CmaCh03G013510 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G013510
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	MACPF domain-containing protein
Location	Cma_Chr03: 8622443 .. 8623468 (+)
RNA-Seq Expression	CmaCh03G013510
Synteny	CmaCh03G013510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTATCCCGTCTCTTCTCCGGTTGCTGTTGCTTCCAATGTTCTCAATTTTGCTGCTTCAATGGCCACAACTCACAAAACCCAGGTTTTGTTTATTTGAAACTGACCCACTTCTTACCTTTTCTACATATGGACGCTTTGACCACTACTCTTTGCAAGTCCATCGAAGCTTTAGGGCGTGGTTTTGATGTCACCGCCGATATTCGTCTTCTTTACTGCAAGGGAACACCAGGCTCGAGGCTGGTTCACCTCAATGATACCCACACCAGGGATCTTGTTCTCTCTGATGGGCCTGTGGTTCCCAATGTGCCTGATGATGTTCAGTGTTCTTCAGAATCCAGGGTAACCGAAAACCTCCCTGTTTGCAGCTTCCACAAGGTGAATTCCCAGCTGTTCTTTCAATTGGGTCTTGTTTCTTTTTGCTTATGATTGACCCCTTTTGCCCCTTCTTGAACTTCCCTTCCTGAATGCTTGTGGATTCAAATGTCAGTTGAGTTTAAATGTTGTATATATCACATTGATGCCTGCAATTCCAGAAGGTGGAATGCTTCAATCAGACATCGCATATATCAGGAAACATTCCACTTGGAAGCTTTAATGCCATGTTCAATTCAGTGGTTCTTGCCAAGTTGATGCCGCAGCTACAAAATCACTTGTTATGATTGGATACTTCATTCCTCTCTTTAAGGTTGCATTGAAAAGCTCAAACTTAGTCTTACATGAGGATATCAAGCGTGCTGTGCCTTACACATGGGACCCTGTGTCTTTAAGGTTGCATTGAAATCGATTCCTTCTCCATCCACGCTACTGCACACTATAAAATTTTCATCCAACAAATTGCATCTTGTAGGCTTTAGTTAGTATTGCTTGATATGAAGACGTTGGTTTTCTTTCCAAAATTTTATTATCTGAATCTCAGTTATTCTCAAAGACAAGCCCCCTATCGAGGATCTGCAGTATTTTTTGGATTTTCAAATCGGTAGAGCATGGGCTCCTGAACAAACTAACCTGCAAACAAAATAA

mRNA sequence

ATGAAGCTATCCCGTCTCTTCTCCGGTTGCTGTTGCTTCCAATGTTCTCAATTTTGCTGCTTCAATGGCCACAACTCACAAAACCCAGGTTTTGTTTATTTGAAACTGACCCACTTCTTACCTTTTCTACATATGGACGCTTTGACCACTACTCTTTGCAAGTCCATCGAAGCTTTAGGGCGTGGTTTTGATGTCACCGCCGATATTCGTCTTCTTTACTGCAAGGGAACACCAGGCTCGAGGCTGGTTCACCTCAATGATACCCACACCAGGGATCTTGTTCTCTCTGATGGGCCTGTGGTTCCCAATGTGCCTGATGATGTTCAGTGTTCTTCAGAATCCAGGGTAACCGAAAACCTCCCTGTTTGCAGCTTCCACAAGGTGAATTCCCAGCTTGGTTCTTGCCAAGTTGATGCCGCAGCTACAAAATCACTTGTTATGATTGGATACTTCATTCCTCTCTTTAAGGTTGCATTGAAAAGCTCAAACTTAGTCTTACATGAGGATATCAAGCGTGCTGTGCCTTACACATGGGACCCTGTTGCATTGAAATCGATTCCTTCTCCATCCACGCTACTGCACACTATAAAATTTTCATCCAACAAATTGCATCTTGTAGGCTTTATTATTCTCAAAGACAAGCCCCCTATCGAGGATCTGCAGTATTTTTTGGATTTTCAAATCGGTAGAGCATGGGCTCCTGAACAAACTAACCTGCAAACAAAATAA

Coding sequence (CDS)

ATGAAGCTATCCCGTCTCTTCTCCGGTTGCTGTTGCTTCCAATGTTCTCAATTTTGCTGCTTCAATGGCCACAACTCACAAAACCCAGGTTTTGTTTATTTGAAACTGACCCACTTCTTACCTTTTCTACATATGGACGCTTTGACCACTACTCTTTGCAAGTCCATCGAAGCTTTAGGGCGTGGTTTTGATGTCACCGCCGATATTCGTCTTCTTTACTGCAAGGGAACACCAGGCTCGAGGCTGGTTCACCTCAATGATACCCACACCAGGGATCTTGTTCTCTCTGATGGGCCTGTGGTTCCCAATGTGCCTGATGATGTTCAGTGTTCTTCAGAATCCAGGGTAACCGAAAACCTCCCTGTTTGCAGCTTCCACAAGGTGAATTCCCAGCTTGGTTCTTGCCAAGTTGATGCCGCAGCTACAAAATCACTTGTTATGATTGGATACTTCATTCCTCTCTTTAAGGTTGCATTGAAAAGCTCAAACTTAGTCTTACATGAGGATATCAAGCGTGCTGTGCCTTACACATGGGACCCTGTTGCATTGAAATCGATTCCTTCTCCATCCACGCTACTGCACACTATAAAATTTTCATCCAACAAATTGCATCTTGTAGGCTTTATTATTCTCAAAGACAAGCCCCCTATCGAGGATCTGCAGTATTTTTTGGATTTTCAAATCGGTAGAGCATGGGCTCCTGAACAAACTAACCTGCAAACAAAATAA

Protein sequence

MKLSRLFSGCCCFQCSQFCCFNGHNSQNPGFVYLKLTHFLPFLHMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPNVPDDVQCSSESRVTENLPVCSFHKVNSQLGSCQVDAAATKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKSIPSPSTLLHTIKFSSNKLHLVGFIILKDKPPIEDLQYFLDFQIGRAWAPEQTNLQTK

Homology

[Close]

BLAST of CmaCh03G013510 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.9e-43
Identity = 114/318 (35.85%), Postives = 147/318 (46.23%), Query Frame = 0


Query: 46  DALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPNVP 105
           +ALTTTL  +I+ALGRGFDVT+D+RLLYCKG PGSRLV + +   RDL LS G ++PNVP
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVP 74

Query: 106 DDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAATK 165
            D+ CS  +  T+ + VCSFH++  +                       GS QVDAA+TK
Sbjct: 75  ADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTK 134

Query: 166 SLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL-------------------- 225
           SL ++GYFIPL+ V L    LVLH +I+RAVP +WDP +L                    
Sbjct: 135 SLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGR 194

Query: 226 --------KSIPSP---------------------STLLHTIKFSSNKL----------- 243
                   +S P P                      ++   +K+    +           
Sbjct: 195 DVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDD 254

[Close]

BLAST of CmaCh03G013510 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.1e-16
Identity = 51/168 (30.36%), Postives = 81/168 (48.21%), Query Frame = 0


Query: 39  FLPFLHMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTP-GSRLVHLNDTHTRDLVLSD 98
           F  F  +DA +    K++  +G G+D+ +D+R   CK TP GSRLV ++ T  RDL+   
Sbjct: 3   FNNFTRLDAHSAAE-KAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPG 62

Query: 99  GPVVPNVPDDVQCSSESRVTENLPVCSFHKVNSQLGSCQV-------------------- 158
           G VV NV   ++C    R      + SF++++ +                          
Sbjct: 63  GIVVNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCW 122

Query: 159 --DAAATKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL 184
             DA++ K+L   G+FI L+ V +    L L +++KR VP +WD  AL
Sbjct: 123 PKDASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAAL 169

[Close]

BLAST of CmaCh03G013510 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 6.9e-16
Identity = 53/153 (34.64%), Postives = 78/153 (50.98%), Query Frame = 0


Query: 55  SIEALGRGFDVTADIRLLYCK-GTPGSRLVHLNDTHTR-DLVLSDGPVVPNVPDDVQCSS 114
           +I ++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G  +PNV   ++C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 115 ESRVTENLPVCSFHKVNSQ---------------------LGSC-QVDAAATKSLVMIGY 174
             R+     +  F ++  Q                       SC Q DAA TK+L   G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 175 FIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL 184
           FI L+ VAL  S ++L E +K+AVP TWDP AL
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAAL 166

[Close]

BLAST of CmaCh03G013510 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.1e-13
Identity = 51/157 (32.48%), Postives = 74/157 (47.13%), Query Frame = 0


Query: 55  SIEALGRGFDVTADIRLLYCKGTPGS----RLVHLNDTHTRDLVLSDGPVVPNVPDDVQC 114
           ++++LG+GFD+TAD RL YCK   GS    RLV L+ T  R+L +    V  NV  D+ C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 115 SSESRVTENLPVCSFHKVNS-----------------------QLGSCQVDAAATKSLVM 174
               R      +  F+K++                        Q GS   DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 175 IGYFIPLFKVALKSSN-LVLHEDIKRAVPYTWDPVAL 184
               + LF + + + N L L + ++ AVP +WDP  L
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLL 168

[Close]

BLAST of CmaCh03G013510 vs. ExPASy TrEMBL
Match: A0A5D3CPE6 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008470 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.9e-62
Identity = 153/320 (47.81%), Postives = 169/320 (52.81%), Query Frame = 0


Query: 44  HMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPN 103
           +MDALTTTLC SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D HTRDLVLSDG VVPN
Sbjct: 18  NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPN 77

Query: 104 VPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAA 163
           VPDDVQCSS++R TEN+PVCSFHK+                          GS Q+DAAA
Sbjct: 78  VPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQLDAAA 137

Query: 164 TKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKS---------------- 223
           TKSL MIGY+IP FKVALKSSNLVL EDIKRAVPYTWDPV+L S                
Sbjct: 138 TKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 197

Query: 224 ------------------------------------------------------------ 243
                                                                       
Sbjct: 198 GRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKDVTVIFRRRGG 257

[Close]

BLAST of CmaCh03G013510 vs. ExPASy TrEMBL
Match: A0A1S3AZC7 (MACPF domain-containing protein CAD1 OS=Cucumis melo OX=3656 GN=LOC103484421 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.9e-62
Identity = 153/320 (47.81%), Postives = 169/320 (52.81%), Query Frame = 0


Query: 44  HMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPN 103
           +MDALTTTLC SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D HTRDLVLSDG VVPN
Sbjct: 18  NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPN 77

Query: 104 VPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAA 163
           VPDDVQCSS++R TEN+PVCSFHK+                          GS Q+DAAA
Sbjct: 78  VPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQLDAAA 137

Query: 164 TKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKS---------------- 223
           TKSL MIGY+IP FKVALKSSNLVL EDIKRAVPYTWDPV+L S                
Sbjct: 138 TKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 197

Query: 224 ------------------------------------------------------------ 243
                                                                       
Sbjct: 198 GRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKDVTVIFRRRGG 257

[Close]

BLAST of CmaCh03G013510 vs. ExPASy TrEMBL
Match: A0A0A0KKH0 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G520370 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.5e-61
Identity = 154/319 (48.28%), Postives = 168/319 (52.66%), Query Frame = 0


Query: 45  MDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPNV 104
           MDALTTTL  SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D H RDLVLSDG VVPNV
Sbjct: 1   MDALTTTLRNSIAALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHNRDLVLSDGVVVPNV 60

Query: 105 PDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAAT 164
           PDDVQCSS++R TEN+PVCSFHK+                          GS QVDAAAT
Sbjct: 61  PDDVQCSSDTRATENVPVCSFHKMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAAT 120

Query: 165 KSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKS----------------- 224
           KSL MIGY+IPLFKVALKSSNLVL EDIKRAVPYTWDPV+L S                 
Sbjct: 121 KSLAMIGYYIPLFKVALKSSNLVLCEDIKRAVPYTWDPVSLASFIENFGTHIITSATIGG 180

Query: 225 ------------------------------------------------------------ 243
                                                                       
Sbjct: 181 RDVVYIRQHQSSPLSEFEIEHYVEEVGEQRFFDSKSRSTAAPLSYKDKDVTVIFRRRGGD 240

[Close]

BLAST of CmaCh03G013510 vs. ExPASy TrEMBL
Match: A0A6J1CMV2 (MACPF domain-containing protein CAD1 OS=Momordica charantia OX=3673 GN=LOC111012420 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 3.6e-60
Identity = 147/319 (46.08%), Postives = 164/319 (51.41%), Query Frame = 0


Query: 45  MDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPNV 104
           MDALT TL  SI+ALGRGFDVTADIRLLYCKGTPGSRL+ L+D HTRDLVL+DG +VPNV
Sbjct: 21  MDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQLDDAHTRDLVLTDGVMVPNV 80

Query: 105 PDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAAT 164
           PDD+QCSS++R TEN+PVC+FHK+                          GS QVDAAAT
Sbjct: 81  PDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAAT 140

Query: 165 KSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKSI---------------- 224
           KSL M+GY IPLFKV LK+SNLVL EDIKRAVPYTWDPV+L S                 
Sbjct: 141 KSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGG 200

Query: 225 -------------PSPSTLLHTIK-------FSSNKLHLVGFIILKD------------- 243
                         S S + H +K         S     VG +  KD             
Sbjct: 201 RDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRRRGGD 260

[Close]

BLAST of CmaCh03G013510 vs. ExPASy TrEMBL
Match: A0A5A7U9L9 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002000 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 1.1e-59
Identity = 155/347 (44.67%), Postives = 168/347 (48.41%), Query Frame = 0


Query: 44  HMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPN 103
           +MDALTTTLC SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D HTRDLVLSDG VVPN
Sbjct: 18  NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPN 77

Query: 104 VPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAA 163
           VPDDVQCSS++R TEN+PVCSFHK+                          GS Q+DAAA
Sbjct: 78  VPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQLDAAA 137

Query: 164 TKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKSI--------------- 223
           TKSL MIGY+IP FKVALKSSNLVL EDIKRAVPYTWDPV+L S                
Sbjct: 138 TKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 197

Query: 224 -----------PSP-----------------------------------------STLLH 243
                       SP                                         S+L  
Sbjct: 198 GRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKVSRQQASSLFS 257

[Close]

BLAST of CmaCh03G013510 vs. NCBI nr
Match: XP_038883129.1 (MACPF domain-containing protein CAD1 [Benincasa hispida])

HSP 1 Score: 250.8 bits (639), Expect = 1.2e-62
Identity = 155/320 (48.44%), Postives = 170/320 (53.12%), Query Frame = 0


Query: 44  HMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPN 103
           +MDALTTTLC SI ALGRGFDVTADIRLLYCKGTPGSRLVHL+D  TRDLVLSDG VVPN
Sbjct: 17  NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVHLDDARTRDLVLSDGVVVPN 76

Query: 104 VPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAA 163
           VPDDVQCSS+SR TEN+PVCSFHK+                          GS QVDAAA
Sbjct: 77  VPDDVQCSSDSRATENVPVCSFHKMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVDAAA 136

Query: 164 TKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKS---------------- 223
           TKSL MIGY+IPLFKVALK+SNLVL EDIKRAVPYTWDPV+L S                
Sbjct: 137 TKSLAMIGYYIPLFKVALKNSNLVLCEDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 196

Query: 224 ------------------------------------------------------------ 243
                                                                       
Sbjct: 197 GRDVVYIRQHQSSPLSELEIEHYVKEVGEQRFLDSKSQSTASPLSYKDKDVTVIFRRRGG 256

[Close]

BLAST of CmaCh03G013510 vs. NCBI nr
Match: XP_008439705.1 (PREDICTED: MACPF domain-containing protein CAD1 [Cucumis melo] >TYK13232.1 MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 248.1 bits (632), Expect = 8.0e-62
Identity = 153/320 (47.81%), Postives = 169/320 (52.81%), Query Frame = 0


Query: 44  HMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPN 103
           +MDALTTTLC SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D HTRDLVLSDG VVPN
Sbjct: 18  NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPN 77

Query: 104 VPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAA 163
           VPDDVQCSS++R TEN+PVCSFHK+                          GS Q+DAAA
Sbjct: 78  VPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQLDAAA 137

Query: 164 TKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKS---------------- 223
           TKSL MIGY+IP FKVALKSSNLVL EDIKRAVPYTWDPV+L S                
Sbjct: 138 TKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 197

Query: 224 ------------------------------------------------------------ 243
                                                                       
Sbjct: 198 GRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKDVTVIFRRRGG 257

[Close]

BLAST of CmaCh03G013510 vs. NCBI nr
Match: XP_011658263.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KAE8647726.1 hypothetical protein Csa_003370 [Cucumis sativus])

HSP 1 Score: 247.3 bits (630), Expect = 1.4e-61
Identity = 155/323 (47.99%), Postives = 170/323 (52.63%), Query Frame = 0


Query: 41  PFLHMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPV 100
           P  +MDALTTTL  SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D H RDLVLSDG V
Sbjct: 15  PTSNMDALTTTLRNSIAALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHNRDLVLSDGVV 74

Query: 101 VPNVPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVD 160
           VPNVPDDVQCSS++R TEN+PVCSFHK+                          GS QVD
Sbjct: 75  VPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSDISGNIPLGSFNAMFNFSGSWQVD 134

Query: 161 AAATKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKS------------- 220
           AAATKSL MIGY+IPLFKVALKSSNLVL EDIKRAVPYTWDPV+L S             
Sbjct: 135 AAATKSLAMIGYYIPLFKVALKSSNLVLCEDIKRAVPYTWDPVSLASFIENFGTHIITSA 194

Query: 221 ------------------------------------------------------------ 243
                                                                       
Sbjct: 195 TIGGRDVVYIRQHQSSPLSEFEIEHYVEEVGEQRFFDSKSRSTAAPLSYKDKDVTVIFRR 254

[Close]

BLAST of CmaCh03G013510 vs. NCBI nr
Match: XP_022142258.1 (MACPF domain-containing protein CAD1 [Momordica charantia])

HSP 1 Score: 241.5 bits (615), Expect = 7.5e-60
Identity = 147/319 (46.08%), Postives = 164/319 (51.41%), Query Frame = 0


Query: 45  MDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPNV 104
           MDALT TL  SI+ALGRGFDVTADIRLLYCKGTPGSRL+ L+D HTRDLVL+DG +VPNV
Sbjct: 21  MDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQLDDAHTRDLVLTDGVMVPNV 80

Query: 105 PDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAAT 164
           PDD+QCSS++R TEN+PVC+FHK+                          GS QVDAAAT
Sbjct: 81  PDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAAT 140

Query: 165 KSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKSI---------------- 224
           KSL M+GY IPLFKV LK+SNLVL EDIKRAVPYTWDPV+L S                 
Sbjct: 141 KSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGG 200

Query: 225 -------------PSPSTLLHTIK-------FSSNKLHLVGFIILKD------------- 243
                         S S + H +K         S     VG +  KD             
Sbjct: 201 RDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRRRGGD 260

[Close]

BLAST of CmaCh03G013510 vs. NCBI nr
Match: KAA0052593.1 (MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 240.0 bits (611), Expect = 2.2e-59
Identity = 155/347 (44.67%), Postives = 168/347 (48.41%), Query Frame = 0


Query: 44  HMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPN 103
           +MDALTTTLC SI ALGRGFDVTADIRLLYCKGTPGSRLV L+D HTRDLVLSDG VVPN
Sbjct: 18  NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQLDDAHTRDLVLSDGVVVPN 77

Query: 104 VPDDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAA 163
           VPDDVQCSS++R TEN+PVCSFHK+                          GS Q+DAAA
Sbjct: 78  VPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPLGSFNAMFNFSGSWQLDAAA 137

Query: 164 TKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVALKSI--------------- 223
           TKSL MIGY+IP FKVALKSSNLVL EDIKRAVPYTWDPV+L S                
Sbjct: 138 TKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 197

Query: 224 -----------PSP-----------------------------------------STLLH 243
                       SP                                         S+L  
Sbjct: 198 GRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAAAPLSYKDKVSRQQASSLFS 257

[Close]

BLAST of CmaCh03G013510 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.8e-44
Identity = 114/318 (35.85%), Postives = 147/318 (46.23%), Query Frame = 0


Query: 46  DALTTTLCKSIEALGRGFDVTADIRLLYCKGTPGSRLVHLNDTHTRDLVLSDGPVVPNVP 105
           +ALTTTL  +I+ALGRGFDVT+D+RLLYCKG PGSRLV + +   RDL LS G ++PNVP
Sbjct: 15  EALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVP 74

Query: 106 DDVQCSSESRVTENLPVCSFHKVNSQL----------------------GSCQVDAAATK 165
            D+ CS  +  T+ + VCSFH++  +                       GS QVDAA+TK
Sbjct: 75  ADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTK 134

Query: 166 SLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL-------------------- 225
           SL ++GYFIPL+ V L    LVLH +I+RAVP +WDP +L                    
Sbjct: 135 SLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGR 194

Query: 226 --------KSIPSP---------------------STLLHTIKFSSNKL----------- 243
                   +S P P                      ++   +K+    +           
Sbjct: 195 DVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRRRGGDD 254

[Close]

BLAST of CmaCh03G013510 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 87.0 bits (214), Expect = 2.2e-17
Identity = 51/168 (30.36%), Postives = 81/168 (48.21%), Query Frame = 0


Query: 39  FLPFLHMDALTTTLCKSIEALGRGFDVTADIRLLYCKGTP-GSRLVHLNDTHTRDLVLSD 98
           F  F  +DA +    K++  +G G+D+ +D+R   CK TP GSRLV ++ T  RDL+   
Sbjct: 3   FNNFTRLDAHSAAE-KAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPG 62

Query: 99  GPVVPNVPDDVQCSSESRVTENLPVCSFHKVNSQLGSCQV-------------------- 158
           G VV NV   ++C    R      + SF++++ +                          
Sbjct: 63  GIVVNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCW 122

Query: 159 --DAAATKSLVMIGYFIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL 184
             DA++ K+L   G+FI L+ V +    L L +++KR VP +WD  AL
Sbjct: 123 PKDASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAAL 169

[Close]

BLAST of CmaCh03G013510 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 85.9 bits (211), Expect = 4.9e-17
Identity = 53/153 (34.64%), Postives = 78/153 (50.98%), Query Frame = 0


Query: 55  SIEALGRGFDVTADIRLLYCK-GTPGSRLVHLNDTHTR-DLVLSDGPVVPNVPDDVQCSS 114
           +I ++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G  +PNV   ++C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 115 ESRVTENLPVCSFHKVNSQ---------------------LGSC-QVDAAATKSLVMIGY 174
             R+     +  F ++  Q                       SC Q DAA TK+L   G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 175 FIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL 184
           FI L+ VAL  S ++L E +K+AVP TWDP AL
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAAL 166

[Close]

BLAST of CmaCh03G013510 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 85.9 bits (211), Expect = 4.9e-17
Identity = 53/153 (34.64%), Postives = 78/153 (50.98%), Query Frame = 0


Query: 55  SIEALGRGFDVTADIRLLYCK-GTPGSRLVHLNDTHTR-DLVLSDGPVVPNVPDDVQCSS 114
           +I ++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G  +PNV   ++C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 115 ESRVTENLPVCSFHKVNSQ---------------------LGSC-QVDAAATKSLVMIGY 174
             R+     +  F ++  Q                       SC Q DAA TK+L   G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 175 FIPLFKVALKSSNLVLHEDIKRAVPYTWDPVAL 184
           FI L+ VAL  S ++L E +K+AVP TWDP AL
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAAL 166

[Close]

BLAST of CmaCh03G013510 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 75.9 bits (185), Expect = 5.1e-14
Identity = 51/157 (32.48%), Postives = 74/157 (47.13%), Query Frame = 0


Query: 55  SIEALGRGFDVTADIRLLYCKGTPGS----RLVHLNDTHTRDLVLSDGPVVPNVPDDVQC 114
           ++++LG+GFD+TAD RL YCK   GS    RLV L+ T  R+L +    V  NV  D+ C
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 115 SSESRVTENLPVCSFHKVNS-----------------------QLGSCQVDAAATKSLVM 174
               R      +  F+K++                        Q GS   DAA  KSL +
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 175 IGYFIPLFKVALKSSN-LVLHEDIKRAVPYTWDPVAL 184
               + LF + + + N L L + ++ AVP +WDP  L
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLL 168

HSP 2 Score: 46.6 bits (109), Expect = 3.3e-05
Identity = 27/78 (34.62%), Postives = 38/78 (48.72%), Query Frame = 0


Query: 164 LVLHEDIKRAVPYTWDPVA--LKSIPSPSTLLHTIKFSSNKLHLVGFIILKDKPPIEDLQ 223
           L+   D   A+ + + P+   LK +P    L H +            + L+ KPP+ DLQ
Sbjct: 288 LITVPDKPDAINFNFIPITSLLKDVPGSGLLSHAMS-----------LYLRYKPPLMDLQ 347

Query: 224 YFLDFQIGRAWAPEQTNL 240
           YFLDF   RAWAP   +L
Sbjct: 348 YFLDFSGPRAWAPVHNDL 354

The following BLAST results are available for this feature:

BLAST of CmaCh03G013510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7N2	3.9e-43	35.85	MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=...	[more]
Q9SGN6	3.1e-16	30.36	MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=...	[more]
Q9STW5	6.9e-16	34.64	MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4...	[more]
Q8L612	7.1e-13	32.48	MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of CmaCh03G013510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CPE6	3.9e-62	47.81	MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3AZC7	3.9e-62	47.81	MACPF domain-containing protein CAD1 OS=Cucumis melo OX=3656 GN=LOC103484421 PE=...	[more]
A0A0A0KKH0	1.5e-61	48.28	MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G520370 PE=4 ...	[more]
A0A6J1CMV2	3.6e-60	46.08	MACPF domain-containing protein CAD1 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A5A7U9L9	1.1e-59	44.67	MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]

BLAST of CmaCh03G013510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883129.1	1.2e-62	48.44	MACPF domain-containing protein CAD1 [Benincasa hispida]	[more]
XP_008439705.1	8.0e-62	47.81	PREDICTED: MACPF domain-containing protein CAD1 [Cucumis melo] >TYK13232.1 MACPF...	[more]
XP_011658263.1	1.4e-61	47.99	MACPF domain-containing protein CAD1 [Cucumis sativus] >KAE8647726.1 hypothetica...	[more]
XP_022142258.1	7.5e-60	46.08	MACPF domain-containing protein CAD1 [Momordica charantia]	[more]
KAA0052593.1	2.2e-59	44.67	MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa]	[more]

BLAST of CmaCh03G013510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29690.1	2.8e-44	35.85	MAC/Perforin domain-containing protein	[more]
AT1G28380.1	2.2e-17	30.36	MAC/Perforin domain-containing protein	[more]
AT4G24290.1	4.9e-17	34.64	MAC/Perforin domain-containing protein	[more]
AT4G24290.2	4.9e-17	34.64	MAC/Perforin domain-containing protein	[more]
AT1G14780.1	5.1e-14	32.48	MAC/Perforin domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044663	MACPF domain-containing protein CAD1/NSL1-like	PANTHER	PTHR33199	MACPF DOMAIN-CONTAINING PROTEIN CAD1	coord: 132..185
IPR044663	MACPF domain-containing protein CAD1/NSL1-like	PANTHER	PTHR33199	MACPF DOMAIN-CONTAINING PROTEIN CAD1	coord: 210..242 coord: 45..129
None	No IPR available	PANTHER	PTHR33199:SF3	MACPF DOMAIN-CONTAINING PROTEIN CAD1	coord: 210..242 coord: 45..129
None	No IPR available	PANTHER	PTHR33199:SF3	MACPF DOMAIN-CONTAINING PROTEIN CAD1	coord: 132..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G013510.1	CmaCh03G013510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0012501	programmed cell death
biological_process	GO:2000031	regulation of salicylic acid mediated signaling pathway