CmaCh03G006590 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G006590
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionaspartyl protease family protein 1-like
LocationCma_Chr03: 5540449 .. 5543471 (+)
RNA-Seq ExpressionCmaCh03G006590
SyntenyCmaCh03G006590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCACCCCGGAAAACTTTTGGATCCTCTAATCGGCGTCCATGGCGTCGACATTCACTTCCGCCGCCCAAATGCTACTGTTTCTTTCTATTTTCTTTCTCGCCGGCGAGGTGGTTTCGCTCAAGTTCAATATCCACCGTCGGTTTTCGGATTCGATTAAGGGGATTCTAAACTCTGATGGTTTACTGGAGAAACACACCCCTGGATATTACGCGGCTATGGTCCACCGCGATCGACTACTTCACGCCCGGCGATTGGCCACCTCTAAAAGTGAAACGCCGCTGACGTTCTCTTACGACAACCACACCTACCGATACGGCGGTTTGGGAGAGTATTGCACCTTACTTTCTCTGTTGATTTTCCGATTTACTTTGGCCGATTGCCTTGCTTTAATTTGTTTCCTGTTGGCTTTTCAGTTTCTACTACGCCAATATATCTGTGGGAACGCCGGAGTTAGGTTTCTTAGTGGCATTGGACACTGGAAGCGATTTGTCCTGGTTACCTTGTGAATGCAGCAAATGCCATACTTCCGTGGGCACTTCAGATGGGGAAGATGTATTTTCTTTCCGCCCTCTTCAATTTTCTCGGGAAACGAACAGTGCAAATTTTTGTAGTTGATGTTCTTATTTGTTTATGTTTCTTCAGATAACGTTGAATCATTACAGCCCAAATGCTTCAACAACGAGCAGAAGTGTAGCCTGCAGCAACTCTTTGTGCGAGCTTGCAGACCAATGCCCTTCAAACAAAAGTTCTTGTCCTTACAAGATTCATGATGCGTCTCCCAATACATCATCTTCTGGGTATTTGGTAGAAGATATCTTGCACTTGGCCACCAATGATTCACAATCCAAACCCGTTGATGCCAAGATTACTTTTGGGTGAGAACTGTTGAATCTCTCTGTCTGAATCAAATGCTTCTGAATTATGAACACATTTCGTTTAGTTCTTAATACATTTCTTTCTGTCAACATAAGAAACTAACCAAATACAAAATCTAGGGTAGTTTCATGATGTTTAGATGACTTGTTGTAAGTTGTTAAGAAGAATAGGTTCATACTGTTAGATGAAAGTCCCACATCGGTTAATTTAGGGAATGATCATGGGTTTATAATCCAGGAATACTATATTCATTGGTATGAGGCATTTTGAGGAAGTCCAAAGCAAAGCCACTTATGCTCAAGGTGGACAATATCATACCATTGTGGAGAGTTGTGTTCATCTAATGCATACATCTCCTACACATGTATTAATATTTGTTTGGTTCATCTTTCATTTTGATACTCGTGAATGTAGGGGAATTTTTTTTTCGAACTACGCAAATCTTTGTGTCTCTTTATTAAATTTTTGTTTGTGGGTGTGTGAGTACGATCGATCTTGCTTCGACTTTCGTTACAACAATGTCAACAATACTATCTTTCATAGAAACTATCTGCAATCTGGTGTGTTATTGCGTTGCTTTAAATGGGATTCTTGATGGCAGATGTGGTAAGGTCCAAACTGGTATATTTTCCAGCATCGCAATTAATGGGCTTCTTGGGCTTGGCATGGGGAAGATATCAGTTCCATCCCTCTTAGCAAGCCAAGATCTCACCCCAGATTCATTCTCCATGTGTTTTGGATATGATGGTTATGGGAACATCGATTTTGGAGACATAGGCCCATCAGACCAGAGAGAAACATCCCTCAATCCCCACTCGTAAGATCTTCTCACTTTGTAATCTCTGTCCTTCATTTTTCCTGGATAATAAGCTGTTTTTTCTAGCTGAATTCTGTTGTTTGGTGGCAGTTCATCCTACAATGTCACCATCATTCAGATAACTGTGGCAGGAAAACCCAACAATGTTGAATTTACTACAATTTTTGACTCTGGTTTCTCTTATACGTACCTCTCTGACCCAATTTACACTCTCGTTACTCAGAATGTAAGCTATTATCCTGGGAAGTGGCATATGAGTTCATGGAATCTCTGGTTTATATATAGAATTGTTGTGCAGATAGATGCAGCCATGGAATTAGACCGCGCTATATTTGATTCTGATTACCCATTTGAGTACTGCTACCAACTTTCGTAAGTAGATGATAATAATTTTTCGTCGTTTAACATTTGTATCATATTTGTGTACTGAGACCGTGTTATGGTGTATCAAGTTATTGTTCTTTCTATTTTCTTTGTATAAATATAGAATTTTTCCCTATCATTTAGAGGGTTGGTTTTAGCAAATATTCTTGAACAAGTTCTTCCGTTTCTGATTCCCATCCATTCCTATCCAGTCTAGGAACACACTTTCAACAACCGCATCTGAATTTTACAATGGATAGTGGCTATATTTTTGGCGTTGTTTCTATGTTTGTTGCCATTCCTACCGATGTGAGTTATCTGAACAGTTCAAGTCTTTTGCTTTTTAAGCTTTCTTTCAGTAATCCTTTGGGTTTTTCTTGATCTGGGTGTTCGCGTGGGCTTCAGGACAAGGGTGGACATGCCATTTGTCTTACCATTGCCAAGAGCACTGATATTAATCTCTTCGGACGTGAGTGCCCATCTCTGCTATTTGTTGATGTTGGAAGAAAAAGATTATGAACTTAAAAGCTTCTCCCTTTCTGGCAGTGAATTTCATGGCTGGTCATCGTATCGTCTTCAATCGTGAAAAGATGGTTCTGGGCTGGAATGAAGTGAACTGTTCAGTATTTCTTCACCTCAAAACCTCTCTTCGATTTTCTGGCTTTGTTCATTGTCGGTGATCGCTTGTTTTTTTTTTAAATTTTTAAATCCAGGTTTCGACAATGGCGCCGACAACTCCCCTCCGTCCAACTCTCCGCCGGCCGACGCACCATCACCGTCCGGCCACTCTCCTCCGGCACCTTCTACCACTAGAACAAGCAACAGCACGCAGTTATCGCCGGGAATTGGGACGGGTGATGCGACGCAGTTAAACCCGCTTGCCTGTCTCTCTGTTGTCCTTCTTGCTATTTTGTCTGTTGTTTGACTTTCATTATTTATTTCGTATATTTTATTTTAAAAATAAA

mRNA sequence

CTTCACCCCGGAAAACTTTTGGATCCTCTAATCGGCGTCCATGGCGTCGACATTCACTTCCGCCGCCCAAATGCTACTGTTTCTTTCTATTTTCTTTCTCGCCGGCGAGGTGGTTTCGCTCAAGTTCAATATCCACCGTCGGTTTTCGGATTCGATTAAGGGGATTCTAAACTCTGATGGTTTACTGGAGAAACACACCCCTGGATATTACGCGGCTATGGTCCACCGCGATCGACTACTTCACGCCCGGCGATTGGCCACCTCTAAAAGTGAAACGCCGCTGACGTTCTCTTACGACAACCACACCTACCGATACGGCGGTTTGGGAGATTTCTACTACGCCAATATATCTGTGGGAACGCCGGAGTTAGGTTTCTTAGTGGCATTGGACACTGGAAGCGATTTGTCCTGGTTACCTTGTGAATGCAGCAAATGCCATACTTCCGTGGGCACTTCAGATGGGGAAGATATAACGTTGAATCATTACAGCCCAAATGCTTCAACAACGAGCAGAAGTGTAGCCTGCAGCAACTCTTTGTGCGAGCTTGCAGACCAATGCCCTTCAAACAAAAGTTCTTGTCCTTACAAGATTCATGATGCGTCTCCCAATACATCATCTTCTGGGTATTTGGTAGAAGATATCTTGCACTTGGCCACCAATGATTCACAATCCAAACCCGTTGATGCCAAGATTACTTTTGGATGTGGTAAGGTCCAAACTGGTATATTTTCCAGCATCGCAATTAATGGGCTTCTTGGGCTTGGCATGGGGAAGATATCAGTTCCATCCCTCTTAGCAAGCCAAGATCTCACCCCAGATTCATTCTCCATGTGTTTTGGATATGATGGTTATGGGAACATCGATTTTGGAGACATAGGCCCATCAGACCAGAGAGAAACATCCCTCAATCCCCACTCTTCATCCTACAATGTCACCATCATTCAGATAACTGTGGCAGGAAAACCCAACAATGTTGAATTTACTACAATTTTTGACTCTGGTTTCTCTTATACGTACCTCTCTGACCCAATTTACACTCTCGTTACTCAGAATATAGATGCAGCCATGGAATTAGACCGCGCTATATTTGATTCTGATTACCCATTTGAGTACTGCTACCAACTTTCTCTAGGAACACACTTTCAACAACCGCATCTGAATTTTACAATGGATAGTGGCTATATTTTTGGCGTTGTTTCTATGTTTGTTGCCATTCCTACCGATGACAAGGGTGGACATGCCATTTGTCTTACCATTGCCAAGAGCACTGATATTAATCTCTTCGGACTGAATTTCATGGCTGGTCATCGTATCGTCTTCAATCGTGAAAAGATGGTTCTGGGCTGGAATGAAGTGAACTGTTTCGACAATGGCGCCGACAACTCCCCTCCGTCCAACTCTCCGCCGGCCGACGCACCATCACCGTCCGGCCACTCTCCTCCGGCACCTTCTACCACTAGAACAAGCAACAGCACGCAGTTATCGCCGGGAATTGGGACGGGTGATGCGACGCAGTTAAACCCGCTTGCCTGTCTCTCTGTTGTCCTTCTTGCTATTTTGTCTGTTGTTTGACTTTCATTATTTATTTCGTATATTTTATTTTAAAAATAAA

Coding sequence (CDS)

ATGGCGTCGACATTCACTTCCGCCGCCCAAATGCTACTGTTTCTTTCTATTTTCTTTCTCGCCGGCGAGGTGGTTTCGCTCAAGTTCAATATCCACCGTCGGTTTTCGGATTCGATTAAGGGGATTCTAAACTCTGATGGTTTACTGGAGAAACACACCCCTGGATATTACGCGGCTATGGTCCACCGCGATCGACTACTTCACGCCCGGCGATTGGCCACCTCTAAAAGTGAAACGCCGCTGACGTTCTCTTACGACAACCACACCTACCGATACGGCGGTTTGGGAGATTTCTACTACGCCAATATATCTGTGGGAACGCCGGAGTTAGGTTTCTTAGTGGCATTGGACACTGGAAGCGATTTGTCCTGGTTACCTTGTGAATGCAGCAAATGCCATACTTCCGTGGGCACTTCAGATGGGGAAGATATAACGTTGAATCATTACAGCCCAAATGCTTCAACAACGAGCAGAAGTGTAGCCTGCAGCAACTCTTTGTGCGAGCTTGCAGACCAATGCCCTTCAAACAAAAGTTCTTGTCCTTACAAGATTCATGATGCGTCTCCCAATACATCATCTTCTGGGTATTTGGTAGAAGATATCTTGCACTTGGCCACCAATGATTCACAATCCAAACCCGTTGATGCCAAGATTACTTTTGGATGTGGTAAGGTCCAAACTGGTATATTTTCCAGCATCGCAATTAATGGGCTTCTTGGGCTTGGCATGGGGAAGATATCAGTTCCATCCCTCTTAGCAAGCCAAGATCTCACCCCAGATTCATTCTCCATGTGTTTTGGATATGATGGTTATGGGAACATCGATTTTGGAGACATAGGCCCATCAGACCAGAGAGAAACATCCCTCAATCCCCACTCTTCATCCTACAATGTCACCATCATTCAGATAACTGTGGCAGGAAAACCCAACAATGTTGAATTTACTACAATTTTTGACTCTGGTTTCTCTTATACGTACCTCTCTGACCCAATTTACACTCTCGTTACTCAGAATATAGATGCAGCCATGGAATTAGACCGCGCTATATTTGATTCTGATTACCCATTTGAGTACTGCTACCAACTTTCTCTAGGAACACACTTTCAACAACCGCATCTGAATTTTACAATGGATAGTGGCTATATTTTTGGCGTTGTTTCTATGTTTGTTGCCATTCCTACCGATGACAAGGGTGGACATGCCATTTGTCTTACCATTGCCAAGAGCACTGATATTAATCTCTTCGGACTGAATTTCATGGCTGGTCATCGTATCGTCTTCAATCGTGAAAAGATGGTTCTGGGCTGGAATGAAGTGAACTGTTTCGACAATGGCGCCGACAACTCCCCTCCGTCCAACTCTCCGCCGGCCGACGCACCATCACCGTCCGGCCACTCTCCTCCGGCACCTTCTACCACTAGAACAAGCAACAGCACGCAGTTATCGCCGGGAATTGGGACGGGTGATGCGACGCAGTTAAACCCGCTTGCCTGTCTCTCTGTTGTCCTTCTTGCTATTTTGTCTGTTGTTTGA

Protein sequence

MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLGLGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQLSPGIGTGDATQLNPLACLSVVLLAILSVV
Homology
BLAST of CmaCh03G006590 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.6e-110
Identity = 220/469 (46.91%), Postives = 296/469 (63.11%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDNH 88
           F  H RFSD + G+L  DGL  + +  YY  M HRDRL+  RRLA ++ ++ +TFS  N 
Sbjct: 35  FEFHHRFSDQVVGVLPGDGLPNRDSSKYYRVMAHRDRLIRGRRLA-NEDQSLVTFSDGNE 94

Query: 89  TYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCHTSVGTSDGEDITLNH 148
           T R   LG  +YAN++VGTP   F+VALDTGSDL WLPC+C+ C   +    G  + LN 
Sbjct: 95  TVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLFWLPCDCTNCVRELKAPGGSSLDLNI 154

Query: 149 YSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATND 208
           YSPNAS+TS  V C+++LC   D+C S +S CPY+I   S  TSS+G LVED+LHL +ND
Sbjct: 155 YSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSND 214

Query: 209 SQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQDLTPDSFSMCFG 268
             SK + A++TFGCG+VQTG+F    A NGL GLG+  ISVPS+LA + +  +SFSMCFG
Sbjct: 215 KSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFG 274

Query: 269 YDGYGNIDFGDIGPSDQRETSLN---PHSSSYNVTIIQITVAGKPNNVEFTTIFDSGFSY 328
            DG G I FGD G  DQRET LN   PH  +YN+T+ +I+V G   ++EF  +FDSG S+
Sbjct: 275 NDGAGRISFGDKGSVDQRETPLNIRQPH-PTYNITVTKISVGGNTGDLEFDAVFDSGTSF 334

Query: 329 TYLSDPIYTLVTQNIDA-AMELDRAIFDSDYPFEYCYQLSLG-THFQQPHLNFTMDSGYI 388
           TYL+D  YTL++++ ++ A++      DS+ PFEYCY LS     FQ P +N TM  G  
Sbjct: 335 TYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVNLTMKGGSS 394

Query: 389 FGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCF 448
           + V    V IP  D      CL I K  DI++ G NFM G+R+VF+REK++LGW E +C+
Sbjct: 395 YPVYHPLVVIPMKDT--DVYCLAIMKIEDISIIGQNFMTGYRVVFDREKLILGWKESDCY 454

Query: 449 DN-------GADNSPPSNSPPADAPSPSGHSPPA--PSTTRTSNSTQLS 483
                     ++ S  S  PPA +  P   + P+  P+T+ TS +  LS
Sbjct: 455 TGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTSTTSAAYSLS 499

BLAST of CmaCh03G006590 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.5e-62
Identity = 185/514 (35.99%), Postives = 263/514 (51.17%), Query Frame = 0

Query: 7   SAAQMLLFLSIFFLAGEVVSLKFN--IHRRFSD----SIKGILNSDGLLEKHTPGYYAAM 66
           S +  LLF  +F    E ++  F+  +  RFSD    SIK   +SD L  K +  YY  +
Sbjct: 3   SRSAFLLFCVLFLATEETLASLFSSRLIHRFSDEGRASIKTPSSSDSLPNKQSLEYYRLL 62

Query: 67  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGG-LGDFYYANISVGTPELGFLVALDTG 126
              D     +R+        L  S  + T   G   G  +Y  I +GTP + FLVALDTG
Sbjct: 63  AESD--FRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTG 122

Query: 127 SDLSWLPCECSKC-------HTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQ 186
           S+L W+PC C +C       ++S+ T D     LN Y+P++S+TS+   CS+ LC+ A  
Sbjct: 123 SNLLWIPCNCVQCAPLTSTYYSSLATKD-----LNEYNPSSSSTSKVFLCSHKLCDSASD 182

Query: 187 CPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQ-----SKPVDAKITFGCGKVQT 246
           C S K  CPY ++  S NTSSSG LVEDILHL  N +      S  V A++  GCGK Q+
Sbjct: 183 CESPKEQCPYTVNYLSGNTSSSGLLVEDILHLTYNTNNRLMNGSSSVKARVVIGCGKKQS 242

Query: 247 GIF-SSIAINGLLGLGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRE 306
           G +   +A +GL+GLG  +ISVPS L+   L  +SFS+CF  +  G I FGD+GPS Q+ 
Sbjct: 243 GDYLDGVAPDGLMGLGPAEISVPSFLSKAGLMRNSFSLCFDEEDSGRIYFGDMGPSIQQS 302

Query: 307 TS-LNPHSSSYNVTIIQITVAGKPNN----VEFTTIFDSGFSYTYLSDPIYTLVTQNIDA 366
           T  L   ++ Y+  I+ +      N+      FTT  DSG S+TYL + IY  V   ID 
Sbjct: 303 TPFLQLDNNKYSGYIVGVEACCIGNSCLKQTSFTTFIDSGQSFTYLPEEIYRKVALEIDR 362

Query: 367 AMELDRAIFDSDYPFEYCYQLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHA 426
            +      F+    +EYCY+ S         L F+ ++ +   V+   + +    +G   
Sbjct: 363 HINATSKNFEG-VSWEYCYESSAEPKVPAIKLKFSHNNTF---VIHKPLFVFQQSQGLVQ 422

Query: 427 ICLTIAKS--TDINLFGLNFMAGHRIVFNREKMVLGWNEVNCFDNGAD---NSPPSNSPP 480
            CL I+ S    I   G N+M G+R+VF+RE M LGW+   C ++  +    SP S S P
Sbjct: 423 FCLPISPSGQEGIGSIGQNYMRGYRMVFDRENMKLGWSPSKCQEDKIEPPQASPGSTSSP 482

BLAST of CmaCh03G006590 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 4.1e-26
Identity = 118/468 (25.21%), Postives = 194/468 (41.45%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVS--LKFNIHRRFSDSIKGILNSDGLLEKHTPGYYA 60
           M +T   +    +   +F L  +VVS    FN+  +F+   K +      L+ H      
Sbjct: 1   MVTTMDPSRISRIVAVVFVLVIQVVSGNFVFNVTHKFAGKEKQLSE----LKSH------ 60

Query: 61  AMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDT 120
                D   HAR LA       L    D+     G     Y+  I +G+P   + V +DT
Sbjct: 61  -----DSFRHARMLA----NIDLPLGGDSRADSIG----LYFTKIKLGSPPKEYYVQVDT 120

Query: 121 GSDLSWLPC-ECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPS-- 180
           GSD+ W+ C  C KC   V T  G  I L+ Y    S+TS++V C +  C    Q  +  
Sbjct: 121 GSDILWVNCAPCPKC--PVKTDLG--IPLSLYDSKTSSTSKNVGCEDDFCSFIMQSETCG 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDI-LHLATNDSQSKPVDAKITFGCGKVQTGIF--SS 240
            K  C Y +     +TS   ++ ++I L   T + ++ P+  ++ FGCGK Q+G    + 
Sbjct: 181 AKKPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQTD 240

Query: 241 IAINGLLGLGMGKISVPSLLASQDLTPDSFSMCF-GYDGYGNIDFGDIGPSDQRETSLNP 300
            A++G++G G    S+ S LA+   T   FS C    +G G    G++     + T + P
Sbjct: 241 SAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGGIFAVGEVESPVVKTTPIVP 300

Query: 301 HSSSYNVTIIQITVAGKP---------NNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAA 360
           +   YNV +  + V G P          N +  TI DSG +  YL   +Y  + + I A 
Sbjct: 301 NQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKITAK 360

Query: 361 MELDRAIFDSDYPFEYCYQLSLGTHFQQPHLNFTMDSG---------YIFGVVSMFVAIP 420
            ++   +    +    C+  +  T    P +N   +           Y+F +        
Sbjct: 361 QQVKLHMVQETFA---CFSFTSNTDKAFPVVNLHFEDSLKLSVYPHDYLFSLREDMYCFG 420

Query: 421 TDDKGGHAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNC 442
               G     +T     D+ L G   ++   +V++ E  V+GW + NC
Sbjct: 421 WQSGG-----MTTQDGADVILLGDLVLSNKLVVYDLENEVIGWADHNC 433

BLAST of CmaCh03G006590 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 1.6e-25
Identity = 96/361 (26.59%), Postives = 162/361 (44.88%), Query Frame = 0

Query: 99  YYANISVGTPELGFLVALDTGSDLSWLPCE-CSKCHTSVGTSDGEDITLNHYSPNASTTS 158
           Y+  I +G+P   + V +DTGSD+ W+ C+ C KC T        +  L+ +  NAS+TS
Sbjct: 74  YFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNL----NFRLSLFDMNASSTS 133

Query: 159 RSVACSNSLCELADQCPSNKSS--CPYKIHDASPNTSSSGYLVEDILHL--ATNDSQSKP 218
           + V C +  C    Q  S + +  C Y I  A  +T S G  + D+L L   T D ++ P
Sbjct: 134 KKVGCDDDFCSFISQSDSCQPALGCSYHIVYADEST-SDGKFIRDMLTLEQVTGDLKTGP 193

Query: 219 VDAKITFGCGKVQTGIFSS--IAINGLLGLGMGKISVPSLLASQDLTPDSFSMCF-GYDG 278
           +  ++ FGCG  Q+G   +   A++G++G G    SV S LA+       FS C     G
Sbjct: 194 LGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDNVKG 253

Query: 279 YGNIDFGDIGPSDQRETSLNPHSSSYNVTIIQITVAGK----PNNV--EFTTIFDSGFSY 338
            G    G +     + T + P+   YNV ++ + V G     P ++     TI DSG + 
Sbjct: 254 GGIFAVGVVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDLPRSIVRNGGTIVDSGTTL 313

Query: 339 TYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQLSLGTHFQQPHLNFTMDSGYIFG 398
            Y    +Y  + + I A   +   I +  +    C+  S       P ++F  +      
Sbjct: 314 AYFPKVLYDSLIETILARQPVKLHIVEETF---QCFSFSTNVDEAFPPVSFEFEDSVKLT 373

Query: 399 VVSMFVAIPTDDK----GGHAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVN 442
           V         +++    G  A  LT  + +++ L G   ++   +V++ +  V+GW + N
Sbjct: 374 VYPHDYLFTLEEELYCFGWQAGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHN 426

BLAST of CmaCh03G006590 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 6.7e-21
Identity = 103/381 (27.03%), Postives = 168/381 (44.09%), Query Frame = 0

Query: 99  YYANISVGTPELG--FLVALDTGSDLSWLPCE--CSKCHTSVGTSDGEDITLNHYSPNAS 158
           YY  I VG PE G  + + +DTGS+L+W+ C+  C+ C      + G +        N  
Sbjct: 203 YYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCTSC------AKGANQLYKPRKDNLV 262

Query: 159 TTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPV 218
            +S +        +L + C  N   C Y+I  A  ++ S G L +D  HL  ++      
Sbjct: 263 RSSEAFCVEVQRNQLTEHC-ENCHQCDYEIEYAD-HSYSMGVLTKDKFHLKLHNGSL--A 322

Query: 219 DAKITFGCGKVQTGIF--SSIAINGLLGLGMGKISVPSLLASQDLTPDSFSMCFGYD--G 278
           ++ I FGCG  Q G+   + +  +G+LGL   KIS+PS LAS+ +  +    C   D  G
Sbjct: 323 ESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGHCLASDLNG 382

Query: 279 YGNIDFG-DIGPSDQRETSLNPHSSSYNVTIIQIT----------VAGKPNNVEFTTIFD 338
            G I  G D+ PS         H S  +   +Q+T          + G+   V    +FD
Sbjct: 383 EGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGRVG-KVLFD 442

Query: 339 SGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQLSLGTHFQQPHLN----- 398
           +G SYTY  +  Y+ +  ++     L+    DSD     C++    T+F    L+     
Sbjct: 443 TGSSYTYFPNQAYSQLVTSLQEVSGLELTRDDSDETLPICWRAK--TNFPFSSLSDVKKF 502

Query: 399 ---FTMDSGYIFGVVSMFVAIPTDD------KGGHAICLTIAKSTDIN-----LFGLNFM 442
               T+  G  + ++S  + I  +D      KG   +CL I   + ++     + G   M
Sbjct: 503 FRPITLQIGSKWLIISRKLLIQPEDYLIISNKGN--VCLGILDGSSVHDGSTIILGDISM 562

BLAST of CmaCh03G006590 vs. ExPASy TrEMBL
Match: A0A6J1IJH6 (aspartyl protease family protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478000 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.6e-294
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
           QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM
Sbjct: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480

Query: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           LSPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmaCh03G006590 vs. ExPASy TrEMBL
Match: A0A6J1GFR6 (aspartyl protease family protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453524 PE=3 SV=1)

HSP 1 Score: 989.6 bits (2557), Expect = 5.0e-285
Identity = 496/510 (97.25%), Postives = 502/510 (98.43%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYT VTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
           QL L T FQQPHLNFTMDSGYIFGV+SMFVAIPTDDKGG+AICLTIAKSTDINLFGLNFM
Sbjct: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSG SPPAPST +TSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480

Query: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmaCh03G006590 vs. ExPASy TrEMBL
Match: A0A6J1BTF7 (aspartyl protease family protein 1-like OS=Momordica charantia OX=3673 GN=LOC111005407 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 1.3e-179
Identity = 345/547 (63.07%), Postives = 400/547 (73.13%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEV-----VSLKFNIHRRFSDSIKGILNSDGLLEKHTPG 60
           MA TF+S AQMLLFLS+F LAGE+      S KF+IH RFSDSIKGIL+S+GL EK +PG
Sbjct: 1   MAWTFSSGAQMLLFLSVFLLAGELRSGEAGSFKFSIHHRFSDSIKGILDSEGLPEKQSPG 60

Query: 61  YYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVA 120
           YYA MVHRDRL+H RRLAT+  + PLTF Y N T+  G LG  YYANISVGTPEL FLVA
Sbjct: 61  YYATMVHRDRLVHGRRLATTNGDPPLTFVYGNDTFLIGNLGFLYYANISVGTPELSFLVA 120

Query: 121 LDTGSDLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPS 180
           LDTGSDL WLPCECS C T + T++G    LNHYSP  STTS SV CSNSLCELA+QC S
Sbjct: 121 LDTGSDLFWLPCECSSCLTYLNTTNGGKFGLNHYSPKDSTTSTSVPCSNSLCELANQCSS 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFS-SIA 240
             S+CPY+I+  S NTSSSGYLV+D+LHLAT+D Q KPVDAKITFGCGK+QTGIF+ S A
Sbjct: 181 TTSTCPYEINYLSANTSSSGYLVQDVLHLATDDKQLKPVDAKITFGCGKIQTGIFADSAA 240

Query: 241 INGLLGLGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNP--H 300
            NGL+GLGM KISVPS LASQ LT DSFSMCFGYDG G IDFGDIG S QRET  N   +
Sbjct: 241 PNGLIGLGMEKISVPSFLASQGLTSDSFSMCFGYDGQGRIDFGDIGTSGQRETPFNTMVN 300

Query: 301 SSSYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDS 360
             SYNVT  QI V GK NN++F+ IFDSG S++Y++DP+Y+L+ + +DA M+L+R  FD 
Sbjct: 301 FPSYNVTFTQIIVGGKSNNLQFSAIFDSGTSFSYITDPVYSLIAEQMDAGMKLERVKFDP 360

Query: 361 DYPFEYCYQLSLGTH-FQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTD 420
           D+PFEYCYQL    + F  P LNFTM  G  +G +  FV +PTD+ GG A CL I KSTD
Sbjct: 361 DFPFEYCYQLPPNANSFSHPRLNFTMKGGDNYGTLDPFVVVPTDNSGGLAACLGIVKSTD 420

Query: 421 -INLFGLNFMAGHRIVFNREKMVLGWNEVNCFDNG----------ADNSPPSNSPPADA- 480
            I+L G NFM G+RI+FNREKMVLGW E +C+DNG          ADNSPPS+SPP D+ 
Sbjct: 421 PIDLIGQNFMTGYRIIFNREKMVLGWTESDCYDNGAATPSDNSPPADNSPPSDSPPTDSS 480

Query: 481 -------PS---------PSGHSPPAPSTTRTSNSTQLSPGIGTGDATQLNPLACLSVVL 511
                  PS         PSG SPPAPST   SN TQ  P IG GDAT+LNPL C+ V +
Sbjct: 481 PPTDSSPPSDSPQSGDSPPSGDSPPAPSTAGGSNGTQPLPRIGAGDATRLNPLGCVFVAI 540

BLAST of CmaCh03G006590 vs. ExPASy TrEMBL
Match: A0A0A0KGS0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507240 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 1.1e-178
Identity = 332/516 (64.34%), Postives = 387/516 (75.00%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLA-----GEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPG 60
           MA TF+S  QMLL LS+FFLA     G   S KF IH RFSDSIK I  S+GL EKHTPG
Sbjct: 1   MAWTFSSGDQMLLVLSVFFLAGGLRSGHAASFKFTIHHRFSDSIKEIFGSEGLPEKHTPG 60

Query: 61  YYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVA 120
           YYAAMVHRDRLLH R LAT+  +TPL FSY N TY   GLG+ YYAN+S+GTP L FLVA
Sbjct: 61  YYAAMVHRDRLLHGRNLATTNGDTPLMFSYGNETYELSGLGNLYYANVSIGTPGLYFLVA 120

Query: 121 LDTGSDLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPS 180
           LDTGSDL WLPCEC+KC T +   D     LNHYS NAS+TS  V CS+SLCELA+QC S
Sbjct: 121 LDTGSDLFWLPCECTKCPTYLTKRDNGKFWLNHYSSNASSTSIRVPCSSSLCELANQCSS 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSI-A 240
           NKSSCPY+ H  S N+SS+GYLV+DILH+AT+DSQ KPVD K+T GCGKVQTG FS++ A
Sbjct: 181 NKSSCPYQTHYLSENSSSAGYLVQDILHMATDDSQLKPVDVKVTLGCGKVQTGKFSNVTA 240

Query: 241 INGLLGLGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSS 300
            NGL+GLGMGK+SVPS LASQ LT DSFSMCFGY GYG IDFGDIGP  QRET  NP S 
Sbjct: 241 PNGLIGLGMGKVSVPSFLASQGLTTDSFSMCFGYYGYGRIDFGDIGPVGQRETPFNPASL 300

Query: 301 SYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDY 360
           SYNVTI+QI V  +P NV  T I DSG S+TYL+DP Y+++T+N+DAAMEL+R   DSD+
Sbjct: 301 SYNVTILQIIVTNRPTNVHLTAIIDSGASFTYLTDPFYSIITENMDAAMELERIKSDSDF 360

Query: 361 PFEYCYQLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINL 420
           PFEYCY+LSL T FQQP+LNFTM+ G  F V++ +V++ TDD  G A+CL I KSTDIN+
Sbjct: 361 PFEYCYRLSLATIFQQPNLNFTMEGGRKFDVITSYVSVDTDD--GPALCLAIVKSTDINV 420

Query: 421 FGLNFMAGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTR 480
            G NF  G+R+VFNREKM LGW EV+C      +S  +N+   D+P PSG S P  ST R
Sbjct: 421 IGHNFFGGYRVVFNREKMTLGWKEVDC------DSYDANTSSDDSPPPSGDSSPTTSTPR 480

Query: 481 TSNSTQLSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SNSTQ SP IG GDA  LNP+  L VV+L IL V+
Sbjct: 481 KSNSTQPSPEIGAGDAMLLNPIVSLCVVILVILYVI 508

BLAST of CmaCh03G006590 vs. ExPASy TrEMBL
Match: A0A1S3B2V8 (aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485160 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 1.0e-176
Identity = 330/517 (63.83%), Postives = 388/517 (75.05%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLA-----GEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPG 60
           MA TF+S  QMLL LS+FFLA     G   S KF IH RFSDSIK I  S+GL EKHTPG
Sbjct: 1   MAWTFSSGDQMLLVLSVFFLAGGLRSGHAASFKFTIHHRFSDSIKEIFGSEGLAEKHTPG 60

Query: 61  YYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVA 120
           YYAAMVHRDRLLH R LA +  +TPL FSY N TYR  GLG+ YYAN+S+GTP L FLVA
Sbjct: 61  YYAAMVHRDRLLHGRNLANTNGDTPLMFSYGNETYRISGLGNLYYANVSIGTPGLYFLVA 120

Query: 121 LDTGSDLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPS 180
           LDTGS+L WLPCEC+KC T + T D E   LNHYS NAS+TS  V CS+SLCE A+QC S
Sbjct: 121 LDTGSNLFWLPCECTKCRTYLTTRDNEKFWLNHYSSNASSTSIRVPCSSSLCEHANQCSS 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSI-A 240
           N+SSCPYK H  S N+SS+GYLV+DILH+AT+DSQ KPVDAK+T GCGKVQTG F++  A
Sbjct: 181 NRSSCPYKTHYPSQNSSSAGYLVQDILHMATDDSQLKPVDAKVTLGCGKVQTGKFANFTA 240

Query: 241 INGLLGLGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSS 300
            NGL+GLGMGK+SVPS LASQ LT DSFSMCFGY G G IDFGDIG   QRET LNP S 
Sbjct: 241 ANGLIGLGMGKLSVPSFLASQGLTADSFSMCFGYYGNGRIDFGDIGSVGQRETPLNPTSL 300

Query: 301 SYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDY 360
           SYNVTI+QI V  +P NV FT I D+G S+TYL+DP Y++++  +D A+EL+R   DSD+
Sbjct: 301 SYNVTILQIIVTNRPTNVHFTAIIDTGSSFTYLTDPFYSIISDKMDEAIELERIASDSDF 360

Query: 361 PFEYCYQLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINL 420
           PFEYCYQLS+GT F QP +NFTM+ G  F V+S +V I TDD  G A+CL I KSTDIN+
Sbjct: 361 PFEYCYQLSMGTIFLQPTMNFTMEGGRKFDVISSYVMINTDD--GIALCLAIVKSTDINV 420

Query: 421 FGLNFMAGHRIVFNREKMVLGWNE-VNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTT 480
            G N ++G+R+VFNREKM LGW E V+C + GAD      +P  ++P P G SPP  S+ 
Sbjct: 421 IGSNLLSGYRVVFNREKMTLGWKEVVDCDNYGAD------TPSGESPPPFGGSPPPTSSP 480

Query: 481 RTSNSTQLSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           R SNSTQ SP IGTGDA +LNP+  L VV+L ILSVV
Sbjct: 481 RKSNSTQPSPEIGTGDAMRLNPIVSLCVVILVILSVV 509

BLAST of CmaCh03G006590 vs. NCBI nr
Match: XP_022977812.1 (aspartyl protease family protein 1-like [Cucurbita maxima])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.6e-293
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
           QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM
Sbjct: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480

Query: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           LSPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmaCh03G006590 vs. NCBI nr
Match: XP_022950419.1 (aspartyl protease family protein 1-like [Cucurbita moschata])

HSP 1 Score: 989.6 bits (2557), Expect = 1.0e-284
Identity = 496/510 (97.25%), Postives = 502/510 (98.43%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYT VTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
           QL L T FQQPHLNFTMDSGYIFGV+SMFVAIPTDDKGG+AICLTIAKSTDINLFGLNFM
Sbjct: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSG SPPAPST +TSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480

Query: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmaCh03G006590 vs. NCBI nr
Match: XP_023521822.1 (aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 3.5e-277
Identity = 482/510 (94.51%), Postives = 493/510 (96.67%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLH RRL TSKSETPLTFSYDNHTY YG LGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHGRRLVTSKSETPLTFSYDNHTYHYGSLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DL WLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLFWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLAT+DSQSKPVDAKITFGCGKVQTGIFS++A NGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATDDSQSKPVDAKITFGCGKVQTGIFSNVAANGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSL P+SSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLKPNSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKP+NVEFT IFDSG SYTYL+DPIYTLVTQ IDAAMELDR IFDSDYPFEYCY
Sbjct: 301 IQITVAGKPSNVEFTAIFDSGSSYTYLADPIYTLVTQKIDAAMELDRFIFDSDYPFEYCY 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
           QL LGT FQQPHLNFTMDSGYIFGV+SMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM
Sbjct: 361 QLPLGTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSG SPPAPSTTRTSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTTRTSNSTQ 480

Query: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SPGIGTGDATQLNPLACLSVVLLAIL +V
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILFIV 510

BLAST of CmaCh03G006590 vs. NCBI nr
Match: XP_023544616.1 (aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.7 bits (2342), Expect = 8.7e-260
Identity = 462/510 (90.59%), Postives = 477/510 (93.53%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLH RRL TSKSETPLTFSYDNHTY YG LGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHGRRLVTSKSETPLTFSYDNHTYHYGSLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DL WLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLFWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLAT+DSQSKPVDAKITFGCGKVQTGIFS++A NGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATDDSQSKPVDAKITFGCGKVQTGIFSNVAANGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSL P+SSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLKPNSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKP+NVEFT IFDSG SYTYL+DPIYTLVTQ +   +    +  DSD    + +
Sbjct: 301 IQITVAGKPSNVEFTAIFDSGSSYTYLADPIYTLVTQKVSYYLGNKHSRQDSD---SHLF 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
             SLGT FQQPHLNFTMDSGYIFGV+SMFVAIPTD  GGHAICLTIAKSTDINLFGLNFM
Sbjct: 361 LSSLGTLFQQPHLNFTMDSGYIFGVISMFVAIPTD--GGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSG SPPAPSTTRTSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTTRTSNSTQ 480

Query: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SPGIGTGDATQLNPLACLSVVLLAIL VV
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILFVV 505

BLAST of CmaCh03G006590 vs. NCBI nr
Match: KAG6603890.1 (Peroxidase 64, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 886.7 bits (2290), Expect = 9.4e-254
Identity = 450/487 (92.40%), Postives = 455/487 (93.43%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYT VTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420
           QL LGT FQQPHLNFTMDSGYIFGV+SMFVAIPTDDKGG+AICLTIAKSTDINLFG    
Sbjct: 361 QLPLGTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGR--- 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480
                                FDNGADNSPPSNSPPADAPSPSG SPPAPST RTSNSTQ
Sbjct: 421 ---------------------FDNGADNSPPSNSPPADAPSPSGDSPPAPSTPRTSNSTQ 463

Query: 481 LSPGIGT 488
            SPGIGT
Sbjct: 481 SSPGIGT 463

BLAST of CmaCh03G006590 vs. TAIR 10
Match: AT2G17760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 220/469 (46.91%), Postives = 296/469 (63.11%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDNH 88
           F  H RFSD + G+L  DGL  + +  YY  M HRDRL+  RRLA ++ ++ +TFS  N 
Sbjct: 35  FEFHHRFSDQVVGVLPGDGLPNRDSSKYYRVMAHRDRLIRGRRLA-NEDQSLVTFSDGNE 94

Query: 89  TYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCHTSVGTSDGEDITLNH 148
           T R   LG  +YAN++VGTP   F+VALDTGSDL WLPC+C+ C   +    G  + LN 
Sbjct: 95  TVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLFWLPCDCTNCVRELKAPGGSSLDLNI 154

Query: 149 YSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATND 208
           YSPNAS+TS  V C+++LC   D+C S +S CPY+I   S  TSS+G LVED+LHL +ND
Sbjct: 155 YSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSND 214

Query: 209 SQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQDLTPDSFSMCFG 268
             SK + A++TFGCG+VQTG+F    A NGL GLG+  ISVPS+LA + +  +SFSMCFG
Sbjct: 215 KSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFG 274

Query: 269 YDGYGNIDFGDIGPSDQRETSLN---PHSSSYNVTIIQITVAGKPNNVEFTTIFDSGFSY 328
            DG G I FGD G  DQRET LN   PH  +YN+T+ +I+V G   ++EF  +FDSG S+
Sbjct: 275 NDGAGRISFGDKGSVDQRETPLNIRQPH-PTYNITVTKISVGGNTGDLEFDAVFDSGTSF 334

Query: 329 TYLSDPIYTLVTQNIDA-AMELDRAIFDSDYPFEYCYQLSLG-THFQQPHLNFTMDSGYI 388
           TYL+D  YTL++++ ++ A++      DS+ PFEYCY LS     FQ P +N TM  G  
Sbjct: 335 TYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVNLTMKGGSS 394

Query: 389 FGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCF 448
           + V    V IP  D      CL I K  DI++ G NFM G+R+VF+REK++LGW E +C+
Sbjct: 395 YPVYHPLVVIPMKDT--DVYCLAIMKIEDISIIGQNFMTGYRVVFDREKLILGWKESDCY 454

Query: 449 DN-------GADNSPPSNSPPADAPSPSGHSPPA--PSTTRTSNSTQLS 483
                     ++ S  S  PPA +  P   + P+  P+T+ TS +  LS
Sbjct: 455 TGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTSTTSAAYSLS 499

BLAST of CmaCh03G006590 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 358.6 bits (919), Expect = 8.3e-99
Identity = 206/491 (41.96%), Postives = 292/491 (59.47%), Query Frame = 0

Query: 11  MLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTP----GYYAAMVHRDRL 70
           +L+ LS     G + +  F +H RFSD +K   +S G   K  P     Y+ A+V RD L
Sbjct: 15  ILMLLSFGSCNGRIFT--FEMHHRFSDEVKQWSDSTGRFAKFPPKGSFEYFNALVLRDWL 74

Query: 71  LHARRL--ATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSW 130
           +  RRL  + S+SE+ LTFS  N T R   LG  +Y  + +GTP + F+VALDTGSDL W
Sbjct: 75  IRGRRLSESESESESSLTFSDGNSTSRISSLGFLHYTTVKLGTPGMRFMVALDTGSDLFW 134

Query: 131 LPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKI 190
           +PC+C KC  + G +   +  L+ Y+P  STT++ V C+NSLC   +QC    S+CPY +
Sbjct: 135 VPCDCGKCAPTEGATYASEFELSIYNPKVSTTNKKVTCNNSLCAQRNQCLGTFSTCPYMV 194

Query: 191 HDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAI-NGLLGLGM 250
              S  TS+SG L+ED++HL T D   + V+A +TFGCG+VQ+G F  IA  NGL GLGM
Sbjct: 195 SYVSAQTSTSGILMEDVMHLTTEDKNPERVEAYVTFGCGQVQSGSFLDIAAPNGLFGLGM 254

Query: 251 GKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRET--SLNPHSSSYNVTII 310
            KISVPS+LA + L  DSFSMCFG+DG G I FGD G SDQ ET  +LNP   +YN+T+ 
Sbjct: 255 EKISVPSVLAREGLVADSFSMCFGHDGVGRISFGDKGSSDQEETPFNLNPSHPNYNITVT 314

Query: 311 QITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQ 370
           ++ V     + EFT +FD+G S+TYL DP+YT V+++  +  +  R   DS  PFEYCY 
Sbjct: 315 RVRVGTTLIDDEFTALFDTGTSFTYLVDPMYTTVSESFHSQAQDKRHSPDSRIPFEYCYD 374

Query: 371 LSLGTHFQQ-PHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 430
           +S   +    P L+ TM     F +    + I T+  G    CL I KS+++N+ G N+M
Sbjct: 375 MSNDANASLIPSLSLTMKGNSHFTINDPIIVISTE--GELVYCLAIVKSSELNIIGQNYM 434

Query: 431 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPS--------GHSPPAPST 484
            G+R+VF+REK+VL W + +C+D    N+  + +    A +P+         +S     T
Sbjct: 435 TGYRVVFDREKLVLAWKKFDCYDIEETNTTVAGTNKTAAVAPAMAAGIKTHNNSSELHKT 494

BLAST of CmaCh03G006590 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-94
Identity = 218/501 (43.51%), Postives = 290/501 (57.88%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLL-EKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDN 88
           F +H  FSD +K  L  D L+ EK +  Y+  +  RDRL+  R LA++  ETP+TF   N
Sbjct: 31  FEVHHMFSDRVKQSLGLDDLVPEKGSLEYFKVLAQRDRLIRGRGLASNNEETPITFMRGN 90

Query: 89  HTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCEC-SKC---HTSVGTSDGED 148
            T     LG  +YAN+SVGTP   FLVALDTGSDL WLPC C S C      VG S    
Sbjct: 91  RTISIDLLGFLHYANVSVGTPATWFLVALDTGSDLFWLPCNCGSTCIRDLKEVGLSQSR- 150

Query: 149 ITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILH 208
             LN YSPN S+TS S+ CS+  C  + +C S  SSCPY+I   S +T ++G L ED+LH
Sbjct: 151 -PLNLYSPNTSSTSSSIRCSDDRCFGSSRCSSPASSCPYQIQYLSKDTFTTGTLFEDVLH 210

Query: 209 LATNDSQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQDLTPDSF 268
           L T D   +PV A IT GCGK QTG   SS A+NGLLGLG+   SVPS+LA   +T +SF
Sbjct: 211 LVTEDEGLEPVKANITLGCGKNQTGFLQSSAAVNGLLGLGLKDYSVPSILAKAKITANSF 270

Query: 269 SMCFG--YDGYGNIDFGDIGPSDQRETSLNP--HSSSYNVTIIQITVAGKPNNVEFTTIF 328
           SMCFG   D  G I FGD G +DQ ET L P   S +Y V++ +++V G    V+   +F
Sbjct: 271 SMCFGNIIDVVGRISFGDKGYTDQMETPLLPTEPSPTYAVSVTEVSVGGDAVGVQLLALF 330

Query: 329 DSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQLSLG-THFQQPHLNFTM 388
           D+G S+T+L +P Y L+T+  D  +   R   D + PFE+CY LS   T    P +  T 
Sbjct: 331 DTGTSFTHLLEPEYGLITKAFDDHVTDKRRPIDPELPFEFCYDLSPNKTTILFPRVAMTF 390

Query: 389 DSG-YIFGVVSMFVAIPTDDKGGHAICLTIAKSTD--INLFGLNFMAGHRIVFNREKMVL 448
           + G  +F    +F+    D+   +  CL I KS D  IN+ G NFM+G+RIVF+RE+M+L
Sbjct: 391 EGGSQMFLRNPLFIVWNEDNSAMY--CLGILKSVDFKINIIGQNFMSGYRIVFDRERMIL 450

Query: 449 GWNEVNCFDNGADNSPPSNSPPADAPSPSGHS-------PPAPSTTRTSNSTQLSPGIGT 508
           GW   +CF++ +  S     P  +APSPS  +       PPA +T    +    +   GT
Sbjct: 451 GWKRSDCFEDESLESTTPPPPETEAPSPSASTPLPSLLPPPAAATPPQIDPRNSTRNSGT 510

BLAST of CmaCh03G006590 vs. TAIR 10
Match: AT3G51340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-93
Identity = 214/503 (42.54%), Postives = 286/503 (56.86%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLL-EKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDN 88
           F +H  FSD +K  L  D L+ E  +  Y+  + HRDR +  R LA++  ETPLT    N
Sbjct: 32  FEVHHMFSDVVKQTLGFDDLVPENGSLEYFKVLAHRDRFIRGRGLASNNEETPLTSIGSN 91

Query: 89  HTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSK--CHTSVGTSDGEDIT 148
            T     LG  +YAN+S+GTP   FLVALDTGSDL WLPC C     H        E + 
Sbjct: 92  LTLALNFLGFLHYANVSLGTPATWFLVALDTGSDLFWLPCNCGTTCIHDLKDARFSESVP 151

Query: 149 LNHYSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLA 208
           LN Y+PNASTTS S+ CS+  C  + +C S +S CPY+I   S NT ++G L++D+LHL 
Sbjct: 152 LNLYTPNASTTSSSIRCSDKRCFGSGKCSSPESICPYQI-ALSSNTVTTGTLLQDVLHLV 211

Query: 209 TNDSQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQDLTPDSFSM 268
           T D   KPV+A +T GCG+ QTG F + IA+NG+LGL M + SVPSLLA  ++T +SFSM
Sbjct: 212 TEDEDLKPVNANVTLGCGQNQTGAFQTDIAVNGVLGLSMKEYSVPSLLAKANITANSFSM 271

Query: 269 CFG--YDGYGNIDFGDIGPSDQRETSL--NPHSSSYNVTIIQITVAGKPNNVEFTTIFDS 328
           CFG      G I FGD G +DQ ET L     S++Y V +  ++V G P +V    +FD+
Sbjct: 272 CFGRIISVVGRISFGDKGYTDQEETPLVSLETSTAYGVNVTGVSVGGVPVDVPLFALFDT 331

Query: 329 GFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQL--------SLGTHFQQPH 388
           G S+T L +  Y + T+  D  ME  R   D D+PFE+CY L        +   H Q   
Sbjct: 332 GSSFTLLLESAYGVFTKAFDDLMEDKRRPVDPDFPFEFCYDLREEHLNSDARPRHMQSKC 391

Query: 389 LNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFMAGHRIVFNREKM 448
            N   D            ++   ++G    CL I KS ++N+ G N M+GHRIVF+RE+M
Sbjct: 392 YNPCRDDFRWRIQNDSQESVSYSNEGTKMYCLGILKSINLNIIGQNLMSGHRIVFDRERM 451

Query: 449 VLGWNEVNCFDNG--ADNSPPSNSPPADAPSPSGHSPP-----APSTTRTSNSTQLSPGI 508
           +LGW + NCF++   A  SPP   P  +AP PS  +PP      P T    NST+ S   
Sbjct: 452 ILGWKQSNCFEDESLASESPP--PPEIEAPPPSVSTPPPAASATPPTIDPRNSTRNS--- 511

BLAST of CmaCh03G006590 vs. TAIR 10
Match: AT3G51350.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 335.5 bits (859), Expect = 7.5e-92
Identity = 215/503 (42.74%), Postives = 295/503 (58.65%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLL-EKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDN 88
           F +H  FSDS+K  L    L+ E+ +  Y+  + HRDRL+  R LA++  ETP+TF   N
Sbjct: 31  FEVHHIFSDSVKQSLGLGDLVPEQGSLEYFKVLAHRDRLIRGRGLASNNDETPITFDGGN 90

Query: 89  HTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCHTSVGTSDGEDI--- 148
            T     LG  YYAN+SVGTP   FLVALDTGSDL WLPC C     +    D EDI   
Sbjct: 91  LTVSVKLLGSLYYANVSVGTPPSSFLVALDTGSDLFWLPCNCG----TTCIRDLEDIGVP 150

Query: 149 ---TLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDI 208
               LN Y+PNASTTS S+ CS+  C  + +C S  S CPY+I   S +T + G L++D+
Sbjct: 151 QSVPLNLYTPNASTTSSSIRCSDKRCFGSKKCSSPSSICPYQI-SYSNSTGTKGTLLQDV 210

Query: 209 LHLATNDSQSKPVDAKITFGCGKVQTGIFS-SIAINGLLGLGMGKISVPSLLASQDLTPD 268
           LHLAT D    PV A +T GCG+ QTG+F  + ++NG+LGLG+   SVPSLLA  ++T +
Sbjct: 211 LHLATEDENLTPVKANVTLGCGQKQTGLFQRNNSVNGVLGLGIKGYSVPSLLAKANITAN 270

Query: 269 SFSMCFG--YDGYGNIDFGDIGPSDQRET---SLNPHSSSYNVTIIQITVAGKPNNVEFT 328
           SFSMCFG      G I FGD G +DQ ET   S+ P S++Y V I  ++VAG P ++   
Sbjct: 271 SFSMCFGRVIGNVGRISFGDRGYTDQEETPFISVAP-STAYGVNISGVSVAGDPVDIRLF 330

Query: 329 TIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCYQLS-LGTHFQQPHLN 388
             FD+G S+T+L +P Y ++T++ D  +E  R   D + PFE+CY LS   T  Q P + 
Sbjct: 331 AKFDTGSSFTHLREPAYGVLTKSFDELVEDRRRPVDPELPFEFCYDLSPNATTIQFPLVE 390

Query: 389 FTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKST--DINLFGLNFMAGHRIVFNREKM 448
            T   G    + + F    T + G    CL + KS    IN+ G NF+AG+RIVF+RE+M
Sbjct: 391 MTFIGGSKIILNNPFFTARTQE-GNVMYCLGVLKSVGLKINVIGQNFVAGYRIVFDRERM 450

Query: 449 VLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNST--QLSP-----GI 508
           +LGW +  CF++ +  S     P  +AP+PS  +PP  S   T ++T   ++P       
Sbjct: 451 ILGWKQSLCFEDESLESTTPPPPEVEAPAPSVSAPPPRSLPPTVSATPPPINPRNSTGNP 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYV91.6e-11046.91Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Q9LX203.5e-6235.99Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ... [more]
Q4V3D24.1e-2625.21Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Q9S9K41.6e-2526.59Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q9M9A86.7e-2127.03Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IJH67.6e-294100.00aspartyl protease family protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A6J1GFR65.0e-28597.25aspartyl protease family protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1BTF71.3e-17963.07aspartyl protease family protein 1-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KGS01.1e-17864.34Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G50724... [more]
A0A1S3B2V81.0e-17663.83aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485160 ... [more]
Match NameE-valueIdentityDescription
XP_022977812.11.6e-293100.00aspartyl protease family protein 1-like [Cucurbita maxima][more]
XP_022950419.11.0e-28497.25aspartyl protease family protein 1-like [Cucurbita moschata][more]
XP_023521822.13.5e-27794.51aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_023544616.18.7e-26090.59aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo][more]
KAG6603890.19.4e-25492.40Peroxidase 64, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G17760.11.1e-11146.91Eukaryotic aspartyl protease family protein [more]
AT4G35880.18.3e-9941.96Eukaryotic aspartyl protease family protein [more]
AT3G51330.12.1e-9443.51Eukaryotic aspartyl protease family protein [more]
AT3G51340.11.8e-9342.54Eukaryotic aspartyl protease family protein [more]
AT3G51350.17.5e-9242.74Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 99..278
e-value: 2.5E-35
score: 122.3
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 312..436
e-value: 1.0E-10
score: 41.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 282..447
e-value: 6.0E-24
score: 86.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 72..278
e-value: 6.1E-40
score: 139.2
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 96..442
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 453..467
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 445..486
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 468..486
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 8..454
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 8..454
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 114..125
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 99..437
score: 31.343945

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G006590.1CmaCh03G006590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016020 membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity