CmaCh04G009940 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh04G009940 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 4.8e-257
Identity = 444/738 (60.16%), Postives = 563/738 (76.29%), Query Frame = 0

Query: 9   VLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVKESYVEAEE 68
           VLV V+L++     + E +  K  +I++L  +P  N  + ++TH+N+L S+  S  EA+E
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGLSSHAKRN 128
             VYSYTK+FNAFAAKL+  EA  + + E+V  V  N+YRKL TT+SWDF+GL   AKR+
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 SK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLIGARYFKL 188
            K E D+I+G+ DTGITP ++SF D G GPPP KWKG+C  + NFTGCN K+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARVAMYKVCW 248
           DGN    ++ SPID+DGHGTHTSST  G  VA ASL G+A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 ASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKGIITVTSA 308
           A +GCADMDILA F+AAIHDGV++ISISI GG   +YS DSIS+G+FHAM+KGI+TV SA
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKKKMYPLVY 368
           GNDGP+ G+V NH PWI+TV AS IDR F S ++LGNGK+ SG+GI++F+PK K YPLV 
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGAIIQSNEY 428
           G D A+   ++  A +C  DSLD  KVKG ++ C +   GV+S + + G  GAII S++Y
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFSSRGPNPG 488
           LDNA IFMAPAT V+SSVG +I+ YI STR+ +AVI KTRQ+   AP  ASFSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 TTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 548
           + R+LKPDIAAPG+DILA +T  +SLTG +GDTQFSKFT++SGTSMACPHVA  AAYVKS
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 FHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLNEMSYIQF 608
           FHP W+P AI+SA+IT+A  ISRR+N D EFAYG G +NP RA +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTVFRRRVTN 668
           LC EGY   +++ L GT+S++CS+++PG GHDSLNYPT QL L+S + +   VFRRRVTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMVSGSVAWI 728
           V  P SVY AT++AP GVEITV P +LSFS+  QKRSFKV VKA  +  GK+VSG + W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARHAVRSPIVVYSPEDE 746
             RH+VRSPIV+YSP  +
Sbjct: 734 SPRHSVRSPIVIYSPTSD 749

BLAST of CmaCh04G009940 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.4e-176
Identity = 364/765 (47.58%), Postives = 478/765 (62.48%), Query Frame = 0

Query: 8   RVLVCVLLFVVGCVDAVEIDE--EKKHFIVFLEMKPGLNEFDAVETHLNVLMSVKESYVE 67
           R+ +     V   V A   DE  E+K +IV++      +  +A E H N+LM+V     +
Sbjct: 8   RLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESK 67

Query: 68  AEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGL--SS 127
           A E  +YSY K+ N F A+L   EA  LS+ E V  V  N  R+L TTRSWDF+GL  S 
Sbjct: 68  ARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESK 127

Query: 128 HAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLIGAR 187
           + +    E++IIVG+ DTGI   + SF D G GPPP KWKG C    NFT CN K+IGA+
Sbjct: 128 YKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAK 187

Query: 188 YFKL------DGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPS 247
           YF +      DG  D A      D DGHGTHTSST  G +V+ ASL G+A GTARGGVPS
Sbjct: 188 YFHIQSEGLPDGEGDTA-----ADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPS 247

Query: 248 ARVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAM 307
           AR+A YKVCW S GC DMD+LAAFD AI DGVD+ISISIGG +   + +D I+IGAFHAM
Sbjct: 248 ARIAAYKVCWDS-GCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHAM 307

Query: 308 KKGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFN 367
           K+GI+T  SAGN+GP   +V N APW++TV A+++DRKF ++++LGNG   SG+ +N FN
Sbjct: 308 KRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFN 367

Query: 368 PKKKMYPLVYGGDVAR-GAGNRESASHCEEDSLDPSKVKGSLVFCELIT-------WGVD 427
           P+KKMYPL  G   +   AG     S CE  +L   KV G +V+CE           G D
Sbjct: 368 PRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQD 427

Query: 428 SVVSALGANGAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL 487
            VV +L   G I+Q  E  D A   +   + V    G  I  YI ST+ P AVI+KT+  
Sbjct: 428 HVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTT 487

Query: 488 KAAAPMAASFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMS 547
           K  AP  +SFS+RGP   +  ILKPDI+APG++ILA Y+ L S+TG   D + + F++MS
Sbjct: 488 KMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMS 547

Query: 548 GTSMACPHVAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSR 607
           GTSMACPH AAAAAYVKSFHP WSP AI+SAL+TTA+ +  + N + E +YG+G +NP R
Sbjct: 548 GTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRR 607

Query: 608 AINPGLIYDLNEMSYIQFLCSEGYTGFSISVLTG-------TKSINCSTLIPGHGHDSLN 667
           AI+PGL+YD+ E +Y++FLC EGY   SI +LTG        K  NC  +  G G D LN
Sbjct: 608 AIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLN 667

Query: 668 YPTFQLRLKSPRRAMSTVFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQK 727
           YP+   ++ S    +S VF R VTNV +  S Y A + AP G+ + V P  +SF R ++K
Sbjct: 668 YPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEK 727

Query: 728 RSFKVAVKASPLASGK-MVSGSVAWIGAR-HAVRSPIVVYSPEDE 746
           R+FKV +      + K +VS SV W  +R H VRSPI+++  +++
Sbjct: 728 RNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFRSDND 764

BLAST of CmaCh04G009940 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 3.7e-161
Identity = 312/717 (43.51%), Postives = 457/717 (63.74%), Query Frame = 0

Query: 27  DEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVKESYVEAEESMVYSYTKSFNAFAAKLT 86
           D+ K  +IV++  K  L + D+   H   ++        A ES++++Y +SFN FA KLT
Sbjct: 28  DDGKNIYIVYMGRK--LEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLT 87

Query: 87  EEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGLSSHAKRNSK-ETDIIVGLFDTGITP 146
           EEEA  ++  E V  V  N   +L TTRSWDF+G      R S+ E++I+VG+ DTGI P
Sbjct: 88  EEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWP 147

Query: 147 TADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLIGARYFKLDGNTDPADILSPIDVDGH 206
            + SF D+GF PPP KWKGTC    NF  CN+K+IGAR + +     P D+  P D +GH
Sbjct: 148 ESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRKIIGARSYHIGRPISPGDVNGPRDTNGH 207

Query: 207 GTHTSSTATGNAVAGASLSGLAKGTARGGVPSARVAMYKVCWASTGCADMDILAAFDAAI 266
           GTHT+STA G  V+ A+L GL  GTARGGVP AR+A YKVCW + GC+D DILAA+D AI
Sbjct: 208 GTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCW-NDGCSDTDILAAYDDAI 267

Query: 267 HDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKGIITVTSAGNDGPTPGSVVNHAPWIV 326
            DGVD+IS+S+GG    +Y  D+I+IG+FHA+++GI+T  SAGN GP   +  + +PW++
Sbjct: 268 ADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLL 327

Query: 327 TVGASAIDRKFISLLELGNGKNISGVGINIFNPKKKMYPLVYGGDVARGAGNRESASHCE 386
           +V AS +DRKF++ +++GNG++  GV IN F+   + YPLV G D+     ++ ++  C 
Sbjct: 328 SVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCT 387

Query: 387 EDSLDPSKVKGSLVFCELITWGVDSVVSALGANGAIIQSNEYLDNANIFMAPATMVSSSV 446
           + S++P+ +KG +V CE  ++G      +L     ++ ++   D A+ +  P++++  + 
Sbjct: 388 DKSVNPNLLKGKIVVCE-ASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPND 447

Query: 447 GAVIHSYIKSTRTPTAVIYK-TRQLKAAAPMAASFSSRGPNPGTTRILKPDIAAPGVDIL 506
                 YI S R+P A I+K T  L A+AP+  SFSSRGPN  T  ++KPDI+ PGV+IL
Sbjct: 448 LLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEIL 507

Query: 507 AGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPGAIRSALITT 566
           A +  +  + G   +T F+   ++SGTSM+CPH+   A YVK+++P WSP AI+SAL+TT
Sbjct: 508 AAWPSVAPVGGIRRNTLFN---IISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTT 567

Query: 567 ASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLNEMSYIQFLCSEGYTGFSISVLTGT 626
           AS ++ R NP  EFAYG+G++NP +A+ PGL+YD NE  Y++FLC +GY   ++  +TG 
Sbjct: 568 ASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGD 627

Query: 627 KSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTVFRRRVTNVSHPVSVYNATIKAPPG 686
            S  C++   G   D LNYP+F L + SP +  +  F R +T+V+   S Y A I AP G
Sbjct: 628 YSA-CTSGNTGRVWD-LNYPSFGLSV-SPSQTFNQYFNRTLTSVAPQASTYRAMISAPQG 687

Query: 687 VEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMVSGSVAWIGARHAVRSPIVVYS 742
           + I+V P+ LSF+ L  ++SF + V+ S    G +VS S+ W    H VRSPI + S
Sbjct: 688 LTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHYVRSPITITS 729

BLAST of CmaCh04G009940 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.3e-158
Identity = 319/737 (43.28%), Postives = 458/737 (62.14%), Query Frame = 0

Query: 10  LVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVKESYVEAEES 69
           L+C+       V A +  +    +IV++   P + ++     HL++L  +  + + A   
Sbjct: 10  LICLAFIFTRDVSANDYRQASSVYIVYMGTLPEI-KYSPPSHHLSILQKLVGT-IAASHL 69

Query: 70  MVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGLSSHAKRNS 129
           +V SY +SFN FAA L++ E+  L   ++V  V P++  +L TTRSWDF+G    A+R S
Sbjct: 70  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 129

Query: 130 -KETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLIGARYFKLD 189
            KE+D+IVG+ D+GI P ++SF D+GFGPPP KWKG+C     F  CN KLIGAR++   
Sbjct: 130 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFYNKF 189

Query: 190 GNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARVAMYKVCWA 249
            +       S  D +GHGTHT+STA GNAV  AS  GLA+GTARGGVPSAR+A YKVC+ 
Sbjct: 190 AD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF- 249

Query: 250 STGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKGIITVTSAG 309
              C D+DILAAFD AI DGVDVISISI      N  + S++IG+FHAM +GIIT  SAG
Sbjct: 250 -NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAG 309

Query: 310 NDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKKKMYPLVYG 369
           N+GP  GSV N +PW++TV AS  DR+FI  + LGNGK ++G+ +N FN     +P+VYG
Sbjct: 310 NNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG 369

Query: 370 GDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGAIIQSNEYL 429
            +V+R   ++  A +C    +D   VKG +V C+      ++ ++  GA G I+Q+    
Sbjct: 370 QNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLA--GAIGVIVQNTLLP 429

Query: 430 DNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL-KAAAPMAASFSSRGPNPG 489
           D+A +   PA+ +       I SYI+S   P A I +T ++    AP   SFSSRGP+  
Sbjct: 430 DSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFV 489

Query: 490 TTRILKPDIAAPGVDILAGYTPLKSLTG--QEGDTQFSKFTLMSGTSMACPHVAAAAAYV 549
              +LKPD++APG++ILA ++P+ S +      D +  ++++MSGTSMACPHVA  AAYV
Sbjct: 490 IQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYV 549

Query: 550 KSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLNEMSYI 609
           KSFHP WSP AI+SA++TTA+ ++ + NP+ EFAYG+G +NP++A +PGL+Y++    Y+
Sbjct: 550 KSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYL 609

Query: 610 QFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTVFRRRV 669
           + LC+EG+   +++  +G +++ CS          LNYPT    + S     +  F+R V
Sbjct: 610 KMLCAEGFDSTTLTTTSG-QNVTCSERTE---VKDLNYPTMTTFVSS-LDPFNVTFKRTV 669

Query: 670 TNVSHPVSVYNAT-IKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMVSGSV 729
           TNV  P S Y A+ +   P ++I++ P  L F  L++K+SF V +    L  G  VS SV
Sbjct: 670 TNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSV 726

Query: 730 AWIGARHAVRSPIVVYS 742
            W    H+VRSPIV YS
Sbjct: 730 VWSDGSHSVRSPIVAYS 726

BLAST of CmaCh04G009940 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 4.7e-156
Identity = 332/753 (44.09%), Postives = 461/753 (61.22%), Query Frame = 0

Query: 3   ISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK-E 62
           ++ SS +L C+L+  +  V AV   ++K+ +IV++       ++     H+N+L  V  E
Sbjct: 4   LAASSSLLSCLLVLFLSSVSAV--TDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGE 63

Query: 63  SYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGL 122
           S +E    +V SY +SFN FAA+LTE E   ++K   V  V PN+  +LQTT SWDF+GL
Sbjct: 64  SSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGL 123

Query: 123 SS--HAKRN-SKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKK 182
                 KRN + E+D I+G+ D+GITP + SF D GFGPPP KWKG C    NFT CN K
Sbjct: 124 KEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNNK 183

Query: 183 LIGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSA 242
           LIGAR +  +G           D+DGHGTHT+STA GNAV  AS  G+  GT RGGVP++
Sbjct: 184 LIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 243

Query: 243 RVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMK 302
           RVA YKVC   TGC+   +L+AFD AI DGVD+I+ISIG      + +D I+IGAFHAM 
Sbjct: 244 RVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMA 303

Query: 303 KGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNP 362
           KG++TV SAGN GP P SV   APWI+TV AS  +R F++ + LGNGK + G  +N +  
Sbjct: 304 KGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEM 363

Query: 363 KKKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGAN 422
           K K YPLVYG   A  A + ESA  CE   +D S+VKG ++ C     G   +V ++GA 
Sbjct: 364 KGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGAV 423

Query: 423 GAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL-KAAAPMAA 482
           G I ++ +  D A I   PA  + +     + SY++ST +P A++ KT  +    +P+ A
Sbjct: 424 GLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 483

Query: 483 SFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPH 542
           SFSSRGPN     ILKPDI APGV+ILA Y+P       + DT+  K++++SGTSM+CPH
Sbjct: 484 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGE--PSQDDTRHVKYSVLSGTSMSCPH 543

Query: 543 VAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLN--PDGEFAYGAGNLNPSRAINPGL 602
           VA  AAYVK+F+P WSP  I+SA++TTA  ++         EFAYG+G+++P  A NPGL
Sbjct: 544 VAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGL 603

Query: 603 IYDLNEMSYIQFLCSEGYTGFSISVLTGTKSINCS---TLIPGHGHDSLNYPTFQLRLKS 662
           +Y+L++  +I FLC   YT   + V++G +++ CS    ++P     +LNYP+   +L  
Sbjct: 604 VYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILP----RNLNYPSMSAKLSG 663

Query: 663 PRRAMSTVFRRRVTNVSHPVSVYNATIKAPPG--VEITVTPSTLSFSRLQQKRSFKVAVK 722
                +  F R +TNV  P S Y + + A  G  +++ +TPS LSF  + +K+SF V V 
Sbjct: 664 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 723

Query: 723 ASPLASGKMVSGSVAWIGARHAVRSPIVVYSPE 744
            S L S    S ++ W    H VRSPIVVY+ +
Sbjct: 724 GSNLDSEVPSSANLIWSDGTHNVRSPIVVYTSD 731

BLAST of CmaCh04G009940 vs. ExPASy TrEMBL
Match: A0A6J1IN55 (subtilisin-like protease SBT4.14 OS=Cucurbita maxima OX=3661 GN=LOC111477941 PE=3 SV=1)

HSP 1 Score: 1475.7 bits (3819), Expect = 0.0e+00
Identity = 751/751 (100.00%), Postives = 751/751 (100.00%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120

Query: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180
           LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI
Sbjct: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180

Query: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240
           GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV
Sbjct: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240

Query: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300
           AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG
Sbjct: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300

Query: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360
           IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK
Sbjct: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360

Query: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420
           KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA
Sbjct: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420

Query: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480
           IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS
Sbjct: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480

Query: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540
           SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA
Sbjct: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540

Query: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600
           AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN
Sbjct: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600

Query: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660
           EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV
Sbjct: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660

Query: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720
           FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV
Sbjct: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720

Query: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE
Sbjct: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 751

BLAST of CmaCh04G009940 vs. ExPASy TrEMBL
Match: A0A6J1FLU9 (subtilisin-like protease SBT4.14 OS=Cucurbita moschata OX=3662 GN=LOC111446986 PE=3 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 724/751 (96.40%), Postives = 736/751 (98.00%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFV+GCV AVEIDEEKKHFIVFLEMKP LNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           ESYVEAEESMVYSYTKSFNAFAAKLTE+EALALSKREDVHHVIPNRYRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180
           L S A+RNSKETDIIVGLFDTGITPTADSFKDDGFGPPP KWKGTCHHFANFTGCN+KLI
Sbjct: 121 LPSKARRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKLI 180

Query: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240
           GARYFKLDGN DPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV
Sbjct: 181 GARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240

Query: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300
           AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHA+KKG
Sbjct: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKKG 300

Query: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360
           IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGIN FNPKK
Sbjct: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPKK 360

Query: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420
           KMYPLVYGGDVARGA NRESASHC EDSLDPSKVKGSLVFCELITWGVDSVV+ALGANGA
Sbjct: 361 KMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANGA 420

Query: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480
           IIQS+EYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS
Sbjct: 421 IIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480

Query: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540
           SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA
Sbjct: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540

Query: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600
           AAAYVKSFHPLWSPGAIRSALITTAS+ISR+LNPDGEFAYGAGNLNPSRAINPGLIYDLN
Sbjct: 541 AAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600

Query: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660
           EMSYIQFLCSEGYTG SI VL GTKSINCSTLIPGHGHDSLNYPTFQLRLKSP+RAMSTV
Sbjct: 601 EMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMSTV 660

Query: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720
           FRRRVTNV HPVSVYNATIKAPPGVEITVTPSTLSFS+LQQKRSFKVAVKA PLASGKMV
Sbjct: 661 FRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKMV 720

Query: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           SGSVAWIGARHAVRSPIVVYSPEDEEVFVR+
Sbjct: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRD 751

BLAST of CmaCh04G009940 vs. ExPASy TrEMBL
Match: A0A0A0KME6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G000010 PE=3 SV=1)

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 617/743 (83.04%), Postives = 677/743 (91.12%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           M IS SSR+LV  L  VVGCV  ++ DEEK H+IVFLE KP LNE D VETHLN+LMSVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           +S+ EA ESMVYSYTKSFNAFAAKL+++EA  LS R+DVHHVIPN+YRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LSSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKL 180
           LSS+A+R++K E+DIIVGLFDTGITPTADSFKDDGFGPPP KWKGTCHHFANFT CNKKL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDGN DP+DILSP+D DGHGTHTSSTATGNA+AGASLSGLA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360
           GIITVTSAGN GPT GSVVNHAPWIVTV AS+IDRKFIS LELGNGKNISGVGINIFNPK
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 KKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANG 420
           +KMYPLV GGDVAR + ++++AS C E +LDP+KVKGSLVFC+L+TWG DSV+ ++GANG
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 AIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480
            IIQS+E+LDNA+IFMAPATMVSS VG +I++YIKSTRTPTAVIYKT+QLKA APM ASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPG+ RILKPDIAAPGVDILA YTPLKSLTGQ+GDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600
           AAAAYVKSFHPLWSP AIRSAL+TTA+ ISRRLNP+GEFAYGAGNLNPSRAI+PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660
           NE+SYIQFLCSEGYTG SI+VL+GTKSINCS LIPG GHDSLNYPTFQL LKS  + M+T
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKM 720
            FRRRVTNV HP+SVYNATI APPGV ITVTP TLSFSRL QKRSFKV VKASPL S KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSVAWIGARHAVRSPIVVYSP 743
           VSGS+AW+GA+H VRSPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of CmaCh04G009940 vs. ExPASy TrEMBL
Match: A0A5A7UKY8 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002590 PE=3 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 620/747 (83.00%), Postives = 679/747 (90.90%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCV----DAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVL 60
           M +S SSR+LV  L  +VGCV    D  E +EEK HFIVFL+ KP LNE DAVETHL+VL
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDE-NEEKNHFIVFLQNKPVLNEVDAVETHLSVL 60

Query: 61  MSVKESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSW 120
           MSVK+S+ EA ESMVYSYTKSFNAFAAKL++ EA  LS R+DVHHVIPN YRKLQTTRSW
Sbjct: 61  MSVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSW 120

Query: 121 DFIGLSSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGC 180
           DFIGLSS+A+R +K E+DIIVGLFDTGITPTADSF+DDGFGPPP KWKGTCHHFANFT C
Sbjct: 121 DFIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTAC 180

Query: 181 NKKLIGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGV 240
           NKKLIGARYFKLDGN DP+DILSP+D DGHGTHTSSTATGNAVAGASLSGLA+GTARGGV
Sbjct: 181 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGV 240

Query: 241 PSARVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFH 300
           PSARVAMYKVCWAS+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFH
Sbjct: 241 PSARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 300

Query: 301 AMKKGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINI 360
           AMKKGIITVTSAGN GP  GSVVNHAPWIVTV AS+IDRKFIS LELGNGKNISGVGINI
Sbjct: 301 AMKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 360

Query: 361 FNPKKKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSAL 420
           FNPKKKMYPLV GGDVAR + ++++AS C EDSLDP+KVKGSLVFC+L+TWG DSV+ ++
Sbjct: 361 FNPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSV 420

Query: 421 GANGAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPM 480
           GANG IIQS+++LDNA+IFMAPATMVSSSVG +I++YIKSTRTPTAVIYKTRQLKA APM
Sbjct: 421 GANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPM 480

Query: 481 AASFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMAC 540
            ASFSSRGPNPG+ RILKPDIAAPGV+ILAGYTPLKSLTGQ+GDTQ+SKFT+MSGTSMAC
Sbjct: 481 VASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMAC 540

Query: 541 PHVAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGL 600
           PHVAAAAAYVKSFHPLWSP AIRSAL+TT   ISRRLNP+GEFAYGAGNLNPS+AI+PGL
Sbjct: 541 PHVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGL 600

Query: 601 IYDLNEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRR 660
           IYDLNEMSYIQFLCSEGYTG SI+VL GTKSINCSTLIPGHGHDSLNYPTFQL L+S ++
Sbjct: 601 IYDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQ 660

Query: 661 AMSTVFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLA 720
            M+T FRR+VTNV  PVSVYNATIKAPPGV+ITVTP+TLSFSRL QKRSFKV VKASPL 
Sbjct: 661 PMTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQ 720

Query: 721 SGKMVSGSVAWIGARHAVRSPIVVYSP 743
           S KMVSGS+AW+G RH VRSPIVVYSP
Sbjct: 721 SAKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of CmaCh04G009940 vs. ExPASy TrEMBL
Match: A0A1S3C036 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495082 PE=3 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 620/747 (83.00%), Postives = 679/747 (90.90%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCV----DAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVL 60
           M +S SSR+LV  L  +VGCV    D  E +EEK HFIVFL+ KP LNE DAVETHL+VL
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDE-NEEKNHFIVFLQNKPVLNEVDAVETHLSVL 60

Query: 61  MSVKESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSW 120
           MSVK+S+ EA ESMVYSYTKSFNAFAAKL++ EA  LS R+DVHHVIPN YRKLQTTRSW
Sbjct: 61  MSVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSW 120

Query: 121 DFIGLSSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGC 180
           DFIGLSS+A+R +K E+DIIVGLFDTGITPTADSF+DDGFGPPP KWKGTCHHFANFT C
Sbjct: 121 DFIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTAC 180

Query: 181 NKKLIGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGV 240
           NKKLIGARYFKLDGN DP+DILSP+D DGHGTHTSSTATGNAVAGASLSGLA+GTARGGV
Sbjct: 181 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGV 240

Query: 241 PSARVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFH 300
           PSARVAMYKVCWAS+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFH
Sbjct: 241 PSARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 300

Query: 301 AMKKGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINI 360
           AMKKGIITVTSAGN GP  GSVVNHAPWIVTV AS+IDRKFIS LELGNGKNISGVGINI
Sbjct: 301 AMKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 360

Query: 361 FNPKKKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSAL 420
           FNPKKKMYPLV GGDVAR + ++++AS C EDSLDP+KVKGSLVFC+L+TWG DSV+ ++
Sbjct: 361 FNPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSV 420

Query: 421 GANGAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPM 480
           GANG IIQS+++LDNA+IFMAPATMVSSSVG +I++YIKSTRTPTAVIYKTRQLKA APM
Sbjct: 421 GANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPM 480

Query: 481 AASFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMAC 540
            ASFSSRGPNPG+ RILKPDIAAPGV+ILAGYTPLKSLTGQ+GDTQ+SKFT+MSGTSMAC
Sbjct: 481 VASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMAC 540

Query: 541 PHVAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGL 600
           PHVAAAAAYVKSFHPLWSP AIRSAL+TT   ISRRLNP+GEFAYGAGNLNPS+AI+PGL
Sbjct: 541 PHVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGL 600

Query: 601 IYDLNEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRR 660
           IYDLNEMSYIQFLCSEGYTG SI+VL GTKSINCSTLIPGHGHDSLNYPTFQL L+S ++
Sbjct: 601 IYDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQ 660

Query: 661 AMSTVFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLA 720
            M+T FRR+VTNV  PVSVYNATIKAPPGV+ITVTP+TLSFSRL QKRSFKV VKASPL 
Sbjct: 661 PMTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQ 720

Query: 721 SGKMVSGSVAWIGARHAVRSPIVVYSP 743
           S KMVSGS+AW+G RH VRSPIVVYSP
Sbjct: 721 SAKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of CmaCh04G009940 vs. NCBI nr
Match: XP_022977720.1 (subtilisin-like protease SBT4.14 [Cucurbita maxima])

HSP 1 Score: 1475.7 bits (3819), Expect = 0.0e+00
Identity = 751/751 (100.00%), Postives = 751/751 (100.00%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120

Query: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180
           LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI
Sbjct: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180

Query: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240
           GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV
Sbjct: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240

Query: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300
           AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG
Sbjct: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300

Query: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360
           IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK
Sbjct: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360

Query: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420
           KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA
Sbjct: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420

Query: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480
           IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS
Sbjct: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480

Query: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540
           SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA
Sbjct: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540

Query: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600
           AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN
Sbjct: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600

Query: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660
           EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV
Sbjct: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660

Query: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720
           FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV
Sbjct: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720

Query: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE
Sbjct: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 751

BLAST of CmaCh04G009940 vs. NCBI nr
Match: KAG6600817.1 (Subtilisin-like protease 4.14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 729/751 (97.07%), Postives = 736/751 (98.00%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFVVGCV AVEIDEEKKHFIVFLEMKP LNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           ESYVEAEESMVYSYTKSFNAFAAKLTE+EALALSKREDVHHVIPNRYRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120

Query: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180
           L S A RNSKETDIIVGLFDTGITPTADSFKDDGFGPPP KWKGTCHHFANFTGCN+KLI
Sbjct: 121 LPSKAGRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNRKLI 180

Query: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240
           GARYFKLDGN DPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV
Sbjct: 181 GARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240

Query: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300
           AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHA+KKG
Sbjct: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKKG 300

Query: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360
           IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK
Sbjct: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360

Query: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420
           KMYPLVYGGDVARGA NRESASHC EDSLDPSKVKGSLVFCELITWGVDSVV+ALGANGA
Sbjct: 361 KMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANGA 420

Query: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480
           IIQS+EYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS
Sbjct: 421 IIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480

Query: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540
           SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA
Sbjct: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540

Query: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600
           AAAYVKSFHPLWSPGAIRSALITTAS+ISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN
Sbjct: 541 AAAYVKSFHPLWSPGAIRSALITTASRISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600

Query: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660
           EMSYIQFLCSEGYTG SI VL GTK INCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV
Sbjct: 601 EMSYIQFLCSEGYTGSSIGVLAGTKPINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660

Query: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720
           FRRRVTNV HPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKA PLASGKMV
Sbjct: 661 FRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKAGPLASGKMV 720

Query: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           SGSVAWIGARHAVRSPIVVYSPEDEEVFVR+
Sbjct: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRD 751

BLAST of CmaCh04G009940 vs. NCBI nr
Match: XP_023550181.1 (subtilisin-like protease SBT4.14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 727/751 (96.80%), Postives = 734/751 (97.74%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFVVGCV AVEIDEEKKHFIVFLEMKP LNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           ESYVEAEESMVYSYTKSFNAFAAKL EEEALALSKREDVHHVIPN+YRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLNEEEALALSKREDVHHVIPNQYRKLQTTRSWDFIG 120

Query: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180
           L S A+RNSKETDIIVGLFDTGITPTADSFKDDGFGPPP KWKGTCHHFANFTGCN+KLI
Sbjct: 121 LPSKARRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNRKLI 180

Query: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240
           GARYFKLDGN DPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV
Sbjct: 181 GARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240

Query: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300
           AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHA+KKG
Sbjct: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKKG 300

Query: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360
           IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK
Sbjct: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360

Query: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420
           KMYPLVYGGDVARGA NRESASHC EDSLDPSKVKGSLVFCELITWGVDSVV+ALGANGA
Sbjct: 361 KMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANGA 420

Query: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480
           IIQS+EYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS
Sbjct: 421 IIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480

Query: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540
           SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA
Sbjct: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540

Query: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600
           AAAYVKSFHPLWSP AIRSALITTAS+ISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN
Sbjct: 541 AAAYVKSFHPLWSPAAIRSALITTASRISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600

Query: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660
           EMSYIQFLC EGYTG SI VL GTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV
Sbjct: 601 EMSYIQFLCREGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660

Query: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720
           FRRRVTNV HPVSVYNATIKAPPGVEITVTPSTLSF RLQQKRSFKVAVKA PLASGKMV
Sbjct: 661 FRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFLRLQQKRSFKVAVKAGPLASGKMV 720

Query: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE
Sbjct: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 751

BLAST of CmaCh04G009940 vs. NCBI nr
Match: KAG7031454.1 (Subtilisin-like protease SBT4.14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 729/752 (96.94%), Postives = 736/752 (97.87%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFVVGCV AVEIDEEKKHFIVFLEMKP LNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  E-SYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFI 120
           E SYVEAEESMVYSYTKSFNAFAAKLTE+EALALSKREDVHHVIPNRYRKLQTTRSWDFI
Sbjct: 61  ESSYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFI 120

Query: 121 GLSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKL 180
           GL S A RNSKETDIIVGLFDTGITPTADSFKDDGFGPPP KWKGTCHHFANFTGCN+KL
Sbjct: 121 GLPSKAGRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNRKL 180

Query: 181 IGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDGN DPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKK 300
           VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360
           GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360

Query: 361 KKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANG 420
           KKMYPLVYGGDVARGA NRESASHC EDSLDPSKVKGSLVFCELITWGVDSVV+ALGANG
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 AIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480
           AIIQS+EYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600
           AAAAYVKSFHPLWSPGAIRSALITTAS+ISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASRISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660
           NEMSYIQFLCSEGYTG SI VL GTK INCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST
Sbjct: 601 NEMSYIQFLCSEGYTGSSIGVLAGTKPINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660

Query: 661 VFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKM 720
           VFRRRVTNV HPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKA PLASGKM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           VSGSVAWIGARHAVRSPIVVYSPEDEEVFVR+
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSPEDEEVFVRD 752

BLAST of CmaCh04G009940 vs. NCBI nr
Match: XP_022941706.1 (subtilisin-like protease SBT4.14 [Cucurbita moschata])

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 724/751 (96.40%), Postives = 736/751 (98.00%), Query Frame = 0

Query: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60
           MLISISSRVLVCVLLFV+GCV AVEIDEEKKHFIVFLEMKP LNEFDAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120
           ESYVEAEESMVYSYTKSFNAFAAKLTE+EALALSKREDVHHVIPNRYRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LSSHAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLI 180
           L S A+RNSKETDIIVGLFDTGITPTADSFKDDGFGPPP KWKGTCHHFANFTGCN+KLI
Sbjct: 121 LPSKARRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKLI 180

Query: 181 GARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240
           GARYFKLDGN DPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV
Sbjct: 181 GARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARV 240

Query: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKG 300
           AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHA+KKG
Sbjct: 241 AMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKKG 300

Query: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKK 360
           IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGIN FNPKK
Sbjct: 301 IITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPKK 360

Query: 361 KMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGA 420
           KMYPLVYGGDVARGA NRESASHC EDSLDPSKVKGSLVFCELITWGVDSVV+ALGANGA
Sbjct: 361 KMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANGA 420

Query: 421 IIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480
           IIQS+EYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS
Sbjct: 421 IIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFS 480

Query: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540
           SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA
Sbjct: 481 SRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAA 540

Query: 541 AAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600
           AAAYVKSFHPLWSPGAIRSALITTAS+ISR+LNPDGEFAYGAGNLNPSRAINPGLIYDLN
Sbjct: 541 AAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDLN 600

Query: 601 EMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTV 660
           EMSYIQFLCSEGYTG SI VL GTKSINCSTLIPGHGHDSLNYPTFQLRLKSP+RAMSTV
Sbjct: 601 EMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMSTV 660

Query: 661 FRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMV 720
           FRRRVTNV HPVSVYNATIKAPPGVEITVTPSTLSFS+LQQKRSFKVAVKA PLASGKMV
Sbjct: 661 FRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKMV 720

Query: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRE 752
           SGSVAWIGARHAVRSPIVVYSPEDEEVFVR+
Sbjct: 721 SGSVAWIGARHAVRSPIVVYSPEDEEVFVRD 751

BLAST of CmaCh04G009940 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 888.6 bits (2295), Expect = 3.4e-258
Identity = 444/738 (60.16%), Postives = 563/738 (76.29%), Query Frame = 0

Query: 9   VLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVKESYVEAEE 68
           VLV V+L++     + E +  K  +I++L  +P  N  + ++TH+N+L S+  S  EA+E
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGLSSHAKRN 128
             VYSYTK+FNAFAAKL+  EA  + + E+V  V  N+YRKL TT+SWDF+GL   AKR+
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 SK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLIGARYFKL 188
            K E D+I+G+ DTGITP ++SF D G GPPP KWKG+C  + NFTGCN K+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSARVAMYKVCW 248
           DGN    ++ SPID+DGHGTHTSST  G  VA ASL G+A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 ASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKKGIITVTSA 308
           A +GCADMDILA F+AAIHDGV++ISISI GG   +YS DSIS+G+FHAM+KGI+TV SA
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPKKKMYPLVY 368
           GNDGP+ G+V NH PWI+TV AS IDR F S ++LGNGK+ SG+GI++F+PK K YPLV 
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANGAIIQSNEY 428
           G D A+   ++  A +C  DSLD  KVKG ++ C +   GV+S + + G  GAII S++Y
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASFSSRGPNPG 488
           LDNA IFMAPAT V+SSVG +I+ YI STR+ +AVI KTRQ+   AP  ASFSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 TTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 548
           + R+LKPDIAAPG+DILA +T  +SLTG +GDTQFSKFT++SGTSMACPHVA  AAYVKS
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 FHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDLNEMSYIQF 608
           FHP W+P AI+SA+IT+A  ISRR+N D EFAYG G +NP RA +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMSTVFRRRVTN 668
           LC EGY   +++ L GT+S++CS+++PG GHDSLNYPT QL L+S + +   VFRRRVTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKMVSGSVAWI 728
           V  P SVY AT++AP GVEITV P +LSFS+  QKRSFKV VKA  +  GK+VSG + W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARHAVRSPIVVYSPEDE 746
             RH+VRSPIV+YSP  +
Sbjct: 734 SPRHSVRSPIVIYSPTSD 749

BLAST of CmaCh04G009940 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 621.3 bits (1601), Expect = 1.0e-177
Identity = 364/765 (47.58%), Postives = 478/765 (62.48%), Query Frame = 0

Query: 8   RVLVCVLLFVVGCVDAVEIDE--EKKHFIVFLEMKPGLNEFDAVETHLNVLMSVKESYVE 67
           R+ +     V   V A   DE  E+K +IV++      +  +A E H N+LM+V     +
Sbjct: 8   RLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESK 67

Query: 68  AEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGL--SS 127
           A E  +YSY K+ N F A+L   EA  LS+ E V  V  N  R+L TTRSWDF+GL  S 
Sbjct: 68  ARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESK 127

Query: 128 HAKRNSKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKLIGAR 187
           + +    E++IIVG+ DTGI   + SF D G GPPP KWKG C    NFT CN K+IGA+
Sbjct: 128 YKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAK 187

Query: 188 YFKL------DGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPS 247
           YF +      DG  D A      D DGHGTHTSST  G +V+ ASL G+A GTARGGVPS
Sbjct: 188 YFHIQSEGLPDGEGDTA-----ADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPS 247

Query: 248 ARVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAM 307
           AR+A YKVCW S GC DMD+LAAFD AI DGVD+ISISIGG +   + +D I+IGAFHAM
Sbjct: 248 ARIAAYKVCWDS-GCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHAM 307

Query: 308 KKGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFN 367
           K+GI+T  SAGN+GP   +V N APW++TV A+++DRKF ++++LGNG   SG+ +N FN
Sbjct: 308 KRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFN 367

Query: 368 PKKKMYPLVYGGDVAR-GAGNRESASHCEEDSLDPSKVKGSLVFCELIT-------WGVD 427
           P+KKMYPL  G   +   AG     S CE  +L   KV G +V+CE           G D
Sbjct: 368 PRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQD 427

Query: 428 SVVSALGANGAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL 487
            VV +L   G I+Q  E  D A   +   + V    G  I  YI ST+ P AVI+KT+  
Sbjct: 428 HVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTT 487

Query: 488 KAAAPMAASFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMS 547
           K  AP  +SFS+RGP   +  ILKPDI+APG++ILA Y+ L S+TG   D + + F++MS
Sbjct: 488 KMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMS 547

Query: 548 GTSMACPHVAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSR 607
           GTSMACPH AAAAAYVKSFHP WSP AI+SAL+TTA+ +  + N + E +YG+G +NP R
Sbjct: 548 GTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRR 607

Query: 608 AINPGLIYDLNEMSYIQFLCSEGYTGFSISVLTG-------TKSINCSTLIPGHGHDSLN 667
           AI+PGL+YD+ E +Y++FLC EGY   SI +LTG        K  NC  +  G G D LN
Sbjct: 608 AIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLN 667

Query: 668 YPTFQLRLKSPRRAMSTVFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQK 727
           YP+   ++ S    +S VF R VTNV +  S Y A + AP G+ + V P  +SF R ++K
Sbjct: 668 YPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEK 727

Query: 728 RSFKVAVKASPLASGK-MVSGSVAWIGAR-HAVRSPIVVYSPEDE 746
           R+FKV +      + K +VS SV W  +R H VRSPI+++  +++
Sbjct: 728 RNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLFRSDND 764

BLAST of CmaCh04G009940 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 558.1 bits (1437), Expect = 1.0e-158
Identity = 312/695 (44.89%), Postives = 441/695 (63.45%), Query Frame = 0

Query: 52  HLNVLMSVKESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQ 111
           HL++L  +  + + A   +V SY +SFN FAA L++ E+  L   ++V  V P++  +L 
Sbjct: 15  HLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 74

Query: 112 TTRSWDFIGLSSHAKRNS-KETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFA 171
           TTRSWDF+G    A+R S KE+D+IVG+ D+GI P ++SF D+GFGPPP KWKG+C    
Sbjct: 75  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 134

Query: 172 NFTGCNKKLIGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGT 231
            F  CN KLIGAR++    +       S  D +GHGTHT+STA GNAV  AS  GLA+GT
Sbjct: 135 KF-ACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGT 194

Query: 232 ARGGVPSARVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSIS 291
           ARGGVPSAR+A YKVC+    C D+DILAAFD AI DGVDVISISI      N  + S++
Sbjct: 195 ARGGVPSARIAAYKVCF--NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVA 254

Query: 292 IGAFHAMKKGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISG 351
           IG+FHAM +GIIT  SAGN+GP  GSV N +PW++TV AS  DR+FI  + LGNGK ++G
Sbjct: 255 IGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTG 314

Query: 352 VGINIFNPKKKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDS 411
           + +N FN     +P+VYG +V+R   ++  A +C    +D   VKG +V C+      ++
Sbjct: 315 ISVNTFNLNGTKFPIVYGQNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREA 374

Query: 412 VVSALGANGAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL- 471
            ++  GA G I+Q+    D+A +   PA+ +       I SYI+S   P A I +T ++ 
Sbjct: 375 YLA--GAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIV 434

Query: 472 KAAAPMAASFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTG--QEGDTQFSKFTL 531
              AP   SFSSRGP+     +LKPD++APG++ILA ++P+ S +      D +  ++++
Sbjct: 435 DREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSV 494

Query: 532 MSGTSMACPHVAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNP 591
           MSGTSMACPHVA  AAYVKSFHP WSP AI+SA++TTA+ ++ + NP+ EFAYG+G +NP
Sbjct: 495 MSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINP 554

Query: 592 SRAINPGLIYDLNEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQ 651
           ++A +PGL+Y++    Y++ LC+EG+   +++  +G +++ CS          LNYPT  
Sbjct: 555 TKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTE---VKDLNYPTMT 614

Query: 652 LRLKSPRRAMSTVFRRRVTNVSHPVSVYNAT-IKAPPGVEITVTPSTLSFSRLQQKRSFK 711
             + S     +  F+R VTNV  P S Y A+ +   P ++I++ P  L F  L++K+SF 
Sbjct: 615 TFVSS-LDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFV 674

Query: 712 VAVKASPLASGKMVSGSVAWIGARHAVRSPIVVYS 742
           V +    L  G  VS SV W    H+VRSPIV YS
Sbjct: 675 VTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of CmaCh04G009940 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-157
Identity = 332/753 (44.09%), Postives = 461/753 (61.22%), Query Frame = 0

Query: 3   ISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK-E 62
           ++ SS +L C+L+  +  V AV   ++K+ +IV++       ++     H+N+L  V  E
Sbjct: 4   LAASSSLLSCLLVLFLSSVSAV--TDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGE 63

Query: 63  SYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIGL 122
           S +E    +V SY +SFN FAA+LTE E   ++K   V  V PN+  +LQTT SWDF+GL
Sbjct: 64  SSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGL 123

Query: 123 SS--HAKRN-SKETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKK 182
                 KRN + E+D I+G+ D+GITP + SF D GFGPPP KWKG C    NFT CN K
Sbjct: 124 KEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNNK 183

Query: 183 LIGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSA 242
           LIGAR +  +G           D+DGHGTHT+STA GNAV  AS  G+  GT RGGVP++
Sbjct: 184 LIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 243

Query: 243 RVAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMK 302
           RVA YKVC   TGC+   +L+AFD AI DGVD+I+ISIG      + +D I+IGAFHAM 
Sbjct: 244 RVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMA 303

Query: 303 KGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNP 362
           KG++TV SAGN GP P SV   APWI+TV AS  +R F++ + LGNGK + G  +N +  
Sbjct: 304 KGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEM 363

Query: 363 KKKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGAN 422
           K K YPLVYG   A  A + ESA  CE   +D S+VKG ++ C     G   +V ++GA 
Sbjct: 364 KGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGAV 423

Query: 423 GAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL-KAAAPMAA 482
           G I ++ +  D A I   PA  + +     + SY++ST +P A++ KT  +    +P+ A
Sbjct: 424 GLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 483

Query: 483 SFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPH 542
           SFSSRGPN     ILKPDI APGV+ILA Y+P       + DT+  K++++SGTSM+CPH
Sbjct: 484 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGE--PSQDDTRHVKYSVLSGTSMSCPH 543

Query: 543 VAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLN--PDGEFAYGAGNLNPSRAINPGL 602
           VA  AAYVK+F+P WSP  I+SA++TTA  ++         EFAYG+G+++P  A NPGL
Sbjct: 544 VAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGL 603

Query: 603 IYDLNEMSYIQFLCSEGYTGFSISVLTGTKSINCS---TLIPGHGHDSLNYPTFQLRLKS 662
           +Y+L++  +I FLC   YT   + V++G +++ CS    ++P     +LNYP+   +L  
Sbjct: 604 VYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILP----RNLNYPSMSAKLSG 663

Query: 663 PRRAMSTVFRRRVTNVSHPVSVYNATIKAPPG--VEITVTPSTLSFSRLQQKRSFKVAVK 722
                +  F R +TNV  P S Y + + A  G  +++ +TPS LSF  + +K+SF V V 
Sbjct: 664 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 723

Query: 723 ASPLASGKMVSGSVAWIGARHAVRSPIVVYSPE 744
            S L S    S ++ W    H VRSPIVVY+ +
Sbjct: 724 GSNLDSEVPSSANLIWSDGTHNVRSPIVVYTSD 731

BLAST of CmaCh04G009940 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 545.4 bits (1404), Expect = 7.0e-155
Identity = 325/753 (43.16%), Postives = 459/753 (60.96%), Query Frame = 0

Query: 1   MLISISSRVLVCVL-LFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSV 60
           M  ++S  +L C+  L VV    A + D++K+ +IV++   P   ++  +  H ++L  V
Sbjct: 1   MATAVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDV 60

Query: 61  K-ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDF 120
             ES +  ++ +V +Y +SFN FAA+LTE E   L+  ++V  V P++   LQTT SW+F
Sbjct: 61  TGESSI--QDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNF 120

Query: 121 IGL--SSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGC 180
           +GL      KRN   E+D I+G+ D+GI P +DSF   GFGPPP KWKG C    NFT C
Sbjct: 121 MGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-C 180

Query: 181 NKKLIGARYF--KLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARG 240
           N KLIGARY+  KL+G  + A      D  GHG+HT+S A GNAV   S  GL  GT RG
Sbjct: 181 NNKLIGARYYTPKLEGFPESAR-----DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRG 240

Query: 241 GVPSARVAMYKVCWAST-GCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIG 300
           GVP+AR+A+YKVC      C    ILAAFD AI D VD+I++S+G  A G + +D+++IG
Sbjct: 241 GVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIG 300

Query: 301 AFHAMKKGIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVG 360
           AFHAM KGI+TV  AGN+GP   ++V+ APW+ TV AS ++R FI+ + LGNGK I G  
Sbjct: 301 AFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRS 360

Query: 361 INIFNPKKKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVV 420
           +N F+   K YPLVYG   A    +  SA  C    LD  +VKG +V C+  T       
Sbjct: 361 VNSFDLNGKKYPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEA 420

Query: 421 SALGANGAIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQL-KA 480
            A+GA  +I++ N Y D A++F  P +++S     ++ SY+ ST+ P A + K+  +   
Sbjct: 421 QAMGAVASIVR-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQ 480

Query: 481 AAPMAASFSSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGT 540
            AP+ AS+SSRGPNP    ILKPDI APG +ILA Y+P   +   E DT+  K+T++SGT
Sbjct: 481 KAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGT 540

Query: 541 SMACPHVAAAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPD---GEFAYGAGNLNPS 600
           SM+CPHVA  AAY+K+FHPLWSP  I+SA++TTA  ++   +P     EFAYGAG+++P 
Sbjct: 541 SMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPI 600

Query: 601 RAINPGLIYDLNEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQL 660
            AI+PGL+Y+ N+  +I FLC   YTG  + +++G  S +C+         +LNYP+   
Sbjct: 601 AAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSA 660

Query: 661 RLKSPRRAMSTVFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVA 720
           ++ S  +     FRR VTNV  P + Y A +     +++ V P+ LS   L +K+SF V 
Sbjct: 661 QV-SGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVT 720

Query: 721 VKASPLASGKMVSGSVAWIGARHAVRSPIVVYS 742
           V  +   +  +VS  + W    H VRSPIVVY+
Sbjct: 721 VSGAGPKAENLVSAQLIWSDGVHFVRSPIVVYA 736