CmaCh06G004170 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G004170
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	purine-uracil permease NCS1-like
Location	Cma_Chr06: 1933796 .. 1934431 (-)
RNA-Seq Expression	CmaCh06G004170
Synteny	CmaCh06G004170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGGCGTTCACTTTCGTCGGCGTTGCTGTCACCTCCTCCACCGGCGTGATTTTTGGGCATGTAATTTCAAACCCAATTGATCTTCTTGCCCATATTGGGGGTTTTACCACTAAGGTTTTGGCGATTTTTGGGATCATTTTGGCCACTGTCACCACCAATGTCGCCGCCAATGTGGTGGCGCCGGCGAATGCGTTGGTCAATCTCAGCCCTTCTTATTTCACTTTCAACAGAGGGGCACTTCTCACCGCCGTGATTGGCATTCTTTTTCAGCCGTGGAAGCTTCCAAGCTCGAGTGAGAGCTTTGTTTACACTTGGCTTGTTGGGTATTCGGCGGTGTTGGGGCCGATTGGCTGCGTCATGGTGGCGGATTACCATTTCATTCGGAAGAGAGAACTGATAGTTGAGGATTTGTATTCTTCCAGTCCGACAGGGGTTTATTATTACTCCGGTGGGTTTAATTTGGCGGCAGTGGCGGCGCTGGTGGTCGGAGTTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATTTTGTCTAATGTTCCTAAAATGTTTAGGGTGATTTACGGGAATGCTTGGTTTATTAGCACCTTTTTCGCCGGATTTTTCTTTTGGGTCTCTCCAATTTGTTGA

mRNA sequence

ATGGGGGCGTTCACTTTCGTCGGCGTTGCTGTCACCTCCTCCACCGGCGTGATTTTTGGGCATGTAATTTCAAACCCAATTGATCTTCTTGCCCATATTGGGGGTTTTACCACTAAGGTTTTGGCGATTTTTGGGATCATTTTGGCCACTGTCACCACCAATGTCGCCGCCAATGTGGTGGCGCCGGCGAATGCGTTGGTCAATCTCAGCCCTTCTTATTTCACTTTCAACAGAGGGGCACTTCTCACCGCCGTGATTGGCATTCTTTTTCAGCCGTGGAAGCTTCCAAGCTCGAGTGAGAGCTTTGTTTACACTTGGCTTGTTGGGTATTCGGCGGTGTTGGGGCCGATTGGCTGCGTCATGGTGGCGGATTACCATTTCATTCGGAAGAGAGAACTGATAGTTGAGGATTTGTATTCTTCCAGTCCGACAGGGGTTTATTATTACTCCGGTGGGTTTAATTTGGCGGCAGTGGCGGCGCTGGTGGTCGGAGTTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATTTTGTCTAATGTTCCTAAAATGTTTAGGGTGATTTACGGGAATGCTTGGTTTATTAGCACCTTTTTCGCCGGATTTTTCTTTTGGGTCTCTCCAATTTGTTGA

Coding sequence (CDS)

ATGGGGGCGTTCACTTTCGTCGGCGTTGCTGTCACCTCCTCCACCGGCGTGATTTTTGGGCATGTAATTTCAAACCCAATTGATCTTCTTGCCCATATTGGGGGTTTTACCACTAAGGTTTTGGCGATTTTTGGGATCATTTTGGCCACTGTCACCACCAATGTCGCCGCCAATGTGGTGGCGCCGGCGAATGCGTTGGTCAATCTCAGCCCTTCTTATTTCACTTTCAACAGAGGGGCACTTCTCACCGCCGTGATTGGCATTCTTTTTCAGCCGTGGAAGCTTCCAAGCTCGAGTGAGAGCTTTGTTTACACTTGGCTTGTTGGGTATTCGGCGGTGTTGGGGCCGATTGGCTGCGTCATGGTGGCGGATTACCATTTCATTCGGAAGAGAGAACTGATAGTTGAGGATTTGTATTCTTCCAGTCCGACAGGGGTTTATTATTACTCCGGTGGGTTTAATTTGGCGGCAGTGGCGGCGCTGGTGGTCGGAGTTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATTTTGTCTAATGTTCCTAAAATGTTTAGGGTGATTTACGGGAATGCTTGGTTTATTAGCACCTTTTTCGCCGGATTTTTCTTTTGGGTCTCTCCAATTTGTTGA

Protein sequence

MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCVMVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILSNVPKMFRVIYGNAWFISTFFAGFFFWVSPIC

Homology

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BLAST of CmaCh06G004170 vs. ExPASy Swiss-Prot
Match: Q9LZD0 (Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 4.5e-72
Identity = 135/207 (65.22%), Postives = 166/207 (80.19%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MG FTFVGVAVTSST +IFG VISNPI+LL  IGG  T +LAI GI LAT+TTN+AANVV
Sbjct: 373 MGLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVV 432

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNL+P +FTF RGA LTAV+GI+FQPW+L  SSESFVYTWL+GYSA+LGPIG +
Sbjct: 433 APANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGI 492

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           ++ DY+ I+K +L + DLYS SP+G YY+S G+N+AAV ALV G++PV+PGFL K+  LS
Sbjct: 493 ILVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALS 552

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFWV 208
            +   F V+Y NA F S   AGF +W+
Sbjct: 553 KISNGFVVVYDNALFFSFIIAGFVYWI 579

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BLAST of CmaCh06G004170 vs. ExPASy Swiss-Prot
Match: P94575 (Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 5.2e-28
Identity = 76/204 (37.25%), Postives = 114/204 (55.88%), Query Frame = 0


Query: 4   FTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVVAPA 63
           F F  + VTS + V FG  I + +D+LA        VL++  + +AT++ NVAAN+V+PA
Sbjct: 275 FAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVIVLSVITLCIATISVNVAANIVSPA 334

Query: 64  NALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCVMVA 123
             + N  P Y  F RG+ +TA++ +   PWKL  S+ S VY +L     +LGP+  VM+A
Sbjct: 335 YDIANALPKYINFKRGSFITALLALFTVPWKLMESATS-VYAFLGLIGGMLGPVAGVMMA 394

Query: 124 DYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILSNVP 183
           DY  IRKREL V+DLYS   TG Y Y  G+N  A AA ++G L         + ++    
Sbjct: 395 DYFIIRKRELSVDDLYSE--TGRYVYWKGYNYRAFAATMLGAL---------ISLIGMYV 454

Query: 184 KMFRVIYGNAWFISTFFAGFFFWV 208
            + + +Y  +WF+    +  F+ V
Sbjct: 455 PVLKSLYDISWFVGVLISFLFYIV 466

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BLAST of CmaCh06G004170 vs. ExPASy Swiss-Prot
Match: Q10279 (Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fur4 PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 2.6e-19
Identity = 67/202 (33.17%), Postives = 104/202 (51.49%), Query Frame = 0


Query: 2   GAFTFVGVAVTSSTGVIFGHVISNPIDLLA------HIGGFTTKVLAI-FGIILATVTTN 61
           G  + +GV V+S++  I+G  + +P  LLA      +  G    V  I FG+ +A +  N
Sbjct: 300 GVTSLIGVLVSSASKAIYGTTLWDPTQLLASFLDHSNAHGVRAGVFFIAFGLCIAQLGVN 359

Query: 62  VAANVVAPANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVL 121
           +AAN V+  N L  L P+     RG  + ++I +   PW L SS+ +F  T+L  YS  L
Sbjct: 360 IAANSVSAGNDLSALLPTVINVRRGGYIASIIALCMCPWNLLSSNNNFT-TYLSSYSVFL 419

Query: 122 GPIGCVMVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLE 181
                V++ADY+F+RK  + V  LYSSS +  YY+  G N  A A+ + G+L  I G   
Sbjct: 420 SSFAGVIIADYYFVRKGLIRVAPLYSSSSSSPYYFWKGINFRAFASYICGMLINIVGMAG 479

Query: 182 KVEILSNVPKMFRVIYGNAWFI 197
                  VPK+   ++   +F+
Sbjct: 480 STG--QKVPKVANTMFNLNYFL 498

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BLAST of CmaCh06G004170 vs. ExPASy Swiss-Prot
Match: O14035 (Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29B12.14c PE=3 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 7.6e-19
Identity = 67/190 (35.26%), Postives = 95/190 (50.00%), Query Frame = 0


Query: 2   GAFTFVGVAVTSSTGVIFGHVISNPIDLLA-------HIGGFTTKVLAIFGIILATVTTN 61
           G  T +GV V S++  I+G  I NP+DLL        H G          G+ LA +  N
Sbjct: 307 GITTLIGVLVGSASKSIYGENIWNPLDLLKSFLDHSNHHGVRAGVFFISTGLCLAQLGVN 366

Query: 62  VAANVVAPANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVL 121
           +AAN V+  N    L P +    RG  + ++IGI   PW L SSS SF  + L  Y+  L
Sbjct: 367 IAANTVSAGNDTSALCPMFINIRRGGYIASIIGICMCPWNLLSSSNSFANS-LSAYAVFL 426

Query: 122 GPIGCVMVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLE 181
                +++ADY  IRK  L V+ LY+ +P   Y+++ G NL A A+ + G+L  + G   
Sbjct: 427 SSFAGILIADYFVIRKGYLKVDALYTINPNEPYWFTYGINLRAFASYICGLLINVVGLAG 486

Query: 182 KVEILSNVPK 185
            V     VPK
Sbjct: 487 AVG--DKVPK 493

[Close]

BLAST of CmaCh06G004170 vs. ExPASy Swiss-Prot
Match: Q2UPA8 (Transporter aclS OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aclS PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 55/186 (29.57%), Postives = 94/186 (50.54%), Query Frame = 0


Query: 8   GVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAAN-VVAPANA- 67
           G   +S+T  I+G  + NP  +LA        V A  G++ + +  NV  N   +P+ + 
Sbjct: 335 GCLTSSATQAIYGEALWNPPTILAISTSRAAAVFAGIGLVSSQLALNVVDNGKSSPSQSV 394

Query: 68  ---------------LVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGY 127
                          L  L P Y    RG  +  ++G+   PW+L +S+ +FV + +  +
Sbjct: 395 KGFSNYPVGYSVGMDLSGLLPKYINIRRGCYVGLILGMALCPWELLASATTFV-SVISSF 454

Query: 128 SAVLGPIGCVMVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIP 177
           S  + P   + ++DY FIR+R L + DLY + P G+Y+Y+ GFN   V   +VG +P++P
Sbjct: 455 SIFMAPFCGIHISDYWFIRQRRLKLSDLYHARPEGIYFYTMGFNWRGVLPWLVGWVPLLP 514

[Close]

BLAST of CmaCh06G004170 vs. ExPASy TrEMBL
Match: A0A6J1KUM8 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498832 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.4e-111
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV
Sbjct: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV
Sbjct: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS
Sbjct: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC 212
           NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC
Sbjct: 181 NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC 211

[Close]

BLAST of CmaCh06G004170 vs. ExPASy TrEMBL
Match: A0A6J1KWR9 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.0e-95
Identity = 183/206 (88.83%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 341 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVV 400

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTF RGALLTAVIGI+FQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 401 APANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 460

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADY+F+RKRELIVEDLYSSSPTG YYYSGGFNL AVAALVVGVLPVIPGFLEKV+I+S
Sbjct: 461 MVADYYFLRKRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVS 520

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VP++F VIYGNAWFIS+F AGF +W
Sbjct: 521 KVPEVFTVIYGNAWFISSFIAGFCYW 546

[Close]

BLAST of CmaCh06G004170 vs. ExPASy TrEMBL
Match: A0A6J1GDF7 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.7e-93
Identity = 180/205 (87.80%), Postives = 194/205 (94.63%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 341 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVV 400

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNL+PSYFTF RGALLTAVIGI+FQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 401 APANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 460

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADY+FIR+RELIVEDLYSSSPTG YY+SGGFNL AVAALVVGVLPVIPGFLEKV+++S
Sbjct: 461 MVADYYFIRRRELIVEDLYSSSPTGAYYFSGGFNLTAVAALVVGVLPVIPGFLEKVKLVS 520

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFF 206
            VP++F VIYGNAWFISTF AGF +
Sbjct: 521 KVPQLFTVIYGNAWFISTFIAGFCY 545

[Close]

BLAST of CmaCh06G004170 vs. ExPASy TrEMBL
Match: A0A5D3DPI8 (Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003170 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.2e-91
Identity = 178/206 (86.41%), Postives = 189/206 (91.75%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 330 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVV 389

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTF RGALLTAVIGILFQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 390 APANALVNLSPSYFTFTRGALLTAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 449

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MV DY+ IR +EL VEDLYS+SP G YYYSGGFNL AV ALVVGVLPVIPGFLEKVEI+S
Sbjct: 450 MVTDYYLIRGKELSVEDLYSTSPRGAYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVS 509

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VPK+F VIYG+AWFIS+F AGF +W
Sbjct: 510 KVPKVFSVIYGSAWFISSFLAGFCYW 535

[Close]

BLAST of CmaCh06G004170 vs. ExPASy TrEMBL
Match: A0A1S3B756 (purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.2e-91
Identity = 178/206 (86.41%), Postives = 189/206 (91.75%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 330 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVV 389

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTF RGALLTAVIGILFQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 390 APANALVNLSPSYFTFTRGALLTAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 449

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MV DY+ IR +EL VEDLYS+SP G YYYSGGFNL AV ALVVGVLPVIPGFLEKVEI+S
Sbjct: 450 MVTDYYLIRGKELSVEDLYSTSPRGAYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVS 509

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VPK+F VIYG+AWFIS+F AGF +W
Sbjct: 510 KVPKVFSVIYGSAWFISSFLAGFCYW 535

[Close]

BLAST of CmaCh06G004170 vs. NCBI nr
Match: XP_023005972.1 (purine-uracil permease NCS1-like [Cucurbita maxima])

HSP 1 Score: 412.1 bits (1058), Expect = 2.8e-111
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV
Sbjct: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV
Sbjct: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS
Sbjct: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC 212
           NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC
Sbjct: 181 NVPKMFRVIYGNAWFISTFFAGFFFWVSPIC 211

[Close]

BLAST of CmaCh06G004170 vs. NCBI nr
Match: KAG6596517.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 357.8 bits (917), Expect = 6.3e-95
Identity = 183/206 (88.83%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 341 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVV 400

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNL+PSYFTF RGALLTAVIGI+FQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 401 APANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 460

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADY+FIR+RELIVEDLYSSSPTG YYYSGGFNL AVAALVVGVLPVIPGFLEKV+I+S
Sbjct: 461 MVADYYFIRRRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVS 520

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VP++F VIYGNAWFISTF AGF +W
Sbjct: 521 KVPQVFTVIYGNAWFISTFIAGFCYW 546

[Close]

BLAST of CmaCh06G004170 vs. NCBI nr
Match: XP_023005190.1 (purine-uracil permease NCS1-like [Cucurbita maxima])

HSP 1 Score: 357.8 bits (917), Expect = 6.3e-95
Identity = 183/206 (88.83%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 341 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVV 400

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTF RGALLTAVIGI+FQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 401 APANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 460

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADY+F+RKRELIVEDLYSSSPTG YYYSGGFNL AVAALVVGVLPVIPGFLEKV+I+S
Sbjct: 461 MVADYYFLRKRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVS 520

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VP++F VIYGNAWFIS+F AGF +W
Sbjct: 521 KVPEVFTVIYGNAWFISSFIAGFCYW 546

[Close]

BLAST of CmaCh06G004170 vs. NCBI nr
Match: KAG7028053.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.8 bits (917), Expect = 6.3e-95
Identity = 183/206 (88.83%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 330 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVV 389

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNL+PSYFTF RGALLTAVIGI+FQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 390 APANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 449

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADY+FIR+RELIVEDLYSSSPTG YYYSGGFNL AVAALVVGVLPVIPGFLEKV+I+S
Sbjct: 450 MVADYYFIRRRELIVEDLYSSSPTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVS 509

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VP++F VIYGNAWFISTF AGF +W
Sbjct: 510 KVPQVFTVIYGNAWFISTFIAGFCYW 535

[Close]

BLAST of CmaCh06G004170 vs. NCBI nr
Match: XP_023539092.1 (purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.1 bits (910), Expect = 4.1e-94
Identity = 182/206 (88.35%), Postives = 193/206 (93.69%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MGAFTFVGVAVTSSTGVIFGH+ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVV
Sbjct: 341 MGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVV 400

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNLSPSYFTF RGALLTAVIGI+FQPW+L SSSESFVYTWLVGYSA+LGPIG V
Sbjct: 401 APANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGV 460

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           MVADY+FIR+RELIVEDLYS SPTG YYYSGGFNL AV ALVVGVLPVIPGFLEKV+I+S
Sbjct: 461 MVADYYFIRRRELIVEDLYSLSPTGAYYYSGGFNLTAVTALVVGVLPVIPGFLEKVKIVS 520

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFW 207
            VP++F VIYGNAWFISTF AGF +W
Sbjct: 521 KVPQVFTVIYGNAWFISTFIAGFCYW 546

[Close]

BLAST of CmaCh06G004170 vs. TAIR 10
Match: AT5G03555.1 (permease, cytosine/purines, uracil, thiamine, allantoin family protein )

HSP 1 Score: 272.3 bits (695), Expect = 3.2e-73
Identity = 135/207 (65.22%), Postives = 166/207 (80.19%), Query Frame = 0


Query: 1   MGAFTFVGVAVTSSTGVIFGHVISNPIDLLAHIGGFTTKVLAIFGIILATVTTNVAANVV 60
           MG FTFVGVAVTSST +IFG VISNPI+LL  IGG  T +LAI GI LAT+TTN+AANVV
Sbjct: 373 MGLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVV 432

Query: 61  APANALVNLSPSYFTFNRGALLTAVIGILFQPWKLPSSSESFVYTWLVGYSAVLGPIGCV 120
           APANALVNL+P +FTF RGA LTAV+GI+FQPW+L  SSESFVYTWL+GYSA+LGPIG +
Sbjct: 433 APANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGI 492

Query: 121 MVADYHFIRKRELIVEDLYSSSPTGVYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEILS 180
           ++ DY+ I+K +L + DLYS SP+G YY+S G+N+AAV ALV G++PV+PGFL K+  LS
Sbjct: 493 ILVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALS 552

Query: 181 NVPKMFRVIYGNAWFISTFFAGFFFWV 208
            +   F V+Y NA F S   AGF +W+
Sbjct: 553 KISNGFVVVYDNALFFSFIIAGFVYWI 579

The following BLAST results are available for this feature:

BLAST of CmaCh06G004170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZD0	4.5e-72	65.22	Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1	[more]
P94575	5.2e-28	37.25	Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI ...	[more]
Q10279	2.6e-19	33.17	Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812...	[more]
O14035	7.6e-19	35.26	Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / A...	[more]
Q2UPA8	1.2e-16	29.57	Transporter aclS OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN...	[more]

BLAST of CmaCh06G004170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KUM8	1.4e-111	100.00	purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498832 PE=...	[more]
A0A6J1KWR9	3.0e-95	88.83	purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=...	[more]
A0A6J1GDF7	1.7e-93	87.80	purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 P...	[more]
A0A5D3DPI8	1.2e-91	86.41	Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3B756	1.2e-91	86.41	purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1	[more]

BLAST of CmaCh06G004170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023005972.1	2.8e-111	100.00	purine-uracil permease NCS1-like [Cucurbita maxima]	[more]
KAG6596517.1	6.3e-95	88.83	Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023005190.1	6.3e-95	88.83	purine-uracil permease NCS1-like [Cucurbita maxima]	[more]
KAG7028053.1	6.3e-95	88.83	Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_023539092.1	4.1e-94	88.35	purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh06G004170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03555.1	3.2e-73	65.22	permease, cytosine/purines, uracil, thiamine, allantoin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.4160.10	Hydantoin permease	coord: 1..204 e-value: 6.0E-44 score: 152.1
None	No IPR available	PANTHER	PTHR30618	NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER	coord: 1..207
None	No IPR available	PANTHER	PTHR30618:SF0	PURINE-URACIL PERMEASE NCS1	coord: 1..207
IPR001248	Purine-cytosine permease	PFAM	PF02133	Transp_cyt_pur	coord: 3..191 e-value: 1.9E-37 score: 129.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G004170.1	CmaCh06G004170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015851	nucleobase transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0015205	nucleobase transmembrane transporter activity
molecular_function	GO:0019825	oxygen binding
molecular_function	GO:0022857	transmembrane transporter activity