CmaCh03G007720 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G007720 vs. ExPASy Swiss-Prot
Match: F4JQH3 (Aminopeptidase P1 OS=Arabidopsis thaliana OX=3702 GN=APP1 PE=1 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 5.2e-274
Identity = 454/701 (64.76%), Postives = 549/701 (78.32%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           M++ LS+LR LMASH+PPLDALVVPSEDYHQSEYVSARDKRREFVSGF+GSAGLALIT+K
Sbjct: 1   MSEILSSLRSLMASHSPPLDALVVPSEDYHQSEYVSARDKRREFVSGFSGSAGLALITKK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           +A LWTDGRYFLQA+QQLSD+W LMR+GEDP  ++WM+DNLP +A +GVD WCVSVDTA 
Sbjct: 61  EARLWTDGRYFLQALQQLSDEWTLMRMGEDPLVEVWMSDNLPEEANIGVDSWCVSVDTAN 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           +W  +F+KK QKL+ T+T+LVDEVWK+RPP E++PV++HPLE+AGRSV  K + LRAKL 
Sbjct: 121 RWGKSFAKKNQKLITTTTDLVDEVWKSRPPSEMSPVVVHPLEFAGRSVSHKFEDLRAKLK 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           +E A GL++  LDEVAWLYNIRG+DV+YCPVVHAFAI+T +SAF YV+K+KVSDE   Y 
Sbjct: 181 QEGARGLVIAALDEVAWLYNIRGTDVAYCPVVHAFAILTTDSAFLYVDKKKVSDEANSYF 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQL--NMSSSSIDIAGSNGTKVESQSSGLIWADPAQCC 300
              G+EVR+Y+ VI+DV+ LAS++L  + +S ++    +   +++S     +W DPA CC
Sbjct: 241 NGLGVEVREYTDVISDVALLASDRLISSFASKTVQHEAAKDMEIDSDQPDRLWVDPASCC 300

Query: 301 YALYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETY 360
           YALYSKL++++VLLQ SP++L+KALKN VEL+G+KNAH+RDGAAVVQYLVWLD Q+QE Y
Sbjct: 301 YALYSKLDAEKVLLQPSPISLSKALKNPVELEGIKNAHVRDGAAVVQYLVWLDNQMQELY 360

Query: 361 GASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAII 420
           GASGYFLE +  +K KPS++ KLTEV+VSDKLE+ RASK+HFRGLSFPTISSVG NAA+I
Sbjct: 361 GASGYFLEAEASKK-KPSETSKLTEVTVSDKLESLRASKEHFRGLSFPTISSVGSNAAVI 420

Query: 421 HYGPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITL 480
           HY P+ E CAE+DP+ +YL DSGAQYLDGTTDITRTVHFG PSAHEK CYTAV KGH+ L
Sbjct: 421 HYSPEPEACAEMDPDKIYLCDSGAQYLDGTTDITRTVHFGKPSAHEKECYTAVFKGHVAL 480

Query: 481 GNARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVP 540
           GNARFP GTN                                                  
Sbjct: 481 GNARFPKGTN-------------------------------------------------- 540

Query: 541 LQASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPL 600
                    G  LDILAR PLWK GLDY HGTGHG+GS+L VHEGPH +SFRP ARNVPL
Sbjct: 541 ---------GYTLDILARAPLWKYGLDYRHGTGHGVGSYLCVHEGPHQVSFRPSARNVPL 600

Query: 601 QASMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINT 660
           QA+MTVTDEPGYYEDG FGIRLENVL+V +A+T+FNFGDKGYL FEHITWAPYQ KLI+ 
Sbjct: 601 QATMTVTDEPGYYEDGNFGIRLENVLVVNDAETEFNFGDKGYLQFEHITWAPYQVKLIDL 641

Query: 661 SLLTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPI 700
             LT EE+DWLN+YHSKC+DILAP+++++E  WL KATEP+
Sbjct: 661 DELTREEIDWLNTYHSKCKDILAPFMNQTEMEWLKKATEPV 641

BLAST of CmaCh03G007720 vs. ExPASy Swiss-Prot
Match: D1ZKF3 (Probable Xaa-Pro aminopeptidase P OS=Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) OX=771870 GN=AMPP PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 5.1e-144
Identity = 299/702 (42.59%), Postives = 399/702 (56.84%), Query Frame = 0

Query: 3   DTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQA 62
           D L+ALR LM   +  +D  VVPSED H SEY++  D RR F+SGF+GSAG A++T  +A
Sbjct: 7   DRLAALRSLMKERS--VDIYVVPSEDSHASEYITDCDARRTFISGFSGSAGTAVVTLDKA 66

Query: 63  LLWTDGRYFLQAIQQLSDQWKLMRIG--EDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 122
            L TDGRYF QA +QL + W L++ G  + P+   W AD       VG+DP  +S   A+
Sbjct: 67  ALATDGRYFNQASKQLDENWHLLKTGLQDVPTWQEWTADESAGGKTVGIDPTLISPAVAE 126

Query: 123 KWIHTFSKK-QQKLVQTSTNLVDEVW-KNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAK 182
           K      K     L   + NLVD VW ++RPP    PV +   +YAG+   +KL  LR +
Sbjct: 127 KLNGDIKKHGGSGLKAVTENLVDLVWGESRPPRPSEPVFLLGAKYAGKGAAEKLTDLRKE 186

Query: 183 LSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRL 242
           L ++KA   +V  LDE+AWL+N+RG+D++Y PV  ++AIVT +SA  YV++ K++DEV+ 
Sbjct: 187 LEKKKAAAFVVSMLDEIAWLFNLRGNDITYNPVFFSYAIVTKDSATLYVDESKLTDEVKQ 246

Query: 243 YMEKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCC 302
           Y+ +NG E++ Y+ +  D   LA    N + S+ +        V +++S   WA      
Sbjct: 247 YLAENGTEIKPYTDLFKDTEVLA----NAAKSTSESEKPTKYLVSNKAS---WA----LK 306

Query: 303 YALYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETY 362
            AL  + + DEV   +SP+  AKA+KN  EL+G++  HIRDGAA+++Y  WL+ QL    
Sbjct: 307 LALGGEKHVDEV---RSPIGDAKAIKNETELEGMRKCHIRDGAALIKYFAWLEDQL---- 366

Query: 363 GASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAII 422
                            +   KL EV  +D+LE FR+ +  F GLSF TISS GPN AII
Sbjct: 367 ----------------VNKKAKLNEVEAADQLEKFRSEQSDFVGLSFDTISSTGPNGAII 426

Query: 423 HYGPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITL 482
           HY P+   C+ +DP ++YL DSGAQ+ DGTTD+TRT+HFG P+A EK  YT VLKG+I L
Sbjct: 427 HYKPERGACSVIDPNAIYLCDSGAQFYDGTTDVTRTLHFGQPTAAEKKSYTLVLKGNIAL 486

Query: 483 GNARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVP 542
             A FP GT+G  LD LAR  LWK GLDY HGTGHG+GSFLNVHEGP             
Sbjct: 487 DTAVFPKGTSGFALDALARQFLWKYGLDYRHGTGHGVGSFLNVHEGP------------- 546

Query: 543 LQASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPL 602
                                             GIG+             R    +VPL
Sbjct: 547 ---------------------------------IGIGT-------------RKAYIDVPL 606

Query: 603 QASMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINT 662
                ++ EPGYYEDG +GIR+EN+ IV+   T+  FGDK YL FEHIT  PY RKLI+ 
Sbjct: 607 APGNVLSIEPGYYEDGNYGIRIENLAIVREVKTEHQFGDKPYLGFEHITMVPYCRKLIDE 613

Query: 663 SLLTSEELDWLNSYHSKCRDILAPYLDESEKA--WLNKATEP 699
           SLLT EE DWLN  + + R  +A Y D  +    WL + T P
Sbjct: 667 SLLTQEEKDWLNKSNEEIRKNMAGYFDGDQLTTDWLLRETSP 613

BLAST of CmaCh03G007720 vs. ExPASy Swiss-Prot
Match: Q8RY11 (Aminopeptidase P2 OS=Arabidopsis thaliana OX=3702 GN=APP2 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 5.1e-144
Identity = 285/708 (40.25%), Postives = 409/708 (57.77%), Query Frame = 0

Query: 3   DTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQA 62
           + LS++R L +     +DA ++PS+D HQSE+++    RR ++SGFTGSAG A++T+ +A
Sbjct: 81  EKLSSIRRLFSEPGVGIDAYIIPSQDAHQSEFIAECYARRAYISGFTGSAGTAVVTKDKA 140

Query: 63  LLWTDGRYFLQAIQQLSDQWKLMRIGED--PSEDLWMADNLPADAAVGVDPWCVSVDTAQ 122
            LWTDGRYFLQA +QL+  W LMR G    P+   W+AD L     VG+DP+  S D A+
Sbjct: 141 ALWTDGRYFLQAEKQLNSSWILMRAGNPGVPTASEWIADVLAPGGRVGIDPFLFSADAAE 200

Query: 123 KWIHTFSKKQQKLVQT-STNLVDEVWK-NRPPPEINPVIIHPLEYAGRSVEDKLKTLRAK 182
           +     +KK  +LV   + NLVDE+WK +RP P    + IH L+YAG  V  KL +LR +
Sbjct: 201 ELKEVIAKKNHELVYLYNVNLVDEIWKDSRPKPPSRQIRIHDLKYAGLDVASKLLSLRNQ 260

Query: 183 LSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRL 242
           +       +++  LDE+AW+ N+RGSDV + PV++A+ IV ++ A  +V+  KV+ EV+ 
Sbjct: 261 IMDAGTSAIVISMLDEIAWVLNLRGSDVPHSPVMYAYLIVEVDQAQLFVDNSKVTVEVKD 320

Query: 243 YMEKNGIEVRDYSAVITDVSSLASN--QLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQ 302
           +++  GIE+R Y +++  + SLA+   QL M  S++++A  +  K   +     +   A+
Sbjct: 321 HLKNAGIELRPYDSILQGIDSLAARGAQLLMDPSTLNVAIISTYKSACERYSRNFESEAK 380

Query: 303 CCYALYSKLN----SDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDK 362
                    +    +   +  QSP++ AKA+KN  EL G+KN+H+RD AA+  +  WL++
Sbjct: 381 VKTKFTDSSSGYTANPSGIYMQSPISWAKAIKNDAELKGMKNSHLRDAAALAHFWAWLEE 440

Query: 363 QLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVG 422
           ++ +                     +  LTEV V+D+L  FR+ +  F   SF TIS  G
Sbjct: 441 EVHK---------------------NANLTEVDVADRLLEFRSMQDGFMDTSFDTISGSG 500

Query: 423 PNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVL 482
            N AIIHY P+ E+C+ +DP+ ++L DSGAQY+DGTTDITRTVHF  PSA EK C+T VL
Sbjct: 501 ANGAIIHYKPEPESCSRVDPQKLFLLDSGAQYVDGTTDITRTVHFSEPSAREKECFTRVL 560

Query: 483 KGHITLGNARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRP 542
           +GHI L  A FP GT G  LD  AR  LWK GLDY HGTGHG+G+ LNVHEGP  ISFR 
Sbjct: 561 QGHIALDQAVFPEGTPGFVLDGFARSSLWKIGLDYRHGTGHGVGAALNVHEGPQSISFR- 620

Query: 543 RARNVPLQASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPR 602
                    +MT                                                
Sbjct: 621 -------YGNMT------------------------------------------------ 680

Query: 603 ARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQ 662
               PLQ  M V++EPGYYED AFGIR+EN+L V++A+T   FG   YL FE +T+ P Q
Sbjct: 681 ----PLQNGMIVSNEPGYYEDHAFGIRIENLLHVRDAETPNRFGGATYLGFEKLTFFPIQ 707

Query: 663 RKLINTSLLTSEELDWLNSYHSKCRDILAPYLDES-EKAWLNKATEPI 700
            K+++ SLL+  E+DWLNSYH++  + ++P L+ S  + WL   T P+
Sbjct: 741 TKMVDVSLLSDTEVDWLNSYHAEVWEKVSPLLEGSTTQQWLWNNTRPL 707

BLAST of CmaCh03G007720 vs. ExPASy Swiss-Prot
Match: C5GXZ9 (Probable Xaa-Pro aminopeptidase P OS=Ajellomyces dermatitidis (strain ER-3 / ATCC MYA-2586) OX=559297 GN=AMPP PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.9e-143
Identity = 283/700 (40.43%), Postives = 393/700 (56.14%), Query Frame = 0

Query: 5   LSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQALL 64
           L+ LR LM      +D  +VPSED HQSEY++  D RREF+SGFTGSAG A+++  +A L
Sbjct: 10  LARLRELMQERK--VDVYIVPSEDSHQSEYIAPCDGRREFISGFTGSAGCAIVSMSKAAL 69

Query: 65  WTDGRYFLQAIQQLSDQWKLMRIGED--PSEDLWMADNLPADAAVGVDPWCVSVDTAQKW 124
            TDGRYF QA +QL + W L++ G +  P+   W A+       VGVDP  ++   A+  
Sbjct: 70  STDGRYFNQAAKQLDNNWMLLKRGFENMPTWQEWTAEQAEGGKVVGVDPSLITASEARSL 129

Query: 125 IHTFSKKQQKLVQTSTNLVDEVW-KNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLSR 184
             T  K    L     NL+D VW K RP      V +HP+E+AG+S E+K+  LR +L +
Sbjct: 130 SETIEKSGGSLQGVQENLIDLVWGKERPARPSEKVALHPIEFAGKSFEEKISDLRKELQK 189

Query: 185 EKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYME 244
           +K+ G ++  LDE+AWL+N+RG+D+ Y PV  A+AI+T  +A  Y++  K+  EV+ Y+ 
Sbjct: 190 KKSAGFVISMLDEIAWLFNLRGNDIPYNPVFFAYAIITPTTADLYIDDEKLPAEVKKYL- 249

Query: 245 KNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYAL 304
            + + V+ Y ++  D  +L+ +    S      + S    + +++S   W+       AL
Sbjct: 250 GDQVSVKPYGSIFEDAKALSQSAQKKSDGDASTSPSEKFLISTKAS---WS----LSLAL 309

Query: 305 YSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGAS 364
             + N +EV   +SP+  AKA+KN  EL+G++  HIRDGAA+ +Y  WL+ +L       
Sbjct: 310 GGEKNVEEV---RSPITDAKAIKNEAELEGMRACHIRDGAALTEYFAWLENEL------- 369

Query: 365 GYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHYG 424
                         +    L EV  SDKLE  R+  KHF GLSF TISS GPNAA+IHY 
Sbjct: 370 -------------VNKKTVLNEVDGSDKLEQIRSKHKHFVGLSFDTISSTGPNAAVIHYK 429

Query: 425 PQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGNA 484
            + +TC+ +DP+++YL DSGAQYLDGTTD TRT+HFG P+  E+  YT VLKG I++  A
Sbjct: 430 AERDTCSIIDPKAVYLCDSGAQYLDGTTDTTRTLHFGEPTEMERKAYTLVLKGLISIDTA 489

Query: 485 RFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 544
            FP GT G  LD  AR  LWK+GLDYLHGTGHG+GS+LNVHEGP                
Sbjct: 490 VFPKGTTGFALDAFARQHLWKEGLDYLHGTGHGVGSYLNVHEGP---------------- 549

Query: 545 SMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQAS 604
                                          G+G+             R +   V +   
Sbjct: 550 ------------------------------IGLGT-------------RVQYAEVAITPG 609

Query: 605 MTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSLL 664
             ++DEPG+YEDG FGIR+EN++I K   T   FG+K +L FEH+T  P  +KLIN SLL
Sbjct: 610 NVISDEPGFYEDGVFGIRIENIIIAKEVKTTHGFGEKPWLGFEHVTMTPLCQKLINPSLL 617

Query: 665 TSEELDWLNSYHSKCRDILAPYL--DESEKAWLNKATEPI 700
           T  E  W+N YHSK  +  + Y   DE  + WL + T+PI
Sbjct: 670 TDGEKKWVNDYHSKVWEKTSSYFENDELTRNWLKRETQPI 617

BLAST of CmaCh03G007720 vs. ExPASy Swiss-Prot
Match: C5K105 (Probable Xaa-Pro aminopeptidase P OS=Blastomyces gilchristii (strain SLH14081) OX=559298 GN=AMPP PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.9e-143
Identity = 283/700 (40.43%), Postives = 393/700 (56.14%), Query Frame = 0

Query: 5   LSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQALL 64
           L+ LR LM      +D  +VPSED HQSEY++  D RREF+SGFTGSAG A+++  +A L
Sbjct: 10  LARLRELMQERK--VDVYIVPSEDSHQSEYIAPCDGRREFISGFTGSAGCAIVSMSKAAL 69

Query: 65  WTDGRYFLQAIQQLSDQWKLMRIGED--PSEDLWMADNLPADAAVGVDPWCVSVDTAQKW 124
            TDGRYF QA +QL + W L++ G +  P+   W A+       VGVDP  ++   A+  
Sbjct: 70  STDGRYFNQAAKQLDNNWMLLKRGFENMPTWQEWTAEQAEGGKVVGVDPSLITASEARSL 129

Query: 125 IHTFSKKQQKLVQTSTNLVDEVW-KNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLSR 184
             T  K    L     NL+D VW K RP      V +HP+E+AG+S E+K+  LR +L +
Sbjct: 130 SETIEKSGGSLQGVQENLIDLVWGKKRPARPSEKVALHPIEFAGKSFEEKISDLRKELQK 189

Query: 185 EKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYME 244
           +K+ G ++  LDE+AWL+N+RG+D+ Y PV  A+AI+T  +A  Y++  K+  EV+ Y+ 
Sbjct: 190 KKSAGFVISMLDEIAWLFNLRGNDIPYNPVFFAYAIITPTTADLYIDDEKLPAEVKKYL- 249

Query: 245 KNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYAL 304
            + + V+ Y ++  D  +L+ +    S      + S    + +++S   W+       AL
Sbjct: 250 GDQVSVKPYGSIFEDAKALSQSAQKKSDGDASTSPSEKFLISTKAS---WS----LSLAL 309

Query: 305 YSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGAS 364
             + N +EV   +SP+  AKA+KN  EL+G++  HIRDGAA+ +Y  WL+ +L       
Sbjct: 310 GGEKNVEEV---RSPITDAKAIKNEAELEGMRACHIRDGAALTEYFAWLENEL------- 369

Query: 365 GYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHYG 424
                         +    L EV  SDKLE  R+  KHF GLSF TISS GPNAA+IHY 
Sbjct: 370 -------------VNKKTVLNEVDGSDKLEQIRSKHKHFVGLSFDTISSTGPNAAVIHYK 429

Query: 425 PQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGNA 484
            + +TC+ +DP+++YL DSGAQYLDGTTD TRT+HFG P+  E+  YT VLKG I++  A
Sbjct: 430 AERDTCSIIDPKAVYLCDSGAQYLDGTTDTTRTLHFGEPTEMERKAYTLVLKGLISIDTA 489

Query: 485 RFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 544
            FP GT G  LD  AR  LWK+GLDYLHGTGHG+GS+LNVHEGP                
Sbjct: 490 VFPKGTTGFALDAFARQHLWKEGLDYLHGTGHGVGSYLNVHEGP---------------- 549

Query: 545 SMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQAS 604
                                          G+G+             R +   V +   
Sbjct: 550 ------------------------------IGLGT-------------RVQYAEVAITPG 609

Query: 605 MTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSLL 664
             ++DEPG+YEDG FGIR+EN++I K   T   FG+K +L FEH+T  P  +KLIN SLL
Sbjct: 610 NVISDEPGFYEDGVFGIRIENIIIAKEVKTTHGFGEKPWLGFEHVTMTPLCQKLINPSLL 617

Query: 665 TSEELDWLNSYHSKCRDILAPYL--DESEKAWLNKATEPI 700
           T  E  W+N YHSK  +  + Y   DE  + WL + T+PI
Sbjct: 670 TDGEKKWVNDYHSKVWEKTSSYFENDELTRNWLKRETQPI 617

BLAST of CmaCh03G007720 vs. ExPASy TrEMBL
Match: A0A6J1IQ36 (probable Xaa-Pro aminopeptidase P isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478420 PE=3 SV=1)

HSP 1 Score: 1276.2 bits (3301), Expect = 0.0e+00
Identity = 642/701 (91.58%), Postives = 642/701 (91.58%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK
Sbjct: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ
Sbjct: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS
Sbjct: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM
Sbjct: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300
           EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA
Sbjct: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300

Query: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360
           LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA
Sbjct: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360

Query: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420
           SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY
Sbjct: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420

Query: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480
           GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN
Sbjct: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480

Query: 481 ARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQ 540
           ARFPNGTN                                                    
Sbjct: 481 ARFPNGTN---------------------------------------------------- 540

Query: 541 ASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600
                  GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA
Sbjct: 541 -------GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600

Query: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 660
           SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL
Sbjct: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 642

Query: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA
Sbjct: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 642

BLAST of CmaCh03G007720 vs. ExPASy TrEMBL
Match: A0A6J1GE61 (probable Xaa-Pro aminopeptidase P isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453176 PE=3 SV=1)

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 634/701 (90.44%), Postives = 639/701 (91.16%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK
Sbjct: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ
Sbjct: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           KWIH FSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS
Sbjct: 121 KWIHAFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           +EKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM
Sbjct: 181 QEKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300
           EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA
Sbjct: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300

Query: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360
           LYSKLNSD+VLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA
Sbjct: 301 LYSKLNSDKVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360

Query: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420
           SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY
Sbjct: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420

Query: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480
           GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHI LGN
Sbjct: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHIALGN 480

Query: 481 ARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQ 540
           ARFPNGTN                                                    
Sbjct: 481 ARFPNGTN---------------------------------------------------- 540

Query: 541 ASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600
                  GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARN+PLQA
Sbjct: 541 -------GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNIPLQA 600

Query: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 660
           SMTVTDEPGYYEDGAFGIRLENVLIVK+ADTKFNFGDKGYLSFEHITWAPYQRKLI+TSL
Sbjct: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKDADTKFNFGDKGYLSFEHITWAPYQRKLIDTSL 642

Query: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           LTSEELDWLNSYHSKCRDILAPYLDESEKAWLN ATEPIPA
Sbjct: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNNATEPIPA 642

BLAST of CmaCh03G007720 vs. ExPASy TrEMBL
Match: A0A6J1IMN2 (probable Xaa-Pro aminopeptidase P isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478420 PE=3 SV=1)

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 611/670 (91.19%), Postives = 611/670 (91.19%), Query Frame = 0

Query: 32  SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP 91
           SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP
Sbjct: 2   SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP 61

Query: 92  SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP 151
           SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP
Sbjct: 62  SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP 121

Query: 152 EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV 211
           EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV
Sbjct: 122 EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV 181

Query: 212 VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS 271
           VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS
Sbjct: 182 VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS 241

Query: 272 IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG 331
           IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG
Sbjct: 242 IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG 301

Query: 332 LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE 391
           LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE
Sbjct: 302 LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE 361

Query: 392 AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI 451
           AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI
Sbjct: 362 AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI 421

Query: 452 TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTNGQQLDILARVPLWKDGLDYLHGT 511
           TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTN                       
Sbjct: 422 TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTN----------------------- 481

Query: 512 GHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDGQQLDILARVPLWKDGLDYLHGTG 571
                                               GQQLDILARVPLWKDGLDYLHGTG
Sbjct: 482 ------------------------------------GQQLDILARVPLWKDGLDYLHGTG 541

Query: 572 HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT 631
           HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT
Sbjct: 542 HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT 601

Query: 632 KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW 691
           KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW
Sbjct: 602 KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW 612

Query: 692 LNKATEPIPA 702
           LNKATEPIPA
Sbjct: 662 LNKATEPIPA 612

BLAST of CmaCh03G007720 vs. ExPASy TrEMBL
Match: A0A6J1GDE9 (probable Xaa-Pro aminopeptidase P isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453176 PE=3 SV=1)

HSP 1 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 603/670 (90.00%), Postives = 608/670 (90.75%), Query Frame = 0

Query: 32  SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP 91
           SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP
Sbjct: 2   SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP 61

Query: 92  SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP 151
           SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIH FSKKQQKLVQTSTNLVDEVWKNRPPP
Sbjct: 62  SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHAFSKKQQKLVQTSTNLVDEVWKNRPPP 121

Query: 152 EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV 211
           EINPVIIHPLEYAGRSVEDKLKTLRAKLS+EKAHGLIVVGLDEVAWLYNIRGSDVSYCPV
Sbjct: 122 EINPVIIHPLEYAGRSVEDKLKTLRAKLSQEKAHGLIVVGLDEVAWLYNIRGSDVSYCPV 181

Query: 212 VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS 271
           VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS
Sbjct: 182 VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS 241

Query: 272 IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG 331
           IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSD+VLLQQSPLALAKALKNSVELDG
Sbjct: 242 IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDKVLLQQSPLALAKALKNSVELDG 301

Query: 332 LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE 391
           LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE
Sbjct: 302 LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE 361

Query: 392 AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI 451
           AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI
Sbjct: 362 AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI 421

Query: 452 TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTNGQQLDILARVPLWKDGLDYLHGT 511
           TRTVHFGLPSAHEKACYTAVLKGHI LGNARFPNGTN                       
Sbjct: 422 TRTVHFGLPSAHEKACYTAVLKGHIALGNARFPNGTN----------------------- 481

Query: 512 GHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDGQQLDILARVPLWKDGLDYLHGTG 571
                                               GQQLDILARVPLWKDGLDYLHGTG
Sbjct: 482 ------------------------------------GQQLDILARVPLWKDGLDYLHGTG 541

Query: 572 HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT 631
           HGIGSFLNVHEGPHLISFRPRARN+PLQASMTVTDEPGYYEDGAFGIRLENVLIVK+ADT
Sbjct: 542 HGIGSFLNVHEGPHLISFRPRARNIPLQASMTVTDEPGYYEDGAFGIRLENVLIVKDADT 601

Query: 632 KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW 691
           KFNFGDKGYLSFEHITWAPYQRKLI+TSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW
Sbjct: 602 KFNFGDKGYLSFEHITWAPYQRKLIDTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW 612

Query: 692 LNKATEPIPA 702
           LN ATEPIPA
Sbjct: 662 LNNATEPIPA 612

BLAST of CmaCh03G007720 vs. ExPASy TrEMBL
Match: A0A5A7T4E1 (Putative Xaa-Pro aminopeptidase P OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00480 PE=3 SV=1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 594/716 (82.96%), Postives = 622/716 (86.87%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           MADTLSALR+LMASH+PPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGS GLAL+TQ+
Sbjct: 1   MADTLSALRILMASHSPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSTGLALVTQR 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           +ALLWTDGRYFLQAIQQLSDQWKLMR+GEDP  DLWMADNLPADAAVGVDPWCVSVDTAQ
Sbjct: 61  EALLWTDGRYFLQAIQQLSDQWKLMRMGEDPPVDLWMADNLPADAAVGVDPWCVSVDTAQ 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           +WIH FSKK+QKLVQT+TNLVDEVWKNRPPPEINPV+IHPLE+ GRSVEDKLKTLR KLS
Sbjct: 121 RWIHAFSKKEQKLVQTTTNLVDEVWKNRPPPEINPVMIHPLEFTGRSVEDKLKTLRTKLS 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           +EKAHGLIV GLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYV+KRKVSDEVRLYM
Sbjct: 181 QEKAHGLIVTGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVDKRKVSDEVRLYM 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSS---------------SSIDIAGSNGTKVESQ 300
           E+NGIEVRDYSAVITDVS LASNQLN+SS               SSIDIAGSNGTKVESQ
Sbjct: 241 EQNGIEVRDYSAVITDVSLLASNQLNLSSFVKGSEVRANVEVELSSIDIAGSNGTKVESQ 300

Query: 301 SSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQ 360
           SSGLIW DPAQCCYALYSKLNSD+VLLQQSPLAL KALKNSVELDGLK AHIRDGAAVVQ
Sbjct: 301 SSGLIWVDPAQCCYALYSKLNSDKVLLQQSPLALEKALKNSVELDGLKKAHIRDGAAVVQ 360

Query: 361 YLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSF 420
           YLVWLDKQLQETYGASGYFLEGDGVRKPKPSDS+KLTEVSVSDKLEAFRASK+HFRGLSF
Sbjct: 361 YLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSKKLTEVSVSDKLEAFRASKEHFRGLSF 420

Query: 421 PTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEK 480
           PTISSVG NAAIIHYG +AETCAELDPES+YLFDSGAQYLDGTTDITRTVHFGLPSAHEK
Sbjct: 421 PTISSVGSNAAIIHYGAKAETCAELDPESIYLFDSGAQYLDGTTDITRTVHFGLPSAHEK 480

Query: 481 ACYTAVLKGHITLGNARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGP 540
           ACYTAVLKGHI LGNARFPNGTN                                     
Sbjct: 481 ACYTAVLKGHIALGNARFPNGTN------------------------------------- 540

Query: 541 HLISFRPRARNVPLQASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPH 600
                                 G  LDILARVPLWKDGLDY HGTGHGIGSFLNVHEGPH
Sbjct: 541 ----------------------GHSLDILARVPLWKDGLDYRHGTGHGIGSFLNVHEGPH 600

Query: 601 LISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEH 660
           LISFRP+ARNVPLQASMTVTDEPGYYEDGAFGIRLENVL+VK+A+TKFNFGDKGYLSFEH
Sbjct: 601 LISFRPQARNVPLQASMTVTDEPGYYEDGAFGIRLENVLVVKDANTKFNFGDKGYLSFEH 657

Query: 661 ITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           ITWAPYQRKLINTSLLT EEL+W+N+YHS+CRDILAPYLDESEK WLNKATEPI A
Sbjct: 661 ITWAPYQRKLINTSLLTFEELNWVNTYHSQCRDILAPYLDESEKLWLNKATEPITA 657

BLAST of CmaCh03G007720 vs. NCBI nr
Match: XP_022978435.1 (probable Xaa-Pro aminopeptidase P isoform X1 [Cucurbita maxima])

HSP 1 Score: 1276.2 bits (3301), Expect = 0.0e+00
Identity = 642/701 (91.58%), Postives = 642/701 (91.58%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK
Sbjct: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ
Sbjct: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS
Sbjct: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM
Sbjct: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300
           EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA
Sbjct: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300

Query: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360
           LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA
Sbjct: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360

Query: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420
           SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY
Sbjct: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420

Query: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480
           GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN
Sbjct: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480

Query: 481 ARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQ 540
           ARFPNGTN                                                    
Sbjct: 481 ARFPNGTN---------------------------------------------------- 540

Query: 541 ASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600
                  GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA
Sbjct: 541 -------GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600

Query: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 660
           SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL
Sbjct: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 642

Query: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA
Sbjct: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 642

BLAST of CmaCh03G007720 vs. NCBI nr
Match: XP_022949930.1 (probable Xaa-Pro aminopeptidase P isoform X1 [Cucurbita moschata])

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 634/701 (90.44%), Postives = 639/701 (91.16%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK
Sbjct: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ
Sbjct: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           KWIH FSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS
Sbjct: 121 KWIHAFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           +EKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM
Sbjct: 181 QEKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300
           EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA
Sbjct: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300

Query: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360
           LYSKLNSD+VLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA
Sbjct: 301 LYSKLNSDKVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360

Query: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420
           SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY
Sbjct: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420

Query: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480
           GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHI LGN
Sbjct: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHIALGN 480

Query: 481 ARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQ 540
           ARFPNGTN                                                    
Sbjct: 481 ARFPNGTN---------------------------------------------------- 540

Query: 541 ASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600
                  GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARN+PLQA
Sbjct: 541 -------GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNIPLQA 600

Query: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 660
           SMTVTDEPGYYEDGAFGIRLENVLIVK+ADTKFNFGDKGYLSFEHITWAPYQRKLI+TSL
Sbjct: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKDADTKFNFGDKGYLSFEHITWAPYQRKLIDTSL 642

Query: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           LTSEELDWLNSYHSKCRDILAPYLDESEKAWLN ATEPIPA
Sbjct: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNNATEPIPA 642

BLAST of CmaCh03G007720 vs. NCBI nr
Match: XP_023544152.1 (probable Xaa-Pro aminopeptidase P isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 635/701 (90.58%), Postives = 638/701 (91.01%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK
Sbjct: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ
Sbjct: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           KWIH FSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS
Sbjct: 121 KWIHAFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           +EKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTL SAFFYVEKRKVSDEVRLYM
Sbjct: 181 QEKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLISAFFYVEKRKVSDEVRLYM 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300
           EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA
Sbjct: 241 EKNGIEVRDYSAVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYA 300

Query: 301 LYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360
           LYSKLNSD+VLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA
Sbjct: 301 LYSKLNSDKVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGA 360

Query: 361 SGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420
           SGYFLEG GVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY
Sbjct: 361 SGYFLEGHGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHY 420

Query: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGN 480
           GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHI LGN
Sbjct: 421 GPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHIALGN 480

Query: 481 ARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQ 540
           ARFPNGTN                                                    
Sbjct: 481 ARFPNGTN---------------------------------------------------- 540

Query: 541 ASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600
                  GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA
Sbjct: 541 -------GQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQA 600

Query: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 660
           SMTVTDEPGYYEDGAFGIRLENVLIVK+ADTKFNFGDKGYLSFEHITWAPYQRKLINTSL
Sbjct: 601 SMTVTDEPGYYEDGAFGIRLENVLIVKDADTKFNFGDKGYLSFEHITWAPYQRKLINTSL 642

Query: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA
Sbjct: 661 LTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPIPA 642

BLAST of CmaCh03G007720 vs. NCBI nr
Match: KAG6603996.1 (Aminopeptidase P1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 626/690 (90.72%), Postives = 629/690 (91.16%), Query Frame = 0

Query: 12  MASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYF 71
           MASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYF
Sbjct: 1   MASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYF 60

Query: 72  LQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQ 131
           LQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQKWIH FSKKQQ
Sbjct: 61  LQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHAFSKKQQ 120

Query: 132 KLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVG 191
           KLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS+EKAHGLIVVG
Sbjct: 121 KLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLSQEKAHGLIVVG 180

Query: 192 LDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYS 251
           LDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYS
Sbjct: 181 LDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYS 240

Query: 252 AVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVL 311
           AVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSD+VL
Sbjct: 241 AVITDVSSLASNQLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDKVL 300

Query: 312 LQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVR 371
           LQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVR
Sbjct: 301 LQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVR 360

Query: 372 KPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELD 431
           KPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELD
Sbjct: 361 KPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELD 420

Query: 432 PESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTNGQQ 491
           PESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHI LGNARFPNGTN   
Sbjct: 421 PESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHIALGNARFPNGTN--- 480

Query: 492 LDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDGQQL 551
                                                                   GQQL
Sbjct: 481 --------------------------------------------------------GQQL 540

Query: 552 DILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYY 611
           DILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYY
Sbjct: 541 DILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYY 600

Query: 612 EDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNS 671
           EDGAFGIRLENVLIVK+ADTKFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNS
Sbjct: 601 EDGAFGIRLENVLIVKDADTKFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNS 631

Query: 672 YHSKCRDILAPYLDESEKAWLNKATEPIPA 702
           YHSKCRDILAPYLDESEKAWLNKATEPIPA
Sbjct: 661 YHSKCRDILAPYLDESEKAWLNKATEPIPA 631

BLAST of CmaCh03G007720 vs. NCBI nr
Match: XP_022978436.1 (probable Xaa-Pro aminopeptidase P isoform X2 [Cucurbita maxima])

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 611/670 (91.19%), Postives = 611/670 (91.19%), Query Frame = 0

Query: 32  SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP 91
           SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP
Sbjct: 2   SEYVSARDKRREFVSGFTGSAGLALITQKQALLWTDGRYFLQAIQQLSDQWKLMRIGEDP 61

Query: 92  SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP 151
           SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP
Sbjct: 62  SEDLWMADNLPADAAVGVDPWCVSVDTAQKWIHTFSKKQQKLVQTSTNLVDEVWKNRPPP 121

Query: 152 EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV 211
           EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV
Sbjct: 122 EINPVIIHPLEYAGRSVEDKLKTLRAKLSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPV 181

Query: 212 VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS 271
           VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS
Sbjct: 182 VHAFAIVTLNSAFFYVEKRKVSDEVRLYMEKNGIEVRDYSAVITDVSSLASNQLNMSSSS 241

Query: 272 IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG 331
           IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG
Sbjct: 242 IDIAGSNGTKVESQSSGLIWADPAQCCYALYSKLNSDEVLLQQSPLALAKALKNSVELDG 301

Query: 332 LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE 391
           LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE
Sbjct: 302 LKNAHIRDGAAVVQYLVWLDKQLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLE 361

Query: 392 AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI 451
           AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI
Sbjct: 362 AFRASKKHFRGLSFPTISSVGPNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDI 421

Query: 452 TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTNGQQLDILARVPLWKDGLDYLHGT 511
           TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTN                       
Sbjct: 422 TRTVHFGLPSAHEKACYTAVLKGHITLGNARFPNGTN----------------------- 481

Query: 512 GHGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDGQQLDILARVPLWKDGLDYLHGTG 571
                                               GQQLDILARVPLWKDGLDYLHGTG
Sbjct: 482 ------------------------------------GQQLDILARVPLWKDGLDYLHGTG 541

Query: 572 HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT 631
           HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT
Sbjct: 542 HGIGSFLNVHEGPHLISFRPRARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADT 601

Query: 632 KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW 691
           KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW
Sbjct: 602 KFNFGDKGYLSFEHITWAPYQRKLINTSLLTSEELDWLNSYHSKCRDILAPYLDESEKAW 612

Query: 692 LNKATEPIPA 702
           LNKATEPIPA
Sbjct: 662 LNKATEPIPA 612

BLAST of CmaCh03G007720 vs. TAIR 10
Match: AT4G36760.1 (aminopeptidase P1 )

HSP 1 Score: 944.9 bits (2441), Expect = 3.7e-275
Identity = 454/701 (64.76%), Postives = 549/701 (78.32%), Query Frame = 0

Query: 1   MADTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQK 60
           M++ LS+LR LMASH+PPLDALVVPSEDYHQSEYVSARDKRREFVSGF+GSAGLALIT+K
Sbjct: 1   MSEILSSLRSLMASHSPPLDALVVPSEDYHQSEYVSARDKRREFVSGFSGSAGLALITKK 60

Query: 61  QALLWTDGRYFLQAIQQLSDQWKLMRIGEDPSEDLWMADNLPADAAVGVDPWCVSVDTAQ 120
           +A LWTDGRYFLQA+QQLSD+W LMR+GEDP  ++WM+DNLP +A +GVD WCVSVDTA 
Sbjct: 61  EARLWTDGRYFLQALQQLSDEWTLMRMGEDPLVEVWMSDNLPEEANIGVDSWCVSVDTAN 120

Query: 121 KWIHTFSKKQQKLVQTSTNLVDEVWKNRPPPEINPVIIHPLEYAGRSVEDKLKTLRAKLS 180
           +W  +F+KK QKL+ T+T+LVDEVWK+RPP E++PV++HPLE+AGRSV  K + LRAKL 
Sbjct: 121 RWGKSFAKKNQKLITTTTDLVDEVWKSRPPSEMSPVVVHPLEFAGRSVSHKFEDLRAKLK 180

Query: 181 REKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRLYM 240
           +E A GL++  LDEVAWLYNIRG+DV+YCPVVHAFAI+T +SAF YV+K+KVSDE   Y 
Sbjct: 181 QEGARGLVIAALDEVAWLYNIRGTDVAYCPVVHAFAILTTDSAFLYVDKKKVSDEANSYF 240

Query: 241 EKNGIEVRDYSAVITDVSSLASNQL--NMSSSSIDIAGSNGTKVESQSSGLIWADPAQCC 300
              G+EVR+Y+ VI+DV+ LAS++L  + +S ++    +   +++S     +W DPA CC
Sbjct: 241 NGLGVEVREYTDVISDVALLASDRLISSFASKTVQHEAAKDMEIDSDQPDRLWVDPASCC 300

Query: 301 YALYSKLNSDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDKQLQETY 360
           YALYSKL++++VLLQ SP++L+KALKN VEL+G+KNAH+RDGAAVVQYLVWLD Q+QE Y
Sbjct: 301 YALYSKLDAEKVLLQPSPISLSKALKNPVELEGIKNAHVRDGAAVVQYLVWLDNQMQELY 360

Query: 361 GASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVGPNAAII 420
           GASGYFLE +  +K KPS++ KLTEV+VSDKLE+ RASK+HFRGLSFPTISSVG NAA+I
Sbjct: 361 GASGYFLEAEASKK-KPSETSKLTEVTVSDKLESLRASKEHFRGLSFPTISSVGSNAAVI 420

Query: 421 HYGPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVLKGHITL 480
           HY P+ E CAE+DP+ +YL DSGAQYLDGTTDITRTVHFG PSAHEK CYTAV KGH+ L
Sbjct: 421 HYSPEPEACAEMDPDKIYLCDSGAQYLDGTTDITRTVHFGKPSAHEKECYTAVFKGHVAL 480

Query: 481 GNARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVP 540
           GNARFP GTN                                                  
Sbjct: 481 GNARFPKGTN-------------------------------------------------- 540

Query: 541 LQASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPRARNVPL 600
                    G  LDILAR PLWK GLDY HGTGHG+GS+L VHEGPH +SFRP ARNVPL
Sbjct: 541 ---------GYTLDILARAPLWKYGLDYRHGTGHGVGSYLCVHEGPHQVSFRPSARNVPL 600

Query: 601 QASMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQRKLINT 660
           QA+MTVTDEPGYYEDG FGIRLENVL+V +A+T+FNFGDKGYL FEHITWAPYQ KLI+ 
Sbjct: 601 QATMTVTDEPGYYEDGNFGIRLENVLVVNDAETEFNFGDKGYLQFEHITWAPYQVKLIDL 641

Query: 661 SLLTSEELDWLNSYHSKCRDILAPYLDESEKAWLNKATEPI 700
             LT EE+DWLN+YHSKC+DILAP+++++E  WL KATEP+
Sbjct: 661 DELTREEIDWLNTYHSKCKDILAPFMNQTEMEWLKKATEPV 641

BLAST of CmaCh03G007720 vs. TAIR 10
Match: AT3G05350.1 (Metallopeptidase M24 family protein )

HSP 1 Score: 513.1 bits (1320), Expect = 3.6e-145
Identity = 285/708 (40.25%), Postives = 409/708 (57.77%), Query Frame = 0

Query: 3   DTLSALRLLMASHNPPLDALVVPSEDYHQSEYVSARDKRREFVSGFTGSAGLALITQKQA 62
           + LS++R L +     +DA ++PS+D HQSE+++    RR ++SGFTGSAG A++T+ +A
Sbjct: 81  EKLSSIRRLFSEPGVGIDAYIIPSQDAHQSEFIAECYARRAYISGFTGSAGTAVVTKDKA 140

Query: 63  LLWTDGRYFLQAIQQLSDQWKLMRIGED--PSEDLWMADNLPADAAVGVDPWCVSVDTAQ 122
            LWTDGRYFLQA +QL+  W LMR G    P+   W+AD L     VG+DP+  S D A+
Sbjct: 141 ALWTDGRYFLQAEKQLNSSWILMRAGNPGVPTASEWIADVLAPGGRVGIDPFLFSADAAE 200

Query: 123 KWIHTFSKKQQKLVQT-STNLVDEVWK-NRPPPEINPVIIHPLEYAGRSVEDKLKTLRAK 182
           +     +KK  +LV   + NLVDE+WK +RP P    + IH L+YAG  V  KL +LR +
Sbjct: 201 ELKEVIAKKNHELVYLYNVNLVDEIWKDSRPKPPSRQIRIHDLKYAGLDVASKLLSLRNQ 260

Query: 183 LSREKAHGLIVVGLDEVAWLYNIRGSDVSYCPVVHAFAIVTLNSAFFYVEKRKVSDEVRL 242
           +       +++  LDE+AW+ N+RGSDV + PV++A+ IV ++ A  +V+  KV+ EV+ 
Sbjct: 261 IMDAGTSAIVISMLDEIAWVLNLRGSDVPHSPVMYAYLIVEVDQAQLFVDNSKVTVEVKD 320

Query: 243 YMEKNGIEVRDYSAVITDVSSLASN--QLNMSSSSIDIAGSNGTKVESQSSGLIWADPAQ 302
           +++  GIE+R Y +++  + SLA+   QL M  S++++A  +  K   +     +   A+
Sbjct: 321 HLKNAGIELRPYDSILQGIDSLAARGAQLLMDPSTLNVAIISTYKSACERYSRNFESEAK 380

Query: 303 CCYALYSKLN----SDEVLLQQSPLALAKALKNSVELDGLKNAHIRDGAAVVQYLVWLDK 362
                    +    +   +  QSP++ AKA+KN  EL G+KN+H+RD AA+  +  WL++
Sbjct: 381 VKTKFTDSSSGYTANPSGIYMQSPISWAKAIKNDAELKGMKNSHLRDAAALAHFWAWLEE 440

Query: 363 QLQETYGASGYFLEGDGVRKPKPSDSEKLTEVSVSDKLEAFRASKKHFRGLSFPTISSVG 422
           ++ +                     +  LTEV V+D+L  FR+ +  F   SF TIS  G
Sbjct: 441 EVHK---------------------NANLTEVDVADRLLEFRSMQDGFMDTSFDTISGSG 500

Query: 423 PNAAIIHYGPQAETCAELDPESMYLFDSGAQYLDGTTDITRTVHFGLPSAHEKACYTAVL 482
            N AIIHY P+ E+C+ +DP+ ++L DSGAQY+DGTTDITRTVHF  PSA EK C+T VL
Sbjct: 501 ANGAIIHYKPEPESCSRVDPQKLFLLDSGAQYVDGTTDITRTVHFSEPSAREKECFTRVL 560

Query: 483 KGHITLGNARFPNGTNGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRP 542
           +GHI L  A FP GT G  LD  AR  LWK GLDY HGTGHG+G+ LNVHEGP  ISFR 
Sbjct: 561 QGHIALDQAVFPEGTPGFVLDGFARSSLWKIGLDYRHGTGHGVGAALNVHEGPQSISFR- 620

Query: 543 RARNVPLQASMTVTDGQQLDILARVPLWKDGLDYLHGTGHGIGSFLNVHEGPHLISFRPR 602
                    +MT                                                
Sbjct: 621 -------YGNMT------------------------------------------------ 680

Query: 603 ARNVPLQASMTVTDEPGYYEDGAFGIRLENVLIVKNADTKFNFGDKGYLSFEHITWAPYQ 662
               PLQ  M V++EPGYYED AFGIR+EN+L V++A+T   FG   YL FE +T+ P Q
Sbjct: 681 ----PLQNGMIVSNEPGYYEDHAFGIRIENLLHVRDAETPNRFGGATYLGFEKLTFFPIQ 707

Query: 663 RKLINTSLLTSEELDWLNSYHSKCRDILAPYLDES-EKAWLNKATEPI 700
            K+++ SLL+  E+DWLNSYH++  + ++P L+ S  + WL   T P+
Sbjct: 741 TKMVDVSLLSDTEVDWLNSYHAEVWEKVSPLLEGSTTQQWLWNNTRPL 707