CmaCh04G014580 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G014580
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr04: 7415195 .. 7418522 (-)
RNA-Seq Expression	CmaCh04G014580
Synteny	CmaCh04G014580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCAGCTCCCTTCATTTTCCTTTTCCTCCTCTCTCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGCTCCATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAGGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTTGATGAGGAACTGATGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTATCGGCACCGCTGTTGAGCGCGGGGGAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAGCCAGAAGGGAGACCGCGAGGGAGGGAATTTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTGATCAAGGTACGATTCGATATGGCGCCGTTGTTATTTGGTCTCTGTTTGTCTGTTCGTTCGGTTACACGGACCATTACATTTGCTTTATTCAATGCATCAAAAAATTAGGCGAAAATAAGTTTTTGTTTGGATGTGGCCACTAAAATATATATATATTTTAAAATTTAATCTAATAAATAATAATGTTATATTTTTTTCAAAGTAATAGTAAAATAATAAATCATTAACAAATAAGCTTCTTCTTTAAGAATTAATCTTACAAATAAAATTGTTATTAAAATGGGTAATCAACCTTGTAATTAATGCTTCAAAAGTGCATTAAATTCTCCCATAATGACATTATTACTGAGCAGAAATCACAATTGATGGTAGCATTAAGGGGAATTAGTGAGAGAAAAAACATTTAAGTATTCATAGTTATATAATTTTAAAACTATGCTTTAATTTAATTTCATTCACATTTAATTGATTTCTGATTTCTTTCCAATTACTTATGAATTACTTACATTGGAACATCATCTTTATACAAAGTGAGGAATTTAACTCTTGATGAAGAACCTGTCCTAACTATAGTTTAATATTTTAATTTTTACCAATATGATCAGTAAAAACAGTATATTAAATAAATAACAAAAATGTTTTTTAGTGGAAAAAAAGATTATATATATATATATTATATATATTATATTTGTTCATTAAATTTATGTTAAATACGTTGATAATTTTGTTGGTATCACGGGAGAGATTCTAGGATTCCCAAGCTCCACCAAATGCATGTTACAAATTCAGATGTGGATTCTCCTCATAATTTTTTAACTTGCCTTTTTTCTAATTGTTTTATTCCTATCATTTGCCTTTTATTATATATATATATATATATATATATATAGATGTATATGTATATATATGTATATGTACATGTATGTATATGTATATGTACTTTCATGTATATGTGTGTGCGTATACATGTATATGTATATATATGTATATGCATATGTACTTTCATGTATATGTGTGCGTGTATATATATATGTATGTATGTATGTATGTATATGTATATGTATATGAATATGAATATGGGCTGGCAGCCGGACCAGCTAAAAGCAACTCCAAACTCCGATGTCATAGGTTCAAATTATACAGAGCGTGGATTCGATTGTTGTAATCGAATGATTTCTTTAAACTCTGTCTAGTATAAACTAAGTAAAAAAATCTTGCAACAAGTCTTGATGTATACAAAACGCACTAGGTTTTTGTCTTTGGGTACTACGGACCCTCTGCCATCTATTGCAGCAGAGCCGTTTCATGAGCGGAGGCTAAGACGAGAAAGAAGAGGCTTTCCCGTGGACTAGCGTGGGCTAAGCTGGGAGAGTTGATATATATATCAAAACTAGTCTTGGAATTTAAGACAACTAAGGGGCAATTGTTCCTTTTTCTTACCTGATCTGCTCAAGTTCTTTTTAGTATAAATTAGTTGCTGATTTTTGGAGGAGATTGCATTTTTTCCCTATTAAATTAACAATGATCTTAAGAGTTTGAATGAACAGGGACTACATTCAATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGGAAGGAAGCGCATGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATTGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATATGTTGTCGTCCCCAAGTACAAAACCAATATAATGCTCATCGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTTGTTGATGGTTGGACGACTTACAATTCCTCTACCTTTAGTAAGCAATTCACAAATCAGTTTAAAATTTATAATATTGTCGTATCAAAATTATTCCGAAATTGGTAGCTGAATGTTTGGGATATATGTTGGCAGCTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCTTAAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTTTTCCAGCAATGCAATTTATACGCTAGAAAGCCACTCCCCAACCAAAAGAACGCCTTCACTGCACAGGGCCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCATAACTGCACCATCACGGCTGCCCCAAACTGGGTGATGGATTTGAATGAGACGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGCTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACGAGCTTGAGGGTCGATTGGGATGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACTATGGGAGATACCTGGCTGCCCTATACAGATATCCCCTTTTCTGGAGGTTTGCTATGA

mRNA sequence

ATGAACTCAGCTCCCTTCATTTTCCTTTTCCTCCTCTCTCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGCTCCATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAGGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTTGATGAGGAACTGATGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTATCGGCACCGCTGTTGAGCGCGGGGGAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAGCCAGAAGGGAGACCGCGAGGGAGGGAATTTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTGATCAAGGGACTACATTCAATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGGAAGGAAGCGCATGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATTGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATATGTTGTCGTCCCCAAGTACAAAACCAATATAATGCTCATCGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTTGTTGATGGTTGGACGACTTACAATTCCTCTACCTTTACTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCTTAAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTTTTCCAGCAATGCAATTTATACGCTAGAAAGCCACTCCCCAACCAAAAGAACGCCTTCACTGCACAGGGCCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCATAACTGCACCATCACGGCTGCCCCAAACTGGGTGATGGATTTGAATGAGACGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGCTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACGAGCTTGAGGGTCGATTGGGATGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACTATGGGAGATACCTGGCTGCCCTATACAGATATCCCCTTTTCTGGAGGTTTGCTATGA

Coding sequence (CDS)

ATGAACTCAGCTCCCTTCATTTTCCTTTTCCTCCTCTCTCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGCTCCATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAGGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTTGATGAGGAACTGATGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTATCGGCACCGCTGTTGAGCGCGGGGGAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAGCCAGAAGGGAGACCGCGAGGGAGGGAATTTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTGATCAAGGGACTACATTCAATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGGAAGGAAGCGCATGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATTGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATATGTTGTCGTCCCCAAGTACAAAACCAATATAATGCTCATCGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTTGTTGATGGTTGGACGACTTACAATTCCTCTACCTTTACTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCTTAAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTTTTCCAGCAATGCAATTTATACGCTAGAAAGCCACTCCCCAACCAAAAGAACGCCTTCACTGCACAGGGCCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCATAACTGCACCATCACGGCTGCCCCAAACTGGGTGATGGATTTGAATGAGACGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGCTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGACCAGGAGCTAACACGAGCTTGAGGGTCGATTGGGATGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACTATGGGAGATACCTGGCTGCCCTATACAGATATCCCCTTTTCTGGAGGTTTGCTATGA

Protein sequence

MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL

Homology

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BLAST of CmaCh04G014580 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.2e-191
Identity = 329/550 (59.82%), Postives = 420/550 (76.36%), Query Frame = 0


Query: 20  SDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYL 79
           S S  PS + CKST YPKLCR+IL++++ SPSDPY YGKF++++CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAI 139
            R          +E+GA+ DC ELS+ +V +L+++   L+ A+ +   L+E + S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGDREG 199
           VTN QTC+DGLVE++S    A+ +P+ +   LYS+SLGLV ++L+R  K+ ++ KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTT---GILINNTVVVSS 259
           G     +  REPL TLIKGL    +C+    C    R L +LG T    IL++  V+V  
Sbjct: 254 G---GNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 260 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTV 319
             +DNFT+I DAI+ APNN+ P+DGYFVIY R+G Y+EY+VVP  K N+ML+GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 320 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 379
           ITGNHNVVDGWTTYN S+F V G+ F+A+D+TFRNTAG EKHQAVA+RN+A+ S+FYRCS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 380 FEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 439
           FEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 440 TDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGW 499
            DPNQ+TGISI NCTI AAP+   +     T +LGRPWKP+SRTV+MQSYI D++ PVGW
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAMT-FLGRPWKPYSRTVFMQSYISDIVQPVGW 553

Query: 500 LEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPY 559
           LEWNGT+GLDT+YYGE+ N+GPGANT+ RV W G++L+N+ EAMNFTVYNFTMGDTWLP 
Sbjct: 554 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 613

Query: 560 TDIPFSGGLL 564
           TDIPF GGLL
Sbjct: 614 TDIPFYGGLL 616

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BLAST of CmaCh04G014580 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 2.9e-190
Identity = 331/549 (60.29%), Postives = 413/549 (75.23%), Query Frame = 0


Query: 22  SPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYLDR 81
           SP P  + CKST YPKLCR+ILS+++ SPSDPY YGKF++++CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAIVT 141
                     +EV A+ DC EL++ +VE+L+++   L+AAE +   L++R+ S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGD-REGG 201
           N QTC+DGLV+++S    A+  PL +   LYSVSLGLV ++L+R  K+ +  KG    GG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 NFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTT---GILINNTVVVSST 261
           N P     REPL TLIK L    +C++   C    R L +LG T    IL+   V V   
Sbjct: 260 NKPV----REPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 262 GADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVI 321
             DNF +I +A++ APN++ P+ GYFVIY R G Y+EYVV+   K NIMLIGDGIN+T+I
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 322 TGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSF 381
           +GNH+ +DGWTTYNSSTF V GD FVA+D+TFRNTAG EKHQAVAVRN+AD STFYRCSF
Sbjct: 380 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 439

Query: 382 EGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRT 441
           EGYQDTLYVHSLRQFYRECD+YGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRT
Sbjct: 440 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 499

Query: 442 DPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 501
           DPNQ TGISI NCTI AAP+   D   T T +LGRPWKP+SRTVY+QSYI D++ PVGWL
Sbjct: 500 DPNQKTGISIINCTIGAAPDLAADPKSTMT-FLGRPWKPYSRTVYIQSYISDVVQPVGWL 559

Query: 502 EWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYT 561
           EWNGT GLDT+ YGE++N+GPGA+TS RV W G+SL+N+ +AMNFTVYNFT+GDTWLP T
Sbjct: 560 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQT 619

Query: 562 DIPFSGGLL 564
           DIPF GGLL
Sbjct: 620 DIPFYGGLL 621

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BLAST of CmaCh04G014580 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 502.3 bits (1292), Expect = 7.2e-141
Identity = 273/570 (47.89%), Postives = 367/570 (64.39%), Query Frame = 0


Query: 7   IFLFLLSLRLAGASDSPP-PSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCL 66
           +FL L +L +A  +  P   S+ +CK+T  PK C+S+    + S  D   YG+FS+RK L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQS---IDGVLEAAEGV 126
            Q+ K +  I  YL R  ++ L++  VGAL+DCR L+    ++L +      +  +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEELMERIESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLLSAGELYSVSLGLVKN 186
                + I+++LSA +TN QTC+DG+  + SS   + N ++ PL++  +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLAL--- 190

Query: 187 SLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQS----TKCSGRKR 246
             ++ W  KK+ Q       +        +P      G   +     +       S RK 
Sbjct: 191 -FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 250

Query: 247 MLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV 306
              D     +L+++ V V+  G  NFT+I +A++ APN +    GYFVIYV  G Y+E V
Sbjct: 251 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 310

Query: 307 VVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAE 366
           V+ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA++MTFRNTAG E
Sbjct: 311 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 370

Query: 367 KHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQC 426
           KHQAVA+R+SADLS FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ C
Sbjct: 371 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 430

Query: 427 NLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKP 486
           NLY R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTI  A + V   N T   YLGRPWK 
Sbjct: 431 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKTYLGRPWKE 490

Query: 487 FSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNI 546
           +SRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV W G+ ++N 
Sbjct: 491 YSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINS 550

Query: 547 TEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           T+A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 TDANNFTVENFLLGDGWMVQSGVPYISGLL 572

[Close]

BLAST of CmaCh04G014580 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 265/587 (45.14%), Postives = 361/587 (61.50%), Query Frame = 0


Query: 4   APFIFLFLLSLRL---AGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 63
           +P   L  LS  L     +S +   S+ +CK+T  PK C+S+      S  +   YG FS
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFS 62

Query: 64  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEV-GALEDCRELSDSNVEF-LQSIDGVLEA 123
           +RK L Q+ K    +  Y+ R  ++ L++  V  AL+DCR L+   +++ L S + V + 
Sbjct: 63  IRKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDT 122

Query: 124 AEGVDEELM-----ERIESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLLSAGE 183
           +     + +     + I+++LSA +TN QTC++GL  + S     ++   ++ PL++  +
Sbjct: 123 SAKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTK 182

Query: 184 LYSVSLGLVKNSLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQST 243
           L  VSL L     ++ W  KK+ + G         ++   +P      G   +    + T
Sbjct: 183 LLGVSLAL----FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPL-KMTEKT 242

Query: 244 KC---SGRKRMLTDLGTTG-------ILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQ 303
           K    S  +R L D  + G       +LI++ V VS  G  NFT+I  A++ APNN+   
Sbjct: 243 KAVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGS 302

Query: 304 DGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYG 363
            G+F+IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V  
Sbjct: 303 AGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTA 362

Query: 364 DGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVY 423
             FVA+++TFRNTAG EKHQAVA+R+ AD S FY CSFE YQDTLY HSLRQFYRECDVY
Sbjct: 363 PNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVY 422

Query: 424 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWV 483
           GTVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTI  A + V
Sbjct: 423 GTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV 482

Query: 484 MDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPG 543
              N T   YLGRPWK +SRTVYMQSYI   + PVGW EWNG   L TLYY E+ N GPG
Sbjct: 483 SS-NYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPG 542

Query: 544 ANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           +NT+ RV W G+ ++N T+A NFTV    +   W+  T +P++ GL+
Sbjct: 543 SNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

[Close]

BLAST of CmaCh04G014580 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 463.8 bits (1192), Expect = 2.8e-129
Identity = 252/558 (45.16%), Postives = 337/558 (60.39%), Query Frame = 0


Query: 12  LSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKM 71
           +S ++    ++  PS + C+    P  CRS+L +    P D Y YG+ S+R+ L +A + 
Sbjct: 22  ISAQIPAIGNATSPSNI-CRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRF 81

Query: 72  SSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIE 131
            S+I   LDR G     +  VGALEDC+ L+   +++L S     ++ + +     E + 
Sbjct: 82  ISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVH 141

Query: 132 SILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKKRSQK 191
           + LSA +TN QTC++GL  + S   N LS  L +  +LY VSL L     S+ W  R Q+
Sbjct: 142 TFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQR 201

Query: 192 G------DREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTGILI 251
                        F      + PL         M    ++   +  +R L       + +
Sbjct: 202 SRPIWQPQARFKKFFGFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQV 261

Query: 252 NNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLI 311
           ++ V V   G  NFT+I  AI+ APN +   +GYF+IYV  G Y+EYV VPK K  +M+I
Sbjct: 262 SDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMI 321

Query: 312 GDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSAD 371
           GDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAG  K QAVA+R+  D
Sbjct: 322 GDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGD 381

Query: 372 LSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQK 431
           LS FY CSFE YQDTLY HSLRQFYRECDVYGTVDFIFGNAA V Q CNLY R+P   Q 
Sbjct: 382 LSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQS 441

Query: 432 NAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIG 491
           N  TAQGRTDPNQ+TG +IH CTI  A + +   N T   YLGRPWK +SRTV MQ+YI 
Sbjct: 442 NEVTAQGRTDPNQNTGTAIHGCTIRPADD-LATSNYTVKTYLGRPWKEYSRTVVMQTYID 501

Query: 492 DLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFT 551
             + P GW  W+G   L TLYY E+ N GPG++T+ RV W G+ ++N T+A NFTV NF 
Sbjct: 502 GFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFL 559

Query: 552 MGDTWLPYTDIPFSGGLL 564
           +G+ W+  T +PF GGL+
Sbjct: 562 VGEGWIGQTGVPFVGGLI 559

[Close]

BLAST of CmaCh04G014580 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540
           QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFSGGLL 564
           VYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

[Close]

BLAST of CmaCh04G014580 vs. ExPASy TrEMBL
Match: A0A6J1GYC3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1)

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 538/566 (95.05%), Postives = 545/566 (96.29%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL  LSL LAGASDS PPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSAPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V+KCLKQAIKMSSVIGEYL+RGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLNRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKR QKGD EGGNFPAAAQSREPLNTLIKGLH M SCNQSTKC GRKR+LTDLGT
Sbjct: 181 LSRRWKKRRQKGDGEGGNFPAAAQSREPLNTLIKGLH-MSSCNQSTKCWGRKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSM QDGYFVIYVRQGYYDEYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMLQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTT---NYLGRPWKPFSRT 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP WVMDLN TTT   NYLGRPWKPFSRT
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDLNATTTTTMNYLGRPWKPFSRT 480

Query: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAM 540
           VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYG GANTS RV+W GHSLMNITEAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWGGHSLMNITEAM 540

Query: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 564
           NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 565

[Close]

BLAST of CmaCh04G014580 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 8.2e-289
Identity = 491/564 (87.06%), Postives = 525/564 (93.09%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL  AGAS SPP S  LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V++C+KQAIKMS+VIG+YLDRG DSRLNR+E GAL DCRELSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ER+ES LSAIVTNGQTC+DGLVESRSSLG ALSAPLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSC-NQSTKCSGRKRMLTDLG 240
           +SRRWKKR +KG   GG  P   +SRE LNTLIKGLH M  C N STKCSGRKR+LTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300
           ++GILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR+GYY+EYVVVPK+K
Sbjct: 241 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+WVMD N TTTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGPGANTSLRV+W G SLMN+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 564
           TVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

[Close]

BLAST of CmaCh04G014580 vs. ExPASy TrEMBL
Match: A0A0A0KUN8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.3e-288
Identity = 488/564 (86.52%), Postives = 526/564 (93.26%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL LLSL  AGAS SPPPS  LCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V++C+KQA KMS+VIG+YL+RG DSRLNR E GAL DCR+LSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ER+ESILSAIVTNGQTCIDGLVESRSSLGNALS PLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSC-NQSTKCSGRKRMLTDLG 240
           +SRRWKKR +KG   GG  P   +SREPL+TLIKGLH M  C NQSTKC GR+R+LTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300
           +TGILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYV++GYY+EYVVVPK+K
Sbjct: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+WVMD N TTTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGPGANTSLRV+W G SL+N+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSGGLL 564
           TVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

[Close]

BLAST of CmaCh04G014580 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.2e-285
Identity = 483/563 (85.79%), Postives = 522/563 (92.72%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS P+IFL LLSLRLAGASDSPPPSA LCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V++CLKQAIKMSSVIG YL RGG SRLN++E GAL+DCREL+D NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDE+++ER+ESILSAIVTNGQTCIDGLVES+SSLG+ALSAPL +AGELYSVSLGLVKNS
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKR Q       +FP    +REPL TLIKGL  MGSCNQST CSGR+R+LTDLGT
Sbjct: 181 LSRRWKKRRQ-------SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+F+PNNSMP+DGYFVIYVR+GYY+EYVVVPKYKT
Sbjct: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECD+YGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP+W MD N T  NYLGRPWK +SRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540
           QSYIGDLISPVGWLEWNGT GL+TLYYGEFEN+GPGANTS+RV+W G+SLMN T+AMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFSGGLL 564
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFNGGLV 556

[Close]

BLAST of CmaCh04G014580 vs. NCBI nr
Match: XP_022976564.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540
           QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFSGGLL 564
           VYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

[Close]

BLAST of CmaCh04G014580 vs. NCBI nr
Match: XP_023549932.1 (probable pectinesterase/pectinesterase inhibitor 25 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 536/566 (94.70%), Postives = 547/566 (96.64%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL  LSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSIPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V+KC+KQAIKMSSVIGEYL+RGGDSRLNRQE GALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCIKQAIKMSSVIGEYLNRGGDSRLNRQEFGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ERIESILSAIVTNGQTCIDGLVESRSSLGNAL+APLLSAG+LYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKR QKGD EGGNFPAAAQSREPLNTLIKGLH+MGSCNQSTKCSGRKR+LTDLGT
Sbjct: 181 LSRRWKKRKQKGDGEGGNFPAAAQSREPLNTLIKGLHTMGSCNQSTKCSGRKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTT---NYLGRPWKPFSRT 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP WVMD N TTT   NYLGRPWKPFSRT
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDSNATTTTTMNYLGRPWKPFSRT 480

Query: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAM 540
           VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYG GANT  RV+W GHSLMNITEAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTRSRVNWGGHSLMNITEAM 540

Query: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 564
           NFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 566

[Close]

BLAST of CmaCh04G014580 vs. NCBI nr
Match: KAG7032035.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 537/563 (95.38%), Postives = 548/563 (97.34%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL  LSL LAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V+KCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ERIESILSAIVTNGQTCIDGLVESRSSLGNAL+APLLSAG+LYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKR QKGD +GG FPAAAQSREPLNTLIK LH+MGSCNQSTKCS RKR+LTDLGT
Sbjct: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP+WVMDLNE TTNYLGRPWKPFSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNE-TTNYLGRPWKPFSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540
           QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYG GANTS RV+W GHSLMNITEAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFSGGLL 564
           VYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 VYNFTMGDTWLPYTDIPFSRGLL 562

[Close]

BLAST of CmaCh04G014580 vs. NCBI nr
Match: KAG6601245.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 537/566 (94.88%), Postives = 546/566 (96.47%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL  LSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V+KCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ERIESILSAIVTNGQTCIDGLVESRSSLGNAL+APLLSAG+LYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240
           LSRRWKKR QKG  EGG FPAAAQSREPLNTLIKGLH+MGSCNQSTKCSGRKR+LTDLGT
Sbjct: 181 LSRRWKKRKQKGGGEGGKFPAAAQSREPLNTLIKGLHTMGSCNQSTKCSGRKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLN---ETTTNYLGRPWKPFSRT 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP WVMDLN    TT NYLGRPWKPFSRT
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDLNATKTTTMNYLGRPWKPFSRT 480

Query: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAM 540
           VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYG GANTS RV+W GHSLMNITEAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAM 540

Query: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 564
           NFTVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 NFTVYNFTMGDTWLPYTDIPFSRGLL 566

[Close]

BLAST of CmaCh04G014580 vs. NCBI nr
Match: XP_023549916.1 (probable pectinesterase/pectinesterase inhibitor 25 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 536/571 (93.87%), Postives = 547/571 (95.80%), Query Frame = 0


Query: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL  LSL LAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSIPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V+KC+KQAIKMSSVIGEYL+RGGDSRLNRQE GALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCIKQAIKMSSVIGEYLNRGGDSRLNRQEFGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180
           GVDEEL+ERIESILSAIVTNGQTCIDGLVESRSSLGNAL+APLLSAG+LYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIK-----GLHSMGSCNQSTKCSGRKRML 240
           LSRRWKKR QKGD EGGNFPAAAQSREPLNTLIK     GLH+MGSCNQSTKCSGRKR+L
Sbjct: 181 LSRRWKKRKQKGDGEGGNFPAAAQSREPLNTLIKSLNEQGLHTMGSCNQSTKCSGRKRLL 240

Query: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVV 300
           TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV+V
Sbjct: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV 300

Query: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360
           PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH
Sbjct: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360

Query: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420
           QAVAVRNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECDVYGTVDFIFGNAAAVFQQCNL
Sbjct: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420

Query: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTT---NYLGRPWK 480
           YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP WVMD N TTT   NYLGRPWK
Sbjct: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDSNATTTTTMNYLGRPWK 480

Query: 481 PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMN 540
           PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYG GANT  RV+W GHSLMN
Sbjct: 481 PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTRSRVNWGGHSLMN 540

Query: 541 ITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           ITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 ITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 571

[Close]

BLAST of CmaCh04G014580 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 671.0 bits (1730), Expect = 8.3e-193
Identity = 329/550 (59.82%), Postives = 420/550 (76.36%), Query Frame = 0


Query: 20  SDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYL 79
           S S  PS + CKST YPKLCR+IL++++ SPSDPY YGKF++++CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAI 139
            R          +E+GA+ DC ELS+ +V +L+++   L+ A+ +   L+E + S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGDREG 199
           VTN QTC+DGLVE++S    A+ +P+ +   LYS+SLGLV ++L+R  K+ ++ KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTT---GILINNTVVVSS 259
           G     +  REPL TLIKGL    +C+    C    R L +LG T    IL++  V+V  
Sbjct: 254 G---GNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 260 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTV 319
             +DNFT+I DAI+ APNN+ P+DGYFVIY R+G Y+EY+VVP  K N+ML+GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 320 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 379
           ITGNHNVVDGWTTYN S+F V G+ F+A+D+TFRNTAG EKHQAVA+RN+A+ S+FYRCS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 380 FEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 439
           FEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 440 TDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGW 499
            DPNQ+TGISI NCTI AAP+   +     T +LGRPWKP+SRTV+MQSYI D++ PVGW
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAMT-FLGRPWKPYSRTVFMQSYISDIVQPVGW 553

Query: 500 LEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPY 559
           LEWNGT+GLDT+YYGE+ N+GPGANT+ RV W G++L+N+ EAMNFTVYNFTMGDTWLP 
Sbjct: 554 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 613

Query: 560 TDIPFSGGLL 564
           TDIPF GGLL
Sbjct: 614 TDIPFYGGLL 616

[Close]

BLAST of CmaCh04G014580 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 666.4 bits (1718), Expect = 2.0e-191
Identity = 331/549 (60.29%), Postives = 413/549 (75.23%), Query Frame = 0


Query: 22  SPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKMSSVIGEYLDR 81
           SP P  + CKST YPKLCR+ILS+++ SPSDPY YGKF++++CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIESILSAIVT 141
                     +EV A+ DC EL++ +VE+L+++   L+AAE +   L++R+ S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKK-RSQKGD-REGG 201
           N QTC+DGLV+++S    A+  PL +   LYSVSLGLV ++L+R  K+ +  KG    GG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 NFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTT---GILINNTVVVSST 261
           N P     REPL TLIK L    +C++   C    R L +LG T    IL+   V V   
Sbjct: 260 NKPV----REPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 262 GADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVI 321
             DNF +I +A++ APN++ P+ GYFVIY R G Y+EYVV+   K NIMLIGDGIN+T+I
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 322 TGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSF 381
           +GNH+ +DGWTTYNSSTF V GD FVA+D+TFRNTAG EKHQAVAVRN+AD STFYRCSF
Sbjct: 380 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 439

Query: 382 EGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRT 441
           EGYQDTLYVHSLRQFYRECD+YGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRT
Sbjct: 440 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 499

Query: 442 DPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 501
           DPNQ TGISI NCTI AAP+   D   T T +LGRPWKP+SRTVY+QSYI D++ PVGWL
Sbjct: 500 DPNQKTGISIINCTIGAAPDLAADPKSTMT-FLGRPWKPYSRTVYIQSYISDVVQPVGWL 559

Query: 502 EWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYT 561
           EWNGT GLDT+ YGE++N+GPGA+TS RV W G+SL+N+ +AMNFTVYNFT+GDTWLP T
Sbjct: 560 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQT 619

Query: 562 DIPFSGGLL 564
           DIPF GGLL
Sbjct: 620 DIPFYGGLL 621

[Close]

BLAST of CmaCh04G014580 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 502.3 bits (1292), Expect = 5.1e-142
Identity = 273/570 (47.89%), Postives = 367/570 (64.39%), Query Frame = 0


Query: 7   IFLFLLSLRLAGASDSPP-PSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCL 66
           +FL L +L +A  +  P   S+ +CK+T  PK C+S+    + S  D   YG+FS+RK L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQS---IDGVLEAAEGV 126
            Q+ K +  I  YL R  ++ L++  VGAL+DCR L+    ++L +      +  +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEELMERIESILSAIVTNGQTCIDGLVESRSS---LGNALSAPLLSAGELYSVSLGLVKN 186
                + I+++LSA +TN QTC+DG+  + SS   + N ++ PL++  +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLAL--- 190

Query: 187 SLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQS----TKCSGRKR 246
             ++ W  KK+ Q       +        +P      G   +     +       S RK 
Sbjct: 191 -FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL 250

Query: 247 MLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV 306
              D     +L+++ V V+  G  NFT+I +A++ APN +    GYFVIYV  G Y+E V
Sbjct: 251 ADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENV 310

Query: 307 VVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAE 366
           V+ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA++MTFRNTAG E
Sbjct: 311 VIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPE 370

Query: 367 KHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQC 426
           KHQAVA+R+SADLS FY CSFE YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ C
Sbjct: 371 KHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDC 430

Query: 427 NLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKP 486
           NLY R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTI  A + V   N T   YLGRPWK 
Sbjct: 431 NLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSS-NYTVKTYLGRPWKE 490

Query: 487 FSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNI 546
           +SRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV W G+ ++N 
Sbjct: 491 YSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINS 550

Query: 547 TEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           T+A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 TDANNFTVENFLLGDGWMVQSGVPYISGLL 572

[Close]

BLAST of CmaCh04G014580 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 464.9 bits (1195), Expect = 9.0e-131
Identity = 265/587 (45.14%), Postives = 361/587 (61.50%), Query Frame = 0


Query: 4   APFIFLFLLSLRL---AGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 63
           +P   L  LS  L     +S +   S+ +CK+T  PK C+S+      S  +   YG FS
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFS 62

Query: 64  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEV-GALEDCRELSDSNVEF-LQSIDGVLEA 123
           +RK L Q+ K    +  Y+ R  ++ L++  V  AL+DCR L+   +++ L S + V + 
Sbjct: 63  IRKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDT 122

Query: 124 AEGVDEELM-----ERIESILSAIVTNGQTCIDGLVESRS-----SLGNALSAPLLSAGE 183
           +     + +     + I+++LSA +TN QTC++GL  + S     ++   ++ PL++  +
Sbjct: 123 SAKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTK 182

Query: 184 LYSVSLGLVKNSLSRRW--KKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQST 243
           L  VSL L     ++ W  KK+ + G         ++   +P      G   +    + T
Sbjct: 183 LLGVSLAL----FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPL-KMTEKT 242

Query: 244 KC---SGRKRMLTDLGTTG-------ILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQ 303
           K    S  +R L D  + G       +LI++ V VS  G  NFT+I  A++ APNN+   
Sbjct: 243 KAVYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGS 302

Query: 304 DGYFVIYVRQGYYDEYVVVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYG 363
            G+F+IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V  
Sbjct: 303 AGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTA 362

Query: 364 DGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVY 423
             FVA+++TFRNTAG EKHQAVA+R+ AD S FY CSFE YQDTLY HSLRQFYRECDVY
Sbjct: 363 PNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVY 422

Query: 424 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWV 483
           GTVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTI  A + V
Sbjct: 423 GTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV 482

Query: 484 MDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPG 543
              N T   YLGRPWK +SRTVYMQSYI   + PVGW EWNG   L TLYY E+ N GPG
Sbjct: 483 SS-NYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPG 542

Query: 544 ANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 564
           +NT+ RV W G+ ++N T+A NFTV    +   W+  T +P++ GL+
Sbjct: 543 SNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

[Close]

BLAST of CmaCh04G014580 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 463.8 bits (1192), Expect = 2.0e-130
Identity = 252/558 (45.16%), Postives = 337/558 (60.39%), Query Frame = 0


Query: 12  LSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVRKCLKQAIKM 71
           +S ++    ++  PS + C+    P  CRS+L +    P D Y YG+ S+R+ L +A + 
Sbjct: 22  ISAQIPAIGNATSPSNI-CRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRF 81

Query: 72  SSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELMERIE 131
            S+I   LDR G     +  VGALEDC+ L+   +++L S     ++ + +     E + 
Sbjct: 82  ISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVH 141

Query: 132 SILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNSLSRRWKKRSQK 191
           + LSA +TN QTC++GL  + S   N LS  L +  +LY VSL L     S+ W  R Q+
Sbjct: 142 TFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQR 201

Query: 192 G------DREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGTTGILI 251
                        F      + PL         M    ++   +  +R L       + +
Sbjct: 202 SRPIWQPQARFKKFFGFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQV 261

Query: 252 NNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKTNIMLI 311
           ++ V V   G  NFT+I  AI+ APN +   +GYF+IYV  G Y+EYV VPK K  +M+I
Sbjct: 262 SDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMI 321

Query: 312 GDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSAD 371
           GDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAG  K QAVA+R+  D
Sbjct: 322 GDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGD 381

Query: 372 LSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQK 431
           LS FY CSFE YQDTLY HSLRQFYRECDVYGTVDFIFGNAA V Q CNLY R+P   Q 
Sbjct: 382 LSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQS 441

Query: 432 NAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYMQSYIG 491
           N  TAQGRTDPNQ+TG +IH CTI  A + +   N T   YLGRPWK +SRTV MQ+YI 
Sbjct: 442 NEVTAQGRTDPNQNTGTAIHGCTIRPADD-LATSNYTVKTYLGRPWKEYSRTVVMQTYID 501

Query: 492 DLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFTVYNFT 551
             + P GW  W+G   L TLYY E+ N GPG++T+ RV W G+ ++N T+A NFTV NF 
Sbjct: 502 GFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFL 559

Query: 552 MGDTWLPYTDIPFSGGLL 564
           +G+ W+  T +PF GGL+
Sbjct: 562 VGEGWIGQTGVPFVGGLI 559

The following BLAST results are available for this feature:

BLAST of CmaCh04G014580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94CB1	1.2e-191	59.82	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF77	2.9e-190	60.29	Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3...	[more]
Q8RXK7	7.2e-141	47.89	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	1.3e-129	45.14	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O22256	2.8e-129	45.16	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmaCh04G014580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1INW0	0.0e+00	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1	[more]
A0A6J1GYC3	0.0e+00	95.05	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1	[more]
A0A1S3BFR9	8.2e-289	87.06	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1	[more]
A0A0A0KUN8	5.3e-288	86.52	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1	[more]
A0A6J1D8L4	4.2e-285	85.79	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1	[more]

BLAST of CmaCh04G014580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022976564.1	0.0e+00	100.00	probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima]	[more]
XP_023549932.1	0.0e+00	94.70	probable pectinesterase/pectinesterase inhibitor 25 isoform X3 [Cucurbita pepo s...	[more]
KAG7032035.1	0.0e+00	95.38	putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosp...	[more]
KAG6601245.1	0.0e+00	94.88	putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosp...	[more]
XP_023549916.1	0.0e+00	93.87	probable pectinesterase/pectinesterase inhibitor 25 isoform X1 [Cucurbita pepo s...	[more]

BLAST of CmaCh04G014580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10720.2	8.3e-193	59.82	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	2.0e-191	60.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	5.1e-142	47.89	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	9.0e-131	45.14	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	2.0e-130	45.16	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 20..176 e-value: 1.1E-20 score: 84.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..173 e-value: 1.2E-16 score: 61.4
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 249..548 e-value: 1.1E-134 score: 447.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..180 e-value: 1.0E-22 score: 82.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..179
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 243..562 e-value: 1.2E-114 score: 385.0
None	No IPR available	PANTHER	PTHR31707:SF231	PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATED	coord: 5..562
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 5..562
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 29..179 e-value: 6.85569E-28 score: 107.524
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 395..404
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 244..561

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G014580.1	CmaCh04G014580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity