CmaCh03G006500 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G006500
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRNA-binding (RRM/RBD/RNP motifs) family protein
LocationCma_Chr03: 5502224 .. 5504523 (-)
RNA-Seq ExpressionCmaCh03G006500
SyntenyCmaCh03G006500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACTCTCTCTCCTCGCACAAGTCACGACGGCGAGTTGCGATTCTGGGCTCGTGGAGAGGCCGTCTTCAGAAACCCTAAAAGTCCTGTTCGTATCTGTACTGTTCTTCAGCTTTTGCTGGCCGCCGTTCTAGTTTTCATCTCATTGTGAAAAATGGTAAGCTCTTCTTTCCCAGTTCTATTTTTTCTTTCTTTGTCTATTGTTGCTTTTATTTCATCGATAAAAGGTTTAACAATCGAGCGTTTCTGTTTCTGCGCAGGCCGATTCTCATCGCAAAAGGTGAGCTTATTTACGCTTTTTTGTAATGATTTGTTTTCTTACGATTGACGATCTTGGGGCTTTCTATATCTAGGGCACGGAGATGATTTGTGTTATAGCATTCATGATCTTCTGTAGCGTTGAACTTTTTAGCACTTTTTATTATGAGTTGTCGATTAACTAAATTTTAATTCTACATAAAGGAAATACTTGTCACGAGTCTTGAGCATTTTTAGAAGGATTTTATTTCATTCATTGAGACGCCTGTAACTAGGGTTGAGCAAATTTTCTTGACAATGGTGGCTGTAATCACTTTAGGGTTTTGGAAAAAGTAACAAGGTCATGCATTACTTTTCTTTTTCTTTGTCTTAGGAAGTTCTAGCAGGTAGTAATCAAGAAATCAAATTAAAAGCAATTATACTGGGTAACTATTGTTGTAATTAATGGAGTTTGAGAGATTTGAGTATCCAAATTTTGAAGTTAATATAGGGTCTGAATTGGGTCAAGTGCCATGCGAAAGAACTTTAGTCGCTTGAACTCGCACCCTTTGTTTTCATTTTAAAACTTGGCTGTTGATGATTGAGCTGGGAATAATTTCTGATGATTTCCCTTCTCTTGTTCTGTTGGTTTCCTTTTTCCATTTTGACCATATTAAGTTGTCAGACTTGAAAACTCGTTCTGTCAGTAATTTACACATTAAGGATCAACAGAAACATGGGTGGAAAAGATTATGTGCGTTTACTATATTATAAAACTTGGCAGTTTGATAATTTAGACCATTCCTTTCTTTTAGAACATCCTCATTTTAATAATTCTAATGCATTTAGGTCGTCGGTTTTAGTACTACTCACGGTTGTAAGTGGCACAGGTACTCTCGATCTCCTTCTCCCTGGAGAGCTCCTTCAAGATCAAGATCTAGATCACGATCTAGGCCAAGGTCTAGATCCAGATCGTGGTCAAGGCCAAGGTCCCGAAGTCGTGGAAGGTTGGTTTTGATTTTTCTTTATTACCTGGATAAATGGAAAGTAGAAACTACTTTTATGTTTGTGAACTCTGTAGCTTAGAACCCTATTTTTATGGTTACACATGTTTCTGATACAGATCAAGATCCAGAAGTCGAGGAAGGTGTGTGTGTTTCTTCGGCTGGAACTCTAGACAAAATAATCAGCGCTCTATTTCAGTAGAATTGGATCTATTTGTACTTCTATTTCATATATTGTTAGTGTTCGATTCTCCTTGGATAACATTTTTAGCTTCTTCCAGGGGGGAGAATGTCAATAACCCTGGGAATACGCTTTATGTCACTGGCCTATCTACAAGGGTCACTGAAAGAGACCTTGAAGAACACTTTTCCAAGGAGGGCAAGGTTAGCTTCTGAACCCTATAACTTGCAGATAATTGCATGATCGTGTGAGAAGTCCCTACTTGGATCATGATGTGAAACAAGTGGCATGACATAAAGAAGACCGTATTTATTGTGTTTCTTGAAATTAAAAATGTTTTTATAGTATTTCCTGAATTGAAAAACATTTAAATGGAAATTGGGTAGATGACAGATGGTCTCAAGGTCAGTGTATTCATAATATCCCACTGTATTGAAAAACAGAAATTGATTTGACTCCAAATGGCCCGATGTTCTGCAATTACTTGTTTTATTGATCACGATGTAGTCAGTTTATTCTTCCTTTCCTAATGTTTCTATTATTTGCAGGTATCTTCTTGTTTCCTTGTGGTTGAACCTCGAACTCGGATTTCCCGTGGTTTTGCATTTGTAACCATGGACAATGTTGATGATGCCAATCGGTGTGTTAAGCATCTCAACCAGTCAATTCTAGAAGGTCGATATATAACTGTGGAGAAGGTACTACGATTGTTCTGCCTTGGATAGCCTTGAAGAAATTTGATTGTTTTCTTTTTTGGTTTTTTCCCTTAACTTGGAGGTTTTCTGGTTATATTCAGAACAAGTTGTGTGAGCTATGTTGGCAGCAATCAAAGATGATGAGTTCATCCATGTCAACTTTAGTGAAACAGCTTGGTCAATGA

mRNA sequence

AAACTCTCTCTCCTCGCACAAGTCACGACGGCGAGTTGCGATTCTGGGCTCGTGGAGAGGCCGTCTTCAGAAACCCTAAAAGTCCTGTTCGTATCTGTACTGTTCTTCAGCTTTTGCTGGCCGCCGTTCTAGTTTTCATCTCATTGTGAAAAATGGCCGATTCTCATCGCAAAAGGTACTCTCGATCTCCTTCTCCCTGGAGAGCTCCTTCAAGATCAAGATCTAGATCACGATCTAGGCCAAGGTCTAGATCCAGATCGTGGTCAAGGCCAAGGTCCCGAAGTCGTGGAAGATCAAGATCCAGAAGTCGAGGAAGGGGGGAGAATGTCAATAACCCTGGGAATACGCTTTATGTCACTGGCCTATCTACAAGGGTCACTGAAAGAGACCTTGAAGAACACTTTTCCAAGGAGGGCAAGGTATCTTCTTGTTTCCTTGTGGTTGAACCTCGAACTCGGATTTCCCGTGGTTTTGCATTTGTAACCATGGACAATGTTGATGATGCCAATCGGTGTGTTAAGCATCTCAACCAGTCAATTCTAGAAGGTCGATATATAACTGTGGAGAAGAACAAGTTGTGTGAGCTATGTTGGCAGCAATCAAAGATGATGAGTTCATCCATGTCAACTTTAGTGAAACAGCTTGGTCAATGA

Coding sequence (CDS)

ATGGCCGATTCTCATCGCAAAAGGTACTCTCGATCTCCTTCTCCCTGGAGAGCTCCTTCAAGATCAAGATCTAGATCACGATCTAGGCCAAGGTCTAGATCCAGATCGTGGTCAAGGCCAAGGTCCCGAAGTCGTGGAAGATCAAGATCCAGAAGTCGAGGAAGGGGGGAGAATGTCAATAACCCTGGGAATACGCTTTATGTCACTGGCCTATCTACAAGGGTCACTGAAAGAGACCTTGAAGAACACTTTTCCAAGGAGGGCAAGGTATCTTCTTGTTTCCTTGTGGTTGAACCTCGAACTCGGATTTCCCGTGGTTTTGCATTTGTAACCATGGACAATGTTGATGATGCCAATCGGTGTGTTAAGCATCTCAACCAGTCAATTCTAGAAGGTCGATATATAACTGTGGAGAAGAACAAGTTGTGTGAGCTATGTTGGCAGCAATCAAAGATGATGAGTTCATCCATGTCAACTTTAGTGAAACAGCTTGGTCAATGA

Protein sequence

MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRYITVEKNKLCELCWQQSKMMSSSMSTLVKQLGQ
Homology
BLAST of CmaCh03G006500 vs. ExPASy Swiss-Prot
Match: Q84TH4 (Serine/arginine-rich splicing factor SR45a OS=Arabidopsis thaliana OX=3702 GN=SR45A PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.5e-25
Identity = 68/123 (55.28%), Postives = 85/123 (69.11%), Query Frame = 0

Query: 17  RAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVNNPGNTLYVTGLSTRVT 76
           R+P     RSR  P        R RS SR  SRS +R    +  NPGN+LYVTGLS RVT
Sbjct: 28  RSPMSYSRRSRYSPSLSPYDKRRGRSVSRSLSRSPTRSVSSDAENPGNSLYVTGLSHRVT 87

Query: 77  ERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRYIT 136
           ERDLE+HF+KEGKV+   LV++P TR SRGF F++M +V DANRC++ L+ S+L+GR IT
Sbjct: 88  ERDLEDHFAKEGKVTDVHLVLDPWTRESRGFGFISMKSVGDANRCIRSLDHSVLQGRVIT 147

Query: 137 VEK 140
           VEK
Sbjct: 148 VEK 150

BLAST of CmaCh03G006500 vs. ExPASy Swiss-Prot
Match: Q3ZBT6 (Transformer-2 protein homolog beta OS=Bos taurus OX=9913 GN=TRA2B PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-12
Identity = 68/151 (45.03%), Postives = 81/151 (53.64%), Query Frame = 0

Query: 3   DSHRKR-YSRSPSPWRAPS---------RSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRS 62
           DS R R  SRS S  R+ S         RSRSRSRS  RSRSRS+SR   R    S S  
Sbjct: 42  DSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPM 101

Query: 63  RGRGENVNN-----PGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGF 122
             R  +V N     P   L V GLS   TERDL E FSK G ++   +V + ++R SRGF
Sbjct: 102 STRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGF 161

Query: 123 AFVTMDNVDDANRCVKHLNQSILEGRYITVE 139
           AFV  +NVDDA    +  N   L+GR I V+
Sbjct: 162 AFVYFENVDDAKEAKERANGMELDGRRIRVD 192

BLAST of CmaCh03G006500 vs. ExPASy Swiss-Prot
Match: P62995 (Transformer-2 protein homolog beta OS=Homo sapiens OX=9606 GN=TRA2B PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-12
Identity = 68/151 (45.03%), Postives = 81/151 (53.64%), Query Frame = 0

Query: 3   DSHRKR-YSRSPSPWRAPS---------RSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRS 62
           DS R R  SRS S  R+ S         RSRSRSRS  RSRSRS+SR   R    S S  
Sbjct: 42  DSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPM 101

Query: 63  RGRGENVNN-----PGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGF 122
             R  +V N     P   L V GLS   TERDL E FSK G ++   +V + ++R SRGF
Sbjct: 102 STRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGF 161

Query: 123 AFVTMDNVDDANRCVKHLNQSILEGRYITVE 139
           AFV  +NVDDA    +  N   L+GR I V+
Sbjct: 162 AFVYFENVDDAKEAKERANGMELDGRRIRVD 192

BLAST of CmaCh03G006500 vs. ExPASy Swiss-Prot
Match: P62996 (Transformer-2 protein homolog beta OS=Mus musculus OX=10090 GN=Tra2b PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-12
Identity = 68/151 (45.03%), Postives = 81/151 (53.64%), Query Frame = 0

Query: 3   DSHRKR-YSRSPSPWRAPS---------RSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRS 62
           DS R R  SRS S  R+ S         RSRSRSRS  RSRSRS+SR   R    S S  
Sbjct: 42  DSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPM 101

Query: 63  RGRGENVNN-----PGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGF 122
             R  +V N     P   L V GLS   TERDL E FSK G ++   +V + ++R SRGF
Sbjct: 102 STRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGF 161

Query: 123 AFVTMDNVDDANRCVKHLNQSILEGRYITVE 139
           AFV  +NVDDA    +  N   L+GR I V+
Sbjct: 162 AFVYFENVDDAKEAKERANGMELDGRRIRVD 192

BLAST of CmaCh03G006500 vs. ExPASy Swiss-Prot
Match: P62997 (Transformer-2 protein homolog beta OS=Rattus norvegicus OX=10116 GN=Tra2b PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-12
Identity = 68/151 (45.03%), Postives = 81/151 (53.64%), Query Frame = 0

Query: 3   DSHRKR-YSRSPSPWRAPS---------RSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRS 62
           DS R R  SRS S  R+ S         RSRSRSRS  RSRSRS+SR   R    S S  
Sbjct: 42  DSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPM 101

Query: 63  RGRGENVNN-----PGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGF 122
             R  +V N     P   L V GLS   TERDL E FSK G ++   +V + ++R SRGF
Sbjct: 102 STRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGF 161

Query: 123 AFVTMDNVDDANRCVKHLNQSILEGRYITVE 139
           AFV  +NVDDA    +  N   L+GR I V+
Sbjct: 162 AFVYFENVDDAKEAKERANGMELDGRRIRVD 192

BLAST of CmaCh03G006500 vs. ExPASy TrEMBL
Match: A0A6J1INV2 (serine/arginine-rich splicing factor SR45a OS=Cucurbita maxima OX=3661 GN=LOC111478126 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.7e-67
Identity = 139/141 (98.58%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN 60
           MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN
Sbjct: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN 60

Query: 61  NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR 120
           NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR
Sbjct: 61  NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR 120

Query: 121 CVKHLNQSILEGRYITVEKNK 142
           CVKHLNQSILEGRYITVEK++
Sbjct: 121 CVKHLNQSILEGRYITVEKSR 141

BLAST of CmaCh03G006500 vs. ExPASy TrEMBL
Match: A0A6J1GFF0 (serine/arginine-rich splicing factor SR45a OS=Cucurbita moschata OX=3662 GN=LOC111453689 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.7e-67
Identity = 139/141 (98.58%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN 60
           MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN
Sbjct: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN 60

Query: 61  NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR 120
           NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR
Sbjct: 61  NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR 120

Query: 121 CVKHLNQSILEGRYITVEKNK 142
           CVKHLNQSILEGRYITVEK++
Sbjct: 121 CVKHLNQSILEGRYITVEKSR 141

BLAST of CmaCh03G006500 vs. ExPASy TrEMBL
Match: A0A0A0KMM4 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507340 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.5e-60
Identity = 132/143 (92.31%), Postives = 137/143 (95.80%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAP--SRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGEN 60
           MAD HRKRYSRSPSPW+AP  SRSRSRSRSRPRSRSRSWSRPRS SRGRSRSRSRGR E+
Sbjct: 1   MADVHRKRYSRSPSPWKAPSRSRSRSRSRSRPRSRSRSWSRPRSPSRGRSRSRSRGR-EH 60

Query: 61  VNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDA 120
            NNPGNTLYVTGLSTRVTERDLEEHFSKEGKV+SCFLVVEPRTRISRGFAFVTMDNVDDA
Sbjct: 61  ANNPGNTLYVTGLSTRVTERDLEEHFSKEGKVASCFLVVEPRTRISRGFAFVTMDNVDDA 120

Query: 121 NRCVKHLNQSILEGRYITVEKNK 142
           NRCVKHLNQSILEGRYITVEK++
Sbjct: 121 NRCVKHLNQSILEGRYITVEKSR 142

BLAST of CmaCh03G006500 vs. ExPASy TrEMBL
Match: A0A251ND00 (RRM domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_7G175500 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.5e-60
Identity = 136/170 (80.00%), Postives = 149/170 (87.65%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPR----SRSRGRSRSRSRGRG 60
           MADS RK+YSRSPSPWRA SRSRSRS SRPRSRSRSWSRPR    SRSRGRSRSRS GR 
Sbjct: 1   MADSPRKKYSRSPSPWRAQSRSRSRSWSRPRSRSRSWSRPRQSSHSRSRGRSRSRSPGRP 60

Query: 61  ENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVD 120
             + NPGNTLYVTGLSTRV+E+D+E HFSKEGKV+SCFLVVEPRTRISRGFAFVTMD+V+
Sbjct: 61  AAI-NPGNTLYVTGLSTRVSEKDVERHFSKEGKVASCFLVVEPRTRISRGFAFVTMDSVE 120

Query: 121 DANRCVKHLNQSILEGRYITVEKNKLCELCWQQSKMMSSSMSTLVKQLGQ 167
           DA RC+KHLNQS+LEGRYITVE+     +CWQQS+MMS SMSTLVKQL Q
Sbjct: 121 DAERCIKHLNQSVLEGRYITVER-----VCWQQSQMMSLSMSTLVKQLDQ 164

BLAST of CmaCh03G006500 vs. ExPASy TrEMBL
Match: A0A6J1BUT3 (serine/arginine-rich splicing factor SR45a isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005727 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 7.2e-60
Identity = 132/143 (92.31%), Postives = 137/143 (95.80%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAP--SRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGEN 60
           MADSHRKRYSRSPSPWRAP  SRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGR ++
Sbjct: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGR-DH 60

Query: 61  VNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDA 120
             N GNTLYVTGLSTRVTERDLEEHFSKEGKV+SCFLVVEPRTRISRGFAFVTMDNVDDA
Sbjct: 61  ALNTGNTLYVTGLSTRVTERDLEEHFSKEGKVASCFLVVEPRTRISRGFAFVTMDNVDDA 120

Query: 121 NRCVKHLNQSILEGRYITVEKNK 142
           NRCVKHLNQSILEGRYITVEK++
Sbjct: 121 NRCVKHLNQSILEGRYITVEKSR 142

BLAST of CmaCh03G006500 vs. NCBI nr
Match: XP_022950652.1 (serine/arginine-rich splicing factor SR45a [Cucurbita moschata] >XP_022978005.1 serine/arginine-rich splicing factor SR45a [Cucurbita maxima] >XP_023543883.1 serine/arginine-rich splicing factor SR45a [Cucurbita pepo subsp. pepo] >KAG6603882.1 Serine/arginine-rich splicing factor SR45a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.8 bits (673), Expect = 9.7e-67
Identity = 139/141 (98.58%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN 60
           MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN
Sbjct: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGENVN 60

Query: 61  NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR 120
           NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR
Sbjct: 61  NPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANR 120

Query: 121 CVKHLNQSILEGRYITVEKNK 142
           CVKHLNQSILEGRYITVEK++
Sbjct: 121 CVKHLNQSILEGRYITVEKSR 141

BLAST of CmaCh03G006500 vs. NCBI nr
Match: XP_038881911.1 (serine/arginine-rich splicing factor SR45a [Benincasa hispida])

HSP 1 Score: 241.9 bits (616), Expect = 3.9e-60
Identity = 132/143 (92.31%), Postives = 138/143 (96.50%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAP--SRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGEN 60
           MADSHRKRYSRSPSPWRAP  SRSRSRSRSRPRSRSRSWSRPRS SRGRSRSRSRGR ++
Sbjct: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRSRPRSRSRSWSRPRSPSRGRSRSRSRGR-DH 60

Query: 61  VNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDA 120
            +NPGNTLYVTGLSTRVTERDLEEHFSKEGKV+SCFLVVEPRTRISRGFAFVTMDNVDDA
Sbjct: 61  AHNPGNTLYVTGLSTRVTERDLEEHFSKEGKVASCFLVVEPRTRISRGFAFVTMDNVDDA 120

Query: 121 NRCVKHLNQSILEGRYITVEKNK 142
           NRCVKHLNQSILEGRYITVEK++
Sbjct: 121 NRCVKHLNQSILEGRYITVEKSR 142

BLAST of CmaCh03G006500 vs. NCBI nr
Match: XP_031743118.1 (serine/arginine-rich splicing factor SR45a [Cucumis sativus])

HSP 1 Score: 241.5 bits (615), Expect = 5.1e-60
Identity = 132/143 (92.31%), Postives = 137/143 (95.80%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAP--SRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGEN 60
           MAD HRKRYSRSPSPW+AP  SRSRSRSRSRPRSRSRSWSRPRS SRGRSRSRSRGR E+
Sbjct: 1   MADVHRKRYSRSPSPWKAPSRSRSRSRSRSRPRSRSRSWSRPRSPSRGRSRSRSRGR-EH 60

Query: 61  VNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDA 120
            NNPGNTLYVTGLSTRVTERDLEEHFSKEGKV+SCFLVVEPRTRISRGFAFVTMDNVDDA
Sbjct: 61  ANNPGNTLYVTGLSTRVTERDLEEHFSKEGKVASCFLVVEPRTRISRGFAFVTMDNVDDA 120

Query: 121 NRCVKHLNQSILEGRYITVEKNK 142
           NRCVKHLNQSILEGRYITVEK++
Sbjct: 121 NRCVKHLNQSILEGRYITVEKSR 142

BLAST of CmaCh03G006500 vs. NCBI nr
Match: KAE8647621.1 (hypothetical protein Csa_003197 [Cucumis sativus])

HSP 1 Score: 241.5 bits (615), Expect = 5.1e-60
Identity = 132/143 (92.31%), Postives = 137/143 (95.80%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAP--SRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRGEN 60
           MAD HRKRYSRSPSPW+AP  SRSRSRSRSRPRSRSRSWSRPRS SRGRSRSRSRGR E+
Sbjct: 1   MADVHRKRYSRSPSPWKAPSRSRSRSRSRSRPRSRSRSWSRPRSPSRGRSRSRSRGR-EH 60

Query: 61  VNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDA 120
            NNPGNTLYVTGLSTRVTERDLEEHFSKEGKV+SCFLVVEPRTRISRGFAFVTMDNVDDA
Sbjct: 61  ANNPGNTLYVTGLSTRVTERDLEEHFSKEGKVASCFLVVEPRTRISRGFAFVTMDNVDDA 120

Query: 121 NRCVKHLNQSILEGRYITVEKNK 142
           NRCVKHLNQSILEGRYITVEK++
Sbjct: 121 NRCVKHLNQSILEGRYITVEKSR 142

BLAST of CmaCh03G006500 vs. NCBI nr
Match: KAG7034061.1 (Ankyrin repeat domain-containing protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240.7 bits (613), Expect = 8.8e-60
Identity = 139/185 (75.14%), Postives = 139/185 (75.14%), Query Frame = 0

Query: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRG------ 60
           MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRG      
Sbjct: 1   MADSHRKRYSRSPSPWRAPSRSRSRSRSRPRSRSRSWSRPRSRSRGRSRSRSRGRCVCFF 60

Query: 61  ----------------------------------------RGENVNNPGNTLYVTGLSTR 120
                                                   RGENVNNPGNTLYVTGLSTR
Sbjct: 61  GWNSRQNNQRSISVELDLFVLLSHILLVFDSPWITFLASSRGENVNNPGNTLYVTGLSTR 120

Query: 121 VTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRY 140
           VTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRY
Sbjct: 121 VTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRY 180

BLAST of CmaCh03G006500 vs. TAIR 10
Match: AT4G35785.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 182.6 bits (462), Expect = 2.6e-46
Identity = 110/150 (73.33%), Postives = 126/150 (84.00%), Query Frame = 0

Query: 1   MADS-HRKRYSRSPSP----WRAPSRSRSRSRSRPRSRSRSWSRPR----SRSRGRSRSR 60
           MADS  R+R SRSPSP     R+ SRSRSRSRSRPR RSRS S PR    SRSRGRSRSR
Sbjct: 1   MADSPQRRRDSRSPSPRKERARSRSRSRSRSRSRPRLRSRSRSLPRPVSPSRSRGRSRSR 60

Query: 61  SRGRGENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVT 120
           SRGR E V NPG TLYVTGLSTRVT++DLE HF+KEGKV+SCFLV+EPRTR+SRGFAFVT
Sbjct: 61  SRGRSE-VENPGTTLYVTGLSTRVTDKDLEAHFAKEGKVASCFLVMEPRTRVSRGFAFVT 120

Query: 121 MDNVDDANRCVKHLNQSILEGRYITVEKNK 142
           M ++ DA RC+K+LNQS+LEGRYITVE+++
Sbjct: 121 MSSLKDAERCIKYLNQSVLEGRYITVERSR 149

BLAST of CmaCh03G006500 vs. TAIR 10
Match: AT4G35785.3 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 181.8 bits (460), Expect = 4.5e-46
Identity = 111/151 (73.51%), Postives = 125/151 (82.78%), Query Frame = 0

Query: 1   MADS-HRKRYSRSPSP----WRAPSRSRSRSRSRPRSRSRSWSRPR----SRSRGRSRSR 60
           MADS  R+R SRSPSP     R+ SRSRSRSRSRPR RSRS S PR    SRSRGRSRSR
Sbjct: 1   MADSPQRRRDSRSPSPRKERARSRSRSRSRSRSRPRLRSRSRSLPRPVSPSRSRGRSRSR 60

Query: 61  SRGRGENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVT 120
           SRGR E V NPG TLYVTGLSTRVT++DLE HF+KEGKV+SCFLV+EPRTR+SRGFAFVT
Sbjct: 61  SRGRSE-VENPGTTLYVTGLSTRVTDKDLEAHFAKEGKVASCFLVMEPRTRVSRGFAFVT 120

Query: 121 MDNVDDANRCVKHLNQSILEGRYITVEKNKL 143
           M ++ DA RC+K+LNQS+LEGRYITVE+  L
Sbjct: 121 MSSLKDAERCIKYLNQSVLEGRYITVERKIL 150

BLAST of CmaCh03G006500 vs. TAIR 10
Match: AT4G35785.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 179.5 bits (454), Expect = 2.2e-45
Identity = 106/148 (71.62%), Postives = 122/148 (82.43%), Query Frame = 0

Query: 1   MADS-HRKRYSRSPSP----WRAPSRSRSRSRSRP--RSRSRSWSRPRSRSRGRSRSRSR 60
           MADS  R+R SRSPSP     R+ SRSRSRSRSRP  RSRSRS  RP S SR R RSRSR
Sbjct: 1   MADSPQRRRDSRSPSPRKERARSRSRSRSRSRSRPRLRSRSRSLPRPVSPSRSRGRSRSR 60

Query: 61  GRGENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVEPRTRISRGFAFVTMD 120
            RG  V NPG TLYVTGLSTRVT++DLE HF+KEGKV+SCFLV+EPRTR+SRGFAFVTM 
Sbjct: 61  SRGSEVENPGTTLYVTGLSTRVTDKDLEAHFAKEGKVASCFLVMEPRTRVSRGFAFVTMS 120

Query: 121 NVDDANRCVKHLNQSILEGRYITVEKNK 142
           ++ DA RC+K+LNQS+LEGRYITVE+++
Sbjct: 121 SLKDAERCIKYLNQSVLEGRYITVERSR 148

BLAST of CmaCh03G006500 vs. TAIR 10
Match: AT4G35785.5 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.1e-43
Identity = 109/164 (66.46%), Postives = 123/164 (75.00%), Query Frame = 0

Query: 1   MADS-HRKRYSRSPSP----WRAPSRSRSRSRSRPRSRSRSWSRPR----SRSRGR---- 60
           MADS  R+R SRSPSP     R+ SRSRSRSRSRPR RSRS S PR    SRSRGR    
Sbjct: 1   MADSPQRRRDSRSPSPRKERARSRSRSRSRSRSRPRLRSRSRSLPRPVSPSRSRGRILRG 60

Query: 61  ---------SRSRSRGRGENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVE 120
                     RSRSR RG  V NPG TLYVTGLSTRVT++DLE HF+KEGKV+SCFLV+E
Sbjct: 61  NLFFITCTLCRSRSRSRGSEVENPGTTLYVTGLSTRVTDKDLEAHFAKEGKVASCFLVME 120

Query: 121 PRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRYITVEKNKL 143
           PRTR+SRGFAFVTM ++ DA RC+K+LNQS+LEGRYITVE+  L
Sbjct: 121 PRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVERKIL 164

BLAST of CmaCh03G006500 vs. TAIR 10
Match: AT4G35785.4 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 172.6 bits (436), Expect = 2.7e-43
Identity = 110/178 (61.80%), Postives = 128/178 (71.91%), Query Frame = 0

Query: 1   MADS-HRKRYSRSPSP----WRAPSRSRSRSRSRPRSRSRSWSRPR----SRSRGR---- 60
           MADS  R+R SRSPSP     R+ SRSRSRSRSRPR RSRS S PR    SRSRGR    
Sbjct: 1   MADSPQRRRDSRSPSPRKERARSRSRSRSRSRSRPRLRSRSRSLPRPVSPSRSRGRILRG 60

Query: 61  ---------SRSRSRGRGENVNNPGNTLYVTGLSTRVTERDLEEHFSKEGKVSSCFLVVE 120
                     RSRSR RG  V NPG TLYVTGLSTRVT++DLE HF+KEGKV+SCFLV+E
Sbjct: 61  NLFFITCTLCRSRSRSRGSEVENPGTTLYVTGLSTRVTDKDLEAHFAKEGKVASCFLVME 120

Query: 121 PRTRISRGFAFVTMDNVDDANRCVKHLNQSILEGRYITVEKNKLCELCWQQSKMMSSS 157
           PRTR+SRGFAFVTM ++ DA RC+K+LNQS+LEGRYITVE+  L +   +  K+   +
Sbjct: 121 PRTRVSRGFAFVTMSSLKDAERCIKYLNQSVLEGRYITVERKILHQATIRDDKIQEKA 178

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TH49.5e-2555.28Serine/arginine-rich splicing factor SR45a OS=Arabidopsis thaliana OX=3702 GN=SR... [more]
Q3ZBT61.1e-1245.03Transformer-2 protein homolog beta OS=Bos taurus OX=9913 GN=TRA2B PE=2 SV=1[more]
P629951.1e-1245.03Transformer-2 protein homolog beta OS=Homo sapiens OX=9606 GN=TRA2B PE=1 SV=1[more]
P629961.1e-1245.03Transformer-2 protein homolog beta OS=Mus musculus OX=10090 GN=Tra2b PE=1 SV=1[more]
P629971.1e-1245.03Transformer-2 protein homolog beta OS=Rattus norvegicus OX=10116 GN=Tra2b PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1INV24.7e-6798.58serine/arginine-rich splicing factor SR45a OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GFF04.7e-6798.58serine/arginine-rich splicing factor SR45a OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0KMM42.5e-6092.31RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507340 PE=4 SV... [more]
A0A251ND005.5e-6080.00RRM domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_7G175500 PE=4 S... [more]
A0A6J1BUT37.2e-6092.31serine/arginine-rich splicing factor SR45a isoform X1 OS=Momordica charantia OX=... [more]
Match NameE-valueIdentityDescription
XP_022950652.19.7e-6798.58serine/arginine-rich splicing factor SR45a [Cucurbita moschata] >XP_022978005.1 ... [more]
XP_038881911.13.9e-6092.31serine/arginine-rich splicing factor SR45a [Benincasa hispida][more]
XP_031743118.15.1e-6092.31serine/arginine-rich splicing factor SR45a [Cucumis sativus][more]
KAE8647621.15.1e-6092.31hypothetical protein Csa_003197 [Cucumis sativus][more]
KAG7034061.18.8e-6075.14Ankyrin repeat domain-containing protein, chloroplastic [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
AT4G35785.22.6e-4673.33RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G35785.34.5e-4673.51RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G35785.12.2e-4571.62RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G35785.52.1e-4366.46RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G35785.42.7e-4361.80RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 65..138
e-value: 3.8E-25
score: 99.5
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 66..135
e-value: 1.5E-18
score: 66.4
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 64..142
score: 16.636576
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 3..142
e-value: 1.9E-29
score: 104.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..50
NoneNo IPR availablePANTHERPTHR48034TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATEDcoord: 2..141
NoneNo IPR availablePANTHERPTHR48034:SF15OS06G0724600 PROTEINcoord: 2..141
NoneNo IPR availableCDDcd12382RRM_RBMX_likecoord: 63..141
e-value: 5.08943E-40
score: 128.11
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 10..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G006500.1CmaCh03G006500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding