CmaCh06G000590 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G000590
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	membrane steroid-binding protein 2-like
Location	Cma_Chr06: 332585 .. 334743 (+)
RNA-Seq Expression	CmaCh06G000590
Synteny	CmaCh06G000590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGGAGTAGGTAAGGATTCATGGTTGGTGAAGTTGGGACTTTTATCTTCAAATCCAGTCCACCGGAATCTATTGCCTCGGGGAGAAAATCGCAGTCGCAAAGCTAGAGCGAAGAAATGGAGATCTTCTGGAGAGCGATGGAACAGATCAATTGGTACACCGGATTATCGCCCACCGCCTTCTTCACCATACTTGCCTCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAACCCCCAACTGTTCCTGTTTCTTCGTCTAACTCGGCCAATTCGAATTTGTTTGTTAACGATACGGTCGCCGACGCCTCGCAATCAGTCCAAGTAGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACGATGGCTCCGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTATCTTCCGGCAGGTACTTTTTTTTTCCTTCATCATTCGTGGAGCGAATTCCGTTTGGTTTCTATTTAGAAATTTGCTCATAACTTGCATAGTTGCCTACTTTCATGAGTTGAAGCATTGAGTATGAAAATTCTTCTTATTCTTCGCTCTAGATGAAGAACTACTCCTGTTTATCTCATGACTGGAGTTCAAGAATTGCAGAATTTTAAACAAAACTGACCGACCGGCGATATTTATTGAGGATATACATTAGAATTTCTTGAGCACACTTCCAGTTGTAATCCCATTTACACTACTTGTCAATTGTCATATTGTCTGTTTTACGTCAAAGTTCCACTGATCAGCTCAAACTGTTAAGATCTTTGGCCAAGATTTTCAAGGAGGAGAATATTTTTGCACAAAATGTGTTGGCGAGAGGATTGTCAGGCCTTCCATTCGGTTTCCTTGTGTGAGAAAAAGGGTGGTCGGGAGTAAAGAATGAAAGTTGGGCTGTGTGGGACTGGGGACTGAAGTTGCAGAAGGGAAGGGGAAGTTTTTGTTTCCTCTCTGTGAAAGCGGCTAATAACAGTAATTTTGTCAGTCAACCAGTCTGTTGGGGAATATTAAACTCATTGTCGAGTTTAGGAAGCTTTAGGGTTGGAAAGCCTCTCCATATTTACATAATACATGGCTGGATTTGTGAGATCTCACATCGGTTGGAGAGGGAAACGAAACATTCCTTATAAGTGTGTGGAAACCTCTTCCTAGTGGACGCGTTTAAAAACCTAGAGGGAAAGCCTAGAAGGGAAAGCTCAAAGAGGACAATATCTACTAGCGTGGGCTTGGGTTGTTACAAATGGTATCAGAGGCAGACATCGGGTGGTGTGTCATTGAGGACGTTGGGCCACAAAGGGGTGGATTGTGAGATCCCACGTCGGTTGGAGAGGGGAACGAAGCATTCTTATAAGGTGTGGAAACATCTCCCTAGTGGACACGTTTTAAAATCTTAAGGGGAAGCCCATAAGGGAAAGCGGACAATATCTGCTAGCGGTGAGCTTGGGTTGTTACAGGATTGCTCAAATCAATGAACTATAAAGGATTTAGTATACAAAAAGAGTTTCCTAAGCTTACGTTTATGTAACCTAATTCTGGACAAAATTGAAACTTCTTAAGAGTTCAAAGACTAAATTGAAAGATGTAATTGCATAGGATGACACTGTGTTTTTGGTTGGCTTTTCAGGACGTTTTATGGTCCTGGTTCACCTTACTCCATGTTCGTCGGGAAGGATGCTAGCAGAGCCTTGGCTCTCTTGTCTTTTAAACCTGAAGACATTAATGGTAATCTCGAAGGCCTGAATGAAGAAGAGCTTGTAATCTTGCAGGATTGGGAATACAAGTTCATGGAAAAGTATGTGAAGGTTGGGGAGCTCGTTTCAGAGGAGGCATTGAATGAGCGTTCTGAAAATGGACACCAAAGTACCGACACCAATCGAGAAGAAGAACCCAACAAAGATCAATAAGAAGGCGTTTATTGCTTTCACCTTTCATCAGGCTCATCACTGAAAGTTCTTGACTTGAATGTGCCTGTCCTTTGCCCTTCAATCGGATTGATCACATTGAAGTTAGATATCCTGCTACATCTTGGCTACATTTTTGGTTTTGTATATTACTTCACACGAAAATTTTTAATACGAGCAAAAGTTTACATTACAAAATTTTCAATTTTGACATTTATCG

mRNA sequence

AGGGAGTAGGTAAGGATTCATGGTTGGTGAAGTTGGGACTTTTATCTTCAAATCCAGTCCACCGGAATCTATTGCCTCGGGGAGAAAATCGCAGTCGCAAAGCTAGAGCGAAGAAATGGAGATCTTCTGGAGAGCGATGGAACAGATCAATTGGTACACCGGATTATCGCCCACCGCCTTCTTCACCATACTTGCCTCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAACCCCCAACTGTTCCTGTTTCTTCGTCTAACTCGGCCAATTCGAATTTGTTTGTTAACGATACGGTCGCCGACGCCTCGCAATCAGTCCAAGTAGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACGATGGCTCCGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTATCTTCCGGCAGGACGTTTTATGGTCCTGGTTCACCTTACTCCATGTTCGTCGGGAAGGATGCTAGCAGAGCCTTGGCTCTCTTGTCTTTTAAACCTGAAGACATTAATGGTAATCTCGAAGGCCTGAATGAAGAAGAGCTTGTAATCTTGCAGGATTGGGAATACAAGTTCATGGAAAAGTATGTGAAGGTTGGGGAGCTCGTTTCAGAGGAGGCATTGAATGAGCGTTCTGAAAATGGACACCAAAGTACCGACACCAATCGAGAAGAAGAACCCAACAAAGATCAATAAGAAGGCGTTTATTGCTTTCACCTTTCATCAGGCTCATCACTGAAAGTTCTTGACTTGAATGTGCCTGTCCTTTGCCCTTCAATCGGATTGATCACATTGAAGTTAGATATCCTGCTACATCTTGGCTACATTTTTGGTTTTGTATATTACTTCACACGAAAATTTTTAATACGAGCAAAAGTTTACATTACAAAATTTTCAATTTTGACATTTATCG

Coding sequence (CDS)

ATGGAGATCTTCTGGAGAGCGATGGAACAGATCAATTGGTACACCGGATTATCGCCCACCGCCTTCTTCACCATACTTGCCTCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAACCCCCAACTGTTCCTGTTTCTTCGTCTAACTCGGCCAATTCGAATTTGTTTGTTAACGATACGGTCGCCGACGCCTCGCAATCAGTCCAAGTAGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACGATGGCTCCGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTATCTTCCGGCAGGACGTTTTATGGTCCTGGTTCACCTTACTCCATGTTCGTCGGGAAGGATGCTAGCAGAGCCTTGGCTCTCTTGTCTTTTAAACCTGAAGACATTAATGGTAATCTCGAAGGCCTGAATGAAGAAGAGCTTGTAATCTTGCAGGATTGGGAATACAAGTTCATGGAAAAGTATGTGAAGGTTGGGGAGCTCGTTTCAGAGGAGGCATTGAATGAGCGTTCTGAAAATGGACACCAAAGTACCGACACCAATCGAGAAGAAGAACCCAACAAAGATCAATAA

Protein sequence

MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSANSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSEEALNERSENGHQSTDTNREEEPNKDQ

Homology

[Close]

BLAST of CmaCh06G000590 vs. ExPASy Swiss-Prot
Match: Q9M2Z4 (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.8e-42
Identity = 95/198 (47.98%), Postives = 125/198 (63.13%), Query Frame = 0


Query: 2   EIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSAN 61
           +I+    E I  YTGLSP AFFT+LA    V+Q+VS  FVSP E ++P ++ V   +   
Sbjct: 4   QIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSP-EVHRPRSLEVQPQSE-- 63

Query: 62  SNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGS 121
                          VQ+G +TE++L+ YDGSD  KPLLMAIKGQIYDVS  R FYGPG 
Sbjct: 64  ----------PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGG 123

Query: 122 PYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSEE 181
           PY++F GKDASRALA +SF+ +D+ G++ GL   EL  LQDWEYKFM KYVKVG +  ++
Sbjct: 124 PYALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKD 183

Query: 182 ALNERSENGHQSTDTNRE 200
              + S    ++   + E
Sbjct: 184 GEGKESSEPSEAKTASAE 188

[Close]

BLAST of CmaCh06G000590 vs. ExPASy Swiss-Prot
Match: Q9XFM6 (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-39
Identity = 88/175 (50.29%), Postives = 112/175 (64.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           +E++    E I+ YTGLSP  FFT LA    ++Q++S  F SP +        V+    A
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD-------DVNRHQRA 62

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
            S     +      Q VQVG +TE++L+ YDGSDP KPLLMAIK QIYDV+  R FYGPG
Sbjct: 63  RS--LAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVG 176
            PY++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168

[Close]

BLAST of CmaCh06G000590 vs. ExPASy Swiss-Prot
Match: Q9FVZ7 (Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP1 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-39
Identity = 94/202 (46.53%), Postives = 125/202 (61.88%), Query Frame = 0


Query: 5   WRAMEQ-INWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSANSN 64
           W  ++Q I  YTGLSP AFFT +A+   ++ +VS +F  P     PP  P    +   + 
Sbjct: 9   WETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGP-----PPPPPPRPRDEPEAE 68

Query: 65  LFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPY 124
                        VQ+G ++E++LR YDGSDP KPLLMAIKGQIYDV+  R FYGPG PY
Sbjct: 69  --------PLPPPVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPY 128

Query: 125 SMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSEEAL 184
           ++F GKDASRALA +SF+P+D+ G++ GL   EL  LQDWEYKFM KYVKVG +     +
Sbjct: 129 ALFAGKDASRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVGTVKKTVPV 188

Query: 185 NERS-ENGHQSTDTNREEEPNK 205
            + +     ++T+T    EP K
Sbjct: 189 EDGAPSTSPETTETAAAAEPEK 197

[Close]

BLAST of CmaCh06G000590 vs. ExPASy Swiss-Prot
Match: Q9FVZ9 (Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP2 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.8e-34
Identity = 77/160 (48.12%), Postives = 99/160 (61.88%), Query Frame = 0


Query: 18  SPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSANSNLFVNDTVADASQSV 77
           SP A    +A    V+++ SS+   P    +P   P             ++      + V
Sbjct: 70  SPVAVIAAVAGAAVVYKVGSSLLAPPPPPARPREEP-------------SEEAPPPPEPV 129

Query: 78  QVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALAL 137
           QVG +T ++L  YDGSDP KPLLMAIKGQIYDVS  R FYGPG PY++F GKDASRALA 
Sbjct: 130 QVGEITAEELLQYDGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRALAK 189

Query: 138 LSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGEL 178
           +SF+P+D+  ++ GL+  EL  LQDWEYKF  KYVKVG +
Sbjct: 190 MSFEPQDLTDDISGLSLLELSALQDWEYKFSSKYVKVGTI 216

[Close]

BLAST of CmaCh06G000590 vs. ExPASy Swiss-Prot
Match: Q9SK39 (Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.5e-24
Identity = 52/97 (53.61%), Postives = 72/97 (74.23%), Query Frame = 0


Query: 83  TEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKP 142
           T +QL  Y+G+D +KP+ +AIKG+++DV++G++FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 143 EDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVS 180
           ED++ +LEGL E+E+  L DWE KF  KY  VG +VS
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVVS 100

[Close]

BLAST of CmaCh06G000590 vs. ExPASy TrEMBL
Match: A0A6J1I932 (membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470292 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.3e-108
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNERSENGHQSTDTNREEEPNKDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. ExPASy TrEMBL
Match: A0A6J1GGB3 (membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453933 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.7e-106
Identity = 203/206 (98.54%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFV+D+VADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLE LNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNERSENGHQSTDTNREEEPNKDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. ExPASy TrEMBL
Match: A0A6J1DCX2 (membrane steroid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111019874 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.8e-92
Identity = 173/200 (86.50%), Postives = 186/200 (93.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           ME +W+AMEQI+WYTGLSP AFFTILASMIFVFQMVSSMFVSPEEF+KPPTVP+SSSN A
Sbjct: 1   MEFYWKAMEQISWYTGLSPMAFFTILASMIFVFQMVSSMFVSPEEFSKPPTVPISSSNPA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFVND VADASQ+VQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGR FYGPG
Sbjct: 61  NSNLFVNDAVADASQAVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPY+MF GKDASRALALLSF+PEDINGN+EGL+EEELVILQDWEYKFMEKY+KVGELV E
Sbjct: 121 SPYAMFTGKDASRALALLSFRPEDINGNIEGLSEEELVILQDWEYKFMEKYIKVGELVPE 180

Query: 181 EALNERSENGHQSTDTNREE 201
           E LNE SEN H   +T +EE
Sbjct: 181 EGLNEHSENEHPKVETGQEE 200

[Close]

BLAST of CmaCh06G000590 vs. ExPASy TrEMBL
Match: A0A5A7UDA3 (Membrane steroid-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006170 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.6e-91
Identity = 179/207 (86.47%), Postives = 193/207 (93.24%), Query Frame = 0


Query: 1   MEIFWR-AMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNS 60
           ME +WR AMEQI WYTGLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSNS
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  ANSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGP 120
           +NSNLFVND+ ADASQ+V VGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVS 180
           GSPYSMFVGKDASRALALLSFKPEDINGN+EGL+EEELVILQDWEYKFMEKYVKVGELV 
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EEALNERSENGHQSTDTNREEEPNKDQ 207
           E  LNE SENGHQ+++T + E  +KDQ
Sbjct: 181 EGELNEHSENGHQNSETTQVEH-DKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. ExPASy TrEMBL
Match: E5GBX8 (Steroid binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.6e-91
Identity = 179/207 (86.47%), Postives = 193/207 (93.24%), Query Frame = 0


Query: 1   MEIFWR-AMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNS 60
           ME +WR AMEQI WYTGLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSNS
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  ANSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGP 120
           +NSNLFVND+ ADASQ+V VGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVS 180
           GSPYSMFVGKDASRALALLSFKPEDINGN+EGL+EEELVILQDWEYKFMEKYVKVGELV 
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EEALNERSENGHQSTDTNREEEPNKDQ 207
           E  LNE SENGHQ+++T + E  +KDQ
Sbjct: 181 EGELNEHSENGHQNSETTQVEH-DKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. NCBI nr
Match: XP_022971619.1 (membrane steroid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 401.4 bits (1030), Expect = 4.8e-108
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNERSENGHQSTDTNREEEPNKDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. NCBI nr
Match: KAG7027737.1 (Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 397.9 bits (1021), Expect = 5.3e-107
Identity = 204/206 (99.03%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFV+D+VADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNERSENGHQSTDTNREEEPNKDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. NCBI nr
Match: KAG6596198.1 (Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 396.4 bits (1017), Expect = 1.6e-106
Identity = 203/206 (98.54%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFV+D+VADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNERSENGH+STDTNREEEPNKDQ
Sbjct: 181 EALNERSENGHRSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. NCBI nr
Match: XP_023538658.1 (membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395.2 bits (1014), Expect = 3.5e-106
Identity = 203/206 (98.54%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFV+D+VADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNE SENGHQSTDTNREEEPNKDQ
Sbjct: 181 EALNEPSENGHQSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. NCBI nr
Match: XP_022950957.1 (membrane steroid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 395.2 bits (1014), Expect = 3.5e-106
Identity = 203/206 (98.54%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NSNLFV+D+VADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG
Sbjct: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180
           SPYSMFVGKDASRALALLSFKPEDINGNLE LNEEELVILQDWEYKFMEKYVKVGELVSE
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 EALNERSENGHQSTDTNREEEPNKDQ 207
           EALNERSENGHQSTDTNREEEPNKDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

[Close]

BLAST of CmaCh06G000590 vs. TAIR 10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3 )

HSP 1 Score: 172.6 bits (436), Expect = 3.4e-43
Identity = 95/198 (47.98%), Postives = 125/198 (63.13%), Query Frame = 0


Query: 2   EIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSAN 61
           +I+    E I  YTGLSP AFFT+LA    V+Q+VS  FVSP E ++P ++ V   +   
Sbjct: 4   QIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSP-EVHRPRSLEVQPQSE-- 63

Query: 62  SNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGS 121
                          VQ+G +TE++L+ YDGSD  KPLLMAIKGQIYDVS  R FYGPG 
Sbjct: 64  ----------PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGG 123

Query: 122 PYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSEE 181
           PY++F GKDASRALA +SF+ +D+ G++ GL   EL  LQDWEYKFM KYVKVG +  ++
Sbjct: 124 PYALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKD 183

Query: 182 ALNERSENGHQSTDTNRE 200
              + S    ++   + E
Sbjct: 184 GEGKESSEPSEAKTASAE 188

[Close]

BLAST of CmaCh06G000590 vs. TAIR 10
Match: AT5G52240.1 (membrane steroid binding protein 1 )

HSP 1 Score: 164.5 bits (415), Expect = 9.2e-41
Identity = 88/175 (50.29%), Postives = 112/175 (64.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           +E++    E I+ YTGLSP  FFT LA    ++Q++S  F SP +        V+    A
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD-------DVNRHQRA 62

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
            S     +      Q VQVG +TE++L+ YDGSDP KPLLMAIK QIYDV+  R FYGPG
Sbjct: 63  RS--LAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVG 176
            PY++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168

[Close]

BLAST of CmaCh06G000590 vs. TAIR 10
Match: AT5G52240.2 (membrane steroid binding protein 1 )

HSP 1 Score: 164.5 bits (415), Expect = 9.2e-41
Identity = 88/175 (50.29%), Postives = 112/175 (64.00%), Query Frame = 0


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           +E++    E I+ YTGLSP  FFT LA    ++Q++S  F SP +        V+    A
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD-------DVNRHQRA 62

Query: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
            S     +      Q VQVG +TE++L+ YDGSDP KPLLMAIK QIYDV+  R FYGPG
Sbjct: 63  RS--LAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVG 176
            PY++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168

[Close]

BLAST of CmaCh06G000590 vs. TAIR 10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2 )

HSP 1 Score: 112.8 bits (281), Expect = 3.2e-25
Identity = 52/97 (53.61%), Postives = 72/97 (74.23%), Query Frame = 0


Query: 83  TEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKP 142
           T +QL  Y+G+D +KP+ +AIKG+++DV++G++FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 143 EDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVS 180
           ED++ +LEGL E+E+  L DWE KF  KY  VG +VS
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVVS 100

[Close]

BLAST of CmaCh06G000590 vs. TAIR 10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4 )

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-14
Identity = 36/116 (31.03%), Postives = 62/116 (53.45%), Query Frame = 0


Query: 63  NLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSP 122
           +L+   +        Q    + ++L  Y+G+D   P+L+ I G ++DV+ G+  YG G  
Sbjct: 23  SLYFRSSFKSPQHQYQKRLFSAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGG 82

Query: 123 YSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELV 179
           Y+ F G+DASRA    +F  + +  +L+GL+  E+  + DW   +   Y  VG+LV
Sbjct: 83  YNHFAGRDASRAFVSGNFTGDGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLV 138

The following BLAST results are available for this feature:

BLAST of CmaCh06G000590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2Z4	4.8e-42	47.98	Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1...	[more]
Q9XFM6	1.3e-39	50.29	Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1...	[more]
Q9FVZ7	1.3e-39	46.53	Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9FVZ9	2.8e-34	48.13	Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9SK39	4.5e-24	53.61	Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 S...	[more]

BLAST of CmaCh06G000590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I932	2.3e-108	100.00	membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A6J1GGB3	1.7e-106	98.54	membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1DCX2	6.8e-92	86.50	membrane steroid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5A7UDA3	2.6e-91	86.47	Membrane steroid-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
E5GBX8	2.6e-91	86.47	Steroid binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of CmaCh06G000590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022971619.1	4.8e-108	100.00	membrane steroid-binding protein 2-like [Cucurbita maxima]	[more]
KAG7027737.1	5.3e-107	99.03	Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6596198.1	1.6e-106	98.54	Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023538658.1	3.5e-106	98.54	membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022950957.1	3.5e-106	98.54	membrane steroid-binding protein 2-like [Cucurbita moschata]	[more]

BLAST of CmaCh06G000590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48890.1	3.4e-43	47.98	membrane-associated progesterone binding protein 3	[more]
AT5G52240.1	9.2e-41	50.29	membrane steroid binding protein 1	[more]
AT5G52240.2	9.2e-41	50.29	membrane steroid binding protein 1	[more]
AT2G24940.1	3.2e-25	53.61	membrane-associated progesterone binding protein 2	[more]
AT4G14965.1	1.9e-14	31.03	membrane-associated progesterone binding protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 82..178 e-value: 1.5E-18 score: 77.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 83..177 e-value: 1.4E-13 score: 50.8
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 80..179 e-value: 6.9E-36 score: 124.5
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 80..178
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 182..206
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 6..201
None	No IPR available	PANTHER	PTHR10281:SF45	MEMBRANE STEROID-BINDING PROTEIN 2	coord: 6..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G000590.1	CmaCh06G000590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane