CmaCh03G010150 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G010150
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Nuclear ribonuclease Z
Location	Cma_Chr03: 7074388 .. 7078813 (+)
RNA-Seq Expression	CmaCh03G010150
Synteny	CmaCh03G010150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGGTGGAACCCGAACAAAGAGAAGTTCAAAATATTGAAGCAGCAGAGCTTACCAGTCTCCGTCGTTTCTCTGCTCATAAATTTCATCCATGGAAGCCGGAACCGGAAGTAGCAGCAAAAGCCTTGACTCGGACCTGAAGGAAAGGATGAAGGGTATGCAAATTGAAGGATACCCAATTAAGGGTTTGTCCATTGGAGGCCATGAAACTTGCATCATTTTCCCAACCCTCAATTTGGCCTTTGATATTGGTCGCTGCCCCCAAAAGGCTATAAGCCAGAACTTCCTCCTCATCTCCCATGGCCATATGGATCACATTGTAAGTCCAACTCCCTCTCACCGCTTCATTTTTCAACTTTCTCATTGTCGTCTGAGCTTGTTCACATGGCGTGAGACCTCACGGTCACTACGCTTAGTAGATGCAATAACTTGTGCAGAAATGTCGAAGTGAAATTACATGTGGTTTAGGGCTTGAAGTCTAGTCATTACCTGTGGTTCTGCTGCAGCTATCTTCAATTCAAGTATCTATTTAAGATTTGAATCTAATTTTACCTTCCCGACGTTGTTGGGAAGATCTTATAAAATTCAAATCAGACATCGATCGCATTTCGAATGTAAGCTTTTTCGCGTATTGTTCATCTGCAGTTTACCTGTAGCTTTTGTGCTTAATAGGGTGGACTTCCCTTATACGTAGCTACGCGTGGATTATACAAAATGGAGCCTCCTACAATCATTGTACCCAAATCTATAAAGAATGATGTAGAAAATCTCTTAGAGGTGCACAGAAGATTGGATCAGTCTGAGCTGAGATGTAATTTGATAGGCTTGGAAGTGGGTAGTGCCCCTTATTGAATCTTCTTATTTTGTCCAATGAATAATTTGGTCACTTTTTGCCCAATTTTCGTGTTGCAGTGTAATACATTCTTCCATTCATATATATTGTTTGCAGGAGAAGAGTTTTCATTGAGAAGAGACCTCAAAGTGAGGGCTTTTAAGACATACCATGTCATTGACAGCCAGGTAACCTATTTAATGCACATATACTTTGATGATTTGGTTTTTTTTTTTTTTTTTGTCCAAAATTTACAGCCAACTCCTACACAAAAACTTTGGCAGGGCTATTTACTTTACTCTGTAAAACAGAAGCTTAAGAAAGAGTACTTGGGACTACCTGGCAACGAGATCAAGAAACTCCGGTTATCTGGTGTGGAGGTCTGTCCTTGACTGTCAAATTCATAGATTTCTTGGAACACATTCATTTTGAAAACTTCATTTTTATACACTCCGTCTATGCAAAAGCCTATAGTGGCCTCCCTGGCAGAACAATTTATCTAGAAATTTGTGAGATTTATCACTGGTGGGTGACCCGAAGAGTTTGAATCCACGATCATTTGGTTGAATTCAGGACTTTTCCACTATGTTTAAGCAATTTCTACTCATTTATAGTTTAACAAAGTTTGACGTTTTCCTTTGATTATGATTTTTCTTTCACGCGTTTCCCCATTCATTAAAATTCTCCTCAAATGCATAACTATCTTGGTTGTTTTTATTTGTGCCAGGTTACTTACACAATTACCGATCCTGAAGTGGCTTTTACAGGTGATACCACGTCCGACTTCATAGTTGATGAAAATAATATCGACGTATTGAGGGCAAAGATTCTAGTTATGGAGGTCTGGGACTTGAAGCCTGCACATTATATGACAATCTTATTTTCCTTTAACTTTTGTTCAAGTATTTTTATTTTAATTGAAATTTTAATACCATTGAAATTGATTTTGAATCATTAAAAACATGTTGTCGAGTGATTTTAAAGATTTTGAATGTGATAAGAGTGATTTTAACCATTTCAAATTAAACATGTTCATTGTATATTGGGCTTGGGGGTAGGTTGAGATTGGGAAGAAAAATGGTGTAGGTTATTATTTTGGGAGGGTGGGTTCTATTGGGGTTTAATCTCTCATGGTGCACAACCATTCTTCTTTGCAGAGCACATTCCTTGAAAATAGAGTTACAGTGGAGCATGCAAGAGAGTACGGACACACTCACCTGTTTGAGGTTTTCGTTCACTCAGCATTGTAATTCCTTCTATTGCTGTATTCATTCAGCAACTTATATCTTATATTTTTACATCCTAATGTGCTTTTTATGTTGATGATCCATCCATCCCAGATAATAAACCATGCCGAGAAGTTTAAGAACAAAGCGATTCTTATGATTCACTTTTCAGCTCGCTACACTACAGAGGTACTTGGATGCCAATTTATTGATATCTCTAGTTCATCATGCTTTTAGTTAGTTTACTGGTGCAATCCTTATATGAGGTGACATCATCTTCTTCCCACTTACATTTTTTAGAGAGGATTTCAGAGAATAACAAGTCTCAAGTTGCTCTTCTGGTTTGTTTTGGTACGTTTGGAAGGGTCTAAGCTAAATACATTTTCTGCTTTTTGGTTAATCAGGAAATTCAGCAAGCCGTAGCTGCTTTACCTCCACCTTTAGCAGGCCGAGTTTCTGCTCTAACGGAAGGTTTTTGAGAGAAAGGTATTTAGAAGCGTTTGGGAAATCATTTTGCCAATTTTTATGGTTGTTACAACAATCATTCAGTTCACGTATACTTCTTTTTTAGTTGTTTTATTGTGAATTTTCATGATTTGTTTCCATAAATGATTTATTTTTACTGTCAAAATGATGTTGATGCTTTCCAATTGGGCCATCTGTATTTTTGCAAGGGAAAATTCAAAATCAAACTCTTGTTATATTTGAGGATATTAATTGAATTAGCTCCTGCAGTTTTACGACTGCTAGTGAAGCTTAATGCGTTACTTAAATATTTTAAGTAGATACCATGTTATTCAACTTCATTATTTTAACAAGTAGAGGGCACAATTTGAATTCAAAACTAAAATGTTGAAAAATGGATGGCGTTGCTCAAAAAAAGAGAGAAAAAAAAGGACGTCAAGTCATGAGTTATGCTATAATTCACACACTATCTGCAAAATTATAACTTCAATTAAACCATATTAGGAACACAGAATACAAGACTTTACCCGATGCAAAACAATTTATCAAAACTGTGAATAGTTTCCTTGCAAATACCATTGAAGTACACCTCAATATTAAATCACAAGTCGTCCGTATCACCCAGAAGACCGATTAGCAGAAACCAGGAAAAAGAAAAAAAAAAAACTCGGCATTGGTAAACCGAAAGTGTTTTCACACGTTTTGTTTTTTCTTTTTTTACTCTCTAAGAAAAAGCAGTTTACAAAAACGTCCTCCAAATAGCCTTCAAAGTTGCTGTATAGTTGCGCTCAAATAAACCATGAACACATCTTACACCTCAGTTCTCAAATTGTCTGCTACATTAGCGACAACACAGCAAAGTTTCGATTCCAAGGCTAGAGAACTAACCAATTCCTCCATGGATTTCAGCTCCTCGTGAAAATCTGGATGCAATACCAATGAAGAAAAGTTCTCGTCCAGCATTAGTCCAAGATATCTGACGACATCATCAAGCTTGGGAACCCAATCACAAGCCTTCAAACCCAACACAGTAGCTGCTTTTGGACAATGAACTGCTTGAGGAAATCTCTCATATCTCTTTAACCATTTTCCCAGATATCGAATCAAACGAATCATCTCTTCATGGTTCAACTTATTGAGGGCAGAAGAAAGAATGACTTCATCGAGATTAGGTGATGCCAACAGATAATGCAAACACAGTTCCTGGGTTGAGAACCCATCATATGCTACCATGAGCTGAATTGCAGCCTCCTTTGCCAGATTGGACTTCTTGTCTTTGAGACTTTTATCACTCGCCTTCTCAATGGCTAGCAATGCCTGATCCTCCCATTCCTTCCTCACATTAGGCATACTGGCATAAGCCTCTTTGGAAGGGCAAAGAAAGTACTTCAAAATGCAATGTAAATCAGCAGGACCAAAATCTGATGCTTGCTTGATACAAAGACAAAGTAAGTCTGACCTCTTCTTCGCCACAAGTTTCAAAATCAAATTTGAATAAGATGCGTGTTTAACAAGTCTATTGACAACAAAAGTCTCCAGCAATTCCCATTCCTCTAAAGCAATACATGTATCCAAAACCAAGTCTCCAATATCTTTACTCATCAGGAACCCAAGTTTCTCAATCAGAATCTTAGTGTAACGATTGTCGGATGAACTAACACCAACCACAATTCCGACCGTTTCCCTCACTTTCTCAAGAAACACACATAAAATTTTTATAAACTCGGGCTTTGAAAAGTTTCGGTTTTTCAGCTTCAGCTTGAGATTTTCGTGGAATTTTTTCCCCAAATCAATCACCTTGGAATCGGTTTCAGATATTTTCCACCCTATAATGGGATTGACAAGAGAAACAGGATCTGGTTCATCTACTTTGGATTTCAAACTGGTTAGAATATCGTCTGGAT

mRNA sequence

GAAGGTGGAACCCGAACAAAGAGAAGTTCAAAATATTGAAGCAGCAGAGCTTACCAGTCTCCGTCGTTTCTCTGCTCATAAATTTCATCCATGGAAGCCGGAACCGGAAGTAGCAGCAAAAGCCTTGACTCGGACCTGAAGGAAAGGATGAAGGGTATGCAAATTGAAGGATACCCAATTAAGGGTTTGTCCATTGGAGGCCATGAAACTTGCATCATTTTCCCAACCCTCAATTTGGCCTTTGATATTGGTCGCTGCCCCCAAAAGGCTATAAGCCAGAACTTCCTCCTCATCTCCCATGGCCATATGGATCACATTGGTGGACTTCCCTTATACGTAGCTACGCGTGGATTATACAAAATGGAGCCTCCTACAATCATTGTACCCAAATCTATAAAGAATGATGTAGAAAATCTCTTAGAGGTGCACAGAAGATTGGATCAGTCTGAGCTGAGATGTAATTTGATAGGCTTGGAAGTGGGAGAAGAGTTTTCATTGAGAAGAGACCTCAAAGTGAGGGCTTTTAAGACATACCATGTCATTGACAGCCAGGGCTATTTACTTTACTCTGTAAAACAGAAGCTTAAGAAAGAGTACTTGGGACTACCTGGCAACGAGATCAAGAAACTCCGGTTATCTGGTGTGGAGGTTACTTACACAATTACCGATCCTGAAGTGGCTTTTACAGGTGATACCACGTCCGACTTCATAGTTGATGAAAATAATATCGACGTATTGAGGGCAAAGATTCTAGTTATGGAGAGCACATTCCTTGAAAATAGAGTTACAGTGGAGCATGCAAGAGAGTACGGACACACTCACCTGTTTGAGATAATAAACCATGCCGAGAAGTTTAAGAACAAAGCGATTCTTATGATTCACTTTTCAGCTCGCTACACTACAGAGGAAATTCAGCAAGCCGTAGCTGCTTTACCTCCACCTTTAGCAGGCCGAGTTTCTGCTCTAACGGAAGGTTTTTGAGAGAAAGCTCCTCGTGAAAATCTGGATGCAATACCAATGAAGAAAAGTTCTCGTCCAGCATTAGTCCAAGATATCTGACGACATCATCAAGCTTGGGAACCCAATCACAAGCCTTCAAACCCAACACAGTAGCTGCTTTTGGACAATGAACTGCTTGAGGAAATCTCTCATATCTCTTTAACCATTTTCCCAGATATCGAATCAAACGAATCATCTCTTCATGGTTCAACTTATTGAGGGCAGAAGAAAGAATGACTTCATCGAGATTAGGTGATGCCAACAGATAATGCAAACACAGTTCCTGGGTTGAGAACCCATCATATGCTACCATGAGCTGAATTGCAGCCTCCTTTGCCAGATTGGACTTCTTGTCTTTGAGACTTTTATCACTCGCCTTCTCAATGGCTAGCAATGCCTGATCCTCCCATTCCTTCCTCACATTAGGCATACTGGCATAAGCCTCTTTGGAAGGGCAAAGAAAGTACTTCAAAATGCAATGTAAATCAGCAGGACCAAAATCTGATGCTTGCTTGATACAAAGACAAAGTAAGTCTGACCTCTTCTTCGCCACAAGTTTCAAAATCAAATTTGAATAAGATGCGTGTTTAACAAGTCTATTGACAACAAAAGTCTCCAGCAATTCCCATTCCTCTAAAGCAATACATGTATCCAAAACCAAGTCTCCAATATCTTTACTCATCAGGAACCCAAGTTTCTCAATCAGAATCTTAGTGTAACGATTGTCGGATGAACTAACACCAACCACAATTCCGACCGTTTCCCTCACTTTCTCAAGAAACACACATAAAATTTTTATAAACTCGGGCTTTGAAAAGTTTCGGTTTTTCAGCTTCAGCTTGAGATTTTCGTGGAATTTTTTCCCCAAATCAATCACCTTGGAATCGGTTTCAGATATTTTCCACCCTATAATGGGATTGACAAGAGAAACAGGATCTGGTTCATCTACTTTGGATTTCAAACTGGTTAGAATATCGTCTGGAT

Coding sequence (CDS)

ATGGAAGCCGGAACCGGAAGTAGCAGCAAAAGCCTTGACTCGGACCTGAAGGAAAGGATGAAGGGTATGCAAATTGAAGGATACCCAATTAAGGGTTTGTCCATTGGAGGCCATGAAACTTGCATCATTTTCCCAACCCTCAATTTGGCCTTTGATATTGGTCGCTGCCCCCAAAAGGCTATAAGCCAGAACTTCCTCCTCATCTCCCATGGCCATATGGATCACATTGGTGGACTTCCCTTATACGTAGCTACGCGTGGATTATACAAAATGGAGCCTCCTACAATCATTGTACCCAAATCTATAAAGAATGATGTAGAAAATCTCTTAGAGGTGCACAGAAGATTGGATCAGTCTGAGCTGAGATGTAATTTGATAGGCTTGGAAGTGGGAGAAGAGTTTTCATTGAGAAGAGACCTCAAAGTGAGGGCTTTTAAGACATACCATGTCATTGACAGCCAGGGCTATTTACTTTACTCTGTAAAACAGAAGCTTAAGAAAGAGTACTTGGGACTACCTGGCAACGAGATCAAGAAACTCCGGTTATCTGGTGTGGAGGTTACTTACACAATTACCGATCCTGAAGTGGCTTTTACAGGTGATACCACGTCCGACTTCATAGTTGATGAAAATAATATCGACGTATTGAGGGCAAAGATTCTAGTTATGGAGAGCACATTCCTTGAAAATAGAGTTACAGTGGAGCATGCAAGAGAGTACGGACACACTCACCTGTTTGAGATAATAAACCATGCCGAGAAGTTTAAGAACAAAGCGATTCTTATGATTCACTTTTCAGCTCGCTACACTACAGAGGAAATTCAGCAAGCCGTAGCTGCTTTACCTCCACCTTTAGCAGGCCGAGTTTCTGCTCTAACGGAAGGTTTTTGA

Protein sequence

MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKAISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF

Homology

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BLAST of CmaCh03G010150 vs. ExPASy Swiss-Prot
Match: Q8LGU7 (tRNase Z TRZ1 OS=Arabidopsis thaliana OX=3702 GN=TRZ1 PE=1 SV=3)

HSP 1 Score: 441.8 bits (1135), Expect = 6.1e-123
Identity = 208/279 (74.55%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 18  ERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKAISQNFLLISHGHMDHIG 77
           E+ K MQIEGYPI+GLSIGGHETCIIFP+L +AFDIGRCP +AISQ+FL ISH HMDHIG
Sbjct: 2   EKKKAMQIEGYPIEGLSIGGHETCIIFPSLRIAFDIGRCPHRAISQDFLFISHSHMDHIG 61

Query: 78  GLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLR 137
           GLP+YVATRGLYKM+PPTIIVP SIK  VE+L EVHR+LD SEL+ NL+GL++GEEF +R
Sbjct: 62  GLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSELKHNLVGLDIGEEFIIR 121

Query: 138 RDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRLSGVEVTYTITDPEVA 197
           +DLKV+AFKT+HVI SQGY++YS K KLKKEY+GL GNEIK L++SGVE+T +I  PEVA
Sbjct: 122 KDLKVKAFKTFHVIQSQGYVVYSTKYKLKKEYIGLSGNEIKNLKVSGVEITDSIITPEVA 181

Query: 198 FTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAEKFKN 257
           FTGDTTSDF+VDE N D L+AK+LVMESTFL++ V+VEHAR+YGH H+ EI+NHAEKF+N
Sbjct: 182 FTGDTTSDFVVDETNADALKAKVLVMESTFLDDSVSVEHARDYGHIHISEIVNHAEKFEN 241

Query: 258 KAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           KAIL+IHFSARYT +EI+ AV+ALPPPL GRV ALT+GF
Sbjct: 242 KAILLIHFSARYTVKEIEDAVSALPPPLEGRVFALTQGF 280

[Close]

BLAST of CmaCh03G010150 vs. ExPASy Swiss-Prot
Match: Q8L633 (tRNase Z TRZ2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TRZ2 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.4e-90
Identity = 154/276 (55.80%), Postives = 208/276 (75.36%), Query Frame = 0


Query: 21  KGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKAISQNFLLISHGHMDHIGGLP 80
           KG+++E Y I+G+S+GGHETC+I P L   FDIGRCP +AI Q FL I+H H+DHIGGLP
Sbjct: 74  KGVELESYAIEGISVGGHETCVIVPELKCVFDIGRCPSRAIQQKFLFITHAHLDHIGGLP 133

Query: 81  LYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLRRDL 140
           +YVA+RGLY +EPP I VP SIK DVE LLE+HR + Q EL   LI L VGE + LR D+
Sbjct: 134 MYVASRGLYNLEPPKIFVPPSIKEDVEKLLEIHRTMGQVELNVELIPLAVGETYELRNDI 193

Query: 141 KVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRLSGVEVTYTITDPEVAFTG 200
            VR F T+HVI SQGY++YSV++KL+K+Y  L G +I+K++ SGVE+T TI  PE+AFTG
Sbjct: 194 VVRPFATHHVIPSQGYVIYSVRKKLQKQYAHLKGKQIEKIKKSGVEITDTILSPEIAFTG 253

Query: 201 DTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAEKFKNKAI 260
           DTTS++++D  N D LRAK+L+ E+TFL+   + EHA+  GHTH+ +II +A+  ++K +
Sbjct: 254 DTTSEYMLDPRNADALRAKVLITEATFLDESFSTEHAQALGHTHISQIIENAKWIRSKTV 313

Query: 261 LMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           L+ HFS+RY  EEI++AV  L   ++ +V  LTEGF
Sbjct: 314 LLTHFSSRYHVEEIREAVLKLQSKVSAKVIPLTEGF 349

[Close]

BLAST of CmaCh03G010150 vs. ExPASy Swiss-Prot
Match: P60193 (Nuclear ribonuclease Z (Fragment) OS=Triticum aestivum OX=4565 GN=ELAC PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 4.9e-72
Identity = 120/170 (70.59%), Postives = 152/170 (89.41%), Query Frame = 0


Query: 23  MQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKAISQNFLLISHGHMDHIGGLPLY 82
           ++IEGYP++G+SIGGHETC+IFPTL+LAFDIGRCPQ+A++Q+FL ISH H+DHIGGLP+Y
Sbjct: 7   LEIEGYPVEGISIGGHETCVIFPTLSLAFDIGRCPQRAVAQDFLFISHAHLDHIGGLPMY 66

Query: 83  VATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLRRDLKV 142
           VATRGLY++ PPTI VPK ++  VE L +VHR +DQSEL   L+ L++GEE+ LRRDLKV
Sbjct: 67  VATRGLYRLRPPTIFVPKYLRELVERLFDVHRAMDQSELNHTLVPLDIGEEYELRRDLKV 126

Query: 143 RAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRLSGVEVTYTIT 193
           RAFKTYH I SQGY++YSVKQKLK++YLGLPG+EIK+L+LSGVE+T T+T
Sbjct: 127 RAFKTYHTIPSQGYVIYSVKQKLKQDYLGLPGSEIKRLKLSGVEITNTVT 176

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BLAST of CmaCh03G010150 vs. ExPASy Swiss-Prot
Match: Q973F1 (Ribonuclease Z OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=rnz PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.8e-10
Identity = 60/221 (27.15%), Postives = 107/221 (48.42%), Query Frame = 0


Query: 68  ISHGHMDHIGGLPLYVATRGLYKMEPPTIIV-PKSIKNDVENLLEVHRRLDQSELRCNLI 127
           I+H H DH+ GLP  + T G+Y  +   +++ PK +K  +E++ +        E++    
Sbjct: 58  ITHMHGDHVLGLPGMIETMGMYSRKESLLLMGPKELKEFLEDIFKKTYFYPNFEIQI--- 117

Query: 128 GLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRL---- 187
            ++  E+     ++K+  F+T H I+SQGY L+  K +LK +   L    IK  R+    
Sbjct: 118 -IDKYED----ENIKISTFETCHTIESQGY-LFEEKDRLKIDIDKLRKEGIKDWRIIRML 177

Query: 188 ---SGVEVTYTITDPE----------VAFTGDTTSDFIVDENNIDVLR-AKILVMESTFL 247
                VE+   +  PE          +A+TGDT       E  I+ ++   +L+ +STF+
Sbjct: 178 KEGKRVEINGKVLLPEDYLIVKKGIRIAYTGDTGP----CEKVINAVKDVDLLIHDSTFI 237

Query: 248 ENRVTVEHAREYGHTHLFEIINHAEKFKNKAILMIHFSARY 270
           + +     A +YGH++ ++    A K   K + + H S RY
Sbjct: 238 DEK----EAYKYGHSNSYDAAYVALKANVKRLALFHISPRY 261

[Close]

BLAST of CmaCh03G010150 vs. ExPASy Swiss-Prot
Match: B0R7E2 (Ribonuclease Z OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) OX=478009 GN=rnz PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.7e-09
Identity = 68/257 (26.46%), Postives = 109/257 (42.41%), Query Frame = 0


Query: 51  FDIGRCPQKAISQ-------NFLLISHGHMDHIGGLPLYVATRGLY-KMEPPTIIVPKSI 110
           FD G   Q+ + +       + + I+HGH DH+ GLP  V T     + +P TI VP+ +
Sbjct: 35  FDAGEATQRQMMRYKTGFGVSDVFITHGHGDHVFGLPGLVHTWDFNDRTDPLTIHVPRGL 94

Query: 111 KNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVK 170
           + D+E+L  V            +     G       D +VRAF+T H   S GY L    
Sbjct: 95  RGDIEDL--VFSAGGDVGYPVRITEATPGAVVRSHDDYEVRAFETAHSTASVGYALVEDD 154

Query: 171 Q-----KLKKEYLGLP----------GNEIKKLRLSGVEVTYTITDPE----VAFTGDTT 230
           +     + + E LG+P          G  ++    + V     + DP     + +TGDT 
Sbjct: 155 RTGRFDRARAEELGVPVGPKFSTLHDGQPVELDDGTVVSPEQVVGDPRPGRTLVYTGDTR 214

Query: 231 SDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAEKFKNKAILMI 281
               V     D   A +L+ ++TF  +    E A E GH+   E  + A +   KA+ + 
Sbjct: 215 PHDPVVSAAED---ADLLIHDATFAND--ASERAAETGHSTAGEAADVATEAGAKALALT 274

[Close]

BLAST of CmaCh03G010150 vs. ExPASy TrEMBL
Match: A0A6J1IPL7 (tRNase Z TRZ1 OS=Cucurbita maxima OX=3661 GN=LOC111478792 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 8.7e-165
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. ExPASy TrEMBL
Match: A0A6J1GGV3 (tRNase Z TRZ1 OS=Cucurbita moschata OX=3662 GN=LOC111453818 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           MEAGTGSSSKSLDSDLKER KGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVA LPPPLAGRVSALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVATLPPPLAGRVSALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. ExPASy TrEMBL
Match: A0A6J1BWD5 (tRNase Z TRZ1 OS=Momordica charantia OX=3673 GN=LOC111005940 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.0e-150
Identity = 267/296 (90.20%), Postives = 278/296 (93.92%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           ME GTGSSSKS DSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQKA
Sbjct: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           I QNFL ISHGHMDHIGGLPLYVATRGLY MEPPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYNMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGL+VGEE SLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGL GNEIKKL
Sbjct: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTI++PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHAREY
Sbjct: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHL EIINHA+KFKNKA+L+IHFSARYTTEEIQQ +AALPPPLAGRV ALTEGF
Sbjct: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQTIAALPPPLAGRVFALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. ExPASy TrEMBL
Match: A0A5A7V6W7 (Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00700 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.3e-149
Identity = 267/297 (89.90%), Postives = 283/297 (95.29%), Query Frame = 0


Query: 1   MEAGTGS-SSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQK 60
           M AGT + SSK+LDS+  E  KG+QI GYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQS 120
           AISQ+FL ISHGHMDHIGGLPLYVATRGLYKM PPTI+VPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKK 180
           ELRCNLIGLEVGEE SLRRDLKVR F+TYHVIDSQGYLLYSVKQKLKK+YLGL GNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVE+TYTIT+PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           YGHTHLFEIINHA+KFKNKAIL+IHFSARYTTEEIQ+A++ LPPPLAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPPLAGRVSALTEGF 297

[Close]

BLAST of CmaCh03G010150 vs. ExPASy TrEMBL
Match: A0A1S3C5G3 (nuclear ribonuclease Z OS=Cucumis melo OX=3656 GN=LOC103497261 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.3e-148
Identity = 266/297 (89.56%), Postives = 282/297 (94.95%), Query Frame = 0


Query: 1   MEAGTGS-SSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQK 60
           M AGT + SSK+LDS+  E  KG+QI GYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQS 120
           AISQ+FL ISHGHMDHIGGLPLYVATRGLYKM PPTI+VPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKK 180
           ELRCNLIGLEVGEE SLRRDLKVR F+TYHVIDSQGYLLYSVKQKLKK+YLGL GNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVE+TYTIT+PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           YGHTHLFEIINHA+KFKNKAIL+IHFSARYTTEEIQ+A++ LPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPLLAGRVSALTEGF 297

[Close]

BLAST of CmaCh03G010150 vs. NCBI nr
Match: XP_022979026.1 (tRNase Z TRZ1 [Cucurbita maxima])

HSP 1 Score: 589.3 bits (1518), Expect = 1.8e-164
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. NCBI nr
Match: KAG6604210.1 (tRNase Z TRZ1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.0 bits (1512), Expect = 8.9e-164
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           MEAGTGSSSKSLDSDLKER KGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. NCBI nr
Match: XP_022950835.1 (tRNase Z TRZ1 [Cucurbita moschata])

HSP 1 Score: 585.5 bits (1508), Expect = 2.6e-163
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           MEAGTGSSSKSLDSDLKER KGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVA LPPPLAGRVSALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVATLPPPLAGRVSALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. NCBI nr
Match: XP_023543256.1 (tRNase Z TRZ1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 580.1 bits (1494), Expect = 1.1e-161
Identity = 291/296 (98.31%), Postives = 292/296 (98.65%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           MEAGTGSS KSLDSDLKER KGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSRKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQNFLLISHGHMDHIGGLPLYVATRGLYKM PPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMAPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEEFSL+RDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEY GLPGNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLKRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYFGLPGNEIKKL 180

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

[Close]

BLAST of CmaCh03G010150 vs. NCBI nr
Match: XP_038882012.1 (tRNase Z TRZ1 isoform X1 [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 1.9e-153
Identity = 274/296 (92.57%), Postives = 284/296 (95.95%), Query Frame = 0


Query: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60
           M AGTG SSKSLDSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTL+LAFDIGRCPQKA
Sbjct: 27  MAAGTGCSSKSLDSDPMESCKGMQIEGYPIKGLSIGGHETCVIFPTLSLAFDIGRCPQKA 86

Query: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120
           ISQ+FL ISHGHMDHIGGLPLYVATRGLYKM+PPTIIVPKSIKNDVENLLEVHRRLDQSE
Sbjct: 87  ISQDFLFISHGHMDHIGGLPLYVATRGLYKMKPPTIIVPKSIKNDVENLLEVHRRLDQSE 146

Query: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180
           LRCNLIGLEVGEE SLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGL GNEIKKL
Sbjct: 147 LRCNLIGLEVGEELSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLSGNEIKKL 206

Query: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240
           RLSG E+TYTIT+PEVAFTGDTTSDFIVDENNID+LRAKILVMESTFLENRVTVEHAREY
Sbjct: 207 RLSGAEITYTITEPEVAFTGDTTSDFIVDENNIDILRAKILVMESTFLENRVTVEHAREY 266

Query: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           GHTHLFEIINHAEKFKNKAIL+IHFSARYTTEEIQQA+A +PPPLAGRVSAL EGF
Sbjct: 267 GHTHLFEIINHAEKFKNKAILLIHFSARYTTEEIQQAIATIPPPLAGRVSALMEGF 322

[Close]

BLAST of CmaCh03G010150 vs. TAIR 10
Match: AT1G74700.1 (tRNAse Z1 )

HSP 1 Score: 441.8 bits (1135), Expect = 4.3e-124
Identity = 208/279 (74.55%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 18  ERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKAISQNFLLISHGHMDHIG 77
           E+ K MQIEGYPI+GLSIGGHETCIIFP+L +AFDIGRCP +AISQ+FL ISH HMDHIG
Sbjct: 2   EKKKAMQIEGYPIEGLSIGGHETCIIFPSLRIAFDIGRCPHRAISQDFLFISHSHMDHIG 61

Query: 78  GLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLR 137
           GLP+YVATRGLYKM+PPTIIVP SIK  VE+L EVHR+LD SEL+ NL+GL++GEEF +R
Sbjct: 62  GLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSELKHNLVGLDIGEEFIIR 121

Query: 138 RDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRLSGVEVTYTITDPEVA 197
           +DLKV+AFKT+HVI SQGY++YS K KLKKEY+GL GNEIK L++SGVE+T +I  PEVA
Sbjct: 122 KDLKVKAFKTFHVIQSQGYVVYSTKYKLKKEYIGLSGNEIKNLKVSGVEITDSIITPEVA 181

Query: 198 FTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAEKFKN 257
           FTGDTTSDF+VDE N D L+AK+LVMESTFL++ V+VEHAR+YGH H+ EI+NHAEKF+N
Sbjct: 182 FTGDTTSDFVVDETNADALKAKVLVMESTFLDDSVSVEHARDYGHIHISEIVNHAEKFEN 241

Query: 258 KAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           KAIL+IHFSARYT +EI+ AV+ALPPPL GRV ALT+GF
Sbjct: 242 KAILLIHFSARYTVKEIEDAVSALPPPLEGRVFALTQGF 280

[Close]

BLAST of CmaCh03G010150 vs. TAIR 10
Match: AT2G04530.1 (Metallo-hydrolase/oxidoreductase superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 9.7e-92
Identity = 154/276 (55.80%), Postives = 208/276 (75.36%), Query Frame = 0


Query: 21  KGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKAISQNFLLISHGHMDHIGGLP 80
           KG+++E Y I+G+S+GGHETC+I P L   FDIGRCP +AI Q FL I+H H+DHIGGLP
Sbjct: 74  KGVELESYAIEGISVGGHETCVIVPELKCVFDIGRCPSRAIQQKFLFITHAHLDHIGGLP 133

Query: 81  LYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEEFSLRRDL 140
           +YVA+RGLY +EPP I VP SIK DVE LLE+HR + Q EL   LI L VGE + LR D+
Sbjct: 134 MYVASRGLYNLEPPKIFVPPSIKEDVEKLLEIHRTMGQVELNVELIPLAVGETYELRNDI 193

Query: 141 KVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKLRLSGVEVTYTITDPEVAFTG 200
            VR F T+HVI SQGY++YSV++KL+K+Y  L G +I+K++ SGVE+T TI  PE+AFTG
Sbjct: 194 VVRPFATHHVIPSQGYVIYSVRKKLQKQYAHLKGKQIEKIKKSGVEITDTILSPEIAFTG 253

Query: 201 DTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAEKFKNKAI 260
           DTTS++++D  N D LRAK+L+ E+TFL+   + EHA+  GHTH+ +II +A+  ++K +
Sbjct: 254 DTTSEYMLDPRNADALRAKVLITEATFLDESFSTEHAQALGHTHISQIIENAKWIRSKTV 313

Query: 261 LMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 297
           L+ HFS+RY  EEI++AV  L   ++ +V  LTEGF
Sbjct: 314 LLTHFSSRYHVEEIREAVLKLQSKVSAKVIPLTEGF 349

The following BLAST results are available for this feature:

BLAST of CmaCh03G010150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LGU7	6.1e-123	74.55	tRNase Z TRZ1 OS=Arabidopsis thaliana OX=3702 GN=TRZ1 PE=1 SV=3	[more]
Q8L633	1.4e-90	55.80	tRNase Z TRZ2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TRZ2 PE=2 SV=1	[more]
P60193	4.9e-72	70.59	Nuclear ribonuclease Z (Fragment) OS=Triticum aestivum OX=4565 GN=ELAC PE=1 SV=1	[more]
Q973F1	1.8e-10	27.15	Ribonuclease Z OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 1...	[more]
B0R7E2	7.7e-09	26.46	Ribonuclease Z OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) OX=...	[more]

BLAST of CmaCh03G010150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IPL7	8.7e-165	100.00	tRNase Z TRZ1 OS=Cucurbita maxima OX=3661 GN=LOC111478792 PE=4 SV=1	[more]
A0A6J1GGV3	1.3e-163	99.32	tRNase Z TRZ1 OS=Cucurbita moschata OX=3662 GN=LOC111453818 PE=4 SV=1	[more]
A0A6J1BWD5	6.0e-150	90.20	tRNase Z TRZ1 OS=Momordica charantia OX=3673 GN=LOC111005940 PE=4 SV=1	[more]
A0A5A7V6W7	2.3e-149	89.90	Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8...	[more]
A0A1S3C5G3	3.3e-148	89.56	nuclear ribonuclease Z OS=Cucumis melo OX=3656 GN=LOC103497261 PE=4 SV=1	[more]

BLAST of CmaCh03G010150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022979026.1	1.8e-164	100.00	tRNase Z TRZ1 [Cucurbita maxima]	[more]
KAG6604210.1	8.9e-164	99.66	tRNase Z TRZ1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022950835.1	2.6e-163	99.32	tRNase Z TRZ1 [Cucurbita moschata]	[more]
XP_023543256.1	1.1e-161	98.31	tRNase Z TRZ1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_038882012.1	1.9e-153	92.57	tRNase Z TRZ1 isoform X1 [Benincasa hispida]	[more]

BLAST of CmaCh03G010150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74700.1	4.3e-124	74.55	tRNAse Z1	[more]
AT2G04530.1	9.7e-92	55.80	Metallo-hydrolase/oxidoreductase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001279	Metallo-beta-lactamase	PFAM	PF12706	Lactamase_B_2	coord: 65..265 e-value: 4.4E-10 score: 39.4
IPR036866	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like	GENE3D	3.60.15.10		coord: 29..283 e-value: 5.8E-45 score: 155.6
IPR036866	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like	SUPERFAMILY	56281	Metallo-hydrolase/oxidoreductase	coord: 43..277
None	No IPR available	PANTHER	PTHR46504	FAMILY NOT NAMED	coord: 17..296
None	No IPR available	CDD	cd16272	RNaseZ_MBL-fold	coord: 33..225 e-value: 1.10133E-27 score: 103.882

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G010150.1	CmaCh03G010150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034414	tRNA 3'-trailer cleavage, endonucleolytic
molecular_function	GO:0042781	3'-tRNA processing endoribonuclease activity