CmaCh04G002430 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G002430
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description3-ketoacyl-CoA synthase
LocationCma_Chr04: 1183822 .. 1185231 (-)
RNA-Seq ExpressionCmaCh04G002430
SyntenyCmaCh04G002430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGATTTTCATGGTAGTATGTTTACTGTCTCTTTTCTATTCTTTCTTCTGCATTTGGAAGTTGATTCTGCAAAGGAGAGACCAATGCTGTTATCTGTTGGGGTACGAGTGCTATAAAGCTACTGAAGACAGGAAGCTGGGTGTGGAAGCCTGTGTAAATATCATCTTGAGGAACAGGAATCTGGGGCTTGAAGAATACCGATACCTACTCAAAAGCATGGTCAATTCGGGATTAGGAGGAGAAACTTACGGCCCGAAAAACATAATTGCAGCCAGAGAAGAAAGCCCTTCTCTATCAGATGCTATTTCGGAGATGGATGATGTGTTTTCGAGCGCACTCGACAAACTCTTTGCCAAGACGGGAGTTTCTCCATCTGACATTGATATACTTGTTGTGAATGTGTCTTTGTTCTCTCCAGCGCCCTCGTTGACTGCTCGAATAATAAACCGTTACAAGATGAGGGAGGATGTCAAAGCCTTCAACCTCTCTGGGATGGGGTGCAGTGCCAGTATTGTAGCTATTGATCTCGTGAAACACTTGTTCAAGACAAACAAGAATGCATATGCGGTTGTTGTTAGTACAGAATCCATAGGACCAAACTGGTATAGTGGGAAGGAACAGCCTATGATGCTCACGAACTGTTTGTACCGATCGGGAGGCTGTTCGATGCTCTTCACGAACAAGGCAGCTTTGAAGGATTCTGCTCTGCTGAAACTTAAATGTATTCTTCGAACACATTTGGGATCAAGCAACGAAGCATATGGATGTTCCATTCAAGTGGAAGATGACCATGGTCACCGAGGTTTTCGTCTCACCAAACACATAAAAGCTGTTGCAACTCAAGCTCTCACCATCAACCTTCAAACTCTACTGCCTAGGATTTTACCATTGAGGGAACTACTTCGCTATGCCATCCTAACTCATCCTCTTTACAAAACCATCAACGGCAACATCGAAGCAAAGAAAGTGCGCTTGAATCTGAAGACTGGAGTCGATCACTTTGGGATACCACCAACTGAAAGGGCAGTCATTGATGGGATCGGTAAGAGCTTAGGGCTGAGTGATTATGACCTAGAGCCTGCAAGAATGGCACTCCATAGATTTGGAAACACATCCACTGGTGGCCTTTGGTATGTTTTAGGCTACATGGAAGCCAAAAAAAGGCTAAAAGAGGGTGACAAGATTCTGATGATAAGCTTTGGAGCAGGCTATGAATGCAACAGCTGTGTGTGGGAGGTTATGAGCAACTTGGAAGATGAAAATGTCTGGAAAGACTGCATCCATAGCTACCCTACAAAATACACTGTGAATCTACTTAAAGAGAAATGCAGTTGGCTCAACGACGAGCGTCTATCGTTCGTTAAGTTTGACGACGTAAAACACAATCTACAGCGACAAATTCTTTGA

mRNA sequence

ATGGGGATTTTCATGGTAGTATGTTTACTGTCTCTTTTCTATTCTTTCTTCTGCATTTGGAAGTTGATTCTGCAAAGGAGAGACCAATGCTGTTATCTGTTGGGGTACGAGTGCTATAAAGCTACTGAAGACAGGAAGCTGGGTGTGGAAGCCTGTGTAAATATCATCTTGAGGAACAGGAATCTGGGGCTTGAAGAATACCGATACCTACTCAAAAGCATGGTCAATTCGGGATTAGGAGGAGAAACTTACGGCCCGAAAAACATAATTGCAGCCAGAGAAGAAAGCCCTTCTCTATCAGATGCTATTTCGGAGATGGATGATGTGTTTTCGAGCGCACTCGACAAACTCTTTGCCAAGACGGGAGTTTCTCCATCTGACATTGATATACTTGTTGTGAATGTGTCTTTGTTCTCTCCAGCGCCCTCGTTGACTGCTCGAATAATAAACCGTTACAAGATGAGGGAGGATGTCAAAGCCTTCAACCTCTCTGGGATGGGGTGCAGTGCCAGTATTGTAGCTATTGATCTCGTGAAACACTTGTTCAAGACAAACAAGAATGCATATGCGGTTGTTGTTAGTACAGAATCCATAGGACCAAACTGGTATAGTGGGAAGGAACAGCCTATGATGCTCACGAACTGTTTGTACCGATCGGGAGGCTGTTCGATGCTCTTCACGAACAAGGCAGCTTTGAAGGATTCTGCTCTGCTGAAACTTAAATGTATTCTTCGAACACATTTGGGATCAAGCAACGAAGCATATGGATGTTCCATTCAAGTGGAAGATGACCATGGTCACCGAGGTTTTCGTCTCACCAAACACATAAAAGCTGTTGCAACTCAAGCTCTCACCATCAACCTTCAAACTCTACTGCCTAGGATTTTACCATTGAGGGAACTACTTCGCTATGCCATCCTAACTCATCCTCTTTACAAAACCATCAACGGCAACATCGAAGCAAAGAAAGTGCGCTTGAATCTGAAGACTGGAGTCGATCACTTTGGGATACCACCAACTGAAAGGGCAGTCATTGATGGGATCGGTAAGAGCTTAGGGCTGAGTGATTATGACCTAGAGCCTGCAAGAATGGCACTCCATAGATTTGGAAACACATCCACTGGTGGCCTTTGGTATGTTTTAGGCTACATGGAAGCCAAAAAAAGGCTAAAAGAGGGTGACAAGATTCTGATGATAAGCTTTGGAGCAGGCTATGAATGCAACAGCTGTGTGTGGGAGGTTATGAGCAACTTGGAAGATGAAAATGTCTGGAAAGACTGCATCCATAGCTACCCTACAAAATACACTGTGAATCTACTTAAAGAGAAATGCAGTTGGCTCAACGACGAGCGTCTATCGTTCGTTAAGTTTGACGACGTAAAACACAATCTACAGCGACAAATTCTTTGA

Coding sequence (CDS)

ATGGGGATTTTCATGGTAGTATGTTTACTGTCTCTTTTCTATTCTTTCTTCTGCATTTGGAAGTTGATTCTGCAAAGGAGAGACCAATGCTGTTATCTGTTGGGGTACGAGTGCTATAAAGCTACTGAAGACAGGAAGCTGGGTGTGGAAGCCTGTGTAAATATCATCTTGAGGAACAGGAATCTGGGGCTTGAAGAATACCGATACCTACTCAAAAGCATGGTCAATTCGGGATTAGGAGGAGAAACTTACGGCCCGAAAAACATAATTGCAGCCAGAGAAGAAAGCCCTTCTCTATCAGATGCTATTTCGGAGATGGATGATGTGTTTTCGAGCGCACTCGACAAACTCTTTGCCAAGACGGGAGTTTCTCCATCTGACATTGATATACTTGTTGTGAATGTGTCTTTGTTCTCTCCAGCGCCCTCGTTGACTGCTCGAATAATAAACCGTTACAAGATGAGGGAGGATGTCAAAGCCTTCAACCTCTCTGGGATGGGGTGCAGTGCCAGTATTGTAGCTATTGATCTCGTGAAACACTTGTTCAAGACAAACAAGAATGCATATGCGGTTGTTGTTAGTACAGAATCCATAGGACCAAACTGGTATAGTGGGAAGGAACAGCCTATGATGCTCACGAACTGTTTGTACCGATCGGGAGGCTGTTCGATGCTCTTCACGAACAAGGCAGCTTTGAAGGATTCTGCTCTGCTGAAACTTAAATGTATTCTTCGAACACATTTGGGATCAAGCAACGAAGCATATGGATGTTCCATTCAAGTGGAAGATGACCATGGTCACCGAGGTTTTCGTCTCACCAAACACATAAAAGCTGTTGCAACTCAAGCTCTCACCATCAACCTTCAAACTCTACTGCCTAGGATTTTACCATTGAGGGAACTACTTCGCTATGCCATCCTAACTCATCCTCTTTACAAAACCATCAACGGCAACATCGAAGCAAAGAAAGTGCGCTTGAATCTGAAGACTGGAGTCGATCACTTTGGGATACCACCAACTGAAAGGGCAGTCATTGATGGGATCGGTAAGAGCTTAGGGCTGAGTGATTATGACCTAGAGCCTGCAAGAATGGCACTCCATAGATTTGGAAACACATCCACTGGTGGCCTTTGGTATGTTTTAGGCTACATGGAAGCCAAAAAAAGGCTAAAAGAGGGTGACAAGATTCTGATGATAAGCTTTGGAGCAGGCTATGAATGCAACAGCTGTGTGTGGGAGGTTATGAGCAACTTGGAAGATGAAAATGTCTGGAAAGACTGCATCCATAGCTACCCTACAAAATACACTGTGAATCTACTTAAAGAGAAATGCAGTTGGCTCAACGACGAGCGTCTATCGTTCGTTAAGTTTGACGACGTAAAACACAATCTACAGCGACAAATTCTTTGA

Protein sequence

MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRELLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLEDENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL
Homology
BLAST of CmaCh04G002430 vs. ExPASy Swiss-Prot
Match: Q9LZ72 (3-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.7e-157
Identity = 270/464 (58.19%), Postives = 362/464 (78.02%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSF-FCIWKLILQRRDQ-CCYLLGYECYKATEDRKLGVEACVNIILR 60
           M +F +  LL L   F F I+K + +RR+Q  CY+L YECYK  E+RKL  E C  ++ R
Sbjct: 1   MELFSLSSLLLLSTLFVFYIFKFVFKRRNQRNCYMLHYECYKGMEERKLDTETCAKVVQR 60

Query: 61  NRNLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLF 120
           N+NLGLEEYR+LL++M +SG+G ETYGP+N++  RE+SP+L DA SEMD++    LDKLF
Sbjct: 61  NKNLGLEEYRFLLRTMASSGIGEETYGPRNVLEGREDSPTLLDAHSEMDEIMFDTLDKLF 120

Query: 121 AKT--GVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAID 180
            KT   +SPSDIDILVVNVSLF+P+PSLT+R+INRYKMRED+K++NLSG+GCSAS+++ID
Sbjct: 121 HKTKGSISPSDIDILVVNVSLFAPSPSLTSRVINRYKMREDIKSYNLSGLGCSASVISID 180

Query: 181 LVKHLFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSA 240
           +V+ +F+T +NA A+VVSTE++GP+WY GK++ MML+NCL+R+GG S+L TN A  K+ A
Sbjct: 181 IVQRMFETRENALALVVSTETMGPHWYCGKDRSMMLSNCLFRAGGSSVLLTNAARFKNQA 240

Query: 241 LLKLKCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRIL 300
           L+KL  ++R H+GS +EAY C IQ+ED  GH GF LTK++K  A +ALT NLQ LLPR+L
Sbjct: 241 LMKLVTVVRAHVGSDDEAYSCCIQMEDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVL 300

Query: 301 PLRELLRYAILTHPLYKTINGNIEAKK-VRLNLKTGVDHFGIPPTERAVIDGIGKSLGLS 360
           P++EL+RYAI+     +T      A   + LNLKTG+ HF I P  RA+I+G+GKSLGL+
Sbjct: 301 PVKELIRYAIVRALKRRTSAKREPASSGIGLNLKTGLQHFCIHPGGRAIIEGVGKSLGLT 360

Query: 361 DYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVM 420
           ++D+EPARMALHRFGNTS+GGLWYVLGYMEAK RLK+G+KILM+S GAG+E N+CVWEV+
Sbjct: 361 EFDIEPARMALHRFGNTSSGGLWYVLGYMEAKNRLKKGEKILMMSMGAGFESNNCVWEVL 420

Query: 421 SNLEDENVWKDCIHSYPTKYTV-NLLKEKCSWLNDERLSFVKFD 459
            +L+D+NVW+D +  YP    + N   EK  W+ND+ +SFV+ D
Sbjct: 421 KDLDDKNVWEDSVDRYPELSRIPNPFVEKYDWINDDTMSFVRVD 464

BLAST of CmaCh04G002430 vs. ExPASy Swiss-Prot
Match: Q9SIB2 (3-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 4.4e-136
Identity = 240/475 (50.53%), Postives = 323/475 (68.00%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           M +  +   L L Y FF IWKLI  ++D+ CY+L Y+C+K T+DR +  +    II RN+
Sbjct: 1   MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQ 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGL EY++LLK++V+SG+G +TY P+ +   REE PSL D ISEM++ +  ++ KL  +
Sbjct: 61  NLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLER 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
             +SP DIDILVVNVS+ S  PSL +RIIN YKMR+DVK FNL+GMGCSAS++++D+VK+
Sbjct: 121 NQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKN 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           +FK+  N  A+V ++ES+ PNWYSG  + M+L NCL+RSGGC++L TNK +L+  A+ KL
Sbjct: 181 IFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KC++RTH G+  E+Y C IQ ED+ G  GF L K++   AT+A   NL+ + P+ILP+ E
Sbjct: 241 KCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTE 300

Query: 301 LLRYAILTHPLYKTI-------NGNIEAK-----KVRLNLKTGVDHFGIPPTERAVIDGI 360
           L+R+ +    L K I        G+         K  +N KTG++HF I    +AVIDGI
Sbjct: 301 LIRFMLKL--LIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGI 360

Query: 361 GKSLGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECN 420
           G SL L++YD+EPARM LHRFGNTS   LWYVL YMEAKKRLK GD++ MISFGAG++CN
Sbjct: 361 GHSLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCN 420

Query: 421 SCVWEVMSNL----EDENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDD 460
           SCVWEV+ +L       NVW  CI  YP K  +N   EK  W+ DE     K  D
Sbjct: 421 SCVWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFKVPD 473

BLAST of CmaCh04G002430 vs. ExPASy Swiss-Prot
Match: Q9LQP8 (3-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3)

HSP 1 Score: 476.1 bits (1224), Expect = 4.6e-133
Identity = 233/463 (50.32%), Postives = 325/463 (70.19%), Query Frame = 0

Query: 5   MVVCLLSLFYSF--FCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNL 64
           ++V LLSL  S+  F IWK I  +RDQ CY+L Y+C+K ++DR +  +   +IILRN++L
Sbjct: 3   LLVMLLSLLVSYLIFKIWKRIDSKRDQNCYILDYQCHKPSDDRMVNTQFSGDIILRNKHL 62

Query: 65  GLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTG 124
            L EY++LLK++V+SG+G +TY P+     RE+ P+L D +SEM++ +   ++K+  +  
Sbjct: 63  RLNEYKFLLKAIVSSGIGEQTYAPRLFFEGREQRPTLQDGLSEMEEFYIDTIEKVLKRNK 122

Query: 125 VSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLF 184
           +SPS+IDILVVNVS+ +  PSL+ARIIN YKMRED+K FNL+ MGCSAS+++ID+VK++F
Sbjct: 123 ISPSEIDILVVNVSMLNSTPSLSARIINHYKMREDIKVFNLTAMGCSASVISIDIVKNIF 182

Query: 185 KTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKC 244
           KT KN  A+VV++ES+ PNWYSG  + M+L NCL+RSGGC++L TNK +L   A+ KL+C
Sbjct: 183 KTYKNKLALVVTSESLSPNWYSGNNRSMILANCLFRSGGCAVLLTNKRSLSRRAMFKLRC 242

Query: 245 ILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRELL 304
           ++RTH G+ ++++   +Q ED+ GH G  L K +   AT+A   NL+ + P+ILP+ ELL
Sbjct: 243 LVRTHHGARDDSFNACVQKEDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTELL 302

Query: 305 RYAIL-------THPLYKTINGNIEAK----KVRLNLKTGVDHFGIPPTERAVIDGIGKS 364
           R+ +        + P   + N    A     K  +N KTG+DHF I    +AVID IG S
Sbjct: 303 RFMLCLLLKKLRSSPSKGSTNVTQAAPKAGVKAGINFKTGIDHFCIHTGGKAVIDAIGYS 362

Query: 365 LGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCV 424
           L L++YDLEPARM LHRFGNTS   LWYVLGYMEAKKRLK GD++ MISFGAG++CNSCV
Sbjct: 363 LDLNEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKRGDRVFMISFGAGFKCNSCV 422

Query: 425 WEVMSNLE----DENVWKDCIHSYPTKYTVNLLKEKCSWLNDE 451
           WEV+ +L       NVW  CI+ YP K  +N   EK  W+++E
Sbjct: 423 WEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEE 465

BLAST of CmaCh04G002430 vs. ExPASy Swiss-Prot
Match: Q9MAM3 (3-ketoacyl-CoA synthase 1 OS=Arabidopsis thaliana OX=3702 GN=KCS1 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-92
Identity = 177/433 (40.88%), Postives = 266/433 (61.43%), Query Frame = 0

Query: 6   VVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNLGLE 65
           + CL  +F SF  +  L +  R +  YL+ + CYK  ++RK+ V++ + +   N +   +
Sbjct: 101 LTCL--VFLSF--VLTLYVANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDD 160

Query: 66  EYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTGVSP 125
             ++  +    +GLG ETY P+ I +   +  ++S+A +E + V   ALD LF KTG+ P
Sbjct: 161 TVQFQQRISNRAGLGDETYLPRGITSTPPKL-NMSEARAEAEAVMFGALDSLFEKTGIKP 220

Query: 126 SDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLFKTN 185
           +++ IL+VN SLF+P PSL+A I+N YKMRED+K++NL GMGCSA +++IDL  +L K N
Sbjct: 221 AEVGILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKAN 280

Query: 186 KNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKCILR 245
            N+YAVVVSTE+I  NWY G ++ M+L NC++R GG ++L +N+   +  +   L  ++R
Sbjct: 281 PNSYAVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVR 340

Query: 246 THLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRELLRYA 305
           TH GS ++ Y C  Q ED+ G  G  L + + +VA  AL  N+ TL P +LPL E L + 
Sbjct: 341 THKGSDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFL 400

Query: 306 ILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLEPARMA 365
           I    L K     ++ K    + K   +HF I    RAV+D + K+L L D+ +EP+RM 
Sbjct: 401 I---SLVKRKMFKLKVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMT 460

Query: 366 LHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLEDE---- 425
           LHRFGNTS+  LWY + Y EAK R+K GD++  I+FG+G++CNS VW+ +  +  E    
Sbjct: 461 LHRFGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTG 520

Query: 426 NVWKDCIHSYPTK 435
           N W   I  YP K
Sbjct: 521 NAWAGSIDQYPVK 525

BLAST of CmaCh04G002430 vs. ExPASy Swiss-Prot
Match: Q9ZUZ0 (3-ketoacyl-CoA synthase 13 OS=Arabidopsis thaliana OX=3702 GN=HIC PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 5.0e-87
Identity = 164/415 (39.52%), Postives = 254/415 (61.20%), Query Frame = 0

Query: 27  RDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNLGLEEYRYLLKSMVNSGLGGETYGP 86
           R +  YL+ + C++ T+  K+  E   N+    +    E  +++ + +  SGLG +TY P
Sbjct: 56  RSKPVYLVDFSCHQPTDSCKISSETFFNMAKGAQLYTDETIQFMTRILNRSGLGDDTYSP 115

Query: 87  KNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTGVSPSDIDILVVNVSLFSPAPSLTA 146
           + ++ +   +PS+ +A  E + V   AL+ LF KTG+ P ++ I +VN SLF+P PSL++
Sbjct: 116 RCMLTS-PPTPSMYEARHESELVIFGALNSLFKKTGIEPREVGIFIVNCSLFNPNPSLSS 175

Query: 147 RIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLFKTNKNAYAVVVSTESIGPNWYSGK 206
            I+NRYK++ DVK +NLSGMGCSA  +++DL  +L K N N YAV+VSTE++  + Y G 
Sbjct: 176 MIVNRYKLKTDVKTYNLSGMGCSAGAISVDLATNLLKANPNTYAVIVSTENMTLSMYRGN 235

Query: 207 EQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKCILRTHLGSSNEAYGCSIQVEDDHG 266
           ++ M++ NCL+R GG +++ +N++  +  +  +L  I+RTH GSS++ Y C+ Q ED  G
Sbjct: 236 DRSMLVPNCLFRVGGAAVMLSNRSQDRVRSKYELTHIVRTHKGSSDKHYTCAEQKEDSKG 295

Query: 267 HRGFRLTKHIKAVATQALTINLQTLLPRILPLRELLRYAILTHPLYKTINGNIEAKKVRL 326
             G  L+K +  VA  +L  NL  L P +LPL E LR+ +    L K+    ++      
Sbjct: 296 IVGVALSKELTVVAGDSLKTNLTALGPLVLPLSEKLRFILF---LVKSKLFRLKVSPYVP 355

Query: 327 NLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEA 386
           + K    HF I    RA++D + K LGLS++DLEP+RM LHRFGNTS+  LWY L Y+EA
Sbjct: 356 DFKLCFKHFCIHAGGRALLDAVEKGLGLSEFDLEPSRMTLHRFGNTSSSSLWYELAYVEA 415

Query: 387 KKRLKEGDKILMISFGAGYECNSCVWEVMSNLEDE-----NVWKDCIHSYPTKYT 437
           K R+K GD++  ++FG+G++CNS VW  +  +        N W D +H YP   T
Sbjct: 416 KCRVKRGDRVWQLAFGSGFKCNSIVWRALRTIPANESLVGNPWGDSVHKYPVHVT 466

BLAST of CmaCh04G002430 vs. ExPASy TrEMBL
Match: A0A6J1JLQ7 (3-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111488046 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 6.8e-273
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL
Sbjct: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 469

BLAST of CmaCh04G002430 vs. ExPASy TrEMBL
Match: A0A6J1FLF1 (3-ketoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC111446876 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 9.2e-270
Identity = 464/469 (98.93%), Postives = 464/469 (98.93%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAILTHP YKTINGNIE KKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 301 LLRYAILTHPFYKTINGNIEEKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYP KYTVN L EKCSWLNDERLSFVKFDDVKHNLQRQIL
Sbjct: 421 ENVWKDCIHSYPAKYTVNPLAEKCSWLNDERLSFVKFDDVKHNLQRQIL 469

BLAST of CmaCh04G002430 vs. ExPASy TrEMBL
Match: A0A0A0KNS7 (3-ketoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_5G178710 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.9e-248
Identity = 423/469 (90.19%), Postives = 447/469 (95.31%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           M IF+ VCLLSLFY FF IWKLILQRR QCCYLLGYECYKA+EDRKLG+E+C+NIILRN 
Sbjct: 1   MEIFVPVCLLSLFYVFFYIWKLILQRRGQCCYLLGYECYKASEDRKLGIESCINIILRNN 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKS+VNSGLGGETYGP+N+IA  EE+PSLS+AISEMDDVFSS LDKLFAK
Sbjct: 61  NLGLEEYRYLLKSIVNSGLGGETYGPRNVIAGTEENPSLSEAISEMDDVFSSILDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKM+ED+KAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMKEDIKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTN+NAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSML TN AALK SALLKL
Sbjct: 181 LFKTNRNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLLTNNAALKHSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALT NLQ+LLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTFNLQSLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           L+RYAILTHPLYK ++ NIEAKKVRLNLKTGVDHFGIPPTERAVIDG+GKSLGLSDYDLE
Sbjct: 301 LIRYAILTHPLYKLVDRNIEAKKVRLNLKTGVDHFGIPPTERAVIDGLGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLK+GDK+LMISFGAGYECNSCVWEVMSNL+D
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKKGDKVLMISFGAGYECNSCVWEVMSNLKD 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           +NVWKDCI SYPTK T + L EKCSWLNDERLSFVKFDDVK  LQ+QIL
Sbjct: 421 DNVWKDCILSYPTKNTKHPLAEKCSWLNDERLSFVKFDDVKQTLQQQIL 469

BLAST of CmaCh04G002430 vs. ExPASy TrEMBL
Match: A0A6J1CD51 (3-ketoacyl-CoA synthase OS=Momordica charantia OX=3673 GN=LOC111010111 PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.2e-247
Identity = 418/469 (89.13%), Postives = 445/469 (94.88%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           M +FM +CLLS+FY+FFCIWKLIL+RRDQCCYLLGYECYKATEDRKL +E+CVNIILRN+
Sbjct: 53  MEVFMAICLLSVFYAFFCIWKLILRRRDQCCYLLGYECYKATEDRKLRMESCVNIILRNK 112

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLL+SMVNSGLG ETYGP+N+IA REE PSLSDAISEMDDVFSS LD+LFAK
Sbjct: 113 NLGLEEYRYLLRSMVNSGLGSETYGPRNVIAGREECPSLSDAISEMDDVFSSTLDELFAK 172

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDIL+VNVSLFSP PSLTA +INRYKM+ED+KAFNLSGMGCSASIVAIDLVKH
Sbjct: 173 TGVSPSDIDILIVNVSLFSPVPSLTAWVINRYKMKEDIKAFNLSGMGCSASIVAIDLVKH 232

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTN+NAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSML TN AALK SALLKL
Sbjct: 233 LFKTNRNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLLTNNAALKHSALLKL 292

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQ LLPRILPLRE
Sbjct: 293 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQRLLPRILPLRE 352

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRY ILTHPL K IN NIEAKKVRLNLK+GVDHFGIPPTERAVIDG+G+SLGLSDYDLE
Sbjct: 353 LLRYTILTHPLCKVINPNIEAKKVRLNLKSGVDHFGIPPTERAVIDGLGRSLGLSDYDLE 412

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLK+G+KI+MISFGAGYECNSCVWEVMSNL+D
Sbjct: 413 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKKGNKIMMISFGAGYECNSCVWEVMSNLKD 472

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCI SYP K TVN L EKC W+NDERLSFVKFDD+K NLQ+QIL
Sbjct: 473 ENVWKDCIQSYPAKTTVNPLAEKCGWINDERLSFVKFDDIKRNLQQQIL 521

BLAST of CmaCh04G002430 vs. ExPASy TrEMBL
Match: A0A1S3CF49 (3-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103500178 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.4e-247
Identity = 420/469 (89.55%), Postives = 446/469 (95.10%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           M IF+ +CLLSLFY FF IWKLILQRR QCCYLLGYECYK TEDRKLGVE+C+NIILRN+
Sbjct: 1   MEIFVPICLLSLFYVFFYIWKLILQRRGQCCYLLGYECYKTTEDRKLGVESCINIILRNK 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKS+VNSGLGGETYGP+N+IA REE+PSLS+AI EMDDVFSS LDKLFAK
Sbjct: 61  NLGLEEYRYLLKSIVNSGLGGETYGPRNVIAGREENPSLSEAILEMDDVFSSILDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKM+ED+KAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMKEDIKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTN+NAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSML TN +ALK SALLKL
Sbjct: 181 LFKTNRNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLLTNNSALKHSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALT NLQ+LLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTFNLQSLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           L+RY+ILTHPLYK ++ NIEAKKVRLNLKTGVDHFGIPPTERAVIDG+GKSLGLSDYDLE
Sbjct: 301 LIRYSILTHPLYKLVDKNIEAKKVRLNLKTGVDHFGIPPTERAVIDGLGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLK+GDK+LMISFGAGYECNSCVWEVMSNL+D
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKKGDKVLMISFGAGYECNSCVWEVMSNLKD 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           +NVWKDCI +YPTK T + L EKC WLNDERLSFVKFDDVK  LQRQIL
Sbjct: 421 DNVWKDCILNYPTKNTKHPLAEKCGWLNDERLSFVKFDDVKQTLQRQIL 469

BLAST of CmaCh04G002430 vs. NCBI nr
Match: XP_022991407.1 (3-ketoacyl-CoA synthase 19-like [Cucurbita maxima])

HSP 1 Score: 949.1 bits (2452), Expect = 1.4e-272
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL
Sbjct: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 469

BLAST of CmaCh04G002430 vs. NCBI nr
Match: XP_023554275.1 (3-ketoacyl-CoA synthase 19-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.4 bits (2432), Expect = 2.9e-270
Identity = 465/469 (99.15%), Postives = 466/469 (99.36%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAILTHP YKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 301 LLRYAILTHPFYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYP K+TVN LKEKCSWLNDERLSFVKFDDVKHNLQRQIL
Sbjct: 421 ENVWKDCIHSYPAKFTVNPLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 469

BLAST of CmaCh04G002430 vs. NCBI nr
Match: XP_022941551.1 (3-ketoacyl-CoA synthase 19-like [Cucurbita moschata])

HSP 1 Score: 938.7 bits (2425), Expect = 1.9e-269
Identity = 464/469 (98.93%), Postives = 464/469 (98.93%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAILTHP YKTINGNIE KKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 301 LLRYAILTHPFYKTINGNIEEKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYP KYTVN L EKCSWLNDERLSFVKFDDVKHNLQRQIL
Sbjct: 421 ENVWKDCIHSYPAKYTVNPLAEKCSWLNDERLSFVKFDDVKHNLQRQIL 469

BLAST of CmaCh04G002430 vs. NCBI nr
Match: KAG7030748.1 (3-ketoacyl-CoA synthase 19 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 934.9 bits (2415), Expect = 2.8e-268
Identity = 462/469 (98.51%), Postives = 463/469 (98.72%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 26  MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 85

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 86  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 145

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 146 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 205

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 206 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 265

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 266 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 325

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAILTHP YKTINGNIE KKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 326 LLRYAILTHPFYKTINGNIEEKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 385

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PAR ALHRFGNTSTGGLWYVLGYMEAKKRLK+GDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 386 PARTALHRFGNTSTGGLWYVLGYMEAKKRLKKGDKILMISFGAGYECNSCVWEVMSNLED 445

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYP KYTVN L EKCSWLNDERLSFVKFDDVKHNLQRQIL
Sbjct: 446 ENVWKDCIHSYPAKYTVNPLAEKCSWLNDERLSFVKFDDVKHNLQRQIL 494

BLAST of CmaCh04G002430 vs. NCBI nr
Match: KAG6600079.1 (3-ketoacyl-CoA synthase 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 930.6 bits (2404), Expect = 5.2e-267
Identity = 460/469 (98.08%), Postives = 462/469 (98.51%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR
Sbjct: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK
Sbjct: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
           TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH
Sbjct: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL
Sbjct: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE
Sbjct: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300

Query: 301 LLRYAILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360
           LLRYAI THP YKTINGNIE KKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE
Sbjct: 301 LLRYAIPTHPFYKTINGNIEEKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLE 360

Query: 361 PARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLED 420
           PAR ALHRFGNTSTGGLWYVLGYMEAKKRLK+GDKILMISFGAGYECNSCVWEVMSNLED
Sbjct: 361 PARTALHRFGNTSTGGLWYVLGYMEAKKRLKKGDKILMISFGAGYECNSCVWEVMSNLED 420

Query: 421 ENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDDVKHNLQRQIL 470
           ENVWKDCIHSYP KYTVN L EKCSWLNDERLSFVKFDDVKHNLQ+QIL
Sbjct: 421 ENVWKDCIHSYPAKYTVNPLAEKCSWLNDERLSFVKFDDVKHNLQQQIL 469

BLAST of CmaCh04G002430 vs. TAIR 10
Match: AT5G04530.1 (3-ketoacyl-CoA synthase 19 )

HSP 1 Score: 556.6 bits (1433), Expect = 1.9e-158
Identity = 270/464 (58.19%), Postives = 362/464 (78.02%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSF-FCIWKLILQRRDQ-CCYLLGYECYKATEDRKLGVEACVNIILR 60
           M +F +  LL L   F F I+K + +RR+Q  CY+L YECYK  E+RKL  E C  ++ R
Sbjct: 1   MELFSLSSLLLLSTLFVFYIFKFVFKRRNQRNCYMLHYECYKGMEERKLDTETCAKVVQR 60

Query: 61  NRNLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLF 120
           N+NLGLEEYR+LL++M +SG+G ETYGP+N++  RE+SP+L DA SEMD++    LDKLF
Sbjct: 61  NKNLGLEEYRFLLRTMASSGIGEETYGPRNVLEGREDSPTLLDAHSEMDEIMFDTLDKLF 120

Query: 121 AKT--GVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAID 180
            KT   +SPSDIDILVVNVSLF+P+PSLT+R+INRYKMRED+K++NLSG+GCSAS+++ID
Sbjct: 121 HKTKGSISPSDIDILVVNVSLFAPSPSLTSRVINRYKMREDIKSYNLSGLGCSASVISID 180

Query: 181 LVKHLFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSA 240
           +V+ +F+T +NA A+VVSTE++GP+WY GK++ MML+NCL+R+GG S+L TN A  K+ A
Sbjct: 181 IVQRMFETRENALALVVSTETMGPHWYCGKDRSMMLSNCLFRAGGSSVLLTNAARFKNQA 240

Query: 241 LLKLKCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRIL 300
           L+KL  ++R H+GS +EAY C IQ+ED  GH GF LTK++K  A +ALT NLQ LLPR+L
Sbjct: 241 LMKLVTVVRAHVGSDDEAYSCCIQMEDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVL 300

Query: 301 PLRELLRYAILTHPLYKTINGNIEAKK-VRLNLKTGVDHFGIPPTERAVIDGIGKSLGLS 360
           P++EL+RYAI+     +T      A   + LNLKTG+ HF I P  RA+I+G+GKSLGL+
Sbjct: 301 PVKELIRYAIVRALKRRTSAKREPASSGIGLNLKTGLQHFCIHPGGRAIIEGVGKSLGLT 360

Query: 361 DYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVM 420
           ++D+EPARMALHRFGNTS+GGLWYVLGYMEAK RLK+G+KILM+S GAG+E N+CVWEV+
Sbjct: 361 EFDIEPARMALHRFGNTSSGGLWYVLGYMEAKNRLKKGEKILMMSMGAGFESNNCVWEVL 420

Query: 421 SNLEDENVWKDCIHSYPTKYTV-NLLKEKCSWLNDERLSFVKFD 459
            +L+D+NVW+D +  YP    + N   EK  W+ND+ +SFV+ D
Sbjct: 421 KDLDDKNVWEDSVDRYPELSRIPNPFVEKYDWINDDTMSFVRVD 464

BLAST of CmaCh04G002430 vs. TAIR 10
Match: AT2G28630.1 (3-ketoacyl-CoA synthase 12 )

HSP 1 Score: 486.1 bits (1250), Expect = 3.2e-137
Identity = 240/475 (50.53%), Postives = 323/475 (68.00%), Query Frame = 0

Query: 1   MGIFMVVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNR 60
           M +  +   L L Y FF IWKLI  ++D+ CY+L Y+C+K T+DR +  +    II RN+
Sbjct: 1   MDLLFLFFSLLLSYLFFKIWKLIDSKQDKDCYILDYQCHKPTDDRMVSTQFSGEIIYRNQ 60

Query: 61  NLGLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAK 120
           NLGL EY++LLK++V+SG+G +TY P+ +   REE PSL D ISEM++ +  ++ KL  +
Sbjct: 61  NLGLTEYKFLLKAIVSSGIGEQTYAPRLVFEGREERPSLQDGISEMEEFYVDSIGKLLER 120

Query: 121 TGVSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKH 180
             +SP DIDILVVNVS+ S  PSL +RIIN YKMR+DVK FNL+GMGCSAS++++D+VK+
Sbjct: 121 NQISPKDIDILVVNVSMLSSTPSLASRIINHYKMRDDVKVFNLTGMGCSASLISVDIVKN 180

Query: 181 LFKTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKL 240
           +FK+  N  A+V ++ES+ PNWYSG  + M+L NCL+RSGGC++L TNK +L+  A+ KL
Sbjct: 181 IFKSYANKLALVATSESLSPNWYSGNNRSMILANCLFRSGGCAILLTNKRSLRKKAMFKL 240

Query: 241 KCILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRE 300
           KC++RTH G+  E+Y C IQ ED+ G  GF L K++   AT+A   NL+ + P+ILP+ E
Sbjct: 241 KCMVRTHHGAREESYNCCIQAEDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTE 300

Query: 301 LLRYAILTHPLYKTI-------NGNIEAK-----KVRLNLKTGVDHFGIPPTERAVIDGI 360
           L+R+ +    L K I        G+         K  +N KTG++HF I    +AVIDGI
Sbjct: 301 LIRFMLKL--LIKKIKIRQNPSKGSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGI 360

Query: 361 GKSLGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECN 420
           G SL L++YD+EPARM LHRFGNTS   LWYVL YMEAKKRLK GD++ MISFGAG++CN
Sbjct: 361 GHSLDLNEYDIEPARMTLHRFGNTSASSLWYVLAYMEAKKRLKRGDRVFMISFGAGFKCN 420

Query: 421 SCVWEVMSNL----EDENVWKDCIHSYPTKYTVNLLKEKCSWLNDERLSFVKFDD 460
           SCVWEV+ +L       NVW  CI  YP K  +N   EK  W+ DE     K  D
Sbjct: 421 SCVWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDEDPDTFKVPD 473

BLAST of CmaCh04G002430 vs. TAIR 10
Match: AT1G07720.1 (3-ketoacyl-CoA synthase 3 )

HSP 1 Score: 476.1 bits (1224), Expect = 3.3e-134
Identity = 233/463 (50.32%), Postives = 325/463 (70.19%), Query Frame = 0

Query: 5   MVVCLLSLFYSF--FCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNL 64
           ++V LLSL  S+  F IWK I  +RDQ CY+L Y+C+K ++DR +  +   +IILRN++L
Sbjct: 3   LLVMLLSLLVSYLIFKIWKRIDSKRDQNCYILDYQCHKPSDDRMVNTQFSGDIILRNKHL 62

Query: 65  GLEEYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTG 124
            L EY++LLK++V+SG+G +TY P+     RE+ P+L D +SEM++ +   ++K+  +  
Sbjct: 63  RLNEYKFLLKAIVSSGIGEQTYAPRLFFEGREQRPTLQDGLSEMEEFYIDTIEKVLKRNK 122

Query: 125 VSPSDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLF 184
           +SPS+IDILVVNVS+ +  PSL+ARIIN YKMRED+K FNL+ MGCSAS+++ID+VK++F
Sbjct: 123 ISPSEIDILVVNVSMLNSTPSLSARIINHYKMREDIKVFNLTAMGCSASVISIDIVKNIF 182

Query: 185 KTNKNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKC 244
           KT KN  A+VV++ES+ PNWYSG  + M+L NCL+RSGGC++L TNK +L   A+ KL+C
Sbjct: 183 KTYKNKLALVVTSESLSPNWYSGNNRSMILANCLFRSGGCAVLLTNKRSLSRRAMFKLRC 242

Query: 245 ILRTHLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRELL 304
           ++RTH G+ ++++   +Q ED+ GH G  L K +   AT+A   NL+ + P+ILP+ ELL
Sbjct: 243 LVRTHHGARDDSFNACVQKEDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTELL 302

Query: 305 RYAIL-------THPLYKTINGNIEAK----KVRLNLKTGVDHFGIPPTERAVIDGIGKS 364
           R+ +        + P   + N    A     K  +N KTG+DHF I    +AVID IG S
Sbjct: 303 RFMLCLLLKKLRSSPSKGSTNVTQAAPKAGVKAGINFKTGIDHFCIHTGGKAVIDAIGYS 362

Query: 365 LGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCV 424
           L L++YDLEPARM LHRFGNTS   LWYVLGYMEAKKRLK GD++ MISFGAG++CNSCV
Sbjct: 363 LDLNEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKRGDRVFMISFGAGFKCNSCV 422

Query: 425 WEVMSNLE----DENVWKDCIHSYPTKYTVNLLKEKCSWLNDE 451
           WEV+ +L       NVW  CI+ YP K  +N   EK  W+++E
Sbjct: 423 WEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEE 465

BLAST of CmaCh04G002430 vs. TAIR 10
Match: AT1G01120.1 (3-ketoacyl-CoA synthase 1 )

HSP 1 Score: 340.5 bits (872), Expect = 2.1e-93
Identity = 177/433 (40.88%), Postives = 266/433 (61.43%), Query Frame = 0

Query: 6   VVCLLSLFYSFFCIWKLILQRRDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNLGLE 65
           + CL  +F SF  +  L +  R +  YL+ + CYK  ++RK+ V++ + +   N +   +
Sbjct: 101 LTCL--VFLSF--VLTLYVANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDD 160

Query: 66  EYRYLLKSMVNSGLGGETYGPKNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTGVSP 125
             ++  +    +GLG ETY P+ I +   +  ++S+A +E + V   ALD LF KTG+ P
Sbjct: 161 TVQFQQRISNRAGLGDETYLPRGITSTPPKL-NMSEARAEAEAVMFGALDSLFEKTGIKP 220

Query: 126 SDIDILVVNVSLFSPAPSLTARIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLFKTN 185
           +++ IL+VN SLF+P PSL+A I+N YKMRED+K++NL GMGCSA +++IDL  +L K N
Sbjct: 221 AEVGILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKAN 280

Query: 186 KNAYAVVVSTESIGPNWYSGKEQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKCILR 245
            N+YAVVVSTE+I  NWY G ++ M+L NC++R GG ++L +N+   +  +   L  ++R
Sbjct: 281 PNSYAVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVR 340

Query: 246 THLGSSNEAYGCSIQVEDDHGHRGFRLTKHIKAVATQALTINLQTLLPRILPLRELLRYA 305
           TH GS ++ Y C  Q ED+ G  G  L + + +VA  AL  N+ TL P +LPL E L + 
Sbjct: 341 THKGSDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFL 400

Query: 306 ILTHPLYKTINGNIEAKKVRLNLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLEPARMA 365
           I    L K     ++ K    + K   +HF I    RAV+D + K+L L D+ +EP+RM 
Sbjct: 401 I---SLVKRKMFKLKVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMT 460

Query: 366 LHRFGNTSTGGLWYVLGYMEAKKRLKEGDKILMISFGAGYECNSCVWEVMSNLEDE---- 425
           LHRFGNTS+  LWY + Y EAK R+K GD++  I+FG+G++CNS VW+ +  +  E    
Sbjct: 461 LHRFGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTG 520

Query: 426 NVWKDCIHSYPTK 435
           N W   I  YP K
Sbjct: 521 NAWAGSIDQYPVK 525

BLAST of CmaCh04G002430 vs. TAIR 10
Match: AT2G46720.1 (3-ketoacyl-CoA synthase 13 )

HSP 1 Score: 323.2 bits (827), Expect = 3.5e-88
Identity = 164/415 (39.52%), Postives = 254/415 (61.20%), Query Frame = 0

Query: 27  RDQCCYLLGYECYKATEDRKLGVEACVNIILRNRNLGLEEYRYLLKSMVNSGLGGETYGP 86
           R +  YL+ + C++ T+  K+  E   N+    +    E  +++ + +  SGLG +TY P
Sbjct: 56  RSKPVYLVDFSCHQPTDSCKISSETFFNMAKGAQLYTDETIQFMTRILNRSGLGDDTYSP 115

Query: 87  KNIIAAREESPSLSDAISEMDDVFSSALDKLFAKTGVSPSDIDILVVNVSLFSPAPSLTA 146
           + ++ +   +PS+ +A  E + V   AL+ LF KTG+ P ++ I +VN SLF+P PSL++
Sbjct: 116 RCMLTS-PPTPSMYEARHESELVIFGALNSLFKKTGIEPREVGIFIVNCSLFNPNPSLSS 175

Query: 147 RIINRYKMREDVKAFNLSGMGCSASIVAIDLVKHLFKTNKNAYAVVVSTESIGPNWYSGK 206
            I+NRYK++ DVK +NLSGMGCSA  +++DL  +L K N N YAV+VSTE++  + Y G 
Sbjct: 176 MIVNRYKLKTDVKTYNLSGMGCSAGAISVDLATNLLKANPNTYAVIVSTENMTLSMYRGN 235

Query: 207 EQPMMLTNCLYRSGGCSMLFTNKAALKDSALLKLKCILRTHLGSSNEAYGCSIQVEDDHG 266
           ++ M++ NCL+R GG +++ +N++  +  +  +L  I+RTH GSS++ Y C+ Q ED  G
Sbjct: 236 DRSMLVPNCLFRVGGAAVMLSNRSQDRVRSKYELTHIVRTHKGSSDKHYTCAEQKEDSKG 295

Query: 267 HRGFRLTKHIKAVATQALTINLQTLLPRILPLRELLRYAILTHPLYKTINGNIEAKKVRL 326
             G  L+K +  VA  +L  NL  L P +LPL E LR+ +    L K+    ++      
Sbjct: 296 IVGVALSKELTVVAGDSLKTNLTALGPLVLPLSEKLRFILF---LVKSKLFRLKVSPYVP 355

Query: 327 NLKTGVDHFGIPPTERAVIDGIGKSLGLSDYDLEPARMALHRFGNTSTGGLWYVLGYMEA 386
           + K    HF I    RA++D + K LGLS++DLEP+RM LHRFGNTS+  LWY L Y+EA
Sbjct: 356 DFKLCFKHFCIHAGGRALLDAVEKGLGLSEFDLEPSRMTLHRFGNTSSSSLWYELAYVEA 415

Query: 387 KKRLKEGDKILMISFGAGYECNSCVWEVMSNLEDE-----NVWKDCIHSYPTKYT 437
           K R+K GD++  ++FG+G++CNS VW  +  +        N W D +H YP   T
Sbjct: 416 KCRVKRGDRVWQLAFGSGFKCNSIVWRALRTIPANESLVGNPWGDSVHKYPVHVT 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZ722.7e-15758.193-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1[more]
Q9SIB24.4e-13650.533-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1[more]
Q9LQP84.6e-13350.323-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3[more]
Q9MAM33.0e-9240.883-ketoacyl-CoA synthase 1 OS=Arabidopsis thaliana OX=3702 GN=KCS1 PE=1 SV=1[more]
Q9ZUZ05.0e-8739.523-ketoacyl-CoA synthase 13 OS=Arabidopsis thaliana OX=3702 GN=HIC PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JLQ76.8e-273100.003-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111488046 PE=3 SV=1[more]
A0A6J1FLF19.2e-27098.933-ketoacyl-CoA synthase OS=Cucurbita moschata OX=3662 GN=LOC111446876 PE=3 SV=1[more]
A0A0A0KNS79.9e-24890.193-ketoacyl-CoA synthase OS=Cucumis sativus OX=3659 GN=Csa_5G178710 PE=3 SV=1[more]
A0A6J1CD512.2e-24789.133-ketoacyl-CoA synthase OS=Momordica charantia OX=3673 GN=LOC111010111 PE=3 SV=1[more]
A0A1S3CF496.4e-24789.553-ketoacyl-CoA synthase OS=Cucumis melo OX=3656 GN=LOC103500178 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022991407.11.4e-272100.003-ketoacyl-CoA synthase 19-like [Cucurbita maxima][more]
XP_023554275.12.9e-27099.153-ketoacyl-CoA synthase 19-like [Cucurbita pepo subsp. pepo][more]
XP_022941551.11.9e-26998.933-ketoacyl-CoA synthase 19-like [Cucurbita moschata][more]
KAG7030748.12.8e-26898.513-ketoacyl-CoA synthase 19 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6600079.15.2e-26798.083-ketoacyl-CoA synthase 19, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G04530.11.9e-15858.193-ketoacyl-CoA synthase 19 [more]
AT2G28630.13.2e-13750.533-ketoacyl-CoA synthase 12 [more]
AT1G07720.13.3e-13450.323-ketoacyl-CoA synthase 3 [more]
AT1G01120.12.1e-9340.883-ketoacyl-CoA synthase 1 [more]
AT2G46720.13.5e-8839.523-ketoacyl-CoA synthase 13 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0137473-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminalPFAMPF08541ACP_syn_III_Ccoord: 332..413
e-value: 4.2E-10
score: 39.7
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 77..414
e-value: 8.1E-59
score: 201.5
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 19..229
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 243..414
IPR013601FAE1/Type III polyketide synthase-like proteinPFAMPF08392FAE1_CUT1_RppAcoord: 24..306
e-value: 9.5E-114
score: 379.6
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePIRSFPIRSF036417Very_long_ch_3-ktacl-CoA_syncoord: 2..446
e-value: 2.2E-170
score: 565.3
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePANTHERPTHR315613-KETOACYL-COA SYNTHASEcoord: 1..456
NoneNo IPR availablePANTHERPTHR31561:SF1253-KETOACYL-COA SYNTHASEcoord: 1..456
NoneNo IPR availableCDDcd00831CHS_likecoord: 23..412
e-value: 3.26533E-101
score: 305.303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G002430.1CmaCh04G002430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0102756 very-long-chain 3-ketoacyl-CoA synthase activity
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups