CmaCh03G006280 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh03G006280
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionisocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like
LocationCma_Chr03: 5438109 .. 5443112 (+)
RNA-Seq ExpressionCmaCh03G006280
SyntenyCmaCh03G006280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTTCAAAATTGGGCGGCGGCGGCGGAGGCGGCGGGGACAGCGCAACCAAGAATATCGGAGGACAACAACCGATGGTTGTTGTTTTTCCAACGTTGGCCATTTCTTGTTCTTTTAATTGTCTGTTCATTTTAAGGCTTCATTCATCAGCAATGAGATGACAAACCAAACCATATAATTTCTTTGACAAAGAATAATATTGGCCTTCTGGCCTAAAATTCCACCTTCAATAATAATTTTATTTTTAATAATATAATTAATTAATGTTTTCACTAAATTTTTTGAATAAAAATAATATCAAATTAAATAACTTAAAACCATATCTACTGGCCACTGAATACTTGAGTGGTAACAAATGGTATTAGAACTAGACACGGGGTGGTGTGTCATAAAATGCTGGGCCTCCAAAGGCTGTTATCAATAGTATTAGAGCCAAATATCGAATAATGTGTTGAAGACAGTAGACCTCCAAGGGAATGGATTGTAGTAATATCTATTGCGTGGTGGGCTCGAGTTGTTACAATTGTAGCCTGCAGAAGAAGTACCTACACTTGCATAATATAGTGTTGATAACGGAATCAAAACTAGTGGTTTTTGAGCCTATCGTGTATCCTAATGCTACGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTAAATGACTCCTAAATTGTAGAGACATTATGCGGATAGCGCCGACATTAAGCAAGTCAACTATTGGTCAGTAGTACGAGTTTCACGAAAATCCAGTGATCTTGTCCGCTCTGGAACATACCTCACCCTTTCCTTCCTTTTCTATTGGCTGTCTAACGCTGATTTTTATTTTTCTCCCTTTGGACTTTCTCATCCCGCTGTTCTTCACCCTCTCCGGCGTCCCTCTAATGACCTATCGCGATTGTTAGACCACCGTCACGCCGCCGGAACGCTAAATAGCTCGACTCTGACAACTCTTTTCCAGCGATGGCTCGAAGATCCGTACCTATCCTCAAGCAGCTTCTCTCCAAATCCTCCGCTTCCGATAGTCCTGCCCTTTTGTCCTCATGTTTCAAATCCCCAATCGATGCTCATAATAGATCCGTCACTTATATGCCCCGACCTGGAGACGGCGCTCCCCGAACCGTTACCTTGATTCCCGGAGACGGAATTGGCCCCTTGGTTACCGGCGCCGTGGAGCAAGTCATGGATGCGATGCACGCCCCTTTGTATTTCGAGAAGTTTGAGGTTCATGGCGACATGAAGAAGGTTCCCCAAGAAGCAATTGACTCGATTAAGAAGAATAAAGTGTGTTTGAAGGGAGGATTGGTGACCCCTGTTGGCGGTGGTGTGAGTTCGCTGAATGTGCAGCTGAGGAAGGAGCTTGATCTCTATGCGTCGCTTGTGAATTGCTTTAATCTTCCTGGGCTTCCTACCAGGCATGAGAATGTGGATATTGTTGTGATTAGGGAGAATACAGAGGGTGAGTACGCAGGTTTGGAGCACGAGGTAGTTCCTGGCGTCGTGGAAAGCCTCAAGGTACTTTTATCTTTACGCGCAATGCTAGTTATTTCAATTTGATCCCCTAATTGAGTTACCATGTTCACAATTCCAAGCTTCTATAGTTTTTATTGAGCGTTATTGAGTGGAGTCCCACATTGGCTAATTAGGGGTCGGTCATGAATTTATAGGTAAAGAATATATCTCCAATAGGACGAGGCCTTCTGGAAAAACCAAAATCAAAGTCATGAGAGCTTATTCTCAAAGTAGATAATATCATACCATTGTGGAGATTTGTTATCCATAACGTGGTATCAGAGTCATGCCCTTAACTTAGCCATGTCAATAAAATTCTAAAATGTAGAATAAAAGGTGTACTTTGTTCGGAGTCGAACCTCGATTAAGGGGAGGCTGTTCGAGGACTCCATAGGCCTCATGGGAGGCTCTATAGTGTACTTTGTTCAAGGGGAGAATTGTTGAGGATTGTTGAGAGAGGGTCTCACATCGGTTAATTAAGGGGTTAATCATGGGTTTATAAGTAAGGAATACATCTCTATTGGCATGAGGCTTTTTGTGAAAACTAAAATCAAAGTGGATAGTATCATACCATTGTGAAGATTTGTGGTTCCTAACAGTTTTTCCTGTTTTCTGATGAATGTTCCCGTTTTTTCTAGCAATATATACACATTCGAGGTGCATGTTTGGACGAGGTTGTGCTCATTAGAACTTTATGATTTAGATGTTTTTCTACTTTCCATTATGAGGTTCTTTCCCCCTTTATCTTAGTATCTTTGTCTCTCATACTCACTCTATTGCCAGGTGATTACAAAGTTCTGTTCTGAGCGCATTGCTAAATATGCTTTTGAGTATGCTTACCTGAACAACAGAAAGAAAGTTACGGCCGTGCACAAAGCAAATATCATGAAGCTTGCAGATGGTCTGTTTCTAGAATCTTGTCGAGAGGTTGCCAGAAAGTATCCTGGAATCAAGTACAATGAAGTTATTGTCGACAATTGCTGCATGCAACTTGTCTCAAAGCCTGAGCAGTTTGATGTCATGGTATGCACTTGTACAATTGAATCACATTGTAAGGTCTTAATACATGGTCAAGAACATGCAGCGAATTTGTGTTCACATTTTGAAATGGTTGGGTGATTCCAATGGTCAAATATCATTTGATGATGTCTTACTGGGACGGGCAGGTGCTCCCTTTTTCCTTCTCCACTACTATTATAACAGCCCAAGGCTACCGCTAGCAAATATTGTCCGCTTTAGCTTGTTCCGTGTCCCTCTCCAATCAACGTGGGATCTCACAATCCACCCTCCTTGGGACCCAGCGTCCTTGCTGGCTCCGATACCATTTGTAACAGTCCAAGCCCACTTCTAGTAGATATTGTCTGCTTTAGCCCGTTATATATCGTCGTTAGCCTCACGATTTTTAAAACACGTCTATTAGGGAGAGGTTTCCACATCCTTATAAGATATGCTTCGTTCCCCTCTCCAACCGATGTGGGATCTCACACCTTCATTTCTTCCTTGTCTATTACATCCACTTATATGGGGAAGGAGGAAAAGAAAAAACCTAGATAATCTTATTTATGATTGTTCCCTTCTGGGAGAGGAAGGAGGTAAGAGGTAGAAAGCTTGGGGGTGGGAATTGAAATAAGTTCCCCTCTGTTCCGTGGATTACTAATTTCATTGATTGATGCATCCTTTCCTTTCATAGAAAAGTTACATTTCGATTATCACAGATTTGGAAAGTAGGAGATGATATTTGATTATGATTGGATATAATTTTGCAGGTGACACCCAATCTGTATGGAAATCTTGTAGCAAACACAGCTGCTGGTATAGCAGGTGGAACAGGAGTTATGCCGGGAGGTATGCTCAAATCTTTCACAGATTCAGATTCAATGCTTACATTTTAGCATTTCTCAAGTTTTTATTAGAAACCTTAATTAAGGCAGACACAGCATATGGCATATCATGTCACGTACTTGCCCTTTTCATCTTGTATGTCAACAAATTTAAGATGGATAGAAGGTGAAATTGTTTTATTTCCTTCATCCGCAACTGGGAATATTGCAGATGGGTATGCAATATTGAAAAATAACTGTTGAAATTCTGTCTCCCCGAGCTCGGAGTCGCTTTGTTTTGGTTTCTTATGAGGTAGGAGATTAAATATTGCTGGATTCTTGTTACAACTCAAGCCTACCGCTAGCAGATATTGTTCTCTGAGCTTTCACTTATGGGTTTCCCCTAAAGGTTTTAAAACGCGTCTGCTAGGGAGAGGTTTTCATACCCTTATAAATAATGTTTCGATCTCCTCTCCAACCGACGTGGGATCTCACAATTTGGAGTGTTCTTTATTCAGGCAATGTTGGTGCTGATCACGCTGTTTTTGAACAAGGAGCTTCAGCAGGAAATGTGGGGAATGAGAGACTAGTGGAACAAAAGAAGGCAAACCCTGTAGCGCTGCTCTTGTCATCTGCCATGATGCTGAGACATCTCCAGTTTCCTTCTTTTGCGGATCGATTAGAGACAGCGGTAGAGAAGGTGATATCCGAAGGCAAGTATCGGACCAAGGACCTCGGCGGGCAGAGCACGACTCAAGAGGTTGTTGATGCAGTCATAGCTGCTTTAGATTGATCAAGTAAGGGAGAATAACTTACCCCACCATTCATAAACAATTTATACCATTTTTGGGTGGTCAAAATCCTGCCCAGTTTCCTGAATCAAGAAACAAGATATGCCTTCGCCTTTGAATTATTATTATGATTTGTTTCAGTTTTCCCAAGCACACTAGAGTTGGGAATGCATTTCTTGTTCCAATAATCTCGATTCAAGACAACAATTTGTGGTGTATTTGATTTGCAAAATTTGAAGAGAGAGCCATTCTATTTTACTTGGATTTGGGTGTGTTTCTTATTTATAAATTCATTATCATTC

mRNA sequence

ATGAATGTTCAAAATTGGGCGGCGGCGGCGGAGGCGGCGGGGACAGCGCAACCAAGAATATCGGAGGACAACAACCGATGGTTGTTGTTTTTCCAACGTTGGCCATTTCTTGTTCTTTTAATTCTCGACTCTGACAACTCTTTTCCAGCGATGGCTCGAAGATCCGTACCTATCCTCAAGCAGCTTCTCTCCAAATCCTCCGCTTCCGATAGTCCTGCCCTTTTGTCCTCATGTTTCAAATCCCCAATCGATGCTCATAATAGATCCGTCACTTATATGCCCCGACCTGGAGACGGCGCTCCCCGAACCGTTACCTTGATTCCCGGAGACGGAATTGGCCCCTTGGTTACCGGCGCCGTGGAGCAAGTCATGGATGCGATGCACGCCCCTTTGTATTTCGAGAAGTTTGAGGTTCATGGCGACATGAAGAAGGTTCCCCAAGAAGCAATTGACTCGATTAAGAAGAATAAAGTGTGTTTGAAGGGAGGATTGGTGACCCCTGTTGGCGGTGGTGTGAGTTCGCTGAATGTGCAGCTGAGGAAGGAGCTTGATCTCTATGCGTCGCTTGTGAATTGCTTTAATCTTCCTGGGCTTCCTACCAGGCATGAGAATGTGGATATTGTTGTGATTAGGGAGAATACAGAGGGTGAGTACGCAGGTTTGGAGCACGAGGTAGTTCCTGGCGTCGTGGAAAGCCTCAAGGTGATTACAAAGTTCTGTTCTGAGCGCATTGCTAAATATGCTTTTGAGTATGCTTACCTGAACAACAGAAAGAAAGTTACGGCCGTGCACAAAGCAAATATCATGAAGCTTGCAGATGGTCTGTTTCTAGAATCTTGTCGAGAGGTTGCCAGAAAGTATCCTGGAATCAAGTACAATGAAGTTATTGTCGACAATTGCTGCATGCAACTTGTCTCAAAGCCTGAGCAGTTTGATGTCATGGTGACACCCAATCTGTATGGAAATCTTGTAGCAAACACAGCTGCTGGTATAGCAGGTGGAACAGGAGTTATGCCGGGAGGCAATGTTGGTGCTGATCACGCTGTTTTTGAACAAGGAGCTTCAGCAGGAAATGTGGGGAATGAGAGACTAGTGGAACAAAAGAAGGCAAACCCTGTAGCGCTGCTCTTGTCATCTGCCATGATGCTGAGACATCTCCAGTTTCCTTCTTTTGCGGATCGATTAGAGACAGCGGTAGAGAAGGTGATATCCGAAGGCAAGTATCGGACCAAGGACCTCGGCGGGCAGAGCACGACTCAAGAGGTTGTTGATGCAGTCATAGCTGCTTTAGATTGATCAAGTAAGGGAGAATAACTTACCCCACCATTCATAAACAATTTATACCATTTTTGGGTGGTCAAAATCCTGCCCAGTTTCCTGAATCAAGAAACAAGATATGCCTTCGCCTTTGAATTATTATTATGATTTGTTTCAGTTTTCCCAAGCACACTAGAGTTGGGAATGCATTTCTTGTTCCAATAATCTCGATTCAAGACAACAATTTGTGGTGTATTTGATTTGCAAAATTTGAAGAGAGAGCCATTCTATTTTACTTGGATTTGGGTGTGTTTCTTATTTATAAATTCATTATCATTC

Coding sequence (CDS)

ATGAATGTTCAAAATTGGGCGGCGGCGGCGGAGGCGGCGGGGACAGCGCAACCAAGAATATCGGAGGACAACAACCGATGGTTGTTGTTTTTCCAACGTTGGCCATTTCTTGTTCTTTTAATTCTCGACTCTGACAACTCTTTTCCAGCGATGGCTCGAAGATCCGTACCTATCCTCAAGCAGCTTCTCTCCAAATCCTCCGCTTCCGATAGTCCTGCCCTTTTGTCCTCATGTTTCAAATCCCCAATCGATGCTCATAATAGATCCGTCACTTATATGCCCCGACCTGGAGACGGCGCTCCCCGAACCGTTACCTTGATTCCCGGAGACGGAATTGGCCCCTTGGTTACCGGCGCCGTGGAGCAAGTCATGGATGCGATGCACGCCCCTTTGTATTTCGAGAAGTTTGAGGTTCATGGCGACATGAAGAAGGTTCCCCAAGAAGCAATTGACTCGATTAAGAAGAATAAAGTGTGTTTGAAGGGAGGATTGGTGACCCCTGTTGGCGGTGGTGTGAGTTCGCTGAATGTGCAGCTGAGGAAGGAGCTTGATCTCTATGCGTCGCTTGTGAATTGCTTTAATCTTCCTGGGCTTCCTACCAGGCATGAGAATGTGGATATTGTTGTGATTAGGGAGAATACAGAGGGTGAGTACGCAGGTTTGGAGCACGAGGTAGTTCCTGGCGTCGTGGAAAGCCTCAAGGTGATTACAAAGTTCTGTTCTGAGCGCATTGCTAAATATGCTTTTGAGTATGCTTACCTGAACAACAGAAAGAAAGTTACGGCCGTGCACAAAGCAAATATCATGAAGCTTGCAGATGGTCTGTTTCTAGAATCTTGTCGAGAGGTTGCCAGAAAGTATCCTGGAATCAAGTACAATGAAGTTATTGTCGACAATTGCTGCATGCAACTTGTCTCAAAGCCTGAGCAGTTTGATGTCATGGTGACACCCAATCTGTATGGAAATCTTGTAGCAAACACAGCTGCTGGTATAGCAGGTGGAACAGGAGTTATGCCGGGAGGCAATGTTGGTGCTGATCACGCTGTTTTTGAACAAGGAGCTTCAGCAGGAAATGTGGGGAATGAGAGACTAGTGGAACAAAAGAAGGCAAACCCTGTAGCGCTGCTCTTGTCATCTGCCATGATGCTGAGACATCTCCAGTTTCCTTCTTTTGCGGATCGATTAGAGACAGCGGTAGAGAAGGTGATATCCGAAGGCAAGTATCGGACCAAGGACCTCGGCGGGCAGAGCACGACTCAAGAGGTTGTTGATGCAGTCATAGCTGCTTTAGATTGA

Protein sequence

MNVQNWAAAAEAAGTAQPRISEDNNRWLLFFQRWPFLVLLILDSDNSFPAMARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGDGIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRTKDLGGQSTTQEVVDAVIAALD
Homology
BLAST of CmaCh03G006280 vs. ExPASy Swiss-Prot
Match: Q8LFC0 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH1 PE=1 SV=2)

HSP 1 Score: 629.8 bits (1623), Expect = 2.3e-179
Identity = 318/381 (83.46%), Postives = 343/381 (90.03%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           M+RRS+ +LK L   ++ S                  RSVTYMPRPGDGAPR VTLIPGD
Sbjct: 1   MSRRSLTLLKNLARNANGS--------------GIQTRSVTYMPRPGDGAPRAVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVT AVEQVM+AMHAP++FEK++VHG+M +VP E ++SI+KNKVCLKGGL TPVGG
Sbjct: 61  GIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEMSRVPPEVMESIRKNKVCLKGGLKTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDL+ASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA+KYP I
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPSI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV+KPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 TYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVG +++V + KANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI+EGK RT
Sbjct: 301 EQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIAEGKCRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGG STTQEVVDAVIA LD
Sbjct: 361 KDLGGTSTTQEVVDAVIAKLD 367

BLAST of CmaCh03G006280 vs. ExPASy Swiss-Prot
Match: O81796 (Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH3 PE=1 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.5e-178
Identity = 312/381 (81.89%), Postives = 348/381 (91.34%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSV I  +LL+                SP  + +RS+TYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVSIFNRLLANP-------------PSPFTSLSRSITYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVM+AMHAP++FE++EV G+M+KVP+E I+S+K+NKVCLKGGL TPVGG
Sbjct: 61  GIGPLVTGAVEQVMEAMHAPVHFERYEVLGNMRKVPEEVIESVKRNKVCLKGGLATPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLN+QLRKELD++ASLVNC N+PGL TRHENVDIVVIRENTEGEY+GLEHEVVPGVV
Sbjct: 121 GVSSLNMQLRKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA+ Y GI
Sbjct: 181 ESLKVITKFCSERIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV+KPEQFDVMVTPNLYGNL+ANTAAGIAGGTGVMPGGNVGA+HA+F
Sbjct: 241 TYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGN+++VEQKKANPVALLLSSAMMLRHL+FP+FADRLETAV++VI EGKYRT
Sbjct: 301 EQGASAGNVGNDKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGG  TTQEVVDAVIAAL+
Sbjct: 361 KDLGGDCTTQEVVDAVIAALE 368

BLAST of CmaCh03G006280 vs. ExPASy Swiss-Prot
Match: P93032 (Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH2 PE=1 SV=2)

HSP 1 Score: 609.4 bits (1570), Expect = 3.2e-173
Identity = 310/381 (81.36%), Postives = 336/381 (88.19%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           M+R+S  +LK L S +S S                  RSVTYMPRPGDG PR VTLIPGD
Sbjct: 1   MSRQSFSLLKNLRSIASGS--------------KIQTRSVTYMPRPGDGKPRPVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           G+GPLVT AV+QVM+AMHAP+YFE FEVHGDMK +P+  ++SIKKNKVCLKGGL TPVGG
Sbjct: 61  GVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNV LRKELDL+ASLVNCFNLPGL +RHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA+KYP I
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGA++AVF
Sbjct: 241 AYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVG +   EQK ANPVALLLSSAMMLRHLQFPSFADRLETAV++VI+EG  RT
Sbjct: 301 EQGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           +DLGG STTQEVVDAVIA LD
Sbjct: 361 EDLGGNSTTQEVVDAVIANLD 367

BLAST of CmaCh03G006280 vs. ExPASy Swiss-Prot
Match: Q54B68 (Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=idhB PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.3e-91
Identity = 178/328 (54.27%), Postives = 230/328 (70.12%), Query Frame = 0

Query: 102 RTVTLIPGDGIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLK 161
           +TVT+IPGDGIGP +T +V  V  A   P+ +E F++ G  + + QE I SI +NKV LK
Sbjct: 30  KTVTVIPGDGIGPEITSSVMGVFQAAKVPIEWEIFDISGG-QPISQELIASITRNKVALK 89

Query: 162 GGLVTPVGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHEN--VDIVVIRENTEGEYA 221
           G L T +  G  S N++LRK LDLYA +V C  +PG+  RH++  VD VVIRENT+GEY+
Sbjct: 90  GPLYTEILSGSQSRNMELRKALDLYAHVVPCKQIPGITARHDDVLVDFVVIRENTQGEYS 149

Query: 222 GLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLES 281
           GLE  + PGVV+SLK+ITK  SERIA+YAFEYA  N RKKVTAVHKANI K  DGLFL +
Sbjct: 150 GLEQVLTPGVVQSLKIITKEASERIARYAFEYAKANGRKKVTAVHKANIQKQTDGLFLAT 209

Query: 282 CREVARKYPGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMP 341
           C ++A++YP IK+   I+DNCCMQLV  PEQ+DVMVTPNLYGN+V+N  A + GG G+  
Sbjct: 210 CTQIAKEYPEIKFENTIIDNCCMQLVKSPEQYDVMVTPNLYGNIVSNIGAALVGGPGLAG 269

Query: 342 GGNVGADHAVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV 401
           G NVG    +FE GA   +V  + +  + KANP  LLL+S MML+HL     A ++E AV
Sbjct: 270 GANVGEGSIIFEMGAH--HVAAD-IAGKDKANPTGLLLASVMMLKHLGLNEHATKVENAV 329

Query: 402 EKVISEGKYRTKDLGGQSTTQEVVDAVI 428
           + VI EG   T D+GG+S+T++   AVI
Sbjct: 330 KAVIKEGTL-TSDIGGKSSTKQFTGAVI 352

BLAST of CmaCh03G006280 vs. ExPASy Swiss-Prot
Match: Q945K7 (Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH5 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.7e-89
Identity = 168/333 (50.45%), Postives = 232/333 (69.67%), Query Frame = 0

Query: 101 PRTVTLIPGDGIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQ-----EAIDSIKK 160
           P T TL PGDGIGP +  +V++V      P+ +E+  V  ++    Q     E+++S+++
Sbjct: 43  PITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRR 102

Query: 161 NKVCLKGGLVTPVGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTE 220
           NKV LKG + TP+G G  SLN+ LRKEL+LYA++  C++LPG  TR+++VD++ IRENTE
Sbjct: 103 NKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTE 162

Query: 221 GEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGL 280
           GEY+GLEH+VV GVVESLK+IT+  S R+A+YAF YA  + R++V+A+HKANIM+  DGL
Sbjct: 163 GEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGL 222

Query: 281 FLESCREVARKYPGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGT 340
           FL+ CREVA KYP I Y EV++DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG 
Sbjct: 223 FLKCCREVAEKYPEITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGL 282

Query: 341 GVMPGGNVGADHAVFEQG--ASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFAD 400
           G+ P  N+G D     +    SA ++  + L     ANP ALLLS  MMLRHL+F   A+
Sbjct: 283 GLTPSCNIGEDGVALAEAVHGSAPDIAGKNL-----ANPTALLLSGVMMLRHLKFNEQAE 342

Query: 401 RLETAVEKVISEGKYRTKDLGGQSTTQEVVDAV 427
           ++ +A+   I+EGKYRT DLGG STT E   A+
Sbjct: 343 QIHSAIINTIAEGKYRTADLGGSSTTTEFTKAI 370

BLAST of CmaCh03G006280 vs. ExPASy TrEMBL
Match: A0A6J1IJI3 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111477663 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.6e-213
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEVVDAVIAALD
Sbjct: 361 KDLGGQSTTQEVVDAVIAALD 381

BLAST of CmaCh03G006280 vs. ExPASy TrEMBL
Match: A0A6J1GDS7 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111453080 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.1e-212
Identity = 379/381 (99.48%), Postives = 380/381 (99.74%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSVPILKQLLSKSSASDSPALL SCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVPILKQLLSKSSASDSPALLFSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVISEGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVISEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEVVDAVIAALD
Sbjct: 361 KDLGGQSTTQEVVDAVIAALD 381

BLAST of CmaCh03G006280 vs. ExPASy TrEMBL
Match: A0A5A7SJ01 (Isocitrate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00670 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.0e-206
Identity = 368/384 (95.83%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 48  FPAMARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLI 107
           FPAMARRS+PILKQLLSKS +S++PALLSSCFKS IDA NRSVTYMPRPGDGAPR VTLI
Sbjct: 37  FPAMARRSLPILKQLLSKSPSSENPALLSSCFKSSIDAQNRSVTYMPRPGDGAPRAVTLI 96

Query: 108 PGDGIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTP 167
           PGDGIGPLVTGAVEQVMDAMHAP+YFEKF+VHGDMKKVPQE IDSIKKNKVCLKGGLVTP
Sbjct: 97  PGDGIGPLVTGAVEQVMDAMHAPVYFEKFDVHGDMKKVPQEVIDSIKKNKVCLKGGLVTP 156

Query: 168 VGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVP 227
           VGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEY+GLEHEVVP
Sbjct: 157 VGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVP 216

Query: 228 GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKY 287
           GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA KY
Sbjct: 217 GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATKY 276

Query: 288 PGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADH 347
           PGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADH
Sbjct: 277 PGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADH 336

Query: 348 AVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGK 407
           AVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI EGK
Sbjct: 337 AVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIFEGK 396

Query: 408 YRTKDLGGQSTTQEVVDAVIAALD 432
           YRTKDLGGQSTTQEVVDAVIA+LD
Sbjct: 397 YRTKDLGGQSTTQEVVDAVIASLD 420

BLAST of CmaCh03G006280 vs. ExPASy TrEMBL
Match: A0A0A0KMQ6 (Iso_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G509550 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 7.3e-205
Identity = 364/381 (95.54%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRS+PILKQLLSKSS+S++PALLSSCFKS IDA +RSVTYMPRPGDGAPR VTLIPGD
Sbjct: 1   MARRSLPILKQLLSKSSSSENPALLSSCFKSSIDAQSRSVTYMPRPGDGAPRAVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAP+YFEKFE+HGDMKKVPQE IDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPVYFEKFEIHGDMKKVPQEVIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEY+GLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA KYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI EGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIFEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEV+DAVIA+LD
Sbjct: 361 KDLGGQSTTQEVIDAVIASLD 381

BLAST of CmaCh03G006280 vs. ExPASy TrEMBL
Match: A0A1S3B2U5 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103485186 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 9.6e-205
Identity = 365/381 (95.80%), Postives = 374/381 (98.16%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRS+PILKQLLSKS +S++PALLSSCFKS IDA NRSVTYMPRPGDGAPR VTLIPGD
Sbjct: 1   MARRSLPILKQLLSKSPSSENPALLSSCFKSSIDAQNRSVTYMPRPGDGAPRAVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAP+YFEKF+VHGDMKKVPQE IDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPVYFEKFDVHGDMKKVPQEVIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEY+GLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA KYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI EGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIFEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEVVDAVIA+LD
Sbjct: 361 KDLGGQSTTQEVVDAVIASLD 381

BLAST of CmaCh03G006280 vs. NCBI nr
Match: XP_022977301.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita maxima] >XP_023544985.1 isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023544986.1 isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 750.0 bits (1935), Expect = 1.2e-212
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEVVDAVIAALD
Sbjct: 361 KDLGGQSTTQEVVDAVIAALD 381

BLAST of CmaCh03G006280 vs. NCBI nr
Match: KAG6603865.1 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034045.1 Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746.5 bits (1926), Expect = 1.3e-211
Identity = 379/381 (99.48%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAH+RSVTYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHSRSVTYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVISEGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVISEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEVVDAVIAALD
Sbjct: 361 KDLGGQSTTQEVVDAVIAALD 381

BLAST of CmaCh03G006280 vs. NCBI nr
Match: XP_022949790.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 746.1 bits (1925), Expect = 1.7e-211
Identity = 379/381 (99.48%), Postives = 380/381 (99.74%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSVPILKQLLSKSSASDSPALL SCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVPILKQLLSKSSASDSPALLFSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVISEGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVISEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEVVDAVIAALD
Sbjct: 361 KDLGGQSTTQEVVDAVIAALD 381

BLAST of CmaCh03G006280 vs. NCBI nr
Match: KAA0025614.1 (isocitrate dehydrogenase [Cucumis melo var. makuwa] >TYK12489.1 isocitrate dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 729.2 bits (1881), Expect = 2.1e-206
Identity = 368/384 (95.83%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 48  FPAMARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLI 107
           FPAMARRS+PILKQLLSKS +S++PALLSSCFKS IDA NRSVTYMPRPGDGAPR VTLI
Sbjct: 37  FPAMARRSLPILKQLLSKSPSSENPALLSSCFKSSIDAQNRSVTYMPRPGDGAPRAVTLI 96

Query: 108 PGDGIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTP 167
           PGDGIGPLVTGAVEQVMDAMHAP+YFEKF+VHGDMKKVPQE IDSIKKNKVCLKGGLVTP
Sbjct: 97  PGDGIGPLVTGAVEQVMDAMHAPVYFEKFDVHGDMKKVPQEVIDSIKKNKVCLKGGLVTP 156

Query: 168 VGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVP 227
           VGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEY+GLEHEVVP
Sbjct: 157 VGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVP 216

Query: 228 GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKY 287
           GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA KY
Sbjct: 217 GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATKY 276

Query: 288 PGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADH 347
           PGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADH
Sbjct: 277 PGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADH 336

Query: 348 AVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGK 407
           AVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI EGK
Sbjct: 337 AVFEQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIFEGK 396

Query: 408 YRTKDLGGQSTTQEVVDAVIAALD 432
           YRTKDLGGQSTTQEVVDAVIA+LD
Sbjct: 397 YRTKDLGGQSTTQEVVDAVIASLD 420

BLAST of CmaCh03G006280 vs. NCBI nr
Match: XP_004134829.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial [Cucumis sativus] >KGN48986.1 hypothetical protein Csa_003560 [Cucumis sativus])

HSP 1 Score: 723.0 bits (1865), Expect = 1.5e-204
Identity = 364/381 (95.54%), Postives = 375/381 (98.43%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRS+PILKQLLSKSS+S++PALLSSCFKS IDA +RSVTYMPRPGDGAPR VTLIPGD
Sbjct: 1   MARRSLPILKQLLSKSSSSENPALLSSCFKSSIDAQSRSVTYMPRPGDGAPRAVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVMDAMHAP+YFEKFE+HGDMKKVPQE IDSIKKNKVCLKGGLVTPVGG
Sbjct: 61  GIGPLVTGAVEQVMDAMHAPVYFEKFEIHGDMKKVPQEVIDSIKKNKVCLKGGLVTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEY+GLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA KYPGI
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATKYPGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
           KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI EGKYRT
Sbjct: 301 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIFEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGGQSTTQEV+DAVIA+LD
Sbjct: 361 KDLGGQSTTQEVIDAVIASLD 381

BLAST of CmaCh03G006280 vs. TAIR 10
Match: AT4G35260.1 (isocitrate dehydrogenase 1 )

HSP 1 Score: 629.8 bits (1623), Expect = 1.6e-180
Identity = 318/381 (83.46%), Postives = 343/381 (90.03%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           M+RRS+ +LK L   ++ S                  RSVTYMPRPGDGAPR VTLIPGD
Sbjct: 1   MSRRSLTLLKNLARNANGS--------------GIQTRSVTYMPRPGDGAPRAVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVT AVEQVM+AMHAP++FEK++VHG+M +VP E ++SI+KNKVCLKGGL TPVGG
Sbjct: 61  GIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEMSRVPPEVMESIRKNKVCLKGGLKTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNVQLRKELDL+ASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA+KYP I
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPSI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV+KPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF
Sbjct: 241 TYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVG +++V + KANPVALLLSSAMMLRHLQFPSFADRLETAV+KVI+EGK RT
Sbjct: 301 EQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIAEGKCRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGG STTQEVVDAVIA LD
Sbjct: 361 KDLGGTSTTQEVVDAVIAKLD 367

BLAST of CmaCh03G006280 vs. TAIR 10
Match: AT4G35650.1 (isocitrate dehydrogenase III )

HSP 1 Score: 627.1 bits (1616), Expect = 1.1e-179
Identity = 312/381 (81.89%), Postives = 348/381 (91.34%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           MARRSV I  +LL+                SP  + +RS+TYMPRPGDGAPRTVTLIPGD
Sbjct: 1   MARRSVSIFNRLLANP-------------PSPFTSLSRSITYMPRPGDGAPRTVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           GIGPLVTGAVEQVM+AMHAP++FE++EV G+M+KVP+E I+S+K+NKVCLKGGL TPVGG
Sbjct: 61  GIGPLVTGAVEQVMEAMHAPVHFERYEVLGNMRKVPEEVIESVKRNKVCLKGGLATPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLN+QLRKELD++ASLVNC N+PGL TRHENVDIVVIRENTEGEY+GLEHEVVPGVV
Sbjct: 121 GVSSLNMQLRKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA+ Y GI
Sbjct: 181 ESLKVITKFCSERIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV+KPEQFDVMVTPNLYGNL+ANTAAGIAGGTGVMPGGNVGA+HA+F
Sbjct: 241 TYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVGN+++VEQKKANPVALLLSSAMMLRHL+FP+FADRLETAV++VI EGKYRT
Sbjct: 301 EQGASAGNVGNDKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           KDLGG  TTQEVVDAVIAAL+
Sbjct: 361 KDLGGDCTTQEVVDAVIAALE 368

BLAST of CmaCh03G006280 vs. TAIR 10
Match: AT2G17130.1 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 609.4 bits (1570), Expect = 2.3e-174
Identity = 310/381 (81.36%), Postives = 336/381 (88.19%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           M+R+S  +LK L S +S S                  RSVTYMPRPGDG PR VTLIPGD
Sbjct: 1   MSRQSFSLLKNLRSIASGS--------------KIQTRSVTYMPRPGDGKPRPVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           G+GPLVT AV+QVM+AMHAP+YFE FEVHGDMK +P+  ++SIKKNKVCLKGGL TPVGG
Sbjct: 61  GVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNV LRKELDL+ASLVNCFNLPGL +RHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA+KYP I
Sbjct: 181 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGA++AVF
Sbjct: 241 AYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVG +   EQK ANPVALLLSSAMMLRHLQFPSFADRLETAV++VI+EG  RT
Sbjct: 301 EQGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           +DLGG STTQEVVDAVIA LD
Sbjct: 361 EDLGGNSTTQEVVDAVIANLD 367

BLAST of CmaCh03G006280 vs. TAIR 10
Match: AT2G17130.2 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 596.7 bits (1537), Expect = 1.5e-170
Identity = 306/381 (80.31%), Postives = 332/381 (87.14%), Query Frame = 0

Query: 51  MARRSVPILKQLLSKSSASDSPALLSSCFKSPIDAHNRSVTYMPRPGDGAPRTVTLIPGD 110
           M+R+S  +LK L S +S S                  RSVTYMPRPGDG PR VTLIPGD
Sbjct: 1   MSRQSFSLLKNLRSIASGS--------------KIQTRSVTYMPRPGDGKPRPVTLIPGD 60

Query: 111 GIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQEAIDSIKKNKVCLKGGLVTPVGG 170
           G+GPLVT AV+QVM+AMHAP+YFE FEVHGDMK +P+  ++SIKKNKVCLKGGL TPVGG
Sbjct: 61  GVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGG 120

Query: 171 GVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVV 230
           GVSSLNV LRKELDL+ASLVNCFNLPGL +RHENVDIVVIRENTEGEYAGLEHEVVPGVV
Sbjct: 121 GVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVV 180

Query: 231 ESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVARKYPGI 290
           ESL    KFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA+KYP I
Sbjct: 181 ESL----KFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSI 240

Query: 291 KYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVF 350
            YNE+IVDNCCMQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGA++AVF
Sbjct: 241 AYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVF 300

Query: 351 EQGASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVEKVISEGKYRT 410
           EQGASAGNVG +   EQK ANPVALLLSSAMMLRHLQFPSFADRLETAV++VI+EG  RT
Sbjct: 301 EQGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRT 360

Query: 411 KDLGGQSTTQEVVDAVIAALD 432
           +DLGG STTQEVVDAVIA LD
Sbjct: 361 EDLGGNSTTQEVVDAVIANLD 363

BLAST of CmaCh03G006280 vs. TAIR 10
Match: AT5G03290.1 (isocitrate dehydrogenase V )

HSP 1 Score: 331.3 bits (848), Expect = 1.2e-90
Identity = 168/333 (50.45%), Postives = 232/333 (69.67%), Query Frame = 0

Query: 101 PRTVTLIPGDGIGPLVTGAVEQVMDAMHAPLYFEKFEVHGDMKKVPQ-----EAIDSIKK 160
           P T TL PGDGIGP +  +V++V      P+ +E+  V  ++    Q     E+++S+++
Sbjct: 43  PITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRR 102

Query: 161 NKVCLKGGLVTPVGGGVSSLNVQLRKELDLYASLVNCFNLPGLPTRHENVDIVVIRENTE 220
           NKV LKG + TP+G G  SLN+ LRKEL+LYA++  C++LPG  TR+++VD++ IRENTE
Sbjct: 103 NKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTE 162

Query: 221 GEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGL 280
           GEY+GLEH+VV GVVESLK+IT+  S R+A+YAF YA  + R++V+A+HKANIM+  DGL
Sbjct: 163 GEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGL 222

Query: 281 FLESCREVARKYPGIKYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGT 340
           FL+ CREVA KYP I Y EV++DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG 
Sbjct: 223 FLKCCREVAEKYPEITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGL 282

Query: 341 GVMPGGNVGADHAVFEQG--ASAGNVGNERLVEQKKANPVALLLSSAMMLRHLQFPSFAD 400
           G+ P  N+G D     +    SA ++  + L     ANP ALLLS  MMLRHL+F   A+
Sbjct: 283 GLTPSCNIGEDGVALAEAVHGSAPDIAGKNL-----ANPTALLLSGVMMLRHLKFNEQAE 342

Query: 401 RLETAVEKVISEGKYRTKDLGGQSTTQEVVDAV 427
           ++ +A+   I+EGKYRT DLGG STT E   A+
Sbjct: 343 QIHSAIINTIAEGKYRTADLGGSSTTTEFTKAI 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LFC02.3e-17983.46Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Arabidopsi... [more]
O817961.5e-17881.89Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial OS=Arabidopsi... [more]
P930323.2e-17381.36Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial OS=Arabidopsi... [more]
Q54B682.3e-9154.27Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial OS=Dictyostel... [more]
Q945K71.7e-8950.45Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis... [more]
Match NameE-valueIdentityDescription
A0A6J1IJI35.6e-213100.00isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1GDS78.1e-21299.48isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A5A7SJ011.0e-20695.83Isocitrate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0KMQ67.3e-20595.54Iso_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G509550 PE=3... [more]
A0A1S3B2U59.6e-20595.80isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Cucumis me... [more]
Match NameE-valueIdentityDescription
XP_022977301.11.2e-212100.00isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
KAG6603865.11.3e-21199.48Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cuc... [more]
XP_022949790.11.7e-21199.48isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
KAA0025614.12.1e-20695.83isocitrate dehydrogenase [Cucumis melo var. makuwa] >TYK12489.1 isocitrate dehyd... [more]
XP_004134829.11.5e-20495.54isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial [Cucumis sati... [more]
Match NameE-valueIdentityDescription
AT4G35260.11.6e-18083.46isocitrate dehydrogenase 1 [more]
AT4G35650.11.1e-17981.89isocitrate dehydrogenase III [more]
AT2G17130.12.3e-17481.36isocitrate dehydrogenase subunit 2 [more]
AT2G17130.21.5e-17080.31isocitrate dehydrogenase subunit 2 [more]
AT5G03290.11.2e-9050.45isocitrate dehydrogenase V [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024084Isopropylmalate dehydrogenase-like domainSMARTSM01329Iso_dh_2coord: 103..426
e-value: 2.7E-147
score: 505.3
IPR024084Isopropylmalate dehydrogenase-like domainPFAMPF00180Iso_dhcoord: 103..426
e-value: 1.9E-81
score: 273.9
IPR004434Isocitrate dehydrogenase NAD-dependentTIGRFAMTIGR00175TIGR00175coord: 100..429
e-value: 6.9E-157
score: 519.8
NoneNo IPR availableGENE3D3.40.718.10Isopropylmalate Dehydrogenasecoord: 90..430
e-value: 1.6E-129
score: 433.7
NoneNo IPR availablePANTHERPTHR11835DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATEcoord: 84..429
NoneNo IPR availablePANTHERPTHR11835:SF56NAD-DEPENDENT ISOCITRATE DEHYDROGENASE C,1coord: 84..429
NoneNo IPR availableSUPERFAMILY53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 102..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G006280.1CmaCh03G006280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006102 isocitrate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004449 isocitrate dehydrogenase (NAD+) activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor