Homology
BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 932.6 bits (2409), Expect = 2.8e-270
Identity = 467/737 (63.36%), Postives = 575/737 (78.02%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKL F F + LAS LD SD+DG+ +YIVY+G K ED S HH MLE+V
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLD-SDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAP+++LH+YKRSFNGF VKLTEEEA+KI++ EGVVSVF N LHTTRSWDF+G
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
F +V R QVESNIVVGVLD+GIWPESPSF D G+ PPP KWKG C+TS NFRCNRKII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GAR+Y P D+ PRD++GHGTHTAST AGGLV+QA+LYGL LGTARGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
A YK+CW+DGC D DILAA+DDAIADGVDIISLSVGG P++YF D+IAIG+FH+++ GI
Sbjct: 241 AAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGN GP++FT + SPW LSVAAS++DRK V++VQ+GN +QG +INTFD +
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQ 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSP-STFASFNGAVG 420
YPL+ + PN GF S+SRFC+ SV+ NL++GKI++C++ P F S +GA G
Sbjct: 361 YYPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAG 420
Query: 421 VVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVS 480
V+M + +D + SYPLPSS L P Y+ S + P ATIFKS + + SAP++VS
Sbjct: 421 VLMT-SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVS 480
Query: 481 FSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHA 540
FSSRGPN T D++KPD++ PGVEILAAW +A V G+ R TL+NIISGTSMSCPH
Sbjct: 481 FSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGI--RRNTLFNIISGTSMSCPHI 540
Query: 541 TAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYD 600
T A YVKT++P+WSPAAIKSALMTTA P+N + NPQAEFAYG+GH+NP+KAV PGLVYD
Sbjct: 541 TGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYD 600
Query: 601 AQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFF 660
A ESDYV+FLCGQGY T VRR++GD+S CT N+GRVWDLNYPSF LS +PS++ NQ+F
Sbjct: 601 ANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYF 660
Query: 661 RRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSA 720
RT+T+V + +TYRA ++ AP+GLTISVNP LSFN +G +KSFT+TVRG + +VSA
Sbjct: 661 NRTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSA 720
Query: 721 ALLWTDGHHHVRSPITV 737
+L+W+DG H+VRSPIT+
Sbjct: 721 SLVWSDGVHYVRSPITI 727
BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 678.7 bits (1750), Expect = 7.4e-194
Identity = 353/722 (48.89%), Postives = 478/722 (66.20%), Query Frame = 0
Query: 25 DSSDNDGRK---VYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGF 84
D S ND R+ VYIVY+G PE S PSHH+ +L+++VG+ A L+ SYKRSFNGF
Sbjct: 20 DVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGF 79
Query: 85 VVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSVRRVKQVESNIVVGVLD 144
L++ E+QK+ + VVSVFP+ L TTRSWDF+GF + RR ES+++VGV+D
Sbjct: 80 AANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVID 139
Query: 145 SGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPEDIKSPRD 204
SGIWPES SF D G+GPPP KWKG+C+ F CN K+IGAR Y N F S RD
Sbjct: 140 SGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY---NKF----ADSARD 199
Query: 205 SDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFD 264
+GHGTHTAST AG V AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFD
Sbjct: 200 EEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFD 259
Query: 265 DAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSP 324
DAIADGVD+IS+S+ + N S+AIG+FH+M GI+T+ SAGN+GPD ++ N SP
Sbjct: 260 DAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSP 319
Query: 325 WSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSS 384
W ++VAAS DR+ + +V LGN G ++NTF+L G ++P++Y NVS + +
Sbjct: 320 WMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQ 379
Query: 385 SRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSYPLPSSYL 444
+ +CS VD LV+GKI+LCD L A GA+GV++ + + D++ P P+S L
Sbjct: 380 AGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSL 439
Query: 445 GPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPG 504
G +IK+Y+ S + P A I ++ + D AP + SFSSRGP+ ++LKPD++APG
Sbjct: 440 GFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPG 499
Query: 505 VEILAAWSPIASVSSGVS--DSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWSPAAIK 564
+EILAA+SP+AS SS ++ D R+ Y+++SGTSM+CPH A YVK+FHP WSP+AIK
Sbjct: 500 LEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIK 559
Query: 565 SALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMV 624
SA+MTTA P+N K NP+ EFAYG+G INP KA +PGLVY+ + DY++ LC +G+ + +
Sbjct: 560 SAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTL 619
Query: 625 RRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLG 684
SG + C+ V DLNYP+ + + N F+RTVTNVG +TY+A V+
Sbjct: 620 TTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVP 679
Query: 685 APRGLTISVNPPALSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWTDGHHHVRSPITVY 740
L IS+ P L F + +KKSF VT+ G + VS++++W+DG H VRSPI Y
Sbjct: 680 LQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 727
BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 646.4 bits (1666), Expect = 4.1e-184
Identity = 342/732 (46.72%), Postives = 479/732 (65.44%), Query Frame = 0
Query: 18 SLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKR 77
SL + S D D+ ++VYIVYLG+ P TP S HM +L+E+ G + + L+ SYK+
Sbjct: 18 SLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKK 77
Query: 78 SFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSV--RRVKQVESN 137
SFNGF +LTE E ++++ E VVSVFP+ K L TT SW+FMG + + +R + +ES+
Sbjct: 78 SFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESD 137
Query: 138 IVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPE 197
++GV+DSGI+PES SFSD G+GPPP KWKG C NF CN K+IGAR Y + + +
Sbjct: 138 TIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS----K 197
Query: 198 DIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDA 257
++ RD GHGTHTAS AG V ++ YGL GTARGGVP+ARIAVYK+C ++GC
Sbjct: 198 ANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGE 257
Query: 258 DILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYF 317
+++AFDDAIADGVD+IS+S+ + + D IAIGAFH+M G+LT N+AGN+GP
Sbjct: 258 AMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKIS 317
Query: 318 TIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVS 377
T+ + +PW SVAAS +R ++KV LG+ + G ++NT+D+ G YPL+Y +A
Sbjct: 318 TVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSA--AL 377
Query: 378 GGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSY 437
+ +R C +D LV+GKI+LCDS +V N + RS+
Sbjct: 378 STCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFIRSF 437
Query: 438 PLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILK 497
P+ S+L ++ +YM S K P AT+ KS +++ APL+ SFSSRGP+ DILK
Sbjct: 438 PV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILK 497
Query: 498 PDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWS 557
PD+TAPGVEILAA+SP +S + D+R Y+++SGTSM+CPH A YVKTFHP WS
Sbjct: 498 PDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWS 557
Query: 558 PAAIKSALMTTAIPLNPKLN--PQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQ 617
P+ I+SA+MTTA P+N + EFAYG+GH++P+ A+NPGLVY+ ++D++ FLCG
Sbjct: 558 PSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGL 617
Query: 618 GYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNVGSKVA 677
YT+ +R +SGD+S CT+ S + +LNYP+ + + ++ N F+RTVTNVG + +
Sbjct: 618 NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKS 677
Query: 678 TYRAKVLGAPRG-LTISVNPPALSFNAIGQKKSFTVTVRG---GVNQPIVSAALLWTDGH 737
TY AKV+ P L+I V+P LS ++ +K+SF VTV G QP VSA L+W+DG
Sbjct: 678 TYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP-VSANLIWSDGT 737
Query: 738 HHVRSPITVYVV 740
H+VRSPI VY +
Sbjct: 738 HNVRSPIIVYAM 740
BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 645.6 bits (1664), Expect = 6.9e-184
Identity = 354/745 (47.52%), Postives = 477/745 (64.03%), Query Frame = 0
Query: 4 SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
S IF L++ +SF+ + D+ ++VYIVY+G P P SHH +L++V G
Sbjct: 11 SCIFALLV-VSFASA-------GKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTG 70
Query: 64 STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
+ D L+ +YKRSFNGF +LTE E + +++ + VVSVFP+ +L TT SW+FMG
Sbjct: 71 ESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLK 130
Query: 124 --KSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
K +R +ES+ ++GV+DSGI+PES SFS G+GPPP KWKG C+ NF CN K+I
Sbjct: 131 EGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLI 190
Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
GAR Y PE S RD+ GHG+HTAS AG V S YGL GT RGGVP+ARI
Sbjct: 191 GARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARI 250
Query: 244 AVYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
AVYK+C C ILAAFDDAIAD VDII++S+G + + D++AIGAFH+M
Sbjct: 251 AVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAK 310
Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
GILT N AGN+GP+ TI + +PW +VAAS+++R ++KV LGN G ++N+FDL
Sbjct: 311 GILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLN 370
Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
GK+YPL+Y +A S SS+ FCS +D V+GKI+LCD+ +P +
Sbjct: 371 GKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVA 430
Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
+V N +D + + P S L N + +Y+ S K P A + KS + + AP++
Sbjct: 431 SIVRNP--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 490
Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
S+SSRGPNP +DILKPD+TAPG EILAA+SP S SD+R Y +ISGTSMSCPH
Sbjct: 491 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPH 550
Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQ---AEFAYGAGHINPVKAVNPG 603
A Y+KTFHP WSP+ I+SA+MTTA P+N +P AEFAYGAGH++P+ A++PG
Sbjct: 551 VAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPG 610
Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW-DLNYPSFALSSTPSES 663
LVY+A +SD++ FLCG YT +R +SGD S CT+ + + +LNYPS + + ++
Sbjct: 611 LVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKP 670
Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
FRRTVTNVG ATY+AKV+G+ L + V P LS ++ +KKSFTVTV G
Sbjct: 671 FKVTFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPK 730
Query: 724 NQPIVSAALLWTDGHHHVRSPITVY 738
+ +VSA L+W+DG H VRSPI VY
Sbjct: 731 AENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match:
F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)
HSP 1 Score: 636.3 bits (1640), Expect = 4.2e-181
Identity = 356/748 (47.59%), Postives = 476/748 (63.64%), Query Frame = 0
Query: 4 SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
S IF L++ +SF +S D D D ++ YIVY+G P P SHH +L++V G
Sbjct: 12 SCIFALLV-VSF------ASADKDDQD-KQEYIVYMGALPARVDYMPMSHHTSILQDVTG 71
Query: 64 STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
+ D L+ +YKRSFNGF +LT+ E + +++ + VVSVFPN K L TT SW+FMG
Sbjct: 72 ESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLK 131
Query: 124 KSVRRVKQ--VESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
+S R + +ES+ ++GV+DSGI+PES SFS G+GPPP KWKG C+ NF N K+I
Sbjct: 132 ESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLI 191
Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
GAR Y PE S RD GHG+HTAST AG V S YGL GTARGGVP+ARI
Sbjct: 192 GARYYTPKLEGFPE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARI 251
Query: 244 AVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
AVYK+C DGC ILAAFDDAIAD VDII++S+GG+ + D IAIGAFH+M
Sbjct: 252 AVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAK 311
Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
GIL NSAGN GP+ T+ + +PW +VAAS+ +R V+KV LGN G ++N+FDL
Sbjct: 312 GILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLN 371
Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
GK+YPL+Y +A + G +S+ FCS +D V+GKI+LCDS +P +
Sbjct: 372 GKKYPLVYGKSASSSCG---AASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIA 431
Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
+V + D + + P S L N + +YM S K P A + KS + + AP++
Sbjct: 432 SIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVA 491
Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
S+ SRGPN DILKPD+TAPG EI+AA+SP A S +SD+R Y++ +GTSMSCPH
Sbjct: 492 SYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPH 551
Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNP---QAEFAYGAGHINPVKAVNPG 603
A Y+K+FHP WSP+ I+SA+MTTA P+N +P AEFAYGAGH++P+ A++PG
Sbjct: 552 VAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPG 611
Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSES 663
LVY+A +SD++ FLCG YT +R +SGD S CT+ + + +LNYPS + ++
Sbjct: 612 LVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKP 671
Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
FRRTVTNVG ATY+AKV+G+ L + V P LS ++ +KKSFTVT G
Sbjct: 672 FKVIFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTASGAGPK 731
Query: 724 NQPIVSAALLWTDGHHHVRSPITVYVVD 741
+ +VSA L+W+DG H VRSPI VY +
Sbjct: 732 AENLVSAQLIWSDGVHFVRSPIVVYATN 738
BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match:
A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)
HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 742/742 (100.00%), Postives = 742/742 (100.00%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match:
A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)
HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 734/742 (98.92%), Postives = 737/742 (99.33%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKLVIFISFSGSLLAS LDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMNDAGVKDN+RSYPLPSSYLGPVAGNNIKTYMGS+KFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
QESDYVRFLCGQGYTTAMVRRLSGDHSVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match:
A0A5A7UBK2 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001150 PE=3 SV=1)
HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 662/741 (89.34%), Postives = 703/741 (94.87%), Query Frame = 0
Query: 2 SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61
SSSL+FK V+F S SLLAS LD SDNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVV
Sbjct: 3 SSSLVFKFVVFSSLFCSLLASGLD-SDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVV 62
Query: 62 GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121
GS+FAP+ALLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPN KKHLHTTRSWDFMGF
Sbjct: 63 GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 122
Query: 122 TKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181
T++V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG
Sbjct: 123 TQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 182
Query: 182 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241
ARAYRSD FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIA
Sbjct: 183 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIA 242
Query: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301
VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGIL
Sbjct: 243 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGIL 302
Query: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361
TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQ
Sbjct: 303 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQ 362
Query: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421
YPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S NGA+GVV
Sbjct: 363 YPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVV 422
Query: 422 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 481
MND GVKDN+RSYPLPSSYL PV GNNIKTYM NKFPTATI KSNAVNDTSAP IVSFS
Sbjct: 423 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 482
Query: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541
SRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV DSRTTLYNIISGTSMSCPHATA
Sbjct: 483 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 542
Query: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601
AAVYVKTFHP+WSPAAIKSALMTTA PLN KLN Q EFAYGAGHINP+KAV+PGL+YDA
Sbjct: 543 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 602
Query: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 661
E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRR
Sbjct: 603 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 662
Query: 662 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721
TVTNVGSKV+TYRAKV+GAPRGL+I+VNPP LSFNAIGQKKSFT+T+RG + Q IVSA+L
Sbjct: 663 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 722
Query: 722 LWTDGHHHVRSPITVYVVDKA 743
+W+DG+H+VRSPITV+VV A
Sbjct: 723 MWSDGYHNVRSPITVFVVGTA 742
BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match:
A0A1S3CFE1 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1)
HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 662/741 (89.34%), Postives = 703/741 (94.87%), Query Frame = 0
Query: 2 SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61
SSSL+FK V+F S SLLAS LD SDNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVV
Sbjct: 15 SSSLVFKFVVFSSLFCSLLASGLD-SDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVV 74
Query: 62 GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121
GS+FAP+ALLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPN KKHLHTTRSWDFMGF
Sbjct: 75 GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 134
Query: 122 TKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181
T++V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG
Sbjct: 135 TQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 194
Query: 182 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241
ARAYRSD FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIA
Sbjct: 195 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIA 254
Query: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301
VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGIL
Sbjct: 255 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGIL 314
Query: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361
TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQ
Sbjct: 315 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQ 374
Query: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421
YPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S NGA+GVV
Sbjct: 375 YPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVV 434
Query: 422 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 481
MND GVKDN+RSYPLPSSYL PV GNNIKTYM NKFPTATI KSNAVNDTSAP IVSFS
Sbjct: 435 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 494
Query: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541
SRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV DSRTTLYNIISGTSMSCPHATA
Sbjct: 495 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 554
Query: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601
AAVYVKTFHP+WSPAAIKSALMTTA PLN KLN Q EFAYGAGHINP+KAV+PGL+YDA
Sbjct: 555 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 614
Query: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 661
E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRR
Sbjct: 615 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 674
Query: 662 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721
TVTNVGSKV+TYRAKV+GAPRGL+I+VNPP LSFNAIGQKKSFT+T+RG + Q IVSA+L
Sbjct: 675 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 734
Query: 722 LWTDGHHHVRSPITVYVVDKA 743
+W+DG+H+VRSPITV+VV A
Sbjct: 735 MWSDGYHNVRSPITVFVVGTA 754
BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match:
A0A6J1K719 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)
HSP 1 Score: 1295.8 bits (3352), Expect = 0.0e+00
Identity = 632/742 (85.18%), Postives = 697/742 (93.94%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
M SS+IFKLV F+ F G LLASSLD SDNDGRKVYIVYLGNKP+D ASTPSHHMR+LEEV
Sbjct: 13 MGSSVIFKLV-FLGFFGCLLASSLD-SDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEV 72
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTF+PDALLHSYKRSFNGF V+LTEEEAQKI AK+GVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 73 VGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMG 132
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FT +V RV QVES+IVVGVLDSGIWPESPSFSDVGYGP PAKWKGACQ S NFRCN+KII
Sbjct: 133 FTTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKII 192
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSDN FPPED +SPRDSDGHGTHTASTVAGGLV+QASLYGLALGTARGGVPSARI
Sbjct: 193 GARAYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARI 252
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG++PKYYFNDSIAIGAFHSMKHGI
Sbjct: 253 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGI 312
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS VQLGNKN++QGYTINTFDL+GK
Sbjct: 313 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGK 372
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
YPLIYAG+APN+SGGF+GSSSR+CS+NSVDRNLV+GKIL+CDSILSPSTFAS + AVGV
Sbjct: 373 HYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGV 432
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMN+AGVKDN+RSYPLPSSYL AGN++K Y+GSN FPTATIFKSN VNDT+AP++VSF
Sbjct: 433 VMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSF 492
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAW+PIA VSSG+ DSRTTLYNIISGTSMSCPHAT
Sbjct: 493 SSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHAT 552
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNPQAEFAYGAGHI+P+KA+NPGL+YDA
Sbjct: 553 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDA 612
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
E+DYV FLCGQGY+TAMVRRL+GD SVCT ANSGRVWDLNYPSFALS+TPSESINQFF
Sbjct: 613 NETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFT 672
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RT+TNVGS+ +TY +K+LGAP GLTI+V+PP LSFN IG K+SFT+T+ G ++Q IVSA+
Sbjct: 673 RTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSAS 732
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
++W+DG H+VRSPIT+Y+V+KA
Sbjct: 733 VVWSDGSHNVRSPITIYIVNKA 752
BLAST of CmaCh04G005370 vs. NCBI nr
Match:
XP_022979186.1 (cucumisin-like [Cucurbita maxima])
HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 742/742 (100.00%), Postives = 742/742 (100.00%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of CmaCh04G005370 vs. NCBI nr
Match:
XP_022941960.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 734/742 (98.92%), Postives = 737/742 (99.33%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKLVIFISFSGSLLAS LDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMNDAGVKDN+RSYPLPSSYLGPVAGNNIKTYMGS+KFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
QESDYVRFLCGQGYTTAMVRRLSGDHSVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of CmaCh04G005370 vs. NCBI nr
Match:
KAG6600369.1 (hypothetical protein SDJN03_05602, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1471.1 bits (3807), Expect = 0.0e+00
Identity = 733/742 (98.79%), Postives = 736/742 (99.19%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKLVIFISFSG LLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGCLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMNDAGVKDN+R+YPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARTYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAWSPI SVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIVSVSSGVSDSRTTLYNIISGTSMSCPHAT 540
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
QESDYVRFLCGQGYTTAMVRRLSGDHSVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of CmaCh04G005370 vs. NCBI nr
Match:
XP_023536304.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 731/742 (98.52%), Postives = 735/742 (99.06%), Query Frame = 0
Query: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1 MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
Query: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYG PPAKWKGACQTSANFRCNRKI+
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIV 180
Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
GARAYRSD FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGV 420
Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
VMNDAGVKDN+RSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSN VNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSF 480
Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV DSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHAT 540
Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742
BLAST of CmaCh04G005370 vs. NCBI nr
Match:
XP_008461729.2 (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 662/741 (89.34%), Postives = 703/741 (94.87%), Query Frame = 0
Query: 2 SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61
SSSL+FK V+F S SLLAS LD SDNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVV
Sbjct: 15 SSSLVFKFVVFSSLFCSLLASGLD-SDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVV 74
Query: 62 GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121
GS+FAP+ALLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPN KKHLHTTRSWDFMGF
Sbjct: 75 GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 134
Query: 122 TKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181
T++V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG
Sbjct: 135 TQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 194
Query: 182 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241
ARAYRSD FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIA
Sbjct: 195 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIA 254
Query: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301
VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGIL
Sbjct: 255 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGIL 314
Query: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361
TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQ
Sbjct: 315 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQ 374
Query: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421
YPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S NGA+GVV
Sbjct: 375 YPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVV 434
Query: 422 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 481
MND GVKDN+RSYPLPSSYL PV GNNIKTYM NKFPTATI KSNAVNDTSAP IVSFS
Sbjct: 435 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 494
Query: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541
SRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV DSRTTLYNIISGTSMSCPHATA
Sbjct: 495 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 554
Query: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601
AAVYVKTFHP+WSPAAIKSALMTTA PLN KLN Q EFAYGAGHINP+KAV+PGL+YDA
Sbjct: 555 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 614
Query: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 661
E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRR
Sbjct: 615 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 674
Query: 662 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721
TVTNVGSKV+TYRAKV+GAPRGL+I+VNPP LSFNAIGQKKSFT+T+RG + Q IVSA+L
Sbjct: 675 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 734
Query: 722 LWTDGHHHVRSPITVYVVDKA 743
+W+DG+H+VRSPITV+VV A
Sbjct: 735 MWSDGYHNVRSPITVFVVGTA 754
BLAST of CmaCh04G005370 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 665.6 bits (1716), Expect = 4.6e-191
Identity = 343/705 (48.65%), Postives = 467/705 (66.24%), Query Frame = 0
Query: 39 LGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEG 98
+G PE S PSHH+ +L+++VG+ A L+ SYKRSFNGF L++ E+QK+ +
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 99 VVSVFPNGKKHLHTTRSWDFMGFTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 158
VVSVFP+ L TTRSWDF+GF + RR ES+++VGV+DSGIWPES SF D G+GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 159 PPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLV 218
PP KWKG+C+ F CN K+IGAR Y N F S RD +GHGTHTAST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY---NKF----ADSARDEEGHGTHTASTAAGNAV 180
Query: 219 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 278
AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+ +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240
Query: 279 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSK 338
N S+AIG+FH+M GI+T+ SAGN+GPD ++ N SPW ++VAAS DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300
Query: 339 VQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGK 398
V LGN G ++NTF+L G ++P++Y NVS + + + +CS VD LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360
Query: 399 ILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKF 458
I+LCD L A GA+GV++ + + D++ P P+S LG +IK+Y+ S +
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420
Query: 459 PTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV 518
P A I ++ + D AP + SFSSRGP+ ++LKPD++APG+EILAA+SP+AS SS +
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480
Query: 519 S--DSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQ 578
+ D R+ Y+++SGTSM+CPH A YVK+FHP WSP+AIKSA+MTTA P+N K NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540
Query: 579 AEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGR 638
EFAYG+G INP KA +PGLVY+ + DY++ LC +G+ + + SG + C+
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600
Query: 639 VWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFN 698
V DLNYP+ + + N F+RTVTNVG +TY+A V+ L IS+ P L F
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660
Query: 699 AIGQKKSFTVTVRGG--VNQPIVSAALLWTDGHHHVRSPITVYVV 740
+ +KKSF VT+ G + VS++++W+DG H VRSPI Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691
BLAST of CmaCh04G005370 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 646.4 bits (1666), Expect = 2.9e-185
Identity = 342/732 (46.72%), Postives = 479/732 (65.44%), Query Frame = 0
Query: 18 SLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKR 77
SL + S D D+ ++VYIVYLG+ P TP S HM +L+E+ G + + L+ SYK+
Sbjct: 18 SLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKK 77
Query: 78 SFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSV--RRVKQVESN 137
SFNGF +LTE E ++++ E VVSVFP+ K L TT SW+FMG + + +R + +ES+
Sbjct: 78 SFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESD 137
Query: 138 IVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPE 197
++GV+DSGI+PES SFSD G+GPPP KWKG C NF CN K+IGAR Y + + +
Sbjct: 138 TIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS----K 197
Query: 198 DIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDA 257
++ RD GHGTHTAS AG V ++ YGL GTARGGVP+ARIAVYK+C ++GC
Sbjct: 198 ANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGE 257
Query: 258 DILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYF 317
+++AFDDAIADGVD+IS+S+ + + D IAIGAFH+M G+LT N+AGN+GP
Sbjct: 258 AMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKIS 317
Query: 318 TIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVS 377
T+ + +PW SVAAS +R ++KV LG+ + G ++NT+D+ G YPL+Y +A
Sbjct: 318 TVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSA--AL 377
Query: 378 GGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSY 437
+ +R C +D LV+GKI+LCDS +V N + RS+
Sbjct: 378 STCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFIRSF 437
Query: 438 PLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILK 497
P+ S+L ++ +YM S K P AT+ KS +++ APL+ SFSSRGP+ DILK
Sbjct: 438 PV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILK 497
Query: 498 PDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWS 557
PD+TAPGVEILAA+SP +S + D+R Y+++SGTSM+CPH A YVKTFHP WS
Sbjct: 498 PDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWS 557
Query: 558 PAAIKSALMTTAIPLNPKLN--PQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQ 617
P+ I+SA+MTTA P+N + EFAYG+GH++P+ A+NPGLVY+ ++D++ FLCG
Sbjct: 558 PSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGL 617
Query: 618 GYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNVGSKVA 677
YT+ +R +SGD+S CT+ S + +LNYP+ + + ++ N F+RTVTNVG + +
Sbjct: 618 NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKS 677
Query: 678 TYRAKVLGAPRG-LTISVNPPALSFNAIGQKKSFTVTVRG---GVNQPIVSAALLWTDGH 737
TY AKV+ P L+I V+P LS ++ +K+SF VTV G QP VSA L+W+DG
Sbjct: 678 TYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP-VSANLIWSDGT 737
Query: 738 HHVRSPITVYVV 740
H+VRSPI VY +
Sbjct: 738 HNVRSPIIVYAM 740
BLAST of CmaCh04G005370 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 645.6 bits (1664), Expect = 4.9e-185
Identity = 354/745 (47.52%), Postives = 477/745 (64.03%), Query Frame = 0
Query: 4 SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
S IF L++ +SF+ + D+ ++VYIVY+G P P SHH +L++V G
Sbjct: 11 SCIFALLV-VSFASA-------GKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTG 70
Query: 64 STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
+ D L+ +YKRSFNGF +LTE E + +++ + VVSVFP+ +L TT SW+FMG
Sbjct: 71 ESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLK 130
Query: 124 --KSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
K +R +ES+ ++GV+DSGI+PES SFS G+GPPP KWKG C+ NF CN K+I
Sbjct: 131 EGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLI 190
Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
GAR Y PE S RD+ GHG+HTAS AG V S YGL GT RGGVP+ARI
Sbjct: 191 GARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARI 250
Query: 244 AVYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
AVYK+C C ILAAFDDAIAD VDII++S+G + + D++AIGAFH+M
Sbjct: 251 AVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAK 310
Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
GILT N AGN+GP+ TI + +PW +VAAS+++R ++KV LGN G ++N+FDL
Sbjct: 311 GILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLN 370
Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
GK+YPL+Y +A S SS+ FCS +D V+GKI+LCD+ +P +
Sbjct: 371 GKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVA 430
Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
+V N +D + + P S L N + +Y+ S K P A + KS + + AP++
Sbjct: 431 SIVRNP--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 490
Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
S+SSRGPNP +DILKPD+TAPG EILAA+SP S SD+R Y +ISGTSMSCPH
Sbjct: 491 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPH 550
Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQ---AEFAYGAGHINPVKAVNPG 603
A Y+KTFHP WSP+ I+SA+MTTA P+N +P AEFAYGAGH++P+ A++PG
Sbjct: 551 VAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPG 610
Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW-DLNYPSFALSSTPSES 663
LVY+A +SD++ FLCG YT +R +SGD S CT+ + + +LNYPS + + ++
Sbjct: 611 LVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKP 670
Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
FRRTVTNVG ATY+AKV+G+ L + V P LS ++ +KKSFTVTV G
Sbjct: 671 FKVTFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPK 730
Query: 724 NQPIVSAALLWTDGHHHVRSPITVY 738
+ +VSA L+W+DG H VRSPI VY
Sbjct: 731 AENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of CmaCh04G005370 vs. TAIR 10
Match:
AT3G46840.1 (Subtilase family protein )
HSP 1 Score: 636.3 bits (1640), Expect = 3.0e-182
Identity = 356/748 (47.59%), Postives = 476/748 (63.64%), Query Frame = 0
Query: 4 SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
S IF L++ +SF +S D D D ++ YIVY+G P P SHH +L++V G
Sbjct: 12 SCIFALLV-VSF------ASADKDDQD-KQEYIVYMGALPARVDYMPMSHHTSILQDVTG 71
Query: 64 STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
+ D L+ +YKRSFNGF +LT+ E + +++ + VVSVFPN K L TT SW+FMG
Sbjct: 72 ESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLK 131
Query: 124 KSVRRVKQ--VESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
+S R + +ES+ ++GV+DSGI+PES SFS G+GPPP KWKG C+ NF N K+I
Sbjct: 132 ESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLI 191
Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
GAR Y PE S RD GHG+HTAST AG V S YGL GTARGGVP+ARI
Sbjct: 192 GARYYTPKLEGFPE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARI 251
Query: 244 AVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
AVYK+C DGC ILAAFDDAIAD VDII++S+GG+ + D IAIGAFH+M
Sbjct: 252 AVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAK 311
Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
GIL NSAGN GP+ T+ + +PW +VAAS+ +R V+KV LGN G ++N+FDL
Sbjct: 312 GILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLN 371
Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
GK+YPL+Y +A + G +S+ FCS +D V+GKI+LCDS +P +
Sbjct: 372 GKKYPLVYGKSASSSCG---AASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIA 431
Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
+V + D + + P S L N + +YM S K P A + KS + + AP++
Sbjct: 432 SIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVA 491
Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
S+ SRGPN DILKPD+TAPG EI+AA+SP A S +SD+R Y++ +GTSMSCPH
Sbjct: 492 SYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPH 551
Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNP---QAEFAYGAGHINPVKAVNPG 603
A Y+K+FHP WSP+ I+SA+MTTA P+N +P AEFAYGAGH++P+ A++PG
Sbjct: 552 VAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPG 611
Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSES 663
LVY+A +SD++ FLCG YT +R +SGD S CT+ + + +LNYPS + ++
Sbjct: 612 LVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKP 671
Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
FRRTVTNVG ATY+AKV+G+ L + V P LS ++ +KKSFTVT G
Sbjct: 672 FKVIFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTASGAGPK 731
Query: 724 NQPIVSAALLWTDGHHHVRSPITVYVVD 741
+ +VSA L+W+DG H VRSPI VY +
Sbjct: 732 AENLVSAQLIWSDGVHFVRSPIVVYATN 738
BLAST of CmaCh04G005370 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 629.4 bits (1622), Expect = 3.7e-180
Identity = 348/748 (46.52%), Postives = 470/748 (62.83%), Query Frame = 0
Query: 2 SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEV 61
SSSL+ L++ L SS+ S+ D ++VYIVY+G+ + TP S HM +L+EV
Sbjct: 7 SSSLLSCLLV-------LFLSSV-SAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEV 66
Query: 62 VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 121
G + L+ SYKRSFNGF +LTE E ++++ GVVSVFPN K L TT SWDFMG
Sbjct: 67 TGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 126
Query: 122 FTKSV--RRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRK 181
+ + +R VES+ ++GV+DSGI PES SFSD G+GPPP KWKG C NF CN K
Sbjct: 127 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNK 186
Query: 182 IIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSA 241
+IGAR Y S+ RD DGHGTHTAST AG V AS +G+ GT RGGVP++
Sbjct: 187 LIGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 246
Query: 242 RIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 301
R+A YK+C GC +L+AFDDAIADGVD+I++S+G + ND IAIGAFH+M
Sbjct: 247 RVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 306
Query: 302 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 361
G+LT NSAGN GP ++ +PW L+VAAS+ +R V+KV LGN G ++N +++K
Sbjct: 307 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 366
Query: 362 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 421
GK YPL+Y +A S S+ C + VD++ V+GKIL+C S GAV
Sbjct: 367 GKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAV 426
Query: 422 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 481
G++ D + +PLP++ L ++ +Y+ S P A + K+ A+ + ++P+I
Sbjct: 427 GLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 486
Query: 482 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 541
SFSSRGPN DILKPD+TAPGVEILAA+SP S D+R Y+++SGTSMSCPH
Sbjct: 487 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPH 546
Query: 542 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLN--PQAEFAYGAGHINPVKAVNPGL 601
A YVKTF+P WSP+ I+SA+MTTA P+N EFAYG+GH++P+ A NPGL
Sbjct: 547 VAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGL 606
Query: 602 VYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSF-ALSSTPSESI 661
VY+ +SD++ FLCG YT+ +++ +SG+ C+ A +LNYPS A S +
Sbjct: 607 VYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTF 666
Query: 662 NQFFRRTVTNVGSKVATYRAKVL-GAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQ 721
F RT+TNVG+ +TY +KV+ G L + + P LSF + +K+SFTVTV G
Sbjct: 667 TVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLD 726
Query: 722 PIV--SAALLWTDGHHHVRSPITVYVVD 741
V SA L+W+DG H+VRSPI VY D
Sbjct: 727 SEVPSSANLIWSDGTHNVRSPIVVYTSD 731
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q39547 | 2.8e-270 | 63.36 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
Q9FIF8 | 7.4e-194 | 48.89 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
Q9FGU3 | 4.1e-184 | 46.72 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
Q9STF7 | 6.9e-184 | 47.52 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
F4JA91 | 4.2e-181 | 47.59 | Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1IQ27 | 0.0e+00 | 100.00 | cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1 | [more] |
A0A6J1FV97 | 0.0e+00 | 98.92 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1 | [more] |
A0A5A7UBK2 | 0.0e+00 | 89.34 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00115... | [more] |
A0A1S3CFE1 | 0.0e+00 | 89.34 | cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1 | [more] |
A0A6J1K719 | 0.0e+00 | 85.18 | cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1 | [more] |