CmaCh04G005370 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G005370
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncucumisin-like
LocationCma_Chr04: 2728452 .. 2732737 (-)
RNA-Seq ExpressionCmaCh04G005370
SyntenyCmaCh04G005370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCCTCTCTGATCTTCAAGCTGGTTATCTTCATCAGCTTCTCGGGTAGCCTGCTCGCTTCTAGCCTTGATTCTTCCGACAACGATGGCCGAAAGGTATAAACGACCGACCTTCATTTCCCGATCGATCGGATTCAACGTTCCAGATTTTAAAGCGTTTGTTCTCTTTAATTTGCAGGTTTATATTGTGTACTTGGGGAACAAGCCTGAGGATTCAGCTTCCACTCCTTCACATCATATGAGAATGTTGGAGGAAGTGGTTGGCAGGTTCATAATCTTTCATTGATTTTTATCACAATTTCTATGAAAACATGCACTCATTTTTGTAAATTTGTTTGTTCTTAGCACATTCGCTCCCGACGCGCTACTCCATAGCTATAAGAGAAGTTTCAATGGATTCGTCGTTAAGCTCACTGAAGAAGAAGCTCAAAAGATTTCGGGTACGTATGATTATTCAAACCCCATTAAATTTGTAGCTTAAGACTGTTCGTCGATTGAATTGAGATATTTTTTAACTAAATCTAATGGTTCAAGTTAAATTTTTTCAACCCGAGTTAAATAATGAACCCAATTATAAAAATTTTGAATTTTGTTGGTTCGGGTTATTTAAATAATTTATTCAAATTTAATATAAGTTCGTAAAACATCTATTTATTTATTTTTAAATATTTAATTAAGATTTGGGTTAATATCCGTATATATTTTTAAAAAATAACCTTCTAAATAATTAAAAATTATTTTTGAGAGTGGTCTAATTAATTAATAAATAAATAAATATAATTGTAGGGTAAGAAAAATGTATTTTTTAAATTAATAATAAATTTCTATCCATTAATTCAATCTAATCAGTTTCAGTATAATTTAAATAAAACGGTACATTTTAGGTTAAGTTGATCAAATTTTTCGAGTGATCGAACTCTTATAGTTTAGCTCATCCATTTAGCATTTTAAGGTCATGTTCCAAACTGAGTGCATAATTTGGAATGTTTCAGCTAAGGAGGGTGTTGTCTCGGTGTTTCCAAATGGAAAGAAACATCTTCACACGACAAGATCATGGGATTTCATGGGTTTTACTAAAAGTGTTCGTCGCGTAAAACAAGTCGAAAGCAACATAGTCGTCGGAGTTTTGGACAGTGGAATCTGGCCAGAGTCTCCTAGCTTCAGCGACGTTGGTTATGGCCCTCCACCAGCCAAATGGAAGGGCGCTTGCCAAACCTCCGCCAACTTTCGCTGCAACAGGTAACATTAAAAAAAGTTATTATCGTCAGTATATGAATAAAAACCGTCTATTTCTATAAAGTAATAACATTCAAATGGTTTGAACTAACTATCAGAAAAATCATTGGAGCTCGAGCATACCGTAGTGACAACTTGTTTCCTCCCGAAGACATCAAAAGTCCAAGAGATTCAGACGGTCATGGGACACACACTGCATCGACCGTGGCAGGTGGTCTCGTGAACCAAGCAAGTTTATATGGTCTTGCGCTTGGCACAGCTAGAGGAGGGGTCCCGTCTGCGCGCATTGCTGTGTATAAGATATGTTGGTCCGATGGGTGCTACGATGCCGATATTCTTGCAGCGTTCGACGACGCAATCGCGGACGGTGTCGATATCATATCGCTATCGGTTGGGGGGAACAAGCCCAAGTATTACTTCAATGATTCAATTGCCATTGGAGCTTTCCACTCCATGAAGCATGGGATATTGACATCCAACTCCGCCGGGAACGACGGCCCTGACTACTTCACCATCAGGAATTTCTCTCCATGGTCTCTTTCTGTGGCTGCAAGCTCCATTGATAGAAAGTTGGTATCAAAAGTGCAGCTTGGCAACAAAAATGTCTATCAGGTGATACTTTTTTCCGTCGTACGAGTATCGATCTTTCGAACATTAATTTGAAATGATATCTCACGAACATACTCAAATTTTGGACGCAGGGATATACAATTAACACGTTTGATCTTAAAGGAAAACAATATCCGCTAATTTATGCTGGAAATGCACCCAATGTTTCTGGTGGCTTCACTGGCTCGAGCTCCAGGTAATTAACCATATGGGACCAAAGATGTCCATGGGGTGGAGCGGGGATAGGATCCCCGTCTCTGCCTCCTATTTCAATCCTTGTTCATGTGAAATTTCTTATTTATTTTTATGGAGATTTCCTATAGAGAATTCGTGGGGATCGGATTCCCCATATGAAGTGTTCGGGGTGGGTTGGGCAGGGACAAAAACAATGAATATAATCCCGTCCTCAGCCCCATTTAGCTTATGGGAAAAAATCTCACTCCCGCTCTCTCCCCCATTTCTGGTTTAAACAGAAATTTCTCATTCAGTTTGGGACAGATCACACCTTTTTGCATAGACGTCTCTAATTTTAGGGTTTATTTTACTAACATTTTTCTTACATTATTCGAACAGATTTTGTTCGAGAAACTCAGTAGATCGCAATTTGGTGAGGGGAAAAATCCTTCTTTGTGATTCCATATTGTCCCCTTCAACATTTGCTTCCTTCAATGGCGCGGTTGGCGTCGTTATGAATGATGCTGGCGTGAAAGACAATTCAAGATCATATCCCTTACCTTCTTCCTACCTCGGCCCGGTGGCTGGTAACAACATCAAAACCTACATGGGTTCAAACAAGTACGTTTCGACATGTTCATAAATTTAAGATCTCGATATCCTTTGTTTAAATATTAACTCCGCTTGATTTGTGAATCGATAGATTTCCAACAGCAACCATTTTCAAGAGTAACGCAGTGAACGATACATCTGCTCCTTTAATAGTTTCCTTTTCCTCCCGAGGACCCAATCCCGAAACCTACGACATTCTCAAGGTTAAACCTTTTTAGTGTAGTTTCAGATTTGAATGTATATATGGTTTTGTTAAGATGCTTAGTTTTAGTGAATTTGTATGCATGCAGCCGGATTTGACTGCCCCAGGAGTCGAAATTCTCGCAGCATGGTCACCCATTGCGTCGGTCTCTAGCGGAGTTAGCGATTCGAGGACGACGCTCTACAATATAATTTCAGGGACGTCGATGTCTTGCCCACATGCTACTGCAGCTGCTGTGTATGTCAAAACATTCCACCCTTCATGGTCTCCTGCTGCCATCAAATCAGCTCTCATGACAACAGGTAATGACAACAAAGTCATATGTTTTTTTTACATAAAACTTTCAAATGTTTTAATAAGGCTTTTAAAATTTTTAAAAAGTTTCAAAAATACTGATTTTTTAAAATTACATTAATACCCTTTAACTTATTATTAAAAAAAATAAAAAAGTTATTACTATTACACAATCATCTCTTCAAGATAAACTTATAGAATTTTTTAATTTTTTTAAAAAAATTAGATATTTTTGAAGTTTGGTATTAATAAAATTAATTGCTTCTTTGGAGTATTTTTTAAATACTCTTAAAAATTCAAATAAAATTTTGATTTTTTTTAAAATTGGTATAAAATATGAATTAAAGGTATCATTTAAAAATTAAAAATTTAAAGATATTTTTAAATTAATTTTTTTTTTTTAACTCGGGTGGATTTACTTTCTACAGCTATTCCGTTGAACCCGAAGCTGAACCCGCAAGCCGAGTTCGCGTACGGCGCAGGCCACATCAACCCAGTGAAAGCAGTAAACCCAGGGTTGGTGTACGACGCACAGGAAAGCGACTACGTTAGGTTCTTGTGTGGTCAAGGCTACACCACTGCCATGGTCCGCCGTCTCTCCGGCGACCATAGCGTCTGCACTCGCGCCAACTCCGGCCGAGTTTGGGACCTCAACTACCCTTCCTTCGCACTTTCTTCCACCCCATCAGAATCCATCAACCAATTCTTCAGAAGAACCGTCACAAACGTTGGGTCAAAGGTAGCCACATACAGAGCCAAGGTCCTTGGCGCCCCGCGTGGCCTGACCATCTCAGTCAACCCTCCTGCTCTTTCCTTCAATGCCATTGGTCAGAAGAAGTCCTTCACGGTGACGGTTCGTGGCGGTGTCAACCAACCCATTGTCTCTGCTGCTCTGTTGTGGACCGATGGGCATCACCATGTGAGAAGCCCCATCACCGTATATGTTGTGGATAAAGCTTAAATTATTATAATTATATTGTTTTTATGTGTTTGTTATGTTATTTATTTACAATTGGTTTAAAAAACTCTGTTATTTAAAAAATTGTAATATTATTGTGATCAACAATTCTCTTATTTCAAAAATTGTAATATTATCATATTAAACGCAACTCTTTCTGGAATTTTGTTATTCTCAATAATAATCCTCCGTAACAAA

mRNA sequence

ATGTCTTCCTCTCTGATCTTCAAGCTGGTTATCTTCATCAGCTTCTCGGGTAGCCTGCTCGCTTCTAGCCTTGATTCTTCCGACAACGATGGCCGAAAGGTTTATATTGTGTACTTGGGGAACAAGCCTGAGGATTCAGCTTCCACTCCTTCACATCATATGAGAATGTTGGAGGAAGTGGTTGGCAGCACATTCGCTCCCGACGCGCTACTCCATAGCTATAAGAGAAGTTTCAATGGATTCGTCGTTAAGCTCACTGAAGAAGAAGCTCAAAAGATTTCGGCTAAGGAGGGTGTTGTCTCGGTGTTTCCAAATGGAAAGAAACATCTTCACACGACAAGATCATGGGATTTCATGGGTTTTACTAAAAGTGTTCGTCGCGTAAAACAAGTCGAAAGCAACATAGTCGTCGGAGTTTTGGACAGTGGAATCTGGCCAGAGTCTCCTAGCTTCAGCGACGTTGGTTATGGCCCTCCACCAGCCAAATGGAAGGGCGCTTGCCAAACCTCCGCCAACTTTCGCTGCAACAGAAAAATCATTGGAGCTCGAGCATACCGTAGTGACAACTTGTTTCCTCCCGAAGACATCAAAAGTCCAAGAGATTCAGACGGTCATGGGACACACACTGCATCGACCGTGGCAGGTGGTCTCGTGAACCAAGCAAGTTTATATGGTCTTGCGCTTGGCACAGCTAGAGGAGGGGTCCCGTCTGCGCGCATTGCTGTGTATAAGATATGTTGGTCCGATGGGTGCTACGATGCCGATATTCTTGCAGCGTTCGACGACGCAATCGCGGACGGTGTCGATATCATATCGCTATCGGTTGGGGGGAACAAGCCCAAGTATTACTTCAATGATTCAATTGCCATTGGAGCTTTCCACTCCATGAAGCATGGGATATTGACATCCAACTCCGCCGGGAACGACGGCCCTGACTACTTCACCATCAGGAATTTCTCTCCATGGTCTCTTTCTGTGGCTGCAAGCTCCATTGATAGAAAGTTGGTATCAAAAGTGCAGCTTGGCAACAAAAATGTCTATCAGGGATATACAATTAACACGTTTGATCTTAAAGGAAAACAATATCCGCTAATTTATGCTGGAAATGCACCCAATGTTTCTGGTGGCTTCACTGGCTCGAGCTCCAGATTTTGTTCGAGAAACTCAGTAGATCGCAATTTGGTGAGGGGAAAAATCCTTCTTTGTGATTCCATATTGTCCCCTTCAACATTTGCTTCCTTCAATGGCGCGGTTGGCGTCGTTATGAATGATGCTGGCGTGAAAGACAATTCAAGATCATATCCCTTACCTTCTTCCTACCTCGGCCCGGTGGCTGGTAACAACATCAAAACCTACATGGGTTCAAACAAATTTCCAACAGCAACCATTTTCAAGAGTAACGCAGTGAACGATACATCTGCTCCTTTAATAGTTTCCTTTTCCTCCCGAGGACCCAATCCCGAAACCTACGACATTCTCAAGCCGGATTTGACTGCCCCAGGAGTCGAAATTCTCGCAGCATGGTCACCCATTGCGTCGGTCTCTAGCGGAGTTAGCGATTCGAGGACGACGCTCTACAATATAATTTCAGGGACGTCGATGTCTTGCCCACATGCTACTGCAGCTGCTGTGTATGTCAAAACATTCCACCCTTCATGGTCTCCTGCTGCCATCAAATCAGCTCTCATGACAACAGCTATTCCGTTGAACCCGAAGCTGAACCCGCAAGCCGAGTTCGCGTACGGCGCAGGCCACATCAACCCAGTGAAAGCAGTAAACCCAGGGTTGGTGTACGACGCACAGGAAAGCGACTACGTTAGGTTCTTGTGTGGTCAAGGCTACACCACTGCCATGGTCCGCCGTCTCTCCGGCGACCATAGCGTCTGCACTCGCGCCAACTCCGGCCGAGTTTGGGACCTCAACTACCCTTCCTTCGCACTTTCTTCCACCCCATCAGAATCCATCAACCAATTCTTCAGAAGAACCGTCACAAACGTTGGGTCAAAGGTAGCCACATACAGAGCCAAGGTCCTTGGCGCCCCGCGTGGCCTGACCATCTCAGTCAACCCTCCTGCTCTTTCCTTCAATGCCATTGGTCAGAAGAAGTCCTTCACGGTGACGGTTCGTGGCGGTGTCAACCAACCCATTGTCTCTGCTGCTCTGTTGTGGACCGATGGGCATCACCATGTGAGAAGCCCCATCACCGTATATGTTGTGGATAAAGCTTAAATTATTATAATTATATTGTTTTTATGTGTTTGTTATGTTATTTATTTACAATTGGTTTAAAAAACTCTGTTATTTAAAAAATTGTAATATTATTGTGATCAACAATTCTCTTATTTCAAAAATTGTAATATTATCATATTAAACGCAACTCTTTCTGGAATTTTGTTATTCTCAATAATAATCCTCCGTAACAAA

Coding sequence (CDS)

ATGTCTTCCTCTCTGATCTTCAAGCTGGTTATCTTCATCAGCTTCTCGGGTAGCCTGCTCGCTTCTAGCCTTGATTCTTCCGACAACGATGGCCGAAAGGTTTATATTGTGTACTTGGGGAACAAGCCTGAGGATTCAGCTTCCACTCCTTCACATCATATGAGAATGTTGGAGGAAGTGGTTGGCAGCACATTCGCTCCCGACGCGCTACTCCATAGCTATAAGAGAAGTTTCAATGGATTCGTCGTTAAGCTCACTGAAGAAGAAGCTCAAAAGATTTCGGCTAAGGAGGGTGTTGTCTCGGTGTTTCCAAATGGAAAGAAACATCTTCACACGACAAGATCATGGGATTTCATGGGTTTTACTAAAAGTGTTCGTCGCGTAAAACAAGTCGAAAGCAACATAGTCGTCGGAGTTTTGGACAGTGGAATCTGGCCAGAGTCTCCTAGCTTCAGCGACGTTGGTTATGGCCCTCCACCAGCCAAATGGAAGGGCGCTTGCCAAACCTCCGCCAACTTTCGCTGCAACAGAAAAATCATTGGAGCTCGAGCATACCGTAGTGACAACTTGTTTCCTCCCGAAGACATCAAAAGTCCAAGAGATTCAGACGGTCATGGGACACACACTGCATCGACCGTGGCAGGTGGTCTCGTGAACCAAGCAAGTTTATATGGTCTTGCGCTTGGCACAGCTAGAGGAGGGGTCCCGTCTGCGCGCATTGCTGTGTATAAGATATGTTGGTCCGATGGGTGCTACGATGCCGATATTCTTGCAGCGTTCGACGACGCAATCGCGGACGGTGTCGATATCATATCGCTATCGGTTGGGGGGAACAAGCCCAAGTATTACTTCAATGATTCAATTGCCATTGGAGCTTTCCACTCCATGAAGCATGGGATATTGACATCCAACTCCGCCGGGAACGACGGCCCTGACTACTTCACCATCAGGAATTTCTCTCCATGGTCTCTTTCTGTGGCTGCAAGCTCCATTGATAGAAAGTTGGTATCAAAAGTGCAGCTTGGCAACAAAAATGTCTATCAGGGATATACAATTAACACGTTTGATCTTAAAGGAAAACAATATCCGCTAATTTATGCTGGAAATGCACCCAATGTTTCTGGTGGCTTCACTGGCTCGAGCTCCAGATTTTGTTCGAGAAACTCAGTAGATCGCAATTTGGTGAGGGGAAAAATCCTTCTTTGTGATTCCATATTGTCCCCTTCAACATTTGCTTCCTTCAATGGCGCGGTTGGCGTCGTTATGAATGATGCTGGCGTGAAAGACAATTCAAGATCATATCCCTTACCTTCTTCCTACCTCGGCCCGGTGGCTGGTAACAACATCAAAACCTACATGGGTTCAAACAAATTTCCAACAGCAACCATTTTCAAGAGTAACGCAGTGAACGATACATCTGCTCCTTTAATAGTTTCCTTTTCCTCCCGAGGACCCAATCCCGAAACCTACGACATTCTCAAGCCGGATTTGACTGCCCCAGGAGTCGAAATTCTCGCAGCATGGTCACCCATTGCGTCGGTCTCTAGCGGAGTTAGCGATTCGAGGACGACGCTCTACAATATAATTTCAGGGACGTCGATGTCTTGCCCACATGCTACTGCAGCTGCTGTGTATGTCAAAACATTCCACCCTTCATGGTCTCCTGCTGCCATCAAATCAGCTCTCATGACAACAGCTATTCCGTTGAACCCGAAGCTGAACCCGCAAGCCGAGTTCGCGTACGGCGCAGGCCACATCAACCCAGTGAAAGCAGTAAACCCAGGGTTGGTGTACGACGCACAGGAAAGCGACTACGTTAGGTTCTTGTGTGGTCAAGGCTACACCACTGCCATGGTCCGCCGTCTCTCCGGCGACCATAGCGTCTGCACTCGCGCCAACTCCGGCCGAGTTTGGGACCTCAACTACCCTTCCTTCGCACTTTCTTCCACCCCATCAGAATCCATCAACCAATTCTTCAGAAGAACCGTCACAAACGTTGGGTCAAAGGTAGCCACATACAGAGCCAAGGTCCTTGGCGCCCCGCGTGGCCTGACCATCTCAGTCAACCCTCCTGCTCTTTCCTTCAATGCCATTGGTCAGAAGAAGTCCTTCACGGTGACGGTTCGTGGCGGTGTCAACCAACCCATTGTCTCTGCTGCTCTGTTGTGGACCGATGGGCATCACCATGTGAGAAGCCCCATCACCGTATATGTTGTGGATAAAGCTTAA

Protein sequence

MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAALLWTDGHHHVRSPITVYVVDKA
Homology
BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 2.8e-270
Identity = 467/737 (63.36%), Postives = 575/737 (78.02%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKL  F  F  + LAS LD SD+DG+ +YIVY+G K ED  S   HH  MLE+V
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLD-SDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAP+++LH+YKRSFNGF VKLTEEEA+KI++ EGVVSVF N    LHTTRSWDF+G
Sbjct: 61  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           F  +V R  QVESNIVVGVLD+GIWPESPSF D G+ PPP KWKG C+TS NFRCNRKII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GAR+Y       P D+  PRD++GHGTHTAST AGGLV+QA+LYGL LGTARGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           A YK+CW+DGC D DILAA+DDAIADGVDIISLSVGG  P++YF D+IAIG+FH+++ GI
Sbjct: 241 AAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGN GP++FT  + SPW LSVAAS++DRK V++VQ+GN   +QG +INTFD   +
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQ 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSP-STFASFNGAVG 420
            YPL+   + PN   GF  S+SRFC+  SV+ NL++GKI++C++   P   F S +GA G
Sbjct: 361 YYPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAG 420

Query: 421 VVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVS 480
           V+M  +  +D + SYPLPSS L P        Y+ S + P ATIFKS  + + SAP++VS
Sbjct: 421 VLMT-SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVS 480

Query: 481 FSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHA 540
           FSSRGPN  T D++KPD++ PGVEILAAW  +A V  G+   R TL+NIISGTSMSCPH 
Sbjct: 481 FSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGI--RRNTLFNIISGTSMSCPHI 540

Query: 541 TAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYD 600
           T  A YVKT++P+WSPAAIKSALMTTA P+N + NPQAEFAYG+GH+NP+KAV PGLVYD
Sbjct: 541 TGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYD 600

Query: 601 AQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFF 660
           A ESDYV+FLCGQGY T  VRR++GD+S CT  N+GRVWDLNYPSF LS +PS++ NQ+F
Sbjct: 601 ANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYF 660

Query: 661 RRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSA 720
            RT+T+V  + +TYRA ++ AP+GLTISVNP  LSFN +G +KSFT+TVRG +   +VSA
Sbjct: 661 NRTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSA 720

Query: 721 ALLWTDGHHHVRSPITV 737
           +L+W+DG H+VRSPIT+
Sbjct: 721 SLVWSDGVHYVRSPITI 727

BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 7.4e-194
Identity = 353/722 (48.89%), Postives = 478/722 (66.20%), Query Frame = 0

Query: 25  DSSDNDGRK---VYIVYLGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGF 84
           D S ND R+   VYIVY+G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF
Sbjct: 20  DVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGF 79

Query: 85  VVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSVRRVKQVESNIVVGVLD 144
              L++ E+QK+   + VVSVFP+    L TTRSWDF+GF +  RR    ES+++VGV+D
Sbjct: 80  AANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVID 139

Query: 145 SGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPEDIKSPRD 204
           SGIWPES SF D G+GPPP KWKG+C+    F CN K+IGAR Y   N F      S RD
Sbjct: 140 SGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY---NKF----ADSARD 199

Query: 205 SDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFD 264
            +GHGTHTAST AG  V  AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFD
Sbjct: 200 EEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFD 259

Query: 265 DAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSP 324
           DAIADGVD+IS+S+  +      N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SP
Sbjct: 260 DAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSP 319

Query: 325 WSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSS 384
           W ++VAAS  DR+ + +V LGN     G ++NTF+L G ++P++Y     NVS   + + 
Sbjct: 320 WMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQ 379

Query: 385 SRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSYPLPSSYL 444
           + +CS   VD  LV+GKI+LCD  L     A   GA+GV++ +  + D++   P P+S L
Sbjct: 380 AGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSL 439

Query: 445 GPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPG 504
           G     +IK+Y+ S + P A I ++  + D  AP + SFSSRGP+    ++LKPD++APG
Sbjct: 440 GFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPG 499

Query: 505 VEILAAWSPIASVSSGVS--DSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWSPAAIK 564
           +EILAA+SP+AS SS ++  D R+  Y+++SGTSM+CPH    A YVK+FHP WSP+AIK
Sbjct: 500 LEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIK 559

Query: 565 SALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMV 624
           SA+MTTA P+N K NP+ EFAYG+G INP KA +PGLVY+ +  DY++ LC +G+ +  +
Sbjct: 560 SAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTL 619

Query: 625 RRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLG 684
              SG +  C+      V DLNYP+     +  +  N  F+RTVTNVG   +TY+A V+ 
Sbjct: 620 TTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVP 679

Query: 685 APRGLTISVNPPALSFNAIGQKKSFTVTVRGG--VNQPIVSAALLWTDGHHHVRSPITVY 740
               L IS+ P  L F  + +KKSF VT+ G    +   VS++++W+DG H VRSPI  Y
Sbjct: 680 LQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 727

BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 4.1e-184
Identity = 342/732 (46.72%), Postives = 479/732 (65.44%), Query Frame = 0

Query: 18  SLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKR 77
           SL + S D  D+  ++VYIVYLG+ P     TP S HM +L+E+ G +   + L+ SYK+
Sbjct: 18  SLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKK 77

Query: 78  SFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSV--RRVKQVESN 137
           SFNGF  +LTE E ++++  E VVSVFP+ K  L TT SW+FMG  + +  +R + +ES+
Sbjct: 78  SFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESD 137

Query: 138 IVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPE 197
            ++GV+DSGI+PES SFSD G+GPPP KWKG C    NF CN K+IGAR Y + +    +
Sbjct: 138 TIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS----K 197

Query: 198 DIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDA 257
             ++ RD  GHGTHTAS  AG  V  ++ YGL  GTARGGVP+ARIAVYK+C ++GC   
Sbjct: 198 ANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGE 257

Query: 258 DILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYF 317
            +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP   
Sbjct: 258 AMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKIS 317

Query: 318 TIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVS 377
           T+ + +PW  SVAAS  +R  ++KV LG+  +  G ++NT+D+ G  YPL+Y  +A    
Sbjct: 318 TVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSA--AL 377

Query: 378 GGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSY 437
              +   +R C    +D  LV+GKI+LCDS               +V N    +   RS+
Sbjct: 378 STCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFIRSF 437

Query: 438 PLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILK 497
           P+  S+L      ++ +YM S K P AT+ KS  +++  APL+ SFSSRGP+    DILK
Sbjct: 438 PV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILK 497

Query: 498 PDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWS 557
           PD+TAPGVEILAA+SP +S +    D+R   Y+++SGTSM+CPH    A YVKTFHP WS
Sbjct: 498 PDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWS 557

Query: 558 PAAIKSALMTTAIPLNPKLN--PQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQ 617
           P+ I+SA+MTTA P+N   +     EFAYG+GH++P+ A+NPGLVY+  ++D++ FLCG 
Sbjct: 558 PSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGL 617

Query: 618 GYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNVGSKVA 677
            YT+  +R +SGD+S CT+  S  +  +LNYP+ +   + ++  N  F+RTVTNVG + +
Sbjct: 618 NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKS 677

Query: 678 TYRAKVLGAPRG-LTISVNPPALSFNAIGQKKSFTVTVRG---GVNQPIVSAALLWTDGH 737
           TY AKV+  P   L+I V+P  LS  ++ +K+SF VTV     G  QP VSA L+W+DG 
Sbjct: 678 TYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP-VSANLIWSDGT 737

Query: 738 HHVRSPITVYVV 740
           H+VRSPI VY +
Sbjct: 738 HNVRSPIIVYAM 740

BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 6.9e-184
Identity = 354/745 (47.52%), Postives = 477/745 (64.03%), Query Frame = 0

Query: 4   SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
           S IF L++ +SF+ +         D+  ++VYIVY+G  P      P SHH  +L++V G
Sbjct: 11  SCIFALLV-VSFASA-------GKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTG 70

Query: 64  STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
            +   D L+ +YKRSFNGF  +LTE E + +++ + VVSVFP+   +L TT SW+FMG  
Sbjct: 71  ESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLK 130

Query: 124 --KSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
             K  +R   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF CN K+I
Sbjct: 131 EGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLI 190

Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
           GAR Y       PE   S RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARI
Sbjct: 191 GARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARI 250

Query: 244 AVYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
           AVYK+C      C    ILAAFDDAIAD VDII++S+G +    +  D++AIGAFH+M  
Sbjct: 251 AVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAK 310

Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
           GILT N AGN+GP+  TI + +PW  +VAAS+++R  ++KV LGN     G ++N+FDL 
Sbjct: 311 GILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLN 370

Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
           GK+YPL+Y  +A   S     SS+ FCS   +D   V+GKI+LCD+  +P    +     
Sbjct: 371 GKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVA 430

Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
            +V N    +D +  +  P S L     N + +Y+ S K P A + KS  + +  AP++ 
Sbjct: 431 SIVRNP--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 490

Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
           S+SSRGPNP  +DILKPD+TAPG EILAA+SP    S   SD+R   Y +ISGTSMSCPH
Sbjct: 491 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPH 550

Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQ---AEFAYGAGHINPVKAVNPG 603
               A Y+KTFHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH++P+ A++PG
Sbjct: 551 VAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPG 610

Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW-DLNYPSFALSSTPSES 663
           LVY+A +SD++ FLCG  YT   +R +SGD S CT+  +  +  +LNYPS +   + ++ 
Sbjct: 611 LVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKP 670

Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
               FRRTVTNVG   ATY+AKV+G+   L + V P  LS  ++ +KKSFTVTV G    
Sbjct: 671 FKVTFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPK 730

Query: 724 NQPIVSAALLWTDGHHHVRSPITVY 738
            + +VSA L+W+DG H VRSPI VY
Sbjct: 731 AENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of CmaCh04G005370 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 4.2e-181
Identity = 356/748 (47.59%), Postives = 476/748 (63.64%), Query Frame = 0

Query: 4   SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
           S IF L++ +SF      +S D  D D ++ YIVY+G  P      P SHH  +L++V G
Sbjct: 12  SCIFALLV-VSF------ASADKDDQD-KQEYIVYMGALPARVDYMPMSHHTSILQDVTG 71

Query: 64  STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
            +   D L+ +YKRSFNGF  +LT+ E + +++ + VVSVFPN K  L TT SW+FMG  
Sbjct: 72  ESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLK 131

Query: 124 KSVRRVKQ--VESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
           +S R  +   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF  N K+I
Sbjct: 132 ESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLI 191

Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
           GAR Y       PE   S RD  GHG+HTAST AG  V   S YGL  GTARGGVP+ARI
Sbjct: 192 GARYYTPKLEGFPE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARI 251

Query: 244 AVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
           AVYK+C    DGC    ILAAFDDAIAD VDII++S+GG+    +  D IAIGAFH+M  
Sbjct: 252 AVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAK 311

Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
           GIL  NSAGN GP+  T+ + +PW  +VAAS+ +R  V+KV LGN     G ++N+FDL 
Sbjct: 312 GILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLN 371

Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
           GK+YPL+Y  +A +  G    +S+ FCS   +D   V+GKI+LCDS  +P    +     
Sbjct: 372 GKKYPLVYGKSASSSCG---AASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIA 431

Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
            +V +     D +  +  P S L     N + +YM S K P A + KS  + +  AP++ 
Sbjct: 432 SIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVA 491

Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
           S+ SRGPN    DILKPD+TAPG EI+AA+SP A  S  +SD+R   Y++ +GTSMSCPH
Sbjct: 492 SYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPH 551

Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNP---QAEFAYGAGHINPVKAVNPG 603
               A Y+K+FHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH++P+ A++PG
Sbjct: 552 VAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPG 611

Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSES 663
           LVY+A +SD++ FLCG  YT   +R +SGD S CT+  +  +  +LNYPS     + ++ 
Sbjct: 612 LVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKP 671

Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
               FRRTVTNVG   ATY+AKV+G+   L + V P  LS  ++ +KKSFTVT  G    
Sbjct: 672 FKVIFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTASGAGPK 731

Query: 724 NQPIVSAALLWTDGHHHVRSPITVYVVD 741
            + +VSA L+W+DG H VRSPI VY  +
Sbjct: 732 AENLVSAQLIWSDGVHFVRSPIVVYATN 738

BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match: A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 742/742 (100.00%), Postives = 742/742 (100.00%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
           QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
           QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match: A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)

HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 734/742 (98.92%), Postives = 737/742 (99.33%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKLVIFISFSGSLLAS LDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
           QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMNDAGVKDN+RSYPLPSSYLGPVAGNNIKTYMGS+KFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
           QESDYVRFLCGQGYTTAMVRRLSGDHSVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match: A0A5A7UBK2 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001150 PE=3 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 662/741 (89.34%), Postives = 703/741 (94.87%), Query Frame = 0

Query: 2   SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61
           SSSL+FK V+F S   SLLAS LD SDNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVV
Sbjct: 3   SSSLVFKFVVFSSLFCSLLASGLD-SDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVV 62

Query: 62  GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121
           GS+FAP+ALLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPN KKHLHTTRSWDFMGF
Sbjct: 63  GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 122

Query: 122 TKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181
           T++V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG
Sbjct: 123 TQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 182

Query: 182 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241
           ARAYRSD  FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIA
Sbjct: 183 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIA 242

Query: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301
           VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGIL
Sbjct: 243 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGIL 302

Query: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361
           TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQ
Sbjct: 303 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQ 362

Query: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421
           YPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S NGA+GVV
Sbjct: 363 YPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVV 422

Query: 422 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 481
           MND GVKDN+RSYPLPSSYL PV GNNIKTYM  NKFPTATI KSNAVNDTSAP IVSFS
Sbjct: 423 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 482

Query: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541
           SRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV DSRTTLYNIISGTSMSCPHATA
Sbjct: 483 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 542

Query: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601
           AAVYVKTFHP+WSPAAIKSALMTTA PLN KLN Q EFAYGAGHINP+KAV+PGL+YDA 
Sbjct: 543 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 602

Query: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 661
           E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRR
Sbjct: 603 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 662

Query: 662 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721
           TVTNVGSKV+TYRAKV+GAPRGL+I+VNPP LSFNAIGQKKSFT+T+RG + Q IVSA+L
Sbjct: 663 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 722

Query: 722 LWTDGHHHVRSPITVYVVDKA 743
           +W+DG+H+VRSPITV+VV  A
Sbjct: 723 MWSDGYHNVRSPITVFVVGTA 742

BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match: A0A1S3CFE1 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 662/741 (89.34%), Postives = 703/741 (94.87%), Query Frame = 0

Query: 2   SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61
           SSSL+FK V+F S   SLLAS LD SDNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVV
Sbjct: 15  SSSLVFKFVVFSSLFCSLLASGLD-SDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVV 74

Query: 62  GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121
           GS+FAP+ALLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPN KKHLHTTRSWDFMGF
Sbjct: 75  GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 134

Query: 122 TKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181
           T++V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG
Sbjct: 135 TQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 194

Query: 182 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241
           ARAYRSD  FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIA
Sbjct: 195 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIA 254

Query: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301
           VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGIL
Sbjct: 255 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGIL 314

Query: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361
           TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQ
Sbjct: 315 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQ 374

Query: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421
           YPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S NGA+GVV
Sbjct: 375 YPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVV 434

Query: 422 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 481
           MND GVKDN+RSYPLPSSYL PV GNNIKTYM  NKFPTATI KSNAVNDTSAP IVSFS
Sbjct: 435 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 494

Query: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541
           SRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV DSRTTLYNIISGTSMSCPHATA
Sbjct: 495 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 554

Query: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601
           AAVYVKTFHP+WSPAAIKSALMTTA PLN KLN Q EFAYGAGHINP+KAV+PGL+YDA 
Sbjct: 555 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 614

Query: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 661
           E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRR
Sbjct: 615 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 674

Query: 662 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721
           TVTNVGSKV+TYRAKV+GAPRGL+I+VNPP LSFNAIGQKKSFT+T+RG + Q IVSA+L
Sbjct: 675 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 734

Query: 722 LWTDGHHHVRSPITVYVVDKA 743
           +W+DG+H+VRSPITV+VV  A
Sbjct: 735 MWSDGYHNVRSPITVFVVGTA 754

BLAST of CmaCh04G005370 vs. ExPASy TrEMBL
Match: A0A6J1K719 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)

HSP 1 Score: 1295.8 bits (3352), Expect = 0.0e+00
Identity = 632/742 (85.18%), Postives = 697/742 (93.94%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           M SS+IFKLV F+ F G LLASSLD SDNDGRKVYIVYLGNKP+D ASTPSHHMR+LEEV
Sbjct: 13  MGSSVIFKLV-FLGFFGCLLASSLD-SDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEV 72

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTF+PDALLHSYKRSFNGF V+LTEEEAQKI AK+GVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 73  VGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMG 132

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FT +V RV QVES+IVVGVLDSGIWPESPSFSDVGYGP PAKWKGACQ S NFRCN+KII
Sbjct: 133 FTTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKII 192

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSDN FPPED +SPRDSDGHGTHTASTVAGGLV+QASLYGLALGTARGGVPSARI
Sbjct: 193 GARAYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARI 252

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG++PKYYFNDSIAIGAFHSMKHGI
Sbjct: 253 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGI 312

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS VQLGNKN++QGYTINTFDL+GK
Sbjct: 313 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGK 372

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
            YPLIYAG+APN+SGGF+GSSSR+CS+NSVDRNLV+GKIL+CDSILSPSTFAS + AVGV
Sbjct: 373 HYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGV 432

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMN+AGVKDN+RSYPLPSSYL   AGN++K Y+GSN FPTATIFKSN VNDT+AP++VSF
Sbjct: 433 VMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSF 492

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAW+PIA VSSG+ DSRTTLYNIISGTSMSCPHAT
Sbjct: 493 SSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHAT 552

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNPQAEFAYGAGHI+P+KA+NPGL+YDA
Sbjct: 553 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDA 612

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
            E+DYV FLCGQGY+TAMVRRL+GD SVCT ANSGRVWDLNYPSFALS+TPSESINQFF 
Sbjct: 613 NETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFT 672

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RT+TNVGS+ +TY +K+LGAP GLTI+V+PP LSFN IG K+SFT+T+ G ++Q IVSA+
Sbjct: 673 RTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSAS 732

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           ++W+DG H+VRSPIT+Y+V+KA
Sbjct: 733 VVWSDGSHNVRSPITIYIVNKA 752

BLAST of CmaCh04G005370 vs. NCBI nr
Match: XP_022979186.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 742/742 (100.00%), Postives = 742/742 (100.00%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
           QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
           QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of CmaCh04G005370 vs. NCBI nr
Match: XP_022941960.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 734/742 (98.92%), Postives = 737/742 (99.33%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKLVIFISFSGSLLAS LDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
           QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMNDAGVKDN+RSYPLPSSYLGPVAGNNIKTYMGS+KFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
           QESDYVRFLCGQGYTTAMVRRLSGDHSVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of CmaCh04G005370 vs. NCBI nr
Match: KAG6600369.1 (hypothetical protein SDJN03_05602, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1471.1 bits (3807), Expect = 0.0e+00
Identity = 733/742 (98.79%), Postives = 736/742 (99.19%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKLVIFISFSG LLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGCLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
           QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMNDAGVKDN+R+YPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARTYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAWSPI SVSSGVSDSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIVSVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
           QESDYVRFLCGQGYTTAMVRRLSGDHSVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of CmaCh04G005370 vs. NCBI nr
Match: XP_023536304.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 731/742 (98.52%), Postives = 735/742 (99.06%), Query Frame = 0

Query: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60
           MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120
           VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180
           FTK+VRRVKQVESNIVVGVLDSGIWPESPSFSDVGYG PPAKWKGACQTSANFRCNRKI+
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIV 180

Query: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240
           GARAYRSD  FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360
           LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420
           QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGV 420

Query: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480
           VMNDAGVKDN+RSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSN VNDTSAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSF 480

Query: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV DSRTTLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHAT 540

Query: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600
           AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660
           QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720
           RTVTNVGSKVATYRAKVLG PRGLTISVNPP LSFNAIGQKKSFTVTVRGGVNQPIVSAA
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 721 LLWTDGHHHVRSPITVYVVDKA 743
           LLWTDGHHHVRSPITVYVVDKA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of CmaCh04G005370 vs. NCBI nr
Match: XP_008461729.2 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 662/741 (89.34%), Postives = 703/741 (94.87%), Query Frame = 0

Query: 2   SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61
           SSSL+FK V+F S   SLLAS LD SDNDGRK+YIVY+GNKPEDSASTPSHHMRMLEEVV
Sbjct: 15  SSSLVFKFVVFSSLFCSLLASGLD-SDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVV 74

Query: 62  GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121
           GS+FAP+ALLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPN KKHLHTTRSWDFMGF
Sbjct: 75  GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 134

Query: 122 TKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181
           T++V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG
Sbjct: 135 TQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 194

Query: 182 ARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241
           ARAYRSD  FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIA
Sbjct: 195 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIA 254

Query: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301
           VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN+PKYYFNDSIAIGAFHSMKHGIL
Sbjct: 255 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGIL 314

Query: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361
           TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVS+VQLGNKN+YQGYTINTFDLKGKQ
Sbjct: 315 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQ 374

Query: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421
           YPLIYAG+APN+SGGFTGSSSRFCSRNSVDRNLV+GKI+LCDS+LSP+TF S NGA+GVV
Sbjct: 375 YPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVV 434

Query: 422 MNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFS 481
           MND GVKDN+RSYPLPSSYL PV GNNIKTYM  NKFPTATI KSNAVNDTSAP IVSFS
Sbjct: 435 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 494

Query: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541
           SRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGV DSRTTLYNIISGTSMSCPHATA
Sbjct: 495 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 554

Query: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601
           AAVYVKTFHP+WSPAAIKSALMTTA PLN KLN Q EFAYGAGHINP+KAV+PGL+YDA 
Sbjct: 555 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 614

Query: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRR 661
           E DYVRFLCGQGYTTAMVRRLSGD+S C+RANSGRVWDLNYPSFALSST ++S NQFFRR
Sbjct: 615 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 674

Query: 662 TVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721
           TVTNVGSKV+TYRAKV+GAPRGL+I+VNPP LSFNAIGQKKSFT+T+RG + Q IVSA+L
Sbjct: 675 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 734

Query: 722 LWTDGHHHVRSPITVYVVDKA 743
           +W+DG+H+VRSPITV+VV  A
Sbjct: 735 MWSDGYHNVRSPITVFVVGTA 754

BLAST of CmaCh04G005370 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 665.6 bits (1716), Expect = 4.6e-191
Identity = 343/705 (48.65%), Postives = 467/705 (66.24%), Query Frame = 0

Query: 39  LGNKPEDSASTPSHHMRMLEEVVGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEG 98
           +G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF   L++ E+QK+   + 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 99  VVSVFPNGKKHLHTTRSWDFMGFTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 158
           VVSVFP+    L TTRSWDF+GF +  RR    ES+++VGV+DSGIWPES SF D G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 159 PPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLV 218
           PP KWKG+C+    F CN K+IGAR Y   N F      S RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY---NKF----ADSARDEEGHGTHTASTAAGNAV 180

Query: 219 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 278
             AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+  +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 279 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSK 338
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 339 VQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGK 398
           V LGN     G ++NTF+L G ++P++Y     NVS   + + + +CS   VD  LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 399 ILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKF 458
           I+LCD  L     A   GA+GV++ +  + D++   P P+S LG     +IK+Y+ S + 
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 459 PTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV 518
           P A I ++  + D  AP + SFSSRGP+    ++LKPD++APG+EILAA+SP+AS SS +
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 519 S--DSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQ 578
           +  D R+  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTA P+N K NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 579 AEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGR 638
            EFAYG+G INP KA +PGLVY+ +  DY++ LC +G+ +  +   SG +  C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 639 VWDLNYPSFALSSTPSESINQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFN 698
           V DLNYP+     +  +  N  F+RTVTNVG   +TY+A V+     L IS+ P  L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 699 AIGQKKSFTVTVRGG--VNQPIVSAALLWTDGHHHVRSPITVYVV 740
            + +KKSF VT+ G    +   VS++++W+DG H VRSPI  Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of CmaCh04G005370 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 646.4 bits (1666), Expect = 2.9e-185
Identity = 342/732 (46.72%), Postives = 479/732 (65.44%), Query Frame = 0

Query: 18  SLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVGSTFAPDALLHSYKR 77
           SL + S D  D+  ++VYIVYLG+ P     TP S HM +L+E+ G +   + L+ SYK+
Sbjct: 18  SLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKK 77

Query: 78  SFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFTKSV--RRVKQVESN 137
           SFNGF  +LTE E ++++  E VVSVFP+ K  L TT SW+FMG  + +  +R + +ES+
Sbjct: 78  SFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESD 137

Query: 138 IVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGARAYRSDNLFPPE 197
            ++GV+DSGI+PES SFSD G+GPPP KWKG C    NF CN K+IGAR Y + +    +
Sbjct: 138 TIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS----K 197

Query: 198 DIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDA 257
             ++ RD  GHGTHTAS  AG  V  ++ YGL  GTARGGVP+ARIAVYK+C ++GC   
Sbjct: 198 ANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGE 257

Query: 258 DILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYF 317
            +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP   
Sbjct: 258 AMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKIS 317

Query: 318 TIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQYPLIYAGNAPNVS 377
           T+ + +PW  SVAAS  +R  ++KV LG+  +  G ++NT+D+ G  YPL+Y  +A    
Sbjct: 318 TVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSA--AL 377

Query: 378 GGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVMNDAGVKDNSRSY 437
              +   +R C    +D  LV+GKI+LCDS               +V N    +   RS+
Sbjct: 378 STCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFIRSF 437

Query: 438 PLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSSRGPNPETYDILK 497
           P+  S+L      ++ +YM S K P AT+ KS  +++  APL+ SFSSRGP+    DILK
Sbjct: 438 PV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILK 497

Query: 498 PDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPSWS 557
           PD+TAPGVEILAA+SP +S +    D+R   Y+++SGTSM+CPH    A YVKTFHP WS
Sbjct: 498 PDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWS 557

Query: 558 PAAIKSALMTTAIPLNPKLN--PQAEFAYGAGHINPVKAVNPGLVYDAQESDYVRFLCGQ 617
           P+ I+SA+MTTA P+N   +     EFAYG+GH++P+ A+NPGLVY+  ++D++ FLCG 
Sbjct: 558 PSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGL 617

Query: 618 GYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRRTVTNVGSKVA 677
            YT+  +R +SGD+S CT+  S  +  +LNYP+ +   + ++  N  F+RTVTNVG + +
Sbjct: 618 NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKS 677

Query: 678 TYRAKVLGAPRG-LTISVNPPALSFNAIGQKKSFTVTVRG---GVNQPIVSAALLWTDGH 737
           TY AKV+  P   L+I V+P  LS  ++ +K+SF VTV     G  QP VSA L+W+DG 
Sbjct: 678 TYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP-VSANLIWSDGT 737

Query: 738 HHVRSPITVYVV 740
           H+VRSPI VY +
Sbjct: 738 HNVRSPIIVYAM 740

BLAST of CmaCh04G005370 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 645.6 bits (1664), Expect = 4.9e-185
Identity = 354/745 (47.52%), Postives = 477/745 (64.03%), Query Frame = 0

Query: 4   SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
           S IF L++ +SF+ +         D+  ++VYIVY+G  P      P SHH  +L++V G
Sbjct: 11  SCIFALLV-VSFASA-------GKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTG 70

Query: 64  STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
            +   D L+ +YKRSFNGF  +LTE E + +++ + VVSVFP+   +L TT SW+FMG  
Sbjct: 71  ESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLK 130

Query: 124 --KSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
             K  +R   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF CN K+I
Sbjct: 131 EGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLI 190

Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
           GAR Y       PE   S RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARI
Sbjct: 191 GARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARI 250

Query: 244 AVYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
           AVYK+C      C    ILAAFDDAIAD VDII++S+G +    +  D++AIGAFH+M  
Sbjct: 251 AVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAK 310

Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
           GILT N AGN+GP+  TI + +PW  +VAAS+++R  ++KV LGN     G ++N+FDL 
Sbjct: 311 GILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLN 370

Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
           GK+YPL+Y  +A   S     SS+ FCS   +D   V+GKI+LCD+  +P    +     
Sbjct: 371 GKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVA 430

Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
            +V N    +D +  +  P S L     N + +Y+ S K P A + KS  + +  AP++ 
Sbjct: 431 SIVRNP--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 490

Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
           S+SSRGPNP  +DILKPD+TAPG EILAA+SP    S   SD+R   Y +ISGTSMSCPH
Sbjct: 491 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPH 550

Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQ---AEFAYGAGHINPVKAVNPG 603
               A Y+KTFHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH++P+ A++PG
Sbjct: 551 VAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPG 610

Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVW-DLNYPSFALSSTPSES 663
           LVY+A +SD++ FLCG  YT   +R +SGD S CT+  +  +  +LNYPS +   + ++ 
Sbjct: 611 LVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKP 670

Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
               FRRTVTNVG   ATY+AKV+G+   L + V P  LS  ++ +KKSFTVTV G    
Sbjct: 671 FKVTFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPK 730

Query: 724 NQPIVSAALLWTDGHHHVRSPITVY 738
            + +VSA L+W+DG H VRSPI VY
Sbjct: 731 AENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of CmaCh04G005370 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 636.3 bits (1640), Expect = 3.0e-182
Identity = 356/748 (47.59%), Postives = 476/748 (63.64%), Query Frame = 0

Query: 4   SLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEVVG 63
           S IF L++ +SF      +S D  D D ++ YIVY+G  P      P SHH  +L++V G
Sbjct: 12  SCIFALLV-VSF------ASADKDDQD-KQEYIVYMGALPARVDYMPMSHHTSILQDVTG 71

Query: 64  STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 123
            +   D L+ +YKRSFNGF  +LT+ E + +++ + VVSVFPN K  L TT SW+FMG  
Sbjct: 72  ESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLK 131

Query: 124 KSVRRVKQ--VESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 183
           +S R  +   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF  N K+I
Sbjct: 132 ESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLI 191

Query: 184 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 243
           GAR Y       PE   S RD  GHG+HTAST AG  V   S YGL  GTARGGVP+ARI
Sbjct: 192 GARYYTPKLEGFPE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARI 251

Query: 244 AVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 303
           AVYK+C    DGC    ILAAFDDAIAD VDII++S+GG+    +  D IAIGAFH+M  
Sbjct: 252 AVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAK 311

Query: 304 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 363
           GIL  NSAGN GP+  T+ + +PW  +VAAS+ +R  V+KV LGN     G ++N+FDL 
Sbjct: 312 GILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLN 371

Query: 364 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 423
           GK+YPL+Y  +A +  G    +S+ FCS   +D   V+GKI+LCDS  +P    +     
Sbjct: 372 GKKYPLVYGKSASSSCG---AASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIA 431

Query: 424 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 483
            +V +     D +  +  P S L     N + +YM S K P A + KS  + +  AP++ 
Sbjct: 432 SIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVA 491

Query: 484 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 543
           S+ SRGPN    DILKPD+TAPG EI+AA+SP A  S  +SD+R   Y++ +GTSMSCPH
Sbjct: 492 SYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPH 551

Query: 544 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNP---QAEFAYGAGHINPVKAVNPG 603
               A Y+K+FHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH++P+ A++PG
Sbjct: 552 VAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPG 611

Query: 604 LVYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRV-WDLNYPSFALSSTPSES 663
           LVY+A +SD++ FLCG  YT   +R +SGD S CT+  +  +  +LNYPS     + ++ 
Sbjct: 612 LVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKP 671

Query: 664 INQFFRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGG--V 723
               FRRTVTNVG   ATY+AKV+G+   L + V P  LS  ++ +KKSFTVT  G    
Sbjct: 672 FKVIFRRTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTASGAGPK 731

Query: 724 NQPIVSAALLWTDGHHHVRSPITVYVVD 741
            + +VSA L+W+DG H VRSPI VY  +
Sbjct: 732 AENLVSAQLIWSDGVHFVRSPIVVYATN 738

BLAST of CmaCh04G005370 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 629.4 bits (1622), Expect = 3.7e-180
Identity = 348/748 (46.52%), Postives = 470/748 (62.83%), Query Frame = 0

Query: 2   SSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTP-SHHMRMLEEV 61
           SSSL+  L++       L  SS+ S+  D ++VYIVY+G+    +  TP S HM +L+EV
Sbjct: 7   SSSLLSCLLV-------LFLSSV-SAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEV 66

Query: 62  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 121
            G +     L+ SYKRSFNGF  +LTE E ++++   GVVSVFPN K  L TT SWDFMG
Sbjct: 67  TGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 126

Query: 122 FTKSV--RRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRK 181
             + +  +R   VES+ ++GV+DSGI PES SFSD G+GPPP KWKG C    NF CN K
Sbjct: 127 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNK 186

Query: 182 IIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSA 241
           +IGAR Y S+           RD DGHGTHTAST AG  V  AS +G+  GT RGGVP++
Sbjct: 187 LIGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 246

Query: 242 RIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 301
           R+A YK+C   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  
Sbjct: 247 RVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 306

Query: 302 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 361
           G+LT NSAGN GP   ++   +PW L+VAAS+ +R  V+KV LGN     G ++N +++K
Sbjct: 307 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 366

Query: 362 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 421
           GK YPL+Y  +A   S      S+  C  + VD++ V+GKIL+C          S  GAV
Sbjct: 367 GKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAV 426

Query: 422 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 481
           G++       D +  +PLP++ L      ++ +Y+ S   P A + K+ A+ + ++P+I 
Sbjct: 427 GLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIA 486

Query: 482 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 541
           SFSSRGPN    DILKPD+TAPGVEILAA+SP    S    D+R   Y+++SGTSMSCPH
Sbjct: 487 SFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPH 546

Query: 542 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLN--PQAEFAYGAGHINPVKAVNPGL 601
               A YVKTF+P WSP+ I+SA+MTTA P+N         EFAYG+GH++P+ A NPGL
Sbjct: 547 VAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGL 606

Query: 602 VYDAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSF-ALSSTPSESI 661
           VY+  +SD++ FLCG  YT+ +++ +SG+   C+ A      +LNYPS  A  S    + 
Sbjct: 607 VYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTF 666

Query: 662 NQFFRRTVTNVGSKVATYRAKVL-GAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQ 721
              F RT+TNVG+  +TY +KV+ G    L + + P  LSF  + +K+SFTVTV G    
Sbjct: 667 TVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLD 726

Query: 722 PIV--SAALLWTDGHHHVRSPITVYVVD 741
             V  SA L+W+DG H+VRSPI VY  D
Sbjct: 727 SEVPSSANLIWSDGTHNVRSPIVVYTSD 731

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q395472.8e-27063.36Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF87.4e-19448.89Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9FGU34.1e-18446.72Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... [more]
Q9STF76.9e-18447.52Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... [more]
F4JA914.2e-18147.59Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A6J1IQ270.0e+00100.00cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1[more]
A0A6J1FV970.0e+0098.92cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1[more]
A0A5A7UBK20.0e+0089.34Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00115... [more]
A0A1S3CFE10.0e+0089.34cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1[more]
A0A6J1K7190.0e+0085.18cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022979186.10.0e+00100.00cucumisin-like [Cucurbita maxima][more]
XP_022941960.10.0e+0098.92cucumisin-like [Cucurbita moschata][more]
KAG6600369.10.0e+0098.79hypothetical protein SDJN03_05602, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023536304.10.0e+0098.52cucumisin-like [Cucurbita pepo subsp. pepo][more]
XP_008461729.20.0e+0089.34PREDICTED: cucumisin-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G59190.14.6e-19148.65subtilase family protein [more]
AT5G59100.12.9e-18546.72Subtilisin-like serine endopeptidase family protein [more]
AT3G46850.14.9e-18547.52Subtilase family protein [more]
AT3G46840.13.0e-18247.59Subtilase family protein [more]
AT5G59120.13.7e-18046.52subtilase 4.13 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 132..151
score: 32.79
coord: 201..214
score: 52.19
coord: 530..546
score: 43.08
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 133..584
e-value: 3.3E-47
score: 161.1
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 34..110
e-value: 9.1E-15
score: 55.1
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 639..736
e-value: 1.7E-26
score: 92.3
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 134..605
e-value: 3.6E-186
score: 621.5
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 113..593
NoneNo IPR availableGENE3D3.50.30.30coord: 332..470
e-value: 3.6E-186
score: 621.5
NoneNo IPR availableGENE3D2.60.40.2310coord: 609..739
e-value: 1.4E-40
score: 140.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 192..211
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 23..737
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 115..592
score: 26.022621
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 339..463
e-value: 2.16979E-16
score: 73.9871
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 18..111
e-value: 1.6E-27
score: 97.8
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 23..737
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 531..541
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 108..567
e-value: 1.07061E-127
score: 380.405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G005370.1CmaCh04G005370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity