CmaCh05G005930 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G005930
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncarotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like
LocationCma_Chr05: 2935895 .. 2939657 (-)
RNA-Seq ExpressionCmaCh05G005930
SyntenyCmaCh05G005930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCATAAGCAATTCTCTCAAGATGGCTTCCATGGCCGCCGGAAGCTGCATTAGCTTCAAAGCCTATAATCCATGTCTTCCCCTCCACCCTCCAAAACGCTCCGTTTCCAACTTCACCATCAGAAGTATAGCCAGTCCCATCCGCCCCTCGCCACCCGTCGTCGTTCCATTTCCTAATATCGACACCTCCACCCCCCCGACTCACGTTGCATGGACCAGCATTCGACAAGACCAATGGGAAGGACAACTCTCCATCCAAGGCCAATTGCCTCGTTGGCTGGTTCGTTTCTTAACCATTAAATTAAAATGGATACGATTCCGTGACATGCTTAAAATTAGCTTCAAACATGCTATGAAAATTTAAACATTCGATCGAAATCGATTTTAAAGCTTGCATGCATATGATTGAAAAAAATTCCATTCATATGGTGATTAATCATTATGGGAAAATAGTTTTTTTTTAATAATTTAAACAAATAAAAATTATTATTCTTAAAATATACTGATGGATATTCTTTGGTTTAATTGATTTATGTTTGTTGATGGGAACACAAATACTATAAAAAAAAAAAAAAATATGTTCATAACCTAAACAATTCTCTCAGTTGTCTTTTTCAAACAAGACATAACATCATGTGAATCATAAGATGTTGGTTAGTGTTTCATTCAAACTTTAATTACATTAATTAATGTTAATTATTGTACCTACATGCTCAATGAATATCATAACTATTTCAATATGATTGAAAAACTATATTTGGATGACATTTTTTATACTATAGTTTTTAATGTTTTATTAGATGATTGTCGTATTGATTAAATTGAATCATTTTTTTCATGTAGAATGGAACGTATTTGAGAAATGGACCTGGAGTATGGAATATTGGAAATTATAACTTTCGTCACCTTTTTGATGGTTATGCTACCATTGTTAAGCTTCAGTTTGAAGATGGTCGGTTAATTGCTGGACACCGTCAAATCGAATCGAATGCTTATAAGGCTGCCATGAAGAATCAAAAGGTTTGTTATCGTGAATTCTCTGAAGTTCCCAAGACTGATAACTTTCTTGCCTATGTTGGAGAGCTTGCAAACCTTTTCTCTGGTGTATCGTTGACTGATAATGCAAACACTGGAGTCGTGAAGCTTGGCGACAGTCGAGTCGTGTGTCTTACAGAGACGCAAAAGGGGTCTATAATAATCAACCCGAGTACGTTAGAAACCATGGGAAAGTTTGAGTATAGTGATTCACTTGGCGGTTTGATTCACTCGGCTCATCCAATTGTTACGGACTCGGAGTTTTTGACGTTGTTGCCAGATCTTTTAAACCCAGGTTACTTGGTGGTTCGGATGGAACCAGGCTCGAACGAGCGAAAGGTGATCGGTCGGGTCAACTGCAAGGGCGGACCAGCCCCAGGTTGGGTCCACTCGTTTCCAGTGACGGAGAATTATGTGGTGGTGCCGGAGATGCCATTGAGATATTGTGCTCAAAACTTGCTGAGAGCAGAGCCGACGCCGTTGTATAAGTTTGAGTGGCGGCCGGAGTCTAAAGCCTTTATGCATGTCATGTGCAAAGCTAGTGGAAATATTGTACGTCTTCCCTTTCTATCTTATTTTGCATTATTGCTGATACCTGTACACAAATTGCAAACAAGCTTATATGATATCAAATGACATTATTACTTATAATGCATACTTTTCAATGAAATGCAGGTTGCAAGCGTAGAAGTACCATTATTCATTACATTTCATTTCATAAATGCATATGAAGAGAGAGATGAAAAGGGAAGGATTACCGCTGTGATCGCCGATTGTTGCGAACATAATGCCAATCCTTTAATCTTAGATCGACTTAGGCTTCATAATTTACGACGCTTCTCGCTCAAACATGCTCTTCCAGATGCTAGGTTTGTTTGATCTTTTTATCATTCTTATCCAAACTGTTTGGAGTTTTTACTTTCATTCTTTAGTGTGTTTATTTGTAATGGCTCAAACCCGCCGCTAACAGATATTGTTGTTCTTTTTGGGTTTTTCCTTTCGGGCTTCCTCCCAAGGTTTTTCAAACGCGTCTGTTAGGGAGAGGTTTCCACACCCTTATAAAGAATACTTCGTTCCCCTCTTCAACCGATGTGGGATCTAACAATCCACCCCCTTTTTGGGTCCAGCGTCCTCGTTGGCACTCATTCCCTTCTCCAATCGATGAGGGACCTCCCCAATCCACTAACCCCCCTTTGGGGCTCAGCCTCCTCGCTGGCACATCGCCCGATATCTGGCTTTGGTACAATTTGTAACAGCTCAAGTTCACCGCTGACAAATATTGTCCTCTTCGGGTTTTCTCTTTCAGGCTTCTCCTTAAGGTTTTTAAAATGTTTTCGGATCTTATGTATGCATGCAGAGGGGGCATTATGAGCTGATGAACTGACCAAAGCAAAAAGATATATATATATATATATAGATATAACGAGGAAAGTTAATGGGATACAATGAATTGCAAAGCCGGAATTGTTGAATTTAAGAAAGAACAAAATAAACATACTCACAGCCGGAATGGTTGAATTCTTTTGAGAAAACTACTTACAATTCACCATTATCTGGTTTTGGCTTTGTCTTTGGCTTTGTCTTTTGCTTTTGCTTTGACTTGTTTGGATTTAGACTCCTCAAAGCATGCCATCATATAACACTTAAATTCACCAAGATTAGAAGAGATGGGATGAATAATTGGAAATGGAATTGTGTAGGGTTGGAAGGTTCAGAATACCATTGGATGGGAGTGGACATGGGAAGCTGGAGGCAGCATTGGACCCAGATGAACATGGCAGAGGAATGGACATGTGCAGCTTCAACCCTGCTTACTTAGGCAAGAAATATAGATATGCTTATGCTTGTGGAGCCAAAAGACCCTGCAATTTCCCCAACACACTCACTAAGGTAACACTTCCACAACCATTTTCCCTGACCTCTGCTTCCTCTTGTCTCTGGATCTTCCACCTCAATTCACTTTTGTGCTTGTTATGAATTTGTTTGTTTCAGATTGATTTGGTGAAGAAGAAGGCCAAGAATTGGTATGAAAGCGGTGCTATTCCTTCCGAGCCCTTCTTCGTGGCTCGACCTGGTGCAACCGAAGAGGACGACGGTAAGTCATTATATCATCTTCTAAGTGAATGAATTGAATAAACATACAACCTGAAAGTTCTCGGACTCGGAATCTTTTTCACCTTCTAACTAATGTAAGACGATTTTGGAGGTAAACTATGAAATCATATCATTAGTGAAAAAGGGAAGGAATGGAATGTGTCTAAAATGTATTTTGTGAATGCAGGAGTGGTGATCTCTATGGTCAGTGGGAAAAATGGAGAAGGGTATGCTTTGCTTCTAGATGGCTCCACATTTGAAGAGATAGCCAGGGCAAAATTTCCCTATGGTCTTCCTTATGGGCTACATGGTTGCTGGATTCCAAAGAATTAGTATCATTTCAATACTAAAGTGTAGCTTGTTCAATTAATCAGTATCAGAAAACACACATATATACATCATAGGCACGAGTAGAAAATACGTACATGTGGTGACTAACGATGCGCCGTAGCTCTACTGTGTATTAGCATATAGCTGGAGTAGATTATAGGTACCTTGTACAGAATTTAGGTCCCTTTTTCCTCATGTATTCATCAGTTTCTCTTCCACTTTGGACCTGTGAAATTAATATATACAGCTTTTCTTTGTACACTGGCCATGAATCCTTATATTGGATTCAACGGAGAGTAATTTG

mRNA sequence

AATCATAAGCAATTCTCTCAAGATGGCTTCCATGGCCGCCGGAAGCTGCATTAGCTTCAAAGCCTATAATCCATGTCTTCCCCTCCACCCTCCAAAACGCTCCGTTTCCAACTTCACCATCAGAAGTATAGCCAGTCCCATCCGCCCCTCGCCACCCGTCGTCGTTCCATTTCCTAATATCGACACCTCCACCCCCCCGACTCACGTTGCATGGACCAGCATTCGACAAGACCAATGGGAAGGACAACTCTCCATCCAAGGCCAATTGCCTCGTTGGCTGAATGGAACGTATTTGAGAAATGGACCTGGAGTATGGAATATTGGAAATTATAACTTTCGTCACCTTTTTGATGGTTATGCTACCATTGTTAAGCTTCAGTTTGAAGATGGTCGGTTAATTGCTGGACACCGTCAAATCGAATCGAATGCTTATAAGGCTGCCATGAAGAATCAAAAGGTTTGTTATCGTGAATTCTCTGAAGTTCCCAAGACTGATAACTTTCTTGCCTATGTTGGAGAGCTTGCAAACCTTTTCTCTGGTGTATCGTTGACTGATAATGCAAACACTGGAGTCGTGAAGCTTGGCGACAGTCGAGTCGTGTGTCTTACAGAGACGCAAAAGGGGTCTATAATAATCAACCCGAGTACGTTAGAAACCATGGGAAAGTTTGAGTATAGTGATTCACTTGGCGGTTTGATTCACTCGGCTCATCCAATTGTTACGGACTCGGAGTTTTTGACGTTGTTGCCAGATCTTTTAAACCCAGGTTACTTGGTGGTTCGGATGGAACCAGGCTCGAACGAGCGAAAGGTGATCGGTCGGGTCAACTGCAAGGGCGGACCAGCCCCAGGTTGGGTCCACTCGTTTCCAGTGACGGAGAATTATGTGGTGGTGCCGGAGATGCCATTGAGATATTGTGCTCAAAACTTGCTGAGAGCAGAGCCGACGCCGTTGTATAAGTTTGAGTGGCGGCCGGAGTCTAAAGCCTTTATGCATGTCATGTGCAAAGCTAGTGGAAATATTGTTGCAAGCGTAGAAGTACCATTATTCATTACATTTCATTTCATAAATGCATATGAAGAGAGAGATGAAAAGGGAAGGATTACCGCTGTGATCGCCGATTGTTGCGAACATAATGCCAATCCTTTAATCTTAGATCGACTTAGGCTTCATAATTTACGACGCTTCTCGCTCAAACATGCTCTTCCAGATGCTAGGGTTGGAAGGTTCAGAATACCATTGGATGGGAGTGGACATGGGAAGCTGGAGGCAGCATTGGACCCAGATGAACATGGCAGAGGAATGGACATGTGCAGCTTCAACCCTGCTTACTTAGGCAAGAAATATAGATATGCTTATGCTTGTGGAGCCAAAAGACCCTGCAATTTCCCCAACACACTCACTAAGATTGATTTGGTGAAGAAGAAGGCCAAGAATTGGTATGAAAGCGGTGCTATTCCTTCCGAGCCCTTCTTCGTGGCTCGACCTGGTGCAACCGAAGAGGACGACGGAGTGGTGATCTCTATGGTCAGTGGGAAAAATGGAGAAGGGTATGCTTTGCTTCTAGATGGCTCCACATTTGAAGAGATAGCCAGGGCAAAATTTCCCTATGGTCTTCCTTATGGGCTACATGGTTGCTGGATTCCAAAGAATTAGTATCATTTCAATACTAAAGTGTAGCTTGTTCAATTAATCAGTATCAGAAAACACACATATATACATCATAGGCACGAGTAGAAAATACGTACATGTGGTGACTAACGATGCGCCGTAGCTCTACTGTGTATTAGCATATAGCTGGAGTAGATTATAGGTACCTTGTACAGAATTTAGGTCCCTTTTTCCTCATGTATTCATCAGTTTCTCTTCCACTTTGGACCTGTGAAATTAATATATACAGCTTTTCTTTGTACACTGGCCATGAATCCTTATATTGGATTCAACGGAGAGTAATTTG

Coding sequence (CDS)

ATGGCTTCCATGGCCGCCGGAAGCTGCATTAGCTTCAAAGCCTATAATCCATGTCTTCCCCTCCACCCTCCAAAACGCTCCGTTTCCAACTTCACCATCAGAAGTATAGCCAGTCCCATCCGCCCCTCGCCACCCGTCGTCGTTCCATTTCCTAATATCGACACCTCCACCCCCCCGACTCACGTTGCATGGACCAGCATTCGACAAGACCAATGGGAAGGACAACTCTCCATCCAAGGCCAATTGCCTCGTTGGCTGAATGGAACGTATTTGAGAAATGGACCTGGAGTATGGAATATTGGAAATTATAACTTTCGTCACCTTTTTGATGGTTATGCTACCATTGTTAAGCTTCAGTTTGAAGATGGTCGGTTAATTGCTGGACACCGTCAAATCGAATCGAATGCTTATAAGGCTGCCATGAAGAATCAAAAGGTTTGTTATCGTGAATTCTCTGAAGTTCCCAAGACTGATAACTTTCTTGCCTATGTTGGAGAGCTTGCAAACCTTTTCTCTGGTGTATCGTTGACTGATAATGCAAACACTGGAGTCGTGAAGCTTGGCGACAGTCGAGTCGTGTGTCTTACAGAGACGCAAAAGGGGTCTATAATAATCAACCCGAGTACGTTAGAAACCATGGGAAAGTTTGAGTATAGTGATTCACTTGGCGGTTTGATTCACTCGGCTCATCCAATTGTTACGGACTCGGAGTTTTTGACGTTGTTGCCAGATCTTTTAAACCCAGGTTACTTGGTGGTTCGGATGGAACCAGGCTCGAACGAGCGAAAGGTGATCGGTCGGGTCAACTGCAAGGGCGGACCAGCCCCAGGTTGGGTCCACTCGTTTCCAGTGACGGAGAATTATGTGGTGGTGCCGGAGATGCCATTGAGATATTGTGCTCAAAACTTGCTGAGAGCAGAGCCGACGCCGTTGTATAAGTTTGAGTGGCGGCCGGAGTCTAAAGCCTTTATGCATGTCATGTGCAAAGCTAGTGGAAATATTGTTGCAAGCGTAGAAGTACCATTATTCATTACATTTCATTTCATAAATGCATATGAAGAGAGAGATGAAAAGGGAAGGATTACCGCTGTGATCGCCGATTGTTGCGAACATAATGCCAATCCTTTAATCTTAGATCGACTTAGGCTTCATAATTTACGACGCTTCTCGCTCAAACATGCTCTTCCAGATGCTAGGGTTGGAAGGTTCAGAATACCATTGGATGGGAGTGGACATGGGAAGCTGGAGGCAGCATTGGACCCAGATGAACATGGCAGAGGAATGGACATGTGCAGCTTCAACCCTGCTTACTTAGGCAAGAAATATAGATATGCTTATGCTTGTGGAGCCAAAAGACCCTGCAATTTCCCCAACACACTCACTAAGATTGATTTGGTGAAGAAGAAGGCCAAGAATTGGTATGAAAGCGGTGCTATTCCTTCCGAGCCCTTCTTCGTGGCTCGACCTGGTGCAACCGAAGAGGACGACGGAGTGGTGATCTCTATGGTCAGTGGGAAAAATGGAGAAGGGTATGCTTTGCTTCTAGATGGCTCCACATTTGAAGAGATAGCCAGGGCAAAATTTCCCTATGGTCTTCCTTATGGGCTACATGGTTGCTGGATTCCAAAGAATTAG

Protein sequence

MASMAAGSCISFKAYNPCLPLHPPKRSVSNFTIRSIASPIRPSPPVVVPFPNIDTSTPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSLTDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYESGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPKN
Homology
BLAST of CmaCh05G005930 vs. ExPASy Swiss-Prot
Match: Q8LIY8 (Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8B PE=1 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.4e-236
Identity = 378/534 (70.79%), Postives = 442/534 (82.77%), Query Frame = 0

Query: 22  HPPKRSVSNFTIRSIASPIRPSPPVVVPFPNIDTSTPPTH------------VAWTSIRQ 81
           H  KR+V+     S  +   P  PVV P P      P               VAW S+RQ
Sbjct: 32  HQGKRAVAQPLAASAVTEAAPPAPVVAP-PARPVDAPRRRGGRGGGGGGGELVAWKSVRQ 91

Query: 82  DQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGH 141
           ++WEG L + G+LP WL+GTYLRNGPG+WN+G+Y FRHLFDGYAT+V++ F  GR +  H
Sbjct: 92  ERWEGALEVDGELPLWLDGTYLRNGPGLWNLGDYGFRHLFDGYATLVRVSFRGGRAVGAH 151

Query: 142 RQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSLTDNANTGVVKLGD 201
           RQIES AYKAA  + KVCYREFSEVPK DNFL+YVG+LA LFSG SLTDN+NTGVV LGD
Sbjct: 152 RQIESEAYKAARAHGKVCYREFSEVPKPDNFLSYVGQLATLFSGSSLTDNSNTGVVMLGD 211

Query: 202 SRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLLNPG 261
            RV+CLTET KGSI ++P TL+T+GKF+Y+D LGGLIHSAHPIVTD+EF TL+PDL+ PG
Sbjct: 212 GRVLCLTETIKGSIQVDPDTLDTVGKFQYTDKLGGLIHSAHPIVTDTEFWTLIPDLIRPG 271

Query: 262 YLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPT 321
           Y+V RM+ GSNER+ +GRV+C+GGPAPGWVHSFPVTE+YVVVPEMPLRYCA+NLLRAEPT
Sbjct: 272 YVVARMDAGSNERQFVGRVDCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAKNLLRAEPT 331

Query: 322 PLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEERDEKGRITAVIADCC 381
           PLYKFEW  ES ++MHVMCKASG IVASVEVP F+TFHFINAYEE DE+GR+TA+IADCC
Sbjct: 332 PLYKFEWHLESGSYMHVMCKASGKIVASVEVPPFVTFHFINAYEETDEEGRVTAIIADCC 391

Query: 382 EHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLEAALDPDEHGRGMD 441
           EHNAN  ILD+LRLHNLR  S +  LPDARVGRFRIPLDGS  G+LE ALDP+EHGRGMD
Sbjct: 392 EHNANTAILDKLRLHNLRSSSGQDVLPDARVGRFRIPLDGSQFGELETALDPEEHGRGMD 451

Query: 442 MCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYESGAIPSEPFFVARP 501
           MCS NPA++G++YRYAYACGA+RPCNFPNTLTK+DLV++ AKNW+E G++PSEPFFV RP
Sbjct: 452 MCSINPAHVGREYRYAYACGARRPCNFPNTLTKVDLVERTAKNWHEEGSVPSEPFFVPRP 511

Query: 502 GATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPK 544
           GATEEDDGV ISMVS K+G GYAL+LDG TFEE+ARAKFPYGLPYGLH CW+P+
Sbjct: 512 GATEEDDGVAISMVSAKDGSGYALVLDGKTFEEVARAKFPYGLPYGLHCCWVPR 564

BLAST of CmaCh05G005930 vs. ExPASy Swiss-Prot
Match: Q8VY26 (Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD8 PE=1 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 8.3e-211
Identity = 342/518 (66.02%), Postives = 418/518 (80.69%), Query Frame = 0

Query: 34  RSIASPIRPSPPVVVPFPNIDTSTPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRN 93
           R++ +   PSP   +P           HVAWTS++Q+ WEG+L++QG++P WLNGTYLRN
Sbjct: 56  RAVINSAAPSP---LPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRN 115

Query: 94  GPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSE 153
           GPG+WNIG+++FRHLFDGY+T+VKLQF+ GR+ A HR +ES+AYKAA K+ ++CYREFSE
Sbjct: 116 GPGLWNIGDHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSE 175

Query: 154 VPKT----DNFLAYVGELANLFSGVSLTDNANTGVVKLGDSRVVCLTETQKGSIIINPST 213
            PK+     N  + +GE+  LFSG SLTDNANTGV+KLGD RV+CLTETQKGSI+++  T
Sbjct: 176 TPKSVIINKNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHET 235

Query: 214 LETMGKFEYSDSLGG-LIHSAHPIVTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRV 273
           LET+GKFEY D L   +I SAHPIVT++E  TL+PDL+ PGY VVRME GSN+R+V+GRV
Sbjct: 236 LETIGKFEYDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRV 295

Query: 274 NCKGGP-APGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVM 333
            C+ G   PGWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HVM
Sbjct: 296 RCRSGSWGPGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVM 355

Query: 334 CKASGNIVASVEVPLFITFHFINAYEE-RDEKGRITAVIADCCEHNANPLILDRLRLHNL 393
            K +G +VASVEVP ++TFHFINAYEE ++  G+ T +IADCCEHNA+  ILD LRL  L
Sbjct: 356 SKLTGEVVASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTL 415

Query: 394 RRFSLKHALPDARVGRFRIPLDGSGHGKLEAALDPDEHGRGMDMCSFNPAYLGKKYRYAY 453
           R       LPDAR+GRFRIPLDGS +GKLE A++ ++HGR MDMCS NP YLG+KYRY Y
Sbjct: 416 RSSHGHDVLPDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVY 475

Query: 454 ACGAKRPCNFPNTLTKIDLVKKKAKNWYESGAIPSEPFFVARPGATEEDDGVVISMVSGK 513
           ACGA+RPCNFPN L+K+D+V+KK KNW+E G IPSEPFFV RPGAT EDDGVVIS+VS +
Sbjct: 476 ACGAQRPCNFPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEE 535

Query: 514 NGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPKN 545
           NG  +A+LLDGS+FEEIARAKFPYGLPYGLHGCWIPK+
Sbjct: 536 NGGSFAILLDGSSFEEIARAKFPYGLPYGLHGCWIPKD 570

BLAST of CmaCh05G005930 vs. ExPASy Swiss-Prot
Match: Q93VD5 (Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8A PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 1.1e-194
Identity = 315/484 (65.08%), Postives = 384/484 (79.34%), Query Frame = 0

Query: 63  AWTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIVKLQFED 122
           A TS+RQ++WEG L I+G LP WLNGTY+RNGPG+W++G + F HLFDGYAT+V++ F  
Sbjct: 68  ASTSVRQERWEGDLPIEGCLPPWLNGTYIRNGPGMWDVGEHAFHHLFDGYATLVRVSFRG 127

Query: 123 G---RLIAGHRQIESNAYKAAMKNQKVCYREFSEVP-KTDNFLAYVGELANLFSGVSLTD 182
           G   R    HRQIES AY+AA+   +   REFS  P    + L   G+L  L +G +LTD
Sbjct: 128 GGGARATGAHRQIESEAYRAAVARGRPVLREFSHCPAPAKSLLHRFGDLVGLVTGAALTD 187

Query: 183 NANTGVVKLGDSRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTDSEF 242
           N N+ V+ LGD RV+CLTET K S++I+P TLET+G+F Y+D LGG++ SAHPIVTD+EF
Sbjct: 188 NPNSAVLPLGDGRVMCLTETTKSSVLIDPDTLETVGRFRYTDRLGGMVQSAHPIVTDTEF 247

Query: 243 LTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRY 302
           LTLLPDL+ PG+LVVRME GSNERKVIGR++C+GGP+PGW+HSF VTE Y VVPEMPLRY
Sbjct: 248 LTLLPDLVRPGHLVVRMEAGSNERKVIGRMDCRGGPSPGWLHSFAVTEKYAVVPEMPLRY 307

Query: 303 CAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEERDEK 362
            + +LL +E  P Y F+W P S ++MHVMCK++G  VASVEVP F+  HFINAYE   E+
Sbjct: 308 SSASLLASELAPFYAFDWVPASGSYMHVMCKSTGKTVASVEVPPFMAIHFINAYE---EE 367

Query: 363 GRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLEAA 422
           G   AV+ DCCEH  +P I++ L L  LR    K  LP+ARVGRFRIPLDGS  G+LE A
Sbjct: 368 GDEAAVVVDCCEHYGDPAIIETLVLSRLRLLRGKDVLPNARVGRFRIPLDGSPFGELETA 427

Query: 423 LDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYESGA 482
           LDP+EHGRGMDMCS NPA LG+KY+YAYACGA+RPCNFPNTLTKIDLV+KKAK+W+E G+
Sbjct: 428 LDPEEHGRGMDMCSINPARLGRKYQYAYACGARRPCNFPNTLTKIDLVEKKAKSWHEEGS 487

Query: 483 IPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHG 542
           +PSEPFFVARPGAT+EDDGVVIS+VS  +GEGYAL+LD +TFEEIAR +FPYGLPYG HG
Sbjct: 488 VPSEPFFVARPGATDEDDGVVISIVSSDDGEGYALVLDATTFEEIARVRFPYGLPYGFHG 547

BLAST of CmaCh05G005930 vs. ExPASy Swiss-Prot
Match: A8Y9I2 (Carotenoid isomerooxygenase OS=Galleria mellonella OX=7137 GN=ninaB PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.8e-48
Identity = 140/491 (28.51%), Postives = 229/491 (46.64%), Query Frame = 0

Query: 77  SIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGHRQIESNA 136
           +I G++P WL G+ LRNGPG   +G+  F HLFD  A + +    DG +    R ++SN 
Sbjct: 32  TITGEIPSWLQGSLLRNGPGSLKVGSMRFEHLFDSSALLHRFAINDGSVTYQCRFLQSNT 91

Query: 137 YKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFS-GVSLTDNANTGVVKLGDSRVVCL 196
           +K     +++   EF      D        +A LF  G SL+DNA   +   GD  +   
Sbjct: 92  FKKNRAAERIVVTEFGTRAVPDPCHTIFDRVAALFKPGESLSDNAMISLYPFGD-EIYAF 151

Query: 197 TETQKGSII--INPSTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLLNPGYL-- 256
           TE   G +I  I+P TL+T+ +    DS+  + H++HP V  +  +  L   +  G L  
Sbjct: 152 TE---GPVIHRIDPETLDTLERRNLMDSVSLVNHTSHPHVMPNGDVYNLGMSIVQGRLKH 211

Query: 257 VVRMEPGSNERKVIGRVNCKGGPAPGW------VHSFPVTENYVVVPEMPLRYCAQNLLR 316
           V+   P + +  +  + +     +P W      +H+F +TENY V+ E PL      +++
Sbjct: 212 VIVKFPYTEKGDMFAKAHIVANMSPRWPLHPAYMHTFGITENYFVIVEQPLSVSLLTMVK 271

Query: 317 AEPT--PL-YKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEERDEKGRIT 376
           ++P+  PL     W P  +  + ++ +  G  V           H INAYE         
Sbjct: 272 SQPSNEPLASSLHWYPNHETHIVLLSRRDGKEVKRYRTEPLFYLHIINAYEHDG------ 331

Query: 377 AVIADCCEHNANPLILDRLRLHNLRRFSLKHALPD---ARVGRFRIPLDGSGHGKLEAAL 436
            ++ D C +  +   +D + ++ +          +    R  R  +PL+ +   ++E  L
Sbjct: 332 VLVVDLCAYK-DAKAIDAMYINAIETMQSNADYAEWFRGRPKRLELPLNATNCSRIEPRL 391

Query: 437 DPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYESGAI 496
              + G       ++  Y G+ YRY YA  +      P T+ K+D    + K W ++   
Sbjct: 392 -LAQLGCETPRIHYD-LYNGRPYRYFYAISSDVDAANPGTIIKVDTKTGETKTWCDTNCY 451

Query: 497 PSEPFFVARPGATEEDDGVVISMV----SGKNGEGYALL-LDGSTFEEIARAKF--PYGL 544
           PSEP FV  PGATEEDDGV++S +    +G +    ALL L+     E+ARA F  P  +
Sbjct: 452 PSEPIFVPAPGATEEDDGVILSALVWGGAGAHCRRVALLVLEARGLRELARATFCAPSPV 509

BLAST of CmaCh05G005930 vs. ExPASy Swiss-Prot
Match: Q8HXG8 (Beta,beta-carotene 9',10'-oxygenase OS=Macaca fascicularis OX=9541 GN=BCO2 PE=2 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 9.0e-48
Identity = 146/521 (28.02%), Postives = 229/521 (43.95%), Query Frame = 0

Query: 78  IQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGHRQIESNAY 137
           + G  P+WLNG+ LR GPG +  G   + H FDG A + + +   G +    + ++S+ Y
Sbjct: 49  VWGHFPKWLNGSLLRIGPGKFEFGKDKYNHWFDGMALLHQFRMAKGTVTYRSKFLQSDTY 108

Query: 138 KAAMKNQKVCYREFSEV----PKTDNFLAYVGELANLFSGVSLTDNANTGVVKLGDSRVV 197
           KA     ++   EF  +    P  + F  ++          ++TDN N   V+      +
Sbjct: 109 KANSAKNRIVMSEFGTLATPDPCKNVFERFMSRFELPGKAAAMTDNTNVNYVRYKGDYYL 168

Query: 198 CLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLLNP---GY 257
           C TET   +  ++  TLE   K ++S  +     +AHP          + +   P    Y
Sbjct: 169 C-TETNFMN-KVDIETLEKTEKVDWSKFIAVNGATAHPHYDPDGTAYNMGNSFGPFGFSY 228

Query: 258 LVVRMEPGS---NERKVIGRVNCKGGPA----PGWVHSFPVTENYVVVPEMPLRYCAQNL 317
            V+R+ P      E     +V C   P     P + HSF +T NY++  E PL+    NL
Sbjct: 229 KVIRVPPEKVDLEETTHGAQVICSIAPTEKGKPSYYHSFGMTRNYIIFIEQPLK---MNL 288

Query: 318 LRAEPTPLY------KFEWRPESKAFMHVMCKASGNIV-ASVEVPLFITFHFINAYEERD 377
            +   + +          W P+     HV+ K +G ++        F+ FH INA+E++ 
Sbjct: 289 WKIATSKIRGKAFSDGISWEPQCNTRFHVVDKHTGQLLPGRYYSKPFVAFHHINAFEDQG 348

Query: 378 EKGRITAVIADCCEHNANPLILDRLRLHNLRRF-----SLKHALPDARVGRFRIPLDGSG 437
                  +I  CC+ N    IL+  +L NLR+       + ++   +   RF +PL+ S 
Sbjct: 349 -----CVIIDLCCQDNGR--ILEVYQLQNLRKAGEELDQVYNSAGRSFPRRFVLPLNVSL 408

Query: 438 HGKLEAALDP--------DEHGRGMDMCS--------------------FNPAYLGKKYR 497
           +      L P         +   G   CS                    +   + GKKYR
Sbjct: 409 NAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYGQFSGKKYR 468

Query: 498 YAYACGAKRPCNFPNTLTKIDLVKKKAKNWYESGAIPSEPFFVARPGATEEDDGVVISMV 543
           + Y CG +      ++L K+D+V K  K W E G  PSEP FV  PG  EED GV++S+V
Sbjct: 469 FFYGCGFRHLVG--DSLIKVDVVNKTLKVWREDGFYPSEPVFVPVPGTNEEDGGVILSVV 528

BLAST of CmaCh05G005930 vs. TAIR 10
Match: AT4G32810.1 (carotenoid cleavage dioxygenase 8 )

HSP 1 Score: 734.6 bits (1895), Expect = 5.9e-212
Identity = 342/518 (66.02%), Postives = 418/518 (80.69%), Query Frame = 0

Query: 34  RSIASPIRPSPPVVVPFPNIDTSTPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRN 93
           R++ +   PSP   +P           HVAWTS++Q+ WEG+L++QG++P WLNGTYLRN
Sbjct: 56  RAVINSAAPSP---LPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRN 115

Query: 94  GPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSE 153
           GPG+WNIG+++FRHLFDGY+T+VKLQF+ GR+ A HR +ES+AYKAA K+ ++CYREFSE
Sbjct: 116 GPGLWNIGDHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSE 175

Query: 154 VPKT----DNFLAYVGELANLFSGVSLTDNANTGVVKLGDSRVVCLTETQKGSIIINPST 213
            PK+     N  + +GE+  LFSG SLTDNANTGV+KLGD RV+CLTETQKGSI+++  T
Sbjct: 176 TPKSVIINKNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHET 235

Query: 214 LETMGKFEYSDSLGG-LIHSAHPIVTDSEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRV 273
           LET+GKFEY D L   +I SAHPIVT++E  TL+PDL+ PGY VVRME GSN+R+V+GRV
Sbjct: 236 LETIGKFEYDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRV 295

Query: 274 NCKGGP-APGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVM 333
            C+ G   PGWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HVM
Sbjct: 296 RCRSGSWGPGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVM 355

Query: 334 CKASGNIVASVEVPLFITFHFINAYEE-RDEKGRITAVIADCCEHNANPLILDRLRLHNL 393
            K +G +VASVEVP ++TFHFINAYEE ++  G+ T +IADCCEHNA+  ILD LRL  L
Sbjct: 356 SKLTGEVVASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTL 415

Query: 394 RRFSLKHALPDARVGRFRIPLDGSGHGKLEAALDPDEHGRGMDMCSFNPAYLGKKYRYAY 453
           R       LPDAR+GRFRIPLDGS +GKLE A++ ++HGR MDMCS NP YLG+KYRY Y
Sbjct: 416 RSSHGHDVLPDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVY 475

Query: 454 ACGAKRPCNFPNTLTKIDLVKKKAKNWYESGAIPSEPFFVARPGATEEDDGVVISMVSGK 513
           ACGA+RPCNFPN L+K+D+V+KK KNW+E G IPSEPFFV RPGAT EDDGVVIS+VS +
Sbjct: 476 ACGAQRPCNFPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEE 535

Query: 514 NGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPKN 545
           NG  +A+LLDGS+FEEIARAKFPYGLPYGLHGCWIPK+
Sbjct: 536 NGGSFAILLDGSSFEEIARAKFPYGLPYGLHGCWIPKD 570

BLAST of CmaCh05G005930 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 109.8 bits (273), Expect = 7.1e-24
Identity = 127/494 (25.71%), Postives = 214/494 (43.32%), Query Frame = 0

Query: 78  IQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIVKLQFEDGRLIAGHRQIESNAY 137
           I G LP  LNG Y+RNGP    +    + HLFDG   +  ++  +G+     R +++  Y
Sbjct: 130 IHGTLPLSLNGAYIRNGPNPQFLPRGPY-HLFDGDGMLHAIKIHNGKATLCSRYVKTYKY 189

Query: 138 KAAMKN----QKVCYREFSEVPKT--DNFLAYVGELANLFSGVSLTDNANTGVVKLGDSR 197
               +         +  F+ V  +     L     L   ++ V+    ANT +     +R
Sbjct: 190 NVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLA-FFSNR 249

Query: 198 VVCLTETQKGSII--INPSTLETMGKFEYSDSLGGLIHSAHPIVTD---SEFLTLLPDLL 257
           +  L E+     +       +ET+G++++   L  +  +AHP  TD    E        +
Sbjct: 250 LFALGESDLPYAVRLTESGDIETIGRYDFDGKL-AMSMTAHP-KTDPITGETFAFRYGPV 309

Query: 258 NPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL--RYCAQNLL 317
            P     R +    +++ +   +     +P ++H F +T+ + +  E+ L  R    +L+
Sbjct: 310 PPFLTYFRFDSAGKKQRDVPIFSM---TSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLV 369

Query: 318 RAEPTPLYKFEWRPESKAFMHVMCKASG--NIVASVEVPLFITFHFINAYEERDEKGRIT 377
               +P+     +      + V+ K +G  + +   EVP F   H INA++E D  G   
Sbjct: 370 LEGGSPVGTDNGKTPR---LGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDD--GNSV 429

Query: 378 AVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLEAALDPD 437
            +IA       N + ++    H L R  L HAL    V + +I L  +G  +        
Sbjct: 430 VLIA------PNIMSIE----HTLERMDLVHAL----VEKVKIDL-VTGIVRRHPI---- 489

Query: 438 EHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVK------KKAKNWYES 497
              R +D    NPA+LG+  RY YA     P    + + K+D+ K        A+  Y S
Sbjct: 490 -SARNLDFAVINPAFLGRCSRYVYA-AIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGS 549

Query: 498 GAIPSEPFFVAR-PG--ATEEDDGVVISMVSGK-NGEGYALLLDGST--FEEIARAKFPY 545
           G    EPFFVAR PG    EEDDG V++ V  +  GE   L++D  +   E +A  + P 
Sbjct: 550 GCYGGEPFFVARDPGNPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPR 590

BLAST of CmaCh05G005930 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 109.4 bits (272), Expect = 9.3e-24
Identity = 122/492 (24.80%), Postives = 208/492 (42.28%), Query Frame = 0

Query: 76  LSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATI--VKLQFEDG-----RLIAG 135
           L + GQ+P  L G Y+RNG            HLFDG   I  V + F++      R    
Sbjct: 117 LEVVGQIPSCLKGVYIRNGANPM-FPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKT 176

Query: 136 HRQIESNAYKAAMKNQKVC-YREFSEVPKTDNFLAYVGELANLFSGVSLTDNANTGVVKL 195
           +R ++  A   ++  + +      S + +   F A  G    L  G      AN GVV  
Sbjct: 177 NRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAG--IGLVDGTRGMGVANAGVV-F 236

Query: 196 GDSRVVCLTETQ--KGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIV--TDSEFLTLLP 255
            + R++ ++E        I     LET+G+F + D +   +  AHP V  T  +  TL  
Sbjct: 237 FNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSV-IAHPKVDATTGDLHTLSY 296

Query: 256 DLLNPGYL--VVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQ 315
           ++L   +L  +     G   R V   +     P P  +H F +TEN+VV+P+  + +   
Sbjct: 297 NVLKKPHLRYLKFNTCGKKTRDVEITL-----PEPTMIHDFAITENFVVIPDQQMVFKLS 356

Query: 316 NLLRAEPTPLYKFEWRPESKAFMHVMCK--ASGNIVASVEVPLFITFHFINAYEERDEKG 375
            ++R     +Y      E  A   V+ K   +G+ +  V+VP    FH  NA+EER E+G
Sbjct: 357 EMIRGGSPVIYV----KEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEG 416

Query: 376 RITAVIADCCEHNANPLILD-----RLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGK 435
               V+   C    + +  +     R+ L  + R +++    + +V    + L+ +GH  
Sbjct: 417 DPVIVVIGSCMSPPDTIFSESGEPTRVELSEI-RLNMRTKESNRKVIVTGVNLE-AGH-- 476

Query: 436 LEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNW- 495
                              N +Y+G+K ++ Y   A  P    + + K+D+       + 
Sbjct: 477 ------------------INRSYVGRKSQFVYIAIAD-PWPKCSGIAKVDIQNGTVSEFN 536

Query: 496 YESGAIPSEPFFVARPGATEEDDGVVISMV-SGKNGEGYALLLDGSTFEEIARAKFPYGL 545
           Y       EP FV   G  EED G V+  V   +  E   +++D +  +++A  + P  +
Sbjct: 537 YGPSRFGGEPCFVPE-GEGEEDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERV 570

BLAST of CmaCh05G005930 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 100.1 bits (248), Expect = 5.6e-21
Identity = 117/507 (23.08%), Postives = 204/507 (40.24%), Query Frame = 0

Query: 49  PFPNIDTSTPPTHVA--WTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFR 108
           P P   T+ P   +A  +  + +      L + G+LP  + G Y+RNG    +       
Sbjct: 112 PLPK--TADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLH-EPVTGH 171

Query: 109 HLFDGYATIVKLQFEDGRLIAGHRQIESNAY-KAAMKNQKVCYREFSEVPKTDN----FL 168
           H FDG   +  ++FE G      R  ++N + +     + V  +   E+          L
Sbjct: 172 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 231

Query: 169 AYVGELANLFSGVSLTDNANTGVVKLGDSRVVCLTETQKGSIIINPS-TLETMGKFEYSD 228
            Y    A +      T  AN G+V      +    +     + I P+  L+T+G+F++  
Sbjct: 232 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDG 291

Query: 229 SLGGLIHSAHPIV--TDSEFLTLLPDLLNPGYL-VVRMEP-GSNERKVIGRVNCKGGPAP 288
            L   +  AHP V     E   L  D+++  YL   R  P G+    V  +++      P
Sbjct: 292 QLESTM-IAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLD-----QP 351

Query: 289 GWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVA 348
             +H F +TEN+VVVP+  + +    ++R   +P+   + +      +    + S NI  
Sbjct: 352 TMMHDFAITENFVVVPDQQVVFKLPEMIRG-GSPVVYDKNKVARFGILDKYAEDSSNI-K 411

Query: 349 SVEVPLFITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALP 408
            ++ P    FH  NA+EE +       VI  C     +        + N    +LK  L 
Sbjct: 412 WIDAPDCFCFHLWNAWEEPETDE--VVVIGSCMTPPDS--------IFNESDENLKSVLS 471

Query: 409 DARVGRFRIPLDGSGHGKLEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNF 468
           + R+         +G       +  ++    ++    N   LG+K ++AY   A+ P   
Sbjct: 472 EIRLNL------KTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAE-PWPK 531

Query: 469 PNTLTKIDLVKKKA-KNWYESGAIPSEPFFVARPGATEEDDGVVISMV-SGKNGEGYALL 528
            +   K+DL   +  K+ Y       EP F+   G  EED+G ++  V   K  +    +
Sbjct: 532 VSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG-EEDEGYILCFVHDEKTWKSELQI 589

Query: 529 LDGSTFEEIARAKFPYGLPYGLHGCWI 542
           ++  + E  A  K P  +PYG HG +I
Sbjct: 592 VNAVSLEVEATVKLPSRVPYGFHGTFI 589

BLAST of CmaCh05G005930 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 89.7 bits (221), Expect = 7.6e-18
Identity = 119/520 (22.88%), Postives = 202/520 (38.85%), Query Frame = 0

Query: 51  PNIDTSTPPTHVA--WTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHL 110
           P+  T+ P   +A  +  + +      L + G +P  + G Y+RNG    +    +  HL
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLH-KPVSGHHL 231

Query: 111 FDGYATIVKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFL-AYVG-- 170
           FDG   +  ++F++G +    R  E+N     +  ++ C R     PK    L  ++G  
Sbjct: 232 FDGDGMVHAVRFDNGSVSYACRFTETN----RLVQERECGRPV--FPKAIGELHGHLGIA 291

Query: 171 -----ELANLFSGVSLTDN---ANTGVVKLGDSRVVCLTETQKGSI-IINPSTLETMGKF 230
                    LF  V  T     AN G+V      +    +     + +     LET G++
Sbjct: 292 KLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRY 351

Query: 231 EYSDSLGGLIHSAHPIVTDS--EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGP 290
           ++   L   +  AHP +     E   L  D+++  YL         E+     +      
Sbjct: 352 DFDGQLKSTM-IAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPL---D 411

Query: 291 APGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNI 350
            P  +H F +TEN+VV+P+  + +    ++R   +P+   E +      ++   K + +I
Sbjct: 412 QPTMIHDFAITENFVVIPDQQVVFRLPEMIRG-GSPVVYDEKKKSRFGILNKNAKDASSI 471

Query: 351 VASVEVPLFITFHFINAYEERDEKGRITAVIADCC--------EHNAN-PLILDRLRLHN 410
              +EVP    FH  N++EE +       VI  C         EH+     +L  +RL+ 
Sbjct: 472 -QWIEVPDCFCFHLWNSWEEPETDE--VVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNL 531

Query: 411 LRRFSLKHALPDARVGRFRIPLDGSGHGKLEAALDPDEHGRGMDMCSFNPAYLGKKYRYA 470
               S +  +   +V              LEA +              N   LG+K RYA
Sbjct: 532 KTGESTRRPVISEQV-------------NLEAGM-------------VNRNLLGRKTRYA 591

Query: 471 YACGAKRPCNFPNTLTKIDLVKKKAKNW-YESGAIPSEPFFVARPGATEEDDGVVISMVS 530
           Y      P    +   K+DL   + + + Y  G    EP F+   G  EED G ++  V 
Sbjct: 592 Y-LALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVH 648

Query: 531 GKNGEGYAL-LLDGSTFEEIARAKFPYGLPYGLHGCWIPK 544
            +      L L++    +  A    P  +PYG HG +I K
Sbjct: 652 DEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISK 648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LIY83.4e-23670.79Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp... [more]
Q8VY268.3e-21166.02Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q93VD51.1e-19465.08Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp... [more]
A8Y9I21.8e-4828.51Carotenoid isomerooxygenase OS=Galleria mellonella OX=7137 GN=ninaB PE=1 SV=1[more]
Q8HXG89.0e-4828.02Beta,beta-carotene 9',10'-oxygenase OS=Macaca fascicularis OX=9541 GN=BCO2 PE=2 ... [more]
Match NameE-valueIdentityDescription
AT4G32810.15.9e-21266.02carotenoid cleavage dioxygenase 8 [more]
AT4G19170.17.1e-2425.71nine-cis-epoxycarotenoid dioxygenase 4 [more]
AT3G24220.19.3e-2424.80nine-cis-epoxycarotenoid dioxygenase 6 [more]
AT3G14440.15.6e-2123.08nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G78390.17.6e-1822.88nine-cis-epoxycarotenoid dioxygenase 9 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 75..542
e-value: 2.0E-96
score: 324.0
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 61..544
NoneNo IPR availablePANTHERPTHR10543:SF118CAROTENOID CLEAVAGE DIOXYGENASE-RELATEDcoord: 61..544

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G005930.1CmaCh05G005930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen