CmaCh04G001840 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G001840
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	cucumisin-like
Location	Cma_Chr04: 894719 .. 898945 (-)
RNA-Seq Expression	CmaCh04G001840
Synteny	CmaCh04G001840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCCCAGAAAGTTCAGTGGTTGGAGATATGACTTTCTTCAAGCTTTGTATATAAAGGCCCTTCCTAAGCTGATCATATTTATGTTTCCAGTCATGACCATGGCAGGAAATAATGCGTCTTCTTCTTTGATCTTCAAGCTCGTCATTCTAAACCTCGTTTGTTGTCTGCTCGCTTCTAGCTTCGATTCTGGTAACGATGGGCGAAAGGTATAAAACATTCTACTTATAAATGTATATTTGGATTGTTTGGTATAAAAATTTTGTTGGATAAAACCTTGCAGGTTTATATTGTGTACTTGGGAAACAAGCGGGAGGATACGGCTTCAACTCCTTCACATCATATGAGAATGTTGGAAGAAACGATTGGCAGGTTCATCATCTTCATTTTTTTTTTTTTCTTTTGTTCTTATCAACAAGAAAGTTTCATGCATTAATTTATGTGATTTTCGTGTTCTTAGCACGTTCGCTCCCGAAGCTCTCCTCCATAGCTACAAGAGAAGTTTCAATGGATTCGTGGTTAGGCTCACCGAAGAAGAAGCTCAAAAGATTTCTGGTATCTATAACTATTCCAACGTCTTTAAATTCTTAGCCTTCAAAACTTTAACAACTCAAACCCACCGCTAACAGATATTTTTAACAACTCAAGTTTCTCCTCAAGGTTTTTAAAACGCGTTTACTAGGAAGAGGTTCTTTGAGGCCAGTATCTTTTCTGGGCACACCGCCTTGTGTCCACTCCTTTTATGGCTCAGCCTCCTCGCTCGTACATCTCCCCGTATGATACCATTTGTAACAACCCAAGCCCATCACTTACAAATATTGTCCTCTTTAGACTTTTTCTCAATGTTTTTAAAAGACATCTATCCGGGAGAGATTTTCATAACCTTGTAAATGATGTTTTGTTCTCCTTCCCAACATATGTGGGATTTCACACAAACTGAATGTATAACTTGGCATATTTCAGCTAAGGACGGTGTTGTCTCTGTGTTTCCAAACGAAAAGAAACATCCTCATACGACAAGATCATGGGATTTTATGGGTTTTACTACAAATGTTCCTCGTGTAAAACAAGTTGAAAGCGACATAGTAGTTGGAGTTTTGGACACAGGAATTTGGCCGGAGTCTCCTAGCTTTAGTGACGTAGGCTATGGCCCTCCACCAGCTAAATGGAAGGGCACTTGCCAAGCCTCTACCAACTTTCGTTGCAACAAGTAAACACTAATAAAATTTTGTCGCTAAATATATAGATTTAGTAATTTACCGTTAAAAATGTTTGAACTAATTGTCAGAAAAATTATCGGAGCTCGAGCATACCGTAGTGACAACAATTTTCCTCCCGAAGACTTTAAAAGTCCAAGAGATTCAGAAGGCCACGGGACACACACGGCATCGACTGTGGCCGGTGGTCTCGTGAGCCAGGCAAGTTTATTCGGTCTTGCGCTTGGCACAGCTAGAGGAGGGGTTCCTTCCGCGCGCATTGCTGTGTACAAGGTATGTTGGTCGGATGGGTGCGAGGATGCCGATATTCTTGCTGCATTCGACGATGCAATTGCCGATGGTGTAGATATCATATCTCTTTCAGTTGGGGGGAACGAACCCAAGTTTTACTTCAACGATTCAATTGCCATTGGAGCTTTCCATTCCATGAAGAATGGAATATTGACCTCCAACTCCGCTGGAAATGATGGCCCTGACTTCCTCACCGTCAGAAATTTCTCTCCGTGGTCTCTTTCCGTGGCTGCAAGCTCCATCGACAGAAAGTTGGTATCAAAAGTGCAGCTTGGCAACAATAATATCTTTCAGGTCATACTTTTTATCTCATGTTCCTATCTCTCTTTGATTTCTGGATAATTAACAACAAATTTTGGGTGTAGGGATTTACAATTAACACGTTTGATCTTCAACAAAAACAATTTCCTCTAATTTATGCTGGGAATGCACCTAATATCTCTGGAGGCTTCACTGGTGCTACCTCCCGGTATGATGTTGTCCATTCTGAGTATAAGCTCTCATGACTCTGCTTTTGACTTTTCTCAAAATATCTCATACTAATACCGATAATGTTTCTTATTTATAAACTTGTGATGTTCCTCTTAATTAGCCAATATGGACTACTCCTCTTAATAATCATCAAGAATTCTCTCTTGGAACAAAGTACACCCCTCCCTGAGGTCTATGGAGCCCTCGAACAAAGTACACTATTTGTTCAACACTTTAGTCACTTTTGACTACACTTTCGAGGGTTTCGATTTCTTTGTTTCACATTTGAGGATTCTATTGACTTGACTAAGTTTAGAGCATGACTCTGATAACATGTTAGGAACTACAACTCTCTACAATAGTATGATATTGTCCATTTTAAGCATAAACTTTCATGACTTTTCTTTTGATTTTTCCAAAAAGCCTCGTGTCAATGGAGATAGTGTTCTTATTTATAAACCCGTGATCTTCCTCCTAATGATCCTCAAAAGTTGTAGGGTTTCTTTTATCAATATTTCACACATGATTTAAAACAGATTTTGTTCGAGGAACTCAGTCGATCGCAACTTGGTGAAAGGAAAAATCCTTGTTTGTGATTCCATATTGTCTCCTTCAACATTTGCGTCCTTCAGTAGCGCAGTTGGCGTTGTTATGAATGATGCTGGCGTGAAAGATAATGCAAGGTCCTATCCCTTGCCTTCTTCCTACCTCGGCACAGTGGCTGGTAACAACATCAAAACCTACATGGGATCAAATAAGTACGTTTCGACATCCATATTAAATAGTTTTACTAGCACTATATGTTCGGATATTGACTCGGCTTGATTTGTGAATCGATAGCTTTCCGACTGCAACGATTTTCAAGAGCAATGCAGTGAACGATACATCTGCTCCTATAGTAGTTTCCTTCTCCTCGAGAGGACCCAATCCTGTAACCTTGGACATTCTCAAGGTTACATTCCTCTGCTTGTTTTCAATTGGTTTGTTCAATTCTGTAACATTTTGCTAAGATCATTTGTCTTCATCAACTTGCATGCATGCAGCCTGATTTGACTGCTCCTGGAGTTGAAATTCTCGCCGCATGGCCTCCTGATGCACCGGTCTCCGGTGGAATCAGAGATTCGAGGTCGACGCTCTATAATATAATCTCGGGGACGTCGATGTCTTGCCCACATGCCACCGCAGCTGCAGTGTACGTAAAAACATTCCATCCCACCTGGTCTCCCGCTGCCATTAAGTCAGCTCTCATGACAACGGGTAATGTTTCGTTTGTGTTAAGGATATTTAGTAATAAATTATAGTTTACCATATATATTATATTTGATATTTTATTATAAGGCAAATATAGATATTTGCCTTATTATCATAGGTATCTTTCTATTTATTGTTATTTTCATTTATTACTATTTCCATTTATTACTATTTCTATATCCTTGTAATTTATTTAATTATAAATAAGATAACTTTCACATCATTTAGGTGTGGTGGATTAATCAAACATTCACAGTTTGATAACCACTTGATTTTGCAAAAAATAAACCAAACAAAATTAAGTGAATTCACTTTTACAGCTATTCCTTTGAATGTGGGACTCAATCCACAAGCAGAGTTCGCATATGGTGCTGGCCATATCGACCCATTAAAGGCAACAAATCCAGGGTTGGTCTACGATGCCAATGAGACCGACTACGTGAACTTCTTGTGTGGCCAAGGTTACTCCACCGCCATGGTACGGCGTCTCACCGGCGACGGCAGTGTTTGTACCGCTGCCAACTCCGGCAGAGTTTGGGATCTAAACTATCCCTCCTTTGCACTCTCCACCACTCCTTCAGAATCCATCAACCAATTCTTCACAAGAACACTCACAAACGTGGACACGGAAGCCTCCACATATACATCTAAGATCCTTGGCGCCCCAGAAGGGCTCACAATCACAGTGGACCCGCCGGTTCTGTCATTCAATGGCATTGGAGACAAGAAATCTTTCACGTTAACCATTGATGGAACCATCATCAAAACCATAGTGTCTGCTTCTGTGGTGTGGAGCGATGGCTCTCACAATGTGAGAAGCCCTATTACAATATATATTGTCAACAAAGCTTAATTTGGTTTGTGTCTTTATTTAAAGTTGAAACTGTGAGCCACCCTTTACCCTTTTCTCGTTTTAAGGCGTCTCGCCATATCTCTAGTCCAGACTTGTGCACTATACTAATAATAATCTCCGATCGGTGATCTTTTCAGCTCTAA

mRNA sequence

ATGCTTCCCAGAAAGTTCAGTGGTTGGAGATATGACTTTCTTCAAGCTTTGTATATAAAGGCCCTTCCTAAGCTGATCATATTTATGTTTCCAGTCATGACCATGGCAGGAAATAATGCGTCTTCTTCTTTGATCTTCAAGCTCGTCATTCTAAACCTCGTTTGTTGTCTGCTCGCTTCTAGCTTCGATTCTGGTAACGATGGGCGAAAGGTTTATATTGTGTACTTGGGAAACAAGCGGGAGGATACGGCTTCAACTCCTTCACATCATATGAGAATGTTGGAAGAAACGATTGGCAGCACGTTCGCTCCCGAAGCTCTCCTCCATAGCTACAAGAGAAGTTTCAATGGATTCGTGGTTAGGCTCACCGAAGAAGAAGCTCAAAAGATTTCTGCTAAGGACGGTGTTGTCTCTGTGTTTCCAAACGAAAAGAAACATCCTCATACGACAAGATCATGGGATTTTATGGGTTTTACTACAAATGTTCCTCGTGTAAAACAAGTTGAAAGCGACATAGTAGTTGGAGTTTTGGACACAGGAATTTGGCCGGAGTCTCCTAGCTTTAGTGACGTAGGCTATGGCCCTCCACCAGCTAAATGGAAGGGCACTTGCCAAGCCTCTACCAACTTTCGTTGCAACAAAAAAATTATCGGAGCTCGAGCATACCGTAGTGACAACAATTTTCCTCCCGAAGACTTTAAAAGTCCAAGAGATTCAGAAGGCCACGGGACACACACGGCATCGACTGTGGCCGGTGGTCTCGTGAGCCAGGCAAGTTTATTCGGTCTTGCGCTTGGCACAGCTAGAGGAGGGGTTCCTTCCGCGCGCATTGCTGTGTACAAGGTATGTTGGTCGGATGGGTGCGAGGATGCCGATATTCTTGCTGCATTCGACGATGCAATTGCCGATGGTGTAGATATCATATCTCTTTCAGTTGGGGGGAACGAACCCAAGTTTTACTTCAACGATTCAATTGCCATTGGAGCTTTCCATTCCATGAAGAATGGAATATTGACCTCCAACTCCGCTGGAAATGATGGCCCTGACTTCCTCACCGTCAGAAATTTCTCTCCGTGGTCTCTTTCCGTGGCTGCAAGCTCCATCGACAGAAAGTTGGTATCAAAAGTGCAGCTTGGCAACAATAATATCTTTCAGGGATTTACAATTAACACGTTTGATCTTCAACAAAAACAATTTCCTCTAATTTATGCTGGGAATGCACCTAATATCTCTGGAGGCTTCACTGGTGCTACCTCCCGATTTTGTTCGAGGAACTCAGTCGATCGCAACTTGGTGAAAGGAAAAATCCTTGTTTGTGATTCCATATTGTCTCCTTCAACATTTGCGTCCTTCAGTAGCGCAGTTGGCGTTGTTATGAATGATGCTGGCGTGAAAGATAATGCAAGGTCCTATCCCTTGCCTTCTTCCTACCTCGGCACAGTGGCTGGTAACAACATCAAAACCTACATGGGATCAAATAACTTTCCGACTGCAACGATTTTCAAGAGCAATGCAGTGAACGATACATCTGCTCCTATAGTAGTTTCCTTCTCCTCGAGAGGACCCAATCCTGTAACCTTGGACATTCTCAAGCCTGATTTGACTGCTCCTGGAGTTGAAATTCTCGCCGCATGGCCTCCTGATGCACCGGTCTCCGGTGGAATCAGAGATTCGAGGTCGACGCTCTATAATATAATCTCGGGGACGTCGATGTCTTGCCCACATGCCACCGCAGCTGCAGTGTACGTAAAAACATTCCATCCCACCTGGTCTCCCGCTGCCATTAAGTCAGCTCTCATGACAACGGCTATTCCTTTGAATGTGGGACTCAATCCACAAGCAGAGTTCGCATATGGTGCTGGCCATATCGACCCATTAAAGGCAACAAATCCAGGGTTGGTCTACGATGCCAATGAGACCGACTACGTGAACTTCTTGTGTGGCCAAGGTTACTCCACCGCCATGGTACGGCGTCTCACCGGCGACGGCAGTGTTTGTACCGCTGCCAACTCCGGCAGAGTTTGGGATCTAAACTATCCCTCCTTTGCACTCTCCACCACTCCTTCAGAATCCATCAACCAATTCTTCACAAGAACACTCACAAACGTGGACACGGAAGCCTCCACATATACATCTAAGATCCTTGGCGCCCCAGAAGGGCTCACAATCACAGTGGACCCGCCGGTTCTGTCATTCAATGGCATTGGAGACAAGAAATCTTTCACGTTAACCATTGATGGAACCATCATCAAAACCATAGTGTCTGCTTCTGTGGTGTGGAGCGATGGCTCTCACAATGTGAGAAGCCCTATTACAATATATATTGTCAACAAAGCTTAATTTGGTTTGTGTCTTTATTTAAAGTTGAAACTGTGAGCCACCCTTTACCCTTTTCTCGTTTTAAGGCGTCTCGCCATATCTCTAGTCCAGACTTGTGCACTATACTAATAATAATCTCCGATCGGTGATCTTTTCAGCTCTAA

Coding sequence (CDS)

ATGCTTCCCAGAAAGTTCAGTGGTTGGAGATATGACTTTCTTCAAGCTTTGTATATAAAGGCCCTTCCTAAGCTGATCATATTTATGTTTCCAGTCATGACCATGGCAGGAAATAATGCGTCTTCTTCTTTGATCTTCAAGCTCGTCATTCTAAACCTCGTTTGTTGTCTGCTCGCTTCTAGCTTCGATTCTGGTAACGATGGGCGAAAGGTTTATATTGTGTACTTGGGAAACAAGCGGGAGGATACGGCTTCAACTCCTTCACATCATATGAGAATGTTGGAAGAAACGATTGGCAGCACGTTCGCTCCCGAAGCTCTCCTCCATAGCTACAAGAGAAGTTTCAATGGATTCGTGGTTAGGCTCACCGAAGAAGAAGCTCAAAAGATTTCTGCTAAGGACGGTGTTGTCTCTGTGTTTCCAAACGAAAAGAAACATCCTCATACGACAAGATCATGGGATTTTATGGGTTTTACTACAAATGTTCCTCGTGTAAAACAAGTTGAAAGCGACATAGTAGTTGGAGTTTTGGACACAGGAATTTGGCCGGAGTCTCCTAGCTTTAGTGACGTAGGCTATGGCCCTCCACCAGCTAAATGGAAGGGCACTTGCCAAGCCTCTACCAACTTTCGTTGCAACAAAAAAATTATCGGAGCTCGAGCATACCGTAGTGACAACAATTTTCCTCCCGAAGACTTTAAAAGTCCAAGAGATTCAGAAGGCCACGGGACACACACGGCATCGACTGTGGCCGGTGGTCTCGTGAGCCAGGCAAGTTTATTCGGTCTTGCGCTTGGCACAGCTAGAGGAGGGGTTCCTTCCGCGCGCATTGCTGTGTACAAGGTATGTTGGTCGGATGGGTGCGAGGATGCCGATATTCTTGCTGCATTCGACGATGCAATTGCCGATGGTGTAGATATCATATCTCTTTCAGTTGGGGGGAACGAACCCAAGTTTTACTTCAACGATTCAATTGCCATTGGAGCTTTCCATTCCATGAAGAATGGAATATTGACCTCCAACTCCGCTGGAAATGATGGCCCTGACTTCCTCACCGTCAGAAATTTCTCTCCGTGGTCTCTTTCCGTGGCTGCAAGCTCCATCGACAGAAAGTTGGTATCAAAAGTGCAGCTTGGCAACAATAATATCTTTCAGGGATTTACAATTAACACGTTTGATCTTCAACAAAAACAATTTCCTCTAATTTATGCTGGGAATGCACCTAATATCTCTGGAGGCTTCACTGGTGCTACCTCCCGATTTTGTTCGAGGAACTCAGTCGATCGCAACTTGGTGAAAGGAAAAATCCTTGTTTGTGATTCCATATTGTCTCCTTCAACATTTGCGTCCTTCAGTAGCGCAGTTGGCGTTGTTATGAATGATGCTGGCGTGAAAGATAATGCAAGGTCCTATCCCTTGCCTTCTTCCTACCTCGGCACAGTGGCTGGTAACAACATCAAAACCTACATGGGATCAAATAACTTTCCGACTGCAACGATTTTCAAGAGCAATGCAGTGAACGATACATCTGCTCCTATAGTAGTTTCCTTCTCCTCGAGAGGACCCAATCCTGTAACCTTGGACATTCTCAAGCCTGATTTGACTGCTCCTGGAGTTGAAATTCTCGCCGCATGGCCTCCTGATGCACCGGTCTCCGGTGGAATCAGAGATTCGAGGTCGACGCTCTATAATATAATCTCGGGGACGTCGATGTCTTGCCCACATGCCACCGCAGCTGCAGTGTACGTAAAAACATTCCATCCCACCTGGTCTCCCGCTGCCATTAAGTCAGCTCTCATGACAACGGCTATTCCTTTGAATGTGGGACTCAATCCACAAGCAGAGTTCGCATATGGTGCTGGCCATATCGACCCATTAAAGGCAACAAATCCAGGGTTGGTCTACGATGCCAATGAGACCGACTACGTGAACTTCTTGTGTGGCCAAGGTTACTCCACCGCCATGGTACGGCGTCTCACCGGCGACGGCAGTGTTTGTACCGCTGCCAACTCCGGCAGAGTTTGGGATCTAAACTATCCCTCCTTTGCACTCTCCACCACTCCTTCAGAATCCATCAACCAATTCTTCACAAGAACACTCACAAACGTGGACACGGAAGCCTCCACATATACATCTAAGATCCTTGGCGCCCCAGAAGGGCTCACAATCACAGTGGACCCGCCGGTTCTGTCATTCAATGGCATTGGAGACAAGAAATCTTTCACGTTAACCATTGATGGAACCATCATCAAAACCATAGTGTCTGCTTCTGTGGTGTGGAGCGATGGCTCTCACAATGTGAGAAGCCCTATTACAATATATATTGTCAACAAAGCTTAA

Protein sequence

MLPRKFSGWRYDFLQALYIKALPKLIIFMFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASVVWSDGSHNVRSPITIYIVNKA

Homology

[Close]

BLAST of CmaCh04G001840 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 7.3e-269
Identity = 475/735 (64.63%), Postives = 567/735 (77.14%), Query Frame = 0


Query: 41  SSSLIFKLVILNLVCC-LLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIG 100
           SSSLIFKL   +L     LAS  DS +DG+ +YIVY+G K ED  S   HH  MLE+ +G
Sbjct: 2   SSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVG 61

Query: 101 STFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFT 160
           STFAPE++LH+YKRSFNGF V+LTEEEA+KI++ +GVVSVF NE    HTTRSWDF+GF 
Sbjct: 62  STFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP 121

Query: 161 TNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGA 220
             VPR  QVES+IVVGVLDTGIWPESPSF D G+ PPP KWKGTC+ S NFRCN+KIIGA
Sbjct: 122 LTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGA 181

Query: 221 RAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAV 280
           R+Y       P D   PRD+ GHGTHTAST AGGLVSQA+L+GL LGTARGGVP ARIA 
Sbjct: 182 RSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAA 241

Query: 281 YKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILT 340
           YKVCW+DGC D DILAA+DDAIADGVDIISLSVGG  P+ YF D+IAIG+FH+++ GILT
Sbjct: 242 YKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 301

Query: 341 SNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQF 400
           SNSAGN GP+F T  + SPW LSVAAS++DRK V++VQ+GN   FQG +INTFD Q   +
Sbjct: 302 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ--YY 361

Query: 401 PLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSP-STFASFSSAVGVV 460
           PL+   + PN   GF  +TSRFC+  SV+ NL+KGKI+VC++   P   F S   A GV+
Sbjct: 362 PLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVL 421

Query: 461 MNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFS 520
           M  +  +D A SYPLPSS L          Y+ S   P ATIFKS  + + SAP+VVSFS
Sbjct: 422 MT-SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFS 481

Query: 521 SRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATA 580
           SRGPN  T D++KPD++ PGVEILAAWP  APV GGIR  R+TL+NIISGTSMSCPH T 
Sbjct: 482 SRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGIR--RNTLFNIISGTSMSCPHITG 541

Query: 581 AAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDAN 640
            A YVKT++PTWSPAAIKSALMTTA P+N   NPQAEFAYG+GH++PLKA  PGLVYDAN
Sbjct: 542 IATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDAN 601

Query: 641 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTR 700
           E+DYV FLCGQGY+T  VRR+TGD S CT+ N+GRVWDLNYPSF LS +PS++ NQ+F R
Sbjct: 602 ESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNR 661

Query: 701 TLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASV 760
           TLT+V  +ASTY + ++ AP+GLTI+V+P VLSFNG+GD+KSFTLT+ G+I   +VSAS+
Sbjct: 662 TLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASL 721

Query: 761 VWSDGSHNVRSPITI 774
           VWSDG H VRSPITI
Sbjct: 722 VWSDGVHYVRSPITI 727

[Close]

BLAST of CmaCh04G001840 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 3.7e-188
Identity = 353/734 (48.09%), Postives = 473/734 (64.44%), Query Frame = 0


Query: 51  LNLVCCLLASSFD-SGNDGRK---VYIVYLGNKREDTASTPSHHMRMLEETIGSTFAPEA 110
           L L+C     + D S ND R+   VYIVY+G   E   S PSHH+ +L++ +G+  A   
Sbjct: 8   LYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHL 67

Query: 111 LLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVK 170
           L+ SYKRSFNGF   L++ E+QK+     VVSVFP++     TTRSWDF+GF     R  
Sbjct: 68  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 127

Query: 171 QVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDN 230
             ESD++VGV+D+GIWPES SF D G+GPPP KWKG+C+    F CN K+IGAR Y   N
Sbjct: 128 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY---N 187

Query: 231 NFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSD 290
            F      S RD EGHGTHTAST AG  V  AS +GLA GTARGGVPSARIA YKVC+ +
Sbjct: 188 KFA----DSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-N 247

Query: 291 GCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGND 350
            C D DILAAFDDAIADGVD+IS+S+  +      N S+AIG+FH+M  GI+T+ SAGN+
Sbjct: 248 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 307

Query: 351 GPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGN 410
           GPD  +V N SPW ++VAAS  DR+ + +V LGN     G ++NTF+L   +FP++Y   
Sbjct: 308 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY--- 367

Query: 411 APNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKD 470
             N+S   + A + +CS   VD  LVKGKI++CD  L     A  + A+GV++ +  + D
Sbjct: 368 GQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPD 427

Query: 471 NARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVT 530
           +A   P P+S LG     +IK+Y+ S   P A I ++  + D  AP V SFSSRGP+ V 
Sbjct: 428 SAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVI 487

Query: 531 LDILKPDLTAPGVEILAAWPPDAPVSGGI--RDSRSTLYNIISGTSMSCPHATAAAVYVK 590
            ++LKPD++APG+EILAA+ P A  S  +   D RS  Y+++SGTSM+CPH    A YVK
Sbjct: 488 QNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVK 547

Query: 591 TFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVN 650
           +FHP WSP+AIKSA+MTTA P+N+  NP+ EFAYG+G I+P KA++PGLVY+    DY+ 
Sbjct: 548 SFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLK 607

Query: 651 FLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVD 710
            LC +G+ +  +   +G    C+      V DLNYP+     +  +  N  F RT+TNV 
Sbjct: 608 MLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVG 667

Query: 711 TEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIK--TIVSASVVWSD 770
              STY + ++     L I+++P +L F  + +KKSF +TI G  +K  + VS+SVVWSD
Sbjct: 668 FPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSD 727

Query: 771 GSHNVRSPITIYIV 777
           GSH+VRSPI  Y +
Sbjct: 728 GSHSVRSPIVAYSI 727

[Close]

BLAST of CmaCh04G001840 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 1.7e-180
Identity = 348/750 (46.40%), Postives = 480/750 (64.00%), Query Frame = 0


Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTP-SHHMRM 94
           MA   ASSSL+  L++L L      SS  +  D ++VYIVY+G+       TP S HM +
Sbjct: 1   MATLAASSSLLSCLLVLFL------SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNI 60

Query: 95  LEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSW 154
           L+E  G +     L+ SYKRSFNGF  RLTE E ++++   GVVSVFPN+K    TT SW
Sbjct: 61  LQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSW 120

Query: 155 DFMGFTTNV--PRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFR 214
           DFMG    +   R   VESD ++GV+D+GI PES SFSD G+GPPP KWKG C    NF 
Sbjct: 121 DFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT 180

Query: 215 CNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGG 274
           CN K+IGAR Y S+           RD +GHGTHTAST AG  V  AS FG+  GT RGG
Sbjct: 181 CNNKLIGARDYTSEGT---------RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGG 240

Query: 275 VPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFH 334
           VP++R+A YKVC   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH
Sbjct: 241 VPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFH 300

Query: 335 SMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINT 394
           +M  G+LT NSAGN GP  ++V   +PW L+VAAS+ +R  V+KV LGN     G ++N 
Sbjct: 301 AMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNA 360

Query: 395 FDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASF 454
           ++++ K +PL+Y  +A   S      ++  C  + VD++ VKGKILVC          S 
Sbjct: 361 YEMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV 420

Query: 455 SSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSA 514
             AVG++       D A  +PLP++ L T    ++ +Y+ S + P A + K+ A+ + ++
Sbjct: 421 -GAVGLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTS 480

Query: 515 PIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSM 574
           P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P    S    D+R   Y+++SGTSM
Sbjct: 481 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSM 540

Query: 575 SCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN---VGLNPQAEFAYGAGHIDPLKA 634
           SCPH    A YVKTF+P WSP+ I+SA+MTTA P+N    G+    EFAYG+GH+DP+ A
Sbjct: 541 SCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGI-ASTEFAYGSGHVDPIAA 600

Query: 635 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 694
           +NPGLVY+ +++D++ FLCG  Y++ +++ ++G+   C+ A      +LNYPS +   + 
Sbjct: 601 SNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSG 660

Query: 695 S-ESINQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLTID 754
           S  +    F RTLTNV T  STYTSK++ G    L + + P VLSF  + +K+SFT+T+ 
Sbjct: 661 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 720

Query: 755 GTIIKTIV--SASVVWSDGSHNVRSPITIY 775
           G+ + + V  SA+++WSDG+HNVRSPI +Y
Sbjct: 721 GSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

[Close]

BLAST of CmaCh04G001840 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 1.3e-177
Identity = 336/735 (45.71%), Postives = 472/735 (64.22%), Query Frame = 0


Query: 53  LVCCLLASSFDSGNDG-RKVYIVYLGNKREDTASTP-SHHMRMLEETIGSTFAPEALLHS 112
           LV  L + S D  + G ++VYIVYLG+       TP S HM +L+E  G +     L+ S
Sbjct: 15  LVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRS 74

Query: 113 YKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNV--PRVKQV 172
           YK+SFNGF  RLTE E ++++  + VVSVFP+ K    TT SW+FMG    +   R + +
Sbjct: 75  YKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSI 134

Query: 173 ESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNF 232
           ESD ++GV+D+GI+PES SFSD G+GPPP KWKGTC    NF CN K+IGAR Y + +  
Sbjct: 135 ESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS-- 194

Query: 233 PPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGC 292
             +  ++ RD  GHGTHTAS  AG  V+ ++ +GL  GTARGGVP+ARIAVYKVC ++GC
Sbjct: 195 --KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGC 254

Query: 293 EDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGP 352
           +   +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP
Sbjct: 255 DGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGP 314

Query: 353 DFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAP 412
              TV + +PW  SVAAS  +R  ++KV LG+  I  G ++NT+D+    +PL+Y  +A 
Sbjct: 315 KISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAA 374

Query: 413 NISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNA 472
             +     A  R C    +D  LVKGKI++CDS           +   +V N    +   
Sbjct: 375 LSTCSVDKA--RLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFI 434

Query: 473 RSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLD 532
           RS+P+  S+L      ++ +YM S   P AT+ KS  +++  AP+V SFSSRGP+ +  D
Sbjct: 435 RSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSD 494

Query: 533 ILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHP 592
           ILKPD+TAPGVEILAA+ PD+  +    D+R   Y+++SGTSM+CPH    A YVKTFHP
Sbjct: 495 ILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHP 554

Query: 593 TWSPAAIKSALMTTAIPLNVGLN--PQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFL 652
            WSP+ I+SA+MTTA P+N   +     EFAYG+GH+DP+ A NPGLVY+  + D++NFL
Sbjct: 555 QWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFL 614

Query: 653 CGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESINQFFTRTLTNVDT 712
           CG  Y++  +R ++GD S CT   S  +  +LNYP+ +   + ++  N  F RT+TNV  
Sbjct: 615 CGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGM 674

Query: 713 EASTYTSKILGAP-EGLTITVDPPVLSFNGIGDKKSFTLTIDGTII--KTIVSASVVWSD 772
           + STY +K++  P   L+I V P VLS   + +K+SF +T+    I  K  VSA+++WSD
Sbjct: 675 QKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSD 734

Query: 773 GSHNVRSPITIYIVN 778
           G+HNVRSPI +Y ++
Sbjct: 735 GTHNVRSPIIVYAMS 741

[Close]

BLAST of CmaCh04G001840 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 3.9e-177
Identity = 345/754 (45.76%), Postives = 478/754 (63.40%), Query Frame = 0


Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLG--NKREDTASTPSHHMR 94
           MA   AS+ L   L++L     LL+S     ++  +VYIVY+G  + R D   T S HM 
Sbjct: 1   MANLAASTCLYSWLLVL-----LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPT-SDHMS 60

Query: 95  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 154
           +L++  G +     L+ SYKRSFNGF  RLTE E   I+  +GVVSVFPN+    HTT S
Sbjct: 61  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120

Query: 155 WDFMGFT--TNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNF 214
           WDFMG     N  R   +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C    NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180

Query: 215 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 274
            CN K+IGAR Y S+           RD+ GHGTHTAST AG  V   S FG+  GT RG
Sbjct: 181 TCNNKLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 240

Query: 275 GVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 334
           GVP++RIA YKVC   GC    +L++FDDAIADGVD+I++S+G   P  + +D IAIGAF
Sbjct: 241 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 300

Query: 335 HSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 394
           H+M  GILT +SAGN GP   TV + +PW  +VAAS+ +R  ++KV LGN     G ++N
Sbjct: 301 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 360

Query: 395 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFAS 454
            FD++ K++PL+Y  +A   S      T+  C+   ++++ VKGKILVC     PS +  
Sbjct: 361 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 420

Query: 455 FSSAVGVVMNDAGVK-DNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDT 514
             S   + + D   + D A ++ LP+S L      ++ +Y+ S + P A + K+  + + 
Sbjct: 421 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 480

Query: 515 SAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGT 574
           ++P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P+   S    D+R   Y++ SGT
Sbjct: 481 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 540

Query: 575 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV---GLNPQAEFAYGAGHIDPL 634
           SM+CPH    A YVKTF+P WSP+ I+SA+MTTA P+     G+    EFAYGAGH+DP+
Sbjct: 541 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGI-ASTEFAYGAGHVDPM 600

Query: 635 KATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALST 694
            A NPGLVY+ ++ D++ FLCG  Y++  ++ ++GD   C+  N     +LNYPS +   
Sbjct: 601 AALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKL 660

Query: 695 TPSES-INQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLT 754
           + ++S  +  F RTLTNV T  STY SK++ G    L+I V P VL F  + +K+SF++T
Sbjct: 661 SGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVT 720

Query: 755 IDGTIIKTIV--SASVVWSDGSHNVRSPITIYIV 777
           + G+ + + V  SA+++WSDG+HNVRSPI +YI+
Sbjct: 721 VTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731

[Close]

BLAST of CmaCh04G001840 vs. ExPASy TrEMBL
Match: A0A6J1FQ02 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447181 PE=3 SV=1)

HSP 1 Score: 1532.3 bits (3966), Expect = 0.0e+00
Identity = 763/779 (97.95%), Postives = 771/779 (98.97%), Query Frame = 0


Query: 1   MLPRKFSGWRYDFLQALYIKALPKLIIFMFPVMTMAGNNASSSLIFKLVILNLVCCLLAS 60
           +LPRKFSGWRYDFLQALYIKALPK IIFMFPVM MAGNNASSSLIFKLV+LNLVCCLLAS
Sbjct: 7   LLPRKFSGWRYDFLQALYIKALPKRIIFMFPVMIMAGNNASSSLIFKLVVLNLVCCLLAS 66

Query: 61  SFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVV 120
           SFDSGNDGRKVYIVYLGNKREDTASTPSHH RMLEETIGSTFAPEALLHSYKRSFNGFVV
Sbjct: 67  SFDSGNDGRKVYIVYLGNKREDTASTPSHHTRMLEETIGSTFAPEALLHSYKRSFNGFVV 126

Query: 121 RLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTG 180
           RLTEEEAQKISAK+GVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTG
Sbjct: 127 RLTEEEAQKISAKEGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTG 186

Query: 181 IWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSE 240
           IWPESPSF+DVGYGPPPAKWKG CQ STNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSE
Sbjct: 187 IWPESPSFNDVGYGPPPAKWKGACQTSTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSE 246

Query: 241 GHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDA 300
           GHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYK+CWSDGCEDADILAAFDDA
Sbjct: 247 GHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKICWSDGCEDADILAAFDDA 306

Query: 301 IADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWS 360
           IADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLT+RNFSPWS
Sbjct: 307 IADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTIRNFSPWS 366

Query: 361 LSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSR 420
           LSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSR
Sbjct: 367 LSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSR 426

Query: 421 FCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGT 480
           FCSRNSVDRNLVKGKILVCDSI+SPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGT
Sbjct: 427 FCSRNSVDRNLVKGKILVCDSIVSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGT 486

Query: 481 VAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVE 540
           VAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNP TLDILKPDLTAPGVE
Sbjct: 487 VAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPETLDILKPDLTAPGVE 546

Query: 541 ILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALM 600
           ILAAWPPDAPVS GIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALM
Sbjct: 547 ILAAWPPDAPVSSGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALM 606

Query: 601 TTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLT 660
           TTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLT
Sbjct: 607 TTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLT 666

Query: 661 GDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEG 720
           GDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEG
Sbjct: 667 GDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEG 726

Query: 721 LTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           LTITVDPP LSFNGIGDKKSFTLTIDGTII+TIVSASVVWSDGSHNVRSPITIYIVNKA
Sbjct: 727 LTITVDPPALSFNGIGDKKSFTLTIDGTIIQTIVSASVVWSDGSHNVRSPITIYIVNKA 785

[Close]

BLAST of CmaCh04G001840 vs. ExPASy TrEMBL
Match: A0A6J1K168 (cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 712/751 (94.81%), Postives = 732/751 (97.47%), Query Frame = 0


Query: 29  MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS 88
           MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS
Sbjct: 1   MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS 60

Query: 89  HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH 148
           HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH
Sbjct: 61  HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH 120

Query: 149 TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST 208
           TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST
Sbjct: 121 TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST 180

Query: 209 NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA 268
           NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA
Sbjct: 181 NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA 240

Query: 269 RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG 328
           RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG
Sbjct: 241 RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG 300

Query: 329 AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFT 388
           AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQG+T
Sbjct: 301 AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGYT 360

Query: 389 INTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTF 448
           INTFDLQ K +PLIYAG+APNISGGF+G++SR+CS+NSVDRNLVKGKILVCDSILSPSTF
Sbjct: 361 INTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTF 420

Query: 449 ASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVND 508
           AS SSAVGVVMN+AGVKDNARSYPLPSSYL   AGN++K Y+GSNNFPTATIFKSN VND
Sbjct: 421 ASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVND 480

Query: 509 TSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISG 568
           T+APIVVSFSSRGPNP T DILKPDLTAPGVEILAAW P APVS GI+DSR+TLYNIISG
Sbjct: 481 TAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISG 540

Query: 569 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKA 628
           TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV LNPQAEFAYGAGHIDPLKA
Sbjct: 541 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKA 600

Query: 629 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 688
            NPGL+YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP
Sbjct: 601 MNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 660

Query: 689 SESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGT 748
           SESINQFFTRTLTNV +EASTYTSKILGAPEGLTITVDPPVLSFNGIG K+SFTLTI GT
Sbjct: 661 SESINQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGT 720

Query: 749 IIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           I ++IVSASVVWSDGSHNVRSPITIYIVNKA
Sbjct: 721 ISQSIVSASVVWSDGSHNVRSPITIYIVNKA 751

[Close]

BLAST of CmaCh04G001840 vs. ExPASy TrEMBL
Match: A0A6J1FVT5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447322 PE=3 SV=1)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 663/738 (89.84%), Postives = 698/738 (94.58%), Query Frame = 0


Query: 42  SSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIGST 101
           S +IFKLV L    CLLASS DS NDGRKVYIVYLGNK +D ASTPSHHMR+LEE +GST
Sbjct: 17  SYVIFKLVFLGFFGCLLASSLDSVNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGST 76

Query: 102 FAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTN 161
           FAP+ALLHSYKRSFNGFVVRLTE EAQKI AKDGVVSVFPN +KH HTTRSWDFMGFTTN
Sbjct: 77  FAPDALLHSYKRSFNGFVVRLTEGEAQKIRAKDGVVSVFPNGRKHLHTTRSWDFMGFTTN 136

Query: 162 VPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARA 221
           VPRV QVESDIVVGVLD+GIWPESPSFSDVGYGPPPAKWKG CQASTNFRCNKKIIGARA
Sbjct: 137 VPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQASTNFRCNKKIIGARA 196

Query: 222 YRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYK 281
           YRSDNNFPPEDF+SPRDS+GHGTHTASTVAGGLVSQASL+GLALGTARGGVPSARIAVYK
Sbjct: 197 YRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYK 256

Query: 282 VCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSN 341
           +CWSDGC DADILAAFDDAIADGVDIISLSVGG+EP++YFNDSIAIGAFHSMK+GILTSN
Sbjct: 257 ICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSN 316

Query: 342 SAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPL 401
           SAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQG+TINTFDLQ KQ+PL
Sbjct: 317 SAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGYTINTFDLQGKQYPL 376

Query: 402 IYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMND 461
           IYAG+APNISGGFT ++SR+CS+NSVDRNLVKGKI+VCDSILSPSTF S SSAVGVVMND
Sbjct: 377 IYAGHAPNISGGFTSSSSRYCSQNSVDRNLVKGKIVVCDSILSPSTFVSLSSAVGVVMND 436

Query: 462 AGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRG 521
           AG+KDNARSYPLPSSYL   AGN++K YMGSNNFPTATI KSN VNDTSAPIVVSFSSRG
Sbjct: 437 AGLKDNARSYPLPSSYLTRAAGNHVKNYMGSNNFPTATIHKSNEVNDTSAPIVVSFSSRG 496

Query: 522 PNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAV 581
           PNP T DILKPDLTAPGVEILAAW P APVS GI+DSR+TLYNIISGTSMSCPHATAAAV
Sbjct: 497 PNPETNDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAV 556

Query: 582 YVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETD 641
           YVKTFHPTWSPAAIKSALMTTAIPLNV LNPQAEFAYGAGHIDPLKATNPGLVYDANETD
Sbjct: 557 YVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKATNPGLVYDANETD 616

Query: 642 YVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLT 701
           YVNFLCGQGYSTAMV+RLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLT
Sbjct: 617 YVNFLCGQGYSTAMVQRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLT 676

Query: 702 NVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASVVWS 761
           NV +EASTYTSKILGAPEGLTITVDPP LSFN IGD+KSFTLTIDGTI ++IVSASV WS
Sbjct: 677 NVGSEASTYTSKILGAPEGLTITVDPPALSFNSIGDEKSFTLTIDGTISQSIVSASVEWS 736

Query: 762 DGSHNVRSPITIYIVNKA 780
           DGSHNVRSPITIYIVNKA
Sbjct: 737 DGSHNVRSPITIYIVNKA 754

[Close]

BLAST of CmaCh04G001840 vs. ExPASy TrEMBL
Match: A0A6J1K719 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 663/752 (88.16%), Postives = 704/752 (93.62%), Query Frame = 0


Query: 29  MFPVMT-MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTP 88
           MF +++ +  +   SS+IFKLV L    CLLASS DS NDGRKVYIVYLGNK +D ASTP
Sbjct: 1   MFDILSFLTRSIMGSSVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTP 60

Query: 89  SHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHP 148
           SHHMR+LEE +GSTF+P+ALLHSYKRSFNGF VRLTEEEAQKI AKDGVVSVFPN KKH 
Sbjct: 61  SHHMRLLEEVVGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHL 120

Query: 149 HTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAS 208
           HTTRSWDFMGFTTNVPRV QVESDIVVGVLD+GIWPESPSFSDVGYGP PAKWKG CQAS
Sbjct: 121 HTTRSWDFMGFTTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQAS 180

Query: 209 TNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGT 268
            NFRCNKKIIGARAYRSDNNFPPEDF+SPRDS+GHGTHTASTVAGGLVSQASL+GLALGT
Sbjct: 181 NNFRCNKKIIGARAYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGT 240

Query: 269 ARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAI 328
           ARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+EPK+YFNDSIAI
Sbjct: 241 ARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAI 300

Query: 329 GAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGF 388
           GAFHSMK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVS VQLGN NIFQG+
Sbjct: 301 GAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGY 360

Query: 389 TINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPST 448
           TINTFDLQ K +PLIYAG+APNISGGF+G++SR+CS+NSVDRNLVKGKILVCDSILSPST
Sbjct: 361 TINTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPST 420

Query: 449 FASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVN 508
           FAS SSAVGVVMN+AGVKDNARSYPLPSSYL   AGN++K Y+GSNNFPTATIFKSN VN
Sbjct: 421 FASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVN 480

Query: 509 DTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIIS 568
           DT+APIVVSFSSRGPNP T DILKPDLTAPGVEILAAW P APVS GI+DSR+TLYNIIS
Sbjct: 481 DTAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIIS 540

Query: 569 GTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLK 628
           GTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV LNPQAEFAYGAGHIDPLK
Sbjct: 541 GTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLK 600

Query: 629 ATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTT 688
           A NPGL+YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTT
Sbjct: 601 AMNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTT 660

Query: 689 PSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDG 748
           PSESINQFFTRTLTNV +EASTYTSKILGAPEGLTITVDPPVLSFNGIG K+SFTLTI G
Sbjct: 661 PSESINQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGG 720

Query: 749 TIIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           TI ++IVSASVVWSDGSHNVRSPITIYIVNKA
Sbjct: 721 TISQSIVSASVVWSDGSHNVRSPITIYIVNKA 752

[Close]

BLAST of CmaCh04G001840 vs. ExPASy TrEMBL
Match: A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)

HSP 1 Score: 1267.7 bits (3279), Expect = 0.0e+00
Identity = 625/741 (84.35%), Postives = 688/741 (92.85%), Query Frame = 0


Query: 41  SSSLIFKLVI-LNLVCCLLASSFDSG-NDGRKVYIVYLGNKREDTASTPSHHMRMLEETI 100
           SSSLIFKLVI ++    LLAS  DS  NDGRKVYIVYLGNK ED+ASTPSHHMRMLEE +
Sbjct: 2   SSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVV 61

Query: 101 GSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGF 160
           GSTFAP+ALLHSYKRSFNGFVV+LTEEEAQKISAK+GVVSVFPN KKH HTTRSWDFMGF
Sbjct: 62  GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGF 121

Query: 161 TTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIG 220
           T NV RVKQVES+IVVGVLD+GIWPESPSFSDVGYGPPPAKWKG CQ S NFRCN+KIIG
Sbjct: 122 TKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 181

Query: 221 ARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIA 280
           ARAYRSDN+FPPED KSPRDS+GHGTHTASTVAGGLV+QASL+GLALGTARGGVPSARIA
Sbjct: 182 ARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 241

Query: 281 VYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGIL 340
           VYK+CWSDGC DADILAAFDDAIADGVDIISLSVGGN+PK+YFNDSIAIGAFHSMK+GIL
Sbjct: 242 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 301

Query: 341 TSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQ 400
           TSNSAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVSKVQLGN N++QG+TINTFDL+ KQ
Sbjct: 302 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQ 361

Query: 401 FPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVV 460
           +PLIYAGNAPN+SGGFTG++SRFCSRNSVDRNLV+GKIL+CDSILSPSTFASF+ AVGVV
Sbjct: 362 YPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVV 421

Query: 461 MNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFS 520
           MNDAGVKDNARSYPLPSSYLG VAGNNIKTYMGS+ FPTATIFKSNAVNDTSAP++VSFS
Sbjct: 422 MNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSFS 481

Query: 521 SRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATA 580
           SRGPNP T DILKPDLTAPGVEILAAW P A VS G+ DSR+TLYNIISGTSMSCPHATA
Sbjct: 482 SRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATA 541

Query: 581 AAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDAN 640
           AAVYVKTFHP+WSPAAIKSALMTTAIPLN  LNPQAEFAYGAGHI+P+KA NPGLVYDA 
Sbjct: 542 AAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQ 601

Query: 641 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTR 700
           E+DYV FLCGQGY+TAMVRRL+GD SVCT ANSGRVWDLNYPSFALS+TPSESINQFF R
Sbjct: 602 ESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFRR 661

Query: 701 TLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASV 760
           T+TNV ++ +TY +K+LG P GLTI+V+PPVLSFN IG KKSFT+T+ G + + IVSA++
Sbjct: 662 TVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAAL 721

Query: 761 VWSDGSHNVRSPITIYIVNKA 780
           +W+DG H+VRSPIT+Y+V+KA
Sbjct: 722 LWTDGHHHVRSPITVYVVDKA 742

[Close]

BLAST of CmaCh04G001840 vs. NCBI nr
Match: XP_022941974.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1532.3 bits (3966), Expect = 0.0e+00
Identity = 763/779 (97.95%), Postives = 771/779 (98.97%), Query Frame = 0


Query: 1   MLPRKFSGWRYDFLQALYIKALPKLIIFMFPVMTMAGNNASSSLIFKLVILNLVCCLLAS 60
           +LPRKFSGWRYDFLQALYIKALPK IIFMFPVM MAGNNASSSLIFKLV+LNLVCCLLAS
Sbjct: 7   LLPRKFSGWRYDFLQALYIKALPKRIIFMFPVMIMAGNNASSSLIFKLVVLNLVCCLLAS 66

Query: 61  SFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVV 120
           SFDSGNDGRKVYIVYLGNKREDTASTPSHH RMLEETIGSTFAPEALLHSYKRSFNGFVV
Sbjct: 67  SFDSGNDGRKVYIVYLGNKREDTASTPSHHTRMLEETIGSTFAPEALLHSYKRSFNGFVV 126

Query: 121 RLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTG 180
           RLTEEEAQKISAK+GVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTG
Sbjct: 127 RLTEEEAQKISAKEGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTG 186

Query: 181 IWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSE 240
           IWPESPSF+DVGYGPPPAKWKG CQ STNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSE
Sbjct: 187 IWPESPSFNDVGYGPPPAKWKGACQTSTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSE 246

Query: 241 GHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDA 300
           GHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYK+CWSDGCEDADILAAFDDA
Sbjct: 247 GHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKICWSDGCEDADILAAFDDA 306

Query: 301 IADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWS 360
           IADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLT+RNFSPWS
Sbjct: 307 IADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTIRNFSPWS 366

Query: 361 LSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSR 420
           LSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSR
Sbjct: 367 LSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSR 426

Query: 421 FCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGT 480
           FCSRNSVDRNLVKGKILVCDSI+SPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGT
Sbjct: 427 FCSRNSVDRNLVKGKILVCDSIVSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGT 486

Query: 481 VAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVE 540
           VAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNP TLDILKPDLTAPGVE
Sbjct: 487 VAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPETLDILKPDLTAPGVE 546

Query: 541 ILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALM 600
           ILAAWPPDAPVS GIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALM
Sbjct: 547 ILAAWPPDAPVSSGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALM 606

Query: 601 TTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLT 660
           TTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLT
Sbjct: 607 TTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLT 666

Query: 661 GDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEG 720
           GDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEG
Sbjct: 667 GDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEG 726

Query: 721 LTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           LTITVDPP LSFNGIGDKKSFTLTIDGTII+TIVSASVVWSDGSHNVRSPITIYIVNKA
Sbjct: 727 LTITVDPPALSFNGIGDKKSFTLTIDGTIIQTIVSASVVWSDGSHNVRSPITIYIVNKA 785

[Close]

BLAST of CmaCh04G001840 vs. NCBI nr
Match: XP_023546659.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1459.5 bits (3777), Expect = 0.0e+00
Identity = 730/751 (97.20%), Postives = 740/751 (98.54%), Query Frame = 0


Query: 29  MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS 88
           MFPVM MAGNNASSSLIFKLV+LNLVCCLLASSF  GNDGRKVYIVY+GNKREDTASTPS
Sbjct: 1   MFPVMIMAGNNASSSLIFKLVVLNLVCCLLASSF--GNDGRKVYIVYMGNKREDTASTPS 60

Query: 89  HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH 148
           HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAK+GVVSVFPNEKK+PH
Sbjct: 61  HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKYPH 120

Query: 149 TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST 208
           TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKG CQAST
Sbjct: 121 TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGACQAST 180

Query: 209 NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA 268
           NFRCNKKIIGARAYRSDNNFPPEDFKSPRDS GHGTHTASTVAGGLVSQASLFGLALGTA
Sbjct: 181 NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSGGHGTHTASTVAGGLVSQASLFGLALGTA 240

Query: 269 RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG 328
           RGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG
Sbjct: 241 RGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG 300

Query: 329 AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFT 388
           AFHSMKNGILTSNSAGNDGPDFLT+RNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFT
Sbjct: 301 AFHSMKNGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFT 360

Query: 389 INTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTF 448
           INTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSI+SPSTF
Sbjct: 361 INTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIVSPSTF 420

Query: 449 ASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVND 508
           ASFSSAVGVVMNDAGVKDN RSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVND
Sbjct: 421 ASFSSAVGVVMNDAGVKDNVRSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVND 480

Query: 509 TSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISG 568
           TSAPIVVSFSSRGPNP TLDILKPDLTAPGVEILAAWPPDAPVS GIRDSRSTLYNIISG
Sbjct: 481 TSAPIVVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPDAPVSSGIRDSRSTLYNIISG 540

Query: 569 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKA 628
           TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA+PLNV LNPQAEFAYGAGHIDPLKA
Sbjct: 541 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAMPLNVELNPQAEFAYGAGHIDPLKA 600

Query: 629 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 688
           TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP
Sbjct: 601 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 660

Query: 689 SESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGT 748
           SESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPP LSFNGIGDKKSFTLTIDGT
Sbjct: 661 SESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGT 720

Query: 749 IIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           II++IVSASVVWSDGSHNVRSPITIYIVNKA
Sbjct: 721 IIQSIVSASVVWSDGSHNVRSPITIYIVNKA 749

[Close]

BLAST of CmaCh04G001840 vs. NCBI nr
Match: KAG6600027.1 (hypothetical protein SDJN03_05260, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1444.5 bits (3738), Expect = 0.0e+00
Identity = 722/746 (96.78%), Postives = 731/746 (97.99%), Query Frame = 0


Query: 33  MTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMR 92
           M +AGNNASSSLIFKLV+LN VCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMR
Sbjct: 1   MIVAGNNASSSLIFKLVVLNFVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMR 60

Query: 93  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 152
           MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAK+GVVSVFPNEKKHPHTTRS
Sbjct: 61  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKHPHTTRS 120

Query: 153 WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRC 212
           WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSD GYGPPPAKWKG CQ STNFRC
Sbjct: 121 WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDEGYGPPPAKWKGACQTSTNFRC 180

Query: 213 NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV 272
           NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV
Sbjct: 181 NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV 240

Query: 273 PSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS 332
           PSARIAVYK+CWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS
Sbjct: 241 PSARIAVYKICWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS 300

Query: 333 MKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTF 392
           MKNGILTSNSAGNDGPDFLT+RNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTF
Sbjct: 301 MKNGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTF 360

Query: 393 DLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFS 452
           DLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSI+SPSTFASFS
Sbjct: 361 DLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIVSPSTFASFS 420

Query: 453 SAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAP 512
           SAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAP
Sbjct: 421 SAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAP 480

Query: 513 IVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMS 572
           IVVSFSSRGPNP TLDILKPDLTAPGVEILAAWPPDAPVS GIRDSRSTLYNIISGTSMS
Sbjct: 481 IVVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPDAPVSSGIRDSRSTLYNIISGTSMS 540

Query: 573 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPG 632
           CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV LNPQAEFAYGAGHIDPLKATNPG
Sbjct: 541 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKATNPG 600

Query: 633 LVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI 692
           LVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI
Sbjct: 601 LVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI 660

Query: 693 NQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKT 752
           NQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPP LSFNGIGDKKSFTLTIDGTII+T
Sbjct: 661 NQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGTIIQT 720

Query: 753 IVSASVVWSDGSHNVRSPITIYIVNK 779
           IVSASVVWSDGSHNV     +Y+ NK
Sbjct: 721 IVSASVVWSDGSHNV---YIVYLGNK 743

[Close]

BLAST of CmaCh04G001840 vs. NCBI nr
Match: KAG7030696.1 (hypothetical protein SDJN02_04733, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1442.2 bits (3732), Expect = 0.0e+00
Identity = 717/733 (97.82%), Postives = 726/733 (99.05%), Query Frame = 0


Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRML 94
           MAGNNASSSLIFKLV+LNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRML
Sbjct: 1   MAGNNASSSLIFKLVVLNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRML 60

Query: 95  EETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWD 154
           EETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAK+GVVSVFPNEKKHPHTTRSWD
Sbjct: 61  EETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKHPHTTRSWD 120

Query: 155 FMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNK 214
           FMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSD GYGPPPAKWKG CQ STNFRCNK
Sbjct: 121 FMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDEGYGPPPAKWKGACQTSTNFRCNK 180

Query: 215 KIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPS 274
           KIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPS
Sbjct: 181 KIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPS 240

Query: 275 ARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMK 334
           ARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMK
Sbjct: 241 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMK 300

Query: 335 NGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDL 394
           NGILTSNSAGNDGPDFLT+RNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDL
Sbjct: 301 NGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDL 360

Query: 395 QQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSA 454
           QQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKIL+CDSI+SPSTFASFSSA
Sbjct: 361 QQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILICDSIVSPSTFASFSSA 420

Query: 455 VGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIV 514
           VGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIV
Sbjct: 421 VGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIV 480

Query: 515 VSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCP 574
           VSFSSRGPNP TLDILKPDLTAPGVEILAAWPP+APVS GIRDSRSTLYNIISGTSMSCP
Sbjct: 481 VSFSSRGPNPETLDILKPDLTAPGVEILAAWPPNAPVSSGIRDSRSTLYNIISGTSMSCP 540

Query: 575 HATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLV 634
           HATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLV
Sbjct: 541 HATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLV 600

Query: 635 YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQ 694
           YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQ
Sbjct: 601 YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQ 660

Query: 695 FFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIV 754
           FFTRTLTNVDTEASTYTSKILGAPEGLTITVDPP LSFNGIGDKKSFTLTIDGTII+TIV
Sbjct: 661 FFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGTIIQTIV 720

Query: 755 SASVVWSDGSHNV 768
           SASVVWSDGSHN+
Sbjct: 721 SASVVWSDGSHNL 733

[Close]

BLAST of CmaCh04G001840 vs. NCBI nr
Match: XP_022995146.1 (cucumisin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 712/751 (94.81%), Postives = 732/751 (97.47%), Query Frame = 0


Query: 29  MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS 88
           MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS
Sbjct: 1   MFPVMTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPS 60

Query: 89  HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH 148
           HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH
Sbjct: 61  HHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPH 120

Query: 149 TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST 208
           TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST
Sbjct: 121 TTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQAST 180

Query: 209 NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA 268
           NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA
Sbjct: 181 NFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTA 240

Query: 269 RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG 328
           RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG
Sbjct: 241 RGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIG 300

Query: 329 AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFT 388
           AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQG+T
Sbjct: 301 AFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGYT 360

Query: 389 INTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTF 448
           INTFDLQ K +PLIYAG+APNISGGF+G++SR+CS+NSVDRNLVKGKILVCDSILSPSTF
Sbjct: 361 INTFDLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTF 420

Query: 449 ASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVND 508
           AS SSAVGVVMN+AGVKDNARSYPLPSSYL   AGN++K Y+GSNNFPTATIFKSN VND
Sbjct: 421 ASLSSAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVND 480

Query: 509 TSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISG 568
           T+APIVVSFSSRGPNP T DILKPDLTAPGVEILAAW P APVS GI+DSR+TLYNIISG
Sbjct: 481 TAAPIVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISG 540

Query: 569 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKA 628
           TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV LNPQAEFAYGAGHIDPLKA
Sbjct: 541 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKA 600

Query: 629 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 688
            NPGL+YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP
Sbjct: 601 MNPGLIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 660

Query: 689 SESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGT 748
           SESINQFFTRTLTNV +EASTYTSKILGAPEGLTITVDPPVLSFNGIG K+SFTLTI GT
Sbjct: 661 SESINQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGT 720

Query: 749 IIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           I ++IVSASVVWSDGSHNVRSPITIYIVNKA
Sbjct: 721 ISQSIVSASVVWSDGSHNVRSPITIYIVNKA 751

[Close]

BLAST of CmaCh04G001840 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 645.6 bits (1664), Expect = 5.2e-185
Identity = 340/705 (48.23%), Postives = 457/705 (64.82%), Query Frame = 0


Query: 76  LGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDG 135
           +G   E   S PSHH+ +L++ +G+  A   L+ SYKRSFNGF   L++ E+QK+     
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 136 VVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGP 195
           VVSVFP++     TTRSWDF+GF     R    ESD++VGV+D+GIWPES SF D G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 196 PPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLV 255
           PP KWKG+C+    F CN K+IGAR Y   N F      S RD EGHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY---NKFA----DSARDEEGHGTHTASTAAGNAV 180

Query: 256 SQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGN 315
             AS +GLA GTARGGVPSARIA YKVC+ + C D DILAAFDDAIADGVD+IS+S+  +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 316 EPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSK 375
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  +V N SPW ++VAAS  DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 376 VQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGK 435
           V LGN     G ++NTF+L   +FP++Y     N+S   + A + +CS   VD  LVKGK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 436 ILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNF 495
           I++CD  L     A  + A+GV++ +  + D+A   P P+S LG     +IK+Y+ S   
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 496 PTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGI 555
           P A I ++  + D  AP V SFSSRGP+ V  ++LKPD++APG+EILAA+ P A  S  +
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 556 --RDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQ 615
              D RS  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTA P+N+  NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 616 AEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGR 675
            EFAYG+G I+P KA++PGLVY+    DY+  LC +G+ +  +   +G    C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 676 VWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFN 735
           V DLNYP+     +  +  N  F RT+TNV    STY + ++     L I+++P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 736 GIGDKKSFTLTIDGTIIK--TIVSASVVWSDGSHNVRSPITIYIV 777
            + +KKSF +TI G  +K  + VS+SVVWSDGSH+VRSPI  Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

[Close]

BLAST of CmaCh04G001840 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 634.4 bits (1635), Expect = 1.2e-181
Identity = 348/750 (46.40%), Postives = 480/750 (64.00%), Query Frame = 0


Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTP-SHHMRM 94
           MA   ASSSL+  L++L L      SS  +  D ++VYIVY+G+       TP S HM +
Sbjct: 1   MATLAASSSLLSCLLVLFL------SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNI 60

Query: 95  LEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSW 154
           L+E  G +     L+ SYKRSFNGF  RLTE E ++++   GVVSVFPN+K    TT SW
Sbjct: 61  LQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSW 120

Query: 155 DFMGFTTNV--PRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFR 214
           DFMG    +   R   VESD ++GV+D+GI PES SFSD G+GPPP KWKG C    NF 
Sbjct: 121 DFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT 180

Query: 215 CNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGG 274
           CN K+IGAR Y S+           RD +GHGTHTAST AG  V  AS FG+  GT RGG
Sbjct: 181 CNNKLIGARDYTSEGT---------RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGG 240

Query: 275 VPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFH 334
           VP++R+A YKVC   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH
Sbjct: 241 VPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFH 300

Query: 335 SMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINT 394
           +M  G+LT NSAGN GP  ++V   +PW L+VAAS+ +R  V+KV LGN     G ++N 
Sbjct: 301 AMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNA 360

Query: 395 FDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASF 454
           ++++ K +PL+Y  +A   S      ++  C  + VD++ VKGKILVC          S 
Sbjct: 361 YEMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV 420

Query: 455 SSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSA 514
             AVG++       D A  +PLP++ L T    ++ +Y+ S + P A + K+ A+ + ++
Sbjct: 421 -GAVGLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTS 480

Query: 515 PIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSM 574
           P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P    S    D+R   Y+++SGTSM
Sbjct: 481 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSM 540

Query: 575 SCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN---VGLNPQAEFAYGAGHIDPLKA 634
           SCPH    A YVKTF+P WSP+ I+SA+MTTA P+N    G+    EFAYG+GH+DP+ A
Sbjct: 541 SCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGI-ASTEFAYGSGHVDPIAA 600

Query: 635 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 694
           +NPGLVY+ +++D++ FLCG  Y++ +++ ++G+   C+ A      +LNYPS +   + 
Sbjct: 601 SNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSG 660

Query: 695 S-ESINQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLTID 754
           S  +    F RTLTNV T  STYTSK++ G    L + + P VLSF  + +K+SFT+T+ 
Sbjct: 661 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 720

Query: 755 GTIIKTIV--SASVVWSDGSHNVRSPITIY 775
           G+ + + V  SA+++WSDG+HNVRSPI +Y
Sbjct: 721 GSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

[Close]

BLAST of CmaCh04G001840 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 624.8 bits (1610), Expect = 9.4e-179
Identity = 336/735 (45.71%), Postives = 472/735 (64.22%), Query Frame = 0


Query: 53  LVCCLLASSFDSGNDG-RKVYIVYLGNKREDTASTP-SHHMRMLEETIGSTFAPEALLHS 112
           LV  L + S D  + G ++VYIVYLG+       TP S HM +L+E  G +     L+ S
Sbjct: 15  LVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRS 74

Query: 113 YKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNV--PRVKQV 172
           YK+SFNGF  RLTE E ++++  + VVSVFP+ K    TT SW+FMG    +   R + +
Sbjct: 75  YKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSI 134

Query: 173 ESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNF 232
           ESD ++GV+D+GI+PES SFSD G+GPPP KWKGTC    NF CN K+IGAR Y + +  
Sbjct: 135 ESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKS-- 194

Query: 233 PPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGC 292
             +  ++ RD  GHGTHTAS  AG  V+ ++ +GL  GTARGGVP+ARIAVYKVC ++GC
Sbjct: 195 --KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGC 254

Query: 293 EDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGP 352
           +   +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP
Sbjct: 255 DGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGP 314

Query: 353 DFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAP 412
              TV + +PW  SVAAS  +R  ++KV LG+  I  G ++NT+D+    +PL+Y  +A 
Sbjct: 315 KISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAA 374

Query: 413 NISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNA 472
             +     A  R C    +D  LVKGKI++CDS           +   +V N    +   
Sbjct: 375 LSTCSVDKA--RLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFI 434

Query: 473 RSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLD 532
           RS+P+  S+L      ++ +YM S   P AT+ KS  +++  AP+V SFSSRGP+ +  D
Sbjct: 435 RSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSD 494

Query: 533 ILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHP 592
           ILKPD+TAPGVEILAA+ PD+  +    D+R   Y+++SGTSM+CPH    A YVKTFHP
Sbjct: 495 ILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHP 554

Query: 593 TWSPAAIKSALMTTAIPLNVGLN--PQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFL 652
            WSP+ I+SA+MTTA P+N   +     EFAYG+GH+DP+ A NPGLVY+  + D++NFL
Sbjct: 555 QWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFL 614

Query: 653 CGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESINQFFTRTLTNVDT 712
           CG  Y++  +R ++GD S CT   S  +  +LNYP+ +   + ++  N  F RT+TNV  
Sbjct: 615 CGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGM 674

Query: 713 EASTYTSKILGAP-EGLTITVDPPVLSFNGIGDKKSFTLTIDGTII--KTIVSASVVWSD 772
           + STY +K++  P   L+I V P VLS   + +K+SF +T+    I  K  VSA+++WSD
Sbjct: 675 QKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSD 734

Query: 773 GSHNVRSPITIYIVN 778
           G+HNVRSPI +Y ++
Sbjct: 735 GTHNVRSPIIVYAMS 741

[Close]

BLAST of CmaCh04G001840 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 623.2 bits (1606), Expect = 2.7e-178
Identity = 345/754 (45.76%), Postives = 478/754 (63.40%), Query Frame = 0


Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLG--NKREDTASTPSHHMR 94
           MA   AS+ L   L++L     LL+S     ++  +VYIVY+G  + R D   T S HM 
Sbjct: 1   MANLAASTCLYSWLLVL-----LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPT-SDHMS 60

Query: 95  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 154
           +L++  G +     L+ SYKRSFNGF  RLTE E   I+  +GVVSVFPN+    HTT S
Sbjct: 61  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120

Query: 155 WDFMGFT--TNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNF 214
           WDFMG     N  R   +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C    NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180

Query: 215 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 274
            CN K+IGAR Y S+           RD+ GHGTHTAST AG  V   S FG+  GT RG
Sbjct: 181 TCNNKLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 240

Query: 275 GVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 334
           GVP++RIA YKVC   GC    +L++FDDAIADGVD+I++S+G   P  + +D IAIGAF
Sbjct: 241 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 300

Query: 335 HSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 394
           H+M  GILT +SAGN GP   TV + +PW  +VAAS+ +R  ++KV LGN     G ++N
Sbjct: 301 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 360

Query: 395 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFAS 454
            FD++ K++PL+Y  +A   S      T+  C+   ++++ VKGKILVC     PS +  
Sbjct: 361 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 420

Query: 455 FSSAVGVVMNDAGVK-DNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDT 514
             S   + + D   + D A ++ LP+S L      ++ +Y+ S + P A + K+  + + 
Sbjct: 421 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 480

Query: 515 SAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGT 574
           ++P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P+   S    D+R   Y++ SGT
Sbjct: 481 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 540

Query: 575 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV---GLNPQAEFAYGAGHIDPL 634
           SM+CPH    A YVKTF+P WSP+ I+SA+MTTA P+     G+    EFAYGAGH+DP+
Sbjct: 541 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGI-ASTEFAYGAGHVDPM 600

Query: 635 KATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALST 694
            A NPGLVY+ ++ D++ FLCG  Y++  ++ ++GD   C+  N     +LNYPS +   
Sbjct: 601 AALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKL 660

Query: 695 TPSES-INQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLT 754
           + ++S  +  F RTLTNV T  STY SK++ G    L+I V P VL F  + +K+SF++T
Sbjct: 661 SGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVT 720

Query: 755 IDGTIIKTIV--SASVVWSDGSHNVRSPITIYIV 777
           + G+ + + V  SA+++WSDG+HNVRSPI +YI+
Sbjct: 721 VTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731

[Close]

BLAST of CmaCh04G001840 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 621.7 bits (1602), Expect = 8.0e-178
Identity = 343/751 (45.67%), Postives = 475/751 (63.25%), Query Frame = 0


Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLG--NKREDTASTPSHHMR 94
           MA   AS+ L   L++L     LL+S     ++  +VYIVY+G  + R D   T S HM 
Sbjct: 1   MANLAASTCLYSWLLVL-----LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPT-SDHMS 60

Query: 95  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 154
           +L++  G +     L+ SYKRSFNGF  RLTE E   I+  +GVVSVFPN+    HTT S
Sbjct: 61  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120

Query: 155 WDFMGFT--TNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNF 214
           WDFMG     N  R   +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C    NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180

Query: 215 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 274
            CN K+IGAR Y S+           RD+ GHGTHTAST AG  V   S FG+  GT RG
Sbjct: 181 TCNNKLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 240

Query: 275 GVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 334
           GVP++RIA YKVC   GC    +L++FDDAIADGVD+I++S+G   P  + +D IAIGAF
Sbjct: 241 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 300

Query: 335 HSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 394
           H+M  GILT +SAGN GP   TV + +PW  +VAAS+ +R  ++KV LGN     G ++N
Sbjct: 301 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 360

Query: 395 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFAS 454
            FD++ K++PL+Y  +A   S      T+  C+   ++++ VKGKILVC     PS +  
Sbjct: 361 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 420

Query: 455 FSSAVGVVMNDAGVK-DNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDT 514
             S   + + D   + D A ++ LP+S L      ++ +Y+ S + P A + K+  + + 
Sbjct: 421 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 480

Query: 515 SAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGT 574
           ++P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P+   S    D+R   Y++ SGT
Sbjct: 481 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 540

Query: 575 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKAT 634
           SM+CPH    A YVKTF+P WSP+ I+SA+MTTA    +      EFAYGAGH+DP+ A 
Sbjct: 541 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAKGRGIA---STEFAYGAGHVDPMAAL 600

Query: 635 NPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPS 694
           NPGLVY+ ++ D++ FLCG  Y++  ++ ++GD   C+  N     +LNYPS +   + +
Sbjct: 601 NPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGT 660

Query: 695 ES-INQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLTIDG 754
           +S  +  F RTLTNV T  STY SK++ G    L+I V P VL F  + +K+SF++T+ G
Sbjct: 661 DSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG 720

Query: 755 TIIKTIV--SASVVWSDGSHNVRSPITIYIV 777
           + + + V  SA+++WSDG+HNVRSPI +YI+
Sbjct: 721 SDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 726

The following BLAST results are available for this feature:

BLAST of CmaCh04G001840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	7.3e-269	64.63	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIF8	3.7e-188	48.09	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]
Q9FIG2	1.7e-180	46.40	Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2...	[more]
Q9FGU3	1.3e-177	45.71	Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S...	[more]
Q8L7D2	3.9e-177	45.76	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]

BLAST of CmaCh04G001840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQ02	0.0e+00	97.95	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447181 PE=3 SV=1	[more]
A0A6J1K168	0.0e+00	94.81	cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1	[more]
A0A6J1FVT5	0.0e+00	89.84	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447322 PE=3 SV=1	[more]
A0A6J1K719	0.0e+00	88.16	cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1	[more]
A0A6J1FV97	0.0e+00	84.35	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1	[more]

BLAST of CmaCh04G001840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941974.1	0.0e+00	97.95	cucumisin-like [Cucurbita moschata]	[more]
XP_023546659.1	0.0e+00	97.20	cucumisin-like [Cucurbita pepo subsp. pepo]	[more]
KAG6600027.1	0.0e+00	96.78	hypothetical protein SDJN03_05260, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7030696.1	0.0e+00	97.82	hypothetical protein SDJN02_04733, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022995146.1	0.0e+00	94.81	cucumisin-like isoform X2 [Cucurbita maxima]	[more]

BLAST of CmaCh04G001840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59190.1	5.2e-185	48.23	subtilase family protein	[more]
AT5G59120.1	1.2e-181	46.40	subtilase 4.13	[more]
AT5G59100.1	9.4e-179	45.71	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59090.1	2.7e-178	45.76	subtilase 4.12	[more]
AT5G59090.2	8.0e-178	45.67	subtilase 4.12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 238..251 score: 51.7 coord: 567..583 score: 43.08 coord: 169..188 score: 33.68
None	No IPR available	GENE3D	2.60.40.2310		coord: 646..776 e-value: 1.2E-38 score: 133.7
None	No IPR available	GENE3D	3.50.30.30		coord: 369..507 e-value: 2.6E-187 score: 625.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 227..247
None	No IPR available	PANTHER	PTHR10795:SF649	PUTATIVE-RELATED	coord: 59..774
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 152..629 score: 26.152035
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 376..500 e-value: 5.72472E-15 score: 70.1351
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 170..621 e-value: 8.3E-46 score: 156.5
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 676..773 e-value: 1.2E-23 score: 83.1
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 57..148 e-value: 2.4E-25 score: 90.8
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 171..642 e-value: 2.6E-187 score: 625.2
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 147..633
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 71..144 e-value: 1.5E-14 score: 54.4
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 59..774
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 568..578
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 145..604 e-value: 3.55924E-129 score: 385.028

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G001840.1	CmaCh04G001840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity