CmaCh06G004620 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G004620
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	subtilisin-like protease SBT4.15
Location	Cma_Chr06: 2168960 .. 2171995 (-)
RNA-Seq Expression	CmaCh06G004620
Synteny	CmaCh06G004620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTACAAAATGCTCAAAGCGTTCCTCTCATTCATTTCCCTCTTCGCCGCCGTGGCCTCCTCAATCCCCGTTCCCAGAAAGGTATAAAAACCCCACCGCCGCTGCCACACGGTGGTTTCTGCCACCATTTTCTTAATTTAAAATATGAACTTGGATTTGGACAGGCTTATGTTGTTTATATGGGAGCATTGCCAAAGGTTCAGAGCTATGGAGTTCTTGAAGAATATCATCACAGCCTTCTTGCTAATGCAGTCGGAGAGTAATTAATTATTATTATTGCCCAAAAAATGAAATTTTTGTTGGGTTTTTTTTTTTTTTTTTTTTTGTGTGTTTATTTTGGGTAAATTTTTTATTTTTTTAATGGGTTTTTATTTATTTATTTTAGTGAGGAATTGGCGAGAAAATCCAAGATTTATAGCTATGGAAGGAGCTTCAATGGGTTTGCTGCAATGCTCTTACCCCATGAAGCCCAGAAACTAGCAAGTTCGTCTGAATTTCCTTAGCTTTACCTAAAATCTATAAATATTATCACAATATAATTTAGGAATAATTTCCTTTTCATTTTTTCAAAAAAAATTGAGTTTTTAAGATCTAATAATTGTTATATAGAACCCGATTCGTATAATATTGTTTGCATAAGTGTTCATGACTTTACTTTGAGTTTTCTCAAAAAAACCTTAGCTGATGTGAGACTTTTATCATCCAACACCTCTTTCCTCGACTCTTTTTTCTTTTGGAGTATTAGTCATTTTAGTTTAGAGCATGACTCTAATATCATATTAGATGAATACGACTCTCCACAATTGTACGATATTGTCCACTTTGAACATAAGAAGCCTCGTACTAATATGGAGATAGTATTCCTTGACTATAAATTCATGATCATTCTTTAAATTAGCTGAGGTCGGACTTTGATTATCCAATAATAATAATACAGAGGAAAAAGGCGTGGTGTCTGTATTTTGGAGCACTACAAGGAAGTTGCACACGACAAGATCATGGGATTTTTGGACAAGATCATAGGATTTTTTGGGGCTGTCAGTGGCTGCCAGCCGCCAGAACGCCGCCACAGAGTCGGACATAATCGTCGGATTGTTGGACTCCGGGATTTGGATGGAGAGTCCCAGTTTCAAGGACGATGGCTATGGCGAAATTCCTTCTAAGGGGAAAGGCCACTGTGTCACTGGCCATAACTTTACCGGCTGCAACAGGTTTCTCTTTCTTCTTCTTTTCTATTACTTTAAGGTTTTTTTTTTTTTTTTTTTAATATGTTTTTGTTTGTTTGAAATAATTATCTTATAATAAATTAATTATAAACTCATTAAAATAATGAGTATACTATGAACAACAAAGTTTATCAAGTCCAATTTTATTTAATTTCACAATTTTTTTTTGCATAAAATTAAGGTTGAAAATGAGGGTCTAAATTGTAATTTCGCACATTCAGGAAAGTGATCGGCGCCCGGTTCTTCAACCTGCAGGAAATAGACGCCACAATCAACCAAAGCCCCACGGACGAGGTGGGCCACGGCTCCCACACCTCCTCCACCGTCGCGGGAGCCACCGTAGACGGCGCCAGCCTCTACGGCATCGCCGGAGGCACCGCACGCGGCGGCGTCCCGGCAGCGAGAATCGCCATGTACAAAGTATGCTGGAACGTAGGGTGCACGGACGCGGACCTTCTGGCCGGATTCGACCACGCAATCGCCGACGGAGTGGACATAATATCGGTGTCGATCGGTGGCGGGTTGCGGGAATTCTTCGAGGATCCGATCGCAATTGGGTCGTTTCACGCAATGGCAAAAGGGATACTGACGAGCTGCTCCGCCGGAAACAACGGGCCGGGGTTGACAACGGTGGAGAATACGGCGCCGTGGATTATGACGGTGGCGGCTTCCACCATTGATAGAGATTTCAGTACCGTCATGAAGCTGGGAAACAACAAGAAATTATCTGTGAGTATAAAATTAATGAAAATAAAAGCATTTGAAACCCAAAATTAAGGCTTAATCCATGCCTTGAATGAAGCAGGGAATATCACTGAATACATTTTCAACCAAGAAGAAGATGTACCCTCTCATCAGCGGAGGAAGTGCAGCGTTACCCAATCATCCGAATGCAAGGTACGAATAACTAGTTTCCAATAAATCTACGAGAGATTATTTGTAAAATAGCTAAGTGATTCCCTTTTTAAATTGAGTTGCCCAGAAGATGTTGAAATACGAGATCAACGAATACATATGCTCCTAGAAGCATACAAGTACTATAACGTATCTCTACATGGGTATCTGGTATATGCTCCTAGAAGCGTACAAGTACTATAATGTACCTTTATGTGGGTATTTGGTAACCATTACTAGAACGTGCCTCTATGTGGGTATCTGGTAAACAGTATTGTAATGTGCCTCTATGTGGGTATCTTGTACCCGGTACTGGTAACTAGTACTGTAATGTGCTCCTCCATAGGTATCTGGTAACCAGTACTGCAATAGTATTTTTAGTGTTATGGGATATGATTGATTAGCGGTTCATTAGAATCATATAAATCAATACTCAGAACGGTATGCTTTTGTCAATTTTAAGCATAAACTCGGGTGATTTTATATCAATCTTGAAGGTTTTGTGAAGAAAAGTTCTGGTTTTAGGTGGTGTGATGGTGGGAGTCTTGATAAGGGTAAAGTGGAAGGGAAAATATTGTATTGTTTGGGATCTTTATCTCAAGAATACAGTCTCGGAGCTTGGTGGTGTCGGAGTTATAGCCAATCTTATGAACAATATGGAGGTTGTTACCGTCACTCCAATTCCTGCTACTCATCTTTCCTCTCAAGATTCTGACATTGTTGAAGCCTATATCAACTCCATTAAGTATGTTCTTCCTTAAATTACGGGTTTCTTTTTTGGGTCGGGTGAAATATTTGAACCTTTAATCTTTTGTTCGGCTTTAAGAGGAAGCCTAAGGCGGTCATAATGAAGACAACCACTACGAAGACAGAGGCTCCTTTCTTGGCCTATTTTTCTTCAAGAGGTCCTTAG

mRNA sequence

ATGAATTACAAAATGCTCAAAGCGTTCCTCTCATTCATTTCCCTCTTCGCCGCCGTGGCCTCCTCAATCCCCGTTCCCAGAAAGGCTTATGTTGTTTATATGGGAGCATTGCCAAAGGTTCAGAGCTATGGAGTTCTTGAAGAATATCATCACAGCCTTCTTGCTAATGCAGTCGGAGATGAGGAATTGGCGAGAAAATCCAAGATTTATAGCTATGGAAGGAGCTTCAATGGGTTTGCTGCAATGCTCTTACCCCATGAAGCCCAGAAACTAGCAATGGCTGCCAGCCGCCAGAACGCCGCCACAGAGTCGGACATAATCGTCGGATTGTTGGACTCCGGGATTTGGATGGAGAGTCCCAGTTTCAAGGACGATGGCTATGGCGAAATTCCTTCTAAGGGGAAAGGCCACTGTGTCACTGGCCATAACTTTACCGGCTGCAACAGGAAAGTGATCGGCGCCCGGTTCTTCAACCTGCAGGAAATAGACGCCACAATCAACCAAAGCCCCACGGACGAGGTGGGCCACGGCTCCCACACCTCCTCCACCGTCGCGGGAGCCACCGTAGACGGCGCCAGCCTCTACGGCATCGCCGGAGGCACCGCACGCGGCGGCGTCCCGGCAGCGAGAATCGCCATGTACAAAGTATGCTGGAACGTAGGGTGCACGGACGCGGACCTTCTGGCCGGATTCGACCACGCAATCGCCGACGGAGTGGACATAATATCGGTGTCGATCGGTGGCGGGTTGCGGGAATTCTTCGAGGATCCGATCGCAATTGGGTCGTTTCACGCAATGGCAAAAGGGATACTGACGAGCTGCTCCGCCGGAAACAACGGGCCGGGGTTGACAACGGTGGAGAATACGGCGCCGTGGATTATGACGGTGGCGGCTTCCACCATTGATAGAGATTTCAGTACCGTCATGAAGCTGGGAAACAACAAGAAATTATCTGGAATATCACTGAATACATTTTCAACCAAGAAGAAGATGTACCCTCTCATCAGCGGAGGAAGTGCAGCGTTACCCAATCATCCGAATGCAAGGTGGTGTGATGGTGGGAGTCTTGATAAGGTCTCGGAGCTTGGTGGTGTCGGAGTTATAGCCAATCTTATGAACAATATGGAGGTTGTTACCGTCACTCCAATTCCTGCTACTCATCTTTCCTCTCAAGATTCTGACATTGTTGAAGCCTATATCAACTCCATTAAGAAGCCTAAGGCGGTCATAATGAAGACAACCACTACGAAGACAGAGGCTCCTTTCTTGGCCTATTTTTCTTCAAGAGGTCCTTAG

Coding sequence (CDS)

ATGAATTACAAAATGCTCAAAGCGTTCCTCTCATTCATTTCCCTCTTCGCCGCCGTGGCCTCCTCAATCCCCGTTCCCAGAAAGGCTTATGTTGTTTATATGGGAGCATTGCCAAAGGTTCAGAGCTATGGAGTTCTTGAAGAATATCATCACAGCCTTCTTGCTAATGCAGTCGGAGATGAGGAATTGGCGAGAAAATCCAAGATTTATAGCTATGGAAGGAGCTTCAATGGGTTTGCTGCAATGCTCTTACCCCATGAAGCCCAGAAACTAGCAATGGCTGCCAGCCGCCAGAACGCCGCCACAGAGTCGGACATAATCGTCGGATTGTTGGACTCCGGGATTTGGATGGAGAGTCCCAGTTTCAAGGACGATGGCTATGGCGAAATTCCTTCTAAGGGGAAAGGCCACTGTGTCACTGGCCATAACTTTACCGGCTGCAACAGGAAAGTGATCGGCGCCCGGTTCTTCAACCTGCAGGAAATAGACGCCACAATCAACCAAAGCCCCACGGACGAGGTGGGCCACGGCTCCCACACCTCCTCCACCGTCGCGGGAGCCACCGTAGACGGCGCCAGCCTCTACGGCATCGCCGGAGGCACCGCACGCGGCGGCGTCCCGGCAGCGAGAATCGCCATGTACAAAGTATGCTGGAACGTAGGGTGCACGGACGCGGACCTTCTGGCCGGATTCGACCACGCAATCGCCGACGGAGTGGACATAATATCGGTGTCGATCGGTGGCGGGTTGCGGGAATTCTTCGAGGATCCGATCGCAATTGGGTCGTTTCACGCAATGGCAAAAGGGATACTGACGAGCTGCTCCGCCGGAAACAACGGGCCGGGGTTGACAACGGTGGAGAATACGGCGCCGTGGATTATGACGGTGGCGGCTTCCACCATTGATAGAGATTTCAGTACCGTCATGAAGCTGGGAAACAACAAGAAATTATCTGGAATATCACTGAATACATTTTCAACCAAGAAGAAGATGTACCCTCTCATCAGCGGAGGAAGTGCAGCGTTACCCAATCATCCGAATGCAAGGTGGTGTGATGGTGGGAGTCTTGATAAGGTCTCGGAGCTTGGTGGTGTCGGAGTTATAGCCAATCTTATGAACAATATGGAGGTTGTTACCGTCACTCCAATTCCTGCTACTCATCTTTCCTCTCAAGATTCTGACATTGTTGAAGCCTATATCAACTCCATTAAGAAGCCTAAGGCGGTCATAATGAAGACAACCACTACGAAGACAGAGGCTCCTTTCTTGGCCTATTTTTCTTCAAGAGGTCCTTAG

Protein sequence

MNYKMLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSYGRSFNGFAAMLLPHEAQKLAMAASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSAALPNHPNARWCDGGSLDKVSELGGVGVIANLMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRGP

Homology

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BLAST of CmaCh06G004620 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 1.7e-113
Identity = 232/467 (49.68%), Postives = 291/467 (62.31%), Query Frame = 0


Query: 25  VPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSYGRSFNGFAAMLL 84
           V RK Y+VYMG   +  S     E HH+LL   +GDE  AR+ KIYSYG++ NGF A L 
Sbjct: 30  VERKPYIVYMGEATE-NSLVEAAENHHNLLMTVIGDESKARELKIYSYGKNINGFVARLF 89

Query: 85  PHEAQKLAM----------------------------AASRQNAATESDIIVGLLDSGIW 144
           PHEA+KL+                             +  +++   ES+IIVG+LD+GI 
Sbjct: 90  PHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGID 149

Query: 145 MESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQEIDATINQSPT--DEV 204
           +ESPSF D G G  P+K KG CVTG+NFT CN KVIGA++F++Q       +  T  D  
Sbjct: 150 VESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGDTAADHD 209

Query: 205 GHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNVGCTDADLLAGFDHA 264
           GHG+HTSST+AG +V  ASL+GIA GTARGGVP+ARIA YKVCW+ GCTD D+LA FD A
Sbjct: 210 GHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEA 269

Query: 265 IADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGNNGPGLTTVENTAPWIM 324
           I+DGVDIIS+SIGG    FFEDPIAIG+FHAM +GILT+CSAGNNGPGL TV N APW+M
Sbjct: 270 ISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVM 329

Query: 325 TVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSA---ALPNHPNARWC 384
           TVAA+++DR F TV+KLGN    SGISLN F+ +KKMYPL SG  A   +   +     C
Sbjct: 330 TVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPSTC 389

Query: 385 DGGSL--DK-------------------------VSELGGVGVIANLMNNMEVVTVTPIP 432
           + G+L  DK                         V  L G GVI  L+   ++ T T I 
Sbjct: 390 EPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIA 449

[Close]

BLAST of CmaCh06G004620 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 201/453 (44.37%), Postives = 263/453 (58.06%), Query Frame = 0


Query: 30  YVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSYGRSFNGFAAMLLPHEAQ 89
           Y++Y+G  P      +  + H +LL++    +E A++ K+YSY ++FN FAA L PHEA+
Sbjct: 38  YIIYLGDRPDNTEETI--KTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAK 97

Query: 90  KL-----AMAASR----------------------QNAATESDIIVGLLDSGIWMESPSF 149
           K+      ++ SR                      ++   E D+I+G+LD+GI  +S SF
Sbjct: 98  KMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESF 157

Query: 150 KDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQ-EIDATINQSPTDEVGHGSHTS 209
            D G G  P+K KG C    NFTGCN K+IGA++F     + A   +SP D  GHG+HTS
Sbjct: 158 LDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTS 217

Query: 210 STVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCW-NVGCTDADLLAGFDHAIADGVD 269
           STVAG  V  ASLYGIA GTARG VP+AR+AMYKVCW   GC D D+LAGF+ AI DGV+
Sbjct: 218 STVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVE 277

Query: 270 IISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGNNGPGLTTVENTAPWIMTVAAST 329
           IIS+SIGG + ++  D I++GSFHAM KGILT  SAGN+GP   TV N  PWI+TVAAS 
Sbjct: 278 IISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASG 337

Query: 330 IDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSAALPNHPN--ARWCDGGSLDK 389
           IDR F + + LGN K  SG+ ++ FS K K YPL+SG  AA        AR+C   SLD+
Sbjct: 338 IDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDR 397

Query: 390 --------------------VSELGGVGVIANLMNNMEVVTVTPIPATHLSSQDSDIVEA 432
                               +   GG G I      ++   +   PAT ++S   DI+  
Sbjct: 398 KKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYR 457

[Close]

BLAST of CmaCh06G004620 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.6e-82
Identity = 194/482 (40.25%), Postives = 264/482 (54.77%), Query Frame = 0


Query: 3   YKMLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEE 62
           +K+    L F +  A+   S    +  Y+VYMG   K++       +H ++L   VG   
Sbjct: 7   FKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGR--KLEDPDSAHLHHRAMLEQVVG-ST 66

Query: 63  LARKSKIYSYGRSFNGFAAMLLPHEAQKLAMAAS-------------------------- 122
            A +S +++Y RSFNGFA  L   EA+K+A                              
Sbjct: 67  FAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLT 126

Query: 123 -RQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGAR 182
             + +  ES+I+VG+LD+GIW ESPSF D+G+   P K KG C T +NF  CNRK+IGAR
Sbjct: 127 VPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRKIIGAR 186

Query: 183 FFNL-QEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMY 242
            +++ + I       P D  GHG+HT+ST AG  V  A+LYG+  GTARGGVP ARIA Y
Sbjct: 187 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 246

Query: 243 KVCWNVGCTDADLLAGFDHAIADGVDIISVSIGG-GLREFFEDPIAIGSFHAMAKGILTS 302
           KVCWN GC+D D+LA +D AIADGVDIIS+S+GG   R +F D IAIGSFHA+ +GILTS
Sbjct: 247 KVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 306

Query: 303 CSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYP 362
            SAGN GP   T  + +PW+++VAAST+DR F T +++GN +   G+S+NTF    + YP
Sbjct: 307 NSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYP 366

Query: 363 LISGGSAALPN----HPNARWCDGGSLDK-------------------VSELGGVGVIAN 422
           L+SG    +PN       +R+C   S++                       L G   +  
Sbjct: 367 LVSGRD--IPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 426

Query: 423 LMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTT-TKTEAPFLAYFSSR 432
             N  +     P+P++ L   D      YI SI+ P A I K+TT     AP +  FSSR
Sbjct: 427 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480

[Close]

BLAST of CmaCh06G004620 vs. ExPASy Swiss-Prot
Match: Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.1e-80
Identity = 196/454 (43.17%), Postives = 253/454 (55.73%), Query Frame = 0


Query: 10  LSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKI 69
           +SF+   +AV       ++ YVVYMG+LP  +       +H S+L    G+  +  +  +
Sbjct: 16  VSFVISVSAVTDD-SQDKQVYVVYMGSLPSSRLEYTPMSHHMSILQEVTGESSVEGR-LV 75

Query: 70  YSYGRSFNGFAAMLLPHEAQKLAMAA-----------------------------SRQNA 129
            SY RSFNGFAA L   E +++A                                +++N 
Sbjct: 76  RSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKRNL 135

Query: 130 ATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQ 189
           A ESD I+G +DSGIW ES SF D G+G  P K KG C  G NFT CN K+IGAR +   
Sbjct: 136 AIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFT-CNNKLIGARDYT-- 195

Query: 190 EIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNV 249
                 N+   D  GHG+HT+ST AG  V   S YGI  GTARGGVPA+RIA YK C  +
Sbjct: 196 ------NEGTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAYKACSEM 255

Query: 250 GCTDADLLAGFDHAIADGVDIISVSIGGGL-REFFEDPIAIGSFHAMAKGILTSCSAGNN 309
           GCT   +L+ FD AIADGVD+IS+S+G  L R +  DPIAIG+FHAM KGILT  SAGN 
Sbjct: 256 GCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTVQSAGNG 315

Query: 310 GPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGS 369
           GP   +V + APWI+TVAAS  +R F T + LGN K   G SLN F  K K YPL  G +
Sbjct: 316 GPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYPLYGGST 375

Query: 370 AALPNHPNARWCDGGSLDKVSELGGVGVIANLMNNM-EVVTVTPIPATHLSSQDSDIVEA 429
               + P  R     S DKVS      V+AN+  N  +   V+ +P++ LS  D D V +
Sbjct: 376 ----DGPLLRGKILVSEDKVS---SEIVVANINENYHDYAYVSILPSSALSKDDFDSVIS 435

Query: 430 YINSIKKPKAVIMKTTTTKTE-APFLAYFSSRGP 432
           Y+NS K P   ++K+     + AP +A FSSRGP
Sbjct: 436 YVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGP 451

[Close]

BLAST of CmaCh06G004620 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.1e-80
Identity = 197/472 (41.74%), Postives = 259/472 (54.87%), Query Frame = 0


Query: 10  LSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKI 69
           L+FI      A+        Y+VYMG LP+++ Y     +H S+L   VG    A    +
Sbjct: 13  LAFIFTRDVSANDYRQASSVYIVYMGTLPEIK-YSP-PSHHLSILQKLVG-TIAASHLLV 72

Query: 70  YSYGRSFNGFAAMLLPHEAQKLAM---------------------------AASRQNAAT 129
            SY RSFNGFAA L   E+QKL                               +R+ +  
Sbjct: 73  RSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVK 132

Query: 130 ESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQEI 189
           ESD+IVG++DSGIW ES SF D+G+G  P K KG C  G  F  CN K+IGARF+N    
Sbjct: 133 ESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFYN---- 192

Query: 190 DATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNVGC 249
                 S  DE GHG+HT+ST AG  V  AS YG+A GTARGGVP+ARIA YKVC+N  C
Sbjct: 193 --KFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RC 252

Query: 250 TDADLLAGFDHAIADGVDIISVSIGGG-LREFFEDPIAIGSFHAMAKGILTSCSAGNNGP 309
            D D+LA FD AIADGVD+IS+SI    +       +AIGSFHAM +GI+T+ SAGNNGP
Sbjct: 253 NDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGP 312

Query: 310 GLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSAA 369
              +V N +PW++TVAAS  DR F   + LGN K L+GIS+NTF+     +P++ G + +
Sbjct: 313 DQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVS 372

Query: 370 LP-NHPNARWCDGGSLDKVSEL--------------------GGVGVIANLMNNMEVVTV 429
              +   A +C  G +D  SEL                    G +GVI       +   V
Sbjct: 373 RNCSQAQAGYCSSGCVD--SELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFV 432

Query: 430 TPIPATHLSSQDSDIVEAYINSIKKPKAVIMKT-TTTKTEAPFLAYFSSRGP 432
            P PA+ L  +D   +++YI S + P+A I++T      EAP++  FSSRGP
Sbjct: 433 VPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGP 471

[Close]

BLAST of CmaCh06G004620 vs. ExPASy TrEMBL
Match: A0A6J1GF98 (subtilisin-like protease SBT4.15 OS=Cucurbita moschata OX=3662 GN=LOC111453633 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.7e-223
Identity = 409/478 (85.56%), Postives = 417/478 (87.24%), Query Frame = 0


Query: 1   MNYKMLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGD 60
           MNYKMLK FLSFISL AAVASS  +PRKAYVVYMGALPKVQS+GVLEEYHHSLLANAVGD
Sbjct: 1   MNYKMLKGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGD 60

Query: 61  EELARKSKIYSYGRSFNGFAAMLLPHEAQKLA---------------------------- 120
           EELARKSKIYSYGRSFNGFAA LLPHEAQKLA                            
Sbjct: 61  EELARKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKSVVSVFRSTIRKLHTTRSWDFLGLS 120

Query: 121 MAASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVI 180
            AASR+NAA ESD+IVGLLDSGIWM SPSFKDDGYGEIPSK KGHCVTGHNFTGCNRKVI
Sbjct: 121 AAASRRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVI 180

Query: 181 GARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240
           GARFFNLQEIDATINQSP DEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA
Sbjct: 181 GARFFNLQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240

Query: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300
           MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT
Sbjct: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300

Query: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMY 360
           SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSGISLNTFSTKKKMY
Sbjct: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMY 360

Query: 361 PLISGGSAALPNHPNARWCDGGSLDK-------------------VSELGGVGVIANLMN 420
           PLISGGSAALPNHPNARWCDGGSLDK                   VSELGG GVIANLMN
Sbjct: 361 PLISGGSAALPNHPNARWCDGGSLDKGKVEGKILYCLGSLDQEYTVSELGGAGVIANLMN 420

Query: 421 NMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRGP 432
           N EV+ VTPIPATHLSSQDSDIVEAYIN+ +KPKAVIMKTTTTKTEAPFLAYFSSRGP
Sbjct: 421 NTEVLAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGP 478

[Close]

BLAST of CmaCh06G004620 vs. ExPASy TrEMBL
Match: A0A5A7TQ04 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G002370 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-179
Identity = 336/481 (69.85%), Postives = 377/481 (78.38%), Query Frame = 0


Query: 1   MNYKMLK--AFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAV 60
           MNY+MLK    L+ I LFAA  S+    R+AYVVYMGALPK++S  VL ++HH LLANAV
Sbjct: 1   MNYQMLKLPLILTIIFLFAAAVSATNADRQAYVVYMGALPKLKSPEVLADHHHGLLANAV 60

Query: 61  GDEELARKSKIYSYGRSFNGFAAMLLPHEAQKLAM------------------------A 120
           GDEE+ARK+KIYSYGRSFNGFAA LLPHEA KLAM                        A
Sbjct: 61  GDEEMARKAKIYSYGRSFNGFAARLLPHEADKLAMVSVFRSKTRKLHTTRSWDFLGLSEA 120

Query: 121 ASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGA 180
           ASR+NAA ES++IVGLLDSGIWME PSFKDDGYGEIPSK KG CVTGHNFT CNRKVIGA
Sbjct: 121 ASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRKVIGA 180

Query: 181 RFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMY 240
           RFF+++EID + N+SP DE+GHGSHT+ST+AGA VDGASLYG+AGG ARGGVPAARIAMY
Sbjct: 181 RFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAARIAMY 240

Query: 241 KVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSC 300
           KVCW VGC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAM KGILTSC
Sbjct: 241 KVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGILTSC 300

Query: 301 SAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPL 360
           SAGN+GP L TVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSG+S+NTF+ KK+MYPL
Sbjct: 301 SAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQMYPL 360

Query: 361 ISGGSAALPNHPN-----ARWCDGGSLDK-------------------VSELGGVGVIAN 420
           ISG +AALPN  +       WCD GSLD+                   +S+LGGVGVI+N
Sbjct: 361 ISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVGVISN 420

Query: 421 LMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRG 432
           L+N  E+   TPIP+THLSS +SD VEAYINS K PKAVI KTTT K +APFLAYFSS+G
Sbjct: 421 LLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYFSSKG 480

[Close]

BLAST of CmaCh06G004620 vs. ExPASy TrEMBL
Match: A0A5D3DPI9 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003690 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 4.0e-179
Identity = 335/481 (69.65%), Postives = 376/481 (78.17%), Query Frame = 0


Query: 1   MNYKMLK--AFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAV 60
           MNY+MLK    L+ I LFAA  S+    R+AYVVYMGALPK++S  VL ++HH LLANAV
Sbjct: 1   MNYQMLKLPLILTIIFLFAAAVSATNADRQAYVVYMGALPKLKSPEVLADHHHGLLANAV 60

Query: 61  GDEELARKSKIYSYGRSFNGFAAMLLPHEAQKLAM------------------------A 120
           GDEE+ARK+KIYSYGRSFNGFAA LLPHE  KLAM                        A
Sbjct: 61  GDEEMARKAKIYSYGRSFNGFAARLLPHEVDKLAMVSVFRSKTRKLHTTRSWDFLGLSEA 120

Query: 121 ASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGA 180
           ASR+NAA ES++IVGLLDSGIWME PSFKDDGYGEIPSK KG CVTGHNFT CNRKVIGA
Sbjct: 121 ASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRKVIGA 180

Query: 181 RFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMY 240
           RFF+++EID + N+SP DE+GHGSHT+ST+AGA VDGASLYG+AGG ARGGVPAARIAMY
Sbjct: 181 RFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAARIAMY 240

Query: 241 KVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSC 300
           KVCW VGC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAM KGILTSC
Sbjct: 241 KVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGILTSC 300

Query: 301 SAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPL 360
           SAGN+GP L TVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSG+S+NTF+ KK+MYPL
Sbjct: 301 SAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQMYPL 360

Query: 361 ISGGSAALPNHPN-----ARWCDGGSLDK-------------------VSELGGVGVIAN 420
           ISG +AALPN  +       WCD GSLD+                   +S+LGGVGVI+N
Sbjct: 361 ISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVGVISN 420

Query: 421 LMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRG 432
           L+N  E+   TPIP+THLSS +SD VEAYINS K PKAVI KTTT K +APFLAYFSS+G
Sbjct: 421 LLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYFSSKG 480

[Close]

BLAST of CmaCh06G004620 vs. ExPASy TrEMBL
Match: A0A1S3B6M5 (subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103486592 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 3.1e-147
Identity = 270/365 (73.97%), Postives = 302/365 (82.74%), Query Frame = 0


Query: 91  LAMAASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRK 150
           L+ AASR+NAA ES++IVGLLDSGIWME PSFKDDGYGEIPSK KG CVTGHNFT CNRK
Sbjct: 40  LSEAASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRK 99

Query: 151 VIGARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAAR 210
           VIGARFF+++EID + N+SP DE+GHGSHT+ST+AGA VDGASLYG+AGG ARGGVPAAR
Sbjct: 100 VIGARFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAAR 159

Query: 211 IAMYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGI 270
           IAMYKVCW VGC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAM KGI
Sbjct: 160 IAMYKVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGI 219

Query: 271 LTSCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKK 330
           LTSCSAGN+GP L TVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSG+S+NTF+ KK+
Sbjct: 220 LTSCSAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQ 279

Query: 331 MYPLISGGSAALPNHPN-----ARWCDGGSLDK-------------------VSELGGVG 390
           MYPLISG +AALPN  +       WCD GSLD+                   +S+LGGVG
Sbjct: 280 MYPLISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVG 339

Query: 391 VIANLMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYF 432
           VI+NL+N  E+   TPIP+THLSS +SD VEAYINS K PKAVI KTTT K +APFLAYF
Sbjct: 340 VISNLLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYF 399

[Close]

BLAST of CmaCh06G004620 vs. ExPASy TrEMBL
Match: A0A0A0LE00 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G775270 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 6.1e-143
Identity = 263/364 (72.25%), Postives = 297/364 (81.59%), Query Frame = 0


Query: 91  LAMAASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRK 150
           L+ A SR+NAA ES++IVGLLDSGIWME PSFKDDGYGEIPSK KG CVTG NFT CNRK
Sbjct: 36  LSEAVSRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGRNFTSCNRK 95

Query: 151 VIGARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAAR 210
           VIGARFF++ +ID +I++SP DE+GHGSHT+ST+AGA+VDGAS YG+AGGTARGGVP AR
Sbjct: 96  VIGARFFDIGQIDNSIDKSPADEIGHGSHTASTIAGASVDGASFYGVAGGTARGGVPGAR 155

Query: 211 IAMYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGI 270
           IAMYKVCW  GC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAM KGI
Sbjct: 156 IAMYKVCWVDGCSDVDLLAGFDHAIADGVDIISVSIGGESTEFFNDPIAIGSFHAMEKGI 215

Query: 271 LTSCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKK 330
           LTSCSAGN+GP L TVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSG+S+NTF+ KK+
Sbjct: 216 LTSCSAGNSGPELKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQ 275

Query: 331 MYPLISGGSAALPN----HPNARWCDGGSLDK-------------------VSELGGVGV 390
           MYPLISG +AALPN    + +  WCD G+LD+                   +SELGG GV
Sbjct: 276 MYPLISGSNAALPNQSDPYLDPSWCDSGTLDEKKVKGKIVYCLGSMDQEYTISELGGKGV 335

Query: 391 IANLMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFS 432
           I+NLMN  E    TPIP+THLSS +SD VEAYINS K PKAVI KTTT K +AP+LA FS
Sbjct: 336 ISNLMNVSETAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPYLASFS 395

[Close]

BLAST of CmaCh06G004620 vs. NCBI nr
Match: XP_022950578.1 (subtilisin-like protease SBT4.15 [Cucurbita moschata])

HSP 1 Score: 784.3 bits (2024), Expect = 5.5e-223
Identity = 409/478 (85.56%), Postives = 417/478 (87.24%), Query Frame = 0


Query: 1   MNYKMLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGD 60
           MNYKMLK FLSFISL AAVASS  +PRKAYVVYMGALPKVQS+GVLEEYHHSLLANAVGD
Sbjct: 1   MNYKMLKGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGD 60

Query: 61  EELARKSKIYSYGRSFNGFAAMLLPHEAQKLA---------------------------- 120
           EELARKSKIYSYGRSFNGFAA LLPHEAQKLA                            
Sbjct: 61  EELARKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKSVVSVFRSTIRKLHTTRSWDFLGLS 120

Query: 121 MAASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVI 180
            AASR+NAA ESD+IVGLLDSGIWM SPSFKDDGYGEIPSK KGHCVTGHNFTGCNRKVI
Sbjct: 121 AAASRRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVI 180

Query: 181 GARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240
           GARFFNLQEIDATINQSP DEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA
Sbjct: 181 GARFFNLQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240

Query: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300
           MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT
Sbjct: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300

Query: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMY 360
           SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSGISLNTFSTKKKMY
Sbjct: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMY 360

Query: 361 PLISGGSAALPNHPNARWCDGGSLDK-------------------VSELGGVGVIANLMN 420
           PLISGGSAALPNHPNARWCDGGSLDK                   VSELGG GVIANLMN
Sbjct: 361 PLISGGSAALPNHPNARWCDGGSLDKGKVEGKILYCLGSLDQEYTVSELGGAGVIANLMN 420

Query: 421 NMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRGP 432
           N EV+ VTPIPATHLSSQDSDIVEAYIN+ +KPKAVIMKTTTTKTEAPFLAYFSSRGP
Sbjct: 421 NTEVLAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGP 478

[Close]

BLAST of CmaCh06G004620 vs. NCBI nr
Match: KAG7028095.1 (Subtilisin-like protease SBT4.15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 775.0 bits (2000), Expect = 3.4e-220
Identity = 405/474 (85.44%), Postives = 414/474 (87.34%), Query Frame = 0


Query: 5   MLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELA 64
           MLK FLSFISL AAVASS  +PRKAYVVYMGALPKVQS+GVLEEYHHSLLANAVGDEELA
Sbjct: 1   MLKGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGDEELA 60

Query: 65  RKSKIYSYGRSFNGFAAMLLPHEAQKLA----------------------------MAAS 124
           RKSKIYSYGRSFNGFAA LLPHEAQKLA                             AAS
Sbjct: 61  RKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKGVVSVFQSTTRKLHTTRSWDFLGLSTAAS 120

Query: 125 RQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARF 184
           R+NAA ESD+IVGLLDSGIWM SPSFKDDGYGEIPSK KGHCVTGHNFTGCNRKVIGARF
Sbjct: 121 RRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVIGARF 180

Query: 185 FNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKV 244
           FNLQEIDATINQSP DEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKV
Sbjct: 181 FNLQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKV 240

Query: 245 CWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSA 304
           CWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSA
Sbjct: 241 CWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSA 300

Query: 305 GNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLIS 364
           GNNGPGLTTVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSGISLNTFSTKKKMYPLIS
Sbjct: 301 GNNGPGLTTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMYPLIS 360

Query: 365 GGSAALPNHPNARWCDGGSLDK-------------------VSELGGVGVIANLMNNMEV 424
           GGSAALPNHPNARWCDGGSLDK                   VSELGGVGVIANLMN+ EV
Sbjct: 361 GGSAALPNHPNARWCDGGSLDKGKVEGKILYCLGSLDQEYTVSELGGVGVIANLMNSTEV 420

Query: 425 VTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRGP 432
           + VTPIPATHLSSQDSDIVEAYIN+ +KPKAVIMKTTTTKTEAPFLAYFSSRGP
Sbjct: 421 LAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGP 474

[Close]

BLAST of CmaCh06G004620 vs. NCBI nr
Match: XP_023539892.1 (subtilisin-like protease SBT4.15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.0 bits (2000), Expect = 3.4e-220
Identity = 404/478 (84.52%), Postives = 414/478 (86.61%), Query Frame = 0


Query: 1   MNYKMLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGD 60
           MNYKMLK FLSFISL AAVASS  VPRKAYVVYMGALPKVQS+GVLE+YHHSLLANAVGD
Sbjct: 1   MNYKMLKGFLSFISLIAAVASSTHVPRKAYVVYMGALPKVQSHGVLEQYHHSLLANAVGD 60

Query: 61  EELARKSKIYSYGRSFNGFAAMLLPHEAQKLA---------------------------- 120
           EELARKSKIYSYGRSFNGFAA LLPHEAQKLA                            
Sbjct: 61  EELARKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKGVVSVFQSTTRKLHTTRSWDFLGLS 120

Query: 121 MAASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVI 180
           +AAS +NAA ESD+IVGLLDSGIWM SPSFKDDGYGEIPSK KGHCVTGHNFTGCNRKVI
Sbjct: 121 VAASHRNAAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVI 180

Query: 181 GARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240
           GARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA
Sbjct: 181 GARFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIA 240

Query: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300
           MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT
Sbjct: 241 MYKVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILT 300

Query: 301 SCSAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMY 360
           SCSAGNNGPGLTTVEN+APWIMTVAASTIDRDFSTV+KLGNNKK  GISLNTFSTKKKM 
Sbjct: 301 SCSAGNNGPGLTTVENSAPWIMTVAASTIDRDFSTVVKLGNNKKFQGISLNTFSTKKKMS 360

Query: 361 PLISGGSAALPNHPNARWCDGGSLDK-------------------VSELGGVGVIANLMN 420
           PLISGG AALPNHPNARWCDGGSLDK                   +SELGGVGVIANLMN
Sbjct: 361 PLISGGRAALPNHPNARWCDGGSLDKGKVKGKILYCLGSLDQEYTISELGGVGVIANLMN 420

Query: 421 NMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRGP 432
           N EV+ VTPIPATHLSSQDSDIVE YINS +KPKAVIMKTTTTKTEAPFLAYFSSRGP
Sbjct: 421 NTEVLAVTPIPATHLSSQDSDIVETYINSTQKPKAVIMKTTTTKTEAPFLAYFSSRGP 478

[Close]

BLAST of CmaCh06G004620 vs. NCBI nr
Match: KAG6596559.1 (Subtilisin-like protease 4.15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 771.2 bits (1990), Expect = 4.8e-219
Identity = 403/474 (85.02%), Postives = 412/474 (86.92%), Query Frame = 0


Query: 5   MLKAFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELA 64
           MLK FLSFISL AAVASS  +PRKAYVVYMGALPKVQS+GVLEEYHHSLLANAVGDEELA
Sbjct: 1   MLKGFLSFISLIAAVASSTNIPRKAYVVYMGALPKVQSHGVLEEYHHSLLANAVGDEELA 60

Query: 65  RKSKIYSYGRSFNGFAAMLLPHEAQKLA----------------------------MAAS 124
           RKSKIYSYGRSFNGFAA LLPHEAQKLA                             AAS
Sbjct: 61  RKSKIYSYGRSFNGFAAKLLPHEAQKLAKEKSVVSVFRSTIRKLHTTRSWDFLGLSAAAS 120

Query: 125 RQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARF 184
           R+N A ESD+IVGLLDSGIWM SPSFKDDGYGEIPSK KGHCVTGHNFTGCNRKVIGARF
Sbjct: 121 RRNTAAESDMIVGLLDSGIWMGSPSFKDDGYGEIPSKWKGHCVTGHNFTGCNRKVIGARF 180

Query: 185 FNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKV 244
           FNLQEIDATINQSP DEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKV
Sbjct: 181 FNLQEIDATINQSPADEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKV 240

Query: 245 CWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSA 304
           CWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSA
Sbjct: 241 CWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSA 300

Query: 305 GNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLIS 364
           GNNGPGL TVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSGISLNTFSTKKKMYPLIS
Sbjct: 301 GNNGPGLRTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGISLNTFSTKKKMYPLIS 360

Query: 365 GGSAALPNHPNARWCDGGSLDK-------------------VSELGGVGVIANLMNNMEV 424
           GGSAALPNHPNARWCDGGSLDK                   VSELGGVGVIANLMN+ EV
Sbjct: 361 GGSAALPNHPNARWCDGGSLDKVKVEGKILYCLGSLDQEYTVSELGGVGVIANLMNSTEV 420

Query: 425 VTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRGP 432
           + VTPIPATHLSSQDSDIVEAYIN+ +KPKAVIMKTTTTKTEAPFLAYFSSRGP
Sbjct: 421 LAVTPIPATHLSSQDSDIVEAYINATQKPKAVIMKTTTTKTEAPFLAYFSSRGP 474

[Close]

BLAST of CmaCh06G004620 vs. NCBI nr
Match: KAA0043897.1 (subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa])

HSP 1 Score: 639.0 bits (1647), Expect = 2.9e-179
Identity = 336/481 (69.85%), Postives = 377/481 (78.38%), Query Frame = 0


Query: 1   MNYKMLK--AFLSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAV 60
           MNY+MLK    L+ I LFAA  S+    R+AYVVYMGALPK++S  VL ++HH LLANAV
Sbjct: 1   MNYQMLKLPLILTIIFLFAAAVSATNADRQAYVVYMGALPKLKSPEVLADHHHGLLANAV 60

Query: 61  GDEELARKSKIYSYGRSFNGFAAMLLPHEAQKLAM------------------------A 120
           GDEE+ARK+KIYSYGRSFNGFAA LLPHEA KLAM                        A
Sbjct: 61  GDEEMARKAKIYSYGRSFNGFAARLLPHEADKLAMVSVFRSKTRKLHTTRSWDFLGLSEA 120

Query: 121 ASRQNAATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGA 180
           ASR+NAA ES++IVGLLDSGIWME PSFKDDGYGEIPSK KG CVTGHNFT CNRKVIGA
Sbjct: 121 ASRRNAAAESNVIVGLLDSGIWMEGPSFKDDGYGEIPSKWKGKCVTGHNFTSCNRKVIGA 180

Query: 181 RFFNLQEIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMY 240
           RFF+++EID + N+SP DE+GHGSHT+ST+AGA VDGASLYG+AGG ARGGVPAARIAMY
Sbjct: 181 RFFDIEEIDNSNNKSPVDEIGHGSHTASTIAGAFVDGASLYGVAGGKARGGVPAARIAMY 240

Query: 241 KVCWNVGCTDADLLAGFDHAIADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSC 300
           KVCW VGC+D DLLAGFDHAIADGVDIISVSIGG   EFF DPIAIGSFHAM KGILTSC
Sbjct: 241 KVCWAVGCSDVDLLAGFDHAIADGVDIISVSIGGESMEFFNDPIAIGSFHAMEKGILTSC 300

Query: 301 SAGNNGPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPL 360
           SAGN+GP L TVENTAPWIMTVAASTIDRDFSTV+KLGNNKKLSG+S+NTF+ KK+MYPL
Sbjct: 301 SAGNSGPDLKTVENTAPWIMTVAASTIDRDFSTVVKLGNNKKLSGVSVNTFTPKKQMYPL 360

Query: 361 ISGGSAALPNHPN-----ARWCDGGSLDK-------------------VSELGGVGVIAN 420
           ISG +AALPN  +       WCD GSLD+                   +S+LGGVGVI+N
Sbjct: 361 ISGSNAALPNQSSDLDLYPSWCDYGSLDEKKVKGKIVYCLGSIDQEYTISDLGGVGVISN 420

Query: 421 LMNNMEVVTVTPIPATHLSSQDSDIVEAYINSIKKPKAVIMKTTTTKTEAPFLAYFSSRG 432
           L+N  E+   TPIP+THLSS +SD VEAYINS K PKAVI KTTT K +APFLAYFSS+G
Sbjct: 421 LLNITEMAITTPIPSTHLSSTNSDYVEAYINSTKNPKAVIYKTTTRKVDAPFLAYFSSKG 480

[Close]

BLAST of CmaCh06G004620 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 411.0 bits (1055), Expect = 1.2e-114
Identity = 232/467 (49.68%), Postives = 291/467 (62.31%), Query Frame = 0


Query: 25  VPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSYGRSFNGFAAMLL 84
           V RK Y+VYMG   +  S     E HH+LL   +GDE  AR+ KIYSYG++ NGF A L 
Sbjct: 30  VERKPYIVYMGEATE-NSLVEAAENHHNLLMTVIGDESKARELKIYSYGKNINGFVARLF 89

Query: 85  PHEAQKLAM----------------------------AASRQNAATESDIIVGLLDSGIW 144
           PHEA+KL+                             +  +++   ES+IIVG+LD+GI 
Sbjct: 90  PHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGID 149

Query: 145 MESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQEIDATINQSPT--DEV 204
           +ESPSF D G G  P+K KG CVTG+NFT CN KVIGA++F++Q       +  T  D  
Sbjct: 150 VESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGDTAADHD 209

Query: 205 GHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNVGCTDADLLAGFDHA 264
           GHG+HTSST+AG +V  ASL+GIA GTARGGVP+ARIA YKVCW+ GCTD D+LA FD A
Sbjct: 210 GHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEA 269

Query: 265 IADGVDIISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGNNGPGLTTVENTAPWIM 324
           I+DGVDIIS+SIGG    FFEDPIAIG+FHAM +GILT+CSAGNNGPGL TV N APW+M
Sbjct: 270 ISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVM 329

Query: 325 TVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSA---ALPNHPNARWC 384
           TVAA+++DR F TV+KLGN    SGISLN F+ +KKMYPL SG  A   +   +     C
Sbjct: 330 TVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPSTC 389

Query: 385 DGGSL--DK-------------------------VSELGGVGVIANLMNNMEVVTVTPIP 432
           + G+L  DK                         V  L G GVI  L+   ++ T T I 
Sbjct: 390 EPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIA 449

[Close]

BLAST of CmaCh06G004620 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 337.8 bits (865), Expect = 1.3e-92
Identity = 201/453 (44.37%), Postives = 263/453 (58.06%), Query Frame = 0


Query: 30  YVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSYGRSFNGFAAMLLPHEAQ 89
           Y++Y+G  P      +  + H +LL++    +E A++ K+YSY ++FN FAA L PHEA+
Sbjct: 38  YIIYLGDRPDNTEETI--KTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAK 97

Query: 90  KL-----AMAASR----------------------QNAATESDIIVGLLDSGIWMESPSF 149
           K+      ++ SR                      ++   E D+I+G+LD+GI  +S SF
Sbjct: 98  KMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESF 157

Query: 150 KDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQ-EIDATINQSPTDEVGHGSHTS 209
            D G G  P+K KG C    NFTGCN K+IGA++F     + A   +SP D  GHG+HTS
Sbjct: 158 LDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTS 217

Query: 210 STVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCW-NVGCTDADLLAGFDHAIADGVD 269
           STVAG  V  ASLYGIA GTARG VP+AR+AMYKVCW   GC D D+LAGF+ AI DGV+
Sbjct: 218 STVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVE 277

Query: 270 IISVSIGGGLREFFEDPIAIGSFHAMAKGILTSCSAGNNGPGLTTVENTAPWIMTVAAST 329
           IIS+SIGG + ++  D I++GSFHAM KGILT  SAGN+GP   TV N  PWI+TVAAS 
Sbjct: 278 IISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASG 337

Query: 330 IDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSAALPNHPN--ARWCDGGSLDK 389
           IDR F + + LGN K  SG+ ++ FS K K YPL+SG  AA        AR+C   SLD+
Sbjct: 338 IDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDR 397

Query: 390 --------------------VSELGGVGVIANLMNNMEVVTVTPIPATHLSSQDSDIVEA 432
                               +   GG G I      ++   +   PAT ++S   DI+  
Sbjct: 398 KKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYR 457

[Close]

BLAST of CmaCh06G004620 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 302.0 bits (772), Expect = 7.8e-82
Identity = 196/454 (43.17%), Postives = 253/454 (55.73%), Query Frame = 0


Query: 10  LSFISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKI 69
           +SF+   +AV       ++ YVVYMG+LP  +       +H S+L    G+  +  +  +
Sbjct: 16  VSFVISVSAVTDD-SQDKQVYVVYMGSLPSSRLEYTPMSHHMSILQEVTGESSVEGR-LV 75

Query: 70  YSYGRSFNGFAAMLLPHEAQKLAMAA-----------------------------SRQNA 129
            SY RSFNGFAA L   E +++A                                +++N 
Sbjct: 76  RSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKRNL 135

Query: 130 ATESDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQ 189
           A ESD I+G +DSGIW ES SF D G+G  P K KG C  G NFT CN K+IGAR +   
Sbjct: 136 AIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFT-CNNKLIGARDYT-- 195

Query: 190 EIDATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNV 249
                 N+   D  GHG+HT+ST AG  V   S YGI  GTARGGVPA+RIA YK C  +
Sbjct: 196 ------NEGTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAYKACSEM 255

Query: 250 GCTDADLLAGFDHAIADGVDIISVSIGGGL-REFFEDPIAIGSFHAMAKGILTSCSAGNN 309
           GCT   +L+ FD AIADGVD+IS+S+G  L R +  DPIAIG+FHAM KGILT  SAGN 
Sbjct: 256 GCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTVQSAGNG 315

Query: 310 GPGLTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGS 369
           GP   +V + APWI+TVAAS  +R F T + LGN K   G SLN F  K K YPL  G +
Sbjct: 316 GPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYPLYGGST 375

Query: 370 AALPNHPNARWCDGGSLDKVSELGGVGVIANLMNNM-EVVTVTPIPATHLSSQDSDIVEA 429
               + P  R     S DKVS      V+AN+  N  +   V+ +P++ LS  D D V +
Sbjct: 376 ----DGPLLRGKILVSEDKVS---SEIVVANINENYHDYAYVSILPSSALSKDDFDSVIS 435

Query: 430 YINSIKKPKAVIMKTTTTKTE-APFLAYFSSRGP 432
           Y+NS K P   ++K+     + AP +A FSSRGP
Sbjct: 436 YVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGP 451

[Close]

BLAST of CmaCh06G004620 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 299.3 bits (765), Expect = 5.0e-81
Identity = 194/467 (41.54%), Postives = 258/467 (55.25%), Query Frame = 0


Query: 13  ISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSY 72
           + L ++V++ I    + Y+VYMG+L     Y +    H S+L    G+  +  +  + SY
Sbjct: 16  VLLLSSVSAIIDEDTQVYIVYMGSLSSRADY-IPTSDHMSILQQVTGESSIEGR-LVRSY 75

Query: 73  GRSFNGFAAMLLPHEAQKLAMAA---------------------------SRQNAATESD 132
            RSFNGFAA L   E   +A                              +++N A ESD
Sbjct: 76  KRSFNGFAARLTESERTLIAEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESD 135

Query: 133 IIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQEIDAT 192
            I+G++D+GIW ES SF D G+G  P K KG C  G NFT CN K+IGAR +        
Sbjct: 136 TIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT-CNNKLIGARDYT------- 195

Query: 193 INQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNVGCTDA 252
            ++   D  GHG+HT+ST AG  V   S +GI  GT RGGVPA+RIA YKVC + GC+  
Sbjct: 196 -SEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSE 255

Query: 253 DLLAGFDHAIADGVDIISVSIGGGLREFFE-DPIAIGSFHAMAKGILTSCSAGNNGPGLT 312
            LL+ FD AIADGVD+I++SIG      FE DPIAIG+FHAMAKGILT  SAGN+GP  T
Sbjct: 256 ALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPT 315

Query: 313 TVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSAALP- 372
           TV + APWI TVAAST +R F T + LGN K L+G S+N F  K K YPL+ G SAA   
Sbjct: 316 TVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSA 375

Query: 373 -NHPNARWCDGGSLDKVSELGGVGV--------IANLMNNMEVVTVTP---------IPA 432
            +   A  C    L+K    G + V        IA  +  + ++  +P         +PA
Sbjct: 376 CDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPA 435

[Close]

BLAST of CmaCh06G004620 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 297.7 bits (761), Expect = 1.5e-80
Identity = 194/469 (41.36%), Postives = 258/469 (55.01%), Query Frame = 0


Query: 13  ISLFAAVASSIPVPRKAYVVYMGALPKVQSYGVLEEYHHSLLANAVGDEELARKSKIYSY 72
           + L ++V++ I    + Y+VYMG+L     Y +    H S+L    G+  +  +  + SY
Sbjct: 16  VLLLSSVSAIIDEDTQVYIVYMGSLSSRADY-IPTSDHMSILQQVTGESSIEGR-LVRSY 75

Query: 73  GRSFNGFAAMLLPHEAQKLAMAA-----------------------------SRQNAATE 132
            RSFNGFAA L   E   +A                                +++N A E
Sbjct: 76  KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 135

Query: 133 SDIIVGLLDSGIWMESPSFKDDGYGEIPSKGKGHCVTGHNFTGCNRKVIGARFFNLQEID 192
           SD I+G++D+GIW ES SF D G+G  P K KG C  G NFT CN K+IGAR +      
Sbjct: 136 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT-CNNKLIGARDYT----- 195

Query: 193 ATINQSPTDEVGHGSHTSSTVAGATVDGASLYGIAGGTARGGVPAARIAMYKVCWNVGCT 252
              ++   D  GHG+HT+ST AG  V   S +GI  GT RGGVPA+RIA YKVC + GC+
Sbjct: 196 ---SEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCS 255

Query: 253 DADLLAGFDHAIADGVDIISVSIGGGLREFFE-DPIAIGSFHAMAKGILTSCSAGNNGPG 312
              LL+ FD AIADGVD+I++SIG      FE DPIAIG+FHAMAKGILT  SAGN+GP 
Sbjct: 256 SEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPK 315

Query: 313 LTTVENTAPWIMTVAASTIDRDFSTVMKLGNNKKLSGISLNTFSTKKKMYPLISGGSAAL 372
            TTV + APWI TVAAST +R F T + LGN K L+G S+N F  K K YPL+ G SAA 
Sbjct: 316 PTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAAS 375

Query: 373 P--NHPNARWCDGGSLDKVSELGGVGV--------IANLMNNMEVVTVTP---------I 432
              +   A  C    L+K    G + V        IA  +  + ++  +P         +
Sbjct: 376 SACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRPDVAFTHHL 435

The following BLAST results are available for this feature:

BLAST of CmaCh06G004620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZS6	1.7e-113	49.68	Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3...	[more]
Q9LLL8	1.8e-91	44.37	Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2...	[more]
Q39547	2.6e-82	40.25	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIM5	1.1e-80	43.17	Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 S...	[more]
Q9FIF8	7.1e-80	41.74	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]

BLAST of CmaCh06G004620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GF98	2.7e-223	85.56	subtilisin-like protease SBT4.15 OS=Cucurbita moschata OX=3662 GN=LOC111453633 P...	[more]
A0A5A7TQ04	1.4e-179	69.85	Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3DPI9	4.0e-179	69.65	Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B6M5	3.1e-147	73.97	subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103486592 PE=3 SV...	[more]
A0A0A0LE00	6.1e-143	72.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G775270 PE=3 SV=1	[more]

BLAST of CmaCh06G004620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950578.1	5.5e-223	85.56	subtilisin-like protease SBT4.15 [Cucurbita moschata]	[more]
KAG7028095.1	3.4e-220	85.44	Subtilisin-like protease SBT4.15, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_023539892.1	3.4e-220	84.52	subtilisin-like protease SBT4.15 [Cucurbita pepo subsp. pepo]	[more]
KAG6596559.1	4.8e-219	85.02	Subtilisin-like protease 4.15, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAA0043897.1	2.9e-179	69.85	subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa]	[more]

BLAST of CmaCh06G004620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03620.1	1.2e-114	49.68	Subtilisin-like serine endopeptidase family protein	[more]
AT4G00230.1	1.3e-92	44.37	xylem serine peptidase 1	[more]
AT5G58840.1	7.8e-82	43.17	Subtilase family protein	[more]
AT5G59090.3	5.0e-81	41.54	subtilase 4.12	[more]
AT5G59090.1	1.5e-80	41.36	subtilase 4.12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 104..379 e-value: 3.3E-23 score: 82.3
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 106..431 e-value: 2.1E-107 score: 361.7
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 100..428
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 17..105 e-value: 1.1E-14 score: 56.6
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 30..94 e-value: 1.7E-7 score: 31.8
None	No IPR available	GENE3D	3.50.30.30		coord: 302..416 e-value: 2.1E-107 score: 361.7
None	No IPR available	PANTHER	PTHR10795:SF630	CUCUMISIN PROTEIN	coord: 100..431
None	No IPR available	PANTHER	PTHR10795:SF630	CUCUMISIN PROTEIN	coord: 18..92
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 85..431 score: 17.943497
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 308..410 e-value: 3.1518E-10 score: 55.8827
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 100..431
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 18..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G004620.1	CmaCh06G004620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity