CmaCh02G017300 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G017300
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	BRCA1-associated RING domain protein 1-like
Location	Cma_Chr02: 9723674 .. 9725884 (-)
RNA-Seq Expression	CmaCh02G017300
Synteny	CmaCh02G017300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAATAATAGCAGGTTTTTTGAACCTGTGTTGCTTCCTTGCACTCACCTGTTTTGCAAGTATGCTTCCCATTTCGTTGTTTTTCATTCATCTTTCTTATTTTTGCGATTTGCAAGTTCTAAATACGTTCTTGACTTTGTTGTAGGGGTTGCATGCCATTTGCTTCCCAGATTGGATCAGTGTGCCCTCTCTGAAAATCAGGATTTGTCGATCGAAGTAGATTTTCCACTAGTCTTAGGCTTCTTGTAATCTTTCGTAAGATTTCTGGTATTTGTTGTTTTTTCTTTTTTGAGCAGCGAGGTCTTGAATTATCCCTGATTCTTCATCCTTTATGATTACCCTGTTTCATCTCGACTTTTCTTCTGAATATCTGATTTATTTACAAACAGGGTCATATTCAATACTTGAACATCGCCTTTCTTGATTGATTAGATTACGGGTGATTGTTCGATTTTGTATATGTATATATATATCTCGAGGGATGTTCGTGATGCATTTGTCGTATGCTATATTATGCAGACATGAGGCCTGCTCCTTTCATGGACAAGATGGTGTCAATATGCAGGAGCCTTGATGCCACATTCTCTACATATATGTCTAAACTAGTCTCACCTGTCAACAATTCTAAAGAAAAGGAATGCTGGGAACAATTCTAAAGAAAAGGAAGATAACAAGTGCGAAAAATATCATAATTGTCACATAGTACATTAATATCACAATTTACATGTTTTAAAATTTGCTTCCTATTTGTAGTATGCTGGGAACAATTTACTTACCGAGAATAAAGAAAGACCAGTTGCAATTTTAAGTAAATTTTCAACACGTAGTCTTAGTTCTTACCTCATTTATGTCTTCTGCATCTCATGGTCTGAAGATCATCCATCTTTTAAACTGCCCTAAGTGTCTAATTAACTCCAGTAGACGCTGGGGCAGCTGTGGACGAATCATGTTTTGGTCAGTCTATGCGTTACTCTTCCAGCAACTGCAAGGGGTTTGAGGGGTGCAGCATGCCTCTTGATTGTGAATGTGAGAATCTTGACATTGAGGGTAAAAAGGGTAGTGGGAGGCATGAAGATTGTAGAATGCCACCTGTGAGTCAGGCAAACCAGTTATCAGCCGGAAGCCCACCACACTTTGGTTCTGGCAAGGTATCAGATGACAGTAGTGAAGAGTACCGTGGTCGTGCAAGATTTGATATTCTCCCTATTTCCTCCTCCTCCCCTGTCTTTTTTCTGGTCATTTTCCCAGCTTTTGTTGCAAATGTAGGAAGTGCATGCTGGCTTCTAGCATCATTATAAGCTTCTAAACTATCTGTAGCTCTGAAGTGCCATCTTATTGTCGTATAGTGATAATTGCCTCTATATCATGTAGGTGCACAGAATTCTGTGTTGTACATATATTTCTGAATTTAGCAATTGGTAGTCTAGCCGAGAGGTGGTGTGATTGCCATACAATATCTTAGCGAAGGGTCTTTTCATCCATTTTCTGATAACTTGAAATTTGGAGTGTTTAGGGGTAAAAGCTTGCATGAAAGAAAGGCGTCCTTTGAACGAGGAAGCTTATGAAATTTCTCTTGACCACTATGGGTGCATTGATGGTCCCAAATCTGGCAGGCTGAGGGTTTTAAGCAATGTAAGTTGACTCATTGGATGCATGAAGAGTAATGGTATTAAAATCCTTACTGTCATTGTTACATTAGTAGAGTACAATTTTGTTGGTGGAGCGTGAGAGCATATATTTAAGGTTTCATTATGATTTCATTTATTGGTTAGGGTACTGTGGTCAATTGCATCTTCTGTTCCGTACTCGTCTGTGGTTCAATTGGTGATTTATGACCCGATTATTTCCCATTCCATTTCTACAGGCACCCAAGCTCTTCATTGGCTTGAGTTTCTATTTTACTGGTGATTTTCCATCGGCTTATGAGGAGGATCTTCAAGATTTGGTCAAAGCTGCGGGAGGTACCATATTAGAGAAGGACGAATTCCCAGCACCTAGTTGTAATGACCAGACTGCACCAAAGGTGCTGGTTGTATACAATCTCGATTCTCCTGGAGGATGCAAAGTGGGGGTGGAGGTTTTAATTCTCTGGCAAAGATTAAATGAGGCAGAGGGAATAGCTGCAAAGGTTGGAGCCCAAGTCATTGGTCATACATGGCTTGTGGAATCCATTGCAGCGGGTAACTTGCAACATTTTGTCAGCTGCTAA

mRNA sequence

ATGCTAATAATAGCAGGTTTTTTGAACCTGTGTTGCTTCCTTGCACTCACCTGTTTTGCAAGGGTTGCATGCCATTTGCTTCCCAGATTGGATCAGTGTGCCCTCTCTGAAAATCAGGATTTGTCGATCGAATATGCTGGGAACAATTTACTTACCGAGAATAAAGAAAGACCAGTTGCAATTTTAATAGACGCTGGGGCAGCTGTGGACGAATCATGTTTTGGTCAGTCTATGCGTTACTCTTCCAGCAACTGCAAGGGGTTTGAGGGGTGCAGCATGCCTCTTGATTGTGAATGTGAGAATCTTGACATTGAGGGTAAAAAGGGTAGTGGGAGGCATGAAGATTGTAGAATGCCACCTGTGAGTCAGGCAAACCAGTTATCAGCCGGAAGCCCACCACACTTTGGTTCTGGCAAGGTATCAGATGACAGTAGTGAAGAGTACCGTGGTCGTGCAAGATTTGATATTCTCCCTATTTCCTCCTCCTCCCCTGTCTTTTTTCTGGTCATTTTCCCAGCTTTTGTTGCAAATGTAGGAAGGGTAAAAGCTTGCATGAAAGAAAGGCGTCCTTTGAACGAGGAAGCTTATGAAATTTCTCTTGACCACTATGGGTGCATTGATGGTCCCAAATCTGGCAGGCTGAGGGTTTTAAGCAATGCACCCAAGCTCTTCATTGGCTTGAGTTTCTATTTTACTGGTGATTTTCCATCGGCTTATGAGGAGGATCTTCAAGATTTGGTCAAAGCTGCGGGAGGTACCATATTAGAGAAGGACGAATTCCCAGCACCTAGTTGTAATGACCAGACTGCACCAAAGGTGCTGGTTGTATACAATCTCGATTCTCCTGGAGGATGCAAAGTGGGGGTGGAGGTTTTAATTCTCTGGCAAAGATTAAATGAGGCAGAGGGAATAGCTGCAAAGGTTGGAGCCCAAGTCATTGGTCATACATGGCTTGTGGAATCCATTGCAGCGGGTAACTTGCAACATTTTGTCAGCTGCTAA

Coding sequence (CDS)

ATGCTAATAATAGCAGGTTTTTTGAACCTGTGTTGCTTCCTTGCACTCACCTGTTTTGCAAGGGTTGCATGCCATTTGCTTCCCAGATTGGATCAGTGTGCCCTCTCTGAAAATCAGGATTTGTCGATCGAATATGCTGGGAACAATTTACTTACCGAGAATAAAGAAAGACCAGTTGCAATTTTAATAGACGCTGGGGCAGCTGTGGACGAATCATGTTTTGGTCAGTCTATGCGTTACTCTTCCAGCAACTGCAAGGGGTTTGAGGGGTGCAGCATGCCTCTTGATTGTGAATGTGAGAATCTTGACATTGAGGGTAAAAAGGGTAGTGGGAGGCATGAAGATTGTAGAATGCCACCTGTGAGTCAGGCAAACCAGTTATCAGCCGGAAGCCCACCACACTTTGGTTCTGGCAAGGTATCAGATGACAGTAGTGAAGAGTACCGTGGTCGTGCAAGATTTGATATTCTCCCTATTTCCTCCTCCTCCCCTGTCTTTTTTCTGGTCATTTTCCCAGCTTTTGTTGCAAATGTAGGAAGGGTAAAAGCTTGCATGAAAGAAAGGCGTCCTTTGAACGAGGAAGCTTATGAAATTTCTCTTGACCACTATGGGTGCATTGATGGTCCCAAATCTGGCAGGCTGAGGGTTTTAAGCAATGCACCCAAGCTCTTCATTGGCTTGAGTTTCTATTTTACTGGTGATTTTCCATCGGCTTATGAGGAGGATCTTCAAGATTTGGTCAAAGCTGCGGGAGGTACCATATTAGAGAAGGACGAATTCCCAGCACCTAGTTGTAATGACCAGACTGCACCAAAGGTGCTGGTTGTATACAATCTCGATTCTCCTGGAGGATGCAAAGTGGGGGTGGAGGTTTTAATTCTCTGGCAAAGATTAAATGAGGCAGAGGGAATAGCTGCAAAGGTTGGAGCCCAAGTCATTGGTCATACATGGCTTGTGGAATCCATTGCAGCGGGTAACTTGCAACATTTTGTCAGCTGCTAA

Protein sequence

MLIIAGFLNLCCFLALTCFARVACHLLPRLDQCALSENQDLSIEYAGNNLLTENKERPVAILIDAGAAVDESCFGQSMRYSSSNCKGFEGCSMPLDCECENLDIEGKKGSGRHEDCRMPPVSQANQLSAGSPPHFGSGKVSDDSSEEYRGRARFDILPISSSSPVFFLVIFPAFVANVGRVKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTGDFPSAYEEDLQDLVKAAGGTILEKDEFPAPSCNDQTAPKVLVVYNLDSPGGCKVGVEVLILWQRLNEAEGIAAKVGAQVIGHTWLVESIAAGNLQHFVSC

Homology

[Close]

BLAST of CmaCh02G017300 vs. ExPASy Swiss-Prot
Match: F4I443 (BRCA1-associated RING domain protein 1 OS=Arabidopsis thaliana OX=3702 GN=BARD1 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.5e-41
Identity = 82/158 (51.90%), Postives = 111/158 (70.25%), Query Frame = 0


Query: 174 FVANVGRVKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTG 233
           ++ N   +KA +K  +P++EE +EI +D  GC DGPK+ RLR  +N PKLF GL FYF G
Sbjct: 553 WIINAAWMKASLKASQPVDEEPFEIQIDTQGCQDGPKTARLRAETNKPKLFEGLKFYFFG 612

Query: 234 DFPSAYEEDLQDLVKAAGGTILE-KDEFPAPS---CNDQTAPKVLVVYNLDSPGGCKVGV 293
           DF   Y+EDLQ+LVK AGGTIL  +DE  A S    NDQ +  + VVYN+D P GC +G 
Sbjct: 613 DFYKGYKEDLQNLVKVAGGTILNTEDELGAESSNNVNDQRSSSI-VVYNIDPPHGCALGE 672

Query: 294 EVLILWQRLNEAEGIAAKVGAQVIGHTWLVESIAAGNL 328
           EV I+WQR N+AE +A++ G++++GHTW++ESIA   L
Sbjct: 673 EVTIIWQRANDAEALASQTGSRLVGHTWVLESIAGYKL 709

[Close]

BLAST of CmaCh02G017300 vs. ExPASy Swiss-Prot
Match: Q8RXD4 (Protein BREAST CANCER SUSCEPTIBILITY 1 homolog OS=Arabidopsis thaliana OX=3702 GN=BRCA1 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.8e-23
Identity = 59/150 (39.33%), Postives = 87/150 (58.00%), Query Frame = 0


Query: 174 FVANVGRVKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTG 233
           ++  +  +KACMK  + ++EE YEI++D +G  +GP  GR R L   PKLF GL FY  G
Sbjct: 795 WILTIDWIKACMKNTKYVSEEPYEITMDVHGIREGPYLGRQRALKKKPKLFTGLKFYIMG 854

Query: 234 DFPSAYEEDLQDLVKAAGGTILEKDEFPAPSCNDQTAPKVLVVYNLDSPGGCKVGVEVLI 293
           DF  AY+  LQDL+ AAGGTIL +     P  +D      +VV++++ P   K       
Sbjct: 855 DFELAYKGYLQDLIVAAGGTILRR----RPVSSDDNEASTIVVFSVE-PSKKKT------ 914

Query: 294 LWQRLNEAEGIAAKVGAQVIGHTWLVESIA 324
           L QR ++AE +A    A+    +W+++SIA
Sbjct: 915 LTQRRSDAEALAKSARARAASSSWVLDSIA 933

[Close]

BLAST of CmaCh02G017300 vs. ExPASy Swiss-Prot
Match: Q9QZH2 (BRCA1-associated RING domain protein 1 OS=Rattus norvegicus OX=10116 GN=Bard1 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 8.1e-07
Identity = 42/147 (28.57%), Postives = 61/147 (41.50%), Query Frame = 0


Query: 181 VKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTGDFPSAYE 240
           VKAC+  +    EE YE+         GP+  RL      PKLF G  F+  G+F    +
Sbjct: 627 VKACLDSQEREQEEKYEVP-------GGPQRSRLNREQLLPKLFDGCYFFLGGNFKHHPK 686

Query: 241 EDLQDLVKAAGGTILEKDEFPAPSCNDQTAPKVLVVYNLDSPGGCKVGVEVLILWQRLNE 300
           EDL  L+ AAGG IL +   P P  +D T     V Y+       +   + ++     N 
Sbjct: 687 EDLLKLIAAAGGRILSRK--PKPD-SDVTQTINTVAYHAKPDSDQRFCTQYIVYEDLFNC 746

Query: 301 AEGIAAKVGAQVIGHTWLVESIAAGNL 328
                 +    +   TWL+  + A  L
Sbjct: 747 HPERVRQGKVWMAPSTWLISCVMAFEL 763

[Close]

BLAST of CmaCh02G017300 vs. ExPASy Swiss-Prot
Match: O70445 (BRCA1-associated RING domain protein 1 OS=Mus musculus OX=10090 GN=Bard1 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.0e-06
Identity = 41/147 (27.89%), Postives = 60/147 (40.82%), Query Frame = 0


Query: 181 VKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTGDFPSAYE 240
           VKAC+  +    EE YE+         GP+  RL      PKLF G  F+  G+F     
Sbjct: 624 VKACLDSKVREQEEKYEVP-------GGPQRSRLNREQLLPKLFDGCYFFLGGNFKHHPR 683

Query: 241 EDLQDLVKAAGGTILEKDEFPAPSCNDQTAPKVLVVYNLDSPGGCKVGVEVLILWQRLNE 300
           +DL  L+ AAGG +L +   P P  +D T     V Y+       +   + ++     N 
Sbjct: 684 DDLLKLIAAAGGKVLSRK--PKPD-SDVTQTINTVAYHAKPESDQRFCTQYIVYEDLFNC 743

Query: 301 AEGIAAKVGAQVIGHTWLVESIAAGNL 328
                 +    +   TWL+  I A  L
Sbjct: 744 HPERVRQGKVWMAPSTWLISCIMAFEL 760

[Close]

BLAST of CmaCh02G017300 vs. ExPASy Swiss-Prot
Match: Q99728 (BRCA1-associated RING domain protein 1 OS=Homo sapiens OX=9606 GN=BARD1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 6.8e-06
Identity = 31/81 (38.27%), Postives = 40/81 (49.38%), Query Frame = 0


Query: 181 VKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTGDFPSAYE 240
           VKAC++ +    EE YEI        +GP+  RL      PKLF G  FY  G F    +
Sbjct: 636 VKACLRRKVCEQEEKYEIP-------EGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPK 695

Query: 241 EDLQDLVKAAGGTILEKDEFP 262
           ++L  LV A GG IL +   P
Sbjct: 696 DNLIKLVTAGGGQILSRKPKP 709

[Close]

BLAST of CmaCh02G017300 vs. TAIR 10
Match: AT1G04020.2 (breast cancer associated RING 1 )

HSP 1 Score: 169.5 bits (428), Expect = 4.6e-42
Identity = 82/158 (51.90%), Postives = 111/158 (70.25%), Query Frame = 0


Query: 174 FVANVGRVKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTG 233
           ++ N   +KA +K  +P++EE +EI +D  GC DGPK+ RLR  +N PKLF GL FYF G
Sbjct: 552 WIINAAWMKASLKASQPVDEEPFEIQIDTQGCQDGPKTARLRAETNKPKLFEGLKFYFFG 611

Query: 234 DFPSAYEEDLQDLVKAAGGTILE-KDEFPAPS---CNDQTAPKVLVVYNLDSPGGCKVGV 293
           DF   Y+EDLQ+LVK AGGTIL  +DE  A S    NDQ +  + VVYN+D P GC +G 
Sbjct: 612 DFYKGYKEDLQNLVKVAGGTILNTEDELGAESSNNVNDQRSSSI-VVYNIDPPHGCALGE 671

Query: 294 EVLILWQRLNEAEGIAAKVGAQVIGHTWLVESIAAGNL 328
           EV I+WQR N+AE +A++ G++++GHTW++ESIA   L
Sbjct: 672 EVTIIWQRANDAEALASQTGSRLVGHTWVLESIAGYKL 708

[Close]

BLAST of CmaCh02G017300 vs. TAIR 10
Match: AT1G04020.1 (breast cancer associated RING 1 )

HSP 1 Score: 169.5 bits (428), Expect = 4.6e-42
Identity = 82/158 (51.90%), Postives = 111/158 (70.25%), Query Frame = 0


Query: 174 FVANVGRVKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTG 233
           ++ N   +KA +K  +P++EE +EI +D  GC DGPK+ RLR  +N PKLF GL FYF G
Sbjct: 553 WIINAAWMKASLKASQPVDEEPFEIQIDTQGCQDGPKTARLRAETNKPKLFEGLKFYFFG 612

Query: 234 DFPSAYEEDLQDLVKAAGGTILE-KDEFPAPS---CNDQTAPKVLVVYNLDSPGGCKVGV 293
           DF   Y+EDLQ+LVK AGGTIL  +DE  A S    NDQ +  + VVYN+D P GC +G 
Sbjct: 613 DFYKGYKEDLQNLVKVAGGTILNTEDELGAESSNNVNDQRSSSI-VVYNIDPPHGCALGE 672

Query: 294 EVLILWQRLNEAEGIAAKVGAQVIGHTWLVESIAAGNL 328
           EV I+WQR N+AE +A++ G++++GHTW++ESIA   L
Sbjct: 673 EVTIIWQRANDAEALASQTGSRLVGHTWVLESIAGYKL 709

[Close]

BLAST of CmaCh02G017300 vs. TAIR 10
Match: AT4G21070.1 (breast cancer susceptibility1 )

HSP 1 Score: 110.9 bits (276), Expect = 2.0e-24
Identity = 59/150 (39.33%), Postives = 87/150 (58.00%), Query Frame = 0


Query: 174 FVANVGRVKACMKERRPLNEEAYEISLDHYGCIDGPKSGRLRVLSNAPKLFIGLSFYFTG 233
           ++  +  +KACMK  + ++EE YEI++D +G  +GP  GR R L   PKLF GL FY  G
Sbjct: 795 WILTIDWIKACMKNTKYVSEEPYEITMDVHGIREGPYLGRQRALKKKPKLFTGLKFYIMG 854

Query: 234 DFPSAYEEDLQDLVKAAGGTILEKDEFPAPSCNDQTAPKVLVVYNLDSPGGCKVGVEVLI 293
           DF  AY+  LQDL+ AAGGTIL +     P  +D      +VV++++ P   K       
Sbjct: 855 DFELAYKGYLQDLIVAAGGTILRR----RPVSSDDNEASTIVVFSVE-PSKKKT------ 914

Query: 294 LWQRLNEAEGIAAKVGAQVIGHTWLVESIA 324
           L QR ++AE +A    A+    +W+++SIA
Sbjct: 915 LTQRRSDAEALAKSARARAASSSWVLDSIA 933

The following BLAST results are available for this feature:

BLAST of CmaCh02G017300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I443	6.5e-41	51.90	BRCA1-associated RING domain protein 1 OS=Arabidopsis thaliana OX=3702 GN=BARD1 ...	[more]
Q8RXD4	2.8e-23	39.33	Protein BREAST CANCER SUSCEPTIBILITY 1 homolog OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9QZH2	8.1e-07	28.57	BRCA1-associated RING domain protein 1 OS=Rattus norvegicus OX=10116 GN=Bard1 PE...	[more]
O70445	4.0e-06	27.89	BRCA1-associated RING domain protein 1 OS=Mus musculus OX=10090 GN=Bard1 PE=2 SV...	[more]
Q99728	6.8e-06	38.27	BRCA1-associated RING domain protein 1 OS=Homo sapiens OX=9606 GN=BARD1 PE=1 SV=...	[more]

BLAST of CmaCh02G017300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04020.2	4.6e-42	51.90	breast cancer associated RING 1	[more]
AT1G04020.1	4.6e-42	51.90	breast cancer associated RING 1	[more]
AT4G21070.1	2.0e-24	39.33	breast cancer susceptibility1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001357	BRCT domain	SMART	SM00292	BRCT_7	coord: 221..325 e-value: 6.1E-6 score: 35.7
IPR001357	BRCT domain	PROSITE	PS50172	BRCT	coord: 219..327 score: 13.423059
IPR036420	BRCT domain superfamily	GENE3D	3.40.50.10190	BRCT domain	coord: 220..324 e-value: 4.5E-21 score: 77.4
IPR036420	BRCT domain superfamily	SUPERFAMILY	52113	BRCT domain	coord: 223..329
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 110..142
None	No IPR available	PANTHER	PTHR13763:SF9	BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1	coord: 175..329
IPR031099	BRCA1-associated	PANTHER	PTHR13763	BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN BRCA1	coord: 175..329

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G017300.1	CmaCh02G017300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0006974	cellular response to DNA damage stimulus
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0046872	metal ion binding