CmaCh04G010690 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G010690
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	Cma_Chr04: 5514792 .. 5520319 (-)
RNA-Seq Expression	CmaCh04G010690
Synteny	CmaCh04G010690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACGACTTCCCGCCATGTTAACTGTGATGCTGAATACCCTATCTCCTTCCCCATTTCTTCGCAGCCAACAAACAATTTCCCATTGCTTCTCCTCTTCAACGATTCATCTCTTCCAATTTTCACAGTCGCAGAAAAAACCCCTCAACAATTGCCTCCCAAACAATCGCACCGAAAGGGGCATAGAATTCGACATTGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCGGCGGCGCTTTACCGTAAATCCAAGGGAAGTCTTCGGGTTCTCGACGCATTGTGCGGTTGCGGGATTCGGTCGCTTCGGTACTTGGTCGAGGCTGAGGCTGATTTTGTGTTGGCGAATGACGCCAATGACGAAAATCGGGGGGTTATAGTAAAGAATCTATCGAGGGTTGCGGAAGGTTTTGGGGAGACTCGGCGGTGGATGGTTACCAATTGTGATGCGAACAGGGTTATGTCGGAGTTCTATTTGCAAAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTTTTGAGGCCAGCTATTAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGCGGCCATCACCCTTCTCAGTGAGTGCACAATCTTATCTGTTACAATTTGTATTCCATTACTTGTTTGCATTAACCGCTTCATTGAAAATCTTGATATGAAATGTAACCGCTAAAATCTTGATCAATGTAAGGGCTTTTATCTATATATAACTGATTGAATATATAACATTATTTTAGTATGAAGTTTTAGTAGTTCCATATGGTATTACCTCTCAATTAATATTTCTATATCTCTTATTGAGTCTTAAAGCCGACGTATTGGTTTAAGAAAATTTTAAAACTGAAGATAGAGCCGAATTTTTAAAAAGCTTAACTGACAAAACCAGCCAAAATTGAAAAGGCATACCAAGACTAACAGAATTGATCCTAACCATTCCGAATGTTGTTTTTTTTGGTTTTGACTTGTACCCTTGTGCTAAGTTTTAAGTCTACAAGCGAAATGAGTGCTAAGTAGTAGTTTGAATATTGGAGGGTAATTATATGAATGTGTATGCTATACTATGGCTGGTGTGGTAATTAAAAATTCCTTTTTCCTTTCTCTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCTCTTTCTAGATCTTTAGCTGCATATGGAGCATATGTACGTCCCATGCCATTTTCAAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGTAACGCCACTCTTTTCGTATTATTCATACCATGGACCTGTTTTTAGAGTTATGCTTCGAATCAGTCGAGGGAGGATCAATAAAAACAGGTGATAGTTGACGAGTTTAGAATGTCTGCTAGTGCTGGATGTATCTTGTATGATAATTGATGATGCTTGATTCATGCTCCCACTATTTATGTCAGGCATTATGGTTTCGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGGTTTTTCATGGAGTGAGGTTGGACAGATGAGTTGTCCTTGCAATGATACAAGAGTAAGACTGACTATGACTGGCATTCTGCTACTCTTTTGAGCTTAAGCATCCACTGGTTTCAGCAGCTTCCATATTTTAAACTGATACCGAAAGTTCCTCATATAATTTGCATGGTCCATATAGCCATGGCTTCAAGTTGAATATGCTTGAATTTGTTGTTTGCTTATTCGTTGGGTGCTTTCTTTTTAATGTAAATACTTGCAACATTTAATCTTGTAGTTAAAAAAATTTCCATGTTAGGTTTCCGAGTCACTTGTGGTCTCGGGACCTCTTTGGACTGGGCCTCTACACAGTGCAGACTACATTGAAGACATGTTAACCTTAGCTATGCAGTGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGGGGCAAATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTATATCAAAATGGACGAGGTGTTTATTTTCTCATGGTTTAATATTTCATTTCTGTTTCTGGACCAACAATGTAAGACGTGATGTATCAACCTCCCATGCTTAGGTGGCAAGTCGGGCAAAAGTAAATTCGCCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGTGAGAGTATAGGTAGTTAAGATTTGATAAATTTTGTGATGCATGTTATTGCCCACGCGGCTAAACTGTTTTTGTTGTCATGAGATTCTACTACATTCTGGAAATCAAAGAAATGTTGGCATAGACATGACGGGTTATTTTCTATGAGTATAGGTCATAAATGTGGCCTGCATACGTTTACTTAAATTTATATATTTTAAGACTATCATTTTTATACAAGACAATTTAAAGTCTGTATGTTGTGGACAGTTTCCCAAACTAGAGTGTTTGCGTTTCATAACTAGAGAACAGGATTTTGCGCGGAATGTAGATGCGTGAAGAGAAAAAAAGTTCTCTCTTTTCTTTTTATCTCTCTCTGAGGATTTTTGGTGTTGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACGAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCTTGAACTGCAGCAGAGTTGTCTTGGTGTGCATCAAGTCACATGAATATTGTAGCGAGTGCTTGCAGCTATGGAAAGTACAGCACTTTCACAGGAAAACTAGAGCGAAACCAAAGCGGTGTAAGCATCCATCTTTCTCTATTCTCAGTTTGAAAATTGATGAAGATAATTATGGGTCACATGCATTAACCGTGGAAATGTGCTCAATAAACATGTGAATTATTTAGAAAGAAAAAGTTAAGTTACAGTCTTTTATACTTCATATTTGCATTTTGTCAGCTTTAAAGACATTTAAAGTACTTTCAGTCTGTTGTTTCCTCACAGATCTTTCATTTTGTTCTTATTATGCGCTCGTTTATATTATGCCTGTATCTTTCTGCATGAGCATGAATTTTTTTCCACAATTACTTGAGTTCCCTTTTGCAAATTTGATGACTACTTTTAAATTTGGCAAATGTTGTAATATTTTACATTATGTTAGGTATTTTCCTGTGAAGCATTATTAAAAATAATGTTCTTACCCGATATCATTGAATTACAAACAAATGCTGTGGAAAAAAATTGACAGGGCTTTAGATTAAGATGCTGCTGAGGGATGGTTTTTGGGTAAAATTGGTAAGTATTTTGGAAAATTTATTACATTGATCTGTAGAACTTAGTACGTTAATTTGTAGAACATTTGAACGAAAGCTTAATAGTATAAATTTTCTGTTGATACTAAGAAAGAGATTCAATAGTATAACTGATAGGTTCTAGATAGGAACACCCTTCAATAATACTTCACAACATATTTTATTATCCCTCAGAAGTCACTTATAAAGAGAGACTAGTTGATTTAAAGGACTCATTTTGGATACGAATCTCTCGACTTTTGATTACCTTTTACATAAAGGAATAGAGATCGAGAAACTAGATACCCTTTTTATTTAAGTAATGACATAATCTAGCTCTTTCACAACTTCCTAAATTTCTTGGCCTTCCCGTCTCTGAGCATTGTCTAAGATTCAGAATGGAACAAGGGGATATGCGTCATGATATACATCTTGCAGTCATGAGAGTCAACTTCAGCCCCAAATGAATCACGGCTAATGCTATCTAGAAATTTGTGCTGCAAGTATTGGTGATGAGACGACCAAGTTAGATTAAACAACTCATACTGCATTGCATCAAGATAGACTAAGGAATGAAGCACATACAGACACAAGCATTGTCATGTCATTTAAACTAAAGTGTTGTTGCTTCATAGAAGTCGTTAAATAAGATGAGAATATCAGTTCATGCATACTGAGTAAATTAGTTTAGATGAGCTAGGATCCTGACCTCCCATAAGTCCCTTACTGAGACAATGACGTCGAATATAACTCCAAGTTCAAGCCAGCTTACTGAGATCATTGAAGCAGTTGAACATCGATTCCAAAGAACAATAGCCAAGCGAATCTCAGGTGCAGAGCTTGATAAGAGACCTGCCCACACCTGTAAGTCACAACTCTTTCTTTTTACTTTGCTTCTCATGGAGCCATTGTAAGTCATGCTGTTCTCTTCTTATGTGCCGAAATAAAATATTAATCTAGCAAAGTGTAGGCGATATAAAGGTACTTATAGTCCTTAAACTTCAAATTTTATGTCAAAAGTGTTTGAAATTTTTAATTTTGTGTGTAATAAAATATCTCTGTCGTTAATTCTGTCAGTTTAACTCTCATGCACGCTGACTAGATTTATAAGGACGGTGGGGTAAATTGGTTTTAAATGGGTTTAGGTTTTTCTTGGTGTTTCTTGTGTAGGTTTAGTTTGTTTTTGCTGGTTTGCATGCCTCACTCATTGACTCGTTCACCAAACACCTAACCCCACACCTAAGAAACACCAGAAAGTTTAAGGATCTAATAAAAACTGTAAACTAAATAAGACGAGTTTGAAGTTTAAATTCATAACTTTGTTATGCTAATACAATGTATAGATGAAGCAAAGAGTTAAAAAGAGAACCTGAAGACAACCATCTTTGCCAGAAACATTAATTTTTCTTCCCTGGAGCCCAAGACTATCTGCGTCCATAATTTAAGTTTCCCTTTTGATATGTGAGCAAATAATAAGTGTTCTTGTTTAAAAGGGGGTATAAGATAAGAAAAGATAGTATAAATAAATTTTGATCACCTTGGTTTACATCAATGACTTCCTTATTGCTTAGAATTTCCAAAGTTTGTGGAGACATGTTTCTCACATCACAACCAATCAACAGAGGGGCCTGCATGCACATTTGGATGAACTCAAAACGAGCACAAAATGAGCATGTTTGATAACCATTTGGGGTTTTATTTTTAGAAAGTAAATCTTATTAGGCTGCTTTTATCTACAAGTTATTTGTTTGTTAAAATTTTCAAATTCTATGGTTATTGTAAAAATGAAACCTTCATTAAAGCCCATATGCTAAAATGGCTTCTGTATTCTTGATAGGTCATGCCACCATTGCCAACTTCCAACATATCTGGATCTGTCCACACACACACATGGTAAGATGGGAATTACAGATCAACTTAGAGAACAAATGGATGAAGTTGAATGGGTTTCAAACACAATCAATCTCATACCATTCCAACCACCAGGTCCTGCATACTCTGCCCACTTATCATTGATATCTGCAATTCTAGTCATGCTTCAACCAAAAAAAAAACAATTTTTTTGAAGTTAATTAAATGCAACATTTTAGGGGTTATTTAGTACAATAATGGGAAGATTTTAGGGCTAGATTTAAACCTAACCTTTCCCATGAATCACTTATATCAATTGTTGTACGCCAACTGTTTCCAACTCTAGGAGCCCATAGTGCTGGATCATCAACGCCCCTAACCAC

mRNA sequence

AACGACTTCCCGCCATGTTAACTGTGATGCTGAATACCCTATCTCCTTCCCCATTTCTTCGCAGCCAACAAACAATTTCCCATTGCTTCTCCTCTTCAACGATTCATCTCTTCCAATTTTCACAGTCGCAGAAAAAACCCCTCAACAATTGCCTCCCAAACAATCGCACCGAAAGGGGCATAGAATTCGACATTGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCGGCGGCGCTTTACCGTAAATCCAAGGGAAGTCTTCGGGTTCTCGACGCATTGTGCGGTTGCGGGATTCGGTCGCTTCGGTACTTGGTCGAGGCTGAGGCTGATTTTGTGTTGGCGAATGACGCCAATGACGAAAATCGGGGGGTTATAGTAAAGAATCTATCGAGGGTTGCGGAAGGTTTTGGGGAGACTCGGCGGTGGATGGTTACCAATTGTGATGCGAACAGGGTTATGTCGGAGTTCTATTTGCAAAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTTTTGAGGCCAGCTATTAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGCGGCCATCACCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTACGTCCCATGCCATTTTCAAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGTAACGCCACTCTTTTCGTATTATTCATACCATGGACCTGTTTTTAGAGTTATGCTTCGAATCAGTCGAGGGAGGATCAATAAAAACAGGCATTATGGTTTCGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGGTTTTTCATGGAGTGAGGTTGGACAGATGAGTTGTCCTTGCAATGATACAAGAGTTTCCGAGTCACTTGTGGTCTCGGGACCTCTTTGGACTGGGCCTCTACACAGTGCAGACTACATTGAAGACATGTTAACCTTAGCTATGCAGTGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGGGGCAAATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTATATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAATTCGCCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACGAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCTTGAACTGCAGCAGAGTTGTCTTGGTGTGCATCAAGTCACATGAATATTGTAGCGAGTGCTTGCAGCTATGGAAAGTACAGCACTTTCACAGGAAAACTAGAGCGAAACCAAAGCGGTGTAAGCATCCATCTTTCTCTATTCTCAGTTTGAAAATTGATGAAGATAATTATGGGTCACATGCATTAACCGTGGAAATGTGCTCAATAAACATGTGAATTATTTAGAAAGAAAAAGTTAAGTTACAGTCTTTTATACTTCATATTTGCATTTTGTCAGCTTTAAAGACATTTAAAGTACTTTCAGTCTGTTGTTTCCTCACAGATCTTTCATTTTGTTCTTATTATGCGCTCGTTTATATTATGCCTGTATCTTTCTGCATGAGCATGAATTTTTTTCCACAATTACTTGAGTTCCCTTTTGCAAATTTGATGACTACTTTTAAATTTGGCAAATGTTGTAATATTTTACATTATGTTAGGTATTTTCCTGTGAAGCATTATTAAAAATAATGTTCTTACCCGATATCATTGAATTACAAACAAATGCTGTGGAAAAAAATTGACAGGGCTTTAGATTAAGATGCTGCTGAGGGATGGTTTTTGGGTAAAATTGGTAAGTATTTTGGAAAATTTATTACATTGATCTGTAGAACTTAGTACGTTAATTTGTAGAACATTTGAACGAAAGCTTAATAGTATAAATTTTCTGTTGATACTAAGAAAGAGATTCAATAGTATAACTGATAGGTTCTAGATAGGAACACCCTTCAATAATACTTCACAACATATTTTATTATCCCTCAGAAGTCACTTATAAAGAGAGACTAGTTGATTTAAAGGACTCATTTTGGATACGAATCTCTCGACTTTTGATTACCTTTTACATAAAGGAATAGAGATCGAGAAACTAGATACCCTTTTTATTTAAGTAATGACATAATCTAGCTCTTTCACAACTTCCTAAATTTCTTGGCCTTCCCGTCTCTGAGCATTGTCTAAGATTCAGAATGGAACAAGGGGATATGCGTCATGATATACATCTTGCAGTCATGAGAGTCAACTTCAGCCCCAAATGAATCACGGCTAATGCTATCTAGAAATTTGTGCTGCAAGTATTGGTGATGAGACGACCAAGTTAGATTAAACAACTCATACTGCATTGCATCAAGATAGACTAAGGAATGAAGCACATACAGACACAAGCATTGTCATGTCATTTAAACTAAAGTGTTGTTGCTTCATAGAAGTCGTTAAATAAGATGAGAATATCAGTTCATGCATACTGAGTAAATTAGTTTAGATGAGCTAGGATCCTGACCTCCCATAAGTCCCTTACTGAGACAATGACGTCGAATATAACTCCAAGTTCAAGCCAGCTTACTGAGATCATTGAAGCAGTTGAACATCGATTCCAAAGAACAATAGCCAAGCGAATCTCAGGTGCAGAGCTTGATAAGAGACCTGCCCACACCTGTCATGCCACCATTGCCAACTTCCAACATATCTGGATCTGTCCACACACACACATGGTAAGATGGGAATTACAGATCAACTTAGAGAACAAATGGATGAAGTTGAATGGGTTTCAAACACAATCAATCTCATACCATTCCAACCACCAGGTCCTGCATACTCTGCCCACTTATCATTGATATCTGCAATTCTAGTCATGCTTCAACCAAAAAAAAAACAATTTTTTTGAAGTTAATTAAATGCAACATTTTAGGGGTTATTTAGTACAATAATGGGAAGATTTTAGGGCTAGATTTAAACCTAACCTTTCCCATGAATCACTTATATCAATTGTTGTACGCCAACTGTTTCCAACTCTAGGAGCCCATAGTGCTGGATCATCAACGCCCCTAACCAC

Coding sequence (CDS)

ATGTTAACTGTGATGCTGAATACCCTATCTCCTTCCCCATTTCTTCGCAGCCAACAAACAATTTCCCATTGCTTCTCCTCTTCAACGATTCATCTCTTCCAATTTTCACAGTCGCAGAAAAAACCCCTCAACAATTGCCTCCCAAACAATCGCACCGAAAGGGGCATAGAATTCGACATTGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCGGCGGCGCTTTACCGTAAATCCAAGGGAAGTCTTCGGGTTCTCGACGCATTGTGCGGTTGCGGGATTCGGTCGCTTCGGTACTTGGTCGAGGCTGAGGCTGATTTTGTGTTGGCGAATGACGCCAATGACGAAAATCGGGGGGTTATAGTAAAGAATCTATCGAGGGTTGCGGAAGGTTTTGGGGAGACTCGGCGGTGGATGGTTACCAATTGTGATGCGAACAGGGTTATGTCGGAGTTCTATTTGCAAAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTTTTGAGGCCAGCTATTAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGCGGCCATCACCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTACGTCCCATGCCATTTTCAAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGTAACGCCACTCTTTTCGTATTATTCATACCATGGACCTGTTTTTAGAGTTATGCTTCGAATCAGTCGAGGGAGGATCAATAAAAACAGGCATTATGGTTTCGTCAGCTATTGCAAAAAATGTGGAAATTCACAAGGTTTTTCATGGAGTGAGGTTGGACAGATGAGTTGTCCTTGCAATGATACAAGAGTTTCCGAGTCACTTGTGGTCTCGGGACCTCTTTGGACTGGGCCTCTACACAGTGCAGACTACATTGAAGACATGTTAACCTTAGCTATGCAGTGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGGGGCAAATGGTGGATGAGAGTGACTCCAAACTACCAGTAGGGTATATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAATTCGCCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACGAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCTTGAACTGCAGCAGAGTTGTCTTGGTGTGCATCAAGTCACATGA

Protein sequence

MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQSCLGVHQVT

Homology

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BLAST of CmaCh04G010690 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.1e-34
Identity = 120/378 (31.75%), Postives = 181/378 (47.88%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    A  RD+ VLA     K+ GS RVLDAL   GIR +RY +E  A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NRGVIVKNLSRVAEGFGE----------TRRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 183
              +IV N+     G  E           +  +VTN DANR+M+E   Q  +FDF+D+D 
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNE---QFRYFDFVDLDP 146

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ A+K  G+L +T+TD     G + +  L  Y A        +E GLR+
Sbjct: 147 FGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYRNACLRKYLAEPIRGELCHEAGLRI 206

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQ 303
           LIG + R A+     V  L +YY  H   FR  L++  G    ++    + Y  +     
Sbjct: 207 LIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ----- 266

Query: 304 GFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKD 363
                E G+      +T +       GPLW GPL + +++E++  L+ +   +G+   K 
Sbjct: 267 ----DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS---KK 326

Query: 364 LEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNA 423
            +K LG + DE +  +P  Y     VA R  +    L  ++  + + GY A+R+H +  A
Sbjct: 327 TQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTA 377

Query: 424 IKTNCPMAECIRIALELQ 432
           +KT+ P  E +    ELQ
Sbjct: 387 LKTDAPFEEVLEALRELQ 377

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BLAST of CmaCh04G010690 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 2.8e-31
Identity = 103/369 (27.91%), Postives = 175/369 (47.43%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    A  RD+ V+   + +       VLDAL   GIR +R+ +E  A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPR----IVLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NRGVIVKNL-----SRVAEGFGET-----RRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 183
              ++ KN+       + E  G       +  +V + DANR+M+E   +  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L +T+TDG    G HP   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN---RHYGFVSYCKKCG 303
           L+G VAR A+     +  + +YY  H   FR  +++  G    +    + G++ + +  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQ 363
                   EV +   P             GP+W GPL S + +E+ML ++ Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]

BLAST of CmaCh04G010690 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.6e-31
Identity = 107/369 (29.00%), Postives = 172/369 (46.61%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    A  RD+ VL   + +       VLDAL   GIR +R+ +E  A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPK----IVLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NRGVIVKNLSRVAEG----------FGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDS 183
              ++ KN+    +G          F   +  ++ N DANR+M+E   +  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 184 FGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L VT+TDG    G HP   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN---RHYGFVSYCKKCG 303
           L+G +AR A+     +  L +YY  H   FR  +++  G    +    + G+V + +K G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQ 363
             +                + +S      GPLW GPL +   + +ML L +    + N  
Sbjct: 269 RFE-------------VEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]

BLAST of CmaCh04G010690 vs. ExPASy Swiss-Prot
Match: Q9YDY7 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=trm1 PE=3 SV=3)

HSP 1 Score: 135.6 bits (340), Expect = 1.4e-30
Identity = 110/387 (28.42%), Postives = 187/387 (48.32%), Query Frame = 0


Query: 62  DTFFRHESATGRDLGVLAAALYRKSKGSLR---VLDALCGCGIRSLRYLVEAEADFVLAN 121
           + F+       RD+ V+AA+  R++    R     DA  G G+R +RY VEA    V+ N
Sbjct: 39  EVFYNPAMEFNRDVSVVAASALRRTGLLTRSGVAFDAHAGVGVRGVRYAVEAGYVKVIMN 98

Query: 122 DANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDF---FDFIDVDSFG 181
           D N   +  ++  L+  A G  E   +M+ N ++N +M  F+L ++       ID+D +G
Sbjct: 99  DINP--KASMLAALNARANGL-EPGSYMIFNKESNSLM--FHLSRERPTPVSLIDIDPYG 158

Query: 182 SDSTFLRPAINALKLDGLLYVTSTD-GFSSGGHHPSQSLAAYGAY----VRPMPFSNEIG 241
           S + F+  A+       ++ +T+TD     GG    ++ AA   Y    V   P S E G
Sbjct: 159 SPAPFVDAALALSGKGTVVAMTATDLAVLEGG----KARAAVRRYMLRSVSKTPVSKETG 218

Query: 242 LRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKN----RHYGFVSYC 301
           LR+L+G VAR A+     V PL +YY+ H   +RV + + RG    +     + G + YC
Sbjct: 219 LRVLLGYVARVAAAHDKAVKPLLAYYADH--YYRVYVAVERGARRSDSMLEENLGRLVYC 278

Query: 302 KKCGNSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWI 361
            + G +   S++E    +C          +V + P W G L    ++E ML +A++  W+
Sbjct: 279 PETGVALALSYAEDPASACG------GSYVVAADPAWIGSLGDQAFLEAMLNIAVEAVWL 338

Query: 362 GNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASR 421
           G      +EKLL  +  E+       Y+ +  VAS+A+VN+P  + ++  +   GY A  
Sbjct: 339 GT--RPRVEKLLNTLHGEAPLSPRSIYVSLTSVASKARVNTPKKSKVVELLRSMGYRAVA 398

Query: 422 SHIASNAIKTNCPMAECIRIALELQQS 434
           +H +   ++T+ P  E +   ++L  S
Sbjct: 399 THFSGEGVRTDAPWGEVLEAVVKLGSS 406

[Close]

BLAST of CmaCh04G010690 vs. ExPASy Swiss-Prot
Match: Q5JIB3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=trm1 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 9.0e-30
Identity = 106/367 (28.88%), Postives = 176/367 (47.96%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDANDE 123
           F+    +  RD+ VLA     K  G  RVLDAL   GIR +RY +E  A+ V  ND ++E
Sbjct: 27  FYNPVMSLNRDISVLAV----KVLGPKRVLDALSATGIRGIRYALETPAEEVWLNDISEE 86

Query: 124 NRGVIVKNLSRVAEG-----------FGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVD 183
             G++ KN S   +G           +GE +  ++   DANR+M+E +    +FDF+D+D
Sbjct: 87  AYGLMKKNASLNIDGELYEEGDRSYLWGE-KLVVINKGDANRLMAENF---RYFDFLDLD 146

Query: 184 SFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLR 243
            FGS   FL  A+ +++ +G+L VT+TD     G + +  L  Y A     P  +E GLR
Sbjct: 147 PFGSPVEFLDTALRSVRRNGVLAVTATDTGVLCGAYRNACLRKYLAEPIRGPLCHEAGLR 206

Query: 244 MLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNS 303
           +LIG V R A+     V  L +Y+  H   FR  L++  G               K  + 
Sbjct: 207 ILIGTVVRYAAKYDLGVEVLLAYHRDH--YFRAFLKLKSG----------AKKADKSLSQ 266

Query: 304 QGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGK 363
            GF W++            + +    SGPLW GPL    ++E++L  A     + + +  
Sbjct: 267 LGFLWADKSG-RFEYRRGFLPDKPGASGPLWLGPLKDESFVEELLESARDHP-LAHKKTL 326

Query: 364 DLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASN 420
              +L+ + +D     +P  Y     +A    +  P L+ ++  + + G++A+++H +  
Sbjct: 327 SFLQLISEELD-----VPFHY-DTHTLARACSLTPPKLDVIIQRLRELGHSATKTHFSPT 365

[Close]

BLAST of CmaCh04G010690 vs. ExPASy TrEMBL
Match: A0A6J1JBD3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484232 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.2e-256
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIALELQQSCLGVHQVT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of CmaCh04G010690 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.3e-254
Identity = 437/441 (99.09%), Postives = 439/441 (99.55%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTVMLNTLSPSPFLR+QQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFYVTPLFSYYSYHGPVFRVMLRI+RGRINKNRHYGFVSYCKKCGNSQ FSWSEV
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIALELQQSCLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of CmaCh04G010690 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 6.3e-228
Identity = 395/441 (89.57%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIA +LQ++CLG HQVT
Sbjct: 421 AECIRIA-QLQRNCLGAHQVT 440

[Close]

BLAST of CmaCh04G010690 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.3e-227
Identity = 391/441 (88.66%), Postives = 416/441 (94.33%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           A+CIRIA +LQQ+CLGVHQVT
Sbjct: 421 ADCIRIA-QLQQNCLGVHQVT 440

[Close]

BLAST of CmaCh04G010690 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.3e-227
Identity = 391/441 (88.66%), Postives = 416/441 (94.33%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           A+CIRIA +LQQ+CLGVHQVT
Sbjct: 421 ADCIRIA-QLQQNCLGVHQVT 440

[Close]

BLAST of CmaCh04G010690 vs. NCBI nr
Match: XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])

HSP 1 Score: 893.6 bits (2308), Expect = 6.6e-256
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIALELQQSCLGVHQVT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of CmaCh04G010690 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 887.5 bits (2292), Expect = 4.7e-254
Identity = 437/441 (99.09%), Postives = 439/441 (99.55%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTVMLNTLSPSPFLR+QQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFYVTPLFSYYSYHGPVFRVMLRI+RGRINKNRHYGFVSYCKKCGNSQ FSWSEV
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIALELQQSCLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of CmaCh04G010690 vs. NCBI nr
Match: XP_023529370.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 882.5 bits (2279), Expect = 1.5e-252
Identity = 436/441 (98.87%), Postives = 437/441 (99.09%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQ K LNNCLPNNRTERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQIKSLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENRGVIVKNLS+VAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQ FSWSEV
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
            ECIRIALELQQSCLGVHQVT
Sbjct: 421 TECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of CmaCh04G010690 vs. NCBI nr
Match: KAG6600883.1 (Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818.5 bits (2113), Expect = 2.7e-233
Identity = 401/405 (99.01%), Postives = 402/405 (99.26%), Query Frame = 0


Query: 37  QSQKKPLNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDAL 96
           QSQKKPLNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDAL
Sbjct: 431 QSQKKPLNNCLPNNRTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDAL 490

Query: 97  CGCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVM 156
           CGCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSRVAEGFGE RRWMVTNCDANRVM
Sbjct: 491 CGCGIRSLRYLVEAEADFVLANDANDENRGVIVKNLSRVAEGFGEIRRWMVTNCDANRVM 550

Query: 157 SEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAY 216
           SEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAY
Sbjct: 551 SEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAY 610

Query: 217 GAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINK 276
           GAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRI+RGRINK
Sbjct: 611 GAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINK 670

Query: 277 NRHYGFVSYCKKCGNSQGFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDM 336
           NRHYGFVSYCKKCGNSQ FSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDM
Sbjct: 671 NRHYGFVSYCKKCGNSQAFSWSEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDM 730

Query: 337 LTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSE 396
           LTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSE
Sbjct: 731 LTLAMQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSE 790

Query: 397 MIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQSCLGVHQVT 442
           MIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQSCLGVHQ T
Sbjct: 791 MIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQSCLGVHQAT 835

[Close]

BLAST of CmaCh04G010690 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 395/441 (89.57%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60
           ML+V+  T SPSPFL S QT SH  S+STIHLFQFS S K  LNNCLPNNRTERG+EFDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           G TFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRVAE  G+ RRWMVTNCDANRVM+ FYLQKDFFDFID+DSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300
           EASVLG Y TPLFSYYSYHGPVFRVMLRI+RGR+ KNRHYGF+SYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVS+SLVVSGPLWTGPLHSADYIE+ML+LA QW+WIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLGVHQVT 442
           AECIRIALELQQ+CLG HQVT
Sbjct: 421 AECIRIALELQQNCLGFHQVT 441

[Close]

BLAST of CmaCh04G010690 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 543.9 bits (1400), Expect = 1.2e-154
Identity = 266/432 (61.57%), Postives = 333/432 (77.08%), Query Frame = 0


Query: 1   MLTVMLNTLSPSPFLRSQQTIS-HCFSSSTIHLFQFSQSQKKPLNNCLPNN--RTERGIE 60
           +LT+   TLS S F    ++ +  C S  +       +S K   +  L  +  + ER +E
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRF----KSLKCSFDRILVKSEVQIERNLE 61

Query: 61  FDIGDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLA 120
           F+ G+TFFRHESA GRDLGVL+A LY++S GSLRVLDA+CGCGIRSLRYLVEAEADFV+A
Sbjct: 62  FETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMA 121

Query: 121 NDANDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSD 180
           NDAND+NR VI  NLS+V  G G+ RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSD
Sbjct: 122 NDANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSD 181

Query: 181 STFLRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGG 240
           S+FLR A NAL+L GLLY+TSTDG+SSGGH P  SLAAYGA++RPMPF NEIGLRMLIGG
Sbjct: 182 SSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGG 241

Query: 241 VAREASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSW 300
             REA++LG++VTPLFSYYSYHGPVFRVMLR+ RG+++++R+YGFV++C  CG+S    +
Sbjct: 242 AVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRF 301

Query: 301 SEVGQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKL 360
            E+G M CPC+DT+ S SLVVSGP+W GPLH A Y+ +ML LA +W W+  G G DL+KL
Sbjct: 302 DELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKL 361

Query: 361 LGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTN 420
           L  M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+
Sbjct: 362 LSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTD 421

Query: 421 CPMAECIRIALE 430
           CPM+  +RIA E
Sbjct: 422 CPMSHFVRIAKE 429

[Close]

BLAST of CmaCh04G010690 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 83.2 bits (204), Expect = 5.8e-16
Identity = 83/356 (23.31%), Postives = 147/356 (41.29%), Query Frame = 0


Query: 91  RVLDALCGCGIRSLRYLVEAEA-DFVLANDANDENRGVIVKN--------LSRVAEGFGE 150
           +VL+AL   G+R+LRY  E E    V+A D +  +     +N        +S+V     +
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 151 TRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTFLRPAINALKLDGLLYVTSTDG 210
            R  M+T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTH-------------PKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 211 FSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYVTPLFSYYSYHGP 270
               G +     + YG+Y     + +E+ LR+L+  +   A+    Y+ P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 271 VFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEVGQMSCPCNDT----------- 330
             RV +R+             +SY  +C     F    VG+ S P N++           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 331 -----RVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQMVDES 390
                   +   + GP+W+ P+H  +++  +L      +     +    +++   +   S
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK----DRYPAYDRISAVLTTVS 422

Query: 391 DSKLPVG-YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
           +  L V  ++ +  + +  K  SP      S +I A Y  S +H+    +KT+ PM
Sbjct: 423 EELLDVPLFLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

The following BLAST results are available for this feature:

BLAST of CmaCh04G010690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6YUU9	2.1e-34	31.75	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N...	[more]
Q9V1P3	2.8e-31	27.91	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O...	[more]
P81554	3.6e-31	29.00	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ...	[more]
Q9YDY7	1.4e-30	28.42	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700...	[more]
Q5JIB3	9.0e-30	28.88	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain...	[more]

BLAST of CmaCh04G010690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBD3	3.2e-256	100.00	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1FWD4	2.3e-254	99.09	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A0A0KP99	6.3e-228	89.57	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A1S3C509	5.3e-227	88.66	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A5D3DFE8	5.3e-227	88.66	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]

BLAST of CmaCh04G010690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986521.1	6.6e-256	100.00	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima]	[more]
XP_022943113.1	4.7e-254	99.09	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata]	[more]
XP_023529370.1	1.5e-252	98.87	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo]	[more]
KAG6600883.1	2.7e-233	99.01	Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038892632.1	2.4e-229	89.57	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida]	[more]

BLAST of CmaCh04G010690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56330.1	1.2e-154	61.57	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT3G02320.1	5.8e-16	23.31	N2,N2-dimethylguanosine tRNA methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 64..426 e-value: 2.3E-65 score: 221.2
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 10..432
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 49..427 score: 94.732529
IPR042296	tRNA methyltransferase, Trm1, C-terminal	GENE3D	3.30.56.70		coord: 372..427 e-value: 3.6E-16 score: 60.6
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 33..367 e-value: 6.6E-74 score: 250.5
None	No IPR available	PANTHER	PTHR10631:SF9	TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE	coord: 10..432
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 51..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G010690.1	CmaCh04G010690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002940	tRNA N2-guanine methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0003723	RNA binding