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Sequences
The following sequences are available for this feature:
Gene sequence (with intron) Legend: exonCDSpolypeptide Hold the cursor over a type above to highlight its positions in the sequence below. ATGTTGGACAACGATGCTAATCCAACTTTCAGCAAGTCAACTGGCCTGCCACGAAAGCGCTTCTACCGTGCAAGAGCACACAGCAACCCTCTAAGTGACTCTCACTTCCCAGTACCAATCTCCCCCAATGAAATTGATTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGACCAGCGAGATAGAACCAAAAAGATTGAATTTGCTGATATTGGTTGCGGTTTTGGTGGTCTTCTGATTAGTCTTTCAACCCTTTTCCCGGAAGCCCTGATGATTGGAATGGAACTCCGGGATAAAGTATCGGAGTACGTTAAGGAACGGATCTTAGCTTTGAGAGTAGCAAATCCTGGCCAATACCAAAACATTTCAGTAGTTCGAACAAATTCCATGAAGTACATTCCAAACTACTTCGAAAAAGCACAACTTTCAAAGATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCACCGCCGGCGTGTAATCAGTCCATTTTTGCTTGATGAGTATGCATATGCGCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGGCTTGTCTAGAGAATCACCCTTGGTTTGAAGCTTTAACATTGGAAGAGCTCGAGGCAGATCCAGCCGTGGAGCTTCTAAGTTCTGCAACGGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACGGACCTTTCAGGCAGTTTATAGGCGCGTTGCTGCAGCACCATGA mRNA sequence ATGTTGGACAACGATGCTAATCCAACTTTCAGCAAGTCAACTGGCCTGCCACGAAAGCGCTTCTACCGTGCAAGAGCACACAGCAACCCTCTAAGTGACTCTCACTTCCCAGTACCAATCTCCCCCAATGAAATTGATTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGACCAGCGAGATAGAACCAAAAAGATTGAATTTGCTGATATTGGTTGCGGTTTTGGTGGTCTTCTGATTAGTCTTTCAACCCTTTTCCCGGAAGCCCTGATGATTGGAATGGAACTCCGGGATAAAGTATCGGAGTACGTTAAGGAACGGATCTTAGCTTTGAGAGTAGCAAATCCTGGCCAATACCAAAACATTTCAGTAGTTCGAACAAATTCCATGAAGTACATTCCAAACTACTTCGAAAAAGCACAACTTTCAAAGATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCACCGCCGGCGTGTAATCAGTCCATTTTTGCTTGATGAGTATGCATATGCGCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGGCTTGTCTAGAGAATCACCCTTGGTTTGAAGCTTTAACATTGGAAGAGCTCGAGGCAGATCCAGCCGTGGAGCTTCTAAGTTCTGCAACGGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACGGACCTTTCAGGCAGTTTATAGGCGCGTTGCTGCAGCACCATGA Coding sequence (CDS) ATGTTGGACAACGATGCTAATCCAACTTTCAGCAAGTCAACTGGCCTGCCACGAAAGCGCTTCTACCGTGCAAGAGCACACAGCAACCCTCTAAGTGACTCTCACTTCCCAGTACCAATCTCCCCCAATGAAATTGATTATTCCCTTCATTACCCTCAACTGTTCCCTTCAAATGACCAGCGAGATAGAACCAAAAAGATTGAATTTGCTGATATTGGTTGCGGTTTTGGTGGTCTTCTGATTAGTCTTTCAACCCTTTTCCCGGAAGCCCTGATGATTGGAATGGAACTCCGGGATAAAGTATCGGAGTACGTTAAGGAACGGATCTTAGCTTTGAGAGTAGCAAATCCTGGCCAATACCAAAACATTTCAGTAGTTCGAACAAATTCCATGAAGTACATTCCAAACTACTTCGAAAAAGCACAACTTTCAAAGATGTTTTTCTTGTTCCCGGACCCTCATTTCAAAGAGAAGAATCACCGCCGGCGTGTAATCAGTCCATTTTTGCTTGATGAGTATGCATATGCGCTGAGAGTTGGTGGGATTATATACACAATTACAGATGTGGAAGAGCTTGGAGAGTGGATGAAGGCTTGTCTAGAGAATCACCCTTGGTTTGAAGCTTTAACATTGGAAGAGCTCGAGGCAGATCCAGCCGTGGAGCTTCTAAGTTCTGCAACGGAGGAAGGACAAAAGGTTGCAAGAAATGAGGGACGGACCTTTCAGGCAGTTTATAGGCGCGTTGCTGCAGCACCATGA Protein sequence MLDNDANPTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDRTKKIEFADIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVEELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVAAAP
Homology
BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: Q8GXB7 (tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24840 PE=2 SV=1) HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-109 Identity = 197/250 (78.80%), Postives = 221/250 (88.40%), Query Frame = 0 Query: 2 LDNDAN-PTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQ 61 +DN+ TFSKSTGLPRKRFYRARAHSNPLSDSHFP+PISP +DYSLH+P+ ++++ Sbjct: 1 MDNETKATTFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNK 60
Query: 62 RDRTKKIEFADIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILAL-RVANPGQ 121 KK+EFADIGCGFGGLLISL+TLFP+ LMIGMELRDKV+EYVKERILAL R ++ GQ Sbjct: 61 --FIKKVEFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQ 120
Query: 122 YQNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRV 181 Y+NISVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS LLDEYAY LR Sbjct: 121 YENISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRA 180
Query: 182 GGIIYTITDVEELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGR 241 GGIIYTITDVEELGEWMK+CLE HP FE+LT EEL +DP VELL SATEEGQKVARN G+ Sbjct: 181 GGIIYTITDVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQ 240
Query: 242 TFQAVYRRVA 250 TF+AV+RR+A Sbjct: 241 TFRAVFRRIA 248
BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: A2YB34 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_22311 PE=3 SV=1) HSP 1 Score: 382.9 bits (982), Expect = 2.8e-105 Identity = 184/239 (76.99%), Postives = 208/239 (87.03%), Query Frame = 0 Query: 16 LPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDR-----TKKIEFA 75 LPRKRFYRARAHSNPLSDSHFP+PISP+E+D S HYP+ FPS + R +I FA Sbjct: 18 LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77
Query: 76 DIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNS 135 D+GCGFGGLL+ LSTLFP+ LMIGMELRDKV+EYVKERILALR +NPG+Y NISVVRTNS Sbjct: 78 DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137
Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVE 195 MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS LLDEYAY + VGGIIYTITDVE Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197
Query: 196 ELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVA 250 ELGEWM++CLE HP FEA+ EE +ADP V+LLS+ATEEGQKVARN G+TFQA++RR++ Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256
BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: Q67V78 (tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0236900 PE=3 SV=1) HSP 1 Score: 382.9 bits (982), Expect = 2.8e-105 Identity = 184/239 (76.99%), Postives = 208/239 (87.03%), Query Frame = 0 Query: 16 LPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDR-----TKKIEFA 75 LPRKRFYRARAHSNPLSDSHFP+PISP+E+D S HYP+ FPS + R +I FA Sbjct: 18 LPRKRFYRARAHSNPLSDSHFPIPISPDEVDLSQHYPRYFPSGEGEARQGDAAVPRIRFA 77
Query: 76 DIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNS 135 D+GCGFGGLL+ LSTLFP+ LMIGMELRDKV+EYVKERILALR +NPG+Y NISVVRTNS Sbjct: 78 DVGCGFGGLLVGLSTLFPDTLMIGMELRDKVTEYVKERILALRASNPGKYDNISVVRTNS 137
Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVE 195 MKYIPNYF KAQLSKMFFLFPDPHFKEKNHRRRVIS LLDEYAY + VGGIIYTITDVE Sbjct: 138 MKYIPNYFRKAQLSKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 197
Query: 196 ELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVA 250 ELGEWM++CLE HP FEA+ EE +ADP V+LLS+ATEEGQKVARN G+TFQA++RR++ Sbjct: 198 ELGEWMRSCLEKHPLFEAIPEEETKADPVVKLLSTATEEGQKVARNGGQTFQAIFRRIS 256
BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: B6SHG7 (tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1) HSP 1 Score: 372.1 bits (954), Expect = 5.0e-102 Identity = 180/239 (75.31%), Postives = 204/239 (85.36%), Query Frame = 0 Query: 16 LPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRD-----RTKKIEFA 75 LPRKRFYRARAHSNPLSDSHFPVPISP E+D S HYP+ FP++ D ++I FA Sbjct: 13 LPRKRFYRARAHSNPLSDSHFPVPISPEEVDLSQHYPRYFPADKGSDGEEAAAPQQIRFA 72
Query: 76 DIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNS 135 D+GCGFGGLL+ LS LFP+ LMIGMELRDKV+EYVKERILALR ANPGQY NISVVRTNS Sbjct: 73 DVGCGFGGLLVGLSPLFPDTLMIGMELRDKVTEYVKERILALRGANPGQYDNISVVRTNS 132
Query: 136 MKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVE 195 MKYIPNYF KAQL+KMFFLFPDPHFKEKNHRRRVIS LLDEYAY + VGGIIYTITDVE Sbjct: 133 MKYIPNYFRKAQLTKMFFLFPDPHFKEKNHRRRVISMQLLDEYAYVMEVGGIIYTITDVE 192
Query: 196 ELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRVA 250 ELGEWM++CLE HP FE + EE++ADP +LLS+ATEE QKVARN G+TF A++RR++ Sbjct: 193 ELGEWMRSCLEKHPLFETVPEEEIKADPVFKLLSTATEESQKVARNGGQTFYAIFRRIS 251
BLAST of CmaCh05G006110 vs. ExPASy Swiss-Prot
Match: A9UMM1 (tRNA (guanine-N(7)-)-methyltransferase B OS=Xenopus tropicalis OX=8364 GN=mettl1-B PE=2 SV=1) HSP 1 Score: 283.5 bits (724), Expect = 2.3e-75 Identity = 133/236 (56.36%), Postives = 179/236 (75.85%), Query Frame = 0 Query: 13 STGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQRDRTKKIEFADI 72 S +P+KR+YR RAHSNP++D F P+ P ++D+S YPQ FP ++ ++EFADI Sbjct: 2 SVCMPQKRYYRQRAHSNPMADHTFQYPVCPEQMDWSPLYPQYFPQQEEAGGA-QVEFADI 61
Query: 73 GCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNSMK 132 GCG+GGLL+ LS LFP+ L++G+E+R KVS+YV++RI +LRVA PG+YQNI+ +R+N+MK Sbjct: 62 GCGYGGLLVQLSLLFPQQLILGLEIRVKVSDYVQDRIRSLRVAEPGRYQNIACLRSNAMK 121
Query: 133 YIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVEEL 192 Y+PN+F K QLSKMFFLFPDPHFK+ H+ R+ISP LL EYAY LR+GG++YT TDVEE+ Sbjct: 122 YLPNFFRKGQLSKMFFLFPDPHFKKTKHKWRIISPTLLAEYAYTLRIGGLVYTNTDVEEV 181
Query: 193 GEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQAVYRRV 249 EW+ +HP F +T E+L D V L + TEEG+KV RN G+ F AV+RRV Sbjct: 182 HEWIVQHFSDHPLFSRVTEEQLADDIIVGHLGTCTEEGKKVQRNGGKNFLAVFRRV 236
BLAST of CmaCh05G006110 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase ) HSP 1 Score: 397.1 bits (1019), Expect = 1.0e-110 Identity = 197/250 (78.80%), Postives = 221/250 (88.40%), Query Frame = 0 Query: 2 LDNDAN-PTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPISPNEIDYSLHYPQLFPSNDQ 61 +DN+ TFSKSTGLPRKRFYRARAHSNPLSDSHFP+PISP +DYSLH+P+ ++++ Sbjct: 1 MDNETKATTFSKSTGLPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNK 60
Query: 62 RDRTKKIEFADIGCGFGGLLISLSTLFPEALMIGMELRDKVSEYVKERILAL-RVANPGQ 121 KK+EFADIGCGFGGLLISL+TLFP+ LMIGMELRDKV+EYVKERILAL R ++ GQ Sbjct: 61 --FIKKVEFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQ 120
Query: 122 YQNISVVRTNSMKYIPNYFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRV 181 Y+NISVVRTNSMKYIPNYFEK QLSKMFFLFPDPHFKEKNHRRRVIS LLDEYAY LR Sbjct: 121 YENISVVRTNSMKYIPNYFEKGQLSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRA 180
Query: 182 GGIIYTITDVEELGEWMKACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGR 241 GGIIYTITDVEELGEWMK+CLE HP FE+LT EEL +DP VELL SATEEGQKVARN G+ Sbjct: 181 GGIIYTITDVEELGEWMKSCLEKHPMFESLTQEELVSDPVVELLCSATEEGQKVARNGGQ 240
Query: 242 TFQAVYRRVA 250 TF+AV+RR+A Sbjct: 241 TFRAVFRRIA 248
BLAST of CmaCh05G006110 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase ) HSP 1 Score: 48.5 bits (114), Expect = 9.0e-06 Identity = 56/234 (23.93%), Postives = 101/234 (43.16%), Query Frame = 0 Query: 23 RARAHSNPLSDSH---FPVPISPNEIDYSLHYPQLFPSNDQRDRTKKIEFADIGCGFGGL 82 R R H NPLS S PVP+ +E+ P + DIG G G Sbjct: 95 RIRQHVNPLSSSFSKPAPVPVW-DEVYKDPSLPLM---------------VDIGSGSGRF 154
Query: 83 LISLSTLFPEAL-MIGMELRDKVSEYVKERILALRVANPGQYQNISVVRTNSMKYIPNYF 142 L+ + E+ +G+E+R K+ + + L ++ N+ + N+M + Sbjct: 155 LLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLS------NVHFIFANAMVSFEHLI 214
Query: 143 EK--AQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAYALRVGGIIYTITDVEELGEWM 202 L + L PDPHFK+++ +RRV+ L++ L+ GG I+ +DV ++ + M Sbjct: 215 SSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLKPGGKIFVQSDVLDVAQDM 274
Query: 203 KACLENHPWFEALTLEELEADPAVELLSSATEEGQKVARNEGRTFQA--VYRRV 249 + L+ E+ L+ ++ + + G + R F+ +YRR+ Sbjct: 275 RDQLDE----ESNVLQHMDTVDTEDGWLTENPMGIRTEREIHAEFEGARIYRRL 302
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q8GXB7 | 1.5e-109 | 78.80 | tRNA (guanine-N(7)-)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=At5g24... | [more] |
A2YB34 | 2.8e-105 | 76.99 | tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN... | [more] |
Q67V78 | 2.8e-105 | 76.99 | tRNA (guanine-N(7)-)-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 ... | [more] |
B6SHG7 | 5.0e-102 | 75.31 | tRNA (guanine-N(7)-)-methyltransferase OS=Zea mays OX=4577 PE=2 SV=1 | [more] |
A9UMM1 | 2.3e-75 | 56.36 | tRNA (guanine-N(7)-)-methyltransferase B OS=Xenopus tropicalis OX=8364 GN=mettl1... | [more] |
Match Name | E-value | Identity | Description | |
AT5G24840.1 | 1.0e-110 | 78.80 | tRNA (guanine-N-7) methyltransferase | [more] |
AT5G17660.1 | 9.0e-06 | 23.93 | tRNA (guanine-N-7) methyltransferase | [more] |
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR Term | IPR Description | Source | Source Term | Source Description | Alignment |
IPR003358 | tRNA (guanine-N-7) methyltransferase, Trmb type | PFAM | PF02390 | Methyltransf_4 | coord: 67..242 e-value: 3.3E-50 score: 169.9 |
IPR003358 | tRNA (guanine-N-7) methyltransferase, Trmb type | TIGRFAM | TIGR00091 | TIGR00091 | coord: 64..247 e-value: 9.2E-41 score: 137.3 |
IPR003358 | tRNA (guanine-N-7) methyltransferase, Trmb type | PANTHER | PTHR23417 | 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE | coord: 11..249 |
IPR003358 | tRNA (guanine-N-7) methyltransferase, Trmb type | PROSITE | PS51625 | SAM_MT_TRMB | coord: 19..247 score: 58.310261 |
None | No IPR available | GENE3D | 3.40.50.150 | Vaccinia Virus protein VP39 | coord: 45..247 e-value: 1.4E-80 score: 271.8 |
None | No IPR available | CDD | cd02440 | AdoMet_MTases | coord: 69..186 e-value: 1.95518E-4 score: 37.7947 |
IPR025763 | tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote | PANTHER | PTHR23417:SF16 | TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE | coord: 11..249 |
IPR025763 | tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote | HAMAP | MF_03055 | tRNA_methyltr_TrmB_euk | coord: 16..248 score: 44.102768 |
IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase | SUPERFAMILY | 53335 | S-adenosyl-L-methionine-dependent methyltransferases | coord: 42..214 |
Relationships
The following mRNA feature(s) are a part of this gene:
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category |
Term Accession |
Term Name |
biological_process |
GO:0106004 |
tRNA (guanine-N7)-methylation |
biological_process |
GO:0006400 |
tRNA modification |
cellular_component |
GO:0005634 |
nucleus |
cellular_component |
GO:0043527 |
tRNA methyltransferase complex |
molecular_function |
GO:0000049 |
tRNA binding |
molecular_function |
GO:0008176 |
tRNA (guanine-N7-)-methyltransferase activity |
|