CmaCh04G022730 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G022730
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionIsocitrate dehydrogenase [NADP]
LocationCma_Chr04: 15872701 .. 15877431 (+)
RNA-Seq ExpressionCmaCh04G022730
SyntenyCmaCh04G022730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAAAAAAGGCGCCTGGTGCGGCGGCGGCTTTCATCCACCTACTGTATCTCTCCATTTTTTTTCTTGCTTCGATTTTTCCCTCTCTTTGCGCTCTCCTTATTCGATCTTCTCTGTGATTACTTCGTTTGAAGTGAGGGTGGTCAATAATCATGGCTTTTCAGAAGATCAAGGTCGCCAACCCCATCGTTGAGATGGACGGTGAGACCACTATGTCCCGATTTCAAGCTCATTTGAATGAGATATGTTCATGTTTGATCGTGTTTTTCGTTATTAATACTGTTGGATTGCTTTTGTAATTGCTAAATATCATCGGGAATGGGGATTTTTTACTTTCATATGTTACGAGGGAGTTCGTGTTTGGTTTTTTGGCCGTTAGATCTGTGAAGAACGGTGTGTGGTTTGTTAGATCATGGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATGTATGAGTAGTGATTCTTGCATCTTGAATTGATTTGTTGTGTTGTGCTCTGGCGGAAGTGAGTCAAGTGATTATGTTGTGGTTACACTTCTCTGATCTGAGACCAAAGCCTTAGCATTCACTTGTTACTGTTTGATGTATTTCTGTTTTGAGAAATGAGTATTTCTGTTTTCTTCTCTTTGTTGGTGGTCAGCTACCTCTGTTTTGAGGGAAGTTTTGGAATTTTGTCCCTCTGTTTGCCAGTCAGATGGGGGACAGTGTTTTTCTTTTCTTCCCCCTTCATTATCATAAGTTATTGTAAGCTGGAAACAAATCCAGTGGAATTAACGTCTGGATCTTTGTCGCCTTAAATAAATTTGTTGCACGATACACTTGGTCCACATAGTTCCATAGATTTACATTTTCAAGTGAAAAGCCTACTATTTTACTTATATTCTTGAATTATGCAGTCACATTTTAGTGAATAATTTAACGATTAGAACCAATGAAGTTCTGCCTACGATAGTTATTTTTATCTTTATTTACTTTATGTAATGCATGATTGGTGCAATACTTGTCTAGATGTGGAATAGTTAAGAGTATTTCCTTGAGTTTTCTTTAGATTTTCCTCTGTTCTGCATTGTTTCTTTTATTTTATTTTTTCTTTATTCGTAATGTTACTCTTGGAATCCTTTGTACAGGTGATGAAATGACTCGAGTTATTTGGGAATCAATTAAAAATAAGGTATTGCTCTCTCTTAATGTGTATGTGCATTTTAATTGTTAGTTATCTTGTTTGATTTTTAGCTGTCTTGTTTGTAGCTTATTTTCCCCTTCTTGGAATTGGACATCAAGTATTTTGACCTTGGCCTTCCACACCGTGATGCCACCGATGATAAAGTTACAATTGAAAGTGCAGAAGCTACTTTAAAGTATGTATTCCCAATGTTTATTTGGTGTTATTAGCGAGAATTATTTGAGTTATTTTGGTTGAATTATCTGATGGATCTAATTATACTTTATAATATCATGAGAAATTATAGTGGATTCTATTTATGTTATTTTTTAAGTAACGTATTCATTTTGGAATTCTGATTTTTCTAATCATGACCTCATTTTGTCTGGCTATACTCGTTTGTTCTAGGTACAATGTAGCAATCAAATGTGCAACAATTACTCCTGGTATAAATTGATTTACTTAATTGGAAAAGATCATCCAATTTAAGAGCTTATCTTGGGATTTTGTTGATTTTTACATTATTTTAAAGAGCACCTGGAGTCCTGATGTTCTAACAATGATGATTGACAGATGAAGCACGTGTTACGGAATTTGGCTTGAAGCAGATGTGGAAAAGTCCAAATGGGACGATCAGGAATATTCTGAATGGTCAGATCCCCCACCATATGTAATTTGTACTCCAGTTTGTATAAGTTTTTGAAAAAGCTTCCAAGGCTAATTACAAGTTTTAGAATATTTTGTTTTTCTAAAATCAAAGAAGATTTAGAAATATGTTATTGGTGTTTCTTTTGCACTCCACTGTTTTTTCTCCATATTTTTGAAATGGTTTGTGATTATTTGATTGTGAAGTGAGAATTTTGTCTCAGAATAGGTTTCCTTAGGGTCAACCTATTGCTGTCGTTACTTATTTTTATTTCAGACTCACCTGTTGGCATCTTCTGCAGGCACTGTCTTCAGAGAGCCAATTCTTTGCAAAAATGTTCCTCGTCTTGTACCAGGTAATTGCTGTCCATATTGAATTCATACTTAGGTTAGAAAATTGTTCTGATGATTGTTGTTTTCAGGTTGGACGAAGCCAATCTGTATTGGCAGACATGCTTTTGGTGATCAATACAAAGCTACTGATACAGTTATCAAAGGACCTGGAAAATTGAAATTGGTTTTTGGTATTTGAGATTCCCAAATCATCTCTCTAATGAATCTTAAATTTCTTTTCTTTGGAAGTTTCCTAACATGCTTACCCTTCCAGAGGGGCAAGAGACGCAAGAGATAGAGGTTTTCAACTTTACTGGTGCTGGAGGAGTAGCGTTGTCCATGTACAACACTGACGAGGTTTGTTATGATATCCTCATGGTTTAGTTAGGAACTTTAGAAAATATTATTGACCCATTTCAAGTTTCAACAAGTCTCTCAAATTAACCCTTCAATGTAATGCTGGTTTGTAGTCAATCCGCTCCTTCGCTGAGGCATCAATGAACACTGCTTATGAGAAGAAATGGCCTCTTTACCTTAGCACAAAAAATACTATTCTAAAGAAATATGATGGAAGGTATACACTTTGATTTCCTATTTTGTCCATGGACAATATGTAATTTTTACAGTAACTTTTTCTTTGCGTTCTCTGCATCAATAATTTCTTTCATTATGTTCATGTGTTGAATGAAGATTCAAGGATATATTCCAAGAAGTATACGAATCCCAGTGGAAATCCAAGTTCGAGGCTGCTGGCATATGGTGAATAGCTTTTAATGTTGCCTGGATAAATGTTTATTAAGCTATTCCAAGTTTGAACTGTTTAACTAATTATTCTGATTCTAAAAGGTATGAACACCGTCTCATTGATGATATGGTGGCTTATGCACTTAAGAGTGATGGAGGTTATGTCTGGGCATGCAAGAACTATGATGGAGATGTCCAGAGTGATTTCTTAGCTCAAGGTGTGTTATAATTTGAGCACTTGCCTTCAGCTCCTTGCTTTTGTTTTTAATTTTTTTTTCATTGGTTTCTTGGTTCAGGGAAATTACTGAAAAATTCCTACTTTGTCTGCCTTCTCTCTATTTAATTTCTAACTTTATGTGTTTGGTTTTAGGGTTTGGATCTCTTGGGTTGATGACTTCTGTATTGGTATGTTCCATTCTAGTCTTCAATACATCATGACTAAGACTAAGATTTACAATCTGCATGTCCTGCAATTCGCAGATTGAGTTTGATTTCTTAATTTTGTTTAATCTCTATGAAATTCATTTATATCTAAAAAAATAACATAAAAATTAAAAACACATGATTACATTTGTTTGTTTTACTTGCCATCATGAAAATGTAATCTTCAGTATCATTATAGGCCGTTAGTTCTTACTTTACTTTCATTGGAATCCCTTTCTACAGTTAGGCTCCCTTTTTTGTGGACCTAGTTTTTTTGTATACCCTTGGTACTTTTTATTCAGTTATTCATGAAAAAAGAAAGTATTATTTAATGTGCTCCAACTTGTTAGGTGGATATGCATTTTTACCATTGAAATTCATTTAGCCATTAAATTACCCAATTTTGTCAGTACTGAAGTGTATAAGGATTACTGTATCATCAAACAAGACTTCAATATTAATGTTTATCCTTGTTGAAATTTGAAGCATGTCTGCATTTTGAATTCAGGTTTGCCCTGATGGAAAGACAATTGAAGCTGAAGCAGCTCATGGTACTGTTACACGACATTATAGGGTTCATCAAAAAGGAGGTGAAACAAGCACAAACAGTATAGCTTCCATCTTTGCTTGGTCTCGTGGCCTTGCTCACAGGTAGATATCATTTGGTTACTATCTCTTTGATCTCTCAAACATTTTTAGGTCAATGGAAATTTTAAACATTAACTGTTTATCACTGTTTTAAAGGGCACGACTAGATGACAACGCTAGTCTCTTAGAGTTCACCGAGAAACTAGAATCGGCTTGTGTCGATACTGTGGAATCTGGGAAGATGACTAAGGATCTTGCTCTTATCATTCATGGATCCAAGTAAGTTCCATATTCGTAGTCATGAATGGCCTCTTTCCACTCAGCCGTTAAGATTGAAAATCTTATCTTTTTAACATTATACCCATGTTTGGTGCAGGCTTTCTCGAAATCATTATCTGAATACTGAAGAATTCATTGATGCTGTGGCGGAGGAACTAAAATCCAGACTTCTCAAGGCATAG

mRNA sequence

TATAAAAAAGGCGCCTGGTGCGGCGGCGGCTTTCATCCACCTACTGTATCTCTCCATTTTTTTTCTTGCTTCGATTTTTCCCTCTCTTTGCGCTCTCCTTATTCGATCTTCTCTGTGATTACTTCGTTTGAAGTGAGGGTGGTCAATAATCATGGCTTTTCAGAAGATCAAGGTCGCCAACCCCATCGTTGAGATGGACGGTGATGAAATGACTCGAGTTATTTGGGAATCAATTAAAAATAAGCTTATTTTCCCCTTCTTGGAATTGGACATCAAGTATTTTGACCTTGGCCTTCCACACCGTGATGCCACCGATGATAAAGTTACAATTGAAAGTGCAGAAGCTACTTTAAAGTACAATGTAGCAATCAAATGTGCAACAATTACTCCTGATGAAGCACGTGTTACGGAATTTGGCTTGAAGCAGATGTGGAAAAGTCCAAATGGGACGATCAGGAATATTCTGAATGGCACTGTCTTCAGAGAGCCAATTCTTTGCAAAAATGTTCCTCGTCTTGTACCAGGTTGGACGAAGCCAATCTGTATTGGCAGACATGCTTTTGGTGATCAATACAAAGCTACTGATACAGTTATCAAAGGACCTGGAAAATTGAAATTGGTTTTTGAGGGGCAAGAGACGCAAGAGATAGAGGTTTTCAACTTTACTGGTGCTGGAGGAGTAGCGTTGTCCATGTACAACACTGACGAGTCAATCCGCTCCTTCGCTGAGGCATCAATGAACACTGCTTATGAGAAGAAATGGCCTCTTTACCTTAGCACAAAAAATACTATTCTAAAGAAATATGATGGAAGATTCAAGGATATATTCCAAGAAGTATACGAATCCCAGTGGAAATCCAAGTTCGAGGCTGCTGGCATATGGTATGAACACCGTCTCATTGATGATATGGTGGCTTATGCACTTAAGAGTGATGGAGGTTATGTCTGGGCATGCAAGAACTATGATGGAGATGTCCAGAGTGATTTCTTAGCTCAAGGGTTTGGATCTCTTGGGTTGATGACTTCTGTATTGGTTTGCCCTGATGGAAAGACAATTGAAGCTGAAGCAGCTCATGGTACTGTTACACGACATTATAGGGTTCATCAAAAAGGAGGTGAAACAAGCACAAACAGTATAGCTTCCATCTTTGCTTGGTCTCGTGGCCTTGCTCACAGGGCACGACTAGATGACAACGCTAGTCTCTTAGAGTTCACCGAGAAACTAGAATCGGCTTGTGTCGATACTGTGGAATCTGGGAAGATGACTAAGGATCTTGCTCTTATCATTCATGGATCCAAGCTTTCTCGAAATCATTATCTGAATACTGAAGAATTCATTGATGCTGTGGCGGAGGAACTAAAATCCAGACTTCTCAAGGCATAG

Coding sequence (CDS)

ATGGCTTTTCAGAAGATCAAGGTCGCCAACCCCATCGTTGAGATGGACGGTGATGAAATGACTCGAGTTATTTGGGAATCAATTAAAAATAAGCTTATTTTCCCCTTCTTGGAATTGGACATCAAGTATTTTGACCTTGGCCTTCCACACCGTGATGCCACCGATGATAAAGTTACAATTGAAAGTGCAGAAGCTACTTTAAAGTACAATGTAGCAATCAAATGTGCAACAATTACTCCTGATGAAGCACGTGTTACGGAATTTGGCTTGAAGCAGATGTGGAAAAGTCCAAATGGGACGATCAGGAATATTCTGAATGGCACTGTCTTCAGAGAGCCAATTCTTTGCAAAAATGTTCCTCGTCTTGTACCAGGTTGGACGAAGCCAATCTGTATTGGCAGACATGCTTTTGGTGATCAATACAAAGCTACTGATACAGTTATCAAAGGACCTGGAAAATTGAAATTGGTTTTTGAGGGGCAAGAGACGCAAGAGATAGAGGTTTTCAACTTTACTGGTGCTGGAGGAGTAGCGTTGTCCATGTACAACACTGACGAGTCAATCCGCTCCTTCGCTGAGGCATCAATGAACACTGCTTATGAGAAGAAATGGCCTCTTTACCTTAGCACAAAAAATACTATTCTAAAGAAATATGATGGAAGATTCAAGGATATATTCCAAGAAGTATACGAATCCCAGTGGAAATCCAAGTTCGAGGCTGCTGGCATATGGTATGAACACCGTCTCATTGATGATATGGTGGCTTATGCACTTAAGAGTGATGGAGGTTATGTCTGGGCATGCAAGAACTATGATGGAGATGTCCAGAGTGATTTCTTAGCTCAAGGGTTTGGATCTCTTGGGTTGATGACTTCTGTATTGGTTTGCCCTGATGGAAAGACAATTGAAGCTGAAGCAGCTCATGGTACTGTTACACGACATTATAGGGTTCATCAAAAAGGAGGTGAAACAAGCACAAACAGTATAGCTTCCATCTTTGCTTGGTCTCGTGGCCTTGCTCACAGGGCACGACTAGATGACAACGCTAGTCTCTTAGAGTTCACCGAGAAACTAGAATCGGCTTGTGTCGATACTGTGGAATCTGGGAAGATGACTAAGGATCTTGCTCTTATCATTCATGGATCCAAGCTTTCTCGAAATCATTATCTGAATACTGAAGAATTCATTGATGCTGTGGCGGAGGAACTAAAATCCAGACTTCTCAAGGCATAG

Protein sequence

MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTIESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALSMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLESACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA
Homology
BLAST of CmaCh04G022730 vs. ExPASy Swiss-Prot
Match: Q9SRZ6 (Cytosolic isocitrate dehydrogenase [NADP] OS=Arabidopsis thaliana OX=3702 GN=CICDH PE=1 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 3.7e-219
Identity = 365/408 (89.46%), Postives = 391/408 (95.83%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAF+KIKVANPIVEMDGDEMTRVIW+SIK+KLI PF+ELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFEKIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEAT KYNVAIKCATITPDE RVTEFGLKQMW+SPNGTIRNILNGTVFREPI+CKNVP
Sbjct: 61  ESAEATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQ-EIEVFNFTGAGGVAL 180
           +LVPGWTKPICIGRHAFGDQY+ATD VIKGPGKL + FEG++ + E EVF FTG GGVA+
Sbjct: 121 KLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAM 180

Query: 181 SMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFE 240
           +MYNTDESIR+FA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WKSK++
Sbjct: 181 AMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYD 240

Query: 241 AAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 300
           AAGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG
Sbjct: 241 AAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 300

Query: 301 KTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLE 360
           KTIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAW+RGLAHRA+LDDNA LL+FTEKLE
Sbjct: 301 KTIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLE 360

Query: 361 SACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRL 408
           +ACV TVESGKMTKDLALIIHGSKLSR+ YLNTEEFIDAVA ELK RL
Sbjct: 361 AACVGTVESGKMTKDLALIIHGSKLSRDTYLNTEEFIDAVAAELKERL 408

BLAST of CmaCh04G022730 vs. ExPASy Swiss-Prot
Match: P50218 (Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 9.0e-218
Identity = 366/413 (88.62%), Postives = 388/413 (93.95%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           M F KIKV NPIVEMDGDEMTRVIW+SIK+KLI PFLELDIKYFDLGLPHRDATDDKVT+
Sbjct: 1   MTFDKIKVENPIVEMDGDEMTRVIWKSIKDKLICPFLELDIKYFDLGLPHRDATDDKVTV 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEAT KYNVAIKCATITPDEARV EF LK MW+SPNGTIRNILNGTVFREPI+CKN+P
Sbjct: 61  ESAEATQKYNVAIKCATITPDEARVKEFNLKSMWRSPNGTIRNILNGTVFREPIMCKNIP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVF--EG-QETQEIEVFNFTGAGGV 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVI+G GKLKLVF  EG  E  E EV+NFTGAGGV
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIQGAGKLKLVFVPEGTDEKTEFEVYNFTGAGGV 180

Query: 181 ALSMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSK 240
           ALSMYNTDES+RSFAEASMN AY+KKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WKSK
Sbjct: 181 ALSMYNTDESVRSFAEASMNMAYQKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSK 240

Query: 241 FEAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 300
           +E AGIWYEHRLIDDM AYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP
Sbjct: 241 YEEAGIWYEHRLIDDMAAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 300

Query: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEK 360
           DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAW+RGLAHRA LD+N  LL+FTEK
Sbjct: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWTRGLAHRATLDNNERLLDFTEK 360

Query: 361 LESACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           LE+AC+  VESGKMTKDLALIIHGSKLSR+HYLNTEEFIDAVA+ELK+RLLKA
Sbjct: 361 LEAACIGAVESGKMTKDLALIIHGSKLSRDHYLNTEEFIDAVADELKARLLKA 413

BLAST of CmaCh04G022730 vs. ExPASy Swiss-Prot
Match: Q06197 (Isocitrate dehydrogenase [NADP] OS=Glycine max OX=3847 GN=IDH1 PE=1 SV=2)

HSP 1 Score: 756.1 bits (1951), Expect = 2.0e-217
Identity = 368/409 (89.98%), Postives = 387/409 (94.62%), Query Frame = 0

Query: 2   AFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTIE 61
           AFQKIKVANPIVEMDGDEMTRVIW+SIK+KLI PFLELDIKY+DLGLP+RD TDDKVTIE
Sbjct: 3   AFQKIKVANPIVEMDGDEMTRVIWKSIKDKLILPFLELDIKYYDLGLPYRDETDDKVTIE 62

Query: 62  SAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVPR 121
           SAEATLKYNVAIKCATITPDEARV EFGLK MWKSPNGTIRNILNGTVFREPILCKN+PR
Sbjct: 63  SAEATLKYNVAIKCATITPDEARVKEFGLKSMWKSPNGTIRNILNGTVFREPILCKNIPR 122

Query: 122 LVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVF--EGQ-ETQEIEVFNFTGAGGVA 181
           LVPGWTK ICIGRHAFGDQY+ATDTVIKG GKLKLVF  EGQ E  E EVFNFTG GGV+
Sbjct: 123 LVPGWTKAICIGRHAFGDQYRATDTVIKGAGKLKLVFVPEGQGEETEFEVFNFTGEGGVS 182

Query: 182 LSMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKF 241
           L+MYNTDESIRSFAEASM TA EKKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WKSKF
Sbjct: 183 LAMYNTDESIRSFAEASMATALEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKF 242

Query: 242 EAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPD 301
           EAAGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPD
Sbjct: 243 EAAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPD 302

Query: 302 GKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKL 361
           GKTIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAW+RGLAHRA+LDDNA LL+FTEKL
Sbjct: 303 GKTIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKL 362

Query: 362 ESACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRL 408
           E+AC+  VE+GKMTKDLALI+HGSKLSR HYLNTEEFIDAVA EL +RL
Sbjct: 363 EAACIGVVEAGKMTKDLALILHGSKLSREHYLNTEEFIDAVAAELSARL 411

BLAST of CmaCh04G022730 vs. ExPASy Swiss-Prot
Match: Q40345 (Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 2.6e-217
Identity = 361/410 (88.05%), Postives = 392/410 (95.61%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           M FQKIKVANPIVEMDGDEMTR+IW+ IK+KLIFPF+ELDIKYFDLGLP+RD T+DKVT+
Sbjct: 22  MGFQKIKVANPIVEMDGDEMTRIIWKYIKDKLIFPFVELDIKYFDLGLPYRDETNDKVTV 81

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARV EFGLK MW+SPNGTIRNILNGTVFREPI+CKN+P
Sbjct: 82  ESAEATLKYNVAIKCATITPDEARVKEFGLKSMWRSPNGTIRNILNGTVFREPIICKNIP 141

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVF--EGQ-ETQEIEVFNFTGAGGV 180
           RL+PGWTKPICIGRHAFGDQY+ATD+VIKGPGKLKLVF  EGQ ET ++EV+NFTG GGV
Sbjct: 142 RLIPGWTKPICIGRHAFGDQYRATDSVIKGPGKLKLVFVPEGQGETTDLEVYNFTGEGGV 201

Query: 181 ALSMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSK 240
           AL+MYNTDESIRSFAEASM  A EKKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WKSK
Sbjct: 202 ALAMYNTDESIRSFAEASMAVALEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEAGWKSK 261

Query: 241 FEAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 300
           +EAAGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP
Sbjct: 262 YEAAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 321

Query: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEK 360
           DGKTIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAW+RGLAHRA+LDDNA+LL+FTEK
Sbjct: 322 DGKTIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNATLLDFTEK 381

Query: 361 LESACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRL 408
           LE+AC+  VESGKMTKDLALI+HGSKLSR HYLNTEEFIDAVA ELK+++
Sbjct: 382 LEAACIGVVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVAAELKTKI 431

BLAST of CmaCh04G022730 vs. ExPASy Swiss-Prot
Match: P50217 (Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum OX=4113 GN=ICDH-1 PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.3e-216
Identity = 367/414 (88.65%), Postives = 388/414 (93.72%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKI V NPIVEMDGDEMTRVIW+SIK+KLI PFLELDIKYF LGLPHRDATDDKVT+
Sbjct: 1   MAFQKITVQNPIVEMDGDEMTRVIWKSIKDKLILPFLELDIKYFSLGLPHRDATDDKVTV 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEAT KYNVAIKCATITPDEARV EF LK MW+SPNGTIRNILNGTVFREPI+CKN+P
Sbjct: 61  ESAEATQKYNVAIKCATITPDEARVKEFNLKSMWRSPNGTIRNILNGTVFREPIMCKNIP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVF--EG-QETQEIEVFNFTGAGGV 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVIKG GKLKLVF  EG  E  E EV+NFTGAGGV
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIKGAGKLKLVFVPEGSDEKTEFEVYNFTGAGGV 180

Query: 181 ALSMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSK 240
           ALSMYNTDES+RSFAEASMN A++KKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WKSK
Sbjct: 181 ALSMYNTDESVRSFAEASMNMAFQKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSK 240

Query: 241 FEAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 300
           +E AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP
Sbjct: 241 YEEAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 300

Query: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEK 360
           DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAW+RGLAHRA LD+N  LL+FTEK
Sbjct: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWTRGLAHRATLDNNERLLDFTEK 360

Query: 361 LESACVDTVESGKMTKDLAL-IIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           LE+AC+  VESGKMTKDLAL IIHGSKLSR HYLNTEEFIDAVA+ELK+RLLKA
Sbjct: 361 LEAACIGAVESGKMTKDLALIIIHGSKLSREHYLNTEEFIDAVADELKARLLKA 414

BLAST of CmaCh04G022730 vs. ExPASy TrEMBL
Match: A0A6J1JPW4 (Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111487821 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 6.7e-240
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES
Sbjct: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA
Sbjct: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. ExPASy TrEMBL
Match: A0A6J1GUM5 (Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111457689 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.3e-239
Identity = 409/410 (99.76%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKV NPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVDNPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES
Sbjct: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA
Sbjct: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. ExPASy TrEMBL
Match: A0A6J1JZB7 (Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111489234 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.3e-235
Identity = 398/410 (97.07%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARV EFGLKQMW+SPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWRSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVIKGPGKLKL+FEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIKGPGKLKLIFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAWSRGLAHRA+LDDNASLL+FTEKLES
Sbjct: 301 TIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNASLLDFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           AC++TVESGKMTKDLALIIHGSKLSRNHYLNTEEF+DAVAEELKSRLLKA
Sbjct: 361 ACINTVESGKMTKDLALIIHGSKLSRNHYLNTEEFVDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. ExPASy TrEMBL
Match: A0A6J1FJA0 (Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111444771 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.3e-235
Identity = 399/410 (97.32%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARV EFGLKQMW+SPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWRSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAWSRGLAHRA+LDDNASLL+FTEKLES
Sbjct: 301 TIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNASLLDFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           AC+ TVESGKMTKDLALIIHGSKLSRNHYLNTEEF+DAVAEELKSRLLKA
Sbjct: 361 ACISTVESGKMTKDLALIIHGSKLSRNHYLNTEEFVDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. ExPASy TrEMBL
Match: A0A6J1JRV9 (Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111489234 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.3e-235
Identity = 398/410 (97.07%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARV EFGLKQMW+SPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWRSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVIKGPGKLKL+FEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIKGPGKLKLIFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAWSRGLAHRA+LDDNASLL+FTEKLES
Sbjct: 301 TIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNASLLDFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           AC++TVESGKMTKDLALIIHGSKLSRNHYLNTEEF+DAVAEELKSRLLKA
Sbjct: 361 ACINTVESGKMTKDLALIIHGSKLSRNHYLNTEEFVDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. NCBI nr
Match: XP_022991136.1 (cytosolic isocitrate dehydrogenase [NADP]-like [Cucurbita maxima] >XP_022991137.1 cytosolic isocitrate dehydrogenase [NADP]-like [Cucurbita maxima] >KAG6602013.1 Cytosolic isocitrate dehydrogenase [NADP], partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 839.3 bits (2167), Expect = 1.4e-239
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES
Sbjct: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA
Sbjct: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. NCBI nr
Match: XP_022955812.1 (cytosolic isocitrate dehydrogenase [NADP]-like [Cucurbita moschata] >XP_022955820.1 cytosolic isocitrate dehydrogenase [NADP]-like [Cucurbita moschata])

HSP 1 Score: 837.0 bits (2161), Expect = 6.8e-239
Identity = 409/410 (99.76%), Postives = 409/410 (99.76%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKV NPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVDNPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES
Sbjct: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA
Sbjct: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. NCBI nr
Match: KAG7032706.1 (Cytosolic isocitrate dehydrogenase [NADP] [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 836.6 bits (2160), Expect = 8.9e-239
Identity = 408/410 (99.51%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIR+FAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRAFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFT+KLES
Sbjct: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTKKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA
Sbjct: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. NCBI nr
Match: XP_022938576.1 (cytosolic isocitrate dehydrogenase [NADP] isoform X2 [Cucurbita moschata] >XP_022938578.1 cytosolic isocitrate dehydrogenase [NADP] isoform X2 [Cucurbita moschata] >XP_023550725.1 cytosolic isocitrate dehydrogenase [NADP] isoform X2 [Cucurbita pepo subsp. pepo] >XP_023550726.1 cytosolic isocitrate dehydrogenase [NADP] isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.1 bits (2130), Expect = 2.7e-235
Identity = 399/410 (97.32%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARV EFGLKQMW+SPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWRSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAWSRGLAHRA+LDDNASLL+FTEKLES
Sbjct: 301 TIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNASLLDFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           AC+ TVESGKMTKDLALIIHGSKLSRNHYLNTEEF+DAVAEELKSRLLKA
Sbjct: 361 ACISTVESGKMTKDLALIIHGSKLSRNHYLNTEEFVDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. NCBI nr
Match: XP_022938575.1 (cytosolic isocitrate dehydrogenase [NADP] isoform X1 [Cucurbita moschata] >XP_023550724.1 cytosolic isocitrate dehydrogenase [NADP] isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.1 bits (2130), Expect = 2.7e-235
Identity = 399/410 (97.32%), Postives = 408/410 (99.51%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEATLKYNVAIKCATITPDEARV EFGLKQMW+SPNGTIRNILNGTVFREPILCKNVP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWRSPNGTIRNILNGTVFREPILCKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180
           RLVPGWTKPICIGRHAFGDQY+ATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDTVIKGPGKLKLVFEGQETQEIEVFNFTGAGGVALS 180

Query: 181 MYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240
           MYNTDESIRSFA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA
Sbjct: 181 MYNTDESIRSFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEA 240

Query: 241 AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300
           AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 241 AGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 300

Query: 301 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLES 360
           TIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAWSRGLAHRA+LDDNASLL+FTEKLES
Sbjct: 301 TIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNASLLDFTEKLES 360

Query: 361 ACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLLKA 411
           AC+ TVESGKMTKDLALIIHGSKLSRNHYLNTEEF+DAVAEELKSRLLKA
Sbjct: 361 ACISTVESGKMTKDLALIIHGSKLSRNHYLNTEEFVDAVAEELKSRLLKA 410

BLAST of CmaCh04G022730 vs. TAIR 10
Match: AT1G65930.1 (cytosolic NADP+-dependent isocitrate dehydrogenase )

HSP 1 Score: 761.9 bits (1966), Expect = 2.6e-220
Identity = 365/408 (89.46%), Postives = 391/408 (95.83%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           MAF+KIKVANPIVEMDGDEMTRVIW+SIK+KLI PF+ELDIKYFDLGLPHRDATDDKVTI
Sbjct: 1   MAFEKIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           ESAEAT KYNVAIKCATITPDE RVTEFGLKQMW+SPNGTIRNILNGTVFREPI+CKNVP
Sbjct: 61  ESAEATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFEGQETQ-EIEVFNFTGAGGVAL 180
           +LVPGWTKPICIGRHAFGDQY+ATD VIKGPGKL + FEG++ + E EVF FTG GGVA+
Sbjct: 121 KLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFEGKDGKTETEVFTFTGEGGVAM 180

Query: 181 SMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFE 240
           +MYNTDESIR+FA+ASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WKSK++
Sbjct: 181 AMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYD 240

Query: 241 AAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 300
           AAGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG
Sbjct: 241 AAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 300

Query: 301 KTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLE 360
           KTIEAEAAHGTVTRH+RVHQKGGETSTNSIASIFAW+RGLAHRA+LDDNA LL+FTEKLE
Sbjct: 301 KTIEAEAAHGTVTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLE 360

Query: 361 SACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRL 408
           +ACV TVESGKMTKDLALIIHGSKLSR+ YLNTEEFIDAVA ELK RL
Sbjct: 361 AACVGTVESGKMTKDLALIIHGSKLSRDTYLNTEEFIDAVAAELKERL 408

BLAST of CmaCh04G022730 vs. TAIR 10
Match: AT1G54340.1 (isocitrate dehydrogenase )

HSP 1 Score: 729.6 bits (1882), Expect = 1.4e-210
Identity = 348/411 (84.67%), Postives = 384/411 (93.43%), Query Frame = 0

Query: 1   MAFQKIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTI 60
           M F+KIKV NP+VEMDGDEMTRVIW+ IK+KLIFPFLELDIKYFDLGLP+RD TDDKVTI
Sbjct: 1   MEFEKIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTI 60

Query: 61  ESAEATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVP 120
           E+AEATLKYNVAIKCATITPDEARV EFGLK+MW+SPNGTIRNILNGTVFREPI+C+N+P
Sbjct: 61  ETAEATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIP 120

Query: 121 RLVPGWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVFE---GQETQEIEVFNFTGAGGV 180
           RLVPGWTKPICIGRHAFGDQY+ATD ++  PGKLKLVFE     +  E EVFNFTG GGV
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTG-GGV 180

Query: 181 ALSMYNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSK 240
           AL+MYNTDESIR+FAE+SM TAY+KKWPLYLSTKNTILK YDGRFKDIFQEVYE+ W+SK
Sbjct: 181 ALAMYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQEVYEANWRSK 240

Query: 241 FEAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 300
           +EAAGIWYEHRLIDDMVAYA+KS+GGYVWACKNYDGDVQSDFLAQG+GSLG+MTSVLVCP
Sbjct: 241 YEAAGIWYEHRLIDDMVAYAMKSEGGYVWACKNYDGDVQSDFLAQGYGSLGMMTSVLVCP 300

Query: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEK 360
           DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRA+LD NA+LL +TEK
Sbjct: 301 DGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDSNAALLSYTEK 360

Query: 361 LESACVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSRLL 409
           LE+AC+ TVESGKMTKDLAL+IHG+K+ R+ Y+NTEEFIDAVA ELK RLL
Sbjct: 361 LEAACMGTVESGKMTKDLALLIHGAKVRRDQYVNTEEFIDAVAWELKRRLL 410

BLAST of CmaCh04G022730 vs. TAIR 10
Match: AT5G14590.1 (Isocitrate/isopropylmalate dehydrogenase family protein )

HSP 1 Score: 666.0 bits (1717), Expect = 1.9e-191
Identity = 317/405 (78.27%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 5   KIKVANPIVEMDGDEMTRVIWESIKNKLIFPFLELDIKYFDLGLPHRDATDDKVTIESAE 64
           +I+V NPIVEMDGDEMTRVIW  IK KLI P+L+LDIKYFDLG+ +RDATDDKVT+ESAE
Sbjct: 75  RIQVQNPIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAE 134

Query: 65  ATLKYNVAIKCATITPDEARVTEFGLKQMWKSPNGTIRNILNGTVFREPILCKNVPRLVP 124
           A LKYNVAIKCATITPDE RV EFGLK MW+SPNGTIRNIL+GTVFREPI+C N+PRLVP
Sbjct: 135 AALKYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVP 194

Query: 125 GWTKPICIGRHAFGDQYKATDTVIKGPGKLKLVF---EGQETQEIEVFNFTGAGGVALSM 184
           GW KPICIGRHAFGDQY+ATDTVIKGPGKLK+VF   +G    E++V++F G  GVAL+M
Sbjct: 195 GWEKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGP-GVALAM 254

Query: 185 YNTDESIRSFAEASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYESQWKSKFEAA 244
           YN DESIR+FAE+SM  A  KKWPLYLSTKNTILKKYDGRFKDIFQEVYE+ WK KFE  
Sbjct: 255 YNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEEH 314

Query: 245 GIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKT 304
            IWYEHRLIDDMVAYA+KS+GGYVWACKNYDGDVQSD LAQGFGSLGLMTSVL+  DGKT
Sbjct: 315 SIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLLSADGKT 374

Query: 305 IEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRARLDDNASLLEFTEKLESA 364
           +E+EAAHGTVTRH+R+HQKG ETSTNSIASIFAW+RGL HRA+LD N  L++F +KLES+
Sbjct: 375 LESEAAHGTVTRHFRLHQKGQETSTNSIASIFAWTRGLEHRAKLDKNEKLMDFVKKLESS 434

Query: 365 CVDTVESGKMTKDLALIIHGSKLSRNHYLNTEEFIDAVAEELKSR 407
           CV+TVE+GKMTKDLAL+IHG K+SR+ +LNTEEFIDAVA +LK++
Sbjct: 435 CVNTVETGKMTKDLALLIHGPKVSRDLFLNTEEFIDAVASKLKTQ 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRZ63.7e-21989.46Cytosolic isocitrate dehydrogenase [NADP] OS=Arabidopsis thaliana OX=3702 GN=CIC... [more]
P502189.0e-21888.62Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q061972.0e-21789.98Isocitrate dehydrogenase [NADP] OS=Glycine max OX=3847 GN=IDH1 PE=1 SV=2[more]
Q403452.6e-21788.05Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa OX=... [more]
P502171.3e-21688.65Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum OX=4113 GN=ICDH-1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JPW46.7e-240100.00Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111487821 PE=3... [more]
A0A6J1GUM53.3e-23999.76Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111457689 PE... [more]
A0A6J1JZB71.3e-23597.07Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111489234 PE=3... [more]
A0A6J1FJA01.3e-23597.32Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111444771 PE... [more]
A0A6J1JRV91.3e-23597.07Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111489234 PE=3... [more]
Match NameE-valueIdentityDescription
XP_022991136.11.4e-239100.00cytosolic isocitrate dehydrogenase [NADP]-like [Cucurbita maxima] >XP_022991137.... [more]
XP_022955812.16.8e-23999.76cytosolic isocitrate dehydrogenase [NADP]-like [Cucurbita moschata] >XP_02295582... [more]
KAG7032706.18.9e-23999.51Cytosolic isocitrate dehydrogenase [NADP] [Cucurbita argyrosperma subsp. argyros... [more]
XP_022938576.12.7e-23597.32cytosolic isocitrate dehydrogenase [NADP] isoform X2 [Cucurbita moschata] >XP_02... [more]
XP_022938575.12.7e-23597.32cytosolic isocitrate dehydrogenase [NADP] isoform X1 [Cucurbita moschata] >XP_02... [more]
Match NameE-valueIdentityDescription
AT1G65930.12.6e-22089.46cytosolic NADP+-dependent isocitrate dehydrogenase [more]
AT1G54340.11.4e-21084.67isocitrate dehydrogenase [more]
AT5G14590.11.9e-19178.27Isocitrate/isopropylmalate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024084Isopropylmalate dehydrogenase-like domainSMARTSM01329Iso_dh_2coord: 11..399
e-value: 1.2E-123
score: 426.7
IPR024084Isopropylmalate dehydrogenase-like domainPFAMPF00180Iso_dhcoord: 7..399
e-value: 1.7E-93
score: 313.6
NoneNo IPR availableGENE3D3.40.718.10Isopropylmalate Dehydrogenasecoord: 3..410
e-value: 7.2E-189
score: 629.6
NoneNo IPR availablePIRSRPIRSR000108-4PIRSR000108-4coord: 7..362
e-value: 2.0E-198
score: 656.3
NoneNo IPR availablePIRSRPIRSR000108-2PIRSR000108-2coord: 7..362
e-value: 2.0E-198
score: 656.3
NoneNo IPR availablePIRSRPIRSR000108-3PIRSR000108-3coord: 7..362
e-value: 2.0E-198
score: 656.3
NoneNo IPR availablePIRSRPIRSR000108-1PIRSR000108-1coord: 7..362
e-value: 2.0E-198
score: 656.3
NoneNo IPR availablePANTHERPTHR11822:SF34ISOCITRATE DEHYDROGENASE [NADP]coord: 1..410
NoneNo IPR availableSUPERFAMILY53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 3..407
IPR004790Isocitrate dehydrogenase NADP-dependentPIRSFPIRSF000108IDH_NADPcoord: 1..410
e-value: 9.0E-227
score: 750.3
IPR004790Isocitrate dehydrogenase NADP-dependentTIGRFAMTIGR00127TIGR00127coord: 4..407
e-value: 4.7E-241
score: 797.4
IPR004790Isocitrate dehydrogenase NADP-dependentPANTHERPTHR11822NADP-SPECIFIC ISOCITRATE DEHYDROGENASEcoord: 1..410
IPR019818Isocitrate/isopropylmalate dehydrogenase, conserved sitePROSITEPS00470IDH_IMDHcoord: 270..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G022730.1CmaCh04G022730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006102 isocitrate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0043229 intracellular organelle
molecular_function GO:0004450 isocitrate dehydrogenase (NADP+) activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor