CmaCh06G001110 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G001110
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	3-methyladenine DNA glycosidase
Location	Cma_Chr06: 595579 .. 596239 (+)
RNA-Seq Expression	CmaCh06G001110
Synteny	CmaCh06G001110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCCAAATTGGACACCATTGGGGCCAAATCGAAACCCGATATCAGCCCACAAATCCAAATTGCTCTATTCTAACCCTCTTCCTCTTTCGAGTGATTCACTAAAGCTCCCCGAAGAGTTGTTCCGCGTCGACGCTTGGGGCCTTGCCCCACGCTTGCTTGGAAAGCTCCTCCGGAGAGACGGTCTTGTTCTTCGGATCACTGAGGTGCCATTCCTCGTTCTTTGTTATCACCATATGATTTGGGTTGCATTTAGCTTGTTAATGGGGACGAGAAGCTCCTGTTTGCTTATTTGTTTGCATGCCAAGTCTCTGGGAGACATGCCCACAACCTGTTTGAATGTTTGTCTGTGTGAATATCAAGTTACCGAAATCTACTTCTCCACAAACGGGAGCTGAATTTTTATGGTGGTACTGTGATTATATCTTGCATTTGTGCACAAAAGTCCAAAATAGTTGTTCACTTCTTTATACCGACCATTGATAAAAAAGATTGTTGATAGGTAGAAGCTTACAGACAGAATGATACTGCCTGTCATGGTCGGTTTGGTGTCACAGCAAGAACGACTCCAGAAGTAAGTTCTTCTTAACTGATTATCGACGGGAAAATGAAGAATTGTATATCTAATTCTTATAGAGTCTTTATTCATCTTAGACATAG

mRNA sequence

ATGGCCCCAAATTGGACACCATTGGGGCCAAATCGAAACCCGATATCAGCCCACAAATCCAAATTGCTCTATTCTAACCCTCTTCCTCTTTCGAGTGATTCACTAAAGCTCCCCGAAGAGTTGTTCCGCGTCGACGCTTGGGGCCTTGCCCCACGCTTGCTTGGAAAGCTCCTCCGGAGAGACGGTCTTGTTCTTCGGATCACTGAGGTAGAAGCTTACAGACAGAATGATACTGCCTGTCATGGTCGGTTTGGTGTCACAGCAAGAACGACTCCAGAAACATAG

Coding sequence (CDS)

ATGGCCCCAAATTGGACACCATTGGGGCCAAATCGAAACCCGATATCAGCCCACAAATCCAAATTGCTCTATTCTAACCCTCTTCCTCTTTCGAGTGATTCACTAAAGCTCCCCGAAGAGTTGTTCCGCGTCGACGCTTGGGGCCTTGCCCCACGCTTGCTTGGAAAGCTCCTCCGGAGAGACGGTCTTGTTCTTCGGATCACTGAGGTAGAAGCTTACAGACAGAATGATACTGCCTGTCATGGTCGGTTTGGTGTCACAGCAAGAACGACTCCAGAAACATAG

Protein sequence

MAPNWTPLGPNRNPISAHKSKLLYSNPLPLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACHGRFGVTARTTPET

Homology

[Close]

BLAST of CmaCh06G001110 vs. ExPASy Swiss-Prot
Match: Q39147 (DNA-3-methyladenine glycosylase OS=Arabidopsis thaliana OX=3702 GN=MAG PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.4e-18
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 32  SDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACHGRFGVTARTT 91
           S+   +P E F++DA  LAPRLLGK +RRD +VLRITEVEAYR ND+ACHGRFGVT RT 
Sbjct: 54  SEMKLMPPEFFQIDALDLAPRLLGKFMRRDNVVLRITEVEAYRPNDSACHGRFGVTPRTA 113

Query: 92  P 93
           P
Sbjct: 114 P 114

[Close]

BLAST of CmaCh06G001110 vs. ExPASy Swiss-Prot
Match: Q3JEY0 (Putative 3-methyladenine DNA glycosylase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=Noc_0079 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.9e-06
Identity = 31/57 (54.39%), Postives = 35/57 (61.40%), Query Frame = 0


Query: 37 LPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYR-QNDTACHGRFGVTARTTP 93
          LP   +  DA  +A  LLG  L R+ +VLRITEVEAYR   DTA HGR G T R  P
Sbjct: 5  LPPRFYARDALEVAADLLGASLCREQVVLRITEVEAYRWPEDTANHGRHGQTLRNEP 61

[Close]

BLAST of CmaCh06G001110 vs. ExPASy TrEMBL
Match: A0A6J1J112 (3-methyladenine DNA glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111481712 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.9e-18
Identity = 50/79 (63.29%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 14  PISAHKSKLLYSNPLPLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAY 73
           P++  ++++  S+P   S     LP+E F VDA  LAPRLLGKLLRRD +VLRITEVEAY
Sbjct: 78  PVTDPQTQIAQSSPSSSSEGFTILPKEFFLVDALDLAPRLLGKLLRRDDVVLRITEVEAY 137

Query: 74  RQNDTACHGRFGVTARTTP 93
           R ND+ACHGRFGVTART P
Sbjct: 138 RPNDSACHGRFGVTARTAP 156

[Close]

BLAST of CmaCh06G001110 vs. ExPASy TrEMBL
Match: A0A5J9UZT0 (3-methyladenine DNA glycosidase (Fragment) OS=Eragrostis curvula OX=38414 GN=EJB05_20286 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.9e-18
Identity = 51/71 (71.83%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 27  PLPLSSDSL-----KLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACH 86
           P PL SD+L      LP+E F VDA  LAPRLLGKLLRRD +VLRITEVEAYR ND+ACH
Sbjct: 99  PAPLVSDALPHIGRALPQEFFEVDALDLAPRLLGKLLRRDEVVLRITEVEAYRPNDSACH 158

Query: 87  GRFGVTARTTP 93
           GRFG+TART P
Sbjct: 159 GRFGITARTAP 169

[Close]

BLAST of CmaCh06G001110 vs. ExPASy TrEMBL
Match: A0A6A6KNC4 (3-methyladenine DNA glycosidase OS=Hevea brasiliensis OX=3981 GN=GH714_036770 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.1e-17
Identity = 49/72 (68.06%), Postives = 57/72 (79.17%), Query Frame = 0


Query: 22  LLYSNPLPLSSDSLK-LPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTAC 81
           L   N  PLS D ++ LP E F++DA  LAPRLLGK LRRDG+VL+ITEVEAYR ND+AC
Sbjct: 58  LTIPNSNPLSFDKMRILPLEFFQIDALDLAPRLLGKFLRRDGVVLQITEVEAYRPNDSAC 117

Query: 82  HGRFGVTARTTP 93
           HGRFG+TART P
Sbjct: 118 HGRFGITARTAP 129

[Close]

BLAST of CmaCh06G001110 vs. ExPASy TrEMBL
Match: A0A446VPM3 (3-methyladenine DNA glycosidase OS=Triticum turgidum subsp. durum OX=4567 GN=TRITD_6Av1G195730 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.9e-17
Identity = 47/64 (73.44%), Postives = 52/64 (81.25%), Query Frame = 0


Query: 29  PLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACHGRFGVTA 88
           P S+  + LP E F VDA  LAPRLLGKLLRRD +VLRITEVEAYR ND+ACHGRFG+TA
Sbjct: 95  PQSAAGMALPREFFEVDALDLAPRLLGKLLRRDQVVLRITEVEAYRPNDSACHGRFGITA 154

Query: 89  RTTP 93
           RT P
Sbjct: 155 RTAP 158

[Close]

BLAST of CmaCh06G001110 vs. ExPASy TrEMBL
Match: A0A3B6NUK3 (3-methyladenine DNA glycosidase OS=Triticum aestivum OX=4565 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.9e-17
Identity = 47/64 (73.44%), Postives = 52/64 (81.25%), Query Frame = 0


Query: 29  PLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACHGRFGVTA 88
           P S+  + LP E F VDA  LAPRLLGKLLRRD +VLRITEVEAYR ND+ACHGRFG+TA
Sbjct: 95  PQSAAGMALPREFFEVDALDLAPRLLGKLLRRDQVVLRITEVEAYRPNDSACHGRFGITA 154

Query: 89  RTTP 93
           RT P
Sbjct: 155 RTAP 158

[Close]

BLAST of CmaCh06G001110 vs. NCBI nr
Match: XP_022983051.1 (DNA-3-methyladenine glycosylase [Cucurbita maxima])

HSP 1 Score: 100.5 bits (249), Expect = 8.0e-18
Identity = 50/79 (63.29%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 14  PISAHKSKLLYSNPLPLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAY 73
           P++  ++++  S+P   S     LP+E F VDA  LAPRLLGKLLRRD +VLRITEVEAY
Sbjct: 78  PVTDPQTQIAQSSPSSSSEGFTILPKEFFLVDALDLAPRLLGKLLRRDDVVLRITEVEAY 137

Query: 74  RQNDTACHGRFGVTARTTP 93
           R ND+ACHGRFGVTART P
Sbjct: 138 RPNDSACHGRFGVTARTAP 156

[Close]

BLAST of CmaCh06G001110 vs. NCBI nr
Match: KAG8072604.1 (hypothetical protein GUJ93_ZPchr0006g42996 [Zizania palustris])

HSP 1 Score: 100.5 bits (249), Expect = 8.0e-18
Identity = 49/78 (62.82%), Postives = 59/78 (75.64%), Query Frame = 0


Query: 15  ISAHKSKLLYSNPLPLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYR 74
           ++A +++    +P PLS     LP + F VDA  LAPRLLGKLLRRD +VLRITEVEAYR
Sbjct: 33  VAAGEAEAAGRSPRPLSPIGRALPRDFFEVDALDLAPRLLGKLLRRDEVVLRITEVEAYR 92

Query: 75  QNDTACHGRFGVTARTTP 93
            ND+ACHGRFG+TART P
Sbjct: 93  PNDSACHGRFGITARTAP 110

[Close]

BLAST of CmaCh06G001110 vs. NCBI nr
Match: TVU28754.1 (hypothetical protein EJB05_20286, partial [Eragrostis curvula])

HSP 1 Score: 100.5 bits (249), Expect = 8.0e-18
Identity = 51/71 (71.83%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 27  PLPLSSDSL-----KLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACH 86
           P PL SD+L      LP+E F VDA  LAPRLLGKLLRRD +VLRITEVEAYR ND+ACH
Sbjct: 99  PAPLVSDALPHIGRALPQEFFEVDALDLAPRLLGKLLRRDEVVLRITEVEAYRPNDSACH 158

Query: 87  GRFGVTARTTP 93
           GRFG+TART P
Sbjct: 159 GRFGITARTAP 169

[Close]

BLAST of CmaCh06G001110 vs. NCBI nr
Match: XP_021669210.1 (DNA-3-methyladenine glycosylase [Hevea brasiliensis] >KAF2289513.1 hypothetical protein GH714_036770 [Hevea brasiliensis])

HSP 1 Score: 99.0 bits (245), Expect = 2.3e-17
Identity = 49/72 (68.06%), Postives = 57/72 (79.17%), Query Frame = 0


Query: 22  LLYSNPLPLSSDSLK-LPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTAC 81
           L   N  PLS D ++ LP E F++DA  LAPRLLGK LRRDG+VL+ITEVEAYR ND+AC
Sbjct: 58  LTIPNSNPLSFDKMRILPLEFFQIDALDLAPRLLGKFLRRDGVVLQITEVEAYRPNDSAC 117

Query: 82  HGRFGVTARTTP 93
           HGRFG+TART P
Sbjct: 118 HGRFGITARTAP 129

[Close]

BLAST of CmaCh06G001110 vs. NCBI nr
Match: XP_023527732.1 (DNA-3-methyladenine glycosylase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 98.6 bits (244), Expect = 3.0e-17
Identity = 50/79 (63.29%), Postives = 59/79 (74.68%), Query Frame = 0


Query: 14  PISAHKSKLLYSNPLPLSSDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAY 73
           P +  ++++  S+P   S     LP+E F VDA  LAPRLLGKLLRRD +VLRITEVEAY
Sbjct: 78  PATDPQTQIAESSPSSSSEGFTILPKEFFLVDALDLAPRLLGKLLRRDDVVLRITEVEAY 137

Query: 74  RQNDTACHGRFGVTARTTP 93
           R ND+ACHGRFGVTART P
Sbjct: 138 RPNDSACHGRFGVTARTAP 156

[Close]

BLAST of CmaCh06G001110 vs. TAIR 10
Match: AT3G12040.1 (DNA-3-methyladenine glycosylase (MAG) )

HSP 1 Score: 91.3 bits (225), Expect = 4.5e-19
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 32  SDSLKLPEELFRVDAWGLAPRLLGKLLRRDGLVLRITEVEAYRQNDTACHGRFGVTARTT 91
           S+   +P E F++DA  LAPRLLGK +RRD +VLRITEVEAYR ND+ACHGRFGVT RT 
Sbjct: 54  SEMKLMPPEFFQIDALDLAPRLLGKFMRRDNVVLRITEVEAYRPNDSACHGRFGVTPRTA 113

Query: 92  P 93
           P
Sbjct: 114 P 114

The following BLAST results are available for this feature:

BLAST of CmaCh06G001110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39147	6.4e-18	68.85	DNA-3-methyladenine glycosylase OS=Arabidopsis thaliana OX=3702 GN=MAG PE=2 SV=1	[more]
Q3JEY0	1.9e-06	54.39	Putative 3-methyladenine DNA glycosylase OS=Nitrosococcus oceani (strain ATCC 19...	[more]

BLAST of CmaCh06G001110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J112	3.9e-18	63.29	3-methyladenine DNA glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111481712 PE=3...	[more]
A0A5J9UZT0	3.9e-18	71.83	3-methyladenine DNA glycosidase (Fragment) OS=Eragrostis curvula OX=38414 GN=EJB...	[more]
A0A6A6KNC4	1.1e-17	68.06	3-methyladenine DNA glycosidase OS=Hevea brasiliensis OX=3981 GN=GH714_036770 PE...	[more]
A0A446VPM3	1.9e-17	73.44	3-methyladenine DNA glycosidase OS=Triticum turgidum subsp. durum OX=4567 GN=TRI...	[more]
A0A3B6NUK3	1.9e-17	73.44	3-methyladenine DNA glycosidase OS=Triticum aestivum OX=4565 PE=3 SV=1	[more]

BLAST of CmaCh06G001110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983051.1	8.0e-18	63.29	DNA-3-methyladenine glycosylase [Cucurbita maxima]	[more]
KAG8072604.1	8.0e-18	62.82	hypothetical protein GUJ93_ZPchr0006g42996 [Zizania palustris]	[more]
TVU28754.1	8.0e-18	71.83	hypothetical protein EJB05_20286, partial [Eragrostis curvula]	[more]
XP_021669210.1	2.3e-17	68.06	DNA-3-methyladenine glycosylase [Hevea brasiliensis] >KAF2289513.1 hypothetical ...	[more]
XP_023527732.1	3.0e-17	63.29	DNA-3-methyladenine glycosylase [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh06G001110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12040.1	4.5e-19	68.85	DNA-3-methyladenine glycosylase (MAG)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036995	Methylpurine-DNA glycosylase superfamily	GENE3D	3.10.300.10		coord: 32..93 e-value: 4.9E-13 score: 50.7
IPR003180	Methylpurine-DNA glycosylase	PFAM	PF02245	Pur_DNA_glyco	coord: 41..91 e-value: 2.4E-10 score: 40.1
IPR003180	Methylpurine-DNA glycosylase	PANTHER	PTHR10429	DNA-3-METHYLADENINE GLYCOSYLASE	coord: 31..91
IPR011034	Formyl transferase-like, C-terminal domain superfamily	SUPERFAMILY	50486	FMT C-terminal domain-like	coord: 36..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G001110.1	CmaCh06G001110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006284	base-excision repair
molecular_function	GO:0008725	DNA-3-methyladenine glycosylase activity
molecular_function	GO:0052822	DNA-3-methylguanine glycosylase activity
molecular_function	GO:0052821	DNA-7-methyladenine glycosylase activity
molecular_function	GO:0043916	DNA-7-methylguanine glycosylase activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003905	alkylbase DNA N-glycosylase activity
molecular_function	GO:0003824	catalytic activity