CmaCh03G013990 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G013990
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	gibberellin 2-beta-dioxygenase
Location	Cma_Chr03: 8817543 .. 8818816 (+)
RNA-Seq Expression	CmaCh03G013990
Synteny	CmaCh03G013990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCGCCATGGTTGTTCTATCTCAGCCAGCGTTGGATTTGGAGAAATATTCTCTTCTCCGATCAACGGGCAAACCCGCCGCCTCCGCAGCCGATATTCCGGAAATTGACCTTTCAGACCCCAACGCTAAGTCCCACGTCGTCAAAGCCTGCGAAGAATTCGGGTTCTTCAAGCTGGTAAACCACCGGGTTCCCGTCGAGTTGATGACCAAACTCGAAGATGAATCCCTCTGTTTTTTCAAGCTTTCTCAGTCCGAGAAAGATAAAGCTCGCCCGCCTGACCCTTTGGGCTATGGCTCCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATCGAATATCTCCTTCTCAACGCTAATCCTCTCCCCCTTTTCTCCCAACAGTCCGATTCTTTCCTGTAAGTTGCCAATATTTAAAGCAATCTGTTTCGGAATTACAGAGTTCAAGCCCCTGTTCTTAGCCTTTTAAGATTTTGATTCTGATAATTTTAAATTTTTTTGCTGATTCTGTTGTAGCTCGGCGGCGGCGGAGTATGTGACGGCGGTGAAGAAATTGAGCGGCGAAGTGGTGGAATTAATAACAGAAGGATTAAAAATCGAGCCAAAAAATGCGATAAGCAGGCTTATAAACGACGAGAAAGCTGATTGTTATTTCAGAGTGAACCATTACCCGCCATGTCCGGAAATGCAAGCTGTTAGTGGACGAAACATGATCGGATTCGGGGAACATACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCAACCGGCTTGCAAATCTGCCGGAGAGACGGCGTGTGGGTCTCAGTGCCGCCAGATACGACGGCGTTCTTTGTCAATGTCGGCGATGCGCTGCAGGTGATGACCAACGGGAGATTCAAAAGCGTGAAGCACAAAGTGGTGGCGGATTCCAGCAAAGAGAGGGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTCGGCCATTGTCGCAGGTTTTAGCAGAGGGGGAAGAAAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTTTAAGACCAGATTGGCTGATTACAGGCTCGGTGCGTTTGAAAAGAGTCAAAAGTCTACGTTTTAAACTTGTTTCGTTGACCCAACTTTATAATTCTGTTCCATAAATGGTAACTCGAGCTTTTTTTCTTGTTGACGAAGGAACTATTCGATGACGTTCTGGTCGGTAGTTTTATTATTTATATAATCCAACCTCTATTAATAAAATACGTATTAATTAT

mRNA sequence

GCCGCCATGGTTGTTCTATCTCAGCCAGCGTTGGATTTGGAGAAATATTCTCTTCTCCGATCAACGGGCAAACCCGCCGCCTCCGCAGCCGATATTCCGGAAATTGACCTTTCAGACCCCAACGCTAAGTCCCACGTCGTCAAAGCCTGCGAAGAATTCGGGTTCTTCAAGCTGGTAAACCACCGGGTTCCCGTCGAGTTGATGACCAAACTCGAAGATGAATCCCTCTGTTTTTTCAAGCTTTCTCAGTCCGAGAAAGATAAAGCTCGCCCGCCTGACCCTTTGGGCTATGGCTCCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATCGAATATCTCCTTCTCAACGCTAATCCTCTCCCCCTTTTCTCCCAACAGTCCGATTCTTTCCTCTCGGCGGCGGCGGAGTATGTGACGGCGGTGAAGAAATTGAGCGGCGAAGTGGTGGAATTAATAACAGAAGGATTAAAAATCGAGCCAAAAAATGCGATAAGCAGGCTTATAAACGACGAGAAAGCTGATTGTTATTTCAGAGTGAACCATTACCCGCCATGTCCGGAAATGCAAGCTGTTAGTGGACGAAACATGATCGGATTCGGGGAACATACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCAACCGGCTTGCAAATCTGCCGGAGAGACGGCGTGTGGGTCTCAGTGCCGCCAGATACGACGGCGTTCTTTGTCAATGTCGGCGATGCGCTGCAGGTGATGACCAACGGGAGATTCAAAAGCGTGAAGCACAAAGTGGTGGCGGATTCCAGCAAAGAGAGGGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTCGGCCATTGTCGCAGGTTTTAGCAGAGGGGGAAGAAAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTTTAAGACCAGATTGGCTGATTACAGGCTCGGTGCGTTTGAAAAGAGTCAAAAGTCTACGTTTTAAACTTGTTTCGTTGACCCAACTTTATAATTCTGTTCCATAAATGGTAACTCGAGCTTTTTTTCTTGTTGACGAAGGAACTATTCGATGACGTTCTGGTCGGTAGTTTTATTATTTATATAATCCAACCTCTATTAATAAAATACGTATTAATTAT

Coding sequence (CDS)

ATGGTTGTTCTATCTCAGCCAGCGTTGGATTTGGAGAAATATTCTCTTCTCCGATCAACGGGCAAACCCGCCGCCTCCGCAGCCGATATTCCGGAAATTGACCTTTCAGACCCCAACGCTAAGTCCCACGTCGTCAAAGCCTGCGAAGAATTCGGGTTCTTCAAGCTGGTAAACCACCGGGTTCCCGTCGAGTTGATGACCAAACTCGAAGATGAATCCCTCTGTTTTTTCAAGCTTTCTCAGTCCGAGAAAGATAAAGCTCGCCCGCCTGACCCTTTGGGCTATGGCTCCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATCGAATATCTCCTTCTCAACGCTAATCCTCTCCCCCTTTTCTCCCAACAGTCCGATTCTTTCCTCTCGGCGGCGGCGGAGTATGTGACGGCGGTGAAGAAATTGAGCGGCGAAGTGGTGGAATTAATAACAGAAGGATTAAAAATCGAGCCAAAAAATGCGATAAGCAGGCTTATAAACGACGAGAAAGCTGATTGTTATTTCAGAGTGAACCATTACCCGCCATGTCCGGAAATGCAAGCTGTTAGTGGACGAAACATGATCGGATTCGGGGAACATACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCAACCGGCTTGCAAATCTGCCGGAGAGACGGCGTGTGGGTCTCAGTGCCGCCAGATACGACGGCGTTCTTTGTCAATGTCGGCGATGCGCTGCAGGTGATGACCAACGGGAGATTCAAAAGCGTGAAGCACAAAGTGGTGGCGGATTCCAGCAAAGAGAGGGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTCGGCCATTGTCGCAGGTTTTAGCAGAGGGGGAAGAAAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTTTAAGACCAGATTGGCTGATTACAGGCTCGGTGCGTTTGAAAAGAGTCAAAAGTCTACGTTTTAA

Protein sequence

MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKEFTWWEYKTAAFKTRLADYRLGAFEKSQKSTF

Homology

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BLAST of CmaCh03G013990 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.3e-115
Identity = 207/333 (62.16%), Postives = 255/333 (76.58%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPAL+  ++ LL+   K       IP +DL+ P+AK+ +V AC +FGFFKLVNH 
Sbjct: 1   MVVLSQPALN--QFFLLKPF-KSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHG 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP-- 120
           VP+ELM  LE+E+L FFK SQSEKD+A PPDP GYGSK IG NGD GW+EYLLLN NP  
Sbjct: 61  VPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDV 120

Query: 121 -----LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKAD 180
                L +F +    F +    Y+TAVK +   V+EL+ EGL I  +N +SRL+ DEK+D
Sbjct: 121 ISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSD 180

Query: 181 CYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPD 240
             FR+NHYPPCPE+QA++ RN++GFGEHTDPQIIS+LRSN+++GLQIC  DG WVSVPPD
Sbjct: 181 SCFRLNHYPPCPEVQALN-RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPD 240

Query: 241 TTAFFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEG 300
            T+FF+NVGDALQVMTNGRFKSVKH+V+AD++K R+SMIYFGGP LSE I PL  V+ +G
Sbjct: 241 QTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKG 300

Query: 301 EESLYKEFTWWEYKTAAFKTRLADYRLGAFEKS 327
           EE LYKEFTW EYK AA+ +RLAD RL  F+KS
Sbjct: 301 EECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

[Close]

BLAST of CmaCh03G013990 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-105
Identity = 183/328 (55.79%), Postives = 252/328 (76.83%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MV+LS+P    E+Y+ +R+   P   ++ IP +DLS P+AK+ +VKACE+FGFFK++NH 
Sbjct: 1   MVLLSKPT--SEQYTYVRN-NMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHG 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP-- 120
           +P++ +++LE E+  FF L Q+EK+KA P +P GYG+K IG NGD GWIEYLLL  N   
Sbjct: 61  IPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDH 120

Query: 121 -LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFR 180
              L+ +  D F     +Y  A++ ++ E+++L+ EGLKI+PKN  S+L+ D+++DC FR
Sbjct: 121 NFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFR 180

Query: 181 VNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAF 240
           VNHYP CPE+ A++G N+IGFGEHTDPQIIS+LRSNN++G QI  RDG W+SVPPD ++F
Sbjct: 181 VNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSF 240

Query: 241 FVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESL 300
           F+NVGD+LQVMTNGRFKSV+H+V+A+    R+SMIYF GPPLSEKI PL   L +G+ESL
Sbjct: 241 FINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPS-LMKGKESL 300

Query: 301 YKEFTWWEYKTAAFKTRLADYRLGAFEK 326
           YKEFTW+EYK++ + +RLAD RLG +E+
Sbjct: 301 YKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of CmaCh03G013990 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-103
Identity = 187/334 (55.99%), Postives = 247/334 (73.95%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGK--PAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60
           MVVL QP + L+ +  L  T K  P  ++  IP ++L+DP AK+ +VKACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L QS K++A PP+P GYG+K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEK 180
                    +F Q    F  +  EY+  +K++S +V+E++ E L IEP++ +S+++ DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVP 240
           +D   R+NHYP   E         +GFGEHTDPQIIS+LRSNN+ GLQIC +DG WV+VP
Sbjct: 181 SDSCLRLNHYPAAEE--EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDTTAFFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLA 300
           PD ++FF+NVGDALQVMTNGRFKSVKH+V+AD+ + R+SMIYFGGPPLS+KI PL  ++ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAFKTRLADYRLGAFEK 326
           E ++ LYKEFTW +YK++A+K++L DYRLG FEK
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of CmaCh03G013990 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 4.9e-97
Identity = 178/333 (53.45%), Postives = 233/333 (69.97%), Query Frame = 0


Query: 1   MVVLSQPA-LDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNH 60
           MV++ QPA  D   Y   +   +P      IP IDL+D +AK+ +VKACEEFGFFK++NH
Sbjct: 1   MVIVLQPASFDSNLYVNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVINH 60

Query: 61  RVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNAN-- 120
            V  +L+T+LE E++ FF L  S KDKA PPDP GYG+K IG NGD GW+EY+LLNAN  
Sbjct: 61  GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLC 120

Query: 121 -----PLPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKA 180
                   +F      F  A  EY+  +K++S + +E++ E LKIEPK  +SRL+  +++
Sbjct: 121 LESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKES 180

Query: 181 DCYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPP 240
           D   R+NHY   PE +    +  IGFGEHTDPQ+ISLLRSN++ GLQIC +DG WV V P
Sbjct: 181 DSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTP 240

Query: 241 DTTAFFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAE 300
           D ++FFV VGD LQVMTNGRFKSVKH+VV ++ + R+SMIYF GPPLSEKI PLS ++ +
Sbjct: 241 DHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPK 300

Query: 301 GEESLYKEFTWWEYKTAAFKTRLADYRLGAFEK 326
            ++ LY EFTW +YK +A+KT+L DYRLG FEK
Sbjct: 301 QDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of CmaCh03G013990 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-93
Identity = 178/329 (54.10%), Postives = 229/329 (69.60%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVL+ P   ++   LLRS   P    + +P +DL  P A   VV ACE +GFFK+VNH 
Sbjct: 1   MVVLAGPPA-VDHIPLLRSP-DPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHG 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           V  + M K E E++ FF  +Q +KD++ P  P GYGSK IG NGD GW+EYLLL  +   
Sbjct: 61  VATDTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDAS 120

Query: 121 LFS----QQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYF 180
           L           F +A  EY++ V+K++  V+E ++EGL I   +A+S L+  E +D  F
Sbjct: 121 LADACTVPSCAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVF 180

Query: 181 RVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTA 240
           RVNHYPPC  +Q + G ++ GFGEHTDPQ++S+LRSN ++GLQI  RDG WVSVP D  +
Sbjct: 181 RVNHYPPCRALQGL-GCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDS 240

Query: 241 FFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEES 300
           FFVNVGD+LQV+TNGRFKSVKH+VVA+S K RVS IYFGGPPL+++I PL Q+L EGE+S
Sbjct: 241 FFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQS 300

Query: 301 LYKEFTWWEYKTAAFKTRLADYRLGAFEK 326
           LYKEFTW EYK AA+K+RL D RL  FEK
Sbjct: 301 LYKEFTWDEYKKAAYKSRLGDNRLAQFEK 326

[Close]

BLAST of CmaCh03G013990 vs. ExPASy TrEMBL
Match: A0A6J1IJN1 (gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 9.6e-189
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of CmaCh03G013990 vs. ExPASy TrEMBL
Match: I0J1J0 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 2.8e-188
Identity = 330/331 (99.70%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of CmaCh03G013990 vs. ExPASy TrEMBL
Match: A0A6J1EDN7 (gibberellin 2-beta-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111433225 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.1e-183
Identity = 321/331 (96.98%), Postives = 324/331 (97.89%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQP LDLEKYS+LRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPELDLEKYSVLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           +FSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDEKADCYFRVNH
Sbjct: 121 IFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLAD RLGAFEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTRLADNRLGAFEKSQNSTF 331

[Close]

BLAST of CmaCh03G013990 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.0e-161
Identity = 278/331 (83.99%), Postives = 306/331 (92.45%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPAL L++YSLLR+T KP AS  +IPEIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLE+E+LCFFKLSQSEKDKARPPDPLGYG KNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           +FSQ S+SFL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240
           YPPCP++QA+  SGRNMIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  A F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVADS++ERVSMIYFGGPPLSEKI PL +VL +GEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 330
           KEFTWWEYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of CmaCh03G013990 vs. ExPASy TrEMBL
Match: A0A5A7UGB1 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009030 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.7e-159
Identity = 278/330 (84.24%), Postives = 299/330 (90.61%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQP LDL +YSLLRST KP AS ADIPEIDLSDPNAK H+VKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLS+SEKDKARPP PLGYGSKNIGSNGDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           +FS     FL AA EYVTAVKKLS EVVELI EGLK+E +NAIS+L+NDEKADC FRVNH
Sbjct: 121 IFSH---DFLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQ +SGRNMIGFGEHTDPQIIS+LRSNNSTGLQIC RDG WVSVPPD  A FVN
Sbjct: 181 YPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVV D ++ERVSMIYFGGPPLSEKI PL ++L +GEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKST 331
           FTWWEYKTAA+K++LADYRLGAFEKS  S+
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSPISS 327

[Close]

BLAST of CmaCh03G013990 vs. NCBI nr
Match: XP_022977872.1 (gibberellin 2-beta-dioxygenase [Cucurbita maxima])

HSP 1 Score: 669.1 bits (1725), Expect = 2.0e-188
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of CmaCh03G013990 vs. NCBI nr
Match: CBL59329.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 667.5 bits (1721), Expect = 5.8e-188
Identity = 330/331 (99.70%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of CmaCh03G013990 vs. NCBI nr
Match: XP_022925964.1 (gibberellin 2-beta-dioxygenase [Cucurbita moschata])

HSP 1 Score: 651.4 bits (1679), Expect = 4.3e-183
Identity = 321/331 (96.98%), Postives = 324/331 (97.89%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQP LDLEKYS+LRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPELDLEKYSVLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           +FSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDEKADCYFRVNH
Sbjct: 121 IFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLAD RLGAFEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTRLADNRLGAFEKSQNSTF 331

[Close]

BLAST of CmaCh03G013990 vs. NCBI nr
Match: KAG6581428.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 649.0 bits (1673), Expect = 2.1e-182
Identity = 320/331 (96.68%), Postives = 323/331 (97.58%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           LFSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDE ADCYFRVNH
Sbjct: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKT+LAD RLG FEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 331

[Close]

BLAST of CmaCh03G013990 vs. NCBI nr
Match: XP_023545071.1 (gibberellin 2-beta-dioxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.5 bits (1669), Expect = 6.2e-182
Identity = 321/331 (96.98%), Postives = 323/331 (97.58%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180
           LFSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH
Sbjct: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YP CPEMQ V GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPWCPEMQDVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADS KERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSDKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 332
           FTWWEYKTAAFKTRLA+ RLGAFEKSQ S F
Sbjct: 301 FTWWEYKTAAFKTRLAENRLGAFEKSQNSAF 331

[Close]

BLAST of CmaCh03G013990 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-104
Identity = 187/334 (55.99%), Postives = 247/334 (73.95%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGK--PAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60
           MVVL QP + L+ +  L  T K  P  ++  IP ++L+DP AK+ +VKACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L QS K++A PP+P GYG+K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEK 180
                    +F Q    F  +  EY+  +K++S +V+E++ E L IEP++ +S+++ DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVP 240
           +D   R+NHYP   E         +GFGEHTDPQIIS+LRSNN+ GLQIC +DG WV+VP
Sbjct: 181 SDSCLRLNHYPAAEE--EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDTTAFFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLA 300
           PD ++FF+NVGDALQVMTNGRFKSVKH+V+AD+ + R+SMIYFGGPPLS+KI PL  ++ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAFKTRLADYRLGAFEK 326
           E ++ LYKEFTW +YK++A+K++L DYRLG FEK
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of CmaCh03G013990 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 355.9 bits (912), Expect = 3.5e-98
Identity = 178/333 (53.45%), Postives = 233/333 (69.97%), Query Frame = 0


Query: 1   MVVLSQPA-LDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNH 60
           MV++ QPA  D   Y   +   +P      IP IDL+D +AK+ +VKACEEFGFFK++NH
Sbjct: 1   MVIVLQPASFDSNLYVNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVINH 60

Query: 61  RVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNAN-- 120
            V  +L+T+LE E++ FF L  S KDKA PPDP GYG+K IG NGD GW+EY+LLNAN  
Sbjct: 61  GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLC 120

Query: 121 -----PLPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKA 180
                   +F      F  A  EY+  +K++S + +E++ E LKIEPK  +SRL+  +++
Sbjct: 121 LESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKES 180

Query: 181 DCYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPP 240
           D   R+NHY   PE +    +  IGFGEHTDPQ+ISLLRSN++ GLQIC +DG WV V P
Sbjct: 181 DSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTP 240

Query: 241 DTTAFFVNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAE 300
           D ++FFV VGD LQVMTNGRFKSVKH+VV ++ + R+SMIYF GPPLSEKI PLS ++ +
Sbjct: 241 DHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPK 300

Query: 301 GEESLYKEFTWWEYKTAAFKTRLADYRLGAFEK 326
            ++ LY EFTW +YK +A+KT+L DYRLG FEK
Sbjct: 301 QDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of CmaCh03G013990 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 342.8 bits (878), Expect = 3.0e-94
Identity = 167/312 (53.53%), Postives = 228/312 (73.08%), Query Frame = 0


Query: 23  PAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSQS 82
           P +  + IP ID+SDP +K  +VKACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNAN------PL-PLFSQQSDSFLSAAAE 142
           EK +     P GYG+  IG NGD GW+EYLL+NAN      PL P   +   +F +A  E
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNHYPPCP--EMQAVSGR 202
           Y T+V+K++ +V+E IT+GL I+P+N +S+L++D+  D   R+NHYPPCP    +   G+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIIS+LRSNN++GLQI   DG W+SVPPD T+FF NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKEFTWWEYKTAAFKT 322
           KSV+H+V+A+  K RVSMIYF GP L+++I PL+ ++   +E LY+EFTW EYK + + +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 RLADYRLGAFEK 326
           RL+D RL  FE+
Sbjct: 311 RLSDNRLQQFER 321

[Close]

BLAST of CmaCh03G013990 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 144/321 (44.86%), Postives = 203/321 (63.24%), Query Frame = 0


Query: 17  LRSTGKPAASAAD--IPEID--LSDPNAKSH-VVKACEEFGFFKLVNHRVPVELMTKLED 76
           L++TGK   S+ +   P ID  L+D +  S  +VKACE  GFFK++NH V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  ESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLPLFSQQSD---- 136
           E   FF   +S+K +A P  P GYG KNIG NGD G +EYLLL+ANP  +  +       
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 137 ---SFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNHYPPC 196
               F SA  +Y+  V+ L+ E+++L  E L  +  + +S LI D ++D   R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 197 PEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDA 256
           P   A+SG   IGFGEH+DPQI+++LRSN+  GL+IC RDG+W+ +P D T FFV VGD 
Sbjct: 189 P--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDC 248

Query: 257 LQVMTNGRFKSVKHKVVADSSKE-RVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKEFTW 316
           LQ +TNGRF SV+H+V+A+++K+ R+S +YF  PPL  KI PL ++++      Y  FTW
Sbjct: 249 LQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTW 308

Query: 317 WEYKTAAFKTRLADYRLGAFE 325
            +YK A +  RL   RL  F+
Sbjct: 309 GDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of CmaCh03G013990 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 136/256 (53.12%), Postives = 180/256 (70.31%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGK--PAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60
           MVVL QP + L+ +  L  T K  P  ++  IP ++L+DP AK+ +VKACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L QS K++A PP+P GYG+K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEK 180
                    +F Q    F  +  EY+  +K++S +V+E++ E L IEP++ +S+++ DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCYFRVNHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVP 240
           +D   R+NHYP   E         +GFGEHTDPQIIS+LRSNN+ GLQIC +DG WV+VP
Sbjct: 181 SDSCLRLNHYPAAEE--EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDTTAFFVNVGDALQV 248
           PD ++FF+NVGDALQV
Sbjct: 241 PDHSSFFINVGDALQV 253

The following BLAST results are available for this feature:

BLAST of CmaCh03G013990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	2.3e-115	62.16	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9SQ80	2.2e-105	55.79	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XFR9	1.6e-103	55.99	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
O64692	4.9e-97	53.45	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q8S0S6	1.1e-93	54.10	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of CmaCh03G013990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IJN1	9.6e-189	100.00	gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 ...	[more]
I0J1J0	2.8e-188	99.70	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1	[more]
A0A6J1EDN7	2.1e-183	96.98	gibberellin 2-beta-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111433225 PE=...	[more]
A0A2H4V3T7	5.0e-161	83.99	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
A0A5A7UGB1	4.7e-159	84.24	Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

BLAST of CmaCh03G013990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977872.1	2.0e-188	100.00	gibberellin 2-beta-dioxygenase [Cucurbita maxima]	[more]
CBL59329.1	5.8e-188	99.70	Gibberellin 2-oxidase [Cucurbita maxima]	[more]
XP_022925964.1	4.3e-183	96.98	gibberellin 2-beta-dioxygenase [Cucurbita moschata]	[more]
KAG6581428.1	2.1e-182	96.68	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023545071.1	6.2e-182	96.98	gibberellin 2-beta-dioxygenase [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh03G013990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	1.1e-104	55.99	gibberellin 2-oxidase	[more]
AT2G34555.1	3.5e-98	53.45	gibberellin 2-oxidase 3	[more]
AT1G78440.1	3.0e-94	53.53	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G02400.1	1.1e-72	44.86	gibberellin 2-oxidase 6	[more]
AT1G30040.2	1.2e-71	53.13	gibberellin 2-oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..116 e-value: 9.5E-13 score: 48.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..275 e-value: 6.2E-26 score: 90.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 10..317 e-value: 1.4E-76 score: 259.9
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..326
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 10..326
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 24..317
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 171..277 score: 11.968538

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G013990.1	CmaCh03G013990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding