CmaCh05G010180 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh05G010180
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	GDP-L-fucose synthase
Location	Cma_Chr05: 8177442 .. 8181690 (+)
RNA-Seq Expression	CmaCh05G010180
Synteny	CmaCh05G010180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATACGCCCTCACATTTTCTTCCTCCTGTCCCCGGCCGCCGACGATTCTCTCCTCCACGTCCGACGATGGGCGGCGCCGCCGACAACGGTACTCTCTCTATTCACTCGATGTTTATTCGTGTTTCTTAGACACTACTGTAAATCCTTTCTGAAAATTTTCTTTTCTCTTTGAATTTTGCAGCTTCATCAGGATCCTCGTTCCTCTCTGATAAATCGGCGAAGATCTTCATCGCCGGTCACCGTGGCCTCGTCGGTTCCGCCATTGTCCGCAAGCTCCAGCAGCTTGGATTCACGAATCTCCTTCTTCGTTCTCACTCCGAGCTTGACCTAACTCGCCAATCCGATGTCGAATCGTTCTTCGCTAATGAAAAACCTCGATTCGCTATCCTCGCTGCCGCTAAAGTCGGCGGCATTCACGCCAACAACACCTATCCGGCGGATTTCATCGCCATTAACCTTCAAATCCAGACTAATGTCATCGATTCGGCCTATCGCCATGGTGTTCAGAAGCTATTGTTCCTCGGTTCGTCGTGTATTTATCCGAAATTCGCTCCACAGCCGATTCCCGAAGACGCATTGCTGACCGGTCCTCTGGAGCCGACGAACGAGTGGTACGCGGTGGCGAAGATCGCCGGAATCAAAATGTGTCAGGCGTATCGAATTCAACACAATTGGAATGCGATTTCAGGAATGCCGACAAATCTGTACGGTCCCAACGACAATTTCCACCCGGAGAATTCACACGTCTTGCCGGCATTGATGAGGAGATTTCACGAGGCAAAGGTGAACGGAGCAAAAGAAGTAGTGGTTTGGGGAAGTGGAAGCCCTCTCAGGGAGTTCCTGCACGTCGATGATTTGGCCGACGCCGTCGCTTTCCTCATGGAGGAATACAGCGATTTAGGCCATGTGAATGTTGGGAGTGGGAAGGAAGTGAGCATCAAAGAGCTAGCTGAATTGGTTAAAGAAGTGGTGGGATTTGAAGGAGATCTTGTTTGGGACAAATCAAAGCCTGATGGAACTCCAAGGAAATTGATGGACAGTTCCAAGCTTGCTGAATTGGGATGGAATCCAAGGATTTCTCTGAGGGATGGTCTTGTGGATACTTACAAATGGTATGTTCAAAATGTGCAGCAATGAAAAACGAACAAGAACACAGGTGAGTCCACCATTTTCAATGATCTGTTTGATCCTTTTGTTGTGATTTTAGTTTATAGATCTGTATTACTGAATCAAATTTGCTTAGATCTGAAGCTTTTGCTGCTGCCTGATATATGATTTGATGCCTATAGAGATTGCTGTCATATCTTTCTTTGCCAATTATGTTGATCTTCTTACCAGTAGATTGAGCCAGTTTGTATTTCTACAATTGAGAATAAAATTTGCTGATTTGAGAACCATTTCTTTCTTACAATTTGGTTTGCTGCAAGGCTTAGGGAATTTGAAATGTTTGTGGGTTTGAAGATCTGTGGTGAGTAAAATTCATTTCTTACTTTGTGCAAGTCTGAGATACCTCCCATGGCAAATTCATAAAAAGACAATTTCCTCTTCCCTTTACCCGTGGGTGAATTAGAGGAAAACAAGTTCTCATCTGAGGAGAAATAATTTGTGGACCTAACATGTTCTGATATCTCCCGACCGGTAGGAAAGAAACCTCTAACCAATGACTAGTTTTAGTCTAGAGTTACAAGTTCTATTTAGCACATTAAGACTACCTTTTTTGAAAGAGTGATTATCTATGGCTTGAAGGAAACCTGTAACCAATGACTAGTTTCATTGTCGAGTTGCGAGTTCTATTTAGCACATTAAGACTTCCTTTTGAAAAAGTGATTATCTATGGCTTGAGGGAAACCTATAACCAATGACTAGTTTCACTGTCGGGTTGCGAGTTCTATTTAGCACATTAAGACTACCTTTTGAAAAAGTGATTACCTATGGCTTGAAGGAAGGAAACCTGTAACCAATGACTAACTCATTGTCGAGTTGCGAGTTCTATTTAGCACATTAAGACTTCCTTTTGAAAGAGTGATTATCTATAGCTTGAGGGAAACCTATAACCAATGACTAGTTTCACTGTCGGGTTGCGAGTTCTATTTAGCACATTAAGACTACCTTTTGAAAAAGTGATTACCTATGGCTCGAAGGAAGGAAACCTGTAACCAATGACTAGTTTCATTGTTGAGTTACAAGTTCTATTTAGCACAATAAGACTTCCTTTTTGAAAGATTTGATTATCTATGGCTTGAGGTAAGCAACCTGTAACCAGTGACTAGTTTCATTGTCGAGTTGTGAGTTCTATTTAGTACATTAAGACTACCTTTTGAAAAAGTGATTACCAATGGCTTGAAGGAAACCTGTAACCAATGACTAGTTTCATTGTCGAGTTGTGAGTTCTATTTAGCACATTAAGACTACCTATTGAAAGAGTGATTCCTATGGCTTGAAGGATGGAGAAAGATCGCTCCCGTAGCCAATAACTAGTTCCTCTATAGAGCTACGTGGAGAATACTCCTTTTCAAAAGTGAGATCGCTAATAGCTTGAAGAAGCTAGAAAAACCACTCCTAAATGCTCTGAAAAAGGTAGAAGGTAGCCTTGAGATTGTTGGTTTATGTTACTAAAAGCAAACATTATGAAATTAGAGAGAAATGCGAGAATTAAATAGATACTTACTGAGCGTTCATTAAATTTGAGCAAATTTTCATATATTCGTGGAAAGAATTATGTGATATTATTTTTACTATTTCAATGGAAATTTTAAATAAAATTGTTGGATGAAAAAGAAAAGTCTCACATCGGCTAATATAGGAAATAATCATGAGTTTATAATCTAGGAATATTATGTTATGAGGCCTTTTGTGGTAGTCCAAAGTAAAGCAATGAGAGTTTATGCTCAAAGTAGACAATATCATACCATTGTGGAGAGTTGTGTTCATCCAACATGGTATCAGAGTCGTGCTGTAAATTTAGTCATGTCAATAGAATCCTCAAATGACGAACAAAGAGCTCCAAAAGGAGACTACTCCTCGAAGGCATAGTAAAAAATGACTCAGACTCCAAAAGAAAAGGAGTCGAGCCTCGATTAAGTGGAGGTGTACTTTGTTCGAGGGGAGGTGTTGGATGAAAAAGAAAAGTCCCACATCGGCTAATTAAGTGGACAATATCATACCAATGTGGAGAGTCTGGTTCATCTAACAAAAACAATTAAAAATAGCTACAAATCACGAACAAAATATCTAAAATAATTCATTTACAAAAAATTTAGGGTTTTGAGTATAAATAGATATTTACAAAAGAAAGGAAAAATATAAAGGAAGGGTCAACACAAATTTGGGAGTAAAAAAATAGAATGATATTTTAGAGAAAAGTGAGAAGAAAAATTCCAAAATAGATTCACATAAAATAACAAAACCCTAAGACCCATCATTTTCACTAAAATCTTATGTTCCCATGTAATCTCCATCCCCTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCCATATTTACCACATCCACCAAACCAAACCCTAAACCTTATTTAATACTCGAACCTTCCATGAATTACTTATTTTTAAACAAGGATATAACGAACTCACCTTGTCTCCACGCAGGATATCGAGAACGGTATAAGATGGATCAAAAATTTCTAATTTGTATAGGGTCGTTGAACTGGCCCAACCAACTAAAACTATATGCATTATATGAATAGAAAGTAAACGACCTTGATCATTCAATACTACCGTCCAAGACAAACCTTGGAAATACCCATTTGATGGCAGCAGACTTGCGATTGTGCATCACGTACTTTCCAGCAACAAGTGAGTTAAGTCTATTCGTTATTGTGTCGCTTACAGCTAAATGACGTATTCTCGAGCCTCTAGC

mRNA sequence

ATACGCCCTCACATTTTCTTCCTCCTGTCCCCGGCCGCCGACGATTCTCTCCTCCACGTCCGACGATGGGCGGCGCCGCCGACAACGCTTCATCAGGATCCTCGTTCCTCTCTGATAAATCGGCGAAGATCTTCATCGCCGGTCACCGTGGCCTCGTCGGTTCCGCCATTGTCCGCAAGCTCCAGCAGCTTGGATTCACGAATCTCCTTCTTCGTTCTCACTCCGAGCTTGACCTAACTCGCCAATCCGATGTCGAATCGTTCTTCGCTAATGAAAAACCTCGATTCGCTATCCTCGCTGCCGCTAAAGTCGGCGGCATTCACGCCAACAACACCTATCCGGCGGATTTCATCGCCATTAACCTTCAAATCCAGACTAATGTCATCGATTCGGCCTATCGCCATGGTGTTCAGAAGCTATTGTTCCTCGGTTCGTCGTGTATTTATCCGAAATTCGCTCCACAGCCGATTCCCGAAGACGCATTGCTGACCGGTCCTCTGGAGCCGACGAACGAGTGGTACGCGGTGGCGAAGATCGCCGGAATCAAAATGTGTCAGGCGTATCGAATTCAACACAATTGGAATGCGATTTCAGGAATGCCGACAAATCTGTACGGTCCCAACGACAATTTCCACCCGGAGAATTCACACGTCTTGCCGGCATTGATGAGGAGATTTCACGAGGCAAAGGTGAACGGAGCAAAAGAAGTAGTGGTTTGGGGAAGTGGAAGCCCTCTCAGGGAGTTCCTGCACGTCGATGATTTGGCCGACGCCGTCGCTTTCCTCATGGAGGAATACAGCGATTTAGGCCATGTGAATGTTGGGAGTGGGAAGGAAGTGAGCATCAAAGAGCTAGCTGAATTGGTTAAAGAAGTGGTGGGATTTGAAGGAGATCTTGTTTGGGACAAATCAAAGCCTGATGGAACTCCAAGGAAATTGATGGACAGTTCCAAGCTTGCTGAATTGGGATGGAATCCAAGGATTTCTCTGAGGGATGGTCTTGTGGATACTTACAAATGGTATGTTCAAAATGTGCAGCAATGAAAAACGAACAAGAACACAGGATATCGAGAACGGTATAAGATGGATCAAAAATTTCTAATTTGTATAGGGTCGTTGAACTGGCCCAACCAACTAAAACTATATGCATTATATGAATAGAAAGTAAACGACCTTGATCATTCAATACTACCGTCCAAGACAAACCTTGGAAATACCCATTTGATGGCAGCAGACTTGCGATTGTGCATCACGTACTTTCCAGCAACAAGTGAGTTAAGTCTATTCGTTATTGTGTCGCTTACAGCTAAATGACGTATTCTCGAGCCTCTAGC

Coding sequence (CDS)

ATGGGCGGCGCCGCCGACAACGCTTCATCAGGATCCTCGTTCCTCTCTGATAAATCGGCGAAGATCTTCATCGCCGGTCACCGTGGCCTCGTCGGTTCCGCCATTGTCCGCAAGCTCCAGCAGCTTGGATTCACGAATCTCCTTCTTCGTTCTCACTCCGAGCTTGACCTAACTCGCCAATCCGATGTCGAATCGTTCTTCGCTAATGAAAAACCTCGATTCGCTATCCTCGCTGCCGCTAAAGTCGGCGGCATTCACGCCAACAACACCTATCCGGCGGATTTCATCGCCATTAACCTTCAAATCCAGACTAATGTCATCGATTCGGCCTATCGCCATGGTGTTCAGAAGCTATTGTTCCTCGGTTCGTCGTGTATTTATCCGAAATTCGCTCCACAGCCGATTCCCGAAGACGCATTGCTGACCGGTCCTCTGGAGCCGACGAACGAGTGGTACGCGGTGGCGAAGATCGCCGGAATCAAAATGTGTCAGGCGTATCGAATTCAACACAATTGGAATGCGATTTCAGGAATGCCGACAAATCTGTACGGTCCCAACGACAATTTCCACCCGGAGAATTCACACGTCTTGCCGGCATTGATGAGGAGATTTCACGAGGCAAAGGTGAACGGAGCAAAAGAAGTAGTGGTTTGGGGAAGTGGAAGCCCTCTCAGGGAGTTCCTGCACGTCGATGATTTGGCCGACGCCGTCGCTTTCCTCATGGAGGAATACAGCGATTTAGGCCATGTGAATGTTGGGAGTGGGAAGGAAGTGAGCATCAAAGAGCTAGCTGAATTGGTTAAAGAAGTGGTGGGATTTGAAGGAGATCTTGTTTGGGACAAATCAAAGCCTGATGGAACTCCAAGGAAATTGATGGACAGTTCCAAGCTTGCTGAATTGGGATGGAATCCAAGGATTTCTCTGAGGGATGGTCTTGTGGATACTTACAAATGGTATGTTCAAAATGTGCAGCAATGA

Protein sequence

MGGAADNASSGSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVESFFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYGPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEYSDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNPRISLRDGLVDTYKWYVQNVQQ

Homology

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BLAST of CmaCh05G010180 vs. ExPASy Swiss-Prot
Match: Q9LMU0 (Putative GDP-L-fucose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=GER2 PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 4.6e-156
Identity = 267/324 (82.41%), Postives = 288/324 (88.89%), Query Frame = 0


Query: 5   ADNASS---GSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQS 64
           ADN  S     SF+ +KSAKIF+AGHRGLVGSAIVRKLQ  GFTNL+LR+HSELDLT QS
Sbjct: 2   ADNTGSEMKSGSFMLEKSAKIFVAGHRGLVGSAIVRKLQDQGFTNLVLRTHSELDLTSQS 61

Query: 65  DVESFFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFL 124
           DVESFFA EKP + ILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY HGV+KLLFL
Sbjct: 62  DVESFFATEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYTHGVKKLLFL 121

Query: 125 GSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTN 184
           GSSCIYPKFAPQPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYR+QH W+AISGMPTN
Sbjct: 122 GSSCIYPKFAPQPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRLQHQWDAISGMPTN 181

Query: 185 LYGPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLM 244
           LYG NDNFHPENSHVLPALMRRFHEAK N A EVVVWGSGSPLREFLHVDDLADA  FLM
Sbjct: 182 LYGQNDNFHPENSHVLPALMRRFHEAKANNADEVVVWGSGSPLREFLHVDDLADACVFLM 241

Query: 245 EEYSDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELG 304
           ++YS   HVNVGSG EV+IKELAELVKEVVGF+G LVWD +KPDGTPRKLMDSSKLA LG
Sbjct: 242 DQYSGFEHVNVGSGVEVTIKELAELVKEVVGFKGKLVWDTTKPDGTPRKLMDSSKLASLG 301

Query: 305 WNPRISLRDGLVDTYKWYVQNVQQ 326
           W P+ISL+DGL  TY+WY++NV Q
Sbjct: 302 WTPKISLKDGLSQTYEWYLENVVQ 325

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BLAST of CmaCh05G010180 vs. ExPASy Swiss-Prot
Match: O49213 (GDP-L-fucose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=GER1 PE=1 SV=3)

HSP 1 Score: 547.0 bits (1408), Expect = 1.5e-154
Identity = 260/319 (81.50%), Postives = 288/319 (90.28%), Query Frame = 0


Query: 5   ADNASSGSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVE 64
           A+   S  S +SDKSAKIF+AGHRGLVGSAIVRKLQ+ GFTNL+L++H+ELDLTRQ+DVE
Sbjct: 2   AETIGSEVSSMSDKSAKIFVAGHRGLVGSAIVRKLQEQGFTNLVLKTHAELDLTRQADVE 61

Query: 65  SFFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSS 124
           SFF+ EKP + ILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY HGV+KLLFLGSS
Sbjct: 62  SFFSQEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYEHGVKKLLFLGSS 121

Query: 125 CIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYG 184
           CIYPKFAPQPIPE ALLT  LEPTNEWYA+AKIAGIK CQAYRIQH W+AISGMPTNLYG
Sbjct: 122 CIYPKFAPQPIPESALLTASLEPTNEWYAIAKIAGIKTCQAYRIQHGWDAISGMPTNLYG 181

Query: 185 PNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEY 244
           PNDNFHPENSHVLPALMRRFHEAKVNGA+EVVVWG+GSPLREFLHVDDLADA  FL++ Y
Sbjct: 182 PNDNFHPENSHVLPALMRRFHEAKVNGAEEVVVWGTGSPLREFLHVDDLADACVFLLDRY 241

Query: 245 SDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNP 304
           S L HVN+GSG+EV+I+ELAELVKEVVGFEG L WD +KPDGTPRKLMDSSKLA LGW P
Sbjct: 242 SGLEHVNIGSGQEVTIRELAELVKEVVGFEGKLGWDCTKPDGTPRKLMDSSKLASLGWTP 301

Query: 305 RISLRDGLVDTYKWYVQNV 324
           ++SLRDGL  TY WY++NV
Sbjct: 302 KVSLRDGLSQTYDWYLKNV 320

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BLAST of CmaCh05G010180 vs. ExPASy Swiss-Prot
Match: Q67WR2 (Probable GDP-L-fucose synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0652400 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 6.0e-148
Identity = 249/322 (77.33%), Postives = 287/322 (89.13%), Query Frame = 0


Query: 3   GAADNASSGSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSD 62
           G    A   +SFL+DK  K+F+AGHRGLVGSAI+R L  LGFTN+++R+H+ELDLTRQSD
Sbjct: 2   GTVTTADPHASFLADKGGKVFVAGHRGLVGSAILRHLVSLGFTNVVVRTHAELDLTRQSD 61

Query: 63  VESFFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHG-VQKLLFL 122
           VE+FFA E PR+ +LAAAKVGGIHAN+T+PADFIA NLQIQTNV+D+A + G V+KLLFL
Sbjct: 62  VEAFFAAELPRYVVLAAAKVGGIHANSTFPADFIAANLQIQTNVVDAALKCGSVRKLLFL 121

Query: 123 GSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTN 182
           GSSCIYPKFAPQPIPE++LL+GPLEPTNEWYAVAKIAGIKMCQAYRIQH ++AIS MPTN
Sbjct: 122 GSSCIYPKFAPQPIPENSLLSGPLEPTNEWYAVAKIAGIKMCQAYRIQHGFDAISAMPTN 181

Query: 183 LYGPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLM 242
           LYGP DNFHPENSHVLPAL+RRFHEAK + A EVVVWG+GSPLREFLHVDDLADAV FLM
Sbjct: 182 LYGPQDNFHPENSHVLPALIRRFHEAKASNAAEVVVWGTGSPLREFLHVDDLADAVIFLM 241

Query: 243 EEYSDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELG 302
           + YS L HVNVGSG EV+IKELAELVKEVVGF+G LVWD SKPDGTPRKLMDSSK+ E+G
Sbjct: 242 DHYSGLEHVNVGSGSEVTIKELAELVKEVVGFQGKLVWDSSKPDGTPRKLMDSSKIQEMG 301

Query: 303 WNPRISLRDGLVDTYKWYVQNV 324
           W P++ L++GLV+TYKWYV+NV
Sbjct: 302 WKPKVPLKEGLVETYKWYVENV 323

[Close]

BLAST of CmaCh05G010180 vs. ExPASy Swiss-Prot
Match: Q67WR5 (Putative GDP-L-fucose synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0652300 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 6.0e-108
Identity = 187/318 (58.81%), Postives = 246/318 (77.36%), Query Frame = 0


Query: 6   DNASSGSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVES 65
           D  ++  SFL DKSAK+FIAGHRG+VGSA+ RKL  LGFTN+++R+ +ELDL  Q+ VE+
Sbjct: 21  DGDAAAVSFLGDKSAKVFIAGHRGMVGSAVHRKLDALGFTNVVVRTRAELDLACQAAVEA 80

Query: 66  FFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHG-VQKLLFLGSS 125
           FFA E PR+ ILAAAKVGG+HA++  PA+++  NL+I  NV+D+A R G V+KLL L SS
Sbjct: 81  FFAAELPRYVILAAAKVGGVHASSAAPAEYLTENLRITVNVVDAARRCGSVRKLLVLASS 140

Query: 126 CIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYG 185
            IYP  APQP PE ALLTGP    +EWYA+ KIAGIKMCQA R ++  +AI+  P NLYG
Sbjct: 141 TIYPADAPQPTPESALLTGPPAEGSEWYAIPKIAGIKMCQAVRAEYGLDAIAAAPNNLYG 200

Query: 186 PNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEY 245
           P   F PE+SHV+PAL+RRFH AK+ GA EV VWGSG+  REF HVDDLA+AV  LME Y
Sbjct: 201 PRHPFPPEHSHVIPALIRRFHRAKLEGAGEVAVWGSGAAAREFTHVDDLAEAVVVLMERY 260

Query: 246 SDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNP 305
           S   HVNVGSG+EV+++ELAE V+ VVG+EG + WD ++P+G  R+++DS ++ +LGW P
Sbjct: 261 SGEEHVNVGSGEEVTVRELAEAVRGVVGYEGVVAWDAARPEGVARRVVDSGRMRKLGWEP 320

Query: 306 RISLRDGLVDTYKWYVQN 323
           R++LRDG+ D Y++Y+++
Sbjct: 321 RVALRDGIQDLYRFYLRH 338

[Close]

BLAST of CmaCh05G010180 vs. ExPASy Swiss-Prot
Match: P55353 (GDP-L-fucose synthase OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=fcl PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.0e-102
Identity = 177/303 (58.42%), Postives = 231/303 (76.24%), Query Frame = 0


Query: 21  KIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVESFFANEKPRFAILAAA 80
           +I++AGH+G+VGSAI+R L       +++    +LDLTRQ +VE F   EKP   I+AAA
Sbjct: 10  RIWVAGHKGMVGSAIIRSLASED-CEVIVADRQKLDLTRQEEVEKFLLKEKPHAVIMAAA 69

Query: 81  KVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSSCIYPKFAPQPIPEDAL 140
           KVGGI AN+T PADFI  NL ++ NVI+ ++R GV+KLLFLGSSCIYPK+A QPI E+AL
Sbjct: 70  KVGGILANDTMPADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAAQPIREEAL 129

Query: 141 LTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYGPNDNFHPENSHVLPAL 200
           LTGPLEPTNEWYA+AKIAGIK+CQAYR Q+  N IS MPTNLYGP D F   +SHV+PAL
Sbjct: 130 LTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGANFISAMPTNLYGPRDKFDLNSSHVVPAL 189

Query: 201 MRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEYSDLGHVNVGSGKEVSI 260
           +R+ HEAK+     + +WGSG+P R+FL+ +D +DA+ FL++ YS+  H+N+GSG E+SI
Sbjct: 190 IRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINIGSGGEISI 249

Query: 261 KELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNPRISLRDGLVDTYKWYV 320
            ELA +V  VVGF+GD+V+D SKPDGTPRKL+ S +L  +GW P+ SL  GL  +Y+ +V
Sbjct: 250 IELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGLAKSYESFV 309

Query: 321 QNV 324
            NV
Sbjct: 310 SNV 311

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BLAST of CmaCh05G010180 vs. TAIR 10
Match: AT1G17890.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 552.0 bits (1421), Expect = 3.2e-157
Identity = 267/324 (82.41%), Postives = 288/324 (88.89%), Query Frame = 0


Query: 5   ADNASS---GSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQS 64
           ADN  S     SF+ +KSAKIF+AGHRGLVGSAIVRKLQ  GFTNL+LR+HSELDLT QS
Sbjct: 2   ADNTGSEMKSGSFMLEKSAKIFVAGHRGLVGSAIVRKLQDQGFTNLVLRTHSELDLTSQS 61

Query: 65  DVESFFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFL 124
           DVESFFA EKP + ILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY HGV+KLLFL
Sbjct: 62  DVESFFATEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYTHGVKKLLFL 121

Query: 125 GSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTN 184
           GSSCIYPKFAPQPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYR+QH W+AISGMPTN
Sbjct: 122 GSSCIYPKFAPQPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRLQHQWDAISGMPTN 181

Query: 185 LYGPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLM 244
           LYG NDNFHPENSHVLPALMRRFHEAK N A EVVVWGSGSPLREFLHVDDLADA  FLM
Sbjct: 182 LYGQNDNFHPENSHVLPALMRRFHEAKANNADEVVVWGSGSPLREFLHVDDLADACVFLM 241

Query: 245 EEYSDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELG 304
           ++YS   HVNVGSG EV+IKELAELVKEVVGF+G LVWD +KPDGTPRKLMDSSKLA LG
Sbjct: 242 DQYSGFEHVNVGSGVEVTIKELAELVKEVVGFKGKLVWDTTKPDGTPRKLMDSSKLASLG 301

Query: 305 WNPRISLRDGLVDTYKWYVQNVQQ 326
           W P+ISL+DGL  TY+WY++NV Q
Sbjct: 302 WTPKISLKDGLSQTYEWYLENVVQ 325

[Close]

BLAST of CmaCh05G010180 vs. TAIR 10
Match: AT1G17890.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 5.5e-157
Identity = 263/313 (84.03%), Postives = 284/313 (90.73%), Query Frame = 0


Query: 13  SFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVESFFANEKP 72
           SF+ +KSAKIF+AGHRGLVGSAIVRKLQ  GFTNL+LR+HSELDLT QSDVESFFA EKP
Sbjct: 5   SFMLEKSAKIFVAGHRGLVGSAIVRKLQDQGFTNLVLRTHSELDLTSQSDVESFFATEKP 64

Query: 73  RFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSSCIYPKFAP 132
            + ILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY HGV+KLLFLGSSCIYPKFAP
Sbjct: 65  VYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYTHGVKKLLFLGSSCIYPKFAP 124

Query: 133 QPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYGPNDNFHPE 192
           QPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYR+QH W+AISGMPTNLYG NDNFHPE
Sbjct: 125 QPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRLQHQWDAISGMPTNLYGQNDNFHPE 184

Query: 193 NSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEYSDLGHVNV 252
           NSHVLPALMRRFHEAK N A EVVVWGSGSPLREFLHVDDLADA  FLM++YS   HVNV
Sbjct: 185 NSHVLPALMRRFHEAKANNADEVVVWGSGSPLREFLHVDDLADACVFLMDQYSGFEHVNV 244

Query: 253 GSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNPRISLRDGL 312
           GSG EV+IKELAELVKEVVGF+G LVWD +KPDGTPRKLMDSSKLA LGW P+ISL+DGL
Sbjct: 245 GSGVEVTIKELAELVKEVVGFKGKLVWDTTKPDGTPRKLMDSSKLASLGWTPKISLKDGL 304

Query: 313 VDTYKWYVQNVQQ 326
             TY+WY++NV Q
Sbjct: 305 SQTYEWYLENVVQ 317

[Close]

BLAST of CmaCh05G010180 vs. TAIR 10
Match: AT1G17890.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 5.5e-157
Identity = 263/313 (84.03%), Postives = 284/313 (90.73%), Query Frame = 0


Query: 13  SFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVESFFANEKP 72
           SF+ +KSAKIF+AGHRGLVGSAIVRKLQ  GFTNL+LR+HSELDLT QSDVESFFA EKP
Sbjct: 5   SFMLEKSAKIFVAGHRGLVGSAIVRKLQDQGFTNLVLRTHSELDLTSQSDVESFFATEKP 64

Query: 73  RFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSSCIYPKFAP 132
            + ILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY HGV+KLLFLGSSCIYPKFAP
Sbjct: 65  VYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYTHGVKKLLFLGSSCIYPKFAP 124

Query: 133 QPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYGPNDNFHPE 192
           QPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYR+QH W+AISGMPTNLYG NDNFHPE
Sbjct: 125 QPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRLQHQWDAISGMPTNLYGQNDNFHPE 184

Query: 193 NSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEYSDLGHVNV 252
           NSHVLPALMRRFHEAK N A EVVVWGSGSPLREFLHVDDLADA  FLM++YS   HVNV
Sbjct: 185 NSHVLPALMRRFHEAKANNADEVVVWGSGSPLREFLHVDDLADACVFLMDQYSGFEHVNV 244

Query: 253 GSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNPRISLRDGL 312
           GSG EV+IKELAELVKEVVGF+G LVWD +KPDGTPRKLMDSSKLA LGW P+ISL+DGL
Sbjct: 245 GSGVEVTIKELAELVKEVVGFKGKLVWDTTKPDGTPRKLMDSSKLASLGWTPKISLKDGL 304

Query: 313 VDTYKWYVQNVQQ 326
             TY+WY++NV Q
Sbjct: 305 SQTYEWYLENVVQ 317

[Close]

BLAST of CmaCh05G010180 vs. TAIR 10
Match: AT1G73250.1 (GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase 1 )

HSP 1 Score: 547.0 bits (1408), Expect = 1.0e-155
Identity = 260/319 (81.50%), Postives = 288/319 (90.28%), Query Frame = 0


Query: 5   ADNASSGSSFLSDKSAKIFIAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQSDVE 64
           A+   S  S +SDKSAKIF+AGHRGLVGSAIVRKLQ+ GFTNL+L++H+ELDLTRQ+DVE
Sbjct: 2   AETIGSEVSSMSDKSAKIFVAGHRGLVGSAIVRKLQEQGFTNLVLKTHAELDLTRQADVE 61

Query: 65  SFFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSS 124
           SFF+ EKP + ILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY HGV+KLLFLGSS
Sbjct: 62  SFFSQEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYEHGVKKLLFLGSS 121

Query: 125 CIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLYG 184
           CIYPKFAPQPIPE ALLT  LEPTNEWYA+AKIAGIK CQAYRIQH W+AISGMPTNLYG
Sbjct: 122 CIYPKFAPQPIPESALLTASLEPTNEWYAIAKIAGIKTCQAYRIQHGWDAISGMPTNLYG 181

Query: 185 PNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEEY 244
           PNDNFHPENSHVLPALMRRFHEAKVNGA+EVVVWG+GSPLREFLHVDDLADA  FL++ Y
Sbjct: 182 PNDNFHPENSHVLPALMRRFHEAKVNGAEEVVVWGTGSPLREFLHVDDLADACVFLLDRY 241

Query: 245 SDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNP 304
           S L HVN+GSG+EV+I+ELAELVKEVVGFEG L WD +KPDGTPRKLMDSSKLA LGW P
Sbjct: 242 SGLEHVNIGSGQEVTIRELAELVKEVVGFEGKLGWDCTKPDGTPRKLMDSSKLASLGWTP 301

Query: 305 RISLRDGLVDTYKWYVQNV 324
           ++SLRDGL  TY WY++NV
Sbjct: 302 KVSLRDGLSQTYDWYLKNV 320

[Close]

BLAST of CmaCh05G010180 vs. TAIR 10
Match: AT5G59290.1 (UDP-glucuronic acid decarboxylase 3 )

HSP 1 Score: 80.5 bits (197), Expect = 2.8e-15
Identity = 82/310 (26.45%), Postives = 129/310 (41.61%), Query Frame = 0


Query: 21  KIFIAGHRGLVGSAIVRKLQQLGFTNLLLR---------------SHSELDLTRQSDVES 80
           +I I+G  G +GS +V KL +     +++                 H   +L R  DV  
Sbjct: 31  RILISGGAGFIGSHLVDKLMENEKNEVVVADNYFTGSKENLKKWIGHPRFELIRH-DVTE 90

Query: 81  FFANEKPRFAILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRHGVQKLLFLGSSC 140
               E  R   LA       +  N  P   I  N+    N++  A R G  ++L   +S 
Sbjct: 91  PLLIEVDRIYHLACPASPIFYKYN--PVKTIKTNVIGTLNMLGLAKRVGA-RILLTSTSE 150

Query: 141 IYPKFAPQPIPEDALLTGPLEP--TNEWYAVAKIAGIKMCQAYRIQHNWNAISGMPTNLY 200
           +Y      P PE     G + P      Y   K     +   Y  QH          N Y
Sbjct: 151 VYGDPLIHPQPES--YWGNVNPIGVRSCYDEGKRVAETLMFDYHRQHGIEIRIARIFNTY 210

Query: 201 GPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGSGSPLREFLHVDDLADAVAFLMEE 260
           GP  N   ++  V+   +     A+    + + V   G+  R F +V D+ D +  LME 
Sbjct: 211 GPRMNI--DDGRVVSNFI-----AQALRGEALTVQKPGTQTRSFCYVSDMVDGLIRLMEG 270

Query: 261 YSDLGHVNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAE-LGW 313
            +D G +N+G+  E ++ ELAE VKE++    ++   ++ PD   ++  D SK  E LGW
Sbjct: 271 -NDTGPINIGNPGEFTMVELAETVKELINPSIEIKMVENTPDDPRQRKPDISKAKEVLGW 326

The following BLAST results are available for this feature:

BLAST of CmaCh05G010180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU0	4.6e-156	82.41	Putative GDP-L-fucose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=GER2 PE=1 SV...	[more]
O49213	1.5e-154	81.50	GDP-L-fucose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=GER1 PE=1 SV=3	[more]
Q67WR2	6.0e-148	77.33	Probable GDP-L-fucose synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q67WR5	6.0e-108	58.81	Putative GDP-L-fucose synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
P55353	1.0e-102	58.42	GDP-L-fucose synthase OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=3...	[more]

BLAST of CmaCh05G010180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17890.1	3.2e-157	82.41	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G17890.2	5.5e-157	84.03	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G17890.3	5.5e-157	84.03	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G73250.1	1.0e-155	81.50	GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase 1	[more]
AT5G59290.1	2.8e-15	26.45	UDP-glucuronic acid decarboxylase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 23..306 e-value: 2.6E-125 score: 419.9
None	No IPR available	GENE3D	3.90.25.10		coord: 184..319 e-value: 2.6E-125 score: 419.9
None	No IPR available	PANTHER	PTHR43238:SF5	GDP-L-FUCOSE SYNTHASE 2-RELATED	coord: 8..325
None	No IPR available	PANTHER	PTHR43238	GDP-L-FUCOSE SYNTHASE	coord: 8..325
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 22..253 e-value: 2.4E-73 score: 246.5
IPR028614	GDP-L-fucose synthase/GDP-L-colitose synthase	HAMAP	MF_00956	GDP_fucose_synth	coord: 19..321 score: 50.885529
IPR028614	GDP-L-fucose synthase/GDP-L-colitose synthase	CDD	cd05239	GDP_FS_SDR_e	coord: 21..319 e-value: 0.0 score: 537.935
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 18..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh05G010180.1	CmaCh05G010180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042351	'de novo' GDP-L-fucose biosynthetic process
biological_process	GO:0009226	nucleotide-sugar biosynthetic process
molecular_function	GO:0050577	GDP-L-fucose synthase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity