CmaCh04G026670 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G026670
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionGlycerol-3-phosphate dehydrogenase [NAD(+)]
LocationCma_Chr04: 18107627 .. 18111177 (+)
RNA-Seq ExpressionCmaCh04G026670
SyntenyCmaCh04G026670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGACCTTAGTTTGGATCGCCAGATTTAACAAAATGCACGGATGCGCTTTGTTTCTCACGAAACGTGTATGGCTTCTCCGCTCTCTTAATTTCTCTTTATATTCTATTTCTTCTTCCTCTTCCCTTCTACTTCTCTCCACCGCTCGCTCCCATTTTCTTCCTTTTCTCCATTCTCTCCCTCCCATTTCTTCCATGGCTCCCGGGATCGAAGCTCCACAGCGTTCTAGCAATGGGGGCTCTGTTCGTCCCGATTCCCATTTCTCCGATGATGGGGTTTCTCGAAAATCCAAAGTTACCATCGTTGGAAGTGGAAATTGGGGCAGTGTTGCGGCTAAACTCATCGCCTCTAATGCGGCTAGGCTGAGTTCCTTCCATGGTATTTTCTTCAACTTCTGAAACATGGGATTTGTTTGTTATGCTACTGGATTTCGATTTGCGCCATTTTCGTTGCATTTTGATATCCGTTTCTTGAGTTTATTGGATTTCAGATGAGGTTCGAATGTGGGTGTTTGAGGAAACTTTGCCTACTGGTGAGAAGTTAACTGATGTTATCAATCTCAACAATGTAATGTTCTATTGATTAATTACGACCCATTTCCGTGTTAGGAAATTTAGAGCGTTATATATTAGTGATTCTTGAATCTTAATTAACAGGAGAATGTTAAGTATCTCCCTGGTATAAAGCTTGGCAAGAATGTTATTGCAGATCCAGACCTTGAAAATGCAGGTATTCCATTCAAGTTCTTCTGATTTTTTTGTTGATCTCATTCAAATCTTTCTCGCTTAGCATAGTCTAATGGGTTGAATTATCAGTGAAGGATGCTAATATGTTGGTGTTTGTGACACCCCACCAATTTGTGGAGGGGATATGCAAGAGACTTGTGGGAAAGATAAGGAAAGAAGTGGAAGCTATTTCCCTCATTAAGGGGATGGAGATGAAGAAGGAAGGTCCATGCATGATTTCTTCCCTCATCTCCCAGGAATTGGGTATCAATTGTTGTGTTCTAATGGGCGCTAACATAGCAAACGAGGTTACTTTTACAGATTCAAAGCCTTGTATGACAATTTGTTATGTATGTTTATATGTAGTAAGATTATGAAAACTGTTCATGACTCTGCTTTCAGGTTGATAAACAAGTAATCTTTAGAAACTTTTGTCTTAATTTTGCTTGATTTTAAAGTTCAGTTATGTTTTATTTCGTTTTGTTTCAAGATTGTTGAACTGTTCATGTAGATTGCTGTAGAGAAGTTTAGTGAAGCAACGGTGGGATACAGGGAGAACATGAGAGAGATTGCTGAAAAATGGGTTCAACTTTTCAGTACTCCCTATTTTGTTGTTTCACCTGTGAGTACAGTTCATGATCTCATGTATATTATTTAAGCAATCTCTGTCAATCCCAATTGTGATGAGTTTGATTCTGTTCAAAACTGATATATCACTCACAAATTCTGTGTAAAACTTGGTTTAACTCCTACTTAAGTTTTTGAATTTTGTATTTTTTGTCTCTAAATTTTGCATCTAAACTACTTGTGAGGAGAGGAGGACGGAGTATTCCTTATAAGGGTGTGAAAATCTCTCCCTAGTAGACACGTTTTAAAAACCTTAAGGGAAACCCCGAAAGGAAAAGTTTAAAGAGGACAATATCTTCTAGCGGTGGGCTTGGGCTGTTACACTGTTTTAGTCGTTGTGATTGATTTTTTGCATTAATCATAGCTATTTCAAATTGACTGGTGAAAAGCTTTCTTATTACCTTTCTAATTGTGAAATATTGAAAGGTCCAAGACGTTGAAGGAGTTGAAATGTGTGGAACCCTTAAAAATGTTGTGGCATTAGCAGCAGGTTAGTCGTAAATCTTTATCAATTCGCTTCTTTTTTTCTGCTTTTAATGAAGAACAGTCTTTAGCCAATTTGACATCACAGGTTTTGTTGATGGTTTGGAGATGGGAAATAACACAAAGGTACAAAACTTAGTTCTAAACATTCAAAAGGTAGATTACATGTCTTCTTTATCTCAGACTATTTTCATTTTCATTCGGTAGGCTGCGATAATGAGAATCGGTCTGAGAGAGATGAGGATGATCTCCAAGCTTCTGTTTTCATCTGTTAAGGACAGTACTTTCTTTGAGAGTTGTGGAGTTGCTGATCTGATTACAACGTGCTGTAAGGATAAATTCTTCATTCTTTTGTGCTTCTTCCTTTACTTTCTTTCATTGTCTCAGTAGAACCAATTTATATTTGGCAGTGGGAGGAAGAAACAGGAAGGTGGCTGCAGCTTTTGCAAAGCATGGAGGGAAGAGGTTAATATTGCTACCCTGAAACATGGATTTTTTATATGGAGGCTACGTAAAATTGTTGCTGTATATGATTTTACCTTCTCCCATGTAGGTCTTTTGATGAACTTGAAGCAGAGATGCTGCAGGGCCAAAAATTGCAGGTTTCTATTTCTCAATGACATGATTAACTCGTAGTTTTCGTAAATGGATCATTACCCTTTTCTTCCTTTTAGTTTTGAATTCAACATGTGGTGGAGGGGATTTGAACCTCGGGTTGGATGAGGTTGAATAGTTAATGTCTTTCTGTGGAGCGAGAGAATTTGAATATCTAACATCTTAGTCAAGGATATAGAGAGAATTTGAATGATATAGAGTTGAACTAGTTGAGCTTCGTTCTTAATGATTTTCATTTAGATATCTGGGGGAAGCAAGAGGGTTGTTTCTTGCTTAAATATTCACCAATTCCCTGATAAACAAATATTAGATTGTGTTCATCCTAAAAACTAGTGATGCAGGCATGTATAAACATTCTAGTTACATTATCAAGTAAAGTTGTTGTAGTTTCTCCTAACAGCTCATTTTACATTTTGAAGGGAGTCTCCACAGCAAAGGAGATGTACGAGGTTTTAAGTCACCGTGGGTGGCTCGAGCTGTTTCCATTGTTTGCATCAGTCCACGAGATCTGTATCGGTCATCTTTCACCATCAGCCATCGTCGAATATAGCGAGCGCAGACCCAACTTCTCTGTTATTAATGGCCAAGCTCAGTAGTTTTAACAAGACTGGCTCTCGCCTATTTCAGATGTAGTGTGTTCATGAAGGTAGTTGCATCGTTTTTGTACATTGAAGAAATTTGAGCATTGGGTCAATATCTGACTGAACTTTGCCTGATCTTTAGAAGTAAAATGAATGGCAGGTTGGTTACAGCAGCATTTGAGAGGTTGAAATGTGTTTCCTGAGGCAGCTTGGATTCTAAAAAATTCTCCGATAATATTGATTAGTTTCCATTCAATATTGCATTGATACTGAGAAGGTATGGAGATTGGTAATGGAAACAAGAACAAACAGGACACCATGTCCTTTTCTCATTTCATGATAGTTAACTCAAACATTTCAGAAGGTAAACTACACATCAAAAGGACCATAAGTTAATACATAGAACATAAAAAAAGGATCTGTGAAATTCTTACAATCCCACTCCTGTTCCTTAGAAACAATTTTGCTATACCCAACAATTATCCGGTGTGTATTCTGTCAAGTCGTTCGACACTCTTTCT

mRNA sequence

GCAGACCTTAGTTTGGATCGCCAGATTTAACAAAATGCACGGATGCGCTTTGTTTCTCACGAAACGTGTATGGCTTCTCCGCTCTCTTAATTTCTCTTTATATTCTATTTCTTCTTCCTCTTCCCTTCTACTTCTCTCCACCGCTCGCTCCCATTTTCTTCCTTTTCTCCATTCTCTCCCTCCCATTTCTTCCATGGCTCCCGGGATCGAAGCTCCACAGCGTTCTAGCAATGGGGGCTCTGTTCGTCCCGATTCCCATTTCTCCGATGATGGGGTTTCTCGAAAATCCAAAGTTACCATCGTTGGAAGTGGAAATTGGGGCAGTGTTGCGGCTAAACTCATCGCCTCTAATGCGGCTAGGCTGAGTTCCTTCCATGATGAGGTTCGAATGTGGGTGTTTGAGGAAACTTTGCCTACTGGTGAGAAGTTAACTGATGTTATCAATCTCAACAATGAGAATGTTAAGTATCTCCCTGGTATAAAGCTTGGCAAGAATGTTATTGCAGATCCAGACCTTGAAAATGCAGTGAAGGATGCTAATATGTTGGTGTTTGTGACACCCCACCAATTTGTGGAGGGGATATGCAAGAGACTTGTGGGAAAGATAAGGAAAGAAGTGGAAGCTATTTCCCTCATTAAGGGGATGGAGATGAAGAAGGAAGGTCCATGCATGATTTCTTCCCTCATCTCCCAGGAATTGGGTATCAATTGTTGTGTTCTAATGGGCGCTAACATAGCAAACGAGATTGCTGTAGAGAAGTTTAGTGAAGCAACGGTGGGATACAGGGAGAACATGAGAGAGATTGCTGAAAAATGGGTTCAACTTTTCAGTACTCCCTATTTTGTTGTTTCACCTGTCCAAGACGTTGAAGGAGTTGAAATGTGTGGAACCCTTAAAAATGTTGTGGCATTAGCAGCAGGTTTTGTTGATGGTTTGGAGATGGGAAATAACACAAAGGCTGCGATAATGAGAATCGGTCTGAGAGAGATGAGGATGATCTCCAAGCTTCTGTTTTCATCTGTTAAGGACAGTACTTTCTTTGAGAGTTGTGGAGTTGCTGATCTGATTACAACGTGCTTGGGAGGAAGAAACAGGAAGGTGGCTGCAGCTTTTGCAAAGCATGGAGGGAAGAGGTCTTTTGATGAACTTGAAGCAGAGATGCTGCAGGGCCAAAAATTGCAGGGAGTCTCCACAGCAAAGGAGATGTACGAGGTTTTAAGTCACCGTGGGTGGCTCGAGCTGTTTCCATTGTTTGCATCAGTCCACGAGATCTGTATCGGTCATCTTTCACCATCAGCCATCGTCGAATATAGCGAGCGCAGACCCAACTTCTCTGTTATTAATGGCCAAGCTCAGTAGTTTTAACAAGACTGGCTCTCGCCTATTTCAGATGTAGTGTGTTCATGAAGGTTGGTTACAGCAGCATTTGAGAGGTTGAAATGTGTTTCCTGAGGCAGCTTGGATTCTAAAAAATTCTCCGATAATATTGATTAGTTTCCATTCAATATTGCATTGATACTGAGAAGGTATGGAGATTGGTAATGGAAACAAGAACAAACAGGACACCATGTCCTTTTCTCATTTCATGATAGTTAACTCAAACATTTCAGAAGGTAAACTACACATCAAAAGGACCATAAGTTAATACATAGAACATAAAAAAAGGATCTGTGAAATTCTTACAATCCCACTCCTGTTCCTTAGAAACAATTTTGCTATACCCAACAATTATCCGGTGTGTATTCTGTCAAGTCGTTCGACACTCTTTCT

Coding sequence (CDS)

ATGCACGGATGCGCTTTGTTTCTCACGAAACGTGTATGGCTTCTCCGCTCTCTTAATTTCTCTTTATATTCTATTTCTTCTTCCTCTTCCCTTCTACTTCTCTCCACCGCTCGCTCCCATTTTCTTCCTTTTCTCCATTCTCTCCCTCCCATTTCTTCCATGGCTCCCGGGATCGAAGCTCCACAGCGTTCTAGCAATGGGGGCTCTGTTCGTCCCGATTCCCATTTCTCCGATGATGGGGTTTCTCGAAAATCCAAAGTTACCATCGTTGGAAGTGGAAATTGGGGCAGTGTTGCGGCTAAACTCATCGCCTCTAATGCGGCTAGGCTGAGTTCCTTCCATGATGAGGTTCGAATGTGGGTGTTTGAGGAAACTTTGCCTACTGGTGAGAAGTTAACTGATGTTATCAATCTCAACAATGAGAATGTTAAGTATCTCCCTGGTATAAAGCTTGGCAAGAATGTTATTGCAGATCCAGACCTTGAAAATGCAGTGAAGGATGCTAATATGTTGGTGTTTGTGACACCCCACCAATTTGTGGAGGGGATATGCAAGAGACTTGTGGGAAAGATAAGGAAAGAAGTGGAAGCTATTTCCCTCATTAAGGGGATGGAGATGAAGAAGGAAGGTCCATGCATGATTTCTTCCCTCATCTCCCAGGAATTGGGTATCAATTGTTGTGTTCTAATGGGCGCTAACATAGCAAACGAGATTGCTGTAGAGAAGTTTAGTGAAGCAACGGTGGGATACAGGGAGAACATGAGAGAGATTGCTGAAAAATGGGTTCAACTTTTCAGTACTCCCTATTTTGTTGTTTCACCTGTCCAAGACGTTGAAGGAGTTGAAATGTGTGGAACCCTTAAAAATGTTGTGGCATTAGCAGCAGGTTTTGTTGATGGTTTGGAGATGGGAAATAACACAAAGGCTGCGATAATGAGAATCGGTCTGAGAGAGATGAGGATGATCTCCAAGCTTCTGTTTTCATCTGTTAAGGACAGTACTTTCTTTGAGAGTTGTGGAGTTGCTGATCTGATTACAACGTGCTTGGGAGGAAGAAACAGGAAGGTGGCTGCAGCTTTTGCAAAGCATGGAGGGAAGAGGTCTTTTGATGAACTTGAAGCAGAGATGCTGCAGGGCCAAAAATTGCAGGGAGTCTCCACAGCAAAGGAGATGTACGAGGTTTTAAGTCACCGTGGGTGGCTCGAGCTGTTTCCATTGTTTGCATCAGTCCACGAGATCTGTATCGGTCATCTTTCACCATCAGCCATCGTCGAATATAGCGAGCGCAGACCCAACTTCTCTGTTATTAATGGCCAAGCTCAGTAG

Protein sequence

MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEAPQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSPSAIVEYSERRPNFSVINGQAQ
Homology
BLAST of CmaCh04G026670 vs. ExPASy Swiss-Prot
Match: P52425 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cuphea lanceolata OX=3930 GN=GPDH PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 6.4e-169
Identity = 296/351 (84.33%), Postives = 327/351 (93.16%), Query Frame = 0

Query: 84  KSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENV 143
           +S+VT+VGSGNWGSVAAKLIA+N  +L SFHDEVRMWVFEETLP+GEKLTDVIN  NENV
Sbjct: 22  RSRVTVVGSGNWGSVAAKLIATNTLKLPSFHDEVRMWVFEETLPSGEKLTDVINQTNENV 81

Query: 144 KYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKG 203
           KYLPGIKLG+NV+ADPDLENAVKDANMLVFVTPHQF+EGICKRL GKI++  +A+SLIKG
Sbjct: 82  KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMEGICKRLEGKIQEGAQALSLIKG 141

Query: 204 MEMKKEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQ 263
           ME+K EGPCMISSLIS  LGINCCVLMGANIANEIAVEKFSEATVG+REN R+IAEKWVQ
Sbjct: 142 MEVKMEGPCMISSLISDLLGINCCVLMGANIANEIAVEKFSEATVGFREN-RDIAEKWVQ 201

Query: 264 LFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMIS 323
           LFSTPYF+VS V+DVEGVE+CGTLKN+VA+AAGFVDGLEMGNNTKAAIMRIGLREM+  S
Sbjct: 202 LFSTPYFMVSAVEDVEGVELCGTLKNIVAIAAGFVDGLEMGNNTKAAIMRIGLREMKAFS 261

Query: 324 KLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQ 383
           KLLF SVKD+TFFESCGVADLITTCLGGRNRKVA AFAK+GGKRSFD+LEAEML+GQKLQ
Sbjct: 262 KLLFPSVKDTTFFESCGVADLITTCLGGRNRKVAEAFAKNGGKRSFDDLEAEMLRGQKLQ 321

Query: 384 GVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSPSAIVEYSERRPNFS 435
           GVSTAKE+YEVL HRGWLELFPLF++VHEI  G L PSAIVEYSE++  FS
Sbjct: 322 GVSTAKEVYEVLGHRGWLELFPLFSTVHEISTGRLPPSAIVEYSEQKTIFS 371

BLAST of CmaCh04G026670 vs. ExPASy Swiss-Prot
Match: Q9SCX9 (Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DHAPRD PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.1e-167
Identity = 307/403 (76.18%), Postives = 343/403 (85.11%), Query Frame = 0

Query: 28  SSSLLLLSTARSHFLPFLHSLPPISSMAPGIEAPQRSSNGGSVRPDSHFSDDGVSRKSKV 87
           SSS+  LS +RS  L         ++M+P +E   R  NGG        +DD    KSKV
Sbjct: 9   SSSIFSLSHSRSPSLSSSRFSSLSAAMSPALE-KSRQGNGGC-------NDDS---KSKV 68

Query: 88  TIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLP 147
           T+VGSGNWGSVAAKLIASNA +L SFHDEVRMWVFEE LP GEKL DVIN  NENVKYLP
Sbjct: 69  TVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENVKYLP 128

Query: 148 GIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMK 207
           GIKLG+NV+ADPDLENAVKDANMLVFVTPHQF++GICK+L GKI  +VEAISL+KGME+K
Sbjct: 129 GIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKGMEVK 188

Query: 208 KEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLFST 267
           KEGPCMISSLIS++LGINCCVLMGANIANEIAVEKFSEATVGYR   REIA+ WVQLFST
Sbjct: 189 KEGPCMISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYR-GSREIADTWVQLFST 248

Query: 268 PYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLF 327
           PYF+V+PV DVEGVE+CGTLKNVVA+AAGFVDGLEMGNNTKAAIMRIGLREM+ +SKLLF
Sbjct: 249 PYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGLEMGNNTKAAIMRIGLREMKALSKLLF 308

Query: 328 SSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVST 387
            SVKDSTFFESCGVAD+ITTCLGGRNR+VA AFAK  GKRSFDELEAEMLQGQKLQGVST
Sbjct: 309 PSVKDSTFFESCGVADVITTCLGGRNRRVAEAFAKSRGKRSFDELEAEMLQGQKLQGVST 368

Query: 388 AKEMYEVLSHRGWLELFPLFASVHEICIGHLSPSAIVEYSERR 431
           A+E+YEVL H GWLE+FPLF++VH+IC G L P AIV+Y E +
Sbjct: 369 AREVYEVLKHCGWLEMFPLFSTVHQICTGRLQPEAIVQYRENK 399

BLAST of CmaCh04G026670 vs. ExPASy Swiss-Prot
Match: Q5EA88 (Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Bos taurus OX=9913 GN=GPD1 PE=2 SV=3)

HSP 1 Score: 341.3 bits (874), Expect = 1.7e-92
Identity = 177/330 (53.64%), Postives = 235/330 (71.21%), Query Frame = 0

Query: 86  KVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENVKY 145
           KV IVGSGNWGS  AK++  NAA+L+ F   V MWVFEE +  G KLT++IN  +ENVKY
Sbjct: 5   KVCIVGSGNWGSAIAKIVGGNAAQLAHFDPRVTMWVFEEDI-GGRKLTEIINTQHENVKY 64

Query: 146 LPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKGME 205
           LPG KL  NV+A PD+  A  DA++L+FV PHQF+  IC +L G ++ +   +SLIKG++
Sbjct: 65  LPGHKLPPNVVAVPDVVQAAADADILIFVVPHQFIGKICDQLKGHLKADTIGVSLIKGVD 124

Query: 206 MKKEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLF 265
              +G  +IS +I + LGI   VLMGANIANE+A EKF E T+G +       +   +L 
Sbjct: 125 EGPKGLKLISEVIGERLGIPMSVLMGANIANEVADEKFCETTIGSKNQAH--GQLLKELM 184

Query: 266 STPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKL 325
            TP F ++ VQ+V+ VE+CG LKN+VA+ AGF DGL  G+NTKAA++R+GL EM   +KL
Sbjct: 185 QTPNFRITVVQEVDTVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKL 244

Query: 326 LFS-SVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQG 385
             S SV  +TF ESCGVADLITTC GGRNRKVA AFA+ G  +S ++LE EML GQKLQG
Sbjct: 245 FCSGSVSSATFLESCGVADLITTCYGGRNRKVAEAFARTG--KSIEQLEKEMLNGQKLQG 304

Query: 386 VSTAKEMYEVLSHRGWLELFPLFASVHEIC 415
             TA+E++ +L H+G ++ FPLF +V+++C
Sbjct: 305 PQTARELHSILQHKGMVDKFPLFTAVYKVC 329

BLAST of CmaCh04G026670 vs. ExPASy Swiss-Prot
Match: P21695 (Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Homo sapiens OX=9606 GN=GPD1 PE=1 SV=4)

HSP 1 Score: 340.1 bits (871), Expect = 3.7e-92
Identity = 177/330 (53.64%), Postives = 236/330 (71.52%), Query Frame = 0

Query: 86  KVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENVKY 145
           KV IVGSGNWGS  AK++  NAA+L+ F   V MWVFEE +  G+KLT++IN  +ENVKY
Sbjct: 5   KVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDI-GGKKLTEIINTQHENVKY 64

Query: 146 LPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKGME 205
           LPG KL  NV+A PD+  A +DA++L+FV PHQF+  IC +L G ++     ISLIKG++
Sbjct: 65  LPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVD 124

Query: 206 MKKEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLF 265
               G  +IS +I + LGI   VLMGANIA+E+A EKF E T+G ++  +   +   +L 
Sbjct: 125 EGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQ--GQLLKELM 184

Query: 266 STPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKL 325
            TP F ++ VQ+V+ VE+CG LKNVVA+ AGF DGL  G+NTKAA++R+GL EM   +KL
Sbjct: 185 QTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKL 244

Query: 326 LFSS-VKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQG 385
             S  V  +TF ESCGVADLITTC GGRNRKVA AFA+ G  +S ++LE E+L GQKLQG
Sbjct: 245 FCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTG--KSIEQLEKELLNGQKLQG 304

Query: 386 VSTAKEMYEVLSHRGWLELFPLFASVHEIC 415
             TA+E+Y +L H+G ++ FPLF +V+++C
Sbjct: 305 PETARELYSILQHKGLVDKFPLFMAVYKVC 329

BLAST of CmaCh04G026670 vs. ExPASy Swiss-Prot
Match: Q8N335 (Glycerol-3-phosphate dehydrogenase 1-like protein OS=Homo sapiens OX=9606 GN=GPD1L PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.8e-92
Identity = 175/330 (53.03%), Postives = 235/330 (71.21%), Query Frame = 0

Query: 86  KVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENVKY 145
           KV IVGSGNWGS  AK+I +N  +L  F   V+MWVFEET+  G KLTD+IN ++ENVKY
Sbjct: 7   KVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETV-NGRKLTDIINNDHENVKY 66

Query: 146 LPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKGME 205
           LPG KL +NV+A  +L  AV+DA++LVFV PHQF+  IC  + G++ K+   I+LIKG++
Sbjct: 67  LPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGID 126

Query: 206 MKKEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLF 265
              EG  +IS +I +++GI+  VLMGANIANE+A EKF E T+G    + E    + +L 
Sbjct: 127 EGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIG--SKVMENGLLFKELL 186

Query: 266 STPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKL 325
            TP F ++ V D + VE+CG LKN+VA+ AGF DGL  G+NTKAA++R+GL EM   +++
Sbjct: 187 QTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARI 246

Query: 326 LF-SSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQG 385
                V  +TF ESCGVADLITTC GGRNR+VA AFA+ G  ++ +ELE EML GQKLQG
Sbjct: 247 FCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFARTG--KTIEELEKEMLNGQKLQG 306

Query: 386 VSTAKEMYEVLSHRGWLELFPLFASVHEIC 415
             T+ E+Y +L  +G L+ FPLF +V++IC
Sbjct: 307 PQTSAEVYRILKQKGLLDKFPLFTAVYQIC 331

BLAST of CmaCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1JS63 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111487070 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.0e-246
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEA 60
           MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEA
Sbjct: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEA 60

Query: 61  PQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMW 120
           PQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMW
Sbjct: 61  PQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMW 120

Query: 121 VFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFV 180
           VFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFV
Sbjct: 121 VFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFV 180

Query: 181 EGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAV 240
           EGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAV
Sbjct: 181 EGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAV 240

Query: 241 EKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDG 300
           EKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDG
Sbjct: 241 EKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDG 300

Query: 301 LEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAF 360
           LEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAF
Sbjct: 301 LEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAF 360

Query: 361 AKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSP 420
           AKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSP
Sbjct: 361 AKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSP 420

Query: 421 SAIVEYSERRPNFSVINGQAQ 442
           SAIVEYSERRPNFSVINGQAQ
Sbjct: 421 SAIVEYSERRPNFSVINGQAQ 441

BLAST of CmaCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1E2R9 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC111430266 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.5e-242
Identity = 437/442 (98.87%), Postives = 438/442 (99.10%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFL-PFLHSLPPISSMAPGIE 60
           MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTA SHFL  FLHSLPPISSMAPGIE
Sbjct: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTAPSHFLSSFLHSLPPISSMAPGIE 60

Query: 61  APQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120
           AP RSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM
Sbjct: 61  APPRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120

Query: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180
           WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF
Sbjct: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180

Query: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA 240
           VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA
Sbjct: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA 240

Query: 241 VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD 300
           VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD
Sbjct: 241 VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD 300

Query: 301 GLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAA 360
           GLEMGNNTKAAIMRIGLREMRMISKLLFSSV+DSTFFESCGVADLITTCLGGRNRKVAAA
Sbjct: 301 GLEMGNNTKAAIMRIGLREMRMISKLLFSSVRDSTFFESCGVADLITTCLGGRNRKVAAA 360

Query: 361 FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS 420
           FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS
Sbjct: 361 FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS 420

Query: 421 PSAIVEYSERRPNFSVINGQAQ 442
           PSAIVEYSERRPNFSVINGQAQ
Sbjct: 421 PSAIVEYSERRPNFSVINGQAQ 442

BLAST of CmaCh04G026670 vs. ExPASy TrEMBL
Match: A0A1S3C9D4 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo OX=3656 GN=LOC103498126 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.5e-224
Identity = 411/448 (91.74%), Postives = 422/448 (94.20%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLR------SLNFSLYSISSSSSLLLLSTARSHFL-PFLHSLPPISS 60
           MH CALFLTKRVW LR      S NFSLYSI SS SLL+LST  SHFL PFLHSL P SS
Sbjct: 1   MHRCALFLTKRVWQLRPFEPFQSPNFSLYSI-SSFSLLILSTPSSHFLSPFLHSLSPSSS 60

Query: 61  MAPGIEAPQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSF 120
           MAPGIEA QRSSNGGSVRPDSH SDDGVS K KVT+VGSGNWGSVAAKLIASNAARLS F
Sbjct: 61  MAPGIEALQRSSNGGSVRPDSHLSDDGVSSKFKVTVVGSGNWGSVAAKLIASNAARLSCF 120

Query: 121 HDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVF 180
           HDEVRMWV+EETLPTGEKLTDVIN NNENVKYLPGIKLGKNVIADPDLENAVKDANMLVF
Sbjct: 121 HDEVRMWVYEETLPTGEKLTDVINRNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVF 180

Query: 181 VTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGAN 240
           VTPHQFVEGICKRLVGKIR +VEAISLIKGME+KKEGPCMISSLISQELGINCCVLMGAN
Sbjct: 181 VTPHQFVEGICKRLVGKIRGDVEAISLIKGMEVKKEGPCMISSLISQELGINCCVLMGAN 240

Query: 241 IANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVAL 300
           IANEIAVEKFSEATVGYRENMREIAEKWVQLF+TPYFVV+PVQDVEGVEMCGTLKNVVAL
Sbjct: 241 IANEIAVEKFSEATVGYRENMREIAEKWVQLFTTPYFVVTPVQDVEGVEMCGTLKNVVAL 300

Query: 301 AAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRN 360
           AAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRN
Sbjct: 301 AAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRN 360

Query: 361 RKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEI 420
           RKVAAAFA +GGKRSFDELEAEMLQGQKLQGVSTAKEMYEVL+HRGWLELFPLFASVHEI
Sbjct: 361 RKVAAAFAMNGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHEI 420

Query: 421 CIGHLSPSAIVEYSERRPNFSVINGQAQ 442
           C GHLSPSAIVEYSER+PNFSVI+G AQ
Sbjct: 421 CTGHLSPSAIVEYSERKPNFSVIDGHAQ 447

BLAST of CmaCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1BW17 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LOC111006237 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.8e-212
Identity = 393/449 (87.53%), Postives = 409/449 (91.09%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLR--------SLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPIS 60
           MH CALFL KRVW LR        S +F LYS+ SSSS++ L T R   L FL   P  S
Sbjct: 2   MHRCALFLPKRVWQLRPYDYELFHSPHFPLYSV-SSSSIVFLPTLRRSLLHFLS--PSSS 61

Query: 61  SMAPGIEAPQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSS 120
           SMAPGIE+   S NG     DSHFSDD +SRKS+VT+VGSGNWGSVAAKLIASNA RLSS
Sbjct: 62  SMAPGIESRHPSLNG-----DSHFSDDEISRKSRVTVVGSGNWGSVAAKLIASNALRLSS 121

Query: 121 FHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 180
           FHDEVRMWV+EETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV
Sbjct: 122 FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 181

Query: 181 FVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGA 240
           FVTPHQFVEGICKRLVGKIR++VEAISLIKGME+KKEGPCMISSLISQELGINCCVLMGA
Sbjct: 182 FVTPHQFVEGICKRLVGKIRRDVEAISLIKGMEVKKEGPCMISSLISQELGINCCVLMGA 241

Query: 241 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVA 300
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVV+PVQDVEGVEMCGTLKNVVA
Sbjct: 242 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVTPVQDVEGVEMCGTLKNVVA 301

Query: 301 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 360
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV DSTFFESCGVADLITTCLGGR
Sbjct: 302 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVNDSTFFESCGVADLITTCLGGR 361

Query: 361 NRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE 420
           NRKVAAAFAK+GGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE
Sbjct: 362 NRKVAAAFAKNGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE 421

Query: 421 ICIGHLSPSAIVEYSERRPNFSVINGQAQ 442
           ICIGH SPSAIVEYSER+PNFSVI+G AQ
Sbjct: 422 ICIGHFSPSAIVEYSERKPNFSVIDGHAQ 442

BLAST of CmaCh04G026670 vs. ExPASy TrEMBL
Match: A0A6J1JYH7 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111489027 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 9.1e-211
Identity = 392/449 (87.31%), Postives = 406/449 (90.42%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLN--FSLYSISSSSSLLLLSTARSHFLP-----FLHSLPPISS 60
           MH CALFLTKRVW LR L   F   + S    LLLL  +   F P     FLHSL P SS
Sbjct: 52  MHRCALFLTKRVWQLRPLEEPFQSPNFSYILFLLLLLPSPLXFSPPLLPHFLHSLSPSSS 111

Query: 61  MAPGIEAPQRSSNGGSVR-PDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSS 120
           M  GIEA  RSSNGG V  PDSHFSDDGVSRKSKVT+VGS NWGSVAAKLIASNAARLSS
Sbjct: 112 MTLGIEARLRSSNGGCVMPPDSHFSDDGVSRKSKVTVVGSDNWGSVAAKLIASNAARLSS 171

Query: 121 FHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 180
           FHDEVRMWV+EETLPTGEKLTDVINLNNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 172 FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 231

Query: 181 FVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGA 240
           FVTPHQFVEGICKR+VGK+R++VEAISLIKGME+KK+GPCMISSLISQELGINCCVLMGA
Sbjct: 232 FVTPHQFVEGICKRMVGKMRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 291

Query: 241 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVA 300
           NIANEIAVEKFSEATVGYRENMRE AEKWVQLFST YFVV+PVQDVEGVEMCGTLKNVVA
Sbjct: 292 NIANEIAVEKFSEATVGYRENMRERAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 351

Query: 301 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 360
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV DSTFFESCGVADLITTCLGGR
Sbjct: 352 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVNDSTFFESCGVADLITTCLGGR 411

Query: 361 NRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE 420
           NRKVAAAFAK+ GKRSFDELEAEMLQGQKLQGVSTAKEMYEVL HRGWLELFPLFASVHE
Sbjct: 412 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLKHRGWLELFPLFASVHE 471

Query: 421 ICIGHLSPSAIVEYSERRPNFSVINGQAQ 442
           ICIGHLSPSAIVEYSER+PNF VI+G AQ
Sbjct: 472 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 500

BLAST of CmaCh04G026670 vs. NCBI nr
Match: XP_022990063.1 (glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita maxima])

HSP 1 Score: 862.1 bits (2226), Expect = 2.1e-246
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEA 60
           MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEA
Sbjct: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLPFLHSLPPISSMAPGIEA 60

Query: 61  PQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMW 120
           PQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMW
Sbjct: 61  PQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMW 120

Query: 121 VFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFV 180
           VFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFV
Sbjct: 121 VFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFV 180

Query: 181 EGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAV 240
           EGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAV
Sbjct: 181 EGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIAV 240

Query: 241 EKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDG 300
           EKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDG
Sbjct: 241 EKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDG 300

Query: 301 LEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAF 360
           LEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAF
Sbjct: 301 LEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAF 360

Query: 361 AKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSP 420
           AKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSP
Sbjct: 361 AKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSP 420

Query: 421 SAIVEYSERRPNFSVINGQAQ 442
           SAIVEYSERRPNFSVINGQAQ
Sbjct: 421 SAIVEYSERRPNFSVINGQAQ 441

BLAST of CmaCh04G026670 vs. NCBI nr
Match: XP_023536069.1 (glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.2e-243
Identity = 439/442 (99.32%), Postives = 439/442 (99.32%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFL-PFLHSLPPISSMAPGIE 60
           MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFL  FLHSLPPISSMAPGIE
Sbjct: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFLSSFLHSLPPISSMAPGIE 60

Query: 61  APQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120
           APQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM
Sbjct: 61  APQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120

Query: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180
           WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF
Sbjct: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180

Query: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA 240
           VEGICKRLVGKI KEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA
Sbjct: 181 VEGICKRLVGKIMKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA 240

Query: 241 VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD 300
           VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD
Sbjct: 241 VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD 300

Query: 301 GLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAA 360
           GLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAA
Sbjct: 301 GLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAA 360

Query: 361 FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS 420
           FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS
Sbjct: 361 FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS 420

Query: 421 PSAIVEYSERRPNFSVINGQAQ 442
           PSAIVEYSERRPNFSVINGQAQ
Sbjct: 421 PSAIVEYSERRPNFSVINGQAQ 442

BLAST of CmaCh04G026670 vs. NCBI nr
Match: XP_022922214.1 (glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita moschata])

HSP 1 Score: 848.2 bits (2190), Expect = 3.2e-242
Identity = 437/442 (98.87%), Postives = 438/442 (99.10%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFL-PFLHSLPPISSMAPGIE 60
           MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTA SHFL  FLHSLPPISSMAPGIE
Sbjct: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTAPSHFLSSFLHSLPPISSMAPGIE 60

Query: 61  APQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120
           AP RSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM
Sbjct: 61  APPRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120

Query: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180
           WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF
Sbjct: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180

Query: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA 240
           VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA
Sbjct: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEIA 240

Query: 241 VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD 300
           VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD
Sbjct: 241 VEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVD 300

Query: 301 GLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAA 360
           GLEMGNNTKAAIMRIGLREMRMISKLLFSSV+DSTFFESCGVADLITTCLGGRNRKVAAA
Sbjct: 301 GLEMGNNTKAAIMRIGLREMRMISKLLFSSVRDSTFFESCGVADLITTCLGGRNRKVAAA 360

Query: 361 FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS 420
           FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS
Sbjct: 361 FAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLS 420

Query: 421 PSAIVEYSERRPNFSVINGQAQ 442
           PSAIVEYSERRPNFSVINGQAQ
Sbjct: 421 PSAIVEYSERRPNFSVINGQAQ 442

BLAST of CmaCh04G026670 vs. NCBI nr
Match: KAG7033075.1 (Glycerol-3-phosphate dehydrogenase [NAD(+)], partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 838.6 bits (2165), Expect = 2.5e-239
Identity = 437/456 (95.83%), Postives = 438/456 (96.05%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTARSHFL-PFLHSLPPISSMAPGIE 60
           MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTA SHFL  FLHSLPPISSMAPGIE
Sbjct: 1   MHGCALFLTKRVWLLRSLNFSLYSISSSSSLLLLSTAPSHFLSSFLHSLPPISSMAPGIE 60

Query: 61  APQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120
           AP RSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM
Sbjct: 61  APPRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRM 120

Query: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180
           WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF
Sbjct: 121 WVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQF 180

Query: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANE-- 240
           VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANE  
Sbjct: 181 VEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGANIANEVT 240

Query: 241 ------------IAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCG 300
                       IAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCG
Sbjct: 241 FTDLKPCMTICYIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCG 300

Query: 301 TLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLI 360
           TLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV+DSTFFESCGVADLI
Sbjct: 301 TLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVRDSTFFESCGVADLI 360

Query: 361 TTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFP 420
           TTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFP
Sbjct: 361 TTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFP 420

Query: 421 LFASVHEICIGHLSPSAIVEYSERRPNFSVINGQAQ 442
           LFASVHEICIGHLSPSAIVEYSERRPNFSVINGQAQ
Sbjct: 421 LFASVHEICIGHLSPSAIVEYSERRPNFSVINGQAQ 456

BLAST of CmaCh04G026670 vs. NCBI nr
Match: XP_008458845.1 (PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis melo] >XP_008458846.1 PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis melo])

HSP 1 Score: 786.6 bits (2030), Expect = 1.1e-223
Identity = 411/448 (91.74%), Postives = 422/448 (94.20%), Query Frame = 0

Query: 1   MHGCALFLTKRVWLLR------SLNFSLYSISSSSSLLLLSTARSHFL-PFLHSLPPISS 60
           MH CALFLTKRVW LR      S NFSLYSI SS SLL+LST  SHFL PFLHSL P SS
Sbjct: 1   MHRCALFLTKRVWQLRPFEPFQSPNFSLYSI-SSFSLLILSTPSSHFLSPFLHSLSPSSS 60

Query: 61  MAPGIEAPQRSSNGGSVRPDSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSF 120
           MAPGIEA QRSSNGGSVRPDSH SDDGVS K KVT+VGSGNWGSVAAKLIASNAARLS F
Sbjct: 61  MAPGIEALQRSSNGGSVRPDSHLSDDGVSSKFKVTVVGSGNWGSVAAKLIASNAARLSCF 120

Query: 121 HDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVF 180
           HDEVRMWV+EETLPTGEKLTDVIN NNENVKYLPGIKLGKNVIADPDLENAVKDANMLVF
Sbjct: 121 HDEVRMWVYEETLPTGEKLTDVINRNNENVKYLPGIKLGKNVIADPDLENAVKDANMLVF 180

Query: 181 VTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGAN 240
           VTPHQFVEGICKRLVGKIR +VEAISLIKGME+KKEGPCMISSLISQELGINCCVLMGAN
Sbjct: 181 VTPHQFVEGICKRLVGKIRGDVEAISLIKGMEVKKEGPCMISSLISQELGINCCVLMGAN 240

Query: 241 IANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVAL 300
           IANEIAVEKFSEATVGYRENMREIAEKWVQLF+TPYFVV+PVQDVEGVEMCGTLKNVVAL
Sbjct: 241 IANEIAVEKFSEATVGYRENMREIAEKWVQLFTTPYFVVTPVQDVEGVEMCGTLKNVVAL 300

Query: 301 AAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRN 360
           AAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRN
Sbjct: 301 AAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRN 360

Query: 361 RKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEI 420
           RKVAAAFA +GGKRSFDELEAEMLQGQKLQGVSTAKEMYEVL+HRGWLELFPLFASVHEI
Sbjct: 361 RKVAAAFAMNGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHEI 420

Query: 421 CIGHLSPSAIVEYSERRPNFSVINGQAQ 442
           C GHLSPSAIVEYSER+PNFSVI+G AQ
Sbjct: 421 CTGHLSPSAIVEYSERKPNFSVIDGHAQ 447

BLAST of CmaCh04G026670 vs. TAIR 10
Match: AT5G40610.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 590.1 bits (1520), Expect = 1.5e-168
Identity = 307/403 (76.18%), Postives = 343/403 (85.11%), Query Frame = 0

Query: 28  SSSLLLLSTARSHFLPFLHSLPPISSMAPGIEAPQRSSNGGSVRPDSHFSDDGVSRKSKV 87
           SSS+  LS +RS  L         ++M+P +E   R  NGG        +DD    KSKV
Sbjct: 9   SSSIFSLSHSRSPSLSSSRFSSLSAAMSPALE-KSRQGNGGC-------NDDS---KSKV 68

Query: 88  TIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLP 147
           T+VGSGNWGSVAAKLIASNA +L SFHDEVRMWVFEE LP GEKL DVIN  NENVKYLP
Sbjct: 69  TVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENVKYLP 128

Query: 148 GIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMK 207
           GIKLG+NV+ADPDLENAVKDANMLVFVTPHQF++GICK+L GKI  +VEAISL+KGME+K
Sbjct: 129 GIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKGMEVK 188

Query: 208 KEGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLFST 267
           KEGPCMISSLIS++LGINCCVLMGANIANEIAVEKFSEATVGYR   REIA+ WVQLFST
Sbjct: 189 KEGPCMISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYR-GSREIADTWVQLFST 248

Query: 268 PYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLF 327
           PYF+V+PV DVEGVE+CGTLKNVVA+AAGFVDGLEMGNNTKAAIMRIGLREM+ +SKLLF
Sbjct: 249 PYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGLEMGNNTKAAIMRIGLREMKALSKLLF 308

Query: 328 SSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVST 387
            SVKDSTFFESCGVAD+ITTCLGGRNR+VA AFAK  GKRSFDELEAEMLQGQKLQGVST
Sbjct: 309 PSVKDSTFFESCGVADVITTCLGGRNRRVAEAFAKSRGKRSFDELEAEMLQGQKLQGVST 368

Query: 388 AKEMYEVLSHRGWLELFPLFASVHEICIGHLSPSAIVEYSERR 431
           A+E+YEVL H GWLE+FPLF++VH+IC G L P AIV+Y E +
Sbjct: 369 AREVYEVLKHCGWLEMFPLFSTVHQICTGRLQPEAIVQYRENK 399

BLAST of CmaCh04G026670 vs. TAIR 10
Match: AT2G40690.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 114.8 bits (286), Expect = 1.8e-25
Identity = 104/351 (29.63%), Postives = 170/351 (48.43%), Query Frame = 0

Query: 79  DGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVFEETLPTGEKLTDVINL 138
           D + R  KV ++G G++G+  A  +A     L     EV M V +        +   IN 
Sbjct: 82  DVLERTRKVVVLGGGSFGTAMAAHVARRKEGL-----EVNMLVRDSF------VCQSINE 141

Query: 139 NNENVKYLPGIKLGKNVIADPDLENAVKDANMLVFVTPHQFVEGICKRLVGKIRKEVEAI 198
           N+ N KY P  KL +NVIA  D + A+ DA+  +   P QF     + +   +   +  I
Sbjct: 142 NHHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFI 201

Query: 199 SLIKGMEMKKEGPCMISSLISQEL---GINCCVLMGANIANEIAVEKFSEATVGYRENMR 258
           SL KG+E+      M+S +I   L         L G + A E+     +   V  ++  +
Sbjct: 202 SLSKGLELNTLR--MMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKD--K 261

Query: 259 EIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIG 318
           ++A    QL ++ Y  ++   DV GVE+ G LKNV+A+AAG VDG+ +GNN+ AA++  G
Sbjct: 262 KLANAVQQLLASSYLRINTSSDVTGVEIAGALKNVLAIAAGIVDGMNLGNNSMAALVSQG 321

Query: 319 LREMRMISKLLFSSVKDSTFFESCGVADLITTCLG--GRNRKVAAAFAKHGGKRSFDELE 378
             E+R ++  +    K +T     G  D++ TC     RNR V     + G   + D++ 
Sbjct: 322 CSEIRWLATKM--GAKPTTITGLSGTGDIMLTCFVNLSRNRTVG---VRLGSGETLDDIL 381

Query: 379 AEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHEICIGHLSPSAIV 425
             M   Q  +GV+TA  +   L+ +  ++L P+  +V +I    L+P+  V
Sbjct: 382 TSM--NQVAEGVATAGAVI-ALAQKYNVKL-PVLTAVAKIIDNELTPTKAV 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524256.4e-16984.33Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cuphea lanceolata OX=3930 GN=GPDH... [more]
Q9SCX92.1e-16776.18Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic OS=Arabidopsis thal... [more]
Q5EA881.7e-9253.64Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Bos taurus OX=9913 G... [more]
P216953.7e-9253.64Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Homo sapiens OX=9606... [more]
Q8N3354.8e-9253.03Glycerol-3-phosphate dehydrogenase 1-like protein OS=Homo sapiens OX=9606 GN=GPD... [more]
Match NameE-valueIdentityDescription
A0A6J1JS631.0e-246100.00Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1E2R91.5e-24298.87Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A1S3C9D45.5e-22491.74Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A6J1BW174.8e-21287.53Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1JYH79.1e-21187.31Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_022990063.12.1e-246100.00glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita maxima][more]
XP_023536069.12.2e-24399.32glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita pepo subsp. pepo][more]
XP_022922214.13.2e-24298.87glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita moschata][more]
KAG7033075.12.5e-23995.83Glycerol-3-phosphate dehydrogenase [NAD(+)], partial [Cucurbita argyrosperma sub... [more]
XP_008458845.11.1e-22391.74PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis melo] >XP_008458... [more]
Match NameE-valueIdentityDescription
AT5G40610.11.5e-16876.18NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
AT2G40690.11.8e-2529.63NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPRINTSPR00077GPDHDRGNASEcoord: 270..294
score: 51.15
coord: 152..179
score: 47.19
coord: 295..319
score: 61.58
coord: 89..106
score: 55.72
coord: 228..248
score: 55.27
coord: 337..354
score: 75.25
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 82..432
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPROSITEPS00957NAD_G3PDHcoord: 285..306
IPR011128Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminalPFAMPF01210NAD_Gly3P_dh_Ncoord: 86..253
e-value: 2.0E-46
score: 157.8
IPR006109Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminalPFAMPF07479NAD_Gly3P_dh_Ccoord: 277..424
e-value: 6.9E-45
score: 152.8
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 278..432
e-value: 7.5E-57
score: 194.0
IPR017751Glycerol-3-phosphate dehydrogenase, NAD-dependent, eukaryoticTIGRFAMTIGR03376TIGR03376coord: 86..426
e-value: 3.9E-148
score: 491.1
NoneNo IPR availableGENE3D3.40.50.720coord: 71..275
e-value: 8.9E-74
score: 249.4
NoneNo IPR availablePIRSRPIRSR000114-1PIRSR000114-1coord: 84..429
e-value: 3.1E-99
score: 330.2
NoneNo IPR availablePIRSRPIRSR000114-2PIRSR000114-2coord: 84..429
e-value: 3.1E-99
score: 330.2
NoneNo IPR availablePIRSRPIRSR000114-3PIRSR000114-3coord: 84..429
e-value: 3.1E-99
score: 330.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..72
NoneNo IPR availablePANTHERPTHR11728:SF39GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]coord: 82..432
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 84..277
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 276..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G026670.1CmaCh04G026670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0046168 glycerol-3-phosphate catabolic process
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0009331 glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0004367 glycerol-3-phosphate dehydrogenase [NAD+] activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor