CmaCh04G003620 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G003620
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionspeckle-type POZ protein-like A
LocationCma_Chr04: 1809170 .. 1811410 (+)
RNA-Seq ExpressionCmaCh04G003620
SyntenyCmaCh04G003620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATTCACACATAACCATTATGGTTGACAATATGTCGTACGATGATCCTATCAAACATGAACAACAACCTGGTACGTATCCCAAATACCTACAATTTTTCTTTTTCAAATATCTTTACCATTAGTTTTGGATGGAACCGTGTATCTACGCTTTATAGATCATGTTTAACTTTAATTAATTTAACGTACTAATGTTTTTGTTAATACAGAAATTACAAGAACAACAAGGGTTGACCCACCAGCTAATTACATATTCCAAATTAATTCATACTCAACACTTTCAGAAATAGGGATGAAGAAATGTGAATCTGGACACTTTGCAGTTGATGGCTACAACTGGTTTGAAATGCTTAGATTTCAATTTAAACTTGATTAATATCATAAACTATGATTAGGGTTTTAATTAATCTCTCTCTATCTTACATGGGTTGGCTTAGGAAATTGATTTTGTTTCCAAATGGAGACGACAAAGTTGAAGATCATATCTCTCTCTACTTGGCTATAACTTCTAAGGATAATCTTCCTCTTGGCTGGGAAGTGAAGGTTATTTTCAGGTTCTTTGTATACGATCAAATCAAAGACAACTATTTAACCATCCAAGGTATCGATATATCTGTTGTTAGGAATCTTCATAATGGTATGATATTGTCCATTTTGAGCATAAACTCTCGTGGTTTAACCGATGATCATTCCCTAAATTAGTCAATGTGGGACTCCCCCCAACATTCCTCCCCTCGAACAAAGTACACTATAGAGCCTCCTCTGAGGCCTATGGAGCACTTGAACAACCTCCCCTTGATTGAGGCTCGACTCCTTCTCTGGAATTCTCGAACAAAGTACACCATTTGTTCCACACTTGAGTCTCATAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATAGCTACGTTAGGACACAGTCATGCGTTTATAAGTAAGTAATAAATTTCGTATGAGACCTTTTAGGAAAGCCCAAAGAAAAACGATAAAAGCTTATGCTCGAAGTGGATAATATCAAACTATCTTAGAGAGTCGTGATTCCTATATTTCATTCGATTTTTATTCATTAAACTAATCGAATAGTTATCGAACATGACTTTAGATAACATGATCGAGACTTAAAAACGAATATCGATAATGATTTTGCAGATGGGAAAATGAGGAAGTATTCTAAAATGAAGACTGAACATGGGTTCACCAAATTTCTTGCTCATAATGCTTTCAATCAAGCCTCCAATGGCTTCCTTGTGGATAATGGCTGCACATTTGGAGTTGAAGTTTCTGTACTTAAAGCCTCAAACAAAGGAGAGAGTTTAACCATTTTTAAAGAGCCTCAACTTGGATCCTTTTTTTGGTCCATTTCTTCATTTTCTAACAAAAATAATGAATTCTATTTCTCCGAACCCTTCACTGTGAAAGGAAGAAAATGGTACGTAACGTGAAAAGAAAAACAAAATTGTTATCAAACGAGATTTACGAAAGACTCATTTTTCGTTTGACTTATTATTTCAGGCGATTGATGATATACCCGAACGGTTTTTTGTCGGGAAAGACGTCACATATCTCTCTTTACTTGAAGTTGGATAGCTCGGAAACCATTCCGACCGGGAAGAAAATCTATGCCAAATTCTTTCTAAGTGTTTATAACTTTGGAGCTAAAAAATATGTGGATCAATCAAGTAAGTTATTTTTCTTTGAGTTTTTTTAATGAAAATAAAGTATTTTGTGTTGGAATCTTATGTGAAGCATTGCATTATTTGCAGATGAGTATTGGTTTGATAACACAGCCTATGGTCATGGCTTTCATGAGTTCTTGTCTCGAAACGACCTTCAACCTCATCATTTGCAAAATGATGTTTTCTATCTCAAAGCTAGAATTGTTGCTATGTCGGTCGTCCAAGATTTCTAGGTCAAAATGACATCAACAACCCGTGTCGAGATGTTGTATTAATATATGATAATGTATATATAGTACATAACGTGATACATGTGCTCGTGTTTAAGAGTTGGTCAATGATGAATTCTCGACCAAAAAAAATATTTATCTTGAAAAAGTATGTAAATCTCGTTAGTTCGAGTATAACTCAGATAAGAAATAATGATTTACAAACGAAAAGATCGAGTCAAGTAGATAGAACCTACTCGAAAAATCGTCTTATGTAAGAGAAAAAGCCCACCATTAACCGATATTGTCCTCTTTGGGCCTTTCTTCAAGGTTTTAAAATGCGTCTACTACGAAG

mRNA sequence

ACATTCACACATAACCATTATGGTTGACAATATGTCGTACGATGATCCTATCAAACATGAACAACAACCTGAAATTACAAGAACAACAAGGGTTGACCCACCAGCTAATTACATATTCCAAATTAATTCATACTCAACACTTTCAGAAATAGGGATGAAGAAATGTGAATCTGGACACTTTGCAGTTGATGGCTACAACTGGAAATTGATTTTGTTTCCAAATGGAGACGACAAAGTTGAAGATCATATCTCTCTCTACTTGGCTATAACTTCTAAGGATAATCTTCCTCTTGGCTGGGAAGTGAAGGTTATTTTCAGGTTCTTTGTATACGATCAAATCAAAGACAACTATTTAACCATCCAAGATGGGAAAATGAGGAAGTATTCTAAAATGAAGACTGAACATGGGTTCACCAAATTTCTTGCTCATAATGCTTTCAATCAAGCCTCCAATGGCTTCCTTGTGGATAATGGCTGCACATTTGGAGTTGAAGTTTCTGTACTTAAAGCCTCAAACAAAGGAGAGAGTTTAACCATTTTTAAAGAGCCTCAACTTGGATCCTTTTTTTGGTCCATTTCTTCATTTTCTAACAAAAATAATGAATTCTATTTCTCCGAACCCTTCACTGTGAAAGGAAGAAAATGGCGATTGATGATATACCCGAACGGTTTTTTGTCGGGAAAGACGTCACATATCTCTCTTTACTTGAAGTTGGATAGCTCGGAAACCATTCCGACCGGGAAGAAAATCTATGCCAAATTCTTTCTAAGTGTTTATAACTTTGGAGCTAAAAAATATGTGGATCAATCAAATGAGTATTGGTTTGATAACACAGCCTATGGTCATGGCTTTCATGAGTTCTTGTCTCGAAACGACCTTCAACCTCATCATTTGCAAAATGATGTTTTCTATCTCAAAGCTAGAATTGTTGCTATGTCGGTCGTCCAAGATTTCTAGGTCAAAATGACATCAACAACCCGTGTCGAGATGTTGTATTAATATATGATAATGTATATATAGTACATAACGTGATACATGTGCTCGTGTTTAAGAGTTGGTCAATGATGAATTCTCGACCAAAAAAAATATTTATCTTGAAAAAGTATGTAAATCTCGTTAGTTCGAGTATAACTCAGATAAGAAATAATGATTTACAAACGAAAAGATCGAGTCAAGTAGATAGAACCTACTCGAAAAATCGTCTTATGTAAGAGAAAAAGCCCACCATTAACCGATATTGTCCTCTTTGGGCCTTTCTTCAAGGTTTTAAAATGCGTCTACTACGAAG

Coding sequence (CDS)

ATGGTTGACAATATGTCGTACGATGATCCTATCAAACATGAACAACAACCTGAAATTACAAGAACAACAAGGGTTGACCCACCAGCTAATTACATATTCCAAATTAATTCATACTCAACACTTTCAGAAATAGGGATGAAGAAATGTGAATCTGGACACTTTGCAGTTGATGGCTACAACTGGAAATTGATTTTGTTTCCAAATGGAGACGACAAAGTTGAAGATCATATCTCTCTCTACTTGGCTATAACTTCTAAGGATAATCTTCCTCTTGGCTGGGAAGTGAAGGTTATTTTCAGGTTCTTTGTATACGATCAAATCAAAGACAACTATTTAACCATCCAAGATGGGAAAATGAGGAAGTATTCTAAAATGAAGACTGAACATGGGTTCACCAAATTTCTTGCTCATAATGCTTTCAATCAAGCCTCCAATGGCTTCCTTGTGGATAATGGCTGCACATTTGGAGTTGAAGTTTCTGTACTTAAAGCCTCAAACAAAGGAGAGAGTTTAACCATTTTTAAAGAGCCTCAACTTGGATCCTTTTTTTGGTCCATTTCTTCATTTTCTAACAAAAATAATGAATTCTATTTCTCCGAACCCTTCACTGTGAAAGGAAGAAAATGGCGATTGATGATATACCCGAACGGTTTTTTGTCGGGAAAGACGTCACATATCTCTCTTTACTTGAAGTTGGATAGCTCGGAAACCATTCCGACCGGGAAGAAAATCTATGCCAAATTCTTTCTAAGTGTTTATAACTTTGGAGCTAAAAAATATGTGGATCAATCAAATGAGTATTGGTTTGATAACACAGCCTATGGTCATGGCTTTCATGAGTTCTTGTCTCGAAACGACCTTCAACCTCATCATTTGCAAAATGATGTTTTCTATCTCAAAGCTAGAATTGTTGCTATGTCGGTCGTCCAAGATTTCTAG

Protein sequence

MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLGSFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLKARIVAMSVVQDF
Homology
BLAST of CmaCh04G003620 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.2e-12
Identity = 41/139 (29.50%), Postives = 76/139 (54.68%), Query Frame = 0

Query: 26  DPPA-NYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGDDKVEDHISLYLAIT 85
           DPP+  + + I  ++ L+    +K  S  F V GY W++++FP G++   DH+S+YL + 
Sbjct: 50  DPPSLKFTWTIPMFTRLN---TRKHYSDVFVVGGYKWRILIFPKGNN--VDHLSMYLDVA 109

Query: 86  SKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFLAHNAFNQAS 145
              NLP GW     F   V +Q+ + Y +I+     +++  +++ GFT F+  +   + +
Sbjct: 110 DAANLPYGWSRYSQFSLAVVNQVNNRY-SIRKETQHQFNARESDWGFTSFMPLSELYEPT 169

Query: 146 NGFLVDNGCTFGVEVSVLK 164
            G+LV++      EV+V K
Sbjct: 170 RGYLVNDTVLIEAEVAVRK 182

BLAST of CmaCh04G003620 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-12
Identity = 46/154 (29.87%), Postives = 79/154 (51.30%), Query Frame = 0

Query: 16  QPEITRTT-----RVDPPA-NYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNG 75
           QPE   +T       DPP   + + I ++S  +    +K  S  F V GY W++++FP G
Sbjct: 36  QPETAASTVENQPAEDPPTLKFTWTIPNFSRQN---TRKHYSDVFVVGGYKWRILIFPKG 95

Query: 76  DDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEH 135
           ++   DH+S+YL ++   +LP GW     F   V +QI   Y T++     +++  +++ 
Sbjct: 96  NN--VDHLSMYLDVSDAASLPYGWSRYAQFSLAVVNQIHTRY-TVRKETQHQFNARESDW 155

Query: 136 GFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLK 164
           GFT F+  +     S G+LV++      EV+V K
Sbjct: 156 GFTSFMPLSELYDPSRGYLVNDTVLVEAEVAVRK 183

BLAST of CmaCh04G003620 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-11
Identity = 40/121 (33.06%), Postives = 60/121 (49.59%), Query Frame = 0

Query: 47  KKCESGHFAVDGYNWKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQ 106
           +K  S  F VDG  W+L+ FP G+    + +SLYLA+   + LP GW     F F V +Q
Sbjct: 22  RKNYSDEFFVDGCKWRLLAFPKGNG--VEKLSLYLAVAGSEFLPDGWRRHAYFHFSVVNQ 81

Query: 107 IKDNYLTIQDGKMRKYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASN 166
           + D     ++ K   +    ++ GFT  L+    +    GFLV+      V+VSVL+   
Sbjct: 82  LSDELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKDGGFLVNGELKIVVDVSVLEVIG 139

Query: 167 K 168
           K
Sbjct: 142 K 139

BLAST of CmaCh04G003620 vs. ExPASy Swiss-Prot
Match: Q9SHT3 (MATH domain and coiled-coil domain-containing protein At2g05410 OS=Arabidopsis thaliana OX=3702 GN=At2g05410 PE=4 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-09
Identity = 41/129 (31.78%), Postives = 67/129 (51.94%), Query Frame = 0

Query: 184 WSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKK 243
           W I +FS+  +   +S+ F V G KWR+M YP G      S  SL+L +  +E++PTG +
Sbjct: 11  WVIKNFSSLQSAPIYSDIFVVGGCKWRIMAYPKGINKAHDS-FSLFLNVPDNESLPTGWR 70

Query: 244 IYAKFFLSVYNFGAKKYVDQS-NEYWFDNTAYGHGFHEFLSRNDLQPH--HLQNDVFYLK 303
            +AK   S+ N G++K   +   ++WF   A+  GF   ++  +L      L N    + 
Sbjct: 71  RHAKVSFSLVNQGSEKLSQRKVTQHWFVQKAFTWGFPVMITHTELNAKMGFLVNGELKVV 130

Query: 304 ARIVAMSVV 310
           A+I  + VV
Sbjct: 131 AKIEVLEVV 138

BLAST of CmaCh04G003620 vs. ExPASy Swiss-Prot
Match: Q9M2H9 (MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis thaliana OX=3702 GN=At3g58370 PE=4 SV=3)

HSP 1 Score: 62.8 bits (151), Expect = 8.1e-09
Identity = 37/131 (28.24%), Postives = 64/131 (48.85%), Query Frame = 0

Query: 182 FFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTG 241
           F W I +FS    +  +S+PF + G KW L+ YP G  +   + +SLYL +D +  +P G
Sbjct: 10  FTWVIKNFSTLQYDKIYSDPFVIGGCKWHLLAYPKG--NKFNNSLSLYLVVDDARALPCG 69

Query: 242 KKIYAKFFLSVYNFGAKKYVDQ-SNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 301
            + YA+F L++ N    K   +   + WF+    G GF   +   +L   H +N  + + 
Sbjct: 70  WRRYAQFSLTIINQLTDKLSQRGEKQNWFNQRNLGSGFTSMIPLPNL---HAKNAGYLVN 129

Query: 302 ARIVAMSVVQD 312
             +  +  + D
Sbjct: 130 GEVKIVVEIND 135

BLAST of CmaCh04G003620 vs. ExPASy TrEMBL
Match: A0A6J1JFK1 (uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.5e-183
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN
Sbjct: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR
Sbjct: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
           KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT
Sbjct: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK
Sbjct: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300

Query: 301 ARIVAMSVVQDF 313
           ARIVAMSVVQDF
Sbjct: 301 ARIVAMSVVQDF 312

BLAST of CmaCh04G003620 vs. ExPASy TrEMBL
Match: A0A6J1FUL4 (speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE=4 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 3.6e-169
Identity = 286/312 (91.67%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           MVDNMSYDDPIKHEQQPEITRT R +PPANY FQINSYSTLSEIGMKKCESG+FAVDG+N
Sbjct: 1   MVDNMSYDDPIKHEQQPEITRTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFN 60

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLIL+PNGDD+VEDHISLYLAITSKDNLPLGWEVKVIFRFFVYD+IKDNYLT+QDGKMR
Sbjct: 61  WKLILYPNGDDEVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMR 120

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
           KYSKMK+EHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 121 KYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNK NEFY SEPFTVKGRKWRLMIYPNG  SGK SHISLYLKLDSSET+PT
Sbjct: 181 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSETVPT 240

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVY+FGAK+YVDQSNE+WFDNT YG+GFHEFLSRNDL+PHHLQND FYLK
Sbjct: 241 GKKIYAKFFLSVYDFGAKRYVDQSNEHWFDNTTYGYGFHEFLSRNDLRPHHLQNDGFYLK 300

Query: 301 ARIVAMSVVQDF 313
           ARI AMSVV+DF
Sbjct: 301 ARIAAMSVVEDF 312

BLAST of CmaCh04G003620 vs. ExPASy TrEMBL
Match: A0A5A7TNH7 (Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00430 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 9.9e-127
Identity = 224/315 (71.11%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEIT-RTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGY 60
           M DNMSYD+ IK E+Q EIT ++ RVDPP++Y FQI+SYS LSEI MKKCESG F VDGY
Sbjct: 1   MDDNMSYDESIKDERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGY 60

Query: 61  NWKLILFPNGDDKVEDHISLYLAITSKD--NLPLGWEVKVIFRFFVYDQIKDNYLTIQDG 120
            WKLIL+PNG+ +VEDHISL+LAI++ D  NLPLGWEV+VIFRFF++DQI+DNYLTIQDG
Sbjct: 61  MWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDG 120

Query: 121 KMRKYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 180
           KMRKYSKMK+EHGFT  ++HN F++ASNGFLV+N CTFGVEVS+LKASNKGE LTIF+EP
Sbjct: 121 KMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREP 180

Query: 181 QLGSFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS K N+ Y SEPF VKGRKWR+ IYPNG   GKTSHISLYLKLD+SET
Sbjct: 181 QQDTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSET 240

Query: 241 IPTGKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVF 300
           IP GKKIYAKF L VYNF AKKY+D+S E+WF    +G GF EFLSR ++  H  QND F
Sbjct: 241 IPLGKKIYAKFLLGVYNFSAKKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHS-QNDAF 300

Query: 301 YLKARIVAMSVVQDF 313
           YLKARIVAMS V +F
Sbjct: 301 YLKARIVAMSTVDEF 314

BLAST of CmaCh04G003620 vs. ExPASy TrEMBL
Match: A0A1S3BYG6 (uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494460 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 9.9e-127
Identity = 224/315 (71.11%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEIT-RTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGY 60
           M DNMSYD+ IK E+Q EIT ++ RVDPP++Y FQI+SYS LSEI MKKCESG F VDGY
Sbjct: 1   MDDNMSYDESIKDERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGY 60

Query: 61  NWKLILFPNGDDKVEDHISLYLAITSKD--NLPLGWEVKVIFRFFVYDQIKDNYLTIQDG 120
            WKLIL+PNG+ +VEDHISL+LAI++ D  NLPLGWEV+VIFRFF++DQI+DNYLTIQDG
Sbjct: 61  MWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDG 120

Query: 121 KMRKYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 180
           KMRKYSKMK+EHGFT  ++HN F++ASNGFLV+N CTFGVEVS+LKASNKGE LTIF+EP
Sbjct: 121 KMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREP 180

Query: 181 QLGSFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS K N+ Y SEPF VKGRKWR+ IYPNG   GKTSHISLYLKLD+SET
Sbjct: 181 QQDTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSET 240

Query: 241 IPTGKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVF 300
           IP GKKIYAKF L VYNF AKKY+D+S E+WF    +G GF EFLSR ++  H  QND F
Sbjct: 241 IPLGKKIYAKFLLGVYNFSAKKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHS-QNDAF 300

Query: 301 YLKARIVAMSVVQDF 313
           YLKARIVAMS V +F
Sbjct: 301 YLKARIVAMSTVDEF 314

BLAST of CmaCh04G003620 vs. ExPASy TrEMBL
Match: A0A0A0KUE1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 3.9e-123
Identity = 218/315 (69.21%), Postives = 261/315 (82.86%), Query Frame = 0

Query: 1   MVDNMSYDDPIK-HEQQPEIT-RTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDG 60
           M DNMSYDD  K  E+Q EIT ++ RVDPP++Y FQI+SYS LS+I MKKCESG F VDG
Sbjct: 1   MDDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDG 60

Query: 61  YNWKLILFPNGDDKVEDHISLYLAITSKD-NLPLGWEVKVIFRFFVYDQIKDNYLTIQDG 120
           Y WKLIL+PNG+++VEDHISL+LA+++ D NLPLGWE++VIFRFF++DQI+DNYLTIQDG
Sbjct: 61  YKWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDG 120

Query: 121 KMRKYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP 180
           KMRKYSKMK+EHGFT  ++HN FN+AS+GFLV N CTFGVEVS+LKASNKGE LTI KEP
Sbjct: 121 KMRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEP 180

Query: 181 QLGSFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSET 240
           Q  ++FW++ SFS     FY SEPF VKGRKWR+ +YP+G   GKTSHISLYLKLDSSET
Sbjct: 181 QQDTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 240

Query: 241 IPTGKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVF 300
           IP GKKIYAKF L VYNF AKKY+D+S E+W+    +G+GF EFLSR ++  H  QND F
Sbjct: 241 IPLGKKIYAKFILGVYNFSAKKYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS-QNDAF 300

Query: 301 YLKARIVAMSVVQDF 313
           YLKARIVAMS V++F
Sbjct: 301 YLKARIVAMSTVEEF 314

BLAST of CmaCh04G003620 vs. NCBI nr
Match: XP_022989347.1 (uncharacterized protein LOC111486429 [Cucurbita maxima])

HSP 1 Score: 649.4 bits (1674), Expect = 1.5e-182
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN
Sbjct: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR
Sbjct: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
           KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT
Sbjct: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK
Sbjct: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300

Query: 301 ARIVAMSVVQDF 313
           ARIVAMSVVQDF
Sbjct: 301 ARIVAMSVVQDF 312

BLAST of CmaCh04G003620 vs. NCBI nr
Match: XP_023529004.1 (speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.6 bits (1594), Expect = 2.9e-173
Identity = 295/312 (94.55%), Postives = 304/312 (97.44%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           MVDNMSYDDPIK EQQPEITRT RV+PPANY FQINSYSTLSEIGMKKCESG+FAVDGYN
Sbjct: 1   MVDNMSYDDPIKREQQPEITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGYN 60

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLIL+PNGDD+VEDHISLYLAI SKDNLPLGWEVKVIFRFFVYD+IKDNYLT+QDGKMR
Sbjct: 61  WKLILYPNGDDEVEDHISLYLAIISKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMR 120

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
           KYSKMK+EHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 121 KYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNK NEFY SEPFTVKGRKWRLMIYPNG  SGKTSHISLYLKLDSSET+PT
Sbjct: 181 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKTSHISLYLKLDSSETVPT 240

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK
Sbjct: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300

Query: 301 ARIVAMSVVQDF 313
           ARIVAMSVV+DF
Sbjct: 301 ARIVAMSVVEDF 312

BLAST of CmaCh04G003620 vs. NCBI nr
Match: KAG6600198.1 (Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.8 bits (1566), Expect = 5.1e-170
Identity = 289/312 (92.63%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           M DNMSYDDPIKHEQQPEITRT RV+PPANY FQINSYSTLSEIGMKKCESG+FAVDG+N
Sbjct: 88  MDDNMSYDDPIKHEQQPEITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFN 147

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLIL+PNGDD+VEDHISLYLAITSKDNLPLGWEVKVIFRFFVYD+IKDNYLT+QDGKMR
Sbjct: 148 WKLILYPNGDDEVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMR 207

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
           KYSKMK+EHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 208 KYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 267

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNK NEFY SEPFTVKGRKWRL IYPNG  SGK SHISLYLKLDSSET+PT
Sbjct: 268 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLTIYPNGLSSGKMSHISLYLKLDSSETVPT 327

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVYNFGAKKYVDQSNE+WFDNT YG+GFHEFLSRNDLQPHHLQNDVFYLK
Sbjct: 328 GKKIYAKFFLSVYNFGAKKYVDQSNEHWFDNTTYGYGFHEFLSRNDLQPHHLQNDVFYLK 387

Query: 301 ARIVAMSVVQDF 313
           ARI AMSVV+DF
Sbjct: 388 ARIAAMSVVEDF 399

BLAST of CmaCh04G003620 vs. NCBI nr
Match: XP_022941740.1 (speckle-type POZ protein-like A [Cucurbita moschata])

HSP 1 Score: 604.0 bits (1556), Expect = 7.4e-169
Identity = 286/312 (91.67%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           MVDNMSYDDPIKHEQQPEITRT R +PPANY FQINSYSTLSEIGMKKCESG+FAVDG+N
Sbjct: 1   MVDNMSYDDPIKHEQQPEITRTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFN 60

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLIL+PNGDD+VEDHISLYLAITSKDNLPLGWEVKVIFRFFVYD+IKDNYLT+QDGKMR
Sbjct: 61  WKLILYPNGDDEVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMR 120

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
           KYSKMK+EHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 121 KYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNK NEFY SEPFTVKGRKWRLMIYPNG  SGK SHISLYLKLDSSET+PT
Sbjct: 181 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSETVPT 240

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVY+FGAK+YVDQSNE+WFDNT YG+GFHEFLSRNDL+PHHLQND FYLK
Sbjct: 241 GKKIYAKFFLSVYDFGAKRYVDQSNEHWFDNTTYGYGFHEFLSRNDLRPHHLQNDGFYLK 300

Query: 301 ARIVAMSVVQDF 313
           ARI AMSVV+DF
Sbjct: 301 ARIAAMSVVEDF 312

BLAST of CmaCh04G003620 vs. NCBI nr
Match: KAG7030860.1 (Ubiquitin carboxyl-terminal hydrolase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.6 bits (1394), Expect = 4.5e-150
Identity = 264/312 (84.62%), Postives = 274/312 (87.82%), Query Frame = 0

Query: 1   MVDNMSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYN 60
           M DNMSYDDPIKHEQQPEITRT RV+PPANY FQINSYSTLSEIGMKKCESG+FAVDG+N
Sbjct: 1   MDDNMSYDDPIKHEQQPEITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFN 60

Query: 61  WKLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMR 120
           WKLIL+PNGDD+VEDHISLYLAITSKDNLPLGWE                          
Sbjct: 61  WKLILYPNGDDEVEDHISLYLAITSKDNLPLGWE-------------------------- 120

Query: 121 KYSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180
            YSKMK+EHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG
Sbjct: 121 -YSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLG 180

Query: 181 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 240
           SFFWSISSFSNK NEFY SEPFTVKGRKWRL IYPNG  SGK SHISLYLKLDSSET+PT
Sbjct: 181 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLTIYPNGLSSGKMSHISLYLKLDSSETVPT 240

Query: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLK 300
           GKKIYAKFFLSVYNFGAKKYVDQSNE+WFDNT YG+GFHEFLSRNDLQPHHLQNDVFYLK
Sbjct: 241 GKKIYAKFFLSVYNFGAKKYVDQSNEHWFDNTTYGYGFHEFLSRNDLQPHHLQNDVFYLK 285

Query: 301 ARIVAMSVVQDF 313
           ARI AMSVV+DF
Sbjct: 301 ARIAAMSVVEDF 285

BLAST of CmaCh04G003620 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-50
Identity = 97/270 (35.93%), Postives = 158/270 (58.52%), Query Frame = 0

Query: 18  EITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGD--DKVED 77
           EI ++    PP +Y+ +I S+S L++  +++ E+  F   GY WKL+L+PNG+     +D
Sbjct: 9   EIIKSISDAPPTHYMVKIESFSLLTKHAIERYETESFEAGGYKWKLVLYPNGNKSKNTKD 68

Query: 78  HISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFL 137
           H+S+YL++    +L  GWEV  +FR ++ DQ KDNYL +Q G  R++  +K E GF KF+
Sbjct: 69  HVSVYLSLADSSSLSPGWEVYAVFRLYLLDQNKDNYLILQ-GNERRFHSVKREWGFDKFI 128

Query: 138 AHNAFNQASNGFLVDNGCTFGVEVSVLK--ASNKGESLTIFKEPQLGSFFWSISSFSNKN 197
               F+ ASNG+L+++ C FG +V V K   S +GE L++ K+       W I +FS  +
Sbjct: 129 PTGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSSKHVWKIENFSKLD 188

Query: 198 NEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVY 257
            E Y S  F    RKW++  YP G   G  +H+S+YL L   ETI  G KI+ +F + ++
Sbjct: 189 KESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEFTIRIF 248

Query: 258 NFGAKKYVDQSNEYWFDNTAYGHGFHEFLS 284
           +    +++      WF  ++  HG+ +++S
Sbjct: 249 DQLQGRHIAGKVTKWFSRSSSEHGWVKYVS 277

BLAST of CmaCh04G003620 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 159.1 bits (401), Expect = 5.9e-39
Identity = 92/310 (29.68%), Postives = 153/310 (49.35%), Query Frame = 0

Query: 5   MSYDDPIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLI 64
           + Y+     E+   +TR  R + P++ I  I S+S +   G +  ES  F   GY W+L+
Sbjct: 36  IEYNSSYSLEENLGVTRELREERPSSKIVTITSFSVIKGRG-EPYESSVFEAAGYKWRLV 95

Query: 65  LFPNG--DDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKY 124
           L+ NG  +D   DHISLY  I   ++LPLGWEV V  + FV++     YLT+ DG +++Y
Sbjct: 96  LYVNGNKNDGGNDHISLYARIEETNSLPLGWEVNVDLKLFVHNGKLHKYLTVTDGLVKRY 155

Query: 125 SKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLGSF 184
           +  K E GF + +  + F  A+ G+L  +  +FG E+ ++K + + E +T    P    F
Sbjct: 156 NNAKKEWGFGQLIPRSTFYNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPNNVF 215

Query: 185 FWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGK 244
            W I  FS   ++FY+S+ F V+ R WRL   P G   G+   + ++L     +      
Sbjct: 216 TWKILRFSTLEDKFYYSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVAT 275

Query: 245 KIYAKFFLSVYNFGAKKYVDQSNEYWFD-NTAYGHGFHEFL---SRNDLQPHHLQNDVFY 304
             +    L + N  +  +    +  W+   + YG G +  +     ND    +L ND   
Sbjct: 276 NTWGAVNLRLKNQRSTNHRQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAII 335

Query: 305 LKARIVAMSV 309
            +A +V +S+
Sbjct: 336 FEAEMVKVSI 344

BLAST of CmaCh04G003620 vs. TAIR 10
Match: AT5G26260.1 (TRAF-like family protein )

HSP 1 Score: 154.1 bits (388), Expect = 1.9e-37
Identity = 87/295 (29.49%), Postives = 149/295 (50.51%), Query Frame = 0

Query: 20  TRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGDDK--VEDHI 79
           TR  R + P++ I  I S+S + + G +  ES  F   GY W+L+L+  G+ K  + +HI
Sbjct: 52  TRELRDERPSSKIVTITSFSVIKDRG-EPYESSIFEAAGYKWRLVLYVKGNPKGGINNHI 111

Query: 80  SLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFLAH 139
           SLY  I   + LP GWEV V  + FV+++    YL++ DG +++Y+  K E GFT+ ++ 
Sbjct: 112 SLYARIEETETLPRGWEVNVDLKLFVHNRKLKKYLSVTDGTVKRYNDAKKEWGFTQLISL 171

Query: 140 NAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLGSFFWSISSFSNKNNEFY 199
             F  A+ G+LV +  +FG E+ ++  + K E +T    P    F W I  FS   ++FY
Sbjct: 172 PTFYNANEGYLVQDTASFGAEIFIVNPTEKQEKVTFISNPPDNVFTWKILRFSTLEDKFY 231

Query: 200 FSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVYNFGA 259
           +S+ F V  R WRL   P G   G+   + ++L     +        +    L + N  +
Sbjct: 232 YSDDFLVGDRYWRLGFNPKGSGGGRPHALPIFLYAQGHKANAVVTNTWGAVNLRLKNQRS 291

Query: 260 KKYVDQSNEYWFD-NTAYGHGFHEFLSRNDLQ---PHHLQNDVFYLKARIVAMSV 309
             +    +  W+   + YG G +  +  ++L+     ++ ND    +A +V +SV
Sbjct: 292 SNHKQLYSAAWYPIRSDYGVGVNNIILMSELKDASKGYMVNDAIIFEAEMVKVSV 345

BLAST of CmaCh04G003620 vs. TAIR 10
Match: AT2G15710.1 (TRAF-like family protein )

HSP 1 Score: 151.0 bits (380), Expect = 1.6e-36
Identity = 94/285 (32.98%), Postives = 148/285 (51.93%), Query Frame = 0

Query: 19  ITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGD--DKVEDH 78
           I    R  PP++Y  +IN    LS++   K ES  F   GYNW+L+++P G+  DK    
Sbjct: 89  IVNNLREHPPSSYSLKINK---LSQLTFDKYESHRFLSGGYNWRLVIYPKGNEKDKGSGF 148

Query: 79  ISLYLAI-TSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFL 138
           IS+Y+    +K +     EV     FFVY++ ++ Y TIQD ++++++ ++T  G ++ L
Sbjct: 149 ISMYVEFDNTKVSSTSPMEVFAYIIFFVYNKKENKYFTIQDVEVKRFNALRTVWGLSQVL 208

Query: 139 AHNAFNQASNGFLVD-NGCTFGVEVSVLKASNKGESLTIFKEPQLGSFFWSISSFSNKNN 198
           +   FN   NG+  +   C FGV+V V     K E ++  ++  +  F WS+  FS    
Sbjct: 209 SLETFNDLENGYTFEGEQCEFGVDVMVASPITKWEVVSFDEKLDILKFSWSVKDFSVLKE 268

Query: 199 EFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVYN 258
           EFY SE F++ GR W L +YP G        +S++L+L  SET+   +KIY    L V  
Sbjct: 269 EFYVSERFSMGGRLWDLQMYPKGDPRRDKKWLSIFLRLSGSETLTVDEKIYVIAHLRV-- 328

Query: 259 FGAKKYVDQSNEYWFDNTAYGHGFHEFLSRNDLQPHHLQ-NDVFY 299
                 +D     WF +   G G+ EFLS + L+  +L   D F+
Sbjct: 329 ------LDPLGN-WFRDRNKGWGYLEFLSFSKLRKSYLDLEDTFF 361

BLAST of CmaCh04G003620 vs. TAIR 10
Match: AT2G04170.1 (TRAF-like family protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.0e-35
Identity = 89/291 (30.58%), Postives = 142/291 (48.80%), Query Frame = 0

Query: 24  RVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNG--DDKVEDHISLYL 83
           R + P+N I  I S+S +   G +  ES  F   GY W+L+L+ NG  +D   +HISLY+
Sbjct: 125 RDERPSNKILTITSFSVIKGRG-EPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHISLYV 184

Query: 84  AITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFLAHNAFN 143
            I   ++LP GWEV V  + FVY+  +  YL ++DG +++Y+  K E G+ K +    F 
Sbjct: 185 RIEETESLPKGWEVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGKLIPLTTFL 244

Query: 144 QASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLGSFFWSISSFSNKNNEFYFSEP 203
             + G+L  +  +FG E+    A    E +T    P    F W I  FSN  ++FY+S+ 
Sbjct: 245 DTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLEDKFYYSDD 304

Query: 204 FTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVYNFGAKKYV 263
           F V+ R WRL   P G   G++  I ++L     +        +    L + N  +  + 
Sbjct: 305 FLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLKNQRSSNHA 364

Query: 264 DQSNEYWFDNTA-YGHGFHEFLSR---NDLQPHHLQNDVFYLKARIVAMSV 309
              +  W+   + YG G +  +S    ND    +  ND    +A +V +SV
Sbjct: 365 QIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSV 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WU21.2e-1229.50Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 ... [more]
Q9FPT11.6e-1229.87Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 ... [more]
Q9M2I93.9e-1133.06MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis t... [more]
Q9SHT31.2e-0931.78MATH domain and coiled-coil domain-containing protein At2g05410 OS=Arabidopsis t... [more]
Q9M2H98.1e-0928.24MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A6J1JFK17.5e-183100.00uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429... [more]
A0A6J1FUL43.6e-16991.67speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE... [more]
A0A5A7TNH79.9e-12771.11Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. ma... [more]
A0A1S3BYG69.9e-12771.11uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KUE13.9e-12369.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022989347.11.5e-182100.00uncharacterized protein LOC111486429 [Cucurbita maxima][more]
XP_023529004.12.9e-17394.55speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo][more]
KAG6600198.15.1e-17092.63Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp.... [more]
XP_022941740.17.4e-16991.67speckle-type POZ protein-like A [Cucurbita moschata][more]
KAG7030860.14.5e-15084.62Ubiquitin carboxyl-terminal hydrolase 12 [Cucurbita argyrosperma subsp. argyrosp... [more]
Match NameE-valueIdentityDescription
AT3G17380.11.1e-5035.93TRAF-like family protein [more]
AT5G26280.15.9e-3929.68TRAF-like family protein [more]
AT5G26260.11.9e-3729.49TRAF-like family protein [more]
AT2G15710.11.6e-3632.98TRAF-like family protein [more]
AT2G04170.11.0e-3530.58TRAF-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002083MATH/TRAF domainSMARTSM00061math_3coord: 33..140
e-value: 0.093
score: 14.9
coord: 181..287
e-value: 6.6E-11
score: 52.2
IPR002083MATH/TRAF domainPFAMPF00917MATHcoord: 186..303
e-value: 1.8E-13
score: 50.7
coord: 39..161
e-value: 9.2E-10
score: 38.7
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 28..159
score: 24.507631
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 179..303
score: 27.166859
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 192..303
e-value: 2.27414E-21
score: 85.8938
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 31..159
e-value: 5.92051E-22
score: 87.4346
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 174..308
e-value: 1.2E-26
score: 95.4
coord: 12..168
e-value: 5.6E-36
score: 125.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR46162:SF29SUBFAMILY NOT NAMEDcoord: 14..311
NoneNo IPR availablePANTHERPTHR46162TRAF-LIKE FAMILY PROTEINcoord: 14..311
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 180..308
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 29..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G003620.1CmaCh04G003620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0005515 protein binding