CmaCh03G002570 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G002570 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 3.6e-121
Identity = 233/435 (53.56%), Postives = 314/435 (72.18%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDRESIE+WLFS +  +CPVT+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHT+RR+IQ+WCT+NA  GIERIPTPKPPI   ++ +++ ++  S  +Q  CL+RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNV---------EFSLED--YAYLIDDQF-- 183
            I + + +NKRC+EAA   EFLA IV NS +            +L D   + +++++F  
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 -ANLGDEALSILYKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRAYSVMLLTSM 243
             +L DEALS+LY L  SE+ LK LL N  G   V TLTKI+Q G Y SRAY+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G I 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLL-DLDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC+CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

BLAST of CmaCh03G002570 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.4e-117
Identity = 219/421 (52.02%), Postives = 306/421 (72.68%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR+SIE+WLF+ +  +CPVT+  ++  D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHT+RR+IQ+WCT+NA  G+ERIPTP+PPI   ++ +++ D+  S  +Q  CL+RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSILYK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L DEAL++LY 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG I V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC+CA+GRAE LNH A IAVV KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQ V  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

BLAST of CmaCh03G002570 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.8e-59
Identity = 161/456 (35.31%), Postives = 245/456 (53.73%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I +WL  ++  +CPVT+  L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 61  -SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAK---LSPFSQQ 120
              D+TPNH +RR+IQ WC  N  +G+ RI TP+ P     VV  + + K        ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 121 NCLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDE 180
             L++L  + A   +N+R M   G  + L   V   T+ +   ED    I        DE
Sbjct: 121 ETLQKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSED---EDGRRRIKGL-----DE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           +L +L+ + I  +  K +L  N  + + +LT +L    + S+AY+++LL ++ E      
Sbjct: 181 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 240

Query: 241 ILSLNTDFFIEIVQILKDQIS-----------------------------------KQAS 300
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 241 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 300

Query: 301 KSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSSEKRSCEMILVTLDLLCRCADG 360
            +AL +L+   S SRNR   V  GA+  LI+L +   + EKR  E++L  L  LC CA+G
Sbjct: 301 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 360

Query: 361 RAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCF 418
           RAE+L H  GIAVV+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC 
Sbjct: 361 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 420

BLAST of CmaCh03G002570 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 9.8e-50
Identity = 150/424 (35.38%), Postives = 226/424 (53.30%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCPVT+  LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAK------LSPFSQQN 123
           + PNHT+RR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEA 183
            LRRLR  A  S+ N+  + A  A E L  I+ + T                 + L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR---AYSVMLLTSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R   A  + ++++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLCR  +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

BLAST of CmaCh03G002570 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.0e-46
Identity = 146/423 (34.52%), Postives = 224/423 (52.96%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  + TCPVT+V LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAK------LSPFSQQN 123
           + PNHT+RR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEA 183
            +RRLR +A  SE N+  +    A E L  I+    ++E +            + L  E+
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL--FADIETT---------SLSSELVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ- 243
           L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  +  
Sbjct: 188 LALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL 247

Query: 244 --ILSLNTDFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLID- 303
             I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID 
Sbjct: 248 KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLCR  +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSC 416
            L  +A  +A E    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S 
Sbjct: 368 AL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDST 410

BLAST of CmaCh03G002570 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.4e-232
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180
           RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300
           NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of CmaCh03G002570 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.4e-226
Identity = 406/423 (95.98%), Postives = 417/423 (98.58%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120
           +FDITPNHT+RRVIQAWCT+NACKG+ERIPTPKPP+DCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180
           RL+SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of CmaCh03G002570 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.7e-193
Identity = 354/416 (85.10%), Postives = 380/416 (91.35%), Query Frame = 0

Query: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFDITP 66
           E+DPPPFFLCPISLQIMKDPVTVASGITYDRE+IERWLFSD+H TCPVTQVVLS FDITP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 67  NHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLRSIA 126
           N+T+ R+IQAWCT+N    +ERIPTPKPP+D KQVV+ILAD KLSPFSQQNCLRRL SIA
Sbjct: 63  NNTLSRLIQAWCTVN--NRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 122

Query: 127 AASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSILYKLQI 186
           +A+E NKRCMEAAGAVEFL G+VCNS  +E S  D+A+  DD  ANLGDEALSILYKLQI
Sbjct: 123 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAF--DDPLANLGDEALSILYKLQI 182

Query: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFI 246
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSRAY+ MLL SMFEVAD+IQI SLN +FF+
Sbjct: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242

Query: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSSEKRSCE 306
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AVQAGA+PVLIDLLLDLDSSEKR CE
Sbjct: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCE 302

Query: 307 MILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESV 366
           MILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 303 MILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362

Query: 367 VQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 423
           VQEM RLGVV KLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 363 VQEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

BLAST of CmaCh03G002570 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.3e-190
Identity = 352/427 (82.44%), Postives = 390/427 (91.33%), Query Frame = 0

Query: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQV 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIE+WLFSD+HKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQN 120
           VLS FD+TPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDE 180
           CL RLRSIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN  DE
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLD 300
           I +L  DFFIEI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LC CADGRAE+LNHAAGIAVVSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 423
           +LRS++P
Sbjct: 421 TLRSAYP 425

BLAST of CmaCh03G002570 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.3e-190
Identity = 352/427 (82.44%), Postives = 390/427 (91.33%), Query Frame = 0

Query: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQV 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIE+WLFSD+HKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQN 120
           VLS FD+TPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDE 180
           CL RLRSIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN  DE
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLD 300
           I +L  DFFIEI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LC CADGRAE+LNHAAGIAVVSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 423
           +LRS++P
Sbjct: 421 TLRSAYP 425

BLAST of CmaCh03G002570 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 815.1 bits (2104), Expect = 2.9e-232
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180
           RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300
           NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of CmaCh03G002570 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.7 bits (2072), Expect = 1.5e-228
Identity = 412/423 (97.40%), Postives = 420/423 (99.29%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSDRHKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPNHT+RRVIQAWCT+NACKGIERIPTPKPP+DCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180
           RLRSIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQFANLGDEALSI
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PV+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of CmaCh03G002570 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 792.7 bits (2046), Expect = 1.5e-225
Identity = 406/423 (95.98%), Postives = 417/423 (98.58%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120
           +FDITPNHT+RRVIQAWCT+NACKG+ERIPTPKPP+DCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180
           RL+SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of CmaCh03G002570 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 792.7 bits (2046), Expect = 1.5e-225
Identity = 408/423 (96.45%), Postives = 418/423 (98.82%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD HKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60

Query: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPN T+RRVIQAWCT+NACKGIERIPTPKPP+DCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180
           RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           L+KLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of CmaCh03G002570 vs. NCBI nr
Match: KAG6603504.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 746.9 bits (1927), Expect = 9.6e-212
Identity = 386/401 (96.26%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 23  MKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFDITPNHTIRRVIQAWCTINA 82
           MKDPVTVASGITYDRESIE+WLFSD HKTCPVTQVVLSSFDITPN T+RRVIQAWCT+NA
Sbjct: 1   MKDPVTVASGITYDRESIEKWLFSDGHKTCPVTQVVLSSFDITPNITLRRVIQAWCTVNA 60

Query: 83  CKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLRSIAAASESNKRCMEAAGAV 142
           CKGIERIPTPKPP+DCKQVVRILADAKLSPFSQQNCLRRLRSIAAASESNKRCMEAAGAV
Sbjct: 61  CKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAAASESNKRCMEAAGAV 120

Query: 143 EFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSILYKLQISESGLKFLLANNGGIF 202
           EFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLGDEALSILYKLQISESGLKFLLANNGGIF
Sbjct: 121 EFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILYKLQISESGLKFLLANNGGIF 180

Query: 203 VSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFIEIVQILKDQISKQASK 262
           V+TLTKILQNGTYGSRAYSVMLLTSMFEVADQIQI+SLNTD FIEIVQILKDQISKQASK
Sbjct: 181 VTTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIVSLNTDIFIEIVQILKDQISKQASK 240

Query: 263 SALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSSEKRSCEMILVTLDLLCRCADGR 322
           SALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSSEKRSCEMILVTLDLLC CADGR
Sbjct: 241 SALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGR 300

Query: 323 AEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL 382
           AEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL
Sbjct: 301 AEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL 360

Query: 383 LQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH 424
           LQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH
Sbjct: 361 LQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH 401

BLAST of CmaCh03G002570 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 436.4 bits (1121), Expect = 2.6e-122
Identity = 233/435 (53.56%), Postives = 314/435 (72.18%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDRESIE+WLFS +  +CPVT+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHT+RR+IQ+WCT+NA  GIERIPTPKPPI   ++ +++ ++  S  +Q  CL+RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNV---------EFSLED--YAYLIDDQF-- 183
            I + + +NKRC+EAA   EFLA IV NS +            +L D   + +++++F  
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 -ANLGDEALSILYKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRAYSVMLLTSM 243
             +L DEALS+LY L  SE+ LK LL N  G   V TLTKI+Q G Y SRAY+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G I 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLL-DLDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC+CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

BLAST of CmaCh03G002570 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 424.5 bits (1090), Expect = 1.0e-118
Identity = 219/421 (52.02%), Postives = 306/421 (72.68%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR+SIE+WLF+ +  +CPVT+  ++  D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHT+RR+IQ+WCT+NA  G+ERIPTP+PPI   ++ +++ D+  S  +Q  CL+RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSILYK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L DEAL++LY 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG I V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC+CA+GRAE LNH A IAVV KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQ V  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

BLAST of CmaCh03G002570 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-60
Identity = 161/456 (35.31%), Postives = 245/456 (53.73%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I +WL  ++  +CPVT+  L 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 74

Query: 61  -SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAK---LSPFSQQ 120
              D+TPNH +RR+IQ WC  N  +G+ RI TP+ P     VV  + + K        ++
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 134

Query: 121 NCLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDE 180
             L++L  + A   +N+R M   G  + L   V   T+ +   ED    I        DE
Sbjct: 135 ETLQKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSED---EDGRRRIKGL-----DE 194

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           +L +L+ + I  +  K +L  N  + + +LT +L    + S+AY+++LL ++ E      
Sbjct: 195 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 254

Query: 241 ILSLNTDFFIEIVQILKDQIS-----------------------------------KQAS 300
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 255 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 314

Query: 301 KSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSSEKRSCEMILVTLDLLCRCADG 360
            +AL +L+   S SRNR   V  GA+  LI+L +   + EKR  E++L  L  LC CA+G
Sbjct: 315 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 374

Query: 361 RAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCF 418
           RAE+L H  GIAVV+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC 
Sbjct: 375 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 434

BLAST of CmaCh03G002570 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 199.1 bits (505), Expect = 6.9e-51
Identity = 150/424 (35.38%), Postives = 226/424 (53.30%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCPVT+  LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAK------LSPFSQQN 123
           + PNHT+RR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEA 183
            LRRLR  A  S+ N+  + A  A E L  I+ + T                 + L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR---AYSVMLLTSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R   A  + ++++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLCR  +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

BLAST of CmaCh03G002570 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 189.1 bits (479), Expect = 7.2e-48
Identity = 146/423 (34.52%), Postives = 224/423 (52.96%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  + TCPVT+V LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAK------LSPFSQQN 123
           + PNHT+RR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEA 183
            +RRLR +A  SE N+  +    A E L  I+    ++E +            + L  E+
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL--FADIETT---------SLSSELVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ- 243
           L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  +  
Sbjct: 188 LALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL 247

Query: 244 --ILSLNTDFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLID- 303
             I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID 
Sbjct: 248 KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLCR  +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSC 416
            L  +A  +A E    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S 
Sbjct: 368 AL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDST 410