CmaCh03G006520 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G006520
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cma_Chr03: 5513269 .. 5515554 (+)
RNA-Seq Expression	CmaCh03G006520
Synteny	CmaCh03G006520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTTAATTACGGTGTCAAGATTCACAGTTTGTCTGTACAGAGACCTTTGGACTTTGGGGACGAAGACCAAAAGCTTGGAATTCCAGATCTTGCTCCCCAAGGGTTTTGTTTCCATGGCGAAGCACAGGCAATCTCGTTTTCCTACTCGGAAGTCGTCTTCTTCATCTACTATCATTTTTACCCTGCTCATTATGTTCACCTTCGTCATTCTGATTCTTCTTGCTCTTGGAATCCTCTCGATCCCTGGAAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGCTCGATTGTTCGGAAAACCTCTGAGGAGTATGTTATCTTATGGATTTTCTTTCGATTTTTTGTTCTGTATGAGGATGGATTTTGATTGATTCTGTGGTTTTGTTTGATTTTGTTTCAGTGTTGATGAGGAGAAAGGGGAACGGTGGGCTGAAGTTATCTCATGGGAACCTAGAGCCTTTGTTTATCACAACTTTCTGGTATTTCAGTTTTCCCGATCTTGATTGAGAGATTTGTGATTTATGTTCATTCATCTCTCTCCTAATGCAATTTGTGTATGGAATTCTTCAATAAACTTGGTTGGTGCATGCTTATACACGTTGAATGCGTCTTTGGTGCTGCACCTTCTTTAAGCTTATTATGTTTATGTTCCTCTAAGAAATCATTCTTCGTTCTTATCATGATGAGTCAAGGATACTTCCCATTGTGTTTGTCTTTAGATGGTATTGGAGTATGAGTTGACTGAGGCTGAGACCCATCGATTAATCCCGGAGTAGGGAAATACTGAATTGGTTTACTTTTGAATTGCTCAGACAAAGGAGGAGTGTGAGTACCTAATTAGCCATGCCAAGCCTCATATGCAAAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGGTGTTCTATTCCTTTCCTCAGAATTCTTTACTGTTAGTAATGCTGGATCTCTTTTGTGGAAACCTCTCTTACCTCTCTTTCACAGAGTTCGCACTAGCTCCGGAACATTCTTGCCGAGAGGAGGCGATAAGATTATCAGCACTATAGAGAAAAGGATTGCTGATTTCACCTTCATACCCATAGGTACTATTTTTTAGTCCATTTCCTCCTGGTTTCTTCCAACTACATCGAGCATTATTTTCTATTATGCAAGGTTAAAATAATCTTTTGTAATCTTTAATTTATTTTCAAGTGCACCTATCGTTTTGGTATAATTTTCCCCCTGTAGTCTAGCTTTCCCAATAACTTTTCTTTTGTTAGAAAAGATTTTTATCATGCCCAAAACTACTCGAATAGATACAATTATCGTGCTAAGTTTCAATGGATGAGGAAGATTTTTAATCATGGCTGAAAATTTGAATAACGAATATCAACTCAGACGTAGGAATACCAATACATTCCAGATATTTGTTTGTCCTATGAGCTTACCTGCCCATTGTCCCTTGTTAGAATGAACGAGAATCGGGTTCACCTGATAAACTGTGCCAATTTTTGTTTATTATATGTAGATATTGTCTTCTTATCTTTTAAAATTTTCATATGGTCAATGAATATTAGACACTAAGTTTTCGTACTTTGTTACTTAAGTGCACACATAATATATGCAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGACGACTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTACCTGTAAGTAGTAATCCATATTTTCCATGCTTTGGACTCTAAACAAGTACTTCATCTTGCATGGTTCGCCCATTATTTTATATGAATTCTATCGTAATTCAGCTCAGACGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTGCCTTGGTGGAATGAGCTTTCTGATTGTGGGAAGAAAGGACTTTCCGTTAAACCGAAGAGGGGCGATGCATTGCTTTTCTGGAGCATGAAGCCCGATGCCTCTCTAGACCCTTCAAGTTTGCATGGTTAGATTAGATACTCTCAAACTTATAAACCAACCATTCATTTTTCTGAGCATGTTAGATTTCAGTGATGTATGTTTCGTTCGTTTTGTCAGTGCGCTTTCTTTTTCTTCTCTTATGCGGAGAGTATCGGATTTTCTTCATAAAATGACGATATTTCTCCTGCTGTTCATATATAGGTGGCTGTCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGATGAATACAAAGCTTGA

mRNA sequence

TCTTTAATTACGGTGTCAAGATTCACAGTTTGTCTGTACAGAGACCTTTGGACTTTGGGGACGAAGACCAAAAGCTTGGAATTCCAGATCTTGCTCCCCAAGGGTTTTGTTTCCATGGCGAAGCACAGGCAATCTCGTTTTCCTACTCGGAAGTCGTCTTCTTCATCTACTATCATTTTTACCCTGCTCATTATGTTCACCTTCGTCATTCTGATTCTTCTTGCTCTTGGAATCCTCTCGATCCCTGGAAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGCTCGATTGTTCGGAAAACCTCTGAGGATGTTGATGAGGAGAAAGGGGAACGGTGGGCTGAAGTTATCTCATGGGAACCTAGAGCCTTTGTTTATCACAACTTTCTGACAAAGGAGGAGTGTGAGTACCTAATTAGCCATGCCAAGCCTCATATGCAAAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACTAGCTCCGGAACATTCTTGCCGAGAGGAGGCGATAAGATTATCAGCACTATAGAGAAAAGGATTGCTGATTTCACCTTCATACCCATAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGACGACTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTACCTCTCAGACGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTGCCTTGGTGGAATGAGCTTTCTGATTGTGGGAAGAAAGGACTTTCCGTTAAACCGAAGAGGGGCGATGCATTGCTTTTCTGGAGCATGAAGCCCGATGCCTCTCTAGACCCTTCAAGTTTGCATGGTGGCTGTCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGATGAATACAAAGCTTGA

Coding sequence (CDS)

ATGGCGAAGCACAGGCAATCTCGTTTTCCTACTCGGAAGTCGTCTTCTTCATCTACTATCATTTTTACCCTGCTCATTATGTTCACCTTCGTCATTCTGATTCTTCTTGCTCTTGGAATCCTCTCGATCCCTGGAAATTCCGGCGGTTCGCCCAAGGTTCATGATCTGAGCTCGATTGTTCGGAAAACCTCTGAGGATGTTGATGAGGAGAAAGGGGAACGGTGGGCTGAAGTTATCTCATGGGAACCTAGAGCCTTTGTTTATCACAACTTTCTGACAAAGGAGGAGTGTGAGTACCTAATTAGCCATGCCAAGCCTCATATGCAAAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACTAGCTCCGGAACATTCTTGCCGAGAGGAGGCGATAAGATTATCAGCACTATAGAGAAAAGGATTGCTGATTTCACCTTCATACCCATAGAGCATGGAGAAGGACTTCAGGTTCTTCATTACGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGACGACTACAATACAAAGAATGGAGGTCAACGTATAGCAACAGTGCTTATGTACCTCTCAGACGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTGCCTTGGTGGAATGAGCTTTCTGATTGTGGGAAGAAAGGACTTTCCGTTAAACCGAAGAGGGGCGATGCATTGCTTTTCTGGAGCATGAAGCCCGATGCCTCTCTAGACCCTTCAAGTTTGCATGGTGGCTGTCCTGTTATCAAGGGGAATAAATGGTCTGCTACTAAATGGATGCGAGTAGATGAATACAAAGCTTGA

Protein sequence

MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA

Homology

[Close]

BLAST of CmaCh03G006520 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 7.2e-129
Identity = 222/288 (77.08%), Postives = 251/288 (87.15%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MA+ R  R P+ + SS ST++F +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKTSE--DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSET 120
           RKT +    D+ K ERW E+ISWEPRA VYHNFLTKEEC+YLI  AKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RG DK I  IEKRI+DFTFIP+EHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+D+YNT+NGGQRIATVLMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of CmaCh03G006520 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.4e-119
Identity = 211/288 (73.26%), Postives = 245/288 (85.07%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAK R SRF  RK S+   ++F +L M T V+L+LLA G+ S+P N+  S  + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSEDVD--EEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSET 120
           R  +E  +   ++G++W EV+SWEPRAFVYHNFL+KEECEYLIS AKPHM KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHF 180
           GKSKDSRVRTSSGTFL RG DKII TIEKRIAD+TFIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+D++NTKNGGQR+AT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELS+CGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287
           KP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of CmaCh03G006520 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 5.1e-119
Identity = 207/281 (73.67%), Postives = 239/281 (85.05%), Query Frame = 0


Query: 8   RFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK--TSE 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGKSKDSR 127
             +E  GERW EVISWEPRA VYHNFLT EECE+LIS AKP M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDYFLDDY 187
           VRTSSGTFL RG D+++  IEKRI+DFTFIP+E+GEGLQVLHY+VGQKYEPH+DYFLD++
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDA 247
           NTKNGGQRIATVLMYLSDV++GGETVFPAA+GN S+VPWWNELS CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of CmaCh03G006520 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 3.0e-111
Identity = 200/288 (69.44%), Postives = 230/288 (79.86%), Query Frame = 0


Query: 3   KHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV+ 
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  TSE-----DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSE 122
             E     D ++  G+RW EVISWEPRAFVYHNFLT EECE+LIS AKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+I+  IE RI+DFTFIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLS 242
            DYF D++N + GGQRIATVLMYLSDV+EGGETVFPAAKGN S VPWW+ELS CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEY 286
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of CmaCh03G006520 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-65
Identity = 123/231 (53.25%), Postives = 170/231 (73.59%), Query Frame = 0


Query: 57  SSIVRKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDS 116
           +S++  +S  V+  K     + +S +PRAFVY  FLT+ EC++++S AK  +++S V D+
Sbjct: 22  TSLISSSSVFVNPSK----VKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADN 81

Query: 117 ETGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEP 176
           ++G+SK S VRTSSGTF+ +G D I+S IE +I+ +TF+P E+GE +QVL YE GQKY+ 
Sbjct: 82  DSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDA 141

Query: 177 HFDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWN--ELSDCGKK 236
           HFDYF D  N   GG R+AT+LMYLS+V +GGETVFP A+     V   N  +LSDC K+
Sbjct: 142 HFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKR 201

Query: 237 GLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEY 286
           G++VKP++GDALLF+++ PDA  DP SLHGGCPVI+G KWSATKW+ VD +
Sbjct: 202 GIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSF 248

[Close]

BLAST of CmaCh03G006520 vs. ExPASy TrEMBL
Match: A0A6J1IRF1 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.4e-164
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. ExPASy TrEMBL
Match: A0A6J1GDV6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.1e-163
Identity = 285/287 (99.30%), Postives = 286/287 (99.65%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSR PTRKSSSSST+IFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRLPTRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. ExPASy TrEMBL
Match: A0A1S3B1P6 (probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.5e-156
Identity = 270/287 (94.08%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTS+DVDEEKGE+W EVISWEPRAF+YHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK I TIEKRI+DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. ExPASy TrEMBL
Match: A0A0A0KJH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507320 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.3e-156
Identity = 269/287 (93.73%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK + TIEKR++DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. ExPASy TrEMBL
Match: A0A6J1EFG6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.6e-152
Identity = 266/287 (92.68%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQ RFP+RKSSSSST+IFTLLIMFTFVILILLALGILSIPGNS G+PK HDLSSIV
Sbjct: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTS++VDEEKGE+WAEVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKS+VVDSETGK
Sbjct: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DKII  IEKRIADF+F+P+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of CmaCh03G006520 vs. NCBI nr
Match: XP_022978860.1 (probable prolyl 4-hydroxylase 10 [Cucurbita maxima])

HSP 1 Score: 587.8 bits (1514), Expect = 5.1e-164
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. NCBI nr
Match: XP_022950101.1 (probable prolyl 4-hydroxylase 10 [Cucurbita moschata])

HSP 1 Score: 584.7 bits (1506), Expect = 4.3e-163
Identity = 285/287 (99.30%), Postives = 286/287 (99.65%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSR PTRKSSSSST+IFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRLPTRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. NCBI nr
Match: XP_023521904.1 (probable prolyl 4-hydroxylase 10 [Cucurbita pepo subsp. pepo] >XP_023543233.1 probable prolyl 4-hydroxylase 10 [Cucurbita pepo subsp. pepo] >KAG6603884.1 putative prolyl 4-hydroxylase 10, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034063.1 putative prolyl 4-hydroxylase 10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584.3 bits (1505), Expect = 5.6e-163
Identity = 284/287 (98.95%), Postives = 286/287 (99.65%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSR PTRKSSSSST+IFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV
Sbjct: 1   MAKHRQSRLPTRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. NCBI nr
Match: XP_008440878.1 (PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 270/287 (94.08%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTS+DVDEEKGE+W EVISWEPRAF+YHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK I TIEKRI+DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. NCBI nr
Match: XP_004134841.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prolyl 4-hydroxylase 10 [Cucumis sativus] >KGN48959.1 hypothetical protein Csa_003630 [Cucumis sativus])

HSP 1 Score: 559.3 bits (1440), Expect = 1.9e-155
Identity = 269/287 (93.73%), Postives = 281/287 (97.91%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAKHRQSRFPTRKSSSSST++FTLLIMFTFVILILLALGILSIPGNSGGS KVHDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120
           RKTS+DVDEEKGE+W EVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK + TIEKR++DF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 288
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of CmaCh03G006520 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 5.1e-130
Identity = 222/288 (77.08%), Postives = 251/288 (87.15%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MA+ R  R P+ + SS ST++F +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKTSE--DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSET 120
           RKT +    D+ K ERW E+ISWEPRA VYHNFLTKEEC+YLI  AKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RG DK I  IEKRI+DFTFIP+EHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+D+YNT+NGGQRIATVLMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of CmaCh03G006520 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 430.6 bits (1106), Expect = 9.6e-121
Identity = 211/288 (73.26%), Postives = 245/288 (85.07%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MAK R SRF  RK S+   ++F +L M T V+L+LLA G+ S+P N+  S  + DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSEDVD--EEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSET 120
           R  +E  +   ++G++W EV+SWEPRAFVYHNFL+KEECEYLIS AKPHM KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHF 180
           GKSKDSRVRTSSGTFL RG DKII TIEKRIAD+TFIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSV 240
           DYF+D++NTKNGGQR+AT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELS+CGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287
           KP+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of CmaCh03G006520 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 428.7 bits (1101), Expect = 3.7e-120
Identity = 207/281 (73.67%), Postives = 239/281 (85.05%), Query Frame = 0


Query: 8   RFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK--TSE 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  +  S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGKSKDSR 127
             +E  GERW EVISWEPRA VYHNFLT EECE+LIS AKP M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDYFLDDY 187
           VRTSSGTFL RG D+++  IEKRI+DFTFIP+E+GEGLQVLHY+VGQKYEPH+DYFLD++
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKPKRGDA 247
           NTKNGGQRIATVLMYLSDV++GGETVFPAA+GN S+VPWWNELS CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of CmaCh03G006520 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 2.1e-112
Identity = 200/288 (69.44%), Postives = 230/288 (79.86%), Query Frame = 0


Query: 3   KHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIVRK 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV+ 
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  TSE-----DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSE 122
             E     D ++  G+RW EVISWEPRAFVYHNFLT EECE+LIS AKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+I+  IE RI+DFTFIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLS 242
            DYF D++N + GGQRIATVLMYLSDV+EGGETVFPAAKGN S VPWW+ELS CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEY 286
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of CmaCh03G006520 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 8.8e-98
Identity = 176/237 (74.26%), Postives = 202/237 (85.23%), Query Frame = 0


Query: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60
           MA+ R  R P+ + SS ST++F +LIM TFVILILLA GILS+P N+ GS K +DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKTSE--DVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSET 120
           RKT +    D+ K ERW E+ISWEPRA VYHNFL  EEC+YLI  AKPHM+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RG DK I  IEKRI+DFTFIP+EHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKG 236
           DYF+D+YNT+NGGQRIATVLMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGK G
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:

BLAST of CmaCh03G006520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JZ24	7.2e-129	77.08	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q9LN20	1.4e-119	73.26	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
Q24JN5	5.1e-119	73.67	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
F4JNU8	3.0e-111	69.44	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q8LAN3	1.5e-65	53.25	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of CmaCh03G006520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IRF1	2.4e-164	100.00	probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=...	[more]
A0A6J1GDV6	2.1e-163	99.30	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111453285 P...	[more]
A0A1S3B1P6	5.5e-156	94.08	probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV...	[more]
A0A0A0KJH9	9.3e-156	93.73	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1EFG6	1.6e-152	92.68	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 P...	[more]

BLAST of CmaCh03G006520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022978860.1	5.1e-164	100.00	probable prolyl 4-hydroxylase 10 [Cucurbita maxima]	[more]
XP_022950101.1	4.3e-163	99.30	probable prolyl 4-hydroxylase 10 [Cucurbita moschata]	[more]
XP_023521904.1	5.6e-163	98.95	probable prolyl 4-hydroxylase 10 [Cucurbita pepo subsp. pepo] >XP_023543233.1 pr...	[more]
XP_008440878.1	1.1e-155	94.08	PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo]	[more]
XP_004134841.1	1.9e-155	93.73	probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prol...	[more]

BLAST of CmaCh03G006520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66060.1	5.1e-130	77.08	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	9.6e-121	73.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	3.7e-120	73.67	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	2.1e-112	69.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	8.8e-98	74.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 83..281 e-value: 5.0E-67 score: 238.7
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 76..282 e-value: 5.3E-82 score: 276.5
None	No IPR available	PANTHER	PTHR10869:SF157	PROLYL 4-HYDROXYLASE 10-RELATED	coord: 1..286
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 163..281 e-value: 5.8E-22 score: 78.4
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..286
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 159..282 score: 12.68751

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G006520.1	CmaCh03G006520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen