CmaCh02G015660 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G015660
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	phospholipase A1-Ibeta2, chloroplastic-like
Location	Cma_Chr02: 8886053 .. 8887546 (+)
RNA-Seq Expression	CmaCh02G015660
Synteny	CmaCh02G015660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACTAATCACCTCCACAATTCCGCCCCAAAATCTCACCGGCTTTCAGGTCTGCCAGTCCAGTTTCAAGTACCAAATCTCGCCGTTGAACCCGTCGGCGGCCAAGATTTCTAATTCCCGTGAGACGGCGGCGCCGTTTGATGTCACTCGCCGGCATCTGGCAAACTTGGATAAGTTGCTAAAGAAAACCGATCCGCCTACTATGTTGGAGCCGGAACCGGTTCACAAGGACCCCAACAAGGGATCCATACCCATTAAAGGGAAGGGGCTTTTACAACACTTGAACCTAGCTAGAATCTGGCCAGGGAGGAAGGCGGCTGAGGACATGTCGCCGAAAAACCTCAACCGGCTCCACCAGTTACTCTCGAAAACGGTGGAGTATTCACCAAAAAGCTCCCTAGGTCAGCGGTGGCGCGAGTACCACGGCTGCAACAATTGGTCCGGTTTACTCGACCCGCTTGACGAAAATCTACGCCGTGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCATATCAGTCTTTTTATTCAAACCCCGCCACGTCACCCAACGAGGCCCCACTTCCTCACCACGTGGCGCTTTTAGATAGGTCTTATAAAGTTACAAAAAGTCTCTATGCCACGGCATCAGTTGGTCTACCCGGTTGGGTGGATGAGGTAGCTCCGGATATCAACTGGATGACCCAGCGGTCCAGTTGGGTTGGATACGTAGCCGTTTGCGACGACCGCCGTGAAATCGCCCGTATGGGGCGGCGCGATATCGTCATCGCCCTCCGTGGCACCGCGACATGTCTTGAATGGGCCGAAAATTTCAGAGCTTATCAAATCAAAATCCCCGCCGACAACAACACCACCGATGAAGATTCAAAAGTCGAGTGCGGATTTCTCAGCCTATACAAAACCGCCGGAGCCCATGTCAAAAGCTTATCGGAATCAGTGGTGGAGGAAATTAAACGATTAACAGAGCTCTACAGAGGAGAAACATTAAGTATAACCGTAACCGGACACAGTTTGGGTGCAGCATTGGCTGTACTGATGGCAGATGAGATCAGTGTTTGTTCGCCGGAAGTTCCTCCGGTGGCGGTGTTTTCATTCGGCGGGCCGCGCGTGGGAAACAAAAGCTTTGCAGATCGAATTGAATCAAGAAACGTGAAAGTATTGAGAATTGTGAACTCACAAGACGTGATAACGCAAGTTCCACCGGTGACGTATTCACATGTCGGAACAGAGCTGCGAGTGGATACAAAAATGTCGCCATACCTTAAACCCAACGCTGACGTGGCTTGCTGCCATGATTTAGAGGCGTATTTGCATTTAGTGGACGGATTCTTGTCGTCCAAATGTCCGTTCAGGCCAAATGCAAAACGGAGTTTGGCGCGATTAATGCAAGACCAGAAGGGGAATGTGAAGAAATTGTACATGAGTAAGGTGAAGGATTTGGGGCTGAATCCAGAGCTGCAAACCTCAGGGTGCTTGCCTAGTCCCTCCTGA

mRNA sequence

ATGCAACTAATCACCTCCACAATTCCGCCCCAAAATCTCACCGGCTTTCAGGTCTGCCAGTCCAGTTTCAAGTACCAAATCTCGCCGTTGAACCCGTCGGCGGCCAAGATTTCTAATTCCCGTGAGACGGCGGCGCCGTTTGATGTCACTCGCCGGCATCTGGCAAACTTGGATAAGTTGCTAAAGAAAACCGATCCGCCTACTATGTTGGAGCCGGAACCGGTTCACAAGGACCCCAACAAGGGATCCATACCCATTAAAGGGAAGGGGCTTTTACAACACTTGAACCTAGCTAGAATCTGGCCAGGGAGGAAGGCGGCTGAGGACATGTCGCCGAAAAACCTCAACCGGCTCCACCAGTTACTCTCGAAAACGGTGGAGTATTCACCAAAAAGCTCCCTAGGTCAGCGGTGGCGCGAGTACCACGGCTGCAACAATTGGTCCGGTTTACTCGACCCGCTTGACGAAAATCTACGCCGTGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCATATCAGTCTTTTTATTCAAACCCCGCCACGTCACCCAACGAGGCCCCACTTCCTCACCACGTGGCGCTTTTAGATAGGTCTTATAAAGTTACAAAAAGTCTCTATGCCACGGCATCAGTTGGTCTACCCGGTTGGGTGGATGAGGTAGCTCCGGATATCAACTGGATGACCCAGCGGTCCAGTTGGGTTGGATACGTAGCCGTTTGCGACGACCGCCGTGAAATCGCCCGTATGGGGCGGCGCGATATCGTCATCGCCCTCCGTGGCACCGCGACATGTCTTGAATGGGCCGAAAATTTCAGAGCTTATCAAATCAAAATCCCCGCCGACAACAACACCACCGATGAAGATTCAAAAGTCGAGTGCGGATTTCTCAGCCTATACAAAACCGCCGGAGCCCATGTCAAAAGCTTATCGGAATCAGTGGTGGAGGAAATTAAACGATTAACAGAGCTCTACAGAGGAGAAACATTAAGTATAACCGTAACCGGACACAGTTTGGGTGCAGCATTGGCTGTACTGATGGCAGATGAGATCAGTGTTTGTTCGCCGGAAGTTCCTCCGGTGGCGGTGTTTTCATTCGGCGGGCCGCGCGTGGGAAACAAAAGCTTTGCAGATCGAATTGAATCAAGAAACGTGAAAGTATTGAGAATTGTGAACTCACAAGACGTGATAACGCAAGTTCCACCGGTGACGTATTCACATGTCGGAACAGAGCTGCGAGTGGATACAAAAATGTCGCCATACCTTAAACCCAACGCTGACGTGGCTTGCTGCCATGATTTAGAGGCGTATTTGCATTTAGTGGACGGATTCTTGTCGTCCAAATGTCCGTTCAGGCCAAATGCAAAACGGAGTTTGGCGCGATTAATGCAAGACCAGAAGGGGAATGTGAAGAAATTGTACATGAGTAAGGTGAAGGATTTGGGGCTGAATCCAGAGCTGCAAACCTCAGGGTGCTTGCCTAGTCCCTCCTGA

Coding sequence (CDS)

ATGCAACTAATCACCTCCACAATTCCGCCCCAAAATCTCACCGGCTTTCAGGTCTGCCAGTCCAGTTTCAAGTACCAAATCTCGCCGTTGAACCCGTCGGCGGCCAAGATTTCTAATTCCCGTGAGACGGCGGCGCCGTTTGATGTCACTCGCCGGCATCTGGCAAACTTGGATAAGTTGCTAAAGAAAACCGATCCGCCTACTATGTTGGAGCCGGAACCGGTTCACAAGGACCCCAACAAGGGATCCATACCCATTAAAGGGAAGGGGCTTTTACAACACTTGAACCTAGCTAGAATCTGGCCAGGGAGGAAGGCGGCTGAGGACATGTCGCCGAAAAACCTCAACCGGCTCCACCAGTTACTCTCGAAAACGGTGGAGTATTCACCAAAAAGCTCCCTAGGTCAGCGGTGGCGCGAGTACCACGGCTGCAACAATTGGTCCGGTTTACTCGACCCGCTTGACGAAAATCTACGCCGTGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCATATCAGTCTTTTTATTCAAACCCCGCCACGTCACCCAACGAGGCCCCACTTCCTCACCACGTGGCGCTTTTAGATAGGTCTTATAAAGTTACAAAAAGTCTCTATGCCACGGCATCAGTTGGTCTACCCGGTTGGGTGGATGAGGTAGCTCCGGATATCAACTGGATGACCCAGCGGTCCAGTTGGGTTGGATACGTAGCCGTTTGCGACGACCGCCGTGAAATCGCCCGTATGGGGCGGCGCGATATCGTCATCGCCCTCCGTGGCACCGCGACATGTCTTGAATGGGCCGAAAATTTCAGAGCTTATCAAATCAAAATCCCCGCCGACAACAACACCACCGATGAAGATTCAAAAGTCGAGTGCGGATTTCTCAGCCTATACAAAACCGCCGGAGCCCATGTCAAAAGCTTATCGGAATCAGTGGTGGAGGAAATTAAACGATTAACAGAGCTCTACAGAGGAGAAACATTAAGTATAACCGTAACCGGACACAGTTTGGGTGCAGCATTGGCTGTACTGATGGCAGATGAGATCAGTGTTTGTTCGCCGGAAGTTCCTCCGGTGGCGGTGTTTTCATTCGGCGGGCCGCGCGTGGGAAACAAAAGCTTTGCAGATCGAATTGAATCAAGAAACGTGAAAGTATTGAGAATTGTGAACTCACAAGACGTGATAACGCAAGTTCCACCGGTGACGTATTCACATGTCGGAACAGAGCTGCGAGTGGATACAAAAATGTCGCCATACCTTAAACCCAACGCTGACGTGGCTTGCTGCCATGATTTAGAGGCGTATTTGCATTTAGTGGACGGATTCTTGTCGTCCAAATGTCCGTTCAGGCCAAATGCAAAACGGAGTTTGGCGCGATTAATGCAAGACCAGAAGGGGAATGTGAAGAAATTGTACATGAGTAAGGTGAAGGATTTGGGGCTGAATCCAGAGCTGCAAACCTCAGGGTGCTTGCCTAGTCCCTCCTGA

Protein sequence

MQLITSTIPPQNLTGFQVCQSSFKYQISPLNPSAAKISNSRETAAPFDVTRRHLANLDKLLKKTDPPTMLEPEPVHKDPNKGSIPIKGKGLLQHLNLARIWPGRKAAEDMSPKNLNRLHQLLSKTVEYSPKSSLGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPATSPNEAPLPHHVALLDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCDDRREIARMGRRDIVIALRGTATCLEWAENFRAYQIKIPADNNTTDEDSKVECGFLSLYKTAGAHVKSLSESVVEEIKRLTELYRGETLSITVTGHSLGAALAVLMADEISVCSPEVPPVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVPPVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLMQDQKGNVKKLYMSKVKDLGLNPELQTSGCLPSPS

Homology

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BLAST of CmaCh02G015660 vs. ExPASy Swiss-Prot
Match: O23522 (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 535.8 bits (1379), Expect = 5.2e-151
Identity = 293/504 (58.13%), Postives = 347/504 (68.85%), Query Frame = 0


Query: 29  PLNPSAAKISNSRETAAPFDVTRRHLANLDKLLKKTDPPTMLEPEPVHKDPNKGSIPIKG 88
           PL+ S A+ +N           + HL NL+ +L +T   ++   E V     K +     
Sbjct: 32  PLSVSPARKTN-----------KEHLRNLENVL-RTSSNSIDHIENVTSRQEKTTKNTST 91

Query: 89  KGLLQHLNLARIWPGRKAA-EDMSPKNLNRLHQLLSK-TVEYSPKSSLGQRWREYHGCNN 148
             LL  LNLARIWP  KAA ++MSPKNL RL +LLSK + E SPKS LG +WRE HG NN
Sbjct: 92  SSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEERSPKSKLGSKWRELHGLNN 151

Query: 149 WSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPATSPNEAPLPHHVALLDRSYKVTKSL 208
           W+GLLDPLDENLRRE+VRYGEFVQAAY +F+S+P  S      P HVAL D S+KVTKSL
Sbjct: 152 WAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGS------PRHVALPDGSFKVTKSL 211

Query: 209 YATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCDDRREIARMGRRDIVIALRGTATCL 268
           YAT+SV LP W+D+VAPD+ WMT+++SWVGYVAVCDD REI RMGRR+IVIALRGTAT L
Sbjct: 212 YATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIRRMGRREIVIALRGTATLL 271

Query: 269 EWAENFRAYQIKIPADNNTTDEDS--KVECGFLSLYKTAGAHVKSLSESVVEEIKRLTEL 328
           EW+ENFR   + +P       + +  KVECGF SLY T   H  SL+ES+V EI RL EL
Sbjct: 272 EWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTTGDQHAPSLAESLVGEISRLVEL 331

Query: 329 YRGETLSITVTGHSLGAALAVLMADEISVCSPEVPPVAVFSFGGPRVGNKSFADRIESRN 388
           Y GE LSI+VTGHSLGAA+A+L AD+I+   P  PPVAVFSFGGPRVGN+ FADR++S+ 
Sbjct: 332 YAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFSFGGPRVGNREFADRLDSKG 391

Query: 389 VKVLRIVNSQDVITQVP------------------------------PVTYSHVGTELRV 448
           VKVLR+VNSQDV+T+VP                              P  YSHVG ELRV
Sbjct: 392 VKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIMEMVERNNPWAYSHVGAELRV 451

Query: 449 DTKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLMQDQKGNVKKLY 498
           D KMSPYLKPNADVACCHDLEAYLHLVDGFL+S CPFR NAKRSL +L+ +Q+ NVK LY
Sbjct: 452 DMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANAKRSLRKLLDEQRSNVKVLY 511

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BLAST of CmaCh02G015660 vs. ExPASy Swiss-Prot
Match: Q948R1 (Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.9e-89
Identity = 184/381 (48.29%), Postives = 238/381 (62.47%), Query Frame = 0


Query: 127 EYSPKSS-----LGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPA 186
           E+SP  S     LG+RW EY G  NW GLLDPLD+NLRRE++RYG+FV++AYQ+F  +P+
Sbjct: 74  EFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPS 133

Query: 187 TSPNEAPLPHHVALLDRS------YKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWV 246
           +            LL+RS      Y++TK+L AT+ + LP W+ E AP  +WM  +SSW+
Sbjct: 134 SPTYGTCRFPRSTLLERSGLPNSGYRLTKNLRATSGINLPRWI-EKAP--SWMATQSSWI 193

Query: 247 GYVAVCDDRREIARMGRRDIVIALRGTATCLEWAENFRAYQIKIP-----ADNNTTDEDS 306
           GYVAVC D+ EI+R+GRRD+VI+ RGTATCLEW EN RA    +P     A+ N ++   
Sbjct: 194 GYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGP 253

Query: 307 KVECGFLSLYKTAGAHVKSLSESVVEEIKRLTELYRGETLSITVTGHSLGAALAVLMADE 366
            VE GFLSLY T+G H  SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A +
Sbjct: 254 MVESGFLSLY-TSGVH--SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYD 313

Query: 367 ISVCSPEVPPVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP--------- 426
           I       P V V SFGGPRVGN+ F   +E +  KVLRIVNS DVIT+VP         
Sbjct: 314 IKTTFKRAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQ 373

Query: 427 ---------------------PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHL 462
                                P  Y+ +G ELR+ ++ SP+L  + +VA CH+L+ YLHL
Sbjct: 374 DNVKMTASIMPSWIQRRVEETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLHL 433

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BLAST of CmaCh02G015660 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.1e-67
Identity = 154/362 (42.54%), Postives = 204/362 (56.35%), Query Frame = 0


Query: 127 EYSPKSSLGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPAT---- 186
           E   + SL + WRE  GCNNW G LDP++ +LRRE++RYGEF QA Y SF  +P +    
Sbjct: 80  EEEKEVSLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCG 139

Query: 187 SPNEAPLPHHVAL---LDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVA 246
           S    P    + L   L + Y +T+ LYAT+++ LP +  +      W +Q ++W+G+VA
Sbjct: 140 SCKYHPSDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVA 199

Query: 247 VCDDRREIARMGRRDIVIALRGTATCLEWAENFRAYQIK--IPADNNTTDEDSKVECGFL 306
           V  D  E++R+GRRDIVIA RGT T LEW      Y +K  + + N   D   K+E GF 
Sbjct: 200 VATDEEEVSRLGRRDIVIAWRGTVTYLEW-----IYDLKDILCSANFGDDPSIKIELGFH 259

Query: 307 SLYKTAGAHVK----SLSESVVEEIKRLTELY----RGETLSITVTGHSLGAALAVLMAD 366
            LY       K    S  E V+ E+KRL E Y     G   SITVTGHSLGA+LA++ A 
Sbjct: 260 DLYTKKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAY 319

Query: 367 EISVCS-PEVP------PVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP- 426
           +I+  +   VP      P+ VFSF GPRVGN  F +R +   VKVLR+VN  D +  VP 
Sbjct: 320 DIAELNLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPG 379

Query: 427 -------------------PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVD 445
                              P +Y+HVG EL +D K SP+LKP  D+ C H+LEA LHLVD
Sbjct: 380 IFTNEKFQFQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVD 435

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BLAST of CmaCh02G015660 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 238.8 bits (608), Expect = 1.3e-61
Identity = 141/351 (40.17%), Postives = 197/351 (56.13%), Query Frame = 0


Query: 134 LGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPATSP------NEA 193
           L   WR+  G ++W+GL+DP+D  LR E++RYGE  QA Y +F  +PA+           
Sbjct: 101 LRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRL 160

Query: 194 PLPHHVALLDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCDDRREI 253
                + ++D  Y+V + LYAT+++ LP +  +      W ++ ++W+GYVAV DD    
Sbjct: 161 EFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSR 220

Query: 254 ARMGRRDIVIALRGTATCLEWAENFRAYQIKIPADN--NTTDEDSKVECGFLSLYKTAGA 313
            R+GRRDI IA RGT T LEW  + + Y +K   +N     D   KVE GFL LY     
Sbjct: 221 NRLGRRDIAIAWRGTVTKLEWIADLKDY-LKPVTENKIRCPDPAVKVESGFLDLYTDKDT 280

Query: 314 HVK----SLSESVVEEIKRLTELYRGE---TLSITVTGHSLGAALAVLMADEISVC---- 373
             K    S  E ++ E+KRL E +  +    LSITVTGHSLG ALA+L A +I+      
Sbjct: 281 TCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNR 340

Query: 374 --SPEVPPVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP----------- 433
               +V PV V ++GGPRVGN  F +R+E   VKV+R+VN  DV+ + P           
Sbjct: 341 SKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHA 400

Query: 434 --------PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVDGF 445
                   P  YSHVG EL +D + SP+LKP+ DV+  H+LEA LHL+DG+
Sbjct: 401 LMKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGY 449

[Close]

BLAST of CmaCh02G015660 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 236.9 bits (603), Expect = 5.0e-61
Identity = 142/358 (39.66%), Postives = 195/358 (54.47%), Query Frame = 0


Query: 127 EYSPKSSLGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNP------ 186
           E      L   WR+  G ++W+GL+DP+D  LR E++RYGE  QA Y +F  +P      
Sbjct: 78  EAKESKRLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCG 137

Query: 187 ATSPNEAPLPHHVALLDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVAV 246
           +       L   + ++D  Y+V + LYAT+++ LP +  +      W ++ ++W+GYVAV
Sbjct: 138 SCRFTRRHLFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAV 197

Query: 247 CDDRREI-ARMGRRDIVIALRGTATCLEWAENFRAYQIKIPADN-NTTDEDSKVECGFLS 306
            DD      R+GRRDI IA RGT T LEW  + + +   +  +     D   K E GFL 
Sbjct: 198 SDDNEATRCRLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLD 257

Query: 307 LY--KTAGAHVKSLS--ESVVEEIKRLTELY---RGETLSITVTGHSLGAALAVLMADEI 366
           LY  K    +    S  E V+ E+KRL E Y    GE LSITVTGHSLG ALAVL A ++
Sbjct: 258 LYTDKDTSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDV 317

Query: 367 S------VCSPEVPPVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP---- 426
           +          +V PV  F++GGPRVGN  F +RIE   VKVLR+VN  DV+ + P    
Sbjct: 318 AEMGVNRTRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFL 377

Query: 427 ---------------PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVDGF 445
                          P  YSHVG  L +D + SP+LKP  D++  H+LEA LHL+DG+
Sbjct: 378 NERAPQALMKLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGY 434

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BLAST of CmaCh02G015660 vs. TAIR 10
Match: AT4G16820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 535.8 bits (1379), Expect = 3.7e-152
Identity = 293/504 (58.13%), Postives = 347/504 (68.85%), Query Frame = 0


Query: 29  PLNPSAAKISNSRETAAPFDVTRRHLANLDKLLKKTDPPTMLEPEPVHKDPNKGSIPIKG 88
           PL+ S A+ +N           + HL NL+ +L +T   ++   E V     K +     
Sbjct: 32  PLSVSPARKTN-----------KEHLRNLENVL-RTSSNSIDHIENVTSRQEKTTKNTST 91

Query: 89  KGLLQHLNLARIWPGRKAA-EDMSPKNLNRLHQLLSK-TVEYSPKSSLGQRWREYHGCNN 148
             LL  LNLARIWP  KAA ++MSPKNL RL +LLSK + E SPKS LG +WRE HG NN
Sbjct: 92  SSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEERSPKSKLGSKWRELHGLNN 151

Query: 149 WSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPATSPNEAPLPHHVALLDRSYKVTKSL 208
           W+GLLDPLDENLRRE+VRYGEFVQAAY +F+S+P  S      P HVAL D S+KVTKSL
Sbjct: 152 WAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGS------PRHVALPDGSFKVTKSL 211

Query: 209 YATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCDDRREIARMGRRDIVIALRGTATCL 268
           YAT+SV LP W+D+VAPD+ WMT+++SWVGYVAVCDD REI RMGRR+IVIALRGTAT L
Sbjct: 212 YATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIRRMGRREIVIALRGTATLL 271

Query: 269 EWAENFRAYQIKIPADNNTTDEDS--KVECGFLSLYKTAGAHVKSLSESVVEEIKRLTEL 328
           EW+ENFR   + +P       + +  KVECGF SLY T   H  SL+ES+V EI RL EL
Sbjct: 272 EWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTTGDQHAPSLAESLVGEISRLVEL 331

Query: 329 YRGETLSITVTGHSLGAALAVLMADEISVCSPEVPPVAVFSFGGPRVGNKSFADRIESRN 388
           Y GE LSI+VTGHSLGAA+A+L AD+I+   P  PPVAVFSFGGPRVGN+ FADR++S+ 
Sbjct: 332 YAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFSFGGPRVGNREFADRLDSKG 391

Query: 389 VKVLRIVNSQDVITQVP------------------------------PVTYSHVGTELRV 448
           VKVLR+VNSQDV+T+VP                              P  YSHVG ELRV
Sbjct: 392 VKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIMEMVERNNPWAYSHVGAELRV 451

Query: 449 DTKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLMQDQKGNVKKLY 498
           D KMSPYLKPNADVACCHDLEAYLHLVDGFL+S CPFR NAKRSL +L+ +Q+ NVK LY
Sbjct: 452 DMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANAKRSLRKLLDEQRSNVKVLY 511

[Close]

BLAST of CmaCh02G015660 vs. TAIR 10
Match: AT2G44810.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 7.1e-87
Identity = 176/363 (48.48%), Postives = 228/363 (62.81%), Query Frame = 0


Query: 140 EYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPATSPNEAPLPHHVALLDRS 199
           EY G  NW GLLDPLD+NLRRE++RYG+FV++AYQ+F  +P++            LL+RS
Sbjct: 2   EYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERS 61

Query: 200 ------YKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCDDRREIARMGRR 259
                 Y++TK+L AT+ + LP W+ E AP  +WM  +SSW+GYVAVC D+ EI+R+GRR
Sbjct: 62  GLPNSGYRLTKNLRATSGINLPRWI-EKAP--SWMATQSSWIGYVAVCQDKEEISRLGRR 121

Query: 260 DIVIALRGTATCLEWAENFRAYQIKIP-----ADNNTTDEDSKVECGFLSLYKTAGAHVK 319
           D+VI+ RGTATCLEW EN RA    +P     A+ N ++    VE GFLSLY T+G H  
Sbjct: 122 DVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLY-TSGVH-- 181

Query: 320 SLSESVVEEIKRLTELYRGETLSITVTGHSLGAALAVLMADEISVCSPEVPPVAVFSFGG 379
           SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A +I       P V V SFGG
Sbjct: 182 SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYDIKTTFKRAPMVTVISFGG 241

Query: 380 PRVGNKSFADRIESRNVKVLRIVNSQDVITQVP--------------------------- 439
           PRVGN+ F   +E +  KVLRIVNS DVIT+VP                           
Sbjct: 242 PRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRRV 301

Query: 440 ---PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRS 462
              P  Y+ +G ELR+ ++ SP+L  + +VA CH+L+ YLHLVDGF+SS CPFR  A+R 
Sbjct: 302 EETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLHLVDGFVSSTCPFRETARRV 357

[Close]

BLAST of CmaCh02G015660 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 3.5e-70
Identity = 149/363 (41.05%), Postives = 211/363 (58.13%), Query Frame = 0


Query: 129 SPKSSLGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNP------AT 188
           SP+  + + WRE HG NNW  LLDPL   LRREV +YGEFV++ Y S   +P      ++
Sbjct: 109 SPREKISKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSS 168

Query: 189 SPNEAPLPHHVALLDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCD 248
             N   L   + L    YKVTK +YA + V +P W    A    W ++ S+W+G+VAV  
Sbjct: 169 RYNRNKLFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETW-SKDSNWMGFVAVSG 228

Query: 249 DRREIARMGRRDIVIALRGTATCLEWAENFRAYQIKIPADNNTTDEDSKVECGFLSLYKT 308
           D RE  R+GRRDIV+A RGT T  EW  + R        +        KV+ GFLS+Y +
Sbjct: 229 D-RESLRIGRRDIVVAWRGTVTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSIYNS 288

Query: 309 AGA----HVKSLSESVVEEIKRLTELY--RGETLSITVTGHSLGAALAVLMADEISVCSP 368
                  + +S SE  ++E+KRL   +  RGE +S+T+TGHSLG ALA++ A E +   P
Sbjct: 289 KSELTRYNKESASEQTMDEVKRLVNFFKDRGEEVSLTITGHSLGGALALMNAYEAARDVP 348

Query: 369 EVP-PVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP------------PV 428
            +   ++V SFG PRVGN +F +++ S  VKVLR+VN QD++ ++P            P+
Sbjct: 349 ALSGNISVISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNKLNPI 408

Query: 429 T------YSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKR 461
           T      Y HVGT+L++D   SPY+K ++D+   H+LE YLH++DGF   K  FR NA+R
Sbjct: 409 TSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRVNARR 468

[Close]

BLAST of CmaCh02G015660 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.5e-68
Identity = 154/362 (42.54%), Postives = 204/362 (56.35%), Query Frame = 0


Query: 127 EYSPKSSLGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPAT---- 186
           E   + SL + WRE  GCNNW G LDP++ +LRRE++RYGEF QA Y SF  +P +    
Sbjct: 80  EEEKEVSLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCG 139

Query: 187 SPNEAPLPHHVAL---LDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVA 246
           S    P    + L   L + Y +T+ LYAT+++ LP +  +      W +Q ++W+G+VA
Sbjct: 140 SCKYHPSDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVA 199

Query: 247 VCDDRREIARMGRRDIVIALRGTATCLEWAENFRAYQIK--IPADNNTTDEDSKVECGFL 306
           V  D  E++R+GRRDIVIA RGT T LEW      Y +K  + + N   D   K+E GF 
Sbjct: 200 VATDEEEVSRLGRRDIVIAWRGTVTYLEW-----IYDLKDILCSANFGDDPSIKIELGFH 259

Query: 307 SLYKTAGAHVK----SLSESVVEEIKRLTELY----RGETLSITVTGHSLGAALAVLMAD 366
            LY       K    S  E V+ E+KRL E Y     G   SITVTGHSLGA+LA++ A 
Sbjct: 260 DLYTKKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAY 319

Query: 367 EISVCS-PEVP------PVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP- 426
           +I+  +   VP      P+ VFSF GPRVGN  F +R +   VKVLR+VN  D +  VP 
Sbjct: 320 DIAELNLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPG 379

Query: 427 -------------------PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVD 445
                              P +Y+HVG EL +D K SP+LKP  D+ C H+LEA LHLVD
Sbjct: 380 IFTNEKFQFQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVD 435

[Close]

BLAST of CmaCh02G015660 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 2.4e-63
Identity = 142/353 (40.23%), Postives = 199/353 (56.37%), Query Frame = 0


Query: 134 LGQRWREYHGCNNWSGLLDPLDENLRREVVRYGEFVQAAYQSFYSNPATSP------NEA 193
           L   WR+  G ++W+GL+DP+D  LR E++RYGE  QA Y +F  +PA+           
Sbjct: 101 LRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRL 160

Query: 194 PLPHHVALLDRSYKVTKSLYATASVGLPGWVDEVAPDINWMTQRSSWVGYVAVCDDRREI 253
                + ++D  Y+V + LYAT+++ LP +  +      W ++ ++W+GYVAV DD    
Sbjct: 161 EFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSR 220

Query: 254 ARMGRRDIVIALRGTATCLEWAENFRAYQIKIPADN--NTTDEDSKVECGFLSLYKTAGA 313
            R+GRRDI IA RGT T LEW  + + Y +K   +N     D   KVE GFL LY     
Sbjct: 221 NRLGRRDIAIAWRGTVTKLEWIADLKDY-LKPVTENKIRCPDPAVKVESGFLDLYTDKDT 280

Query: 314 HVK----SLSESVVEEIKRLTELYRGE---TLSITVTGHSLGAALAVLMADEISVC---- 373
             K    S  E ++ E+KRL E +  +    LSITVTGHSLG ALA+L A +I+      
Sbjct: 281 TCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNR 340

Query: 374 --SPEVPPVAVFSFGGPRVGNKSFADRIESRNVKVLRIVNSQDVITQVP----------- 433
               +V PV V ++GGPRVGN  F +R+E   VKV+R+VN  DV+ + P           
Sbjct: 341 SKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHA 400

Query: 434 --------PVTYSHVGTELRVDTKMSPYLKPNADVACCHDLEAYLHLVDGFLS 447
                   P  YSHVG EL +D + SP+LKP+ DV+  H+LEA LHL+DG++S
Sbjct: 401 LMKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

The following BLAST results are available for this feature:

BLAST of CmaCh02G015660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23522	5.2e-151	58.13	Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16...	[more]
Q948R1	1.9e-89	48.29	Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 P...	[more]
Q9C8J6	2.1e-67	42.54	Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2...	[more]
Q3EBR6	1.3e-61	40.17	Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3...	[more]
Q941F1	5.0e-61	39.66	Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4...	[more]

BLAST of CmaCh02G015660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16820.1	3.7e-152	58.13	alpha/beta-Hydrolases superfamily protein	[more]
AT2G44810.1	7.1e-87	48.48	alpha/beta-Hydrolases superfamily protein	[more]
AT1G30370.1	3.5e-70	41.05	alpha/beta-Hydrolases superfamily protein	[more]
AT1G51440.1	1.5e-68	42.54	alpha/beta-Hydrolases superfamily protein	[more]
AT2G30550.1	2.4e-63	40.23	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 137..480 e-value: 1.3E-103 score: 349.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 158..444
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 256..403 e-value: 3.2E-36 score: 124.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 64..86
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 64..83
None	No IPR available	PANTHER	PTHR31403:SF37	GLYCEROLIPASE A1	coord: 1..402
None	No IPR available	PANTHER	PTHR31403	PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC	coord: 402..485
None	No IPR available	PANTHER	PTHR31403:SF37	GLYCEROLIPASE A1	coord: 402..485
None	No IPR available	PANTHER	PTHR31403	PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC	coord: 1..402
None	No IPR available	CDD	cd00519	Lipase_3	coord: 189..442 e-value: 3.33107E-49 score: 166.883

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G015660.1	CmaCh02G015660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0004620	phospholipase activity