CmaCh06G000540 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G000540 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 532.3 bits (1370), Expect = 5.4e-150
Identity = 276/466 (59.23%), Postives = 341/466 (73.18%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSS C+S CY  + D  +   D           A  G      +C KCK    M+  
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDD----------AVSGGGNHESVCVKCKCNAPMTFG 60

Query: 61  EEG-DDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQV 120
           + G DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG  SRV+LQFVHE+Q+
Sbjct: 61  DGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQI 120

Query: 121 KAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHI 180
           KA KN+EASRDRSL VFGVGVA++DET+A P    E+   I+ ++  VS LSPP KDLH+
Sbjct: 121 KALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHV 180

Query: 181 VQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTC 240
           V +ES++ S+  D R++L KLLD+V D TGKEDL+ HL+MLSLQK+AAENGYNRLVLG+C
Sbjct: 181 VPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSC 240

Query: 241 TSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVE 300
           TSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLRDC+  E+  LC  DGLKTVE
Sbjct: 241 TSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVE 300

Query: 301 LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK 360
           L   S  GINDLVSSFVALLQEENPSRE TIVRTA KLTPF F++IP+ ++SN VP+AT+
Sbjct: 301 LACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSN-VPMATQ 360

Query: 361 RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL 420
           RR KR N K+  S+++E FC +CNGPLN SD   L +FE GQ S +L   CCSSC+FQIL
Sbjct: 361 RRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCSSCRFQIL 420

Query: 421 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDE 466
           P+D  S E   S LP  M+++  H    +   LREKI++ LL DDE
Sbjct: 421 PQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

BLAST of CmaCh06G000540 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.1e-105
Identity = 211/466 (45.28%), Postives = 291/466 (62.45%), Query Frame = 0

Query: 2   ACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSSE 61
           +C  + C  +C  +    A  +  +    K  RL+        +  C KC       +  
Sbjct: 8   SCGGAGCGPHCSSSASAAAVEDAAAAAAEKVGRLSL-------SRECGKCGGGAAAVAVA 67

Query: 62  EGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKA 121
            G     C +CFR+NL  KF+ AV SNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA
Sbjct: 68  GGLG--LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKA 127

Query: 122 HKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQ 181
            ++++ S  ++L VFGVGVA++DE+  C  P  EI+  I+ I+S+VSSLS  +K +HI +
Sbjct: 128 IESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIAR 187

Query: 182 IESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTS 241
           +E V+S+   DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY +++LG+C S
Sbjct: 188 LEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCAS 247

Query: 242 RIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVELT 301
            IACHV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDCLAQEL +LC  D LKT +  
Sbjct: 248 AIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHL 307

Query: 302 KTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRR 361
               +GIN LV+SF+  L+EENPSRE TIVRTA KL PF+F++       + +P  ++ R
Sbjct: 308 DRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLR 367

Query: 362 QKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRS--FEAGQVSSILAGTCCSSCQFQIL 421
            K   F    S  SE  C +C  P + S++Q L S   +A +   +    CC SC FQIL
Sbjct: 368 PKFQKFDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQIL 427

Query: 422 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDE 466
           P      E   SLLP     +    S S+S  LR++I+E+LL +++
Sbjct: 428 PAGENLNEHFFSLLPKLWTGKMDTISDSHS-LLRDQIEEYLLEEND 461

BLAST of CmaCh06G000540 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.9e-105
Identity = 211/466 (45.28%), Postives = 291/466 (62.45%), Query Frame = 0

Query: 2   ACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSSE 61
           +C  + C  +C  +    A  +  +    K  RL+        +  C KC       +  
Sbjct: 8   SCGGAGCGPHCSSSASAAAVEDAAAAAAEKVGRLSL-------SRECGKCGGGAAAVAVA 67

Query: 62  EGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKA 121
            G     C +CFR+NL  KF+ AV SNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA
Sbjct: 68  GGLG--LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKA 127

Query: 122 HKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQ 181
            ++++ S  ++L VFGVGVA++DE+  C  P  EI+  I+ I+S+VSSLS  +K +HIV+
Sbjct: 128 IESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIVR 187

Query: 182 IESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTS 241
           +E V+S+   DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY +++LG+C S
Sbjct: 188 LEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCAS 247

Query: 242 RIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVELT 301
            IACHV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDCLAQEL +LC  D LKT +  
Sbjct: 248 AIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHL 307

Query: 302 KTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRR 361
               +GIN LV+SF+  L+EENPSRE TIVRTA KL PF+F++       + +P  ++ R
Sbjct: 308 DRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLR 367

Query: 362 QKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRS--FEAGQVSSILAGTCCSSCQFQIL 421
            K        S  SE  C +C  P + S++Q L S   +A +   +    CC SC FQIL
Sbjct: 368 PKFQKVDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQIL 427

Query: 422 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDE 466
           P      E   SLLP     +    S S+S  LR++I+E+LL +++
Sbjct: 428 PAGENLNEHFFSLLPKLWTGKMDTISDSHS-LLRDQIEEYLLEEND 461

BLAST of CmaCh06G000540 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.3e-22
Identity = 132/516 (25.58%), Postives = 208/516 (40.31%), Query Frame = 0

Query: 47  LCFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPC 106
           +C KCK          GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 107 SRVALQFVHEMQVKAHKNFEASRDRSLHV-FGVGVAYIDETSACPIPLHEIDRTIQTIQS 166
           S   ++ V E           SRD    + F  G+ +IDE +AC +   E  + +  I S
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQQILSEICS 143

Query: 167 VVSSLSPPIKDLHIVQIESVYS---SNLSDG----------------------------- 226
           V+     P    HIV +E V+S   S L  G                             
Sbjct: 144 VLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 227 -------------------------------REKLKKLLDTVSDVTGKEDLISHLRMLSL 286
                                           +KLK+L  +   +T K+ L+  LR   +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 287 QKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLRDC 346
             IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R+ 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 347 LAQELNMLCCADGLKTVEL----TKTS-FSGINDLVSSFVALLQEENPSRESTIVRTAGK 406
            ++E+        +  + +    TK S  S I  L   FV  LQ + PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 407 LTPFNFSRI----------------PDLNESNGVPLATKRRQKRNNFKH-----VNSLSS 466
           L   N S+I                  L+   G   A    Q  ++         N L++
Sbjct: 384 L---NVSKIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLAN 443

Query: 467 ETFCAVCNGPLNMSDV---QTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSL 469
            +  + C G     +     T +S  A Q  S +    C SC+  +  KD  S    L +
Sbjct: 444 NSDKSCCQGGQGCKEAGYGDTCQS-RALQTPSFV-HMLCYSCRLTV--KDMQS----LDV 503

BLAST of CmaCh06G000540 vs. ExPASy Swiss-Prot
Match: Q28ES8 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Xenopus tropicalis OX=8364 GN=ctu2 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.1e-20
Identity = 132/530 (24.91%), Postives = 199/530 (37.55%), Query Frame = 0

Query: 48  CFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCS 107
           C KCK    +     GD   FC  CF+     KFR  +  N +I P +KVL+AFSGGP S
Sbjct: 25  CMKCKESSAVLLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAFSGGPSS 84

Query: 108 RVALQFVHEMQVKAHKNFEASRDRSLHV-FGVGVAYIDETSACPIPLHEIDRTIQTIQSV 167
              +Q V E           SRD    + F  G+ +IDE + C +   E  + +  +Q+V
Sbjct: 85  SAMVQQVQE---------GMSRDAPKKLRFVPGILFIDEGTTCGMSHEERQQNMSEVQNV 144

Query: 168 VSSLSPPIKDLHIVQIESVYS---------------------------------SNLSDG 227
           +     P    +IV +E V+S                                 +  SD 
Sbjct: 145 LQQTGFP---FYIVSLEQVFSLPGSILQRGVPEQRANYKQEVDRFMVQQQAQGETGCSDI 204

Query: 228 REKLKKLLDTVSD---------------------------------VTGKEDLISHLRML 287
            E L KL   VS+                                 +T K  L+  LR  
Sbjct: 205 LENLAKLGVNVSESSRESDKMFQSTCRHPPDTHTQKLIQLFASAKTLTAKHQLLHTLRSH 264

Query: 288 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLR 347
            +  IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R
Sbjct: 265 LILHIARTCGYSKVMSGESCTRLSVSLLSNISLGRGAFLPLDTGFCDSRYGDVDIIRPMR 324

Query: 348 DCLAQEL---NMLCCADG--LKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTA 407
           +   +E+   N L       +  +       S I  L   F+  LQ + PS  ST+ RT+
Sbjct: 325 EYSLKEIAFYNRLFHVSSVFIPALNTKVLENSSIQQLSEVFINRLQADFPSTVSTVYRTS 384

Query: 408 GKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTL 466
            KL   N S+I D N+        K R                 C +C  PL+ + V   
Sbjct: 385 EKL---NVSKI-DANQGT----CAKER-----------------CLLCLSPLD-TQVGEA 444

BLAST of CmaCh06G000540 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.1e-265
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180
           AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300

Query: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

BLAST of CmaCh06G000540 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 1.6e-261
Identity = 463/468 (98.93%), Postives = 464/468 (99.15%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSSACQSNCYRNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180
           AHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKT EL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEEL 300

Query: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLRE+IQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of CmaCh06G000540 vs. ExPASy TrEMBL
Match: A0A6J1DEW9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111019863 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.7e-219
Identity = 398/476 (83.61%), Postives = 423/476 (88.87%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60
           MACNSS CQSNCYRNQDDE T+EQ S   NK +  AA       T GD G+THLCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60

Query: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180
           VHE+QVKAHKNFEASRDRSL VFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180

Query: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240

Query: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300

Query: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360
           GLKTVEL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360

Query: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 420
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420

Query: 421 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLRE+IQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 475

BLAST of CmaCh06G000540 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.0e-215
Identity = 392/475 (82.53%), Postives = 418/475 (88.00%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60
           MACNSSACQSNCYRNQ DE T+EQDS  +++ D  AA       T  D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180
           VHEMQVKAH NFEASRDRSL VFGVGVAYI+ET+AC IP  EID+T+QTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNL+DGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300
           L++GTCTSRIACHVISSTVKGQGYSLP D+QY+DARWEVPV LPL DCLAQELNMLCC D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300

Query: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360
           GLKTVELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDV T  +       S + G CCSS
Sbjct: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHA------GSSILGACCSS 420

Query: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           CQFQILPKDS SQEL LSLLPPSMVARA HGS  NSNSLR+KIQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

BLAST of CmaCh06G000540 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 5.0e-215
Identity = 392/473 (82.88%), Postives = 416/473 (87.95%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-----TYGDSGDTHLCFKCKAKE 60
           MACNSSACQSNCYRNQ DE T+EQDS  +++    A      T  D+ +T+LCFKCK+KE
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESRITNDDNSNTNLCFKCKSKE 60

Query: 61  RMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVH 120
           RMS+S  GDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQFVH
Sbjct: 61  RMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVH 120

Query: 121 EMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIK 180
           E+QVKAHKNFEASRDRSL VFGVGVAYI+ET+A  IP  EID+TIQTIQSVVSSLS P K
Sbjct: 121 EIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQPRK 180

Query: 181 DLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLV 240
           DLHIV IE+VYSSNLSDGREKLKKLLD V DVTGKEDLIS+LRMLSLQKIAAENGYNRL+
Sbjct: 181 DLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNRLL 240

Query: 241 LGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGL 300
           +GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLCC DGL
Sbjct: 241 VGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCIDGL 300

Query: 301 KTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVP 360
           KTVELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN VP
Sbjct: 301 KTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVP 360

Query: 361 LATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQ 420
           LATKRRQKRNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS + G CCSSCQ
Sbjct: 361 LATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSILGACCSSCQ 420

Query: 421 FQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           FQILPKDS+S EL LSLLPPSMVARA HGS  NSNSLR KIQEFLLSDD+ ES
Sbjct: 421 FQILPKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

BLAST of CmaCh06G000540 vs. NCBI nr
Match: XP_022971223.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima])

HSP 1 Score: 923.7 bits (2386), Expect = 6.3e-265
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180
           AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300

Query: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

BLAST of CmaCh06G000540 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 911.4 bits (2354), Expect = 3.3e-261
Identity = 463/468 (98.93%), Postives = 464/468 (99.15%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSSACQSNCYRNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180
           AHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKT EL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEEL 300

Query: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLRE+IQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of CmaCh06G000540 vs. NCBI nr
Match: XP_023539180.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 910.2 bits (2351), Expect = 7.3e-261
Identity = 462/468 (98.72%), Postives = 463/468 (98.93%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSS CQSNCYRNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180
           AHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTIQ IQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQSVVSSLSPPIKDLHIV 180

Query: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300

Query: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLRE+IQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of CmaCh06G000540 vs. NCBI nr
Match: KAG7027733.1 (Cytoplasmic tRNA 2-thiolation protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 896.7 bits (2316), Expect = 8.3e-257
Identity = 459/469 (97.87%), Postives = 461/469 (98.29%), Query Frame = 0

Query: 5   SSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSSEEGD 64
           SSACQSNC+RNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSSEEGD
Sbjct: 17  SSACQSNCHRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSEEGD 76

Query: 65  DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHKN 124
           DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHKN
Sbjct: 77  DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHKN 136

Query: 125 FEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQIES 184
           FEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQIES
Sbjct: 137 FEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQIES 196

Query: 185 VYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTSRIA 244
           VYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTSRIA
Sbjct: 197 VYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTSRIA 256

Query: 245 CHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADG-----LKTVE 304
           CHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADG     LKTVE
Sbjct: 257 CHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGSVFGSLKTVE 316

Query: 305 LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK 364
           LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK
Sbjct: 317 LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK 376

Query: 365 RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL 424
           RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL
Sbjct: 377 RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL 436

Query: 425 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLRE+IQEFLLSDDEVES
Sbjct: 437 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 485

BLAST of CmaCh06G000540 vs. NCBI nr
Match: KAG6596194.1 (Cytoplasmic tRNA 2-thiolation protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 833.6 bits (2152), Expect = 8.6e-238
Identity = 431/468 (92.09%), Postives = 434/468 (92.74%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSSACQSNC+RNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCHRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180
           AHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300

Query: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360
           TKTSFS INDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNE          
Sbjct: 301 TKTSFSSINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNE---------- 360

Query: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420
                               +CNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 --------------------ICNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 469
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLRE+IQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 438

BLAST of CmaCh06G000540 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 532.3 bits (1370), Expect = 3.8e-151
Identity = 276/466 (59.23%), Postives = 341/466 (73.18%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60
           MACNSS C+S CY  + D  +   D           A  G      +C KCK    M+  
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDD----------AVSGGGNHESVCVKCKCNAPMTFG 60

Query: 61  EEG-DDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQV 120
           + G DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG  SRV+LQFVHE+Q+
Sbjct: 61  DGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQI 120

Query: 121 KAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHI 180
           KA KN+EASRDRSL VFGVGVA++DET+A P    E+   I+ ++  VS LSPP KDLH+
Sbjct: 121 KALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHV 180

Query: 181 VQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTC 240
           V +ES++ S+  D R++L KLLD+V D TGKEDL+ HL+MLSLQK+AAENGYNRLVLG+C
Sbjct: 181 VPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSC 240

Query: 241 TSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVE 300
           TSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLRDC+  E+  LC  DGLKTVE
Sbjct: 241 TSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVE 300

Query: 301 LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK 360
           L   S  GINDLVSSFVALLQEENPSRE TIVRTA KLTPF F++IP+ ++SN VP+AT+
Sbjct: 301 LACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSN-VPMATQ 360

Query: 361 RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL 420
           RR KR N K+  S+++E FC +CNGPLN SD   L +FE GQ S +L   CCSSC+FQIL
Sbjct: 361 RRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCSSCRFQIL 420

Query: 421 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDE 466
           P+D  S E   S LP  M+++  H    +   LREKI++ LL DDE
Sbjct: 421 PQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455