CmaCh05G007310 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh05G007310
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	cytochrome P450 CYP73A100-like
Location	Cma_Chr05: 4119909 .. 4121761 (-)
RNA-Seq Expression	CmaCh05G007310
Synteny	CmaCh05G007310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTAGACATGGCTTCCACTGCCTTCCTTTCTCTCTCTGCAATGCTCCTTGTTTCTCTTGTGACCAAATTCATATTCCCCATGTCTCCAATTGCTTTGGGGGTCACCATCTTCCTTGCTCCTCTTCTTACTTACATCATCTCTTCCATGGCCTCCTCCTCCTCCTCCAAGCTTCCTCCTGGCCCTCTCTCCTTTCCCATCTTTGGCAATTGGCTCCAAGTTGGCAACGACCTCAACCATCGCCTTCTCGCTTCCTTGTGCCAAAGATTCGGCTCCATATTCCTCTTGAAGCTCGGCTCCAAGAACCTCGTTGTCGTGTCGGATGCCGAGCTTGCTAGCCAAGTTCTCCATGCTCAGGGCGTTGAGTTTGGCTCTCGTCCTCGAAACGTCGTGTTCGATATCTTCACTGGAAATGGTCAGGATATGGTGTTTACCGTTTATGGGGACCATTGGCGTAAGATGCGTAGAATCATGACGTTGCCGTTCTTCACGAACAAGGTGGTGCATAACTATAGTGGCATGTGGGAGGATGAGATGGACTCGGTGGTTCGTGATCTAAGGAGTAACGACAAGTTTCAATCCGAGGGGATAGTTATTAGAAAGCGTTTGCAGTTGATGCTGTACAATATCATGTATAGAATGATGTTTGATGCGAAGTTTGAATCCCAAGACGATTCTTTGTTCATTGAAGCGACTCGATTCAACTCGGAGAGGAGTCGATTGGCGCAGAGCTTTGAGTACAACTATGGAGATTTCATTCCTTTGCTTAGACCCTTCTTGCGAGGTTATTTGAACAAGTGCAGGGATTTGCAGGGTAGGAGGTTGGAGTTTTTCAACAAAAACTACGTCGAGAAGCGAAGGTAATAGCAAGTCATTATATAATTATGCACAAGTTTTCATTCAATTGTAACTAACATGATTGAATTTATCAATTTTAACAGGAACATTATGGCTGCCAATGGAGATAAGCACAACATAAGTTGTGCTATGGATCATATCATAGACGCTCAGTTGAAAGGCGAGATAAGTGAAGAGAATGTGATTTACATCGTAGAGAACATCAACGTGGCAGCGATAGAAACGACATTGTGGTCAATGGAATGGGCAATAGCGGAGTTGGTGAATCATCCAGACATCCAACACAAGATTCGGGAGGAGCTAAAAAATGTCCTAAAGGGAAACGAAGTGAGTGAATCCAACCTTCATGAACTGCCGTACTTGCAAGCAACAGTGAAAGAGACGCTGAGGCTGCACACTCCAATACCGTTGTTGGTGCCACATATGAACTTGGAAGAGGCGAAACTCGGAGGGTACACCATCCCAAAGGAGTCCAAGGTGGTGGTGAATGCATGGTGGCTCGCAAACAACCCCGAGTGGTGGAAGAACCCAGAAGAGTTCAGGCCAGAGAGGTTCTTTGAGGAAGAAAGTGGGACGGAAGCTGTTGCTGCAGGCAAGGTGGATTATCGGTTCTTACCATTTGGAGTTGGAAGGAGGAGTTGCCCTGGAATTGTGTTGGCAATGCCAATCTTAGGGCTCATCGTTGCCAAATTGGTATCCAAGTTTGAAATGAAGCCTCCAAGTGGGATGGAGAAGATCGATGTGACCGAGAAAGGAGGTCAATTCAGTTTGCACATTGCCAACCACTCCACTGTTGTGTTTAACCCCATAATTGCTTAACTTGCCAACACCCAGTCTCCCTATTTTATATTGGTTTTTCTTTACATATAAAACATTTAATTTGTATTTGAACTCGATCGAATAAATCCTTTGACTAGGAATGATAATGAAGAACACGTTTGATAGAATATCATTTCTCCCATATAGGAACGAACTCATTGTATCGAAGGTCTACCC

mRNA sequence

CTCTAGACATGGCTTCCACTGCCTTCCTTTCTCTCTCTGCAATGCTCCTTGTTTCTCTTGTGACCAAATTCATATTCCCCATGTCTCCAATTGCTTTGGGGGTCACCATCTTCCTTGCTCCTCTTCTTACTTACATCATCTCTTCCATGGCCTCCTCCTCCTCCTCCAAGCTTCCTCCTGGCCCTCTCTCCTTTCCCATCTTTGGCAATTGGCTCCAAGTTGGCAACGACCTCAACCATCGCCTTCTCGCTTCCTTGTGCCAAAGATTCGGCTCCATATTCCTCTTGAAGCTCGGCTCCAAGAACCTCGTTGTCGTGTCGGATGCCGAGCTTGCTAGCCAAGTTCTCCATGCTCAGGGCGTTGAGTTTGGCTCTCGTCCTCGAAACGTCGTGTTCGATATCTTCACTGGAAATGGTCAGGATATGGTGTTTACCGTTTATGGGGACCATTGGCGTAAGATGCGTAGAATCATGACGTTGCCGTTCTTCACGAACAAGGTGGTGCATAACTATAGTGGCATGTGGGAGGATGAGATGGACTCGGTGGTTCGTGATCTAAGGAGTAACGACAAGTTTCAATCCGAGGGGATAGTTATTAGAAAGCGTTTGCAGTTGATGCTGTACAATATCATGTATAGAATGATGTTTGATGCGAAGTTTGAATCCCAAGACGATTCTTTGTTCATTGAAGCGACTCGATTCAACTCGGAGAGGAGTCGATTGGCGCAGAGCTTTGAGTACAACTATGGAGATTTCATTCCTTTGCTTAGACCCTTCTTGCGAGGTTATTTGAACAAGTGCAGGGATTTGCAGGGTAGGAGGTTGGAGTTTTTCAACAAAAACTACGTCGAGAAGCGAAGGAACATTATGGCTGCCAATGGAGATAAGCACAACATAAGTTGTGCTATGGATCATATCATAGACGCTCAGTTGAAAGGCGAGATAAGTGAAGAGAATGTGATTTACATCGTAGAGAACATCAACGTGGCAGCGATAGAAACGACATTGTGGTCAATGGAATGGGCAATAGCGGAGTTGGTGAATCATCCAGACATCCAACACAAGATTCGGGAGGAGCTAAAAAATGTCCTAAAGGGAAACGAAGTGAGTGAATCCAACCTTCATGAACTGCCGTACTTGCAAGCAACAGTGAAAGAGACGCTGAGGCTGCACACTCCAATACCGTTGTTGGTGCCACATATGAACTTGGAAGAGGCGAAACTCGGAGGGTACACCATCCCAAAGGAGTCCAAGGTGGTGGTGAATGCATGGTGGCTCGCAAACAACCCCGAGTGGTGGAAGAACCCAGAAGAGTTCAGGCCAGAGAGGTTCTTTGAGGAAGAAAGTGGGACGGAAGCTGTTGCTGCAGGCAAGGTGGATTATCGGTTCTTACCATTTGGAGTTGGAAGGAGGAGTTGCCCTGGAATTGTGTTGGCAATGCCAATCTTAGGGCTCATCGTTGCCAAATTGGTATCCAAGTTTGAAATGAAGCCTCCAAGTGGGATGGAGAAGATCGATGTGACCGAGAAAGGAGGTCAATTCAGTTTGCACATTGCCAACCACTCCACTGTTGTGTTTAACCCCATAATTGCTTAACTTGCCAACACCCAGTCTCCCTATTTTATATTGGTTTTTCTTTACATATAAAACATTTAATTTGTATTTGAACTCGATCGAATAAATCCTTTGACTAGGAATGATAATGAAGAACACGTTTGATAGAATATCATTTCTCCCATATAGGAACGAACTCATTGTATCGAAGGTCTACCC

Coding sequence (CDS)

ATGGCTTCCACTGCCTTCCTTTCTCTCTCTGCAATGCTCCTTGTTTCTCTTGTGACCAAATTCATATTCCCCATGTCTCCAATTGCTTTGGGGGTCACCATCTTCCTTGCTCCTCTTCTTACTTACATCATCTCTTCCATGGCCTCCTCCTCCTCCTCCAAGCTTCCTCCTGGCCCTCTCTCCTTTCCCATCTTTGGCAATTGGCTCCAAGTTGGCAACGACCTCAACCATCGCCTTCTCGCTTCCTTGTGCCAAAGATTCGGCTCCATATTCCTCTTGAAGCTCGGCTCCAAGAACCTCGTTGTCGTGTCGGATGCCGAGCTTGCTAGCCAAGTTCTCCATGCTCAGGGCGTTGAGTTTGGCTCTCGTCCTCGAAACGTCGTGTTCGATATCTTCACTGGAAATGGTCAGGATATGGTGTTTACCGTTTATGGGGACCATTGGCGTAAGATGCGTAGAATCATGACGTTGCCGTTCTTCACGAACAAGGTGGTGCATAACTATAGTGGCATGTGGGAGGATGAGATGGACTCGGTGGTTCGTGATCTAAGGAGTAACGACAAGTTTCAATCCGAGGGGATAGTTATTAGAAAGCGTTTGCAGTTGATGCTGTACAATATCATGTATAGAATGATGTTTGATGCGAAGTTTGAATCCCAAGACGATTCTTTGTTCATTGAAGCGACTCGATTCAACTCGGAGAGGAGTCGATTGGCGCAGAGCTTTGAGTACAACTATGGAGATTTCATTCCTTTGCTTAGACCCTTCTTGCGAGGTTATTTGAACAAGTGCAGGGATTTGCAGGGTAGGAGGTTGGAGTTTTTCAACAAAAACTACGTCGAGAAGCGAAGGAACATTATGGCTGCCAATGGAGATAAGCACAACATAAGTTGTGCTATGGATCATATCATAGACGCTCAGTTGAAAGGCGAGATAAGTGAAGAGAATGTGATTTACATCGTAGAGAACATCAACGTGGCAGCGATAGAAACGACATTGTGGTCAATGGAATGGGCAATAGCGGAGTTGGTGAATCATCCAGACATCCAACACAAGATTCGGGAGGAGCTAAAAAATGTCCTAAAGGGAAACGAAGTGAGTGAATCCAACCTTCATGAACTGCCGTACTTGCAAGCAACAGTGAAAGAGACGCTGAGGCTGCACACTCCAATACCGTTGTTGGTGCCACATATGAACTTGGAAGAGGCGAAACTCGGAGGGTACACCATCCCAAAGGAGTCCAAGGTGGTGGTGAATGCATGGTGGCTCGCAAACAACCCCGAGTGGTGGAAGAACCCAGAAGAGTTCAGGCCAGAGAGGTTCTTTGAGGAAGAAAGTGGGACGGAAGCTGTTGCTGCAGGCAAGGTGGATTATCGGTTCTTACCATTTGGAGTTGGAAGGAGGAGTTGCCCTGGAATTGTGTTGGCAATGCCAATCTTAGGGCTCATCGTTGCCAAATTGGTATCCAAGTTTGAAATGAAGCCTCCAAGTGGGATGGAGAAGATCGATGTGACCGAGAAAGGAGGTCAATTCAGTTTGCACATTGCCAACCACTCCACTGTTGTGTTTAACCCCATAATTGCTTAA

Protein sequence

MASTAFLSLSAMLLVSLVTKFIFPMSPIALGVTIFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLCQRFGSIFLLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKMRRIMTLPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLMLYNIMYRMMFDAKFESQDDSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRRLEFFNKNYVEKRRNIMAANGDKHNISCAMDHIIDAQLKGEISEENVIYIVENINVAAIETTLWSMEWAIAELVNHPDIQHKIREELKNVLKGNEVSESNLHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEEFRPERFFEEESGTEAVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKPPSGMEKIDVTEKGGQFSLHIANHSTVVFNPIIA

Homology

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BLAST of CmaCh05G007310 vs. ExPASy Swiss-Prot
Match: H2DH22 (Cytochrome P450 CYP73A100 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 4.1e-231
Identity = 401/530 (75.66%), Postives = 453/530 (85.47%), Query Frame = 0


Query: 1   MASTAFLSLSAMLLVSLV-TKFIFPMSPIALGVTIFLAPLLTYIISSMASSSSSKLPPGP 60
           +A++   +++   L  L+ + +    +P  L + I +  +L +I+  ++S S S LPPGP
Sbjct: 5   LANSIIFTIALFSLAKLLCSPYSINFTPSQLVLPIAIIGILPFILILLSSISKSNLPPGP 64

Query: 61  LSFPIFGNWLQVGNDLNHRLLASLCQRFGSIFLLKLGSKNLVVVSDAELASQVLHAQGVE 120
           LS PIFGNWLQVGNDLNHRLLA++ + +G +FLLKLGSKNL VVSD ELA+QVLH QGVE
Sbjct: 65  LSLPIFGNWLQVGNDLNHRLLAAMSKTYGPVFLLKLGSKNLAVVSDPELANQVLHTQGVE 124

Query: 121 FGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKMRRIMTLPFFTNKVVHNYSGMWEDEMDSV 180
           FGSRPRNVVFDIFTGNGQDMVFT+YG+HWRKMRRIMTLPFFTNKVVH YS MWE+EMD V
Sbjct: 125 FGSRPRNVVFDIFTGNGQDMVFTIYGEHWRKMRRIMTLPFFTNKVVHQYSNMWEEEMDLV 184

Query: 181 VRDLRSND-KFQSEGIVIRKRLQLMLYNIMYRMMFDAKFESQDDSLFIEATRFNSERSRL 240
           V DLR+     + EGIVIRKRLQLMLYNIMYRMMFDAKFESQDD LFIEATRFNSERSRL
Sbjct: 185 VHDLRNGKAAAREEGIVIRKRLQLMLYNIMYRMMFDAKFESQDDPLFIEATRFNSERSRL 244

Query: 241 AQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRRLEFFNKNYVEKRRNIMAANGDKHNISC 300
           AQSF+YNYGDFIPLLRPFLRGYLNKCRDLQ RRL FFN  YVEKRR IMAANG+KH I C
Sbjct: 245 AQSFDYNYGDFIPLLRPFLRGYLNKCRDLQNRRLAFFNNYYVEKRRKIMAANGEKHKIIC 304

Query: 301 AMDHIIDAQLKGEISEENVIYIVENINVAAIETTLWSMEWAIAELVNHPDIQHKIREELK 360
           AMDHIIDAQ+ GEIS+ENV+YIVENINVAAIETTLWSMEWAIAELVNHP +Q KIR+E+ 
Sbjct: 305 AMDHIIDAQMMGEISDENVLYIVENINVAAIETTLWSMEWAIAELVNHPTVQRKIRDEIS 364

Query: 361 NVLKGNEVSESNLHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKLGGYTIPKESKVVV 420
            VLKG+ V+E+NLHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKL GYTIPKESKVVV
Sbjct: 365 TVLKGSPVTEANLHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKLAGYTIPKESKVVV 424

Query: 421 NAWWLANNPEWWKNPEEFRPERFFEEESGTEAVAAGKVDYRFLPFGVGRRSCPGIVLAMP 480
           NAWWLANNP WWKNPEEFRPERF EEE G  A    +VD+R+LPFGVGRRSCPG++LA+P
Sbjct: 425 NAWWLANNPAWWKNPEEFRPERFTEEEGG--AAGRVQVDFRYLPFGVGRRSCPGMILALP 484

Query: 481 ILGLIVAKLVSKFEMK-PPSGMEKIDVTEKGGQFSLHIANHSTVVFNPII 528
           ILGL++AKLV  FEM    +G+ KIDV+E+GGQFSLHIA HSTVVFN I+
Sbjct: 485 ILGLVIAKLVMNFEMTVAAAGLGKIDVSEEGGQFSLHIAKHSTVVFNAIM 532

[Close]

BLAST of CmaCh05G007310 vs. ExPASy Swiss-Prot
Match: F1B283 (Trans-cinnamate 4-monooxygenase C4H2 OS=Petunia hybrida OX=4102 GN=C4H2 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.1e-188
Identity = 318/496 (64.11%), Postives = 392/496 (79.03%), Query Frame = 0


Query: 32  VTIFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLCQRFGSIF 91
           + +F+A ++  I+S +  S   KLPPGP+  P+FGNWLQVG+DLNHR L    ++FG +F
Sbjct: 11  IGLFIAIIIATIVSKL-RSKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTEYAKKFGDLF 70

Query: 92  LLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKM 151
           LL++G +NLVVVS  +LA +VLH QGVEFGSR RNVVFDIFTG GQDMVFTVYG+HWRKM
Sbjct: 71  LLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKM 130

Query: 152 RRIMTLPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLMLYNIMYRM 211
           RRIMT+PFFTNKVV  Y G WE E++SVV D++   +  + GIV+RKRLQLM+YN M+R+
Sbjct: 131 RRIMTVPFFTNKVVQQYRGGWEYEVESVVEDVKKMKESNTNGIVLRKRLQLMMYNNMFRI 190

Query: 212 MFDAKFESQDDSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRR 271
           MFD +FES+DD LF++    N ERSRLAQSFEYNYGDFIP+LRPFLRGYL  C++++ +R
Sbjct: 191 MFDRRFESEDDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLKICKEVKEKR 250

Query: 272 LEFFNKNYVEKRRNIMAANG-DKHNISCAMDHIIDAQLKGEISEENVIYIVENINVAAIE 331
           L+ F   +V++R+ +      D + + CA+DHI++AQ KGEI+E+NV+YIVENINVAAIE
Sbjct: 251 LKLFKDYFVDERKKLANTKSMDSNALKCAIDHILEAQQKGEINEDNVLYIVENINVAAIE 310

Query: 332 TTLWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVSESNLHELPYLQATVKETLRLHT 391
           TTLWS+EW IAELVNHP IQ K+R+E+  VL  G +V+E + H+LPYLQA +KETLRL  
Sbjct: 311 TTLWSIEWGIAELVNHPHIQKKLRDEIDTVLGPGVQVTEPDTHKLPYLQAVIKETLRLRM 370

Query: 392 PIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEEFRPERFFEEESGTE 451
            IPLLVPHMNL EAKLGGY IP ESK++VNAWWLANNP  WKNPEEFRPERFFEEE   E
Sbjct: 371 AIPLLVPHMNLHEAKLGGYDIPAESKILVNAWWLANNPAHWKNPEEFRPERFFEEEKHVE 430

Query: 452 AVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKPPSGMEKIDVTEKGG 511
              A   D+R+LPFGVGRRSCPGI+LA+PILG+ + +LV  FE+ PP G  KID TEKGG
Sbjct: 431 ---ANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTTEKGG 490

Query: 512 QFSLHIANHSTVVFNP 526
           QFSLHI  HST+V  P
Sbjct: 491 QFSLHILKHSTIVLKP 502

[Close]

BLAST of CmaCh05G007310 vs. ExPASy Swiss-Prot
Match: Q43240 (Trans-cinnamate 4-monooxygenase OS=Zinnia violacea OX=34245 GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 9.6e-188
Identity = 317/491 (64.56%), Postives = 387/491 (78.82%), Query Frame = 0


Query: 37  APLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLCQRFGSIFLLKLG 96
           A ++  I  S       KLPPGP+  PIFGNWLQVG+DLNHR L  L ++FG IFLL++G
Sbjct: 15  AAIIASIFISKLRGKRFKLPPGPVPVPIFGNWLQVGDDLNHRNLTDLAKKFGEIFLLRMG 74

Query: 97  SKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKMRRIMT 156
            +NLVVVS   LA +VLH QGVEFGSR RNVVFDIFTG GQDMVFTVYG+HWRKMRRIMT
Sbjct: 75  QRNLVVVSSPNLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMT 134

Query: 157 LPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLMLYNIMYRMMFDAK 216
           +PFFTNKVV  Y   WE E  +VV D++ N K  +EG+VIRKRLQLM+YN M+R+MFD +
Sbjct: 135 VPFFTNKVVQQYRTGWEAEAAAVVDDVKKNPKAATEGVVIRKRLQLMMYNNMFRIMFDRR 194

Query: 217 FESQDDSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRRLEFFN 276
           FES+DD LF++    N ERSRLAQSFEYNYGDFIP+LRPFL+GYL  C++++ +R + F 
Sbjct: 195 FESEDDPLFVKLKMLNGERSRLAQSFEYNYGDFIPILRPFLKGYLKLCKEVKEKRFQLFK 254

Query: 277 KNYVEKRRNIMAANG-DKHNISCAMDHIIDAQLKGEISEENVIYIVENINVAAIETTLWS 336
             +V++R+ + +    D + + CA+DHI+DA+ KGEI+E+NV+YIVENINVAAIETTLWS
Sbjct: 255 DYFVDERKKLGSTKSMDNNQLKCAIDHILDAKDKGEINEDNVLYIVENINVAAIETTLWS 314

Query: 337 MEWAIAELVNHPDIQHKIREELKNVL-KGNEVSESNLHELPYLQATVKETLRLHTPIPLL 396
           +EWAIAELVNHP+IQ K+R EL + L  G +V+E +LH+LPYLQA +KETLRL   IPLL
Sbjct: 315 IEWAIAELVNHPEIQAKLRHELVSQLGPGVQVTEPDLHKLPYLQAVIKETLRLRMAIPLL 374

Query: 397 VPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEEFRPERFFEEESGTEAVAAG 456
           VPHMNL +AKLGGY IP ESK++VNAWWLANNP+ WK PEEFRPERF EEES  E   A 
Sbjct: 375 VPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDQWKKPEEFRPERFLEEESKVE---AN 434

Query: 457 KVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKPPSGMEKIDVTEKGGQFSLH 516
             D+R+LPFGVGRRSCPGI+LA+PILG+ + +LV  FE+ PP G +K+D TEKGGQFSLH
Sbjct: 435 GNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQDKVDTTEKGGQFSLH 494

Query: 517 IANHSTVVFNP 526
           I  HST+V  P
Sbjct: 495 ILKHSTIVAKP 502

[Close]

BLAST of CmaCh05G007310 vs. ExPASy Swiss-Prot
Match: P37115 (Trans-cinnamate 4-monooxygenase OS=Vigna radiata var. radiata OX=3916 GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 3.1e-186
Identity = 319/494 (64.57%), Postives = 387/494 (78.34%), Query Frame = 0


Query: 34  IFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLCQRFGSIFLL 93
           +FLA ++  ++S +      KLPPGPL  PIFGNWLQVG+DLNHR L  L +RFG IFLL
Sbjct: 13  LFLAAVVAIVVSKL-RGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTQLAKRFGDIFLL 72

Query: 94  KLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKMRR 153
           ++G +NLVVVS  +LA +VLH QGVEFGSR RNVVFDIFTG GQDMVFTVYG+HWRKMRR
Sbjct: 73  RMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFTVYGEHWRKMRR 132

Query: 154 IMTLPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLMLYNIMYRMMF 213
           IMT+PFFTNKVV  Y   WE E  +VV D+R N      G+VIR+RLQLM+YN MYR+MF
Sbjct: 133 IMTVPFFTNKVVQQYRHGWEAEAAAVVDDVRKNPDAAVSGLVIRRRLQLMMYNNMYRIMF 192

Query: 214 DAKFESQDDSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRRLE 273
           D +FES++D LF      N ERSRLAQSFEYNYGDFIP+LRPFL+GYL  C++++  RL+
Sbjct: 193 DRRFESEEDPLFQRLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLKICKEVKETRLK 252

Query: 274 FFNKNYVEKRRNIMAANG-DKHNISCAMDHIIDAQLKGEISEENVIYIVENINVAAIETT 333
            F   +V++R+NI +    +   + CA+DHI+DA+ KGEI+E+NV+YIVENINVAAIETT
Sbjct: 253 LFKDYFVDERKNIGSTKSTNNEGLKCAIDHILDAEKKGEINEDNVLYIVENINVAAIETT 312

Query: 334 LWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVSESNLHELPYLQATVKETLRLHTPI 393
           LWS+EW IAELVNHP+IQ K+R+E+  VL  G++V+E ++ +LPYLQA VKETLRL   I
Sbjct: 313 LWSIEWGIAELVNHPEIQQKVRDEIDRVLGVGHQVTEPDIQKLPYLQAVVKETLRLRMAI 372

Query: 394 PLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEEFRPERFFEEESGTEAV 453
           PLLVPHMNL +AKLGGY IP ESK++VNAWWLANNP  WK PEEFRPERFFEEES  E  
Sbjct: 373 PLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEESHVE-- 432

Query: 454 AAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKPPSGMEKIDVTEKGGQF 513
            A   D+R+LPFGVGRRSCPGI+LA+PILG+ + +LV  FE+ PP G  +ID +EKGGQF
Sbjct: 433 -ANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGGQF 492

Query: 514 SLHIANHSTVVFNP 526
           SLHI  HSTVV  P
Sbjct: 493 SLHILKHSTVVAKP 502

[Close]

BLAST of CmaCh05G007310 vs. ExPASy Swiss-Prot
Match: F1B282 (Trans-cinnamate 4-monooxygenase C4H1 OS=Petunia hybrida OX=4102 GN=C4H1 PE=2 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 6.9e-186
Identity = 312/497 (62.78%), Postives = 389/497 (78.27%), Query Frame = 0


Query: 32  VTIFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLCQRFGSIF 91
           + +F A ++  ++S +  S   KLPPGP+  P+FGNWLQVG+DLNHR L    ++FG +F
Sbjct: 11  IGLFFAIIIAIVVSKL-RSKKFKLPPGPIPVPVFGNWLQVGDDLNHRNLTEYAKKFGDLF 70

Query: 92  LLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKM 151
           LL++G +NLVVVS  ELA +VLH QGVEFGSR RNVVFDIFTG GQDMVFTVYG+HWRKM
Sbjct: 71  LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKM 130

Query: 152 RRIMTLPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLMLYNIMYRM 211
           RRIMT+PFFTNKVV  Y G WEDE+  V+ D++   +  + GIV+RKRLQLM+YN MYR+
Sbjct: 131 RRIMTVPFFTNKVVQQYRGGWEDEVAHVIDDVKKMPESATNGIVLRKRLQLMMYNNMYRI 190

Query: 212 MFDAKFESQDDSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRR 271
           MFD +FES+DD LF +    N ERSRLAQSFEYNYGDFIP+LRPFLRGYL  C++++ RR
Sbjct: 191 MFDRRFESEDDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLKICKEVKQRR 250

Query: 272 LEFFNKNYVEKRRNIMAA--NGDKHNISCAMDHIIDAQLKGEISEENVIYIVENINVAAI 331
           L+ F   +V++R+ +     + D + + CA+DHI++A+ KGEI+E+NV+YIVENINVAAI
Sbjct: 251 LQLFKDYFVDERKKLSTTTKSMDNNALKCAIDHILEAEQKGEINEDNVLYIVENINVAAI 310

Query: 332 ETTLWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVSESNLHELPYLQATVKETLRLH 391
           ETTLWS+EW IAELVNHP+IQ K+R+E+ +VL  G +++E + H+LPYLQA +KETLRL 
Sbjct: 311 ETTLWSIEWGIAELVNHPEIQKKLRDEIDSVLGPGVQITEPHTHKLPYLQAVIKETLRLR 370

Query: 392 TPIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEEFRPERFFEEESGT 451
             IPLLVPHMNL +AKL GY IP ESK++VNAWWLANNP  WK PEEFRPERFFEEE   
Sbjct: 371 MAIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPATWKRPEEFRPERFFEEEKHV 430

Query: 452 EAVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKPPSGMEKIDVTEKG 511
           E   A   D+R+LPFGVGRRSCPGI+LA+PILG+ + +LV  FE+ PP G  K+D TEKG
Sbjct: 431 E---ANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLDTTEKG 490

Query: 512 GQFSLHIANHSTVVFNP 526
           GQFSLHI  H T+V  P
Sbjct: 491 GQFSLHILKHFTIVMKP 503

[Close]

BLAST of CmaCh05G007310 vs. TAIR 10
Match: AT2G30490.1 (cinnamate-4-hydroxylase )

HSP 1 Score: 647.1 bits (1668), Expect = 1.2e-185
Identity = 314/496 (63.31%), Postives = 385/496 (77.62%), Query Frame = 0


Query: 32  VTIFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLCQRFGSIF 91
           + +F+A +L  +IS +      KLPPGP+  PIFGNWLQVG+DLNHR L    ++FG +F
Sbjct: 11  IAVFVAVILATVISKL-RGKKLKLPPGPIPIPIFGNWLQVGDDLNHRNLVDYAKKFGDLF 70

Query: 92  LLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVYGDHWRKM 151
           LL++G +NLVVVS  +L  +VL  QGVEFGSR RNVVFDIFTG GQDMVFTVYG+HWRKM
Sbjct: 71  LLRMGQRNLVVVSSPDLTKEVLLTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKM 130

Query: 152 RRIMTLPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLMLYNIMYRM 211
           RRIMT+PFFTNKVV      WE E  SVV D++ N    ++GIV+RKRLQLM+YN M+R+
Sbjct: 131 RRIMTVPFFTNKVVQQNREGWEFEAASVVEDVKKNPDSATKGIVLRKRLQLMMYNNMFRI 190

Query: 212 MFDAKFESQDDSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNKCRDLQGRR 271
           MFD +FES+DD LF+     N ERSRLAQSFEYNYGDFIP+LRPFLRGYL  C+D++ RR
Sbjct: 191 MFDRRFESEDDPLFLRLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLKICQDVKDRR 250

Query: 272 LEFFNKNYVEKRRNIMAAN-GDKHNISCAMDHIIDAQLKGEISEENVIYIVENINVAAIE 331
           +  F K +V++R+ I ++       + CA+DHI++A+ KGEI+E+NV+YIVENINVAAIE
Sbjct: 251 IALFKKYFVDERKQIASSKPTGSEGLKCAIDHILEAEQKGEINEDNVLYIVENINVAAIE 310

Query: 332 TTLWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVSESNLHELPYLQATVKETLRLHT 391
           TTLWS+EW IAELVNHP+IQ K+R EL  VL  G +V+E +LH+LPYLQA VKETLRL  
Sbjct: 311 TTLWSIEWGIAELVNHPEIQSKLRNELDTVLGPGVQVTEPDLHKLPYLQAVVKETLRLRM 370

Query: 392 PIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEEFRPERFFEEESGTE 451
            IPLLVPHMNL +AKL GY IP ESK++VNAWWLANNP  WK PEEFRPERFFEEES  E
Sbjct: 371 AIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPNSWKKPEEFRPERFFEEESHVE 430

Query: 452 AVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKPPSGMEKIDVTEKGG 511
              A   D+R++PFGVGRRSCPGI+LA+PILG+ + ++V  FE+ PP G  K+D +EKGG
Sbjct: 431 ---ANGNDFRYVPFGVGRRSCPGIILALPILGITIGRMVQNFELLPPPGQSKVDTSEKGG 490

Query: 512 QFSLHIANHSTVVFNP 526
           QFSLHI NHS +V  P
Sbjct: 491 QFSLHILNHSIIVMKP 502

[Close]

BLAST of CmaCh05G007310 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 247.3 bits (630), Expect = 2.8e-65
Identity = 161/502 (32.07%), Postives = 265/502 (52.79%), Query Frame = 0


Query: 24  PMSPIALGVTIFLAPLLTYIISSMASSSSS-KLPPGPLSFPIFGNWLQVGNDLNHRLLAS 83
           P S + LGV   LA +  + I   + S +   LPPGP   PI GN   + +D+ H    +
Sbjct: 52  PYSSVMLGVAALLAVVCYFWIQGKSKSKNGPPLPPGPWPLPIVGNLPFLNSDVLHTQFQA 111

Query: 84  LCQRFGSIFLLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFT 143
           L  + G +  + LGSK  +VVS  ++A +VL    + F +     V  I T  G+D++++
Sbjct: 112 LTLKHGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWS 171

Query: 144 VYGDHWRKMRRIMTLPFFTNKVVH-NYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQ 203
            YG HWR++R++  +  FT   +  +YS   E+   ++V    S    +   + + +++ 
Sbjct: 172 PYGTHWRRLRKLCVMKMFTTPTLEASYSTRREETRQTIVH--MSEMAREGSPVNLGEQIF 231

Query: 204 LMLYNIMYRMMFDAKFESQDDSLFIEATRFNSERSRLAQSFE-----YNYGDFIPLLRPF 263
           L ++N++ RMM+ A  E        E T   +E   L           NY DF PL   F
Sbjct: 232 LSIFNVVTRMMWGATVEGD------ERTSLGNELKTLISDISDIEGIQNYSDFFPLFSRF 291

Query: 264 --------LRGYLNKCRDLQGRRLEFFNKNYVEKRRNIMAANGDKHNISCAMDHIIDAQL 323
                   ++G++ K   L  R +E   K   +K       + ++ +    +  + D   
Sbjct: 292 DFQGLVKQMKGHVKKLDLLFDRVMESHVKMVGKK-------SEEEEDFLQYLLRVKDDDE 351

Query: 324 KGEISEENVIYIVENINVAAIETTLWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVS 383
           K  +S  +V  ++ ++ +  ++T++ + E+A+AE+V+ P++ +KIR EL  V+ K N V 
Sbjct: 352 KAPLSMTHVKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVE 411

Query: 384 ESNLHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNP 443
           ES+L +LPYLQA +KETLRLH  +PLLVPH N E + + GYT+PK+SK+ +N W +  +P
Sbjct: 412 ESHLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDP 471

Query: 444 EWWKNPEEFRPERFFEEESGTEAVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKL 503
           + W  P EF+PERF E      ++     D+++LPFG GRR C  I +A  ++   +A L
Sbjct: 472 KNWDEPNEFKPERFLE-----NSLDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASL 531

Query: 504 VSKFEMKPPSGMEKIDVTEKGG 510
           +  F+ K P G +K +V EK G
Sbjct: 532 LHSFDWKAPQG-QKFEVEEKFG 532

[Close]

BLAST of CmaCh05G007310 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 244.2 bits (622), Expect = 2.4e-64
Identity = 160/502 (31.87%), Postives = 263/502 (52.39%), Query Frame = 0


Query: 24  PMSPIALGVTIFLAPL-LTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLAS 83
           P S + L V   LA +   +I     S     LPPGP   PI GN   + +D+ H    +
Sbjct: 21  PYSSVMLAVAALLAVVCYLWIQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQA 80

Query: 84  LCQRFGSIFLLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFT 143
           L  ++G +  + LGSK  +VVS  ++A +VL    + F +     V  I T  G+D++++
Sbjct: 81  LTLKYGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWS 140

Query: 144 VYGDHWRKMRRIMTLPFFTNKVVH-NYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQ 203
            YG HWR++R++  +  FT   +  +YS   E+   ++V    S    +   + + +++ 
Sbjct: 141 PYGTHWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVH--MSEMAREGSPVNLGEQIF 200

Query: 204 LMLYNIMYRMMFDAKFESQDDSLFIEATRFNSERSRLAQSFE-----YNYGDFIPLLRPF 263
           L ++N++ RMM+ A  E        E T   +E   L           NY DF PL   F
Sbjct: 201 LSIFNVVTRMMWGATVEGD------ERTSLGNELKTLISDISDIEGIQNYSDFFPLFSRF 260

Query: 264 --------LRGYLNKCRDLQGRRLEFFNKNYVEKRRNIMAANGDKHNISCAMDHIIDAQL 323
                   ++G++ K   L  R +E   K   +K       + ++ +    +  + D   
Sbjct: 261 DFQGLVKQMKGHVKKLDLLFDRVMESHVKMVGKK-------SEEEEDFLQYLLRVKDDDE 320

Query: 324 KGEISEENVIYIVENINVAAIETTLWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVS 383
           K  +S  +V  ++ ++ +  ++T++ + E+A+AE+V+ P++ +KIR EL  V+ K N V 
Sbjct: 321 KAPLSMTHVKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVE 380

Query: 384 ESNLHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNP 443
           ES+L +LPYLQA +KETLRLH  +PLLVPH N E + + GYT+PK+SK+ +N W +  +P
Sbjct: 381 ESHLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDP 440

Query: 444 EWWKNPEEFRPERFFEEESGTEAVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKL 503
           + W  P EF+PERF E      ++     D+++LPFG GRR C  I +A  ++   +A L
Sbjct: 441 KNWDEPNEFKPERFLE-----NSLDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASL 500

Query: 504 VSKFEMKPPSGMEKIDVTEKGG 510
           +  F+ K P G +K +V EK G
Sbjct: 501 LHSFDWKAPQG-QKFEVEEKFG 501

[Close]

BLAST of CmaCh05G007310 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 243.4 bits (620), Expect = 4.0e-64
Identity = 168/501 (33.53%), Postives = 266/501 (53.09%), Query Frame = 0


Query: 21  FIFPMSPIALGVTIFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLL 80
           F+  ++ IA  V+  L   L Y           K PPGP   PI GN   +   +  R  
Sbjct: 4   FLIAVATIAAVVSYKLIQRLRY-----------KFPPGPSPKPIVGNLYDI-KPVRFRCY 63

Query: 81  ASLCQRFGSIFLLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMV 140
               Q +G I  + +GS   VVVS AELA +VL     +   R RN   + F+ NGQD++
Sbjct: 64  YEWAQSYGPIISVWIGSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLI 123

Query: 141 FTVYGDHWRKMRRIMTLPFFTNKVVHNYSGMWEDE----MDSVVRDLRSNDKFQSEGIVI 200
           +  YG H+ K+R++ TL  FT K + +   + EDE    ++SV RD    +  +++G+ +
Sbjct: 124 WADYGPHYVKVRKVCTLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPEN-RAKGLQL 183

Query: 201 RKRLQLMLYNIMYRMMFDAKFESQDDSLFIEATRFNSERSR-LAQSFEYNYGDFIPLLRP 260
           RK L  + +N + R+ F  +F + +  +  +   F +  S  L      +  + IP LR 
Sbjct: 184 RKYLGAVAFNNITRLAFGKRFMNAEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLR- 243

Query: 261 FLRGYLNKCRDLQGRRLEFFNKNYVEKRRNIMAANGDKHNISCAMDHIIDAQL----KGE 320
           ++     K     G R +   +  +E+  + +A    +   S A  H +DA L    + +
Sbjct: 244 WMFPADEKAFAEHGARRDRLTRAIMEE--HTLA----RQKSSGAKQHFVDALLTLKDQYD 303

Query: 321 ISEENVIYIVENINVAAIETTLWSMEWAIAELVNHPDIQHKIREELKNVLKGNEV-SESN 380
           +SE+ +I ++ ++  A ++TT  + EWA+AE++ +P +Q K++EE   V+  + + +E++
Sbjct: 304 LSEDTIIGLLWDMITAGMDTTAITAEWAMAEMIKNPRVQQKVQEEFDRVVGLDRILTEAD 363

Query: 381 LHELPYLQATVKETLRLHTPIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWW 440
              LPYLQ  VKE+ RLH P PL++PH +  + K+GGY IPK S V VN W +A +P  W
Sbjct: 364 FSRLPYLQCVVKESFRLHPPTPLMLPHRSNADVKIGGYDIPKGSNVHVNVWAVARDPAVW 423

Query: 441 KNPEEFRPERFFEEESGTEAVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSK 500
           KNP EFRPERF EE+     V     D+R LPFG GRR CPG  L + ++  +++ L+  
Sbjct: 424 KNPFEFRPERFLEED-----VDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHH 479

Query: 501 FEMKPPSGM--EKIDVTEKGG 510
           F   PP G   E+ID++E  G
Sbjct: 484 FVWTPPQGTKPEEIDMSENPG 479

[Close]

BLAST of CmaCh05G007310 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 232.3 bits (591), Expect = 9.2e-61
Identity = 161/494 (32.59%), Postives = 258/494 (52.23%), Query Frame = 0


Query: 25  MSPIALGVTIFLAPLLTYIISSMASSSSSKLPPGPLSFPIFGNWLQVGNDLNHRLLASLC 84
           ++P A+ +   +  +L YI      S    LPPGP   PI GN   +  DL H   A+L 
Sbjct: 15  LTPYAIVILTTVFSILWYIFK---RSPQPSLPPGPRGLPIVGNLPFLDPDL-HTYFANLA 74

Query: 85  QRFGSIFLLKLGSKNLVVVSDAELASQVLHAQGVEFGSRPRNVVFDIFTGNGQDMVFTVY 144
           Q  G IF L LGSK  +VV+   LA ++L  Q + F +R   +     T  G D+V+T Y
Sbjct: 75  QSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPY 134

Query: 145 GDHWRKMRRIMTLPFFTNKVVHNYSGMWEDEMDSVVRDLRSNDKFQSEGIVIRKRLQLML 204
           G  WR++R+I  L   + K + ++  +   E+    R L    + QS  + +  +L L +
Sbjct: 135 GAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSP-VKVGDQLFLTM 194

Query: 205 YNIMYRMMFDAKFESQD-DSLFIEATRFNSERSRLAQSFEYNYGDFIPLLRPFLRGYLNK 264
            N+   M++    ++++ +S+  E     SE +RL    E N  DF P L  F    L K
Sbjct: 195 MNLTMNMLWGGSVKAEEMESVGTEFKGVISEITRLLS--EPNVSDFFPWLARFDLQGLVK 254

Query: 265 CRDLQGRRLEFFNKNYVEKRRNIMAANGDKHNISCAMDHIIDAQLKGEISEENVIYIVEN 324
              +  R L+      +E+ + +   + D+  +   + +++  +LK +  +  V   + +
Sbjct: 255 RMGVCARELDAVLDRAIEQMKPLRGRDDDE--VKDFLQYLM--KLKDQEGDSEVPITINH 314

Query: 325 IN-------VAAIETTLWSMEWAIAELVNHPDIQHKIREELKNVL-KGNEVSESNLHELP 384
           +        V   +T+  ++E+A+AEL+++P++  + +EEL  V+ K N V ES++  LP
Sbjct: 315 VKALLTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLP 374

Query: 385 YLQATVKETLRLHTPIPLLVPHMNLEEAKLGGYTIPKESKVVVNAWWLANNPEWWKNPEE 444
           Y+ A +KETLRLH  +PLLVPH   E   +GGYTIPK++K+ VN W +  +P  W+NP E
Sbjct: 375 YILAIMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTE 434

Query: 445 FRPERFFEEESGTEAVAAGKVDYRFLPFGVGRRSCPGIVLAMPILGLIVAKLVSKFEMKP 504
           FRPERF +  S     A    +Y + PFG GRR C G+ LA  ++   +A L+  F+ K 
Sbjct: 435 FRPERFLDNNSCDFTGA----NYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKI 492

Query: 505 PSGMEKIDVTEKGG 510
           P G   +D+ EK G
Sbjct: 495 PEG-HVLDLKEKFG 492

The following BLAST results are available for this feature:

BLAST of CmaCh05G007310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH22	4.1e-231	75.66	Cytochrome P450 CYP73A100 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
F1B283	1.1e-188	64.11	Trans-cinnamate 4-monooxygenase C4H2 OS=Petunia hybrida OX=4102 GN=C4H2 PE=2 SV=...	[more]
Q43240	9.6e-188	64.56	Trans-cinnamate 4-monooxygenase OS=Zinnia violacea OX=34245 GN=CYP73A12 PE=2 SV=...	[more]
P37115	3.1e-186	64.57	Trans-cinnamate 4-monooxygenase OS=Vigna radiata var. radiata OX=3916 GN=CYP73A2...	[more]
F1B282	6.9e-186	62.78	Trans-cinnamate 4-monooxygenase C4H1 OS=Petunia hybrida OX=4102 GN=C4H1 PE=2 SV=...	[more]

BLAST of CmaCh05G007310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30490.1	1.2e-185	63.31	cinnamate-4-hydroxylase	[more]
AT4G22690.1	2.8e-65	32.07	cytochrome P450, family 706, subfamily A, polypeptide 1	[more]
AT4G22710.1	2.4e-64	31.87	cytochrome P450, family 706, subfamily A, polypeptide 2	[more]
AT2G40890.1	4.0e-64	33.53	cytochrome P450, family 98, subfamily A, polypeptide 3	[more]
AT4G12300.1	9.2e-61	32.59	cytochrome P450, family 706, subfamily A, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 470..493 score: 31.87 coord: 108..129 score: 29.24 coord: 316..333 score: 29.64 coord: 460..470 score: 59.77 coord: 336..362 score: 32.8 coord: 203..221 score: 23.23 coord: 419..443 score: 37.24 coord: 378..396 score: 38.08 coord: 84..103 score: 35.27
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 461..470 score: 60.86 coord: 327..344 score: 30.69 coord: 379..390 score: 41.12
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 56..517 e-value: 4.1E-108 score: 362.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 48..523 e-value: 7.4E-113 score: 379.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 56..510
None	No IPR available	PIRSR	PIRSR000209-1	PIRSR000209-1	coord: 57..497 e-value: 6.4E-36 score: 121.2
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 39..493 e-value: 6.7E-21 score: 72.6
None	No IPR available	PANTHER	PTHR47948:SF3	OS02G0467000 PROTEIN	coord: 6..527
None	No IPR available	PANTHER	PTHR47948	TRANS-CINNAMATE 4-MONOOXYGENASE	coord: 6..527
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 463..472

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh05G007310.1	CmaCh05G007310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen