCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCP 180
           +CSFPKIDP  IPRF+PKLSS+SKLVGCQNPKC W+FGPDVKSQCR+CN KTENCTQTCP
Sbjct: 121 ECSFPKIDPAGIPRFIPKLSSTSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCP 180

Query: 181 AYVVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240
           AYVVQYGSGSTAGLLLSETLDFPD+K  NFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG
Sbjct: 181 AYVVQYGSGSTAGLLLSETLDFPDKKFTNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMG 240

Query: 241 LKKFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRK 300
           LKKFAYCLASRKFDDSPH+GELILDS G KT  L+YTPFRQNPSVSNHAYKEYYYL+IRK
Sbjct: 241 LKKFAYCLASRKFDDSPHAGELILDSSGAKTSGLSYTPFRQNPSVSNHAYKEYYYLTIRK 300

Query: 301 ILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATD 360
           I VG + VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPVFEAVA+  EKQLANRTRATD
Sbjct: 301 IFVGKKAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVFEAVAQEIEKQLANRTRATD 360

Query: 361 VESATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKE 420
           VES TGLRPCFDISK+KSVEFPEL FQ KGGAKW L L+NYFALVSSSGVACLTVVTHK 
Sbjct: 361 VESLTGLRPCFDISKDKSVEFPELTFQLKGGAKWGLPLSNYFALVSSSGVACLTVVTHKT 420

Query: 421 AAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           A  GG GPS+ILGAFQQQNFYVEYDLVN+++GFRQQTCS
Sbjct: 421 ADSGG-GPSIILGAFQQQNFYVEYDLVNQKIGFRQQTCS 456

BLAST of CmaCh06G005110 vs. ExPASy TrEMBL
Match: A0A5A7TRK2 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004410 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.2e-228
Identity = 394/457 (86.21%), Postives = 418/457 (91.47%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MA+P PL F YILL  S+SAI   N+N ITLPL++ PH SSSDPLQ L FLASAS+NRAH
Sbjct: 1   MASPSPLSFFYILLFSSLSAI--SNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           +IKTPKSNSVSKS LSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVWFPCTS+YLC++C
Sbjct: 61  RIKTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDP  IPRFVPKLSSSSKLVGCQNPKC W+FGPDVKSQCR+CN KTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFP++KIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDS HSG+LILDS G KT  LTYT FRQNPSVSNHAYKEYYYL+IRKI+
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPV + VA+ FEKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           + TGLRPCFD+SKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTH    
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGG GPSVILGAFQQQNFYVEYDLVNERLGFR+QTC+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

BLAST of CmaCh06G005110 vs. NCBI nr
Match: XP_023005632.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 929.1 bits (2400), Expect = 1.5e-266
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 457

BLAST of CmaCh06G005110 vs. NCBI nr
Match: KAG7028143.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-259
Identity = 446/457 (97.59%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD NANSITLPLSAFPH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHKEA+
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

BLAST of CmaCh06G005110 vs. NCBI nr
Match: XP_022940517.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 904.8 bits (2337), Expect = 3.0e-259
Identity = 446/457 (97.59%), Postives = 447/457 (97.81%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD NANSITLPLSA PH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHKEAA
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

BLAST of CmaCh06G005110 vs. NCBI nr
Match: XP_023540611.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.4 bits (2336), Expect = 3.9e-259
Identity = 446/457 (97.59%), Postives = 447/457 (97.81%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD N NSITLPLSAFPH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANTNSITLPLSAFPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNLKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
           VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGL PCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHK+AA
Sbjct: 361 SATGLLPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKDAA 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 457

BLAST of CmaCh06G005110 vs. NCBI nr
Match: KAG6596604.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902.1 bits (2330), Expect = 1.9e-258
Identity = 444/457 (97.16%), Postives = 446/457 (97.59%), Query Frame = 0

Query: 1   MAAPPPLCFSYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAH 60
           MAAPPPLCFSYILLLFSVSAIVD NANSITLPLSA PH SSSDPLQNLNFLASASQNRAH
Sbjct: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSAIPHPSSSDPLQNLNFLASASQNRAH 60

Query: 61  QIKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSVSKS LSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC
Sbjct: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAY 180
           SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKC WVFGPDVKSQCRNCN KTENCTQTCPAY
Sbjct: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180

Query: 181 VVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSGELILDSGG KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL
Sbjct: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQL NRTRATDVE
Sbjct: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLVNRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAA 420
           SATGLRPCFDISKEKSVEFPELIFQFKGGAKW L LNNYFALVSSSGVACLTVVTHKEA+
Sbjct: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAS 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
           GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQ+CS
Sbjct: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 457

BLAST of CmaCh06G005110 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 263/472 (55.72%), Postives = 334/472 (70.76%), Query Frame = 0

Query: 9   FSYILLLFSVSAIVDVNANSITLPLSAFPH--HSSSDPLQNLNFLASASQNRAHQIK--- 68
           F + L+  SV        +++ LPLS F H   S  DP  +L  LA +S  RAH++K   
Sbjct: 6   FFFFLIFLSV-------VSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGT 65

Query: 69  ------------TPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPC 128
                       T  S +V KS LS  SYG YS  LSFGTP QT+  +FDTGSSLVW PC
Sbjct: 66  SIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPC 125

Query: 129 TSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQCRNCNQKTE 188
           TS+YLCS C F  +DP  IPRF+PK SSSSK++GCQ+PKC +++GP+V  QCR C+  T 
Sbjct: 126 TSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTR 185

Query: 189 NCTQTCPAYVVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSE 248
           NCT  CP Y++QYG GSTAG+L++E LDFPD  +P+FVVGCS +S  QP+GIAGFGRG  
Sbjct: 186 NCTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPV 245

Query: 249 SLPSQMGLKKFAYCLASRKFDDSPHSGELILDSG-----GEKTGDLTYTPFRQNPSVSNH 308
           SLPSQM LK+F++CL SR+FDD+  + +L LD+G     G KT  LTYTPFR+NP+VSN 
Sbjct: 246 SLPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNK 305

Query: 309 AYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAF 368
           A+ EYYYL++R+I VG + VK+PYKYL PG++G GGSI+DSGSTFTFM++PVFE VAE F
Sbjct: 306 AFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEF 365

Query: 369 EKQLANRTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWTLALNNYFALVSSS 428
             Q++N TR  D+E  TGL PCF+IS +  V  PELIF+FKGGAK  L L+NYF  V ++
Sbjct: 366 ASQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNT 425

Query: 429 GVACLTVVTHKEA-AGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
              CLTVV+ K     GG+GP++ILG+FQQQN+ VEYDL N+R GF ++ CS
Sbjct: 426 DTVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

BLAST of CmaCh06G005110 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-48
Identity = 146/503 (29.03%), Postives = 224/503 (44.53%), Query Frame = 0

Query: 8   CFSYILLLFSVSAIVDVNANSITLPLSAFPHHS------SSDPLQNLNFLASASQNRAHQ 67
           C  + L    +      + +S++ PL     HS      SS PL  L   +S+S++ A  
Sbjct: 4   CLIFFLYTTILQYYFHFSVSSLSTPLLLHLSHSLSTSKHSSSPLHLLK--SSSSRSSARF 63

Query: 68  IKTPKSNSVSKSSLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCS 127
            +        + SL   S   Y   LS G+    + L  DTGS LVWFPC   + C  C 
Sbjct: 64  RRHHHKQQQQQLSLPISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPC-RPFTCILCE 123

Query: 128 FPKIDPLRIPRFVPKLSSSSKLVGCQNPKCDWVFGPDVKSQ---CRNCNQ---KTENCTQ 187
              + P         LSSS+  V C +P C         S      NC     +T +C  
Sbjct: 124 SKPLPPSP----PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNT 183

Query: 188 T---CPAYVVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSES 247
           +   CP +   YG GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  S
Sbjct: 184 SSYPCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLS 243

Query: 248 LPSQMGL------KKFAYCLASRKFDDS--PHSGELIL--------------------DS 307
           LP+Q+ +        F+YCL S  FD         LIL                    D 
Sbjct: 244 LPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDD 303

Query: 308 GGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSI 367
             +K  +  +T   +NP    H Y  +Y +S++ I +G +++  P        +G GG +
Sbjct: 304 EKKKKNEFVFTEMLENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVV 363

Query: 368 IDSGSTFTFMDKPVFEAVAEAFEKQLAN-RTRATDVESATGLRPCFDISKEKSVEFPELI 427
           +DSG+TFT +    + +V E F+ ++     RA  VE ++G+ PC+ ++  ++V+ P L+
Sbjct: 364 VDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALV 423

Query: 428 FQFKGG-AKWTLALNNYFALVSSSG--------VACLTVVTHKEAAGGGSGPSVILGAFQ 458
             F G  +  TL   NYF      G        + CL ++   + +    G   ILG +Q
Sbjct: 424 LHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQ 483

BLAST of CmaCh06G005110 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 185.7 bits (470), Expect = 8.6e-47
Identity = 144/486 (29.63%), Postives = 217/486 (44.65%), Query Frame = 0

Query: 10  SYILLLFSVSAIVDVNANSITLPLSAFPHHSSSDPLQNLNFLASASQNRAHQIKTPKSNS 69
           +++L LF +  ++    N         P  SSS      +FL       +  + TPKS +
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSS------SFLVLTLTKSSVSLPTPKSQT 64

Query: 70  VSKSSLSPHSYGAYSAP-----------LSFGTPPQTLHLIFDTGSSLVWFPCTS-KYLC 129
             +      S      P           L+ GTPPQ + +  DTGS L W PC +  + C
Sbjct: 65  QERIKKPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDC 124

Query: 130 SQCSFPKIDPLRIPR-FVPKLSSSSKLVGCQNPKC------DWVFGPDVKSQCRNCNQKT 189
            +C   K + L+ P  F P  SS+S    C +  C      D  F P   + C       
Sbjct: 125 IECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLK 184

Query: 190 ENCTQTCPAYVVQYGSGS-TAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRG 249
             C + CP++   YG G   +G+L  + L    + +P F  GC   +  +P GIAGFGRG
Sbjct: 185 STCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRG 244

Query: 250 SESLPSQMGL--KKFAYCLASRKFDDSPH-SGELILDSGG---EKTGDLTYTPFRQNPSV 309
             SLPSQ+G   K F++C    KF ++P+ S  LIL +       T  L +TP    P  
Sbjct: 245 LLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTP-- 304

Query: 310 SNHAYKEYYYLSIRKILVGNQ--DVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEA 369
               Y   YY+ +  I +G      +VP       S G+GG ++DSG+T+T + +P +  
Sbjct: 305 ---MYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQ 364

Query: 370 VAEAFEKQLANRTRATDVESATGLRPCFDI--------SKEKSVE--FPELIFQFKGGAK 429
           +    +  +    RAT+ ES TG   C+ +        S E  V   FP + F F   A 
Sbjct: 365 LLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNAT 424

Query: 430 WTLAL-NNYFALVSSSGVACLTVVTHKEAAGGGSGPSVILGAFQQQNFYVEYDLVNERLG 457
             L   N+++A+ + S  + +  +  +    G  GP+ + G+FQQQN  V YDL  ER+G
Sbjct: 425 LLLPQGNSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKERIG 478

BLAST of CmaCh06G005110 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-40
Identity = 126/398 (31.66%), Postives = 179/398 (44.97%), Query Frame = 0

Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   +  GTPP+   LI DTGS L W  C   Y C   +    D        PK S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYD--------PKTSAS 217

Query: 141 SKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
            K + C +P+C  +  PD   QC + N       Q+CP Y   YG  S T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 201 F---------PDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 260
                      + K+ N + GC   +       SG+ G GRG  S  SQ+       F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 261 CLASRKFDDSPHSGELILDSGGEKTGDLTYTPFRQNPSVS--NHAYKEYYYLSIRKILVG 320
           CL  R   ++  S +LI    GE    L +T       V+   ++ + +YY+ I+ ILVG
Sbjct: 338 CLVDRN-SNTNVSSKLIF---GEDKDLLNHTNLNFTSFVNGKENSVETFYYIQIKSILVG 397

Query: 321 NQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAF-EKQLANRTRATDVES 380
            + + +P +     SDG GG+IIDSG+T ++  +P +E +   F EK   N     D   
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457

Query: 381 ATGLRPCFDIS--KEKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEA 440
              L PCF++S  +E ++  PEL   F  G  W     N F  +S   + CL ++     
Sbjct: 458 ---LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSED-LVCLAIL----- 517

Query: 441 AGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQTCS 458
            G       I+G +QQQNF++ YD    RLGF    C+
Sbjct: 518 -GTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

BLAST of CmaCh06G005110 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.5e-40
Identity = 116/383 (30.29%), Postives = 172/383 (44.91%), Query Frame = 0

Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   +  G P + ++++ DTGS + W  CT    C+ C + + +P+    F P  SSS
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADC-YHQTEPI----FEPSSSSS 205

Query: 141 SKLVGCQNPKCDWVFGPDVKSQCRNCNQKTENCTQTCPAYVVQYGSGS-TAGLLLSETLD 200
            + + C  P+C+ +      S+CRN          TC  Y V YG GS T G   +ETL 
Sbjct: 206 YEPLSCDTPQCNAL----EVSECRNA---------TC-LYEVSYGDGSYTVGDFATETLT 265

Query: 201 FPDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPH 260
                + N  VGC   +       +G+ G G G  +LPSQ+    F+YCL  R  D +  
Sbjct: 266 IGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSAS- 325

Query: 261 SGELILDSGGEKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPG 320
                +D G   + D    P      + NH    +YYL +  I VG + +++P       
Sbjct: 326 ----TVDFGTSLSPDAVVAPL-----LRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMD 385

Query: 321 SDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLR---PCFDISK 380
             GSGG IIDSG+  T +   ++ ++ ++F K         D+E A G+     C+++S 
Sbjct: 386 ESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVK------GTLDLEKAAGVAMFDTCYNLSA 445

Query: 381 EKSVEFPELIFQFKGGAKWTLALNNYFALVSSSGVACLTVVTHKEAAGGGSGPSVILGAF 440
           + +VE P + F F GG    L   NY   V S G  CL       A    +    I+G  
Sbjct: 446 KTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCL-------AFAPTASSLAIIGNV 483

Query: 441 QQQNFYVEYDLVNERLGFRQQTC 457
           QQQ   V +DL N  +GF    C
Sbjct: 506 QQQGTRVTFDLANSLIGFSSNKC 483