CmaCh03G008760 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G008760
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Cysteine desulfurase
Location	Cma_Chr03: 6469792 .. 6472934 (+)
RNA-Seq Expression	CmaCh03G008760
Synteny	CmaCh03G008760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCAAGATTCTTGCATCCAGCCTACGCCTCGCCCGCACAAATGTCTCTCCCGCCGTCTCCCGCTTCCGGCCTCTCTCCACCGCGGCTGCTGTAGCCACTTCTCCGTCGGAACCCCATAAGGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGATTTATCTTGATATGCAAGCTACATCCCCTGCTGATACTCGGGTGCTCGATGCTAGGCTTCCTTATTATCTCTCTCGATATGGTAATCCACCACTCTCAGACGCATCTCTATGGTTGGGAATCGGACCATGCCGTCGAGACGGCTTGCTCCCATGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTGACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGTCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCATTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGGTGGGTTTTGAGATTACCTACTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGATAAGCTCCGTTCTGCGATTAGACCCGACACTGGCCTTGTTTGTACTTTGTGCTTGATATGCAGATATAGAACAACCCCCTCCTCCTCCTCCTCCAGCAGGATTGTGATTAGAATAAACTCTGAATCTTACAAATGCATTCTTAAGAAGGGTAACGATTATTTAAGTTCTTAATCTTTGTCTCTTTACTTTTTCTTCCACCTGATTCATCAAAGGACAATTTAGCTAAATGCCTTTTATTTTCTTCTCCCTTCTTTCTGGTTGAATAGGTATGAATTTCTGGTTTCTCTTCCTTTTTTACCGATATGTAGCAGCTTACATTTCATTCACATGTTTTTTTTTGTAAGGCGCACAACTTTTTATTAAAGACGATGAGACATCCTCTCGTCTCACTCCCAGCTGACTAAGATTACAGAAATTTCTTAATCAAGAAGACAAGAATGGTTATGAAATCATCCTTTAGACAAAGGAATAGCCTTCAATTGGCTTAGATTTCATCGATATGATGTTCAGATTAATTTTAGACTCATGGTTCCACTTCTACCAATGCAAGATGAATGGGTGTGATGGTACACGCCTTAAGTTGATGAATAGTTTAGTCTCATGGAGCAACGTTTATTGGGGTAAAAGGCTAGTGAAAATCCTTATAGTTTTTAAGACCATGACACCTAGACAGTTATACAATTCTCATTCTTAACCTGATACGTCTGGACCGTGAAGTTTTTGTATGAACAGCAACTTTATGCAAGTAAAATGGCATGTGCAGTTTTGTCGCCATCTGCAGTGACACTCTGGATCAAGAAGACCCTGGCTATACCATTATTAATATTGCCTCTTCTAGATTAGGACTCTCTTTATTTATGTAAGCACTTGCTTCCATAAGGTTATTCTTTCATTCAATTTCAACTTCTTGCAGTTGCAGAGCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGTATGCTAGCCGTGCTGCAGGTAGACTGTATTAAGCCTTTACTATAGCTTTTTAGGATAAATCAGTTGAAAATTCTGTGCACGGTCATTGACAGCTATGATGATCTTACACGTTTGGATTTTATGCATGCTTCTCTGATTATAATGTTATAAAATTAACATTCTAAATCTTCCTATAAAAAACTAACATCCTAAATCCACGTCTTCTGCTGTTTAAAATGCAGTGAAACTTTCTAGGTACAAAACCAATAAGGAGATTCTAAAGAGAACTCTTTCAGTAGATCATTTTAAAAAAAAAAAAAAATTATTTATTTATTCATTTGTTGCAGTATTGTTGGTTGTCATGAATCCACACGTGTTAGGATATACATCTATTTCTACGAATATGTGTTCTAGAATGAACATCCCAAGTCCATGTCGTCTGATTTCCAAAATGAAGTGGAACTTTGTTGGACTTATTCCTTCTGGGTTTGCTATTGCCTGAAAGTTTGGTCATGAAAGGTGCAACCTTTTCTTGTTGGTCTGACTTTCTCATCAAGGAAAAGGTTCTTTGCCCTGTGGAACTTGAAACAGTGAAGCGTTCTATGTTCAAGGGGCTAGCCACTCTGCAGGAAGCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCACACAAAAAAAAAAAAGGAAGTGATCCTGGCTTCTAGGCGTGCTGTAGCGAATATGAACATGGGTGTGGTAAATTGAAATTTCAGATTTCCATGCGGATAAGAGCAAGAAGTGATTGAATGGCCATGTCTATGAAGTTCAAACTACTTGCCCAGTTGAGACTGTGTAGGAGGCCCAGGAGGCGCAGTTTAGTATCCTCTATTCTGTTTTGGACTTTCACTGTTTGGTTGTACACAAATGAGGACAATAAAATTAGTGAATGTTTTAGTGTTCCGCTAACAGTTTGCTAACACCAATACAGTTGTCTTGGTACTGCGTTCTTTTTTATGTTTATAAGTGAAGATGTCAACGAAACCTCTTTATTCATGAGCAGTGTACAACTTATTCTGTACAGCATCAAATTTATACCGACTTGGAACTTTATGCATCCTTGATACTATTTTATTTTCTTCTACAAAATGGAAGTTTTCATTTAGATATGAAAACTAGTACCTCTGATAGCAATATCATCACGAGCCAGCAGGACCCTGGAGACCTTGAAACAAGCTGTGAAAATCTTCTTGCTCCTGACAGAATCAATACATAATAGAAAGTTAGAAGAAACCAGTGGGTGGGGGTTTGAAATGGTGGGTGGGTGTGTGTGCTTGGTCTTTAATTGAGAGTGCATTATTTTTTGAAACATGCAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCTGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCTGTGCAGTGCAGTGGCCAAGTTTTGGGCAACCTGACTGAAGGCTTTCTTTTTTGTAAAATATGTGGAAGAAGAAGCCAAGGAAGATAGCAATATACGGTATCTGATTGGGAAAGTGACTCATTTCATTTATGAGATGAGAATCATGGCAATATCAGTTGTGGCCGTGGGGAGAAGGATTATGGGTATGGCCCAGCTGGGAAAGTGGGG

mRNA sequence

ATGGCGTCCAAGATTCTTGCATCCAGCCTACGCCTCGCCCGCACAAATGTCTCTCCCGCCGTCTCCCGCTTCCGGCCTCTCTCCACCGCGGCTGCTGTAGCCACTTCTCCGTCGGAACCCCATAAGGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGATTTATCTTGATATGCAAGCTACATCCCCTGCTGATACTCGGACGCATCTCTATGGTTGGGAATCGGACCATGCCGTCGAGACGGCTTGCTCCCATGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTGACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGTCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCATTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGGTGGGTTTTGAGATTACCTACTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGATAAGCTCCGTTCTGCGATTAGACCCGACACTGGCCTTGTTTGTACTTTGTGCTTGATATGCAGATATAGAACAACCCCCTCCTCCTCCTCCTCCAGCAGGATTGTGATTAGAATAAACTCTGAATCTTACAAATGCATTCTTAAGAAGGTTGCAGAGCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGTATGCTAGCCGTGCTGCAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCTGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCTGTGCAGTGCAGTGGCCAAGTTTTGGGCAACCTGACTGAAGGCTTTCTTTTTTGTAAAATATGTGGAAGAAGAAGCCAAGGAAGATAGCAATATACGGTATCTGATTGGGAAAGTGACTCATTTCATTTATGAGATGAGAATCATGGCAATATCAGTTGTGGCCGTGGGGAGAAGGATTATGGGTATGGCCCAGCTGGGAAAGTGGGG

Coding sequence (CDS)

ATGGCGTCCAAGATTCTTGCATCCAGCCTACGCCTCGCCCGCACAAATGTCTCTCCCGCCGTCTCCCGCTTCCGGCCTCTCTCCACCGCGGCTGCTGTAGCCACTTCTCCGTCGGAACCCCATAAGGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGATTTATCTTGATATGCAAGCTACATCCCCTGCTGATACTCGGACGCATCTCTATGGTTGGGAATCGGACCATGCCGTCGAGACGGCTTGCTCCCATGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTGACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGTCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCATTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGGTGGGTTTTGAGATTACCTACTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGATAAGCTCCGTTCTGCGATTAGACCCGACACTGGCCTTGTTTGTACTTTGTGCTTGATATGCAGATATAGAACAACCCCCTCCTCCTCCTCCTCCAGCAGGATTGTGATTAGAATAAACTCTGAATCTTACAAATGCATTCTTAAGAAGGTTGCAGAGCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGTATGCTAGCCGTGCTGCAGGTACTGTGTAGCAATATCCATTAA

Protein sequence

MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIYLDMQATSPADTRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRYRTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH

Homology

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BLAST of CmaCh03G008760 vs. ExPASy Swiss-Prot
Match: O49543 (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 9.0e-56
Identity = 115/205 (56.10%), Postives = 148/205 (72.20%), Query Frame = 0


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASK++++++R   T      SR R LSTAAA     +E + ++  I MKGV+ISGRP+Y
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQAT+P D                   +RTHLYGWE+++AVE A + VA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV  SGATE+NN+++KGVM+FY++ K+H+IT QTEHKCVLDSCRHLQQ GFE+TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DG+VDL+ LR AIRPDTGLV  + +
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAV 201

[Close]

BLAST of CmaCh03G008760 vs. ExPASy Swiss-Prot
Match: P0DN31 (Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_05732-2 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 5.7e-42
Identity = 95/191 (49.74%), Postives = 118/191 (61.78%), Query Frame = 0


Query: 13  ARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISG-RPIYLDMQATSPAD- 72
           A+T V  AV   +  S      T  +  +     +    +   G RPIYLDMQAT+P D 
Sbjct: 57  AQTTVESAVKTEQKASVPGTAVTGDALANPSAGILKQATIMDEGNRPIYLDMQATTPTDP 116

Query: 73  ------------------TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESN 132
                             +RTH YGWE++ A E A SHVA LIGA PKEI+ TSGATESN
Sbjct: 117 RVLDAMLPFLTGLYGNPHSRTHAYGWETEKATEQARSHVATLIGADPKEIIFTSGATESN 176

Query: 133 NISIKGVMNFYRE--KKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKL 182
           N+SIKGV  F+    KK+H+IT QTEHKCVLDSCRHLQ  GFE+TYLPV S+G++ ++ L
Sbjct: 177 NMSIKGVARFFGRSGKKKHIITTQTEHKCVLDSCRHLQDEGFEVTYLPVQSNGLIKIEDL 236

[Close]

BLAST of CmaCh03G008760 vs. ExPASy Swiss-Prot
Match: Q9VKD3 (Cysteine desulfurase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Nfs1 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.3e-41
Identity = 96/199 (48.24%), Postives = 121/199 (60.80%), Query Frame = 0


Query: 4   KILASSLRLARTNVSPAVSRFRPLS--TAAAVATSPSEPHKDETGISMKGVKISGRPIYL 63
           +I A  LR   TN    V R    S  TAA  A +  E  + +   ++K  +  GRP+YL
Sbjct: 7   RIQAQVLRSRATNALANVVRHEATSSRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYL 66

Query: 64  DMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKEI 123
           D QAT+P D                   +RTH YGWE++ AVE A   VA LIGA PKEI
Sbjct: 67  DAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGADPKEI 126

Query: 124 VLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSD 182
           + TSGATESNNI++KGV  FY  KKRH+IT QTEHKCVLDSCR L+  GF++TYLPV ++
Sbjct: 127 IFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLAN 186

[Close]

BLAST of CmaCh03G008760 vs. ExPASy Swiss-Prot
Match: Q9Y697 (Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3)

HSP 1 Score: 170.2 bits (430), Expect = 2.8e-41
Identity = 83/147 (56.46%), Postives = 104/147 (70.75%), Query Frame = 0


Query: 57  RPIYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGA 116
           RP+Y+D+QAT+P D                   +RTH YGWES+ A+E A   VA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYL 176
            P+EI+ TSGATESNNI+IKGV  FYR +K+HLIT QTEHKCVLDSCR L+  GF++TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLDKLRSAIRPDTGLVCTL 185
           PV   GI+DL +L +AI+PDT LV  +
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVM 203

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BLAST of CmaCh03G008760 vs. ExPASy Swiss-Prot
Match: Q5RDE7 (Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.8e-41
Identity = 83/147 (56.46%), Postives = 104/147 (70.75%), Query Frame = 0


Query: 57  RPIYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGA 116
           RP+Y+D+QAT+P D                   +RTH YGWES+ A+E A   VA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYL 176
            P+EI+ TSGATESNNI+IKGV  FYR +K+HLIT QTEHKCVLDSCR L+  GF++TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLDKLRSAIRPDTGLVCTL 185
           PV   GI+DL +L +AI+PDT LV  +
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVM 203

[Close]

BLAST of CmaCh03G008760 vs. ExPASy TrEMBL
Match: A0A6J1IQC5 (cysteine desulfurase, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477853 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.9e-93
Identity = 173/175 (98.86%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 71  TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 130
           ++THLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL
Sbjct: 16  SQTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 75

Query: 131 ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 190
           ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY
Sbjct: 76  ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 135

Query: 191 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH 246
           RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH
Sbjct: 136 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH 190

[Close]

BLAST of CmaCh03G008760 vs. ExPASy TrEMBL
Match: A0A6J1IIV0 (cysteine desulfurase, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477853 PE=3 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.9e-89
Identity = 167/169 (98.82%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 71  TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 130
           ++THLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL
Sbjct: 16  SQTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 75

Query: 131 ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 190
           ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY
Sbjct: 76  ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 135

Query: 191 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQV 240
           RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQV
Sbjct: 136 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQV 184

[Close]

BLAST of CmaCh03G008760 vs. ExPASy TrEMBL
Match: A0A6J1IRP7 (cysteine desulfurase, mitochondrial-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111477853 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 8.4e-89
Identity = 166/168 (98.81%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 71  TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 130
           ++THLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL
Sbjct: 16  SQTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 75

Query: 131 ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 190
           ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY
Sbjct: 76  ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 135

Query: 191 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQ 239
           RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQ
Sbjct: 136 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQ 183

[Close]

BLAST of CmaCh03G008760 vs. ExPASy TrEMBL
Match: A0A5D3CMX0 (Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003690 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.1e-79
Identity = 161/205 (78.54%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASKILAS LR   TN SP +SRFRP+STAAAVA SPSEPH+DETGISMKGVKISGRP+Y
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV TSGATESNNISIKGVM+FYREKKRHLIT QTEHKCVLDSCRHLQQ GFEITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DGIVDL+KLRSAIRPDTGLV  + +
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAV 205

[Close]

BLAST of CmaCh03G008760 vs. ExPASy TrEMBL
Match: A0A1S3B0Q9 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.1e-79
Identity = 161/205 (78.54%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASKILAS LR   TN SP +SRFRP+STAAAVA SPSEPH+DETGISMKGVKISGRP+Y
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV TSGATESNNISIKGVM+FYREKKRHLIT QTEHKCVLDSCRHLQQ GFEITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DGIVDL+KLRSAIRPDTGLV  + +
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAV 205

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: XP_022977558.1 (cysteine desulfurase, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 352.1 bits (902), Expect = 4.0e-93
Identity = 173/175 (98.86%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 71  TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 130
           ++THLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL
Sbjct: 16  SQTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 75

Query: 131 ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 190
           ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY
Sbjct: 76  ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 135

Query: 191 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH 246
           RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH
Sbjct: 136 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH 190

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: XP_022977557.1 (cysteine desulfurase, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 338.2 bits (866), Expect = 6.0e-89
Identity = 167/169 (98.82%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 71  TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 130
           ++THLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL
Sbjct: 16  SQTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 75

Query: 131 ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 190
           ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY
Sbjct: 76  ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 135

Query: 191 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQV 240
           RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQV
Sbjct: 136 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQV 184

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: XP_022977559.1 (cysteine desulfurase, mitochondrial-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 1.7e-88
Identity = 166/168 (98.81%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 71  TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 130
           ++THLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL
Sbjct: 16  SQTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHL 75

Query: 131 ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 190
           ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY
Sbjct: 76  ITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRY 135

Query: 191 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQ 239
           RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQ
Sbjct: 136 RTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQ 183

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: XP_008440458.1 (PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cysteine desulfurase [Cucumis melo var. makuwa] >TYK12780.1 cysteine desulfurase [Cucumis melo var. makuwa])

HSP 1 Score: 305.4 bits (781), Expect = 4.3e-79
Identity = 161/205 (78.54%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASKILAS LR   TN SP +SRFRP+STAAAVA SPSEPH+DETGISMKGVKISGRP+Y
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV TSGATESNNISIKGVM+FYREKKRHLIT QTEHKCVLDSCRHLQQ GFEITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DGIVDL+KLRSAIRPDTGLV  + +
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAV 205

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: XP_038882447.1 (cysteine desulfurase, mitochondrial [Benincasa hispida])

HSP 1 Score: 301.6 bits (771), Expect = 6.2e-78
Identity = 159/205 (77.56%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASK+LAS+LR   TN SPAVSRFRP+STAAAVA SPSEP +DETGISMKGVKISGRP+Y
Sbjct: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           I+ TSGATESNNISIKGVM+FYREKKRHLIT QTEHKCVLDSCRHLQQ GF+ITYLPVGS
Sbjct: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DGIVDLDKLRSAIR DTGLV  + +
Sbjct: 181 DGIVDLDKLRSAIRADTGLVSVMAV 205

[Close]

BLAST of CmaCh03G008760 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 218.4 bits (555), Expect = 6.4e-57
Identity = 115/205 (56.10%), Postives = 148/205 (72.20%), Query Frame = 0


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASK++++++R   T      SR R LSTAAA     +E + ++  I MKGV+ISGRP+Y
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQAT+P D                   +RTHLYGWE+++AVE A + VA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV  SGATE+NN+++KGVM+FY++ K+H+IT QTEHKCVLDSCRHLQQ GFE+TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DG+VDL+ LR AIRPDTGLV  + +
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAV 201

[Close]

BLAST of CmaCh03G008760 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 115.2 bits (287), Expect = 7.6e-26
Identity = 50/73 (68.49%), Postives = 64/73 (87.67%), Query Frame = 0


Query: 114 ISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSA 173
           +++KGVM+FY++ K+H+IT QTEHKCVLDSCRHLQQ GFE+TYLPV +DG+VDL+ LR A
Sbjct: 1   MAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREA 60

Query: 174 IRPDTGLVCTLCL 187
           IRPDTGLV  + +
Sbjct: 61  IRPDTGLVSIMAV 73

[Close]

BLAST of CmaCh03G008760 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 45.1 bits (105), Expect = 9.7e-05
Identity = 46/187 (24.60%), Postives = 75/187 (40.11%), Query Frame = 0


Query: 27  LSTAAAVATSPSEPHKDETGISM-----KGVKI------SGRPIYLDMQATSPADTRT-- 86
           LS  +A A S +    D   +S+     K  +I        + +YLD  ATS        
Sbjct: 31  LSVCSAAAASSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLD 90

Query: 87  -----------------HLYGWESDHAVETACSHVAALIGAS-PKEIVLTSGATES-NNI 146
                            H    ++    E A   VA  I AS  +EIV T  ATE+ N +
Sbjct: 91  ALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLV 150

Query: 147 SIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAI 182
           +    ++  +     ++T+   H C++      Q+ G  + ++ +  D + D++KLR  I
Sbjct: 151 AYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELI 210

The following BLAST results are available for this feature:

BLAST of CmaCh03G008760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49543	9.0e-56	56.10	Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=...	[more]
P0DN31	5.7e-42	49.74	Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-46...	[more]
Q9VKD3	1.3e-41	48.24	Cysteine desulfurase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Nfs1 P...	[more]
Q9Y697	2.8e-41	56.46	Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3	[more]
Q5RDE7	2.8e-41	56.46	Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1	[more]

BLAST of CmaCh03G008760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IQC5	1.9e-93	98.86	cysteine desulfurase, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1IIV0	2.9e-89	98.82	cysteine desulfurase, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1IRP7	8.4e-89	98.81	cysteine desulfurase, mitochondrial-like isoform X3 OS=Cucurbita maxima OX=3661 ...	[more]
A0A5D3CMX0	2.1e-79	78.54	Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A1S3B0Q9	2.1e-79	78.54	Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1	[more]

BLAST of CmaCh03G008760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977558.1	4.0e-93	98.86	cysteine desulfurase, mitochondrial-like isoform X2 [Cucurbita maxima]	[more]
XP_022977557.1	6.0e-89	98.82	cysteine desulfurase, mitochondrial-like isoform X1 [Cucurbita maxima]	[more]
XP_022977559.1	1.7e-88	98.81	cysteine desulfurase, mitochondrial-like isoform X3 [Cucurbita maxima]	[more]
XP_008440458.1	4.3e-79	78.54	PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cyst...	[more]
XP_038882447.1	6.2e-78	77.56	cysteine desulfurase, mitochondrial [Benincasa hispida]	[more]

BLAST of CmaCh03G008760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65720.1	6.4e-57	56.10	nitrogen fixation S (NIFS)-like 1	[more]
AT5G65720.2	7.6e-26	68.49	nitrogen fixation S (NIFS)-like 1	[more]
AT1G08490.1	9.7e-05	24.60	chloroplastic NIFS-like cysteine desulfurase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000192	Aminotransferase class V domain	PFAM	PF00266	Aminotran_5	coord: 72..182 e-value: 6.2E-20 score: 71.5
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 60..188 e-value: 1.5E-29 score: 104.7
IPR010240	Cysteine desulfurase IscS	PANTHER	PTHR11601:SF34	CYSTEINE DESULFURASE, MITOCHONDRIAL	coord: 51..182
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE FAMILY MEMBER	coord: 51..182
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 56..182

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G008760.1	CmaCh03G008760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044571	[2Fe-2S] cluster assembly
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0031071	cysteine desulfurase activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity