CmaCh05G006110 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G006110
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptiontRNA (guanine-N(7)-)-methyltransferase
LocationCma_Chr05: 3066640 .. 3067398 (-)
RNA-Seq ExpressionCmaCh05G006110
SyntenyCmaCh05G006110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGACAACGATGCTAATCCAACTTTCAGCAAGTCAACTGGCCTGCCACGAAAGCGCTTCTACCGTGCAAGAGCACACAGCAACCCTCTAAGTGACTCTCACTTCCCAGTACCAATCTCCCCCAATGAAATTGATTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGACCAGCGAGATAGAACCAAAAAGATTGAATTTGCTGATATTGGTTGCGGTTTTGGTGGTCTTCTGATTAGTCTTTCAACCCTTTTCCCGGAAGCCCTGATGATTGGAATGGAACTCCGGGATAAAGTATCGGAGTACGTTAAGGAACGGATCTTAGCTTTGAGAGTAGCAAATCCTGGCCAATACCAAAACATTTCAGTAGTTCGAACAAATTCCATGAAGTACATTCCAAACTACTTCGAAAAAGCACAACTTTCAAAGATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCACCGCCGGCGTGTAATCAGTCCATTTTTGCTTGATGAGTATGCATATGCGCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGGCTTGTCTAGAGAATCACCCTTGGTTTGAAGCTTTAACATTGGAAGAGCTCGAGGCAGATCCAGCCGTGGAGCTTCTAAGTTCTGCAACGGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACGGACCTTTCAGGCAGTTTATAGGCGCGTTGCTGCAGCACCATGA

mRNA sequence

ATGTTGGACAACGATGCTAATCCAACTTTCAGCAAGTCAACTGGCCTGCCACGAAAGCGCTTCTACCGTGCAAGAGCACACAGCAACCCTCTAAGTGACTCTCACTTCCCAGTACCAATCTCCCCCAATGAAATTGATTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGACCAGCGAGATAGAACCAAAAAGATTGAATTTGCTGATATTGGTTGCGGTTTTGGTGGTCTTCTGATTAGTCTTTCAACCCTTTTCCCGGAAGCCCTGATGATTGGAATGGAACTCCGGGATAAAGTATCGGAGTACGTTAAGGAACGGATCTTAGCTTTGAGAGTAGCAAATCCTGGCCAATACCAAAACATTTCAGTAGTTCGAACAAATTCCATGAAGTACATTCCAAACTACTTCGAAAAAGCACAACTTTCAAAGATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCACCGCCGGCGTGTAATCAGTCCATTTTTGCTTGATGAGTATGCATATGCGCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGGCTTGTCTAGAGAATCACCCTTGGTTTGAAGCTTTAACATTGGAAGAGCTCGAGGCAGATCCAGCCGTGGAGCTTCTAAGTTCTGCAACGGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACGGACCTTTCAGGCAGTTTATAGGCGCGTTGCTGCAGCACCATGA

Coding sequence (CDS)

ATGTTGGACAACGATGCTAATCCAACTTTCAGCAAGTCAACTGGCCTGCCACGAAAGCGCTTCTACCGTGCAAGAGCACACAGCAACCCTCTAAGTGACTCTCACTTCCCAGTACCAATCTCCCCCAATGAAATTGATTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGACCAGCGAGATAGAACCAAAAAGATTGAATTTGCTGATATTGGTTGCGGTTTTGGTGGTCTTCTGATTAGTCTTTCAACCCTTTTCCCGGAAGCCCTGATGATTGGAATGGAACTCCGGGATAAAGTATCGGAGTACGTTAAGGAACGGATCTTAGCTTTGAGAGTAGCAAATCCTGGCCAATACCAAAACATTTCAGTAGTTCGAACAAATTCCATGAAGTACATTCCAAACTACTTCGAAAAAGCACAACTTTCAAAGATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCACCGCCGGCGTGTAATCAGTCCATTTTTGCTTGATGAGTATGCATATGCGCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGGCTTGTCTAGAGAATCACCCTTGGTTTGAAGCTTTAACATTGGAAGAGCTCGAGGCAGATCCAGCCGTGGAGCTTCTAAGTTCTGCAACGGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACGGACCTTTCAGGCAGTTTATAGGCGCGTTGCTGCAGCACCATGA

Protein sequence

MLDNDANPTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDRTKKIEFADIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVEELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVAAAP
Homology
BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: Q8GXB7 (tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24840 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-109
Identity = 197/250 (78.80%), Postives = 221/250 (88.40%), Query Frame = 0

Query: 2   LDNDAN-PTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQ 61
           +DN+    TFSKSTGLPRKRFYRARAHSNPLSDSHFP+PISP  +DYSLH+P+   ++++
Sbjct: 1   MDNETKATTFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNK 60

Query: 62  RDRTKKIEFADIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILAL-RVANPGQ 121
               KK+EFADIGCGFGGLLISL+TLFP+ LMIGMELRDKV+EYVKERILAL R ++ GQ
Sbjct: 61  --FIKKVEFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQ 120

Query: 122 YQNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRV 181
           Y+NISVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY LR 
Sbjct: 121 YENISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRA 180

Query: 182 GGIIYTITDVEELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGR 241
           GGIIYTITDVEELGEWMK+CLE HP FE+LT EEL +DP VELL SATEEGQKVARN G+
Sbjct: 181 GGIIYTITDVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQ 240

Query: 242 TFQAVYRRVA 250
           TF+AV+RR+A
Sbjct: 241 TFRAVFRRIA 248

BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: A2YB34 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_22311 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 2.8e-105
Identity = 184/239 (76.99%), Postives = 208/239 (87.03%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDR-----TKKIEFA 75
           LPRKRFYRARAHSNPLSDSHFP+PISP+E+D S HYP+ FPS +   R       +I FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 76  DIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNS 135
           D+GCGFGGLL+ LSTLFP+ LMIGMELRDKV+EYVKERILALR +NPG+Y NISVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVE 195
           MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 196 ELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVA 250
           ELGEWM++CLE HP FEA+  EE +ADP V+LLS+ATEEGQKVARN G+TFQA++RR++
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: Q67V78 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0236900 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 2.8e-105
Identity = 184/239 (76.99%), Postives = 208/239 (87.03%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDR-----TKKIEFA 75
           LPRKRFYRARAHSNPLSDSHFP+PISP+E+D S HYP+ FPS +   R       +I FA
Sbjct: 18  LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77

Query: 76  DIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNS 135
           D+GCGFGGLL+ LSTLFP+ LMIGMELRDKV+EYVKERILALR +NPG+Y NISVVRTNS
Sbjct: 78  DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVE 195
           MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197

Query: 196 ELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVA 250
           ELGEWM++CLE HP FEA+  EE +ADP V+LLS+ATEEGQKVARN G+TFQA++RR++
Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256

BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: B6SHG7 (tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 5.0e-102
Identity = 180/239 (75.31%), Postives = 204/239 (85.36%), Query Frame = 0

Query: 16  LPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRD-----RTKKIEFA 75
           LPRKRFYRARAHSNPLSDSHFPVPISP E+D S HYP+ FP++   D       ++I FA
Sbjct: 13  LPRKRFYRARAHSNPLSDSHFPVPISPEEVDLSQHYPRYFPADKGSDGEEAAAPQQIRFA 72

Query: 76  DIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNS 135
           D+GCGFGGLL+ LS LFP+ LMIGMELRDKV+EYVKERILALR ANPGQY NISVVRTNS
Sbjct: 73  DVGCGFGGLLVGLSPLFPDTLMIGMELRDKVTEYVKERILALRGANPGQYDNISVVRTNS 132

Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVE 195
           MKYIPNYF KAQL+KMFFLFPDPHFKEKNHRRRVIS  LLDEYAY + VGGIIYTITDVE
Sbjct: 133 MKYIPNYFRKAQLTKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 192

Query: 196 ELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVA 250
           ELGEWM++CLE HP FE +  EE++ADP  +LLS+ATEE QKVARN G+TF A++RR++
Sbjct: 193 ELGEWMRSCLEKHPLFETVPEEEIKADPVFKLLSTATEESQKVARNGGQTFYAIFRRIS 251

BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: A9UMM1 (tRNA (guanine-N(7)-)-methyltransferase B OS=Xenopus tropicalis OX=8364 GN=mettl1-B PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.3e-75
Identity = 133/236 (56.36%), Postives = 179/236 (75.85%), Query Frame = 0

Query: 13  STGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDRTKKIEFADI 72
           S  +P+KR+YR RAHSNP++D  F  P+ P ++D+S  YPQ FP  ++     ++EFADI
Sbjct: 2   SVCMPQKRYYRQRAHSNPMADHTFQYPVCPEQMDWSPLYPQYFPQQEEAGGA-QVEFADI 61

Query: 73  GCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNSMK 132
           GCG+GGLL+ LS LFP+ L++G+E+R KVS+YV++RI +LRVA PG+YQNI+ +R+N+MK
Sbjct: 62  GCGYGGLLVQLSLLFPQQLILGLEIRVKVSDYVQDRIRSLRVAEPGRYQNIACLRSNAMK 121

Query: 133 YIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVEEL 192
           Y+PN+F K QLSKMFFLFPDPHFK+  H+ R+ISP LL EYAY LR+GG++YT TDVEE+
Sbjct: 122 YLPNFFRKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRIGGLVYTNTDVEEV 181

Query: 193 GEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRV 249
            EW+     +HP F  +T E+L  D  V  L + TEEG+KV RN G+ F AV+RRV
Sbjct: 182 HEWIVQHFSDHPLFSRVTEEQLADDIIVGHLGTCTEEGKKVQRNGGKNFLAVFRRV 236

BLAST of CmaCh05G006110 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 397.1 bits (1019), Expect = 1.0e-110
Identity = 197/250 (78.80%), Postives = 221/250 (88.40%), Query Frame = 0

Query: 2   LDNDAN-PTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQ 61
           +DN+    TFSKSTGLPRKRFYRARAHSNPLSDSHFP+PISP  +DYSLH+P+   ++++
Sbjct: 1   MDNETKATTFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNK 60

Query: 62  RDRTKKIEFADIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILAL-RVANPGQ 121
               KK+EFADIGCGFGGLLISL+TLFP+ LMIGMELRDKV+EYVKERILAL R ++ GQ
Sbjct: 61  --FIKKVEFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQ 120

Query: 122 YQNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRV 181
           Y+NISVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS  LLDEYAY LR 
Sbjct: 121 YENISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRA 180

Query: 182 GGIIYTITDVEELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGR 241
           GGIIYTITDVEELGEWMK+CLE HP FE+LT EEL +DP VELL SATEEGQKVARN G+
Sbjct: 181 GGIIYTITDVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQ 240

Query: 242 TFQAVYRRVA 250
           TF+AV+RR+A
Sbjct: 241 TFRAVFRRIA 248

BLAST of CmaCh05G006110 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 48.5 bits (114), Expect = 9.0e-06
Identity = 56/234 (23.93%), Postives = 101/234 (43.16%), Query Frame = 0

Query: 23  RARAHSNPLSDSH---FPVPISPNEIDYSLHYPQLFPSNDQRDRTKKIEFADIGCGFGGL 82
           R R H NPLS S     PVP+  +E+      P +                DIG G G  
Sbjct: 95  RIRQHVNPLSSSFSKPAPVPVW-DEVYKDPSLPLM---------------VDIGSGSGRF 154

Query: 83  LISLSTLFPEAL-MIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNSMKYIPNYF 142
           L+  +    E+   +G+E+R K+ +     +  L ++      N+  +  N+M    +  
Sbjct: 155 LLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLS------NVHFIFANAMVSFEHLI 214

Query: 143 EK--AQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVEELGEWM 202
                 L  +  L PDPHFK+++ +RRV+   L++     L+ GG I+  +DV ++ + M
Sbjct: 215 SSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLKPGGKIFVQSDVLDVAQDM 274

Query: 203 KACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQA--VYRRV 249
           +  L+     E+  L+ ++     +   +    G +  R     F+   +YRR+
Sbjct: 275 RDQLDE----ESNVLQHMDTVDTEDGWLTENPMGIRTEREIHAEFEGARIYRRL 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXB71.5e-10978.80tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24... [more]
A2YB342.8e-10576.99tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN... [more]
Q67V782.8e-10576.99tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 ... [more]
B6SHG75.0e-10275.31tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1[more]
A9UMM12.3e-7556.36tRNA (guanine-N(7)-)-methyltransferase B OS=Xenopus tropicalis OX=8364 GN=mettl1... [more]
Match NameE-valueIdentityDescription
AT5G24840.11.0e-11078.80tRNA (guanine-N-7) methyltransferase [more]
AT5G17660.19.0e-0623.93tRNA (guanine-N-7) methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 67..242
e-value: 3.3E-50
score: 169.9
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typeTIGRFAMTIGR00091TIGR00091coord: 64..247
e-value: 9.2E-41
score: 137.3
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePANTHERPTHR234173-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASEcoord: 11..249
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 19..247
score: 58.310261
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 45..247
e-value: 1.4E-80
score: 271.8
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 69..186
e-value: 1.95518E-4
score: 37.7947
IPR025763tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryotePANTHERPTHR23417:SF16TRNA (GUANINE-N(7)-)-METHYLTRANSFERASEcoord: 11..249
IPR025763tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryoteHAMAPMF_03055tRNA_methyltr_TrmB_eukcoord: 16..248
score: 44.102768
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 42..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G006110.1CmaCh05G006110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005634 nucleus
cellular_component GO:0043527 tRNA methyltransferase complex
molecular_function GO:0000049 tRNA binding
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity