CmaCh06G002960 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G002960 vs. ExPASy Swiss-Prot
Match: F4KCC2 (E3 ubiquitin-protein ligase PRT6 OS=Arabidopsis thaliana OX=3702 GN=PRT6 PE=1 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 1.7e-259
Identity = 491/964 (50.93%), Postives = 649/964 (67.32%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            +++ QRGVCG+VWG NDIAYRCRTCE+DPTCAICVPCFQ+G+H  HDYSII+TGGGCCDC
Sbjct: 113  LNLDQRGVCGSVWGQNDIAYRCRTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDC 172

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GD TAWK +GFCS HKG+EQI+PL E    SV PILD LFTCW +KLLSAE    + ++ 
Sbjct: 173  GDETAWKPDGFCSNHKGSEQIRPLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARS 232

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            +D +   +K++NELTF VVEMLL+F   SESLLSFVS+R+ISS+GLL +L+   R L + 
Sbjct: 233  NDTLVILQKMSNELTFIVVEMLLEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQD 292

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            V+KK+H+L LKL+ +PVFK +FAK F++YYP VISE ++  +D+  KKYPLL TFSVQI 
Sbjct: 293  VMKKLHDLFLKLIGDPVFKCEFAKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQIL 352

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTL P LV+EMNLL++LLGCL DIF+SCS ++G LQ  K   LCET+ RV  D++FVM
Sbjct: 353  TVPTLTPFLVKEMNLLAMLLGCLSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVM 412

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHA+V +Y  +E +++ R+WL LLTF QG++P KRE GI I+EEN+ +HL F L HS+A 
Sbjct: 413  SHAIVSKYATHEHRELSRSWLTLLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAV 472

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVN  +SAAS     ++  A        + +  D D  RYAKVGRLS E + C+ +
Sbjct: 473  IHSLLVNGTYSAASDEEIENDRNAKE------EFDKCDGDGERYAKVGRLSHEDSVCTAI 532

Query: 421  GKSTTSTSASKVDEVRLD----TISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYI 480
              S++  S+   +  ++D     +  + ++L  ECLK +++ L  +    GIS    +++
Sbjct: 533  VSSSSFDSSMASEVHKIDPFHALLPSSAIYLIRECLKVLETCLGNDE---GIS----KFL 592

Query: 481  RMAPSCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSID 540
                S    +  ++    P++    +  G  +   LA  S+D +   S  +   +Q ++ 
Sbjct: 593  CKLSSSSGRNIPESKMSWPRRDLLNVETGGSVSSNLASSSRDPSTGLSP-LCGDIQTNLS 652

Query: 541  IEHGISHGEDNQLMDMTNDTVTVE----DYAMEIDALDYLSLSSWPNIVFDVSSQDIFIH 600
            +++ +         D+T D+  V     D       L  L L  WP+I +DVSSQ I +H
Sbjct: 653  LDN-VCGPYGVVQTDVTADSKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVH 712

Query: 601  IPLHRLLSLLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSI 660
            +PLHRLLSLL+QKALR C+ ESA  N  G S S+      DFF S +        +   +
Sbjct: 713  LPLHRLLSLLIQKALRICYGESASYN--GVSISH-EIPHADFFSSVIGDFHPCGFSALVM 772

Query: 661  LYALKFFVLRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMA 720
             + L+  V    +  G   +     LV      E YRSVRWSEQ LELDLFLLQCCAA+A
Sbjct: 773  EHVLQIRVFCAQVIAGMWKKNGDSALVS----CEWYRSVRWSEQGLELDLFLLQCCAALA 832

Query: 721  PPDFYVSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLK 780
            P D YV ++L RF LS YLSLN +  +EYEP+LV+EML L+IQ++ ERRFCGL+ AESL+
Sbjct: 833  PADSYVDKLLSRFGLSSYLSLNPDITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLR 892

Query: 781  RELIYKLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWK 840
            RE+I+KLA GD THSQLVK+LPRDLSK D+LQE+LD ++VY NPSG NQG YSL    WK
Sbjct: 893  REIIFKLATGDFTHSQLVKSLPRDLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWK 952

Query: 841  ELDLYHPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIV 900
            ELDLYHPRW  +DLQ AEER+ R CGVSALT+QLP+W  IYPP +GL RI TCK   QI+
Sbjct: 953  ELDLYHPRWQSRDLQSAEERFSRYCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQII 1012

Query: 901  RAVLFYSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFA 957
             + L+Y++ +  S KSRAPD  L+TAL LL+L+LDIC  Q++S++      +SIP+L  A
Sbjct: 1013 SSALYYALQSGTSVKSRAPDGVLITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELA 1054

BLAST of CmaCh06G002960 vs. ExPASy Swiss-Prot
Match: Q6WKZ8 (E3 ubiquitin-protein ligase UBR2 OS=Mus musculus OX=10090 GN=Ubr2 PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 2.0e-26
Identity = 221/1043 (21.19%), Postives = 365/1043 (35.00%), Query Frame = 0

Query: 8   VCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHT-GGGCCDCGDVTAW 67
           +CG V+   +  Y CR C  DPTC +C+ CF    H+DH Y +  + GGG CDCGD  AW
Sbjct: 98  LCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAW 157

Query: 68  KREGFCSKHK-----GAEQIQP---LPEEFVKSVEPILDVLF------TCWKSKLLSAEY 127
           K   +C KHK       E+  P   L E+ +     I  ++F        W+ +    E 
Sbjct: 158 KEGPYCQKHKLSSSEVVEEEDPLVHLSEDVIARTYNIFAIMFRYAVDILTWEKESELPED 217

Query: 128 ISVEDSK-------LSDRVTEHKKVANELTFAV----VEML------------------L 187
           + V +          +D V  +++V   L  AV     E +                   
Sbjct: 218 LEVAEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDF 277

Query: 188 DFCKYSESL-------------------------------LSFVSKRVISSAGLLDVL-- 247
            +C  ++++                               LS++   +  S GL  +L  
Sbjct: 278 QYCDQAKTVIVRNTSRQTKPLKVQVMHSSVAAHQNFGLKALSWLGSVIGYSDGLRRILCQ 337

Query: 248 VGLAR-------------LLTESVVKK-----VHELCL-KLLAEPVFKYKFAKVFLNYYP 307
           VGL               +L +S + K      H+L +  LL +  +K  FA  F   Y 
Sbjct: 338 VGLQEGPDGENSSLVDRLMLNDSKLWKGARSVYHQLFMSSLLMDLKYKKLFALRFAKNYR 397

Query: 308 NVISEAIEDNSDHVLKKYPLLPTFSVQIFTVPTLAPRLVEEMNLLSILLGCLEDIFISCS 367
            +  + +ED+ +  +     +   SVQ FT PTLA  L+ E NL+++++    D  +   
Sbjct: 398 QLQRDFMEDDHERAVS----VTALSVQFFTAPTLARMLLTEENLMTVIIKAFMD-HLKHR 457

Query: 368 SKNGRLQVIKWSNLCETTIRVAEDVRFVMSHAVVPRYVIYEQQDILR--------TWLRL 427
              GR Q  +++ L     R  + +   + + ++ +    E  D LR         +L L
Sbjct: 458 DAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPT--EWSDELRQKFLQGFDAFLEL 517

Query: 428 LTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVANIHSLLVNEAFSAASSSSSSSEDT 487
           L  +QG+DP  R+ G HIE E E                                     
Sbjct: 518 LKCMQGMDPITRQVGQHIEMEPE------------------------------------- 577

Query: 488 ADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLLGKSTTSTSASKVDEVRLDTISPT 547
                                          AA +L  K T                   
Sbjct: 578 -----------------------------WEAAFTLQMKLT------------------- 637

Query: 548 IMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAPSCIFYSSRKTSALEPKKMPSKMG 607
                   +  +  W A +                                         
Sbjct: 638 ------HVISMVQDWCALDE---------------------------------------- 697

Query: 608 KGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHGISHGEDNQLMDMTNDTVTVEDYA 667
             K ++E   +                L +      G + GE           +T+    
Sbjct: 698 --KVLIEAYKK---------------CLAVLTQCHGGFTDGE---------QPITLSICG 757

Query: 668 MEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS---LLLQKALRSCFSESAVTNAT 727
             ++ + Y            VS + + IH+P+ RLL+   +LL K      SE A     
Sbjct: 758 HSVETIRYC-----------VSQEKVSIHLPISRLLAGLHVLLSK------SEVAYKFPE 817

Query: 728 GASSSNLSSES-VDFFRSTVIHMVFLHLNLWSILYALKFFVLRLMLERGGGMEMQPYYLV 787
               S LS    ++     ++    +H  +W                 G  +  Q YY  
Sbjct: 818 LLPLSELSPPMLIEHPLRCLVLCAQVHAGMWR--------------RNGFSLVNQIYY-- 877

Query: 788 QPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSRILERFQLSDYLS------- 847
                   Y +V+   +  + D+ +LQ   +M  P+ ++  +L RF+L    S       
Sbjct: 878 --------YHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLSRFELYQLFSTPDYGKR 932

Query: 848 ----LNVESPSEYEPILVKEMLTLIIQVVNERRFCG---LTVAESLKRELIYKLAIGDAT 907
               +  +   +    L++EML LII +V ER   G   +   + +KRE+I++L+I    
Sbjct: 938 FSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQLSIKPMA 932

Query: 908 HSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSIQD 927
           HS+LVK+LP D +K   ++ +++++A +  P    +GMY L  +  KE +LY   +S  +
Sbjct: 998 HSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAE 932

BLAST of CmaCh06G002960 vs. ExPASy Swiss-Prot
Match: Q8IWV8 (E3 ubiquitin-protein ligase UBR2 OS=Homo sapiens OX=9606 GN=UBR2 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.7e-25
Identity = 224/1043 (21.48%), Postives = 365/1043 (35.00%), Query Frame = 0

Query: 8   VCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHT-GGGCCDCGDVTAW 67
           +CG V+   +  Y CR C  DPTC +C+ CF    H+DH Y +  + GGG CDCGD  AW
Sbjct: 98  LCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAW 157

Query: 68  KREGFCSKH--------KGAEQIQPLPEEFVKSVEPILDVLF-------TCWKSKLLSAE 127
           K   +C KH        +  + +  L E+ +     I  + F       T  K   L A+
Sbjct: 158 KEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAITFRYAVEILTWEKESELPAD 217

Query: 128 YISVEDSK------LSDRVTEHKKVANELTFAV----VEML------------------L 187
              VE S        +D V  +++V   L  AV     E +                   
Sbjct: 218 LEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDF 277

Query: 188 DFCKYSES-------------------------------LLSFVSKRVISSAGLLDVL-- 247
            +C+ ++S                               LLS++   +  S GL  +L  
Sbjct: 278 QYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQ 337

Query: 248 VGLAR-------------LLTESVVKK-----VHELCL-KLLAEPVFKYKFAKVFLNYYP 307
           VGL               +L++S + K      H+L +  LL +  +K  FA  F   Y 
Sbjct: 338 VGLQEGPDGENSSLVDRLMLSDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQ 397

Query: 308 NVISEAIEDNSDHVLKKYPLLPTFSVQIFTVPTLAPRLVEEMNLLSILLGCLEDIFISCS 367
            +  + +ED+ +  +     +   SVQ FT PTLA  L+ E NL+SI++    D  +   
Sbjct: 398 QLQRDFMEDDHERAVS----VTALSVQFFTAPTLARMLITEENLMSIIIKTFMD-HLRHR 457

Query: 368 SKNGRLQVIKWSNLCETTIRVAEDVRFVMSHAVVPRYVIYEQQDILR--------TWLRL 427
              GR Q  +++ L     R  + +   + + ++ +    E  D LR         +L L
Sbjct: 458 DAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPT--EWSDELRQKFLEGFDAFLEL 517

Query: 428 LTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVANIHSLLVNEAFSAASSSSSSSEDT 487
           L  +QG+DP  R+ G HIE E E                                     
Sbjct: 518 LKCMQGMDPITRQVGQHIEMEPE------------------------------------- 577

Query: 488 ADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLLGKSTTSTSASKVDEVRLDTISPT 547
                                          AA +L  K T                   
Sbjct: 578 -----------------------------WEAAFTLQMKLT------------------- 637

Query: 548 IMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAPSCIFYSSRKTSALEPKKMPSKMG 607
                   +  +  W A++                                         
Sbjct: 638 ------HVISMMQDWCASDE---------------------------------------- 697

Query: 608 KGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHGISHGEDNQLMDMTNDTVTVEDYA 667
             K ++E   +                L + +    G + GE           +T+    
Sbjct: 698 --KVLIEAYKK---------------CLAVLMQCHGGYTDGE---------QPITLSICG 757

Query: 668 MEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS---LLLQKALRSCFSESAVTNAT 727
             ++ + Y            VS + + IH+P+ RLL+   +LL K      SE A     
Sbjct: 758 HSVETIRYC-----------VSQEKVSIHLPVSRLLAGLHVLLSK------SEVAYKFPE 817

Query: 728 GASSSNLSSES-VDFFRSTVIHMVFLHLNLWSILYALKFFVLRLMLERGGGMEMQPYYLV 787
               S LS    ++     ++    +H  +W                 G  +  Q YY  
Sbjct: 818 LLPLSELSPPMLIEHPLRCLVLCAQVHAGMWR--------------RNGFSLVNQIYY-- 877

Query: 788 QPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSRILERFQLSDYLS------- 847
                   Y +V+   +  + D+ +LQ   +M  P+ ++  +L RF+L    S       
Sbjct: 878 --------YHNVKCRREMFDKDVVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKR 932

Query: 848 ----LNVESPSEYEPILVKEMLTLIIQVVNERRFCG---LTVAESLKRELIYKLAIGDAT 907
               +  +   +    L++EML LII +V ER   G   +   + +KRE+I++L+I    
Sbjct: 938 FSSEITHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVGQVNATDEIKREIIHQLSIKPMA 932

Query: 908 HSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSIQD 927
           HS+LVK+LP D +K   ++ +++ +A +  P    +GMY L  +  KE +LY   +S  +
Sbjct: 998 HSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAE 932

BLAST of CmaCh06G002960 vs. ExPASy Swiss-Prot
Match: Q6ZT12 (E3 ubiquitin-protein ligase UBR3 OS=Homo sapiens OX=9606 GN=UBR3 PE=2 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 8.5e-25
Identity = 209/988 (21.15%), Postives = 377/988 (38.16%), Query Frame = 0

Query: 8   VCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHT-GGGCCDCGDVTAW 67
           +CG VW  N +AYRCRTC   P  ++C  CF  G+H  HD+++  +  GG CDCGD    
Sbjct: 119 LCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVM 178

Query: 68  KREGFCSKH--KGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKLSDRV 127
           +  GFC +H  K +  I  +P++ +   E +L     C    L         D      +
Sbjct: 179 RESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADGPSEKDL 238

Query: 128 TEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTESVVKK 187
            +      +L    +  L D  K   ++ S +++ + +     D+  GL      + VKK
Sbjct: 239 NK----VLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGE---NACVKK 298

Query: 188 VHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIFTVPT 247
            HE  L  L      Y   K+        + E     S           + +VQ F   T
Sbjct: 299 SHEKYLIALKSSGLTYPEDKLVYG-----VQEPSAGTS-----------SLAVQGFIGAT 358

Query: 248 LAPRLV----EEMNLLSILLGCLEDIFISCSSKN-GRLQVIKWSNLCETTIRVAEDVRFV 307
                V    E+    S  LG  + + +S  +K+   + V+K  +  E  +       F 
Sbjct: 359 GTLGQVDSSDEDDQDGSQGLGKRKRVKLSSGTKDQSIMDVLKHKSFLEELLFWTIKYEFP 418

Query: 308 MSHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVA 367
                                 +++TF+  + P +                    ++ VA
Sbjct: 419 Q---------------------KMVTFLLNMLPDQ--------------------EYKVA 478

Query: 368 NIHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSL 427
              + + + AF   +   S   DT   MS +    +     +   A+     Q +  C L
Sbjct: 479 FTKTFVQHYAFIMKTLKKSHESDT---MSNRIVHISVQLFSNEELAR-----QVTEECQL 538

Query: 428 LGKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGI----------S 487
                            LD +   ++++   CL  I S L  E  S  +          +
Sbjct: 539 -----------------LDIMVTVLLYMMESCL--IKSELQDEENSLHVVVNCGEALLKN 598

Query: 488 NMFDEYIRMAPSCIFYSSRKTSALEPK-KMPSKMGKGKFIL-EKLARRSKDHNRQYSSRM 547
           N +   +    + + + S     LE    + + M    F     L +R  + + ++ S+ 
Sbjct: 599 NTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQT 658

Query: 548 Y-----SGLQMSIDIEHG-ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSW---P 607
           Y     + L+       G +SH +  +  + T + V    Y +E       +L  W    
Sbjct: 659 YYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVR---YCLE-------ALQDWFDAI 718

Query: 608 NIVFDVSSQDIFIHIPLHRLLSLLLQKALRSCFSESAVTNATGASSSNLSSESV----DF 667
           N V + +   +  H+PLHR  ++ L KA++                  L  +SV    + 
Sbjct: 719 NFVDEPAPNQVTFHLPLHRYYAMFLSKAVK---------------CQELDLDSVLPDQEM 778

Query: 668 FRSTVIH-------MVFLHLNLWSILYALKFFVLRLMLERGGGMEMQPYYLVQPYYLSEL 727
               +IH       +  +H N+W              +  G  ++ Q    VQ ++ + +
Sbjct: 779 LMKLMIHPLQIQASLAEIHSNMW--------------VRNGLQIKGQAMTYVQSHFCNSM 838

Query: 728 YRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSRILERFQLSDYLSL-----NVESPSEYE 787
                     ++ D++LLQ CA+   PD+++S + ERF++ D L++     N    +E+E
Sbjct: 839 ----------IDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHE 898

Query: 788 PILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLAIGDATHSQLVKALPRD------ 847
             +++  LT ++ +++ R   G++  E L+ E++ +L + D THS L+  +P +      
Sbjct: 899 RSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSG 958

Query: 848 -LSKCDQLQEILDTIAVYSNP-----SGFNQGMYSLHWKYWKELDLYHPRWSI------Q 907
            +      + +L  +A +  P         QGMY+   + W +   + P   I      +
Sbjct: 959 IIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDQ--EFDPVMVILRTVYRR 960

Query: 908 DLQIAEERY---LRSCGVSALTSQLP--KWTKIYPPFRGLTRIATCKTVLQIVRAVLFYS 928
           D+Q A +RY   L+  G        P  K T ++P ++GL R+  CKT L IV   L Y 
Sbjct: 1019 DVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTSLHPSYKGLMRLLHCKT-LHIVLFTLLYK 960

BLAST of CmaCh06G002960 vs. ExPASy Swiss-Prot
Match: Q8IWV7 (E3 ubiquitin-protein ligase UBR1 OS=Homo sapiens OX=9606 GN=UBR1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 2.5e-24
Identity = 214/1036 (20.66%), Postives = 374/1036 (36.10%), Query Frame = 0

Query: 8   VCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSI-IHTGGGCCDCGDVTAW 67
           +CG V+   +  Y CR C  DPTC +C+ CFQ   HK+H Y +   TGGG CDCGD  AW
Sbjct: 98  LCGRVFKSGETTYSCRDCAIDPTCVLCMDCFQDSVHKNHRYKMHTSTGGGFCDCGDTEAW 157

Query: 68  KREGFCSKHK--GAEQIQ-----PLPEEFVKSVEPILD------VLFTCWKSK------- 127
           K   FC  H+   A  I+     PL EE +     I        V  T W+ +       
Sbjct: 158 KTGPFCVNHEPGRAGTIKENSRCPLNEEVIVQARKIFPSVIKYVVEMTIWEEEKELPPEL 217

Query: 128 -----------------------------------------------------LLSAEYI 187
                                                                + +  Y 
Sbjct: 218 QIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAYA 277

Query: 188 SVEDSK-----LSDRVTEHK---KVANELTFAVVEMLLDFCKYSESLLSFVS--KRVISS 247
           + +++K      S+ V++H    +V +    A  +  L    +   ++S+ S  +++   
Sbjct: 278 ACQEAKEDIKSHSENVSQHPLHVEVLHSEIMAHQKFALRLGSWMNKIMSYSSDFRQIFCQ 337

Query: 248 AGLLD-------VLVGLARL----LTESVVKKVHELCL-KLLAEPVFKYKFAKVFLNYYP 307
           A L +        L+    L    L +   K +HEL       E  +K  FA  F+ YY 
Sbjct: 338 ACLREEPDSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYK 397

Query: 308 NVISEAIEDNSDHVLKKYPLLPTFSVQIFTVPTLAPRLVEEMNLLSILLGCLEDIFISCS 367
            +  E I D+ D  +     +   SVQ+FTVPTLA  L+EE N++S++   L ++     
Sbjct: 398 QLQKEYISDDHDRSIS----ITALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYL 457

Query: 368 SKNGRLQVIKWS-NLCETTIRVAEDVRFVMSHAVVPRYVIYEQQ------DILRTWLRLL 427
            +N +     +S +       V  D+++++    + +  I+ ++      +  R++L++L
Sbjct: 458 DRNNKFNFQGYSQDKLGRVYAVICDLKYIL----ISKPTIWTERLRMQFLEGFRSFLKIL 517

Query: 428 TFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVANIHSLLVNEAFSAASSSSSSSEDTA 487
           T +QG++  +R+ G HIE +                                     D  
Sbjct: 518 TCMQGMEEIRRQVGQHIEVD------------------------------------PDWE 577

Query: 488 DAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLLGKSTTSTSASKVDEVRLDTISPTI 547
            A++ Q    N           +  + QE  AC               DE         +
Sbjct: 578 AAIAIQMQLKN-----------ILLMFQEWCAC---------------DE--------EL 637

Query: 548 MWLTY-ECLKTIDSWLATENASGGISNMFDEYIRMAPSCIFYSSRKTSALEPKKMPSKMG 607
           + + Y EC K +                      M  S  F SS KT             
Sbjct: 638 LLVAYKECHKAV----------------------MRCSTSFISSSKTVV----------- 697

Query: 608 KGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHGISHGEDNQLMDMTNDTVTVEDYA 667
                      +S  H+ +  S                                      
Sbjct: 698 -----------QSCGHSLETKS-------------------------------------- 757

Query: 668 MEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLSLLLQKALRSCFSESAVTNATGAS 727
                             + VS   + IH+PL R L+ L  +  R            GA 
Sbjct: 758 ------------------YRVSEDLVSIHLPLSRTLAGLHVRLSR-----------LGAV 817

Query: 728 SSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFVL--RLMLE--RGGGMEMQPYYLV 787
           S      S + F+  V+           + Y L+  VL  +++ E  R  G+ +    + 
Sbjct: 818 SRLHEFVSFEDFQVEVL-----------VEYPLRCLVLVAQVVAEMWRRNGLSL----IS 877

Query: 788 QPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSRILERFQLSDYLSLNVESPS 847
           Q +Y    Y+ V+  E+  + D+ +LQ  A++  P+ ++  +L+R++L++  +  + +  
Sbjct: 878 QVFY----YQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLVLQRYELAEAFNKTISTKD 922

Query: 848 E----YEPILVKEMLTLIIQVVNERRFCG---LTVAESLKRELIYKLAIGDATHSQLVKA 907
           +        L++EML ++I +V ER   G   +T  E   RE+I+ L I    HS + K 
Sbjct: 938 QDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKN 922

Query: 908 LPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPRWSIQDLQIAEER 927
           LP + +    L+ +++ +A +  P     G+Y L  +  K+ ++Y   +S      AE  
Sbjct: 998 LPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHM 922

BLAST of CmaCh06G002960 vs. ExPASy TrEMBL
Match: A0A6J1I3M5 (E3 ubiquitin-protein ligase OS=Cucurbita maxima OX=3661 GN=LOC111470246 PE=3 SV=1)

HSP 1 Score: 1803.9 bits (4671), Expect = 0.0e+00
Identity = 924/968 (95.45%), Postives = 932/968 (96.28%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL
Sbjct: 177  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM
Sbjct: 357  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 477  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP
Sbjct: 537  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG
Sbjct: 597  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS
Sbjct: 657  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHM-VFLHLNLWSILYALKFF 660
            LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRS +     +   +  S+ + L+  
Sbjct: 717  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSVLTDCHPYGFSSSESVEHPLRIK 776

Query: 661  VLRLMLERGGGMEMQPYYLVQPYYL--SELYRSVRWSEQCLELDLFLLQCCAAMAPPDFY 720
            V    +  G         L+    L  SELYRSVRWSEQCLELDLFLLQCCAAMAPPDFY
Sbjct: 777  VFCAEVNAGAWRRNGDAALLSCAALLSSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFY 836

Query: 721  VSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIY 780
            VSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIY
Sbjct: 837  VSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIY 896

Query: 781  KLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLY 840
            KLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLY
Sbjct: 897  KLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLY 956

Query: 841  HPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLF 900
            HPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLF
Sbjct: 957  HPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLF 1016

Query: 901  YSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEID 960
            YSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEID
Sbjct: 1017 YSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEID 1076

Query: 961  EGLTCGFG 966
            EGLTCGFG
Sbjct: 1077 EGLTCGFG 1084

BLAST of CmaCh06G002960 vs. ExPASy TrEMBL
Match: A0A6J1F5I8 (E3 ubiquitin-protein ligase OS=Cucurbita moschata OX=3662 GN=LOC111441022 PE=3 SV=1)

HSP 1 Score: 1751.1 bits (4534), Expect = 0.0e+00
Identity = 893/965 (92.54%), Postives = 911/965 (94.40%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWK+KLLSAEYISVEDSKL
Sbjct: 177  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKNKLLSAEYISVEDSKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SD VTEHKKVANELTFA+VEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGL RLLTES
Sbjct: 237  SDHVTEHKKVANELTFAMVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLERLLTES 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM
Sbjct: 357  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE GIHIEEENENIHLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGIHIEEENENIHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IH+LLVNEAFSAASSSSSSSEDTADAMSFQTYK NPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 477  IHALLVNEAFSAASSSSSSSEDTADAMSFQTYKPNPDDIDSVRYAKVGRLSQESAACSLL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWL TENASGGISNMFDEYIRM P
Sbjct: 537  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLGTENASGGISNMFDEYIRMTP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
             CIFYSSRK SALEPKKM SKMGKGKFILEKLARRSKDHN QYSSRMYSGL MSIDIEHG
Sbjct: 597  ICIFYSSRKASALEPKKMSSKMGKGKFILEKLARRSKDHNSQYSSRMYSGLPMSIDIEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLS+SSWPNIVFDVSSQDI IHIPLHRLLS
Sbjct: 657  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSMSSWPNIVFDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKALRSCFSES VTNATGASSSNLSSESVDFF+S +        + + + + L+  V
Sbjct: 717  LLLQKALRSCFSESVVTNATGASSSNLSSESVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR
Sbjct: 777  FCAEVNAGAWRRNGDAALLS----CELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 836

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA
Sbjct: 837  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 896

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 897  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 956

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQIVRAVLFYSV
Sbjct: 957  WSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQIVRAVLFYSV 1016

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            FTEKSTKSRAPDS LLTALHLLALALDICFMQKESSN SLYARDSIPLLLFAD+EIDEGL
Sbjct: 1017 FTEKSTKSRAPDSVLLTALHLLALALDICFMQKESSNQSLYARDSIPLLLFADEEIDEGL 1076

Query: 961  TCGFG 966
            TCGFG
Sbjct: 1077 TCGFG 1077

BLAST of CmaCh06G002960 vs. ExPASy TrEMBL
Match: A0A1S3B5X9 (E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103486385 PE=3 SV=1)

HSP 1 Score: 1562.0 bits (4043), Expect = 0.0e+00
Identity = 804/965 (83.32%), Postives = 852/965 (88.29%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSII+TGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GD TAWKREGFCSKHKGAEQIQPLPEE+VKSV PILD LFT WK+KLLSAE ISVED KL
Sbjct: 177  GDATAWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAGLLD+LV L RLLT+S
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDS 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYP VISEAIED+SDH LKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTL PRLVEEMNLLSILLGCLEDIFISC S++GRLQV+KWSNL ETTIRV EDVRFVM
Sbjct: 357  TVPTLTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE G+HIEEENEN+HLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLV EAFSAA   SSS EDTAD M FQTYK N DDIDSVR+AKVGRLSQESAAC +L
Sbjct: 477  IHSLLVKEAFSAA---SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKS+TSTSAS+VDEV  D IS TIMWLTYECLK ID+WL TEN SG I NM  E I  AP
Sbjct: 537  GKSSTSTSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SC FYSSRKTSAL  KKM  KM KGKFI +KL+RRSK HNRQYSSRMYSGLQMSID EHG
Sbjct: 597  SCKFYSSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            IS GEDN LMD+TNDTVT EDYAMEIDAL +LSLSSWPNIV+DVSSQDI IHIPLHRLLS
Sbjct: 657  ISLGEDNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKAL SCFSES V +ATGASSSNLSSE VDFF+S +        + + + + L+  V
Sbjct: 717  LLLQKALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRS+RWSEQCLELDLFLLQCCAAMAPPD YV R
Sbjct: 777  FCAEVNAGMWRRNGDAALLS----CELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCR 836

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERF+LSDYLSLNVE PSEYEPILV+EMLTLIIQVVNERRFCGLT+AESLKRELIYKLA
Sbjct: 837  ILERFRLSDYLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLA 896

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKC QLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 897  IGDATHSQLVKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 956

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQ+AEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQ +RAVLFYSV
Sbjct: 957  WSLRDLQVAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSV 1016

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            F E STKSRAPDS LL+ALHLLALALD+CF QKES + S  A DSIPLLLFA +EI+EGL
Sbjct: 1017 FAEISTKSRAPDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGL 1074

Query: 961  TCGFG 966
              GFG
Sbjct: 1077 AYGFG 1074

BLAST of CmaCh06G002960 vs. ExPASy TrEMBL
Match: A0A5D3DN55 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001660 PE=3 SV=1)

HSP 1 Score: 1562.0 bits (4043), Expect = 0.0e+00
Identity = 804/965 (83.32%), Postives = 852/965 (88.29%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSII+TGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GD TAWKREGFCSKHKGAEQIQPLPEE+VKSV PILD LFT WK+KLLSAE ISVED KL
Sbjct: 177  GDATAWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAGLLD+LV L RLLT+S
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDS 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYP VISEAIED+SDH LKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTL PRLVEEMNLLSILLGCLEDIFISC S++GRLQV+KWSNL ETTIRV EDVRFVM
Sbjct: 357  TVPTLTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE G+HIEEENEN+HLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLV EAFSAA   SSS EDTAD M FQTYK N DDIDSVR+AKVGRLSQESAAC +L
Sbjct: 477  IHSLLVKEAFSAA---SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKS+TSTSAS+VDEV  D IS TIMWLTYECLK ID+WL TEN SG I NM  E I  AP
Sbjct: 537  GKSSTSTSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SC FYSSRKTSAL  KKM  KM KGKFI +KL+RRSK HNRQYSSRMYSGLQMSID EHG
Sbjct: 597  SCKFYSSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            IS GEDN LMD+TNDTVT EDYAMEIDAL +LSLSSWPNIV+DVSSQDI IHIPLHRLLS
Sbjct: 657  ISLGEDNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKAL SCFSES V +ATGASSSNLSSE VDFF+S +        + + + + L+  V
Sbjct: 717  LLLQKALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRS+RWSEQCLELDLFLLQCCAAMAPPD YV R
Sbjct: 777  FCAEVNAGMWRRNGDAALLS----CELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCR 836

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERF+LSDYLSLNVE PSEYEPILV+EMLTLIIQVVNERRFCGLT+AESLKRELIYKLA
Sbjct: 837  ILERFRLSDYLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLA 896

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKC QLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 897  IGDATHSQLVKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 956

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQ+AEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQ +RAVLFYSV
Sbjct: 957  WSLRDLQVAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSV 1016

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            F E STKSRAPDS LL+ALHLLALALD+CF QKES + S  A DSIPLLLFA +EI+EGL
Sbjct: 1017 FAEISTKSRAPDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGL 1074

Query: 961  TCGFG 966
              GFG
Sbjct: 1077 AYGFG 1074

BLAST of CmaCh06G002960 vs. ExPASy TrEMBL
Match: A0A5A7TL84 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004420 PE=3 SV=1)

HSP 1 Score: 1562.0 bits (4043), Expect = 0.0e+00
Identity = 804/965 (83.32%), Postives = 852/965 (88.29%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNH+DHDYSII+TGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHQDHDYSIIYTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GD TAWKREGFCSKHKGAEQIQPLPEE+VKSV PILD LFT WK+KLLSAE ISVED KL
Sbjct: 177  GDATAWKREGFCSKHKGAEQIQPLPEEYVKSVGPILDALFTSWKNKLLSAEDISVEDPKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCK+SESLLSFVSKRVISSAGLLD+LV L RLLT+S
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKHSESLLSFVSKRVISSAGLLDILVRLERLLTDS 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYP VISEAIED+SDH LKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPTVISEAIEDSSDHALKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTL PRLVEEMNLLSILLGCLEDIFISC S++GRLQV+KWSNL ETTIRV EDVRFVM
Sbjct: 357  TVPTLTPRLVEEMNLLSILLGCLEDIFISCVSEDGRLQVVKWSNLYETTIRVVEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE G+HIEEENEN+HLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGLHIEEENENVHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLV EAFSAA   SSS EDTAD M FQTYK N DDIDSVR+AKVGRLSQESAAC +L
Sbjct: 477  IHSLLVKEAFSAA---SSSCEDTADGMYFQTYKQNVDDIDSVRHAKVGRLSQESAACGVL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKS+TSTSAS+VDEV  D IS TIMWLTYECLK ID+WL TEN SG I NM  E I  AP
Sbjct: 537  GKSSTSTSASRVDEVCSDAISSTIMWLTYECLKIIDNWLGTENISGSIPNMLGESISPAP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SC FYSSRKTSAL  KKM  KM KGKFI +KL+RRSK HNRQYSSRMYSGLQMSID EHG
Sbjct: 597  SCKFYSSRKTSALASKKMSYKMEKGKFIFDKLSRRSKYHNRQYSSRMYSGLQMSIDNEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            IS GEDN LMD+TNDTVT EDYAMEIDAL +LSLSSWPNIV+DVSSQDI IHIPLHRLLS
Sbjct: 657  ISLGEDNHLMDVTNDTVTDEDYAMEIDALHFLSLSSWPNIVYDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKAL SCFSES V +ATGASSSNLSSE VDFF+S +        + + + + L+  V
Sbjct: 717  LLLQKALSSCFSESGVPSATGASSSNLSSEYVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRS+RWSEQCLELDLFLLQCCAAMAPPD YV R
Sbjct: 777  FCAEVNAGMWRRNGDAALLS----CELYRSIRWSEQCLELDLFLLQCCAAMAPPDLYVCR 836

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERF+LSDYLSLNVE PSEYEPILV+EMLTLIIQVVNERRFCGLT+AESLKRELIYKLA
Sbjct: 837  ILERFRLSDYLSLNVERPSEYEPILVQEMLTLIIQVVNERRFCGLTIAESLKRELIYKLA 896

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKC QLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 897  IGDATHSQLVKALPRDLSKCHQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 956

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQ+AEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQ +RAVLFYSV
Sbjct: 957  WSLRDLQVAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQFIRAVLFYSV 1016

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            F E STKSRAPDS LL+ALHLLALALD+CF QKES + S  A DSIPLLLFA +EI+EGL
Sbjct: 1017 FAEISTKSRAPDSVLLSALHLLALALDVCFQQKESRDQSFDAPDSIPLLLFAAEEIEEGL 1074

Query: 961  TCGFG 966
              GFG
Sbjct: 1077 AYGFG 1074

BLAST of CmaCh06G002960 vs. NCBI nr
Match: XP_022971566.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase PRT6-like [Cucurbita maxima])

HSP 1 Score: 1803.9 bits (4671), Expect = 0.0e+00
Identity = 924/968 (95.45%), Postives = 932/968 (96.28%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL
Sbjct: 177  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM
Sbjct: 357  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 477  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP
Sbjct: 537  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG
Sbjct: 597  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS
Sbjct: 657  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHM-VFLHLNLWSILYALKFF 660
            LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRS +     +   +  S+ + L+  
Sbjct: 717  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSVLTDCHPYGFSSSESVEHPLRIK 776

Query: 661  VLRLMLERGGGMEMQPYYLVQPYYL--SELYRSVRWSEQCLELDLFLLQCCAAMAPPDFY 720
            V    +  G         L+    L  SELYRSVRWSEQCLELDLFLLQCCAAMAPPDFY
Sbjct: 777  VFCAEVNAGAWRRNGDAALLSCAALLSSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFY 836

Query: 721  VSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIY 780
            VSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIY
Sbjct: 837  VSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIY 896

Query: 781  KLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLY 840
            KLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLY
Sbjct: 897  KLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLY 956

Query: 841  HPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLF 900
            HPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLF
Sbjct: 957  HPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLF 1016

Query: 901  YSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEID 960
            YSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEID
Sbjct: 1017 YSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEID 1076

Query: 961  EGLTCGFG 966
            EGLTCGFG
Sbjct: 1077 EGLTCGFG 1084

BLAST of CmaCh06G002960 vs. NCBI nr
Match: KAG6596407.1 (E3 ubiquitin-protein ligase PRT6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1751.9 bits (4536), Expect = 0.0e+00
Identity = 893/965 (92.54%), Postives = 911/965 (94.40%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 356  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 415

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWK+KL+SAEYISVED KL
Sbjct: 416  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKNKLVSAEYISVEDCKL 475

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGL RLLTES
Sbjct: 476  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLERLLTES 535

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 536  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 595

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQV+KWSNLCETTIRVAEDVRFVM
Sbjct: 596  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVLKWSNLCETTIRVAEDVRFVM 655

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE GIHIEEENENIHLPFGLDHSVAN
Sbjct: 656  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGIHIEEENENIHLPFGLDHSVAN 715

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYK NPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 716  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKPNPDDIDSVRYAKVGRLSQESAACSLL 775

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWL TENASGGISNMFDEYIRM P
Sbjct: 776  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLGTENASGGISNMFDEYIRMTP 835

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SCIFYSSRK SALEPKKM  KMGKGKFILEKLARRSKDHN QYSSRMYSGL MSIDIEHG
Sbjct: 836  SCIFYSSRKASALEPKKMSPKMGKGKFILEKLARRSKDHNSQYSSRMYSGLPMSIDIEHG 895

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLS+SSWPNIVFDVSSQDI IHIPLHRLLS
Sbjct: 896  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSMSSWPNIVFDVSSQDISIHIPLHRLLS 955

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKALRSCFSES VTNATGASSSNLSSESVDFF+S +        + + + + L+  V
Sbjct: 956  LLLQKALRSCFSESVVTNATGASSSNLSSESVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 1015

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR
Sbjct: 1016 FCAEVNAGAWRRNGDAALLS----CELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 1075

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA
Sbjct: 1076 ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 1135

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 1136 IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 1195

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQIVRAVLFYSV
Sbjct: 1196 WSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQIVRAVLFYSV 1255

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            FTEKSTKSRAPDS LLTALHLLALALDICFMQKESSN SLYARDSIPLLLFAD+EIDEGL
Sbjct: 1256 FTEKSTKSRAPDSVLLTALHLLALALDICFMQKESSNQSLYARDSIPLLLFADEEIDEGL 1315

Query: 961  TCGFG 966
            TCGFG
Sbjct: 1316 TCGFG 1316

BLAST of CmaCh06G002960 vs. NCBI nr
Match: XP_022933688.1 (E3 ubiquitin-protein ligase PRT6-like [Cucurbita moschata])

HSP 1 Score: 1751.1 bits (4534), Expect = 0.0e+00
Identity = 893/965 (92.54%), Postives = 911/965 (94.40%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWK+KLLSAEYISVEDSKL
Sbjct: 177  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKNKLLSAEYISVEDSKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SD VTEHKKVANELTFA+VEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGL RLLTES
Sbjct: 237  SDHVTEHKKVANELTFAMVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLERLLTES 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM
Sbjct: 357  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE GIHIEEENENIHLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGIHIEEENENIHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IH+LLVNEAFSAASSSSSSSEDTADAMSFQTYK NPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 477  IHALLVNEAFSAASSSSSSSEDTADAMSFQTYKPNPDDIDSVRYAKVGRLSQESAACSLL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWL TENASGGISNMFDEYIRM P
Sbjct: 537  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLGTENASGGISNMFDEYIRMTP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
             CIFYSSRK SALEPKKM SKMGKGKFILEKLARRSKDHN QYSSRMYSGL MSIDIEHG
Sbjct: 597  ICIFYSSRKASALEPKKMSSKMGKGKFILEKLARRSKDHNSQYSSRMYSGLPMSIDIEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLS+SSWPNIVFDVSSQDI IHIPLHRLLS
Sbjct: 657  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSMSSWPNIVFDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKALRSCFSES VTNATGASSSNLSSESVDFF+S +        + + + + L+  V
Sbjct: 717  LLLQKALRSCFSESVVTNATGASSSNLSSESVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR
Sbjct: 777  FCAEVNAGAWRRNGDAALLS----CELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 836

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA
Sbjct: 837  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 896

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 897  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 956

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQIVRAVLFYSV
Sbjct: 957  WSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQIVRAVLFYSV 1016

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            FTEKSTKSRAPDS LLTALHLLALALDICFMQKESSN SLYARDSIPLLLFAD+EIDEGL
Sbjct: 1017 FTEKSTKSRAPDSVLLTALHLLALALDICFMQKESSNQSLYARDSIPLLLFADEEIDEGL 1076

Query: 961  TCGFG 966
            TCGFG
Sbjct: 1077 TCGFG 1077

BLAST of CmaCh06G002960 vs. NCBI nr
Match: XP_023539127.1 (E3 ubiquitin-protein ligase PRT6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1738.8 bits (4502), Expect = 0.0e+00
Identity = 892/965 (92.44%), Postives = 908/965 (94.09%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWK+KLLSAEYISVEDSKL
Sbjct: 177  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKNKLLSAEYISVEDSKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGL RLLTES
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLERLLTES 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EP FKY+FAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPAFKYEFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM
Sbjct: 357  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE GIHIEEENENIHLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGIHIEEENENIHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYK NPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 477  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKPNPDDIDSVRYAKVGRLSQESAACSLL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTI  LTYECLKTIDSWL TENASGGISNMFDEYIRMAP
Sbjct: 537  GKSTTSTSASKVDEVRLDTISPTITRLTYECLKTIDSWLGTENASGGISNMFDEYIRMAP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SCIFYSSRKTSALEPKKM SKMGKG     KLARRSKDHN QYSSRMYSGLQMSIDIEHG
Sbjct: 597  SCIFYSSRKTSALEPKKMSSKMGKG-----KLARRSKDHNSQYSSRMYSGLQMSIDIEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLS+SSWPNIVFDVSSQDI IHIPLHRLLS
Sbjct: 657  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSMSSWPNIVFDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKALRSCFSES VTNATGASSSNLSSESVDFFRS +        + + + + L+  V
Sbjct: 717  LLLQKALRSCFSESVVTNATGASSSNLSSESVDFFRSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRSVRWSEQCLELDLFLLQCCAAM+PPDFYVSR
Sbjct: 777  FCAEVNAGAWRRNGDAALLS----CELYRSVRWSEQCLELDLFLLQCCAAMSPPDFYVSR 836

Query: 721  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 780
            ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA
Sbjct: 837  ILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLKRELIYKLA 896

Query: 781  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 840
            IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR
Sbjct: 897  IGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWKELDLYHPR 956

Query: 841  WSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIVRAVLFYSV 900
            WS++DLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGL RIATCKTVLQIVRAVLFYSV
Sbjct: 957  WSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARIATCKTVLQIVRAVLFYSV 1016

Query: 901  FTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFADKEIDEGL 960
            FTEKSTKSRAPDS LLTALHLLALALDICFMQKESSN SLYARDSIPLLLFAD+EIDEGL
Sbjct: 1017 FTEKSTKSRAPDSVLLTALHLLALALDICFMQKESSNQSLYARDSIPLLLFADEEIDEGL 1072

Query: 961  TCGFG 966
            TCGFG
Sbjct: 1077 TCGFG 1072

BLAST of CmaCh06G002960 vs. NCBI nr
Match: KAG7027950.1 (E3 ubiquitin-protein ligase PRT6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1737.2 bits (4498), Expect = 0.0e+00
Identity = 891/983 (90.64%), Postives = 909/983 (92.47%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC
Sbjct: 117  MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 176

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWK+KL+SAEYISVED KL
Sbjct: 177  GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKNKLVSAEYISVEDCKL 236

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGL RLLTES
Sbjct: 237  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLERLLTES 296

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            VVKKVHEL LKLL EPVFKY+FAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF
Sbjct: 297  VVKKVHELLLKLLGEPVFKYEFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 356

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQV+KWSNLCETTIRVAEDVRFVM
Sbjct: 357  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVLKWSNLCETTIRVAEDVRFVM 416

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHAVVPRYVIYEQQDILRTWLRLLTFVQG+DPQKRE GIHIEEENENIHLPFGLDHSVAN
Sbjct: 417  SHAVVPRYVIYEQQDILRTWLRLLTFVQGMDPQKRETGIHIEEENENIHLPFGLDHSVAN 476

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVNEAFSAASSSS SSEDTADAMSFQTYK NPDDIDSVRYAKVGRLSQESAACSLL
Sbjct: 477  IHSLLVNEAFSAASSSSRSSEDTADAMSFQTYKPNPDDIDSVRYAKVGRLSQESAACSLL 536

Query: 421  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYIRMAP 480
            GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWL TENASGGISNMFDEYIRM P
Sbjct: 537  GKSTTSTSASKVDEVRLDTISPTIMWLTYECLKTIDSWLGTENASGGISNMFDEYIRMTP 596

Query: 481  SCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSIDIEHG 540
            SCIFYSSRK SALEPKKM  KMGKGKFILEKLARRSKDHN QYSSRMYSGL MSIDIEHG
Sbjct: 597  SCIFYSSRKASALEPKKMSPKMGKGKFILEKLARRSKDHNSQYSSRMYSGLPMSIDIEHG 656

Query: 541  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSLSSWPNIVFDVSSQDIFIHIPLHRLLS 600
            ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLS+SSWPNIVFDVSSQDI IHIPLHRLLS
Sbjct: 657  ISHGEDNQLMDMTNDTVTVEDYAMEIDALDYLSMSSWPNIVFDVSSQDISIHIPLHRLLS 716

Query: 601  LLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSILYALKFFV 660
            LLLQKALR CFSES VTNATGASSSNLSSESVDFF+S +        + + + + L+  V
Sbjct: 717  LLLQKALRGCFSESVVTNATGASSSNLSSESVDFFKSVLTDCHPYGFSSFVMEHPLRIKV 776

Query: 661  LRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 720
                +  G         L+      ELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR
Sbjct: 777  FCAEVNAGAWRRNGDAALLS----CELYRSVRWSEQCLELDLFLLQCCAAMAPPDFYVSR 836

Query: 721  ILERFQLSDYLSLNVESPSE------------------YEPILVKEMLTLIIQVVNERRF 780
            ILERFQLSDYLSLNVESPSE                  YEPILVKEMLTLIIQVVNERRF
Sbjct: 837  ILERFQLSDYLSLNVESPSEYVLIYYFDLERYCSSKIRYEPILVKEMLTLIIQVVNERRF 896

Query: 781  CGLTVAESLKRELIYKLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQG 840
            CGLTVAESLKRELIYKLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQG
Sbjct: 897  CGLTVAESLKRELIYKLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQG 956

Query: 841  MYSLHWKYWKELDLYHPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRI 900
            MYSLHWKYWKELDLYHPRWS++DLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGL RI
Sbjct: 957  MYSLHWKYWKELDLYHPRWSLRDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLARI 1016

Query: 901  ATCKTVLQIVRAVLFYSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYA 960
            ATCKTVLQIVRAVLFYSVFTEKSTKSRAPDS LLTALHLLALALDICFMQKESSN SLYA
Sbjct: 1017 ATCKTVLQIVRAVLFYSVFTEKSTKSRAPDSVLLTALHLLALALDICFMQKESSNQSLYA 1076

Query: 961  RDSIPLLLFADKEIDEGLTCGFG 966
            RDSIPLLLFAD+EIDEGLTCGFG
Sbjct: 1077 RDSIPLLLFADEEIDEGLTCGFG 1095

BLAST of CmaCh06G002960 vs. TAIR 10
Match: AT5G02310.1 (proteolysis 6 )

HSP 1 Score: 897.1 bits (2317), Expect = 1.2e-260
Identity = 491/964 (50.93%), Postives = 649/964 (67.32%), Query Frame = 0

Query: 1    MSVGQRGVCGAVWGHNDIAYRCRTCEHDPTCAICVPCFQSGNHKDHDYSIIHTGGGCCDC 60
            +++ QRGVCG+VWG NDIAYRCRTCE+DPTCAICVPCFQ+G+H  HDYSII+TGGGCCDC
Sbjct: 113  LNLDQRGVCGSVWGQNDIAYRCRTCENDPTCAICVPCFQNGDHNSHDYSIIYTGGGCCDC 172

Query: 61   GDVTAWKREGFCSKHKGAEQIQPLPEEFVKSVEPILDVLFTCWKSKLLSAEYISVEDSKL 120
            GD TAWK +GFCS HKG+EQI+PL E    SV PILD LFTCW +KLLSAE    + ++ 
Sbjct: 173  GDETAWKPDGFCSNHKGSEQIRPLSENLANSVGPILDALFTCWNNKLLSAESSGQKGARS 232

Query: 121  SDRVTEHKKVANELTFAVVEMLLDFCKYSESLLSFVSKRVISSAGLLDVLVGLARLLTES 180
            +D +   +K++NELTF VVEMLL+F   SESLLSFVS+R+ISS+GLL +L+   R L + 
Sbjct: 233  NDTLVILQKMSNELTFIVVEMLLEFSMSSESLLSFVSRRIISSSGLLSILLKAERFLDQD 292

Query: 181  VVKKVHELCLKLLAEPVFKYKFAKVFLNYYPNVISEAIEDNSDHVLKKYPLLPTFSVQIF 240
            V+KK+H+L LKL+ +PVFK +FAK F++YYP VISE ++  +D+  KKYPLL TFSVQI 
Sbjct: 293  VMKKLHDLFLKLIGDPVFKCEFAKAFVSYYPVVISEVVKQGTDNAFKKYPLLSTFSVQIL 352

Query: 241  TVPTLAPRLVEEMNLLSILLGCLEDIFISCSSKNGRLQVIKWSNLCETTIRVAEDVRFVM 300
            TVPTL P LV+EMNLL++LLGCL DIF+SCS ++G LQ  K   LCET+ RV  D++FVM
Sbjct: 353  TVPTLTPFLVKEMNLLAMLLGCLSDIFVSCSGEDGLLQATKLERLCETSERVIGDLKFVM 412

Query: 301  SHAVVPRYVIYEQQDILRTWLRLLTFVQGVDPQKREAGIHIEEENENIHLPFGLDHSVAN 360
            SHA+V +Y  +E +++ R+WL LLTF QG++P KRE GI I+EEN+ +HL F L HS+A 
Sbjct: 413  SHAIVSKYATHEHRELSRSWLTLLTFAQGMNPLKRETGIPIDEENDYMHLFFVLGHSIAV 472

Query: 361  IHSLLVNEAFSAASSSSSSSEDTADAMSFQTYKTNPDDIDSVRYAKVGRLSQESAACSLL 420
            IHSLLVN  +SAAS     ++  A        + +  D D  RYAKVGRLS E + C+ +
Sbjct: 473  IHSLLVNGTYSAASDEEIENDRNAKE------EFDKCDGDGERYAKVGRLSHEDSVCTAI 532

Query: 421  GKSTTSTSASKVDEVRLD----TISPTIMWLTYECLKTIDSWLATENASGGISNMFDEYI 480
              S++  S+   +  ++D     +  + ++L  ECLK +++ L  +    GIS    +++
Sbjct: 533  VSSSSFDSSMASEVHKIDPFHALLPSSAIYLIRECLKVLETCLGNDE---GIS----KFL 592

Query: 481  RMAPSCIFYSSRKTSALEPKKMPSKMGKGKFILEKLARRSKDHNRQYSSRMYSGLQMSID 540
                S    +  ++    P++    +  G  +   LA  S+D +   S  +   +Q ++ 
Sbjct: 593  CKLSSSSGRNIPESKMSWPRRDLLNVETGGSVSSNLASSSRDPSTGLSP-LCGDIQTNLS 652

Query: 541  IEHGISHGEDNQLMDMTNDTVTVE----DYAMEIDALDYLSLSSWPNIVFDVSSQDIFIH 600
            +++ +         D+T D+  V     D       L  L L  WP+I +DVSSQ I +H
Sbjct: 653  LDN-VCGPYGVVQTDVTADSKRVSCNSADLTKNASGLRILGLCDWPDIHYDVSSQAISVH 712

Query: 601  IPLHRLLSLLLQKALRSCFSESAVTNATGASSSNLSSESVDFFRSTVIHMVFLHLNLWSI 660
            +PLHRLLSLL+QKALR C+ ESA  N  G S S+      DFF S +        +   +
Sbjct: 713  LPLHRLLSLLIQKALRICYGESASYN--GVSISH-EIPHADFFSSVIGDFHPCGFSALVM 772

Query: 661  LYALKFFVLRLMLERGGGMEMQPYYLVQPYYLSELYRSVRWSEQCLELDLFLLQCCAAMA 720
             + L+  V    +  G   +     LV      E YRSVRWSEQ LELDLFLLQCCAA+A
Sbjct: 773  EHVLQIRVFCAQVIAGMWKKNGDSALVS----CEWYRSVRWSEQGLELDLFLLQCCAALA 832

Query: 721  PPDFYVSRILERFQLSDYLSLNVESPSEYEPILVKEMLTLIIQVVNERRFCGLTVAESLK 780
            P D YV ++L RF LS YLSLN +  +EYEP+LV+EML L+IQ++ ERRFCGL+ AESL+
Sbjct: 833  PADSYVDKLLSRFGLSSYLSLNPDITNEYEPVLVQEMLGLLIQILQERRFCGLSTAESLR 892

Query: 781  RELIYKLAIGDATHSQLVKALPRDLSKCDQLQEILDTIAVYSNPSGFNQGMYSLHWKYWK 840
            RE+I+KLA GD THSQLVK+LPRDLSK D+LQE+LD ++VY NPSG NQG YSL    WK
Sbjct: 893  REIIFKLATGDFTHSQLVKSLPRDLSKSDELQEVLDDVSVYCNPSGMNQGKYSLQSSCWK 952

Query: 841  ELDLYHPRWSIQDLQIAEERYLRSCGVSALTSQLPKWTKIYPPFRGLTRIATCKTVLQIV 900
            ELDLYHPRW  +DLQ AEER+ R CGVSALT+QLP+W  IYPP +GL RI TCK   QI+
Sbjct: 953  ELDLYHPRWQSRDLQSAEERFSRYCGVSALTTQLPRWRMIYPPLKGLARIGTCKATFQII 1012

Query: 901  RAVLFYSVFTEKSTKSRAPDSGLLTALHLLALALDICFMQKESSNPSLYARDSIPLLLFA 957
             + L+Y++ +  S KSRAPD  L+TAL LL+L+LDIC  Q++S++      +SIP+L  A
Sbjct: 1013 SSALYYALQSGTSVKSRAPDGVLITALQLLSLSLDICTQQRQSNSQDCCLENSIPILELA 1054