CmaCh03G001230 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G001230
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	EF hand calcium-binding family protein
Location	Cma_Chr03: 1285869 .. 1286447 (-)
RNA-Seq Expression	CmaCh03G001230
Synteny	CmaCh03G001230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCCCTCCGCCGACGTCTCCCGCTCCGATCCGCCGTCCTTCGGCTCTGGTACGTCTTCCTCCTCCTCTTCGTCCGAGAATTTCTTCAAGCCTCACACCGCTGGCTACGGAACTCCGACCAGCGTCCTCGCTGGCTGTTCCGACTCAGACCAGCGGGAGAACGACGTCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTTTGAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGACGGCGGATTTATCCGGTTGGATGAATTGATGGCTCGGGTCGGGTCGGGGTTCGATGCGGTAGGGGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTTGACGCGGATCATGATGGGAGGATCTCGGCGGAGGAGCTTCACGGGGTTTATAGATCAATTGGGGATGAGAGGTGCACGTTAGAGGACTGTCGGCGTATGATAAGGGATGTGGATGAGGACGGGGATGGGTTTGTGTGCTTCACGGAGTTCGTGCGTATGATGGAGCTGCAGAGATGA

mRNA sequence

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCCCTCCGCCGACGTCTCCCGCTCCGATCCGCCGTCCTTCGGCTCTGGTACGTCTTCCTCCTCCTCTTCGTCCGAGAATTTCTTCAAGCCTCACACCGCTGGCTACGGAACTCCGACCAGCGTCCTCGCTGGCTGTTCCGACTCAGACCAGCGGGAGAACGACGTCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTTTGAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGACGGCGGATTTATCCGGTTGGATGAATTGATGGCTCGGGTCGGGTCGGGGTTCGATGCGGTAGGGGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTTGACGCGGATCATGATGGGAGGATCTCGGCGGAGGAGCTTCACGGGGTTTATAGATCAATTGGGGATGAGAGGTGCACGTTAGAGGACTGTCGGCGTATGATAAGGGATGTGGATGAGGACGGGGATGGGTTTGTGTGCTTCACGGAGTTCGTGCGTATGATGGAGCTGCAGAGATGA

Coding sequence (CDS)

ATGAAAATCCCCAAGCGATTTAGCCCTAAACGCCTCTTCCGATCCAAGAAGAATCCCTCCGCCGACGTCTCCCGCTCCGATCCGCCGTCCTTCGGCTCTGGTACGTCTTCCTCCTCCTCTTCGTCCGAGAATTTCTTCAAGCCTCACACCGCTGGCTACGGAACTCCGACCAGCGTCCTCGCTGGCTGTTCCGACTCAGACCAGCGGGAGAACGACGTCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTTTGAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGACGGCGGATTTATCCGGTTGGATGAATTGATGGCTCGGGTCGGGTCGGGGTTCGATGCGGTAGGGGAGTCGGAGCTCCGGGAGACGTTTGAGTTTTTTGACGCGGATCATGATGGGAGGATCTCGGCGGAGGAGCTTCACGGGGTTTATAGATCAATTGGGGATGAGAGGTGCACGTTAGAGGACTGTCGGCGTATGATAAGGGATGTGGATGAGGACGGGGATGGGTTTGTGTGCTTCACGGAGTTCGTGCGTATGATGGAGCTGCAGAGATGA

Protein sequence

MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVCFTEFVRMMELQR

Homology

[Close]

BLAST of CmaCh03G001230 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.1e-38
Identity = 107/210 (50.95%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MK+ K   PKR F   K+ S         SFGS +SSS    +N       G  TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  AGCSDS------------DQRENDVVVSRKELEAILSRIA----SEEEVAMMLSEVD-DG 120
                               R+ND  VSR +LE++LSR+     +EEE+ +ML EVD DG
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 -GFIRLDELMARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLE 180
            G IRL+EL +RV S   A   +EL+ETFEFFDAD DG ISA+EL  V+ +IGDERCTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

Query: 181 DCRRMIRDVDEDGDGFVCFTEFVRMMELQR 193
           DC+RMI DVDEDGDGFVCFTEF RMM+LQR
Sbjct: 181 DCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of CmaCh03G001230 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-36
Identity = 107/204 (52.45%), Postives = 130/204 (63.73%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSS--SSENFFKPHTAGYGTPTSVLAGC 65
           R SPKRLFR+K    A VSRS+P SF S  SSSSS  S  N  +  TA   TP SVL   
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLPQN 68

Query: 66  SDS-----------DQRENDVVVSRKELEAILSRIA----SEEEVAMMLSEVD--DGGFI 125
           S               R++D VVSR +L A++SR++    S+EEV++ML EVD  DGG I
Sbjct: 69  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 128

Query: 126 RLDELMARVG--SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDC 185
            L++L +RV   SG  +V   ELRE FE FD D +G+ISAEELH V+  IGDERCTLE+C
Sbjct: 129 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEEC 188

Query: 186 RRMIRDVDEDGDGFVCFTEFVRMM 189
            RMI  VD +GDGFVCF +F RMM
Sbjct: 189 MRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of CmaCh03G001230 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.4e-18
Identity = 56/128 (43.75%), Postives = 79/128 (61.72%), Query Frame = 0


Query: 69  RENDVVVSRKELEAI---LSRIASEEEVAMMLSE--VDDGGFIRLDELMARVGSGFDAVG 128
           + ND  +S  EL+ +   LS  AS+EE   M+ E  +D  GFI LDE +A       +  
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 129 ES---ELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC 188
            S   +L+E F+ +D D +GRISA ELH V +++G E+C+++DC+RMI  VD DGDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

[Close]

BLAST of CmaCh03G001230 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 3.4e-18
Identity = 54/118 (45.76%), Postives = 74/118 (62.71%), Query Frame = 0


Query: 78  KELEAILSRIASEEEVAMMLS--EVDDGGFIRLDELMARVGSGFDAVGE-----SELRET 137
           KE+   LS  AS EE   M+   ++D  GFI LDE +A    G    G      S+L+E 
Sbjct: 39  KEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEA 98

Query: 138 FEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVCFTEFVRMM 189
           FE +D D +GRISA+ELH V +++G E+C+++DC++MI  VD DGDG V F EF +MM
Sbjct: 99  FELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of CmaCh03G001230 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.5e-17
Identity = 58/135 (42.96%), Postives = 76/135 (56.30%), Query Frame = 0


Query: 72  DVVVSRKELEAILSRIAS---EEEVAMMLSEVDD--GGFIRLDELM--------ARVGSG 131
           D  +S +EL ++L  + +     EV  M+ E+D    GF+ L E          A  G G
Sbjct: 18  DGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAAFHCGPTPAHGGKG 77

Query: 132 FD-----AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDE 189
            D     A  E+ELRE F  +DAD +G+ISA ELH V R +GD +C++ DC RMIR VD 
Sbjct: 78  GDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGD-KCSVADCSRMIRSVDA 137

[Close]

BLAST of CmaCh03G001230 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.3e-100
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFD 120
           AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFD
Sbjct: 61  AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFD 120

Query: 121 AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC 180
           AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC
Sbjct: 121 AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC 180

Query: 181 FTEFVRMMELQR 193
           FTEFVRMMELQR
Sbjct: 181 FTEFVRMMELQR 192

[Close]

BLAST of CmaCh03G001230 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.9e-96
Identity = 189/193 (97.93%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV- 60
           MKIPKRFSPKRLFRSKKNPSA+VSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV 
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGF 120
           LAGCSDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD GGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV 180
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV 180

Query: 181 CFTEFVRMMELQR 193
           CFTEFVRMMELQR
Sbjct: 181 CFTEFVRMMELQR 193

[Close]

BLAST of CmaCh03G001230 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.8e-79
Identity = 168/196 (85.71%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDPPSFGSGTSSSSSSSENFFKP++   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG--GFIRLDELMARVG 120
           VL    DSD REN+ VV+RKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMARVG
Sbjct: 62  VL----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARVG 121

Query: 121 SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGD 180
           SG +A GESELRETFEFFDAD DGRI+AEELHGV+RSIGDE+CTLEDCRRMIRDVDE+GD
Sbjct: 122 SG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENGD 181

Query: 181 GFVCFTEFVRMMELQR 193
           GFVCF EFVRMMELQR
Sbjct: 182 GFVCFAEFVRMMELQR 192

[Close]

BLAST of CmaCh03G001230 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.8e-79
Identity = 168/196 (85.71%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDPPSFGSGTSSSSSSSENFFKP++   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG--GFIRLDELMARVG 120
           VL    DSD REN+ VV+RKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMARVG
Sbjct: 62  VL----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARVG 121

Query: 121 SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGD 180
           SG +A GESELRETFEFFDAD DGRI+AEELHGV+RSIGDE+CTLEDCRRMIRDVDE+GD
Sbjct: 122 SG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENGD 181

Query: 181 GFVCFTEFVRMMELQR 193
           GFVCF EFVRMMELQR
Sbjct: 182 GFVCFAEFVRMMELQR 192

[Close]

BLAST of CmaCh03G001230 vs. ExPASy TrEMBL
Match: A0A0A0L0H1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.4e-77
Identity = 166/196 (84.69%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDPPSFGSGTSSSSSSSENFFK +T   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61

Query: 61  VLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG--GFIRLDELMARVG 120
           VL    DSD R N+ VVSRKELEAILSRIASEEEVAMML EVD G  GFIRL+ELMARVG
Sbjct: 62  VL----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVG 121

Query: 121 SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGD 180
           SG +A GESE+RETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDC RMIRDVDE+GD
Sbjct: 122 SG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGD 181

Query: 181 GFVCFTEFVRMMELQR 193
           GFVCF EFVRMMELQR
Sbjct: 182 GFVCFAEFVRMMELQR 192

[Close]

BLAST of CmaCh03G001230 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 373.2 bits (957), Expect = 1.3e-99
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFD 120
           AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFD
Sbjct: 61  AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGFD 120

Query: 121 AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC 180
           AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC
Sbjct: 121 AVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC 180

Query: 181 FTEFVRMMELQR 193
           FTEFVRMMELQR
Sbjct: 181 FTEFVRMMELQR 192

[Close]

BLAST of CmaCh03G001230 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 361.7 bits (927), Expect = 3.9e-96
Identity = 189/193 (97.93%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV- 60
           MKIPKRFSPKRLFRSKKNPSA+VSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV 
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGF 120
           LAGCSDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD GGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV 180
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV 180

Query: 181 CFTEFVRMMELQR 193
           CFTEFVRMMELQR
Sbjct: 181 CFTEFVRMMELQR 193

[Close]

BLAST of CmaCh03G001230 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.8 bits (922), Expect = 1.5e-95
Identity = 188/193 (97.41%), Postives = 189/193 (97.93%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV- 60
           MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV 
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGF 120
           LAGCSDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD GGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV 180
           D+V ESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV
Sbjct: 121 DSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFV 180

Query: 181 CFTEFVRMMELQR 193
           CFTEFVRMMELQR
Sbjct: 181 CFTEFVRMMELQR 193

[Close]

BLAST of CmaCh03G001230 vs. NCBI nr
Match: KAG7033603.1 (putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323.2 bits (827), Expect = 1.6e-84
Identity = 171/175 (97.71%), Postives = 172/175 (98.29%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV- 60
           MKIPKRFSPKRLFRSKKNPSA+VSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSV 
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60

Query: 61  LAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGGFIRLDELMARVGSGF 120
           LAGCSDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD GGFIRLDELMARVGSGF
Sbjct: 61  LAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGGFIRLDELMARVGSGF 120

Query: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 175
           DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED
Sbjct: 121 DAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDED 175

[Close]

BLAST of CmaCh03G001230 vs. NCBI nr
Match: XP_008457589.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 304.7 bits (779), Expect = 5.7e-79
Identity = 168/196 (85.71%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHT--AGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDPPSFGSGTSSSSSSSENFFKP++   GYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG--GFIRLDELMARVG 120
           VL    DSD REN+ VV+RKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMARVG
Sbjct: 62  VL----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARVG 121

Query: 121 SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGD 180
           SG +A GESELRETFEFFDAD DGRI+AEELHGV+RSIGDE+CTLEDCRRMIRDVDE+GD
Sbjct: 122 SG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENGD 181

Query: 181 GFVCFTEFVRMMELQR 193
           GFVCF EFVRMMELQR
Sbjct: 182 GFVCFAEFVRMMELQR 192

[Close]

BLAST of CmaCh03G001230 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 159.1 bits (401), Expect = 3.6e-39
Identity = 107/210 (50.95%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTAGYGTPTSVL 60
           MK+ K   PKR F   K+ S         SFGS +SSS    +N       G  TPTS+L
Sbjct: 1   MKLAK-LIPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  AGCSDS------------DQRENDVVVSRKELEAILSRIA----SEEEVAMMLSEVD-DG 120
                               R+ND  VSR +LE++LSR+     +EEE+ +ML EVD DG
Sbjct: 61  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDG 120

Query: 121 -GFIRLDELMARVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLE 180
            G IRL+EL +RV S   A   +EL+ETFEFFDAD DG ISA+EL  V+ +IGDERCTL+
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

Query: 181 DCRRMIRDVDEDGDGFVCFTEFVRMMELQR 193
           DC+RMI DVDEDGDGFVCFTEF RMM+LQR
Sbjct: 181 DCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of CmaCh03G001230 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 154.1 bits (388), Expect = 1.2e-37
Identity = 107/204 (52.45%), Postives = 130/204 (63.73%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSS--SSENFFKPHTAGYGTPTSVLAGC 65
           R SPKRLFR+K    A VSRS+P SF S  SSSSS  S  N  +  TA   TP SVL   
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA---TPISVLPQN 68

Query: 66  SDS-----------DQRENDVVVSRKELEAILSRIA----SEEEVAMMLSEVD--DGGFI 125
           S               R++D VVSR +L A++SR++    S+EEV++ML EVD  DGG I
Sbjct: 69  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 128

Query: 126 RLDELMARVG--SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDC 185
            L++L +RV   SG  +V   ELRE FE FD D +G+ISAEELH V+  IGDERCTLE+C
Sbjct: 129 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEEC 188

Query: 186 RRMIRDVDEDGDGFVCFTEFVRMM 189
            RMI  VD +GDGFVCF +F RMM
Sbjct: 189 MRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of CmaCh03G001230 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-19
Identity = 56/128 (43.75%), Postives = 79/128 (61.72%), Query Frame = 0


Query: 69  RENDVVVSRKELEAI---LSRIASEEEVAMMLSE--VDDGGFIRLDELMARVGSGFDAVG 128
           + ND  +S  EL+ +   LS  AS+EE   M+ E  +D  GFI LDE +A       +  
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 129 ES---ELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVC 188
            S   +L+E F+ +D D +GRISA ELH V +++G E+C+++DC+RMI  VD DGDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

[Close]

BLAST of CmaCh03G001230 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-19
Identity = 54/118 (45.76%), Postives = 74/118 (62.71%), Query Frame = 0


Query: 78  KELEAILSRIASEEEVAMMLS--EVDDGGFIRLDELMARVGSGFDAVGE-----SELRET 137
           KE+   LS  AS EE   M+   ++D  GFI LDE +A    G    G      S+L+E 
Sbjct: 39  KEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEA 98

Query: 138 FEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVCFTEFVRMM 189
           FE +D D +GRISA+ELH V +++G E+C+++DC++MI  VD DGDG V F EF +MM
Sbjct: 99  FELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of CmaCh03G001230 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-16
Identity = 52/125 (41.60%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 72  DVVVSRKELEAILSRI---ASEEEVAMMLSEVD--DGGFIRLDELMARVGSGFDAVGESE 131
           D  +S KEL AI++ +     EEE+   ++E+D    G+I  +E +     G D     E
Sbjct: 50  DGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLE 109

Query: 132 -LRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGDGFVCFTEFV 191
            L++ F  +D D +G ISAEELH V RS+GDE C++ +CR+MI  VD+DGDG + F EF 
Sbjct: 110 NLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEFK 169

The following BLAST results are available for this feature:

BLAST of CmaCh03G001230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	5.1e-38	50.95	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	1.6e-36	52.45	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9C8Y1	3.4e-18	43.75	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	3.4e-18	45.76	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q0DJV6	6.5e-17	42.96	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CmaCh03G001230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HS37	6.3e-100	100.00	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A6J1F310	1.9e-96	97.93	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A5A7VA05	2.8e-79	85.71	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	2.8e-79	85.71	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]
A0A0A0L0H1	4.4e-77	84.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1	[more]

BLAST of CmaCh03G001230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022967907.1	1.3e-99	100.00	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_022932888.1	3.9e-96	97.93	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_023543601.1	1.5e-95	97.41	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
KAG7033603.1	1.6e-84	97.71	putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_008457589.1	5.7e-79	85.71	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 p...	[more]

BLAST of CmaCh03G001230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	3.6e-39	50.95	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	1.2e-37	52.45	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	2.4e-19	43.75	calmodulin like 23	[more]
AT5G37770.1	2.4e-19	45.76	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.1e-16	41.60	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 126..154 e-value: 4.5E-5 score: 32.9 coord: 163..191 e-value: 3.6E-5 score: 33.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 125..188 e-value: 2.4E-10 score: 40.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 159..192 score: 13.230217
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 122..157 score: 14.42972
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 126..188 e-value: 3.45553E-17 score: 70.2693
None	No IPR available	GENE3D	1.10.238.10		coord: 68..192 e-value: 1.9E-22 score: 81.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..50
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..190
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..190
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 135..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 172..184
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 86..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G001230.1	CmaCh03G001230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding