CmaCh06G000550 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G000550
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	NADH:cytochrome B5 reductase 1
Location	Cma_Chr06: 301096 .. 305913 (+)
RNA-Seq Expression	CmaCh06G000550
Synteny	CmaCh06G000550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATTCCTTTGACATTTTCTGGATCTCCATCATCATTTACTTGACCAGGACTCAAATCCAACTCCTTAAACCCACCATTTTTCTTCCTTCTTACCTACCCCTCATTCTTCCAAGTCTCCCAATTTCTGATGGAGATCTTACAGGCTCTCGATGCTCAGGTTCTCACTGGCTTAGCCGCCGCCCTGGTTGCCATTGTTGCTGGTGCCTTTTTCCTTGTCTCCTCCAGAAAACCAGCATCCAGAGGTTTTACTCCACCTTCTGATTTGATTCTTTGAAATGAAAGCCTTATGGGTCTTTATTTACTGGTTTGGTTTATTGGGTGTTTTAAATTCTCTATCCAGTTCGAATTTGTGATGCTCTGATGTTTTGATTCGTGGGTTGTGATGTTTTTGAACTGTGTTGAAGTATTTATTAAGATTGAATTTATACTGATCCATTAGCTTAACCTTTTACTTCTTATGATGATTTTACATTGGTATAAGGGAGGAATTATCTCCTCCTTGATTAATATTGATAGCTACATGTTTTGATTACCACCTCCGGTAGATATTGTCTACTTTAGCCCGTTAAGTATCACCGTCAGCCTCACGGTTTTTAAAACGTGTCTATTAGGGAGAGGTTTCCACACCCTTATAAGAAATGTTTCGTTCCCTTCTCAAACCGAGGCAGGATCTCACAATCCTTGGGGGCCAAATGCCCTTTCTGCACACCGCTCAGTGTCTTTACCATTTGTAACAACCCAAGCTCACCACTAGTAGATATTGTCCGCTTTTGCCCATTACGTATTGCCATTAGTCTCATGGTTTTTAAAATGTGTTTGCTAAGAAGAGGTTTCCACACCCTTGTAAGGAATGCTTTGTTTCCCTCTTCAACCGATGCGGGATCTCACAATCCTTTGGGGCCAAATGTCCTTGCTGGCACACCACCCGGTGTCTAGCTCTACCATTTGTAACAGCCCAAGCTCACCGCTAGTAGATATCGTCCACTTTGGCCCATTACATATTGCCATCAGCCGCACGGTTTTTAAACGCGCTTGCTAGGGAGAGGTTTCCACACCCTTATAAGGAATGCTTTGTTCCCCTCTTCAACCGATGCAGTATCTCACAATCCTTGGGGGCCCAACATCCTCGCTGGCACACCGCCCGGTGTCTAGCTCTATCATTTGTAACAGCCCAAGCCCACCGTTAGTAGATATCATCCACTTTAGCCCGTTATGTATTGCCGTTAACCTCACGGTTTTTAAAACGCATATGCTAGGGAGAGGTTTCCACACCCCTATAAAGAATGATTCATTCCCCTCTCCAACCACTCACAAATTTAGCATAGAAGTGGTAGCATTTGCAGAAAAAGGTCAAGTGCTTAGCTAGGCCTTTGGACGTGTTTAAATTTCAAACTAGCTAATTGATCAAAATTCTAATGGCCATGAAAATTATCTTCATCTTTGTTGAGCTCTTGGTCAATGTGAAATGGTAGATGTTATGGGAAATATGCATTTTAAAAAAGATAATGCTCTTTTCTTGCCCTGACTCTTATGAGAGGATTAACACCTATAATGTTATCTAATTAGCAGCTAATGTTCTTGAACCATCTTTTCTTGATATCATCACAATTTCTTGATATCTTAATAAAATTGATTGGTTCATATGTGGCATGTGGTTTGTGACTACAGCCTGCTTGGATCCTGATAACTTTAAGGAGTTTAAACTTGTTAAGCGCACGAAGCTGAGCCATAATGTGGCAAAGTTCATTTTTATACTTCCCAAGACCGATTCAATTCTTGGTCTTCCTATTGGACAGCATCTAAGTTGCAGGTTTCATTTTATCCAATATATTTGTTTTCCCAGCCATTATAGCGATGCAGCTATGATACTCAGCAGTTAACACACCTAATATTTGGTTCATTTTCCCTCTGTTTAAAATCATATGTGCAGGGGAAAGGATAGCCAAGGTGAAGAGGTTATTAAATCTTACACCCCAGTTACCTTGGACTCTGATGTTGGATATTTTGAGCTAGTCATAAAGGTACATATATAAATCTGCTACAGGTTAATGATGCCACATTGATTATCATTATTCCACTTCTGATTCTTATATGATTGTGTGTGTGATTTAGATGTATCCCCAAGGAAGGATGTCGCATCATTTTCGAGAAATGCGTATGGGGGACTTTCTTGCTGTGAAGGGACCAAAGGTATGTCTTCCTTACATACAAACTTTGACAACAGTTTTGGAATGTGTTATTGTTTGATTCTGTAAGAAGCTTTTGTTTGAGTGACTGAATTTCCCTTGATTTGGATTGAACTCGATAGTGATTGTGATCTGTTAATCTAAATGATGTTTTGTCCTCTCGGTGTTGATTACTTTTTGTATTATGTTTCTTCTGTTGAGATATTGTGTTGTTCATGGTGAAGGAGATATACAACATTATTGTACATATTGTCCTCTTTGGGCTTTCCCTTTCGAGCTTCCCCTCAAGGTTTTTGAAACGCGTCTGTTATGAAAAGGTTTTCACACCCTCATAAAGAATGTTTCATTCCCCTTTTGGGCTTTCCCTTTCGAGCTTCCCCTCAAGGTTTTTGAAACGCGTCTGTTATGAAGAGGTTTTCACACCCTCATAAAGAATGCTTCATTCCCCTCCCCAACCGATGTGGGATCTCACAATTAACGTTCTTGCTGACACACCGCCTTGTGTCCACCCCCCTTCGGGGCTTAGCCTCCTCGCTGGCACATTGCCCGGTGTCTGGCTTTGATACCATTTGTAACGGCCCAAGTCCACCGCTAGCAGATATTGTCCACTTTGGGCTTTCTCTTTCGGGCTTCCCCTCAAGGTTTTTAAATCGTGTCGGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAACCGATATGGGATCTCACAATCCACTCTCCTTCAGAGCCAGCATCCTTACTGGCACTCGTTTCTTTCTCCAATCAATGTGGGACCCCCAAATCCACCCCCTTCCGAGCTCAGCCTTCTCCATGGCATGTCACCCAGTGTCTAGCTCAGATACCATTTGTAACGGTCCAAGCCCATCACTATGTCCTCTTTAGACTTTCTCTTTCGGACTTCCCCTCAAGGTTTTTATATCGCGTCGACTAGGGAGAAGTTTCCACACCCTCATACAGAATGCTTCGTTCTTCTCCCCAACCAATGTGAGATTAGATACACAAACACATAAAAACACACGATAGTAAATGTCAAAGACAAATTGCAAAATGACTAACCTTTCGAGAGGAGCATCTCTCTCCCAAGTCCCACTAAAATGATCTACCACAAAACTAAGCTCCCTAAAACTTCTATTTCTTTAACCAAGACACCTCTAACTAATAACAACTATGCCCTTATTAATAACATACCAATCCCCCACGTAGGATCTATGTCCTTATAGAATTGAGTTGCTTTGAATGCTGCCTATTTTAGAAATTTATGTACTCCATCAATTATTGCAGGGAAGTTTCAAGTATCAACCTGGTAAAGTTAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACACCCATGTATCAAGTAATGAAGCAAACTTTTCTTCAACAACACATTTTCCATTATTCTTGGAATCTTGGCCCTATTTTGTTGTTTCATTGTCTCTTGTCATGTTTTAAAACAAGACGAAACTCTCTCATTTTTACTTCTGTTCTAGGTTGCTCGGTCTATATTAGAAAACCCGAACGACAAGACAAAGATTCACCTTATTTATGCCAATGTTACACTTGAAGATATTCTTTTGAAGGTAATTATTTTCTTTGTTTTTTTCTTCAACTTCTTACTTTCTTCCCATTAGAAATACATAACAATACAATAATCACTTGTATTTGCAGGAGGAATTGGATCTTCTTGCCAAGAGATACCCTGATCGCTTCAAGATCTACTATGTGTTAAACCAGGTCAGTTAAAAGTTTATAAACTTAGTATTAATCTAATACAATGGTACGAGGATACTGAGAATATATTAAATAACGACTTTAGAGTATTTGTATAATGTTTAATGTTACTACTCATGTTCTTTGGACATTGAAATAGCCTCCTAAAGTTTGGGATGGCGGCGTCGGCTTTGTGTCGAAAGAAATGATTCGAGCGCATTGCCCTGCACCGGCCTCTTACATTCAGGTACTAGGTTTAAATCTTATTTTGGTCTCTAAACTTTCATGTTTATTGTTTTAGTTCCTTCGAAATGTGTTCCTAAACCTCTTAAAAACCGTTTTAGTCATTATAGTTACTTTTGATAAGTTATTTATGTAAAGTTTATGTGATATAGCATTCGAAATTTGATAAGATTAGATAACTTAGGGACCATGATAGGATTTGAACTTCGAACCTTTCTTCATTGCACGACATCTACGATAGCTTCTAACTTTTTTCTCGAACCAATGTGTTTAGATTTTGCGGTGTGGTCCACCGCCGATGAACAAGGCCATGGCTGCCCACCTTGATGCTCTTGGATATACACCTGAAATGCAGTTCCAATTCTAGTTCATGGTGACTCTTCAATATTTCGTGATTTAAAGATGAGGACTTCAACTTTCATTCTAAAAATTTAGTTGTACGAGTGAGCATCAAAGATGTGAACTATTATGTGTAACTTTTCAAACATGGCTGTGATTTCGCATTACAATTGATGCCAATAAAATGGGAATAATTTCTTGCGTTTGTTGTAATTGTTAACTTCTTGATGGAATAACGCGCGTCTGCTAGGTAGAGGTTTCCATACCCTTGTAAAGAATGCTTCATTCTCCTCCCCAACCGATATGGGTGTTGG

mRNA sequence

CCATTCCTTTGACATTTTCTGGATCTCCATCATCATTTACTTGACCAGGACTCAAATCCAACTCCTTAAACCCACCATTTTTCTTCCTTCTTACCTACCCCTCATTCTTCCAAGTCTCCCAATTTCTGATGGAGATCTTACAGGCTCTCGATGCTCAGGTTCTCACTGGCTTAGCCGCCGCCCTGGTTGCCATTGTTGCTGGTGCCTTTTTCCTTGTCTCCTCCAGAAAACCAGCATCCAGAGCCTGCTTGGATCCTGATAACTTTAAGGAGTTTAAACTTGTTAAGCGCACGAAGCTGAGCCATAATGTGGCAAAGTTCATTTTTATACTTCCCAAGACCGATTCAATTCTTGGTCTTCCTATTGGACAGCATCTAAGTTGCAGGGGAAAGGATAGCCAAGGTGAAGAGGTTATTAAATCTTACACCCCAGTTACCTTGGACTCTGATGTTGGATATTTTGAGCTAGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTTCGAGAAATGCGTATGGGGGACTTTCTTGCTGTGAAGGGACCAAAGGGAAGTTTCAAGTATCAACCTGGTAAAGTTAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACACCCATGTATCAAGTTGCTCGGTCTATATTAGAAAACCCGAACGACAAGACAAAGATTCACCTTATTTATGCCAATGTTACACTTGAAGATATTCTTTTGAAGGAGGAATTGGATCTTCTTGCCAAGAGATACCCTGATCGCTTCAAGATCTACTATGTGTTAAACCAGCCTCCTAAAGTTTGGGATGGCGGCGTCGGCTTTGTGTCGAAAGAAATGATTCGAGCGCATTGCCCTGCACCGGCCTCTTACATTCAGATTTTGCGGTGTGGTCCACCGCCGATGAACAAGGCCATGGCTGCCCACCTTGATGCTCTTGGATATACACCTGAAATGCAGTTCCAATTCTAGTTCATGGTGACTCTTCAATATTTCGTGATTTAAAGATGAGGACTTCAACTTTCATTCTAAAAATTTAGTTGTACGAGTGAGCATCAAAGATGTGAACTATTATGTGTAACTTTTCAAACATGGCTGTGATTTCGCATTACAATTGATGCCAATAAAATGGGAATAATTTCTTGCGTTTGTTGTAATTGTTAACTTCTTGATGGAATAACGCGCGTCTGCTAGGTAGAGGTTTCCATACCCTTGTAAAGAATGCTTCATTCTCCTCCCCAACCGATATGGGTGTTGG

Coding sequence (CDS)

ATGGAGATCTTACAGGCTCTCGATGCTCAGGTTCTCACTGGCTTAGCCGCCGCCCTGGTTGCCATTGTTGCTGGTGCCTTTTTCCTTGTCTCCTCCAGAAAACCAGCATCCAGAGCCTGCTTGGATCCTGATAACTTTAAGGAGTTTAAACTTGTTAAGCGCACGAAGCTGAGCCATAATGTGGCAAAGTTCATTTTTATACTTCCCAAGACCGATTCAATTCTTGGTCTTCCTATTGGACAGCATCTAAGTTGCAGGGGAAAGGATAGCCAAGGTGAAGAGGTTATTAAATCTTACACCCCAGTTACCTTGGACTCTGATGTTGGATATTTTGAGCTAGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTTCGAGAAATGCGTATGGGGGACTTTCTTGCTGTGAAGGGACCAAAGGGAAGTTTCAAGTATCAACCTGGTAAAGTTAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACACCCATGTATCAAGTTGCTCGGTCTATATTAGAAAACCCGAACGACAAGACAAAGATTCACCTTATTTATGCCAATGTTACACTTGAAGATATTCTTTTGAAGGAGGAATTGGATCTTCTTGCCAAGAGATACCCTGATCGCTTCAAGATCTACTATGTGTTAAACCAGCCTCCTAAAGTTTGGGATGGCGGCGTCGGCTTTGTGTCGAAAGAAATGATTCGAGCGCATTGCCCTGCACCGGCCTCTTACATTCAGATTTTGCGGTGTGGTCCACCGCCGATGAACAAGGCCATGGCTGCCCACCTTGATGCTCTTGGATATACACCTGAAATGCAGTTCCAATTCTAG

Protein sequence

MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPNDKTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF

Homology

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BLAST of CmaCh06G000550 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.2e-125
Identity = 219/280 (78.21%), Postives = 247/280 (88.21%), Query Frame = 0


Query: 2   EILQALDAQVLTGLAAALVAIVAG-AFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 61
           E L+ LD Q+L G+  A VA+ AG A+FL SS+K   R CLDP+NFKEFKLVKR +LSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKK--RRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 121
           VAKF+F LP + S+LGLPIGQH+SCRGKD QGE+VIK YTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 181
           GRMSHHFREMR+GD LAVKGPKG FKYQPG+ RAFGMLAGGSGITPM+QVAR+ILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 241
           KTK+HLIYANVT +DILLKEEL+ L   YP++FKI+YVLNQPP+VWDGGVGFVSKEMI+ 
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAA+L+ALGY+PEMQFQF
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of CmaCh06G000550 vs. ExPASy Swiss-Prot
Match: Q6BUX2 (NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.7e-71
Identity = 126/262 (48.09%), Postives = 180/262 (68.70%), Query Frame = 0


Query: 11  VLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHNVAKFIFILPK 70
           ++    AA+++     +F   SRK A    L P++F++F L+++T++SHN   + F LP+
Sbjct: 10  IIFATVAAIISSAVAYYFFQLSRKNA--PVLKPNDFQKFPLIEKTRVSHNTCVYRFGLPR 69

Query: 71  TDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREM 130
           +   LGLPIGQH++  G     +EV++SYTP++ D ++GYF+L+IK Y  G +S H    
Sbjct: 70  STDRLGLPIGQHIAI-GATINDKEVVRSYTPISTDDELGYFDLLIKAYENGNISRHVESK 129

Query: 131 RMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPNDKTKIHLIYAN 190
           ++G+ + ++GPKG F Y PG V +FGM+AGG+GITPMYQ+  +IL NP DKTK+ L+YAN
Sbjct: 130 KIGETIDIRGPKGFFTYTPGMVESFGMIAGGTGITPMYQILTAILRNPEDKTKVSLVYAN 189

Query: 191 VTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYIQ 250
           VT +DILLKEEL+ +A+ +PDRFKIYYVLN PP+ W GGVGFV+ E++  H P  +    
Sbjct: 190 VTEDDILLKEELNKMAREHPDRFKIYYVLNTPPENWTGGVGFVTPEIMDKHLPKASEATN 249

Query: 251 ILRCGPPPMNKAMAAHLDALGY 273
           +L CGPPPM  AM      LGY
Sbjct: 250 LLLCGPPPMISAMKKAAVGLGY 268

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BLAST of CmaCh06G000550 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 268.5 bits (685), Expect = 8.7e-71
Identity = 135/264 (51.14%), Postives = 180/264 (68.18%), Query Frame = 0


Query: 16  AAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSIL 75
           A AL AI+AG        KP  R  L+P  F+EF L ++T +SHNV  + F LP+   IL
Sbjct: 34  AVALTAILAGLKLFTGGSKP--RKVLNPTEFQEFVLKEKTDISHNVCIYRFALPRPADIL 93

Query: 76  GLPIGQHLSCRGK-DSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGD 135
           GLPIGQH+S     + Q +EV++SYTP++ D++ GYF+L++K YPQG +S +   +++GD
Sbjct: 94  GLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIGD 153

Query: 136 FLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILEN-----PNDKTKIHLIYA 195
            + V+GPKG+  Y P   R  GM+AGG+GITPM Q+ ++++ N      ND TK+ LI+A
Sbjct: 154 TMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIFA 213

Query: 196 NVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYI 255
           NV  +DILLKEELD LA   PD F IYYVLN PP+ W GGVGFV+ EMI+ H PAPAS +
Sbjct: 214 NVNPDDILLKEELDKLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASDV 273

Query: 256 QILRCGPPPMNKAMAAHLDALGYT 274
           +IL CGPPPM  AM    ++LGYT
Sbjct: 274 KILLCGPPPMISAMKKATESLGYT 294

[Close]

BLAST of CmaCh06G000550 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-70
Identity = 133/264 (50.38%), Postives = 180/264 (68.18%), Query Frame = 0


Query: 16  AAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSIL 75
           A A+ AI+AG        KP  R  L+P  F+EF L ++  +SHNV  + F LP+   IL
Sbjct: 34  AVAVTAILAGLKLFTGGNKP--RKVLNPTEFQEFVLKEKIDVSHNVCIYRFALPRPTDIL 93

Query: 76  GLPIGQHLSCRGK-DSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGD 135
           GLPIGQH+S     + Q +EV++SYTP++ D++ GYF+L++K YPQG +S +   +++GD
Sbjct: 94  GLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIGD 153

Query: 136 FLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILEN-----PNDKTKIHLIYA 195
            + V+GPKG+  Y P   R  GM+AGG+GITPM Q+ ++++ N      ND TK+ LI+A
Sbjct: 154 TMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIFA 213

Query: 196 NVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYI 255
           NV  +DILLKEELD+LA   PD F IYYVLN PP+ W GGVGFV+ EMI+ H PAPAS +
Sbjct: 214 NVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASDV 273

Query: 256 QILRCGPPPMNKAMAAHLDALGYT 274
           +IL CGPPPM  AM    ++LGYT
Sbjct: 274 KILLCGPPPMISAMKKATESLGYT 294

[Close]

BLAST of CmaCh06G000550 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.3e-70
Identity = 125/240 (52.08%), Postives = 172/240 (71.67%), Query Frame = 0


Query: 41  LDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYT 100
           LDP  +++F+L ++  ++HN   F F LP  D ILGLPIGQH+S R     G+EV + YT
Sbjct: 49  LDPQEYRKFQLKEKFIVNHNTRIFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYT 108

Query: 101 PVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAG 160
           P++ D + GYF+L+IK+Y +G MS +   M +GD + VKGPKG F YQP   ++ GMLAG
Sbjct: 109 PISSDEERGYFDLLIKVYEKGAMSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAG 168

Query: 161 GSGITPMYQVARSILENPNDKTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLN 220
           G+GITPM QV ++IL+NP+DKT+I L++ N+T EDILLK+ELD LA+++P +FK+YYVLN
Sbjct: 169 GTGITPMLQVIKAILKNPSDKTEISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLN 228

Query: 221 QPPKVWDGGVGFVSKEMIRAHCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280
            PPK W  GVGFVSKE+I +  P+P+    ++ CGPP MNKAM  HL+ +G+     F F
Sbjct: 229 NPPKGWTQGVGFVSKEIIESRLPSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

[Close]

BLAST of CmaCh06G000550 vs. ExPASy TrEMBL
Match: A0A6J1I558 (NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111470015 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 7.4e-158
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. ExPASy TrEMBL
Match: A0A6J1FHS1 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111445460 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 1.8e-151
Identity = 270/280 (96.43%), Postives = 275/280 (98.21%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQALDAQVL GLAAA+VAIVAGAFFLVSSRKPAS ACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLVGLAAAVVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPG+VRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. ExPASy TrEMBL
Match: A0A1S3B6Z6 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103486847 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.0e-143
Identity = 253/280 (90.36%), Postives = 265/280 (94.64%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           ME+LQALD+QVL GL AALVAIV GA FL SSRKPASRACLDP+ FKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGD LAVKGPKG FKYQPG+V AFGMLAGGSGITPMYQV+R+ILENP D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTK+HLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPA+ I+ILRCGPPPMNKAM  HLDALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. ExPASy TrEMBL
Match: A0A5D3DPB8 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006230 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.0e-143
Identity = 253/280 (90.36%), Postives = 265/280 (94.64%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           ME+LQALD+QVL GL AALVAIV GA FL SSRKPASRACLDP+ FKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGD LAVKGPKG FKYQPG+V AFGMLAGGSGITPMYQV+R+ILENP D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTK+HLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPA+ I+ILRCGPPPMNKAM  HLDALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. ExPASy TrEMBL
Match: E5GBY3 (NADH-cytochrome b5 reductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.0e-143
Identity = 253/280 (90.36%), Postives = 265/280 (94.64%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           ME+LQALD+QVL GL AALVAIV GA FL SSRKPASRACLDP+ FKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGD LAVKGPKG FKYQPG+V AFGMLAGGSGITPMYQV+R+ILENP D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTK+HLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPA+ I+ILRCGPPPMNKAM  HLDALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. NCBI nr
Match: XP_022971225.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita maxima])

HSP 1 Score: 566.2 bits (1458), Expect = 1.5e-157
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. NCBI nr
Match: XP_023540168.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 550.4 bits (1417), Expect = 8.7e-153
Identity = 273/280 (97.50%), Postives = 275/280 (98.21%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQALDAQVL GLA ALVAIVAGAFFLVSSRK ASRACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLIGLATALVAIVAGAFFLVSSRKSASRACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPG+VRAFGMLAGGSGITPMYQVARSILENPND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. NCBI nr
Match: KAG7027734.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.7 bits (1410), Expect = 5.7e-152
Identity = 272/280 (97.14%), Postives = 275/280 (98.21%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQALDAQVL GLAAALVAIVAGAFFLVSSRKPAS ACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. NCBI nr
Match: KAG6596195.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 546.2 bits (1406), Expect = 1.6e-151
Identity = 271/280 (96.79%), Postives = 274/280 (97.86%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQA DAQVL GLAAALVAIVAGAFFLVSSRKPAS ACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQAFDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. NCBI nr
Match: XP_022939629.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita moschata])

HSP 1 Score: 545.0 bits (1403), Expect = 3.7e-151
Identity = 270/280 (96.43%), Postives = 275/280 (98.21%), Query Frame = 0


Query: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60
           MEILQALDAQVL GLAAA+VAIVAGAFFLVSSRKPAS ACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLVGLAAAVVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPG+VRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of CmaCh06G000550 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 450.7 bits (1158), Expect = 8.8e-127
Identity = 219/280 (78.21%), Postives = 247/280 (88.21%), Query Frame = 0


Query: 2   EILQALDAQVLTGLAAALVAIVAG-AFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 61
           E L+ LD Q+L G+  A VA+ AG A+FL SS+K   R CLDP+NFKEFKLVKR +LSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKK--RRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 121
           VAKF+F LP + S+LGLPIGQH+SCRGKD QGE+VIK YTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 181
           GRMSHHFREMR+GD LAVKGPKG FKYQPG+ RAFGMLAGGSGITPM+QVAR+ILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 241
           KTK+HLIYANVT +DILLKEEL+ L   YP++FKI+YVLNQPP+VWDGGVGFVSKEMI+ 
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAA+L+ALGY+PEMQFQF
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of CmaCh06G000550 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 205.7 bits (522), Expect = 4.9e-53
Identity = 112/261 (42.91%), Postives = 157/261 (60.15%), Query Frame = 0


Query: 38  RACLDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSILGLPIGQHLSCR---GKDSQGEE 97
           +  L+PD + EFKL    ++SHN   F F    + + LGL +   L  R   G +++G+ 
Sbjct: 70  KTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPS-AELGLHVASCLLTRAPLGYNAEGKT 129

Query: 98  --VIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGDFLAVKGPKGSFKYQPGKV 157
             VI+ YTP++     GYF+L+IK+YP G+MS HF  ++ GD L VKGP   FKY P   
Sbjct: 130 KYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKGPVEKFKYSPNMK 189

Query: 158 RAFGMLAGGSGITPMYQVARSILENPNDKTKIHLIYANVTLEDILLKEELDLLAKRYPDR 217
           +  GM+AGGSGITPM QV  +I++NP D T+I L+YANV+ +DILLK++LD+L   +P+ 
Sbjct: 190 KHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQKLDVLQANHPN- 249

Query: 218 FKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYIQILRCGPPPMNKAMAAH------ 277
            KI+Y ++ P K W GGVG++SK+M     P P     IL CGPP M + ++        
Sbjct: 250 LKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMMEHISGGKAPDWS 309

Query: 278 -------LDALGYTPEMQFQF 281
                  L  LGYT EM F+F
Sbjct: 310 QGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of CmaCh06G000550 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 177.2 bits (448), Expect = 1.9e-44
Identity = 99/261 (37.93%), Postives = 156/261 (59.77%), Query Frame = 0


Query: 39  ACLDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKS 98
           A ++P      +LV++T +SH+V KF F LP  D +LGLP+G+H+      +  +  +++
Sbjct: 655 ALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRA 714

Query: 99  YTPVTLDSDVGYFELVIKMY-----PQ----GRMSHHFREMRMGDFLAVKGPKGSFKY-- 158
           YTP +    VGYFELV+K+Y     P+    G MS +   + +G  L +KGP G  +Y  
Sbjct: 715 YTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLG 774

Query: 159 --------QPGKVRAFGMLAGGSGITPMYQVARSILENPNDKTKIHLIYANVTLEDILLK 218
                   +P       MLAGG+GITP+YQ+ ++IL++P D+T++++IYAN T EDILL+
Sbjct: 775 KGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLR 834

Query: 219 EELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYIQI-LRCGPPP 278
           EELD  A++YPDR K++YV+    + W    GF+S+ ++R H P       + + CGPPP
Sbjct: 835 EELDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPP 894

[Close]

BLAST of CmaCh06G000550 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 169.1 bits (427), Expect = 5.1e-42
Identity = 90/255 (35.29%), Postives = 151/255 (59.22%), Query Frame = 0


Query: 39  ACLDPDNFKEFKLVKRTKLSHNVAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKS 98
           A ++P      +L+++T +SH+V KF F LP  D  LGLP+G+H+     +   +  +++
Sbjct: 655 ALVNPREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCA-NINDKLCLRA 714

Query: 99  YTPVTLDSDVGYFELVIKMY-----PQ----GRMSHHFREMRMGDFLAVKGPKGSFKY-- 158
           YTP +    VG+ +LV+K+Y     P+    G MS H   + +G  + +KGP G  +Y  
Sbjct: 715 YTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKG 774

Query: 159 --------QPGKVRAFGMLAGGSGITPMYQVARSILENPNDKTKIHLIYANVTLEDILLK 218
                   +P   +   MLAGG+GITP+YQ+ +SIL +P D+T+++++YAN T +DIL++
Sbjct: 775 KGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVR 834

Query: 219 EELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASYIQI-LRCGPPP 273
           EEL+  A ++ +R KI+YV+    + W    GF+++ ++R H P       + L CGPPP
Sbjct: 835 EELEGWASKHKERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPP 894

The following BLAST results are available for this feature:

BLAST of CmaCh06G000550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	1.2e-125	78.21	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
Q6BUX2	8.7e-71	48.09	NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS...	[more]
A1DHW1	8.7e-71	51.14	NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3...	[more]
Q4X0B5	2.5e-70	50.38	NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A...	[more]
Q54NC1	3.3e-70	52.08	NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE...	[more]

BLAST of CmaCh06G000550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I558	7.4e-158	100.00	NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111470015 PE=3 SV...	[more]
A0A6J1FHS1	1.8e-151	96.43	NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111445460 PE=3 ...	[more]
A0A1S3B6Z6	1.0e-143	90.36	NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103486847 PE=3 SV=1	[more]
A0A5D3DPB8	1.0e-143	90.36	NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GBY3	1.0e-143	90.36	NADH-cytochrome b5 reductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

BLAST of CmaCh06G000550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022971225.1	1.5e-157	100.00	NADH--cytochrome b5 reductase 1-like [Cucurbita maxima]	[more]
XP_023540168.1	8.7e-153	97.50	NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7027734.1	5.7e-152	97.14	NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6596195.1	1.6e-151	96.79	NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022939629.1	3.7e-151	96.43	NADH--cytochrome b5 reductase 1-like [Cucurbita moschata]	[more]

BLAST of CmaCh06G000550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	8.8e-127	78.21	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	4.9e-53	42.91	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	1.9e-44	37.93	nitrate reductase 2	[more]
AT1G77760.1	5.1e-42	35.29	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 194..205 score: 43.58 coord: 156..175 score: 37.3 coord: 250..258 score: 37.16
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 194..205 score: 64.06 coord: 156..175 score: 63.33 coord: 250..258 score: 59.49 coord: 133..147 score: 49.17 coord: 97..104 score: 41.93 coord: 75..86 score: 56.08
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 49..147 e-value: 8.6E-31 score: 106.2
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 38..137 e-value: 1.5E-32 score: 114.2
None	No IPR available	PANTHER	PTHR19370:SF203	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..280
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..280
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 49..280 e-value: 6.59605E-116 score: 330.683
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 138..280 e-value: 1.2E-43 score: 150.3
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 136..276
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 157..263 e-value: 1.9E-29 score: 102.5
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 44..148 score: 13.022097
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 41..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G000550.1	CmaCh06G000550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity