CmaCh05G011570 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh05G011570
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	branched-chain-amino-acid aminotransferase-like protein 1
Location	Cma_Chr05: 8992019 .. 8997740 (-)
RNA-Seq Expression	CmaCh05G011570
Synteny	CmaCh05G011570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCTCGCCACTTTCCAGTTGCCATTGCGATTTGATCGCTGCGGCTCGCACTGTCTGTACAATCCGACCACCAACCATGGCGGAGGAGCTCGGCATCGACGTCTCTGTTCCTCCTAGCCTCCATCTCATCTCTGCTTTCCTTGCCATGGAGCCAGCTGATTCTTTACTTTGCATGGCCAGGTACCATTCTAATACCCCCACTTTAACTTCAGCTGAAATGCTCTCATACTAACGCGCTTTCTATCTCTGTGCCCACTTTATTGGAGTTTTATATCATTTCCCGAATGGATTACACATTTCTTCTCGATTCGTTTATTTGCGTTTCTCATAATGGAGGTGGATAATGTTTTTTTTGTCTCTTTTAGGGAATTGGGTCGCGGATCAGTGACCGAGACAGTGCAGAGGTTCATTTGGGATCATTGCATTAGCAAAGCTGTAGAGATTCTAAACCATTTGATCTCCATTTCATTTTTTTAATTGAATTCAATATAATTTCTCTGCTTGCTTATTAGTTCATGTAGGAATTAAATTTTGTTGGTTCCCTGGAGAGTACACAGAATGTAGATGTTATTGGCATTTGTTGAACTGGATTGGTATTTGAGTTTACTTATTTCTCTGTTTCCTACTTTTTGTCAGCAAGAAATCAACCATTTTCATGTACCATATCTGAAGAATTTTCTGAAGAAGCTGATATCTGAAGTTGAATCAAGTCAAGTTGAAGTGTTAGATGAGTTCTATGAATTGTATGCTCATTACATGGTTTCATGGAAGGTAAGCTGCTGAAGAAGGAAAAACTATTTCATTGATTCATTTGCACATGAGAATCCCTTGGCTATTTTCCTTGCTGTTCTTGAATGATAAGAGTTCTTTTTTGATAGGATGAGAATCTGGGCAAAGAAAGTGCAACAATCTCCAAATTTGTTTCTTTTCTTTTTCCACCTGGCAAGTATTTTCTTCATTTTTGTACTATCTCGTGACTGAAGTAGTCGATAATTTGGTTTTGCTTCCTTGCTTACAAATATTGAATTAATTGGTTCTTTTAGGTTCTTCAAGTTGTCAAAAGTTAAGGAAATTTGTGGTGCCACTACAATGCTCTCTCAATATGCTTGCAGGTGATACAGGGAATGCTTCACACTCTCAACTTTGTTTAAGTTATCTTATGTTCATCATGTGTTAGCATAATAATTTGTGGAAGATTTTGATCTTTAGAGTTTCTGGTTATTGTTCATCTGATTTACGTTTTTCTCTTCGAGCGTTGTGTTCATATATCGATATACTTGGAACATTGTTTTACCATCTGGATTGATGTGCATTCGCTTGAAGTTAACTGCTAAACATAATGGATTTTATGCATTCCTTCTCTGTTGTGGCTGGTATCCAGATTTCCATCTTTATTGTTCTCGGGTATTGCCCAATTTTCTTTTTATTGCTGATGGGAATTTTGTTCTTTCATTCTTCTTAATGAAAGTGGTTCTTTCTCTATATAAATAAAGTACATTGTTGGTTAAAGTCTTATTTTTTGTGAGATCCCACGTTGGTTGGGGAGGATAACAAAACATTATTTATAAGGGTGTGGAAACCTCTCCCTAGCAAACGTGTTTTAAAAACCTTGATAAGAATCCCGAAAGGGAAAACCCAAAAAGGATATAATATTTGCTAGCGGTGGGCTTGGGCTGCTACATTTTTAGTCCTTAACCTTTCAGGTGATTTTATTTTAGTCTCTAAACTTTCAAAATGTGTCGGTGGAATCTAATTTTAGTCCCTAAAATTGAGATTCCACCCTTTATTATTCCGGGGTCTTCCCCTTTCTTTTATTGCTGATGAGGCTTTTGTTCTTTCATTCTTCTTAATGAAAGTGGTTCTTATTTTAAAAAATAAGAAAAAGAAAGAGAGTAAGTATTTAAGTTAAAATAATCTTGCAAAGTTGTTTCAAGTCTGTAGGTCTTTGTATGATACAGGTGTTCACTTTGGCCTTCGAGTCTATACCTTTCGGAACTGATACTCTCGTTTCCAAACATTTTCTCAACAAGAAAATGCTTTGAGGTAAATTTGTACATTTAGTTCTGCCTTAAATCTAGTCCAAAAACTCCTTACATTTGCTATCTGAATCATTGTATACCTCGATGGTCGGTCATTATACTCAAATATTTTGCTTATTATTCCGATCATACGTTGTTAGGAGAAACATACCCTTTTGATGATCTAAGCCTTTTCATCTTTATCTACTTAATTTTTTTTGTTAATAAAAAGAAATAAGAGACGAGTATTATCTAAGCCTGCGCGTTTACACTGGCTATTCTGTGTAAGCTCAGGTGATGTTTGGCTCACAGTTTTAGTAAGTTTGAGCTTATGAAATTTGGGATTAGGAGAGTTTATGAATTGTTAAAGCTAATGAGTATAAAATATTAGTTTCATAATTTGGAATATGTAACCTAATGACTTTTTAACTTAGGTTACACAAACATAACCTTTGATCCGAACGCCTTGGAAGATTTCCATAATTTTCTAGCTATGTTTTACATGAAAACAGGTTGGTTCGGGTGTGGGTTTGGTTGGAATCTGCCTTGCTCGTGTCAAAACGTCATCGGTAAGTTGCTGGAAAACCTTGAAAGCTTGCATTGTTCCCATCAATGGGGACTTTTCAACTTTACCACAAAGTTGACAACCCTGTGTTTTAAACAGATAGTGTTGAGTGATGGTGACCTATCAACATTAGCAAATATGAAGGTTAATTTAAGGTTGAATGGGTTGTGCAGCCTCAGCTCTGCAACTGCTACACCAGAAAGAACTAAAGAGGATCTTCAAACTGTAAGGGCACAATTATTAGGAATATTATACATGTTTTTAGCTATCTGTAGACCTTAATCTCTGCATGCTTACTATTGAAATGGAAGCAAAATGTTTGAAAGTCATTGCGCTTCTGTACGTCTTCCTTCTTTTAAGACCATTCTTAAGCTTTTTCCTCCCTTCCTTTGATTGAGATCCCATGTCGGTTGGAGAGGGGAACGAAACATTTCTTGTAAGGGTGTGGGGACCTCTTCCTAATAGAGTCGTTTTAAAATCGTGAGGCTGATTGTGATACGTAACGGGCCAAAACAGACAATGTCTACTAACGGTGGGTATGAACTGTTTCAAATGGTATCAGAGCCAGACACCGGGCGATGTGCCAACGAGGATGTTGGGCTCCCAAGGGGTGGATTGTGAGATTACACATCGGTTGGAGAGGGGATCAAAACATTCCTTATAAGGGTGTGGAAACCTCTCCTTGATAGATGCATTTTAAAACCGTGAGGTTGACGGTGATACGTAACGGGGCAAAGCGGACAATATCTACTAGCGGTGGACATGAGTTGTTACAAATGGTATCAGAGCCAGACACCGAGTGGTGTACCAGTGAGGATGGTGGGCTCCCAAGGGGGGAGTGAATTGTGAGATCCTACATTGGTTGTAAAGGGGAACGAAACATCCCTTATAAGGGTGTGGAAATCTCTTCCTAACAGACGCATTTTAAAACTGTGAGACTAACGACGATACGTAATGGGCCAAAACAGACAATATCTACTAGCGGTGGGCATGAGCTATTATAAATGGCATTAGAGCCAGACACCGGGCGGTGTGCCAGTGAGGACGTTGGGCTCCCAAGGGAGTGGATTGTGAGATCCCACATTGGTTAAAGAGGAGAACAAAACATTCCTTATAAGGGTGTGAAAACTTCTCCCTAATAGATGCATTTTAAAACCGCAAGACTGACGGTGATACGTAACGGGCTAAATCAGACAATATCTACTATCTGTGGATATGGTCCGTTACAAACTTATTATAAAATCTGTTAGAGTGTGTGAGACTTTCATGTATCAGTTTATAATGAGAACAATATGAATCTCAGGTGGAATGCATTCATCTGCCGTGGGAATCAGCATCAGAAAGTGAACTCCAAGCTTTTGCACCAGATATTGTGTGAGTTTCTACTTTACGATTTGATGTACCTTTCTTTTTGTACAAAATGTCGTATATTTGGTCATCGGTTAAGTAACTCTCCTTTAACATTACAACGTACATCGACAATGACTCTTCTGCTTAGAAATGAAATGTTCTCCGATCCTTGTCATGTAGGTTAGGCGCAGATGTAATATACGATCCCATTTGTCTCCCGCACCTTGTTCATGTCCTTTCCATTCTTCTGCGCCCAAAGCAAATTCATAGTTCCACACACTCTTTTCCGGGGACTGAACATGTCGATGATGCAAATCACAGAAACGATGTAGATGATGGAAGCCATGGACACAAAGCTTGTAGAAGCGAACGCCCGGTGGCATATATTGCTTCCGTAATTCGCAATATCGACACGTTTAATCATTTTATGGGTATAGTAGAACAGTCTAATCTGAGTATATGTGATGTAACTGATGAACTAAAACCCATGAACTTGCTACCTTACATGCACTCTTACAATAGATCAAGTATTAGATTGTTTACTCTGAAATTCAAGTAGCTTTCTTTGTTAAATGCATTAAACTTTGCTCTAAATGATTATTGTAAAAGAAGCAATAGTGGGAATTATAGTTTGAACTTTGCAGATTGATTTAGTTCCATGATTAAAGTTTACTGATAGTTGTAAGTGTTCAATATTCTGCTTTATTGTTTACATATAATTCTAAAGGCTAATCGTAAATTTTTTACTTTGTCTCGAAAAAACTTTAATTTATAAAGGGTAAGTTGGCAAAATAAAGATGAAAGAATGCCTTAATTGAAAAAGAAAAAAAAACCCGCAAACCCAAGCCGAGTGGGTTAAAATATGTTTGAGCCTAGAACGGGTGACTTGACATTTGATAGTCAAGACTATTTTAAATAATTTTGAGCCTAGATCGGGGTTGGATCGAACTTGGGCTAGACTTGGTTAGCTCATGAGTATTAATTATTTAATTAAATTTATAGAATTTATGTTATCTATTTATTTTTTGATATTTTTTTTATATTATAAAATTTTATTTATATATTAAAAAAAACCATTTTATTACTTTTTAAACATTTTTCAAATTTTTTACTAACTTAAAAGCAATGATATATAAATTATAAATTTATTTAAAAGTTTAATTTTAAAATATATAAAATATAATTAAATTGACTTTAATTATAAGTTGATTGGACAAAGTCACTATTATAAAGGTAATCTAATCAGTAAAATTTAAAGATTAGACCAAACTTTGACGGAGTCGGAGGTGAAGTTGGGGTAAAGTTTGGGCCAAAAGCCCATTAATAAGAGTAGACTTTTAATATATTAATTAGACCCTCATCCACGATGCTCGCGTGGAAGGAAAAAACAAAAAAAGCAAATAACGGGATTTCTTGAGAATAAAGTGAGGTGCATACTTTAGAGAAATGATTTGCCAGTGATTTCATTGCTGTTCTTGCATTGCCTTACGTGGTGTTCGTGTAGATTTGCTTGTCTTTTCAGCTTTGATGGGTTTCTCTTTATCTTACCAACTGATTCTTTGAAATGTCGAGGATCAATTGAGTGGAGCACAACTGATCAATTCAGAATAAGACACTGCAGAAATCGCTTGGATTCTTGTACTTTTAAGAGAAAATCAAACAGGTAAAATACCCATTTCGTGTACGCATTTAATCTCTCTTTTTCAAGAAATCGAACTTTTCTCCTGGTTTTGTGATCTTGCATTTCATCCGCGTGAGGGTCTGTTAGCTTAGTTTCAGCGCATTTTGA

mRNA sequence

ATTCTCGCCACTTTCCAGTTGCCATTGCGATTTGATCGCTGCGGCTCGCACTGTCTGTACAATCCGACCACCAACCATGGCGGAGGAGCTCGGCATCGACGTCTCTGTTCCTCCTAGCCTCCATCTCATCTCTGCTTTCCTTGCCATGGAGCCAGCTGATTCTTTACTTTGCATGGCCAGGGAATTGGGTCGCGGATCAGTGACCGAGACAGTGCAGAGGTTCATTTGGGATCATTGCATTAGCAAAGCTCAAGAAATCAACCATTTTCATGTACCATATCTGAAGAATTTTCTGAAGAAGCTGATATCTGAAGTTGAATCAAGTCAAGTTGAAGTGTTAGATGAGTTCTATGAATTGTATGCTCATTACATGGTTTCATGGAAGGATGAGAATCTGGGCAAAGAAAGTGCAACAATCTCCAAATTTGTTTCTTTTCTTTTTCCACCTGGTTCTTCAAGTTGTCAAAAGTTAAGGAAATTTGTGGTGCCACTACAATGCTCTCTCAATATGCTTGCAGGTGATACAGGGAATGCTTCACACTCTCAACTTTGTTTAAGTTATCTTATGTGTTCACTTTGGCCTTCGAGTCTATACCTTTCGGAACTGATACTCTCGTTTCCAAACATTTTCTCAACAAGAAAATGCTTTGAGGTTGGTTCGGGTGTGGGTTTGGTTGGAATCTGCCTTGCTCGTGTCAAAACGTCATCGATAGTGTTGAGTGATGGTGACCTATCAACATTAGCAAATATGAAGGTTAATTTAAGGTTGAATGGGTTGTGCAGCCTCAGCTCTGCAACTGCTACACCAGAAAGAACTAAAGAGGATCTTCAAACTGTGGAATGCATTCATCTGCCGTGGGAATCAGCATCAGAAAGTGAACTCCAAGCTTTTGCACCAGATATTGTGTTAGGCGCAGATGTAATATACGATCCCATTTGTCTCCCGCACCTTGTTCATGTCCTTTCCATTCTTCTGCGCCCAAAGCAAATTCATAGTTCCACACACTCTTTTCCGGGGACTGAACATGTCGATGATGCAAATCACAGAAACGATGTAGATGATGGAAGCCATGGACACAAAGCTTGTAGAAGCGAACGCCCGGTGGCATATATTGCTTCCGTAATTCGCAATATCGACACGTTTAATCATTTTATGGGTATAGTAGAACAGTCTAATCTGAGTATATGTGATGTAACTGATGAACTAAAACCCATGAACTTGCTACCTTACATGCACTCTTACAATAGATCAAGTATTAGATTGTTTACTCTGAAATTCAAATTTGCTTGTCTTTTCAGCTTTGATGGGTTTCTCTTTATCTTACCAACTGATTCTTTGAAATGTCGAGGATCAATTGAGTGGAGCACAACTGATCAATTCAGAATAAGACACTGCAGAAATCGCTTGGATTCTTGTACTTTTAAGAGAAAATCAAACAGTTTCAGCGCATTTTGA

Coding sequence (CDS)

ATGGCGGAGGAGCTCGGCATCGACGTCTCTGTTCCTCCTAGCCTCCATCTCATCTCTGCTTTCCTTGCCATGGAGCCAGCTGATTCTTTACTTTGCATGGCCAGGGAATTGGGTCGCGGATCAGTGACCGAGACAGTGCAGAGGTTCATTTGGGATCATTGCATTAGCAAAGCTCAAGAAATCAACCATTTTCATGTACCATATCTGAAGAATTTTCTGAAGAAGCTGATATCTGAAGTTGAATCAAGTCAAGTTGAAGTGTTAGATGAGTTCTATGAATTGTATGCTCATTACATGGTTTCATGGAAGGATGAGAATCTGGGCAAAGAAAGTGCAACAATCTCCAAATTTGTTTCTTTTCTTTTTCCACCTGGTTCTTCAAGTTGTCAAAAGTTAAGGAAATTTGTGGTGCCACTACAATGCTCTCTCAATATGCTTGCAGGTGATACAGGGAATGCTTCACACTCTCAACTTTGTTTAAGTTATCTTATGTGTTCACTTTGGCCTTCGAGTCTATACCTTTCGGAACTGATACTCTCGTTTCCAAACATTTTCTCAACAAGAAAATGCTTTGAGGTTGGTTCGGGTGTGGGTTTGGTTGGAATCTGCCTTGCTCGTGTCAAAACGTCATCGATAGTGTTGAGTGATGGTGACCTATCAACATTAGCAAATATGAAGGTTAATTTAAGGTTGAATGGGTTGTGCAGCCTCAGCTCTGCAACTGCTACACCAGAAAGAACTAAAGAGGATCTTCAAACTGTGGAATGCATTCATCTGCCGTGGGAATCAGCATCAGAAAGTGAACTCCAAGCTTTTGCACCAGATATTGTGTTAGGCGCAGATGTAATATACGATCCCATTTGTCTCCCGCACCTTGTTCATGTCCTTTCCATTCTTCTGCGCCCAAAGCAAATTCATAGTTCCACACACTCTTTTCCGGGGACTGAACATGTCGATGATGCAAATCACAGAAACGATGTAGATGATGGAAGCCATGGACACAAAGCTTGTAGAAGCGAACGCCCGGTGGCATATATTGCTTCCGTAATTCGCAATATCGACACGTTTAATCATTTTATGGGTATAGTAGAACAGTCTAATCTGAGTATATGTGATGTAACTGATGAACTAAAACCCATGAACTTGCTACCTTACATGCACTCTTACAATAGATCAAGTATTAGATTGTTTACTCTGAAATTCAAATTTGCTTGTCTTTTCAGCTTTGATGGGTTTCTCTTTATCTTACCAACTGATTCTTTGAAATGTCGAGGATCAATTGAGTGGAGCACAACTGATCAATTCAGAATAAGACACTGCAGAAATCGCTTGGATTCTTGTACTTTTAAGAGAAAATCAAACAGTTTCAGCGCATTTTGA

Protein sequence

MAEELGIDVSVPPSLHLISAFLAMEPADSLLCMARELGRGSVTETVQRFIWDHCISKAQEINHFHVPYLKNFLKKLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPGSSSCQKLRKFVVPLQCSLNMLAGDTGNASHSQLCLSYLMCSLWPSSLYLSELILSFPNIFSTRKCFEVGSGVGLVGICLARVKTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATATPERTKEDLQTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVLSILLRPKQIHSSTHSFPGTEHVDDANHRNDVDDGSHGHKACRSERPVAYIASVIRNIDTFNHFMGIVEQSNLSICDVTDELKPMNLLPYMHSYNRSSIRLFTLKFKFACLFSFDGFLFILPTDSLKCRGSIEWSTTDQFRIRHCRNRLDSCTFKRKSNSFSAF

Homology

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BLAST of CmaCh05G011570 vs. ExPASy Swiss-Prot
Match: Q96G04 (Protein-lysine N-methyltransferase EEF2KMT OS=Homo sapiens OX=9606 GN=EEF2KMT PE=1 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 9.1e-17
Identity = 70/234 (29.91%), Postives = 108/234 (46.15%), Query Frame = 0


Query: 66  VPYLKNFLKKLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPG 125
           V Y + FL +LI + E+   E LDE YE  A  +++                        
Sbjct: 67  VKYARCFLSELIKKHEAVHTEPLDELYEALAETLMA------------------------ 126

Query: 126 SSSCQKLRKFVVPLQCSLNMLAGDTGNASHSQLCLSYLMCSL--WPSSLYLSELILSFPN 185
             S Q  R +++P        +G +   S S   +SY    L  W ++LYL+E  +  P 
Sbjct: 127 KESTQGHRSYLLP--------SGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPA 186

Query: 186 IFSTRKCFEVGSGVGLVGICLARV-KTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATA 245
           +F+ R   E+GSG GL G+ + ++ +  + + SD     L  ++ N+ LNGL   +  TA
Sbjct: 187 VFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITA 246

Query: 246 TPERTKEDLQTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVL 297
                K D   V    L W+ A+  +L AF PD+V+ ADV+Y P  +  LV VL
Sbjct: 247 -----KLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVL 263

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BLAST of CmaCh05G011570 vs. ExPASy Swiss-Prot
Match: Q86JB0 (Putative uncharacterized protein DDB_G0277003 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0277003 PE=4 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-14
Identity = 69/244 (28.28%), Postives = 110/244 (45.08%), Query Frame = 0


Query: 168 WPSSLYLSELILSFPNIFSTRKCFEVGSGVGLVGICLARVK-TSSIVLSDGDLSTLANMK 227
           W ++  LS+ ILS  N+F  +   E+GSG GLVGI L  +K    ++L+D     L N+K
Sbjct: 149 WGAAYQLSDFILSNQNLFINKNILELGSGTGLVGIILDFIKPLKKVILTDYSPKVLKNLK 208

Query: 228 VNLRLNGL-----CSLSSATATPERTKEDLQTVECIH-LPWESASESELQAFA----PDI 287
            N+ LN L      +           KE+   +  +  L WE    + L  ++     +I
Sbjct: 209 FNMELNNLEIQDFINDDDDDNNNNVNKENDDKINQVRVLDWEIEDLNILNNYSGLNDSNI 268

Query: 288 VLGADVIYDPICLPHLVHVLSILLRPKQIHSSTHSFPGTEHVDDANHRNDVDDGSHGHKA 347
           +LGAD++Y+P    +LV +L  LL                                    
Sbjct: 269 ILGADIVYEPSLCKYLVSILYFLLE----------------------------------- 328

Query: 348 CRSERPVAYIASVIRNIDTFNHFMGIVEQSNLSICDVTDELKPMN-LLPYMHSYNRSSIR 400
            R+E  VAYI+S IRN  TF+ F   +   NL++ D+T + +  +   P++  Y+RS I 
Sbjct: 329 -RNENSVAYISSTIRNQSTFSIFQKELNLKNLTVIDITKQFEQSSPTSPFI--YDRSQIV 354

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BLAST of CmaCh05G011570 vs. ExPASy Swiss-Prot
Match: Q1JPJ9 (Protein-lysine N-methyltransferase EEF2KMT OS=Bos taurus OX=9913 GN=EEF2KMT PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 7.2e-14
Identity = 68/238 (28.57%), Postives = 105/238 (44.12%), Query Frame = 0


Query: 66  VPYLKNFLKKLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPG 125
           V Y ++FL +LI + E+   E LDE Y+  A  + +    +  +         S+L P G
Sbjct: 67  VKYSRSFLSELIRKHEAVHTEPLDELYQALAEVLTAEDPTHCHR---------SYLLPSG 126

Query: 126 SSSCQKLRKFVVPLQCSLNMLAGDTGNASHSQLCLSYLMCSLWPSSLYLSELILSFPNIF 185
            S                  L   T   SH    L       W ++LYL+E  +  P +F
Sbjct: 127 DSV----------------TLCESTAIVSHGTTGL-----VTWNAALYLAEWAVENPAVF 186

Query: 186 STRKCFEVGSGVGLVGICLARV-KTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATAT- 245
           + R   E+GSG GL G+ + +  +  + + SD     L  ++ N+ LNG  SL  +  T 
Sbjct: 187 AHRMVLELGSGAGLTGLAICKTCRPRAYIFSDCHSHVLEQLRGNVLLNGF-SLEPSIDTW 246

Query: 246 -----PERTKEDLQTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVL 297
                P   + +   V    L W++ +  +L AF PD+VL ADV+Y P  +  LV VL
Sbjct: 247 AQHPGPHTPEAERPWVTVARLDWDTVTAPQLAAFQPDVVLAADVLYCPETVLSLVGVL 273

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BLAST of CmaCh05G011570 vs. ExPASy Swiss-Prot
Match: P0C5J1 (Putative protein N-methyltransferase FAM86B2 OS=Homo sapiens OX=9606 GN=FAM86B2 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.6e-13
Identity = 71/236 (30.08%), Postives = 106/236 (44.92%), Query Frame = 0


Query: 66  VPYLKNFLKKLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPG 125
           V Y   FL +LI + E+   E LD+ YE+ A  +       + KES    +  S+L   G
Sbjct: 67  VKYAWCFLSELIKKHEAVHTEPLDKLYEVLAETL-------MAKESTQGHR--SYLLSSG 126

Query: 126 SSSCQKLRKFVVPLQCSLNMLA-GDTGNASHSQLCLSYLMCSLWPSSLYLSELILSFPNI 185
            S         V L  S  +++ G TG  +             W ++LYL+E  +  P  
Sbjct: 127 GS---------VTLSKSTAIISHGTTGLVT-------------WDAALYLAEWAIENPAA 186

Query: 186 FSTRKCFEVGSGVGLVGICLARV-KTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATAT 245
           F  R   E+GSG GL G+ + ++ +  + + SD     L  ++ N+ LNGL   +  T  
Sbjct: 187 FINRTVLELGSGAGLTGLAICKMCRPRAYIFSDPHSRILEQLRGNVLLNGLSLEADITG- 246

Query: 246 PERTKEDLQTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVLSIL 300
                 D   V    L W+ A   +L AF PD+V+ ADV+Y P  +  LV VL  L
Sbjct: 247 ----NLDSPRVTVAQLDWDVAMVHQLSAFQPDVVIAADVLYCPEAIVSLVGVLQRL 266

[Close]

BLAST of CmaCh05G011570 vs. ExPASy Swiss-Prot
Match: Q3UZW7 (Protein-lysine N-methyltransferase EEF2KMT OS=Mus musculus OX=10090 GN=Eef2kmt PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.0e-12
Identity = 69/235 (29.36%), Postives = 103/235 (43.83%), Query Frame = 0


Query: 66  VPYLKNFLKKLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPG 125
           V Y + FL KLI + E+   E LD  YE  A  +++       +ES    +  S+L P G
Sbjct: 67  VKYARCFLSKLIKKHEAVPTEPLDALYEALAEVLMT-------QESTQCHR--SYLLPSG 126

Query: 126 SSSCQKLRKFVVPLQCSLNMLAGDTGNASHSQLCLSYLMCSLWPSSLYLSELILSFPNIF 185
           +S                  L+  T   SH    L       W ++LYL+E  +  P  F
Sbjct: 127 NSV----------------TLSESTAIVSHGTTGL-----VTWDAALYLAEWAIENPAAF 186

Query: 186 STRKCFEVGSGVGLVGICLARV-KTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATATP 245
           + R   E+GSG GL G+ + +     + + SD     L  ++ N+ LNG  SL   T   
Sbjct: 187 TDRTILELGSGAGLTGLAICKACCPRAYIFSDCHAQVLEQLRGNVLLNGF-SLEPHTPID 246

Query: 246 ERTKEDLQTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVLSIL 300
             + +    V    L W+  + S+L AF  D+V+ ADV+Y       LV VL +L
Sbjct: 247 AGSSK----VTVAQLDWDEVTASQLSAFQADVVIAADVLYCWEMTLSLVRVLKML 266

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BLAST of CmaCh05G011570 vs. TAIR 10
Match: AT5G27400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.0e-97
Identity = 200/391 (51.15%), Postives = 251/391 (64.19%), Query Frame = 0


Query: 15  LHLISAFLAMEPADSLLCMARELGRGSVTETVQRFIWDHCISKAQEINHFHVPYLKNFLK 74
           L+++SAFLA+EP DS++ +AR    GS+TE  QRF W+ C+  A   N     Y K  LK
Sbjct: 12  LNVLSAFLAIEPVDSVITLARGCTGGSITENAQRFFWEICVEAAANGNG---SYAKKLLK 71

Query: 75  KLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPGS---SSCQK 134
           KLI+EVE    EVLDE YE YA YM++ K++ L KE+  I+KF+SFLFP GS    SC +
Sbjct: 72  KLINEVELENGEVLDEVYEEYALYMLTSKEDILVKENIRITKFISFLFPEGSYKHPSCPR 131

Query: 135 LRKFVVPLQCSLNMLAGDTGNASHSQLCLSYLMCSLWPSSLYLSELILSFPNIFSTRKCF 194
            RK V+PL CSLNML GDTG             CS+WPSSL+LSE +LSFP +F+ + CF
Sbjct: 132 SRKLVIPLHCSLNMLEGDTG-------------CSIWPSSLFLSEFVLSFPELFANKACF 191

Query: 195 EVGSGVGLVGICLARVKTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATATPERTKEDL 254
           EVGSGVG+VGICLA VK   ++L+DGDL TL+NMK+NL  N L         P   +   
Sbjct: 192 EVGSGVGMVGICLAHVKAKEVILTDGDLLTLSNMKLNLERNHLNYDDEFLKQPGEAQS-- 251

Query: 255 QTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVLSILLR--PKQIHSST 314
             V+C HLPWE+ASESEL  + PDIVLGADVIYDP CLPHL+ VL  LL+  PK+ + S 
Sbjct: 252 TRVKCTHLPWETASESELSQYRPDIVLGADVIYDPSCLPHLLRVLVALLKNPPKRGNGSL 311

Query: 315 HSFPGTEHVDDANHRNDVDDGSHGHKACRSERPVAYIASVIRNIDTFNHFMGIVEQSNLS 374
            +              D D     H        VAYIASVIRN+DTFN  + + +Q +LS
Sbjct: 312 ET-------------EDRDTTQEEHSPA-----VAYIASVIRNVDTFNVCLTLADQMDLS 366

Query: 375 ICDVTDELKP-MNLLPYMHSYNRSSIRLFTL 400
           I DVT EL P   LLPYMHSY+RS++RLF++
Sbjct: 372 ITDVTAELTPRFELLPYMHSYDRSTVRLFSI 366

[Close]

BLAST of CmaCh05G011570 vs. TAIR 10
Match: AT5G27410.2 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.6e-93
Identity = 197/394 (50.00%), Postives = 246/394 (62.44%), Query Frame = 0


Query: 15  LHLISAFLAMEPADSLLCMARELGRGSVTETVQRFIWDHCISKAQEINHFHVPYLKNFLK 74
           L+++SAFLA+EP DS++ +AR    GS+TE  QRF W+ C+  A   N     Y K  LK
Sbjct: 12  LNVLSAFLAIEPVDSVITLARGCTGGSITENAQRFFWEICVEAAANGNG---SYAKKLLK 71

Query: 75  KLISEVESSQVEVLDEFYELYAHYMVSWKDENLGKESATISKFVSFLFPPGS---SSCQK 134
           KLI+EVE    EVLDE YE YA YM++ K++ L KE+  I+KF+SFLFP GS    SC +
Sbjct: 72  KLINEVELENGEVLDEVYEEYALYMLTSKEDILVKENIRITKFISFLFPEGSYKHPSCPR 131

Query: 135 LRKFVVPLQCSLNMLAGDTGNASHSQLCLSYLMCSLWPSSLYLSELILSFPNIFSTRKCF 194
            RK V+PL CSLNML GDTG             CS+WPSSL+LSE +LSFP +F+ + CF
Sbjct: 132 SRKLVIPLHCSLNMLEGDTG-------------CSIWPSSLFLSEFVLSFPELFANKACF 191

Query: 195 EVGSGVGLVGICLARVKTSSIVLSDGDLSTLANMKVNLRLNGLCSLSSATATPERTKEDL 254
           EVGSGVG+VGICLA VK   ++L+DGDL TL+NMK+NL  N L         P   +   
Sbjct: 192 EVGSGVGMVGICLAHVKAKEVILTDGDLLTLSNMKLNLERNHLNYDDEFLKQPGEAQS-- 251

Query: 255 QTVECIHLPWESASESELQAFAPDIVLGADVIYDPICLPHLVHVLSILLR--PKQIHSST 314
             V+C HLPWE+ASESEL  + PDIVLGADVIYDP CLPHL+ VL  LL+  PK+ + S 
Sbjct: 252 TRVKCTHLPWETASESELSQYRPDIVLGADVIYDPSCLPHLLRVLVALLKNPPKRGNGSL 311

Query: 315 HSFPGTEHVDDANHRNDVDDGSHGHKACRSERPVAYIASVIRNIDTFNHFMGIVEQSNLS 374
            +              D D     H        VAYIASVIRN+DTFN  + + +Q +LS
Sbjct: 312 ET-------------EDRDTTQEEHSPA-----VAYIASVIRNVDTFNVCLTLADQMDLS 369

Query: 375 ICDVTDELKP-MNLLPYMHSYNRSSIRLFTLKFK 403
           I DVT EL P   LLPYMHS  R+   +  LK +
Sbjct: 372 ITDVTAELTPRFELLPYMHSETRTKAIIVLLKLR 369

[Close]

BLAST of CmaCh05G011570 vs. TAIR 10
Match: AT4G35987.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 38/143 (26.57%), Postives = 65/143 (45.45%), Query Frame = 0


Query: 163 LMCSLWPSSLYLSELILSFPNIFSTRKCFEVGSGVGLVGICL-ARVKTSSIVLSDGDLST 222
           L+C  WPS   L+    S P  F  ++  E+GSG GL G+ + A  + S +V+SDG+   
Sbjct: 105 LVCQ-WPSEEVLAYFCKSQPERFRGKRVIELGSGYGLAGLVIAAATEASEVVISDGNPQV 164

Query: 223 LANMKVNLRLNGLCSLSSATATPERTKEDLQTVECIHLPWESASESELQAFAPDIVLGAD 282
           +  +K N+  N +    +             +V+ + L W     SEL     DI++ +D
Sbjct: 165 VNYIKRNIETNSMAFGGT-------------SVKAMELHWNQHQLSEL-TNTFDIIVASD 224

Query: 283 VIYDPICLPHLVHVLSILLRPKQ 305
             +       L   + +LL+ K+
Sbjct: 225 CTFFKEFHKDLARTIKMLLKAKK 232

[Close]

BLAST of CmaCh05G011570 vs. TAIR 10
Match: AT1G79915.1 (Putative methyltransferase family protein )

HSP 1 Score: 43.5 bits (101), Expect = 5.3e-04
Identity = 51/162 (31.48%), Postives = 72/162 (44.44%), Query Frame = 0


Query: 81  ESSQVEVLDEFYELYAHYMVSWKDENLG-KESATISKFVS---FLFPPGSSSCQKLRKFV 140
           E S++EV+ E +              LG   +AT   F+S   F FP  + S  K    +
Sbjct: 3   EDSEIEVMSEIH--------------LGCPPAATDDPFLSRFTFSFPSNTVSVDKDGDLL 62

Query: 141 VPLQCSLNMLAGD--TGNASHSQLC-LSYLMCSLWPSSLYLSELIL---SFPNIFSTRKC 200
           +P +   N   G   T    HS    L ++   +W + L LSE +L      + F    C
Sbjct: 63  LPRR---NYPTGPSFTVTIQHSITSNLRHVGLQVWKAQLVLSEFVLHKICTSSDFHGIVC 122

Query: 201 FEVGSGVGLVGICLARVKTSSIVLSDGDLSTLANMKVNLRLN 233
            E+G+G GL+GI LARV   +I L+D     L N   NL LN
Sbjct: 123 LELGAGTGLLGILLARV-AKAIFLTDHGDEILGNCGKNLELN 146

The following BLAST results are available for this feature:

BLAST of CmaCh05G011570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q96G04	9.1e-17	29.91	Protein-lysine N-methyltransferase EEF2KMT OS=Homo sapiens OX=9606 GN=EEF2KMT PE...	[more]
Q86JB0	1.9e-14	28.28	Putative uncharacterized protein DDB_G0277003 OS=Dictyostelium discoideum OX=446...	[more]
Q1JPJ9	7.2e-14	28.57	Protein-lysine N-methyltransferase EEF2KMT OS=Bos taurus OX=9913 GN=EEF2KMT PE=2...	[more]
P0C5J1	1.6e-13	30.08	Putative protein N-methyltransferase FAM86B2 OS=Homo sapiens OX=9606 GN=FAM86B2 ...	[more]
Q3UZW7	3.0e-12	29.36	Protein-lysine N-methyltransferase EEF2KMT OS=Mus musculus OX=10090 GN=Eef2kmt P...	[more]

BLAST of CmaCh05G011570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27400.1	7.0e-97	51.15	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G27410.2	1.6e-93	50.00	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT4G35987.1	1.4e-04	26.57	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G79915.1	5.3e-04	31.48	Putative methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029426	FAM86, N-terminal	PFAM	PF14904	FAM86	coord: 65..96 e-value: 1.4E-5 score: 25.1
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 142..389 e-value: 3.6E-34 score: 120.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 316..335
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 312..335
None	No IPR available	PANTHER	PTHR14614:SF130	S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN	coord: 25..400
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 167..303 e-value: 2.3E-12 score: 47.0
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 25..400
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 163..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh05G011570.1	CmaCh05G011570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008483	transaminase activity