CmaCh02G015470 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G015470
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein NRT1/ PTR FAMILY 6.3-like
Location	Cma_Chr02: 8780074 .. 8783002 (+)
RNA-Seq Expression	CmaCh02G015470
Synteny	CmaCh02G015470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACATACCATTCCCATACTTTTATTTCTTCACCTCAAAACCCTCACCATGAGCGTCCTCCCCCAAATGGATCAAGATAAAACTGTCCCAGATGCATGGGACTACACCGGCAAGCCCGCCATCAAAGCCAAAACCGGTGGCTGGGCAGCCGCTGCCATGATTCTAGGGCCGGAGGCAGTGGAGAGGATGACGACGCTGGGGATCGCAGTGAACTTGGTGACATATATGACCGACACAATGCATCTGGGCAATGCTGCCGCCGCCAACGATGTCACCAACTTCCTCGGAACCTCCTTCATGCTCTGTCTCTTCGGTGGTTTTGTCGCCGACACTTTTCTCGGCCGGTAATGGATGTTTCTTAAATTTGATTTAATACATCCAAAAAAATTTAATTTTTTCTACCATATTTTTGTGCTTATTCATTCAACATTTATAAAATTTTAATAAATTTTATTTTATTTTGTTTATTTAATTCTAATGGGTTTGATCTATATTCTTATAAAAGGTATTTGACGATTTCGATTTTCGCAACTGTTCAAGCAATGGTAAGATTATATCGATCATTAATTACTTTATTATTATCTTGAGAATTATTTAATTAATTAGTGGTAAATTAAATAAGCTTAAATCAAAATTTTATTGCATTAGGGTGTAACTCTCTTGACAATTTCCACCATAATTCCAAGTCTCCGACCACCAAAATGTTCGCCGGCGTTATCAACCACCCCGTGCATTCCAGCTGACAGCAAGCAGCTAGCAATTCTCCACGCAGCATTATACTTAACGGCGCTCGGCACCGGTGGCCTAAAATCGAGCGTATCGGGGTTCGGTTCCGATCAATTTATCGAAACAGACAAAGAGGAGAGAATAAAAATGTCCAACTTCTTCAACTGGTTCTTCTTCTTGATAAGCCTTGGGTCGCTTGCAGCTGTGACAGTCCTTGTTTACATTCAAGACAACTGGGGCAGGCAATGGGGATATGGGATATGTGCTTGTGCCATTGTGTTAGGATTGGCTATCTTCCTGATGGGGACTCGACGCTACCGGTTCAAGAAATTGGCCGGTAGCCCGTTGACACAGATCGCCGCTGTGTTCGTCACTGCATGGAAGAAGAGGAAGTTGGATTTGCCATCGGATTCGTCGTCGTTGTTTGAAATCGAAGACGCTGTGGATGGATCTGGGAAGAAGAAGCAGAAACTTCCTCGCTCCAAGCAATTCAGGTTGGTTTTTCTACGTATTTTAACCATTGAATAAAGTTTAATGCTACAATCAATATTAGTAATATAGTCAATATAGTTGGTATAATATGATAAACATATTCAAATCATATTACTTCTAAACATTATGGAAGTGACAATTATTACATAGTTTAGTCCATTTCCAATACATTTTCCAACCATATATTACCCAAAATAATTCAAGCAGACAAGCTTATGACAAAAAATATTTTCTATAATAATAATATTATTATATTATTAAATTGATTGAGTCTGCAAGTTGTGAAAAAGAATCAAAATTAAGAAATGAAATGGAATTGGGAGTCTCACACGCTGCTTGTCTCTTTTCCAAGAACAAACACAAATTTCTGCTTTGAATTTCCCATTTTTAATTCAAATTCTTATTTTTGTCTCATGGGTTTGAAAAAAGTTTCAAACTTTGATCCAACCCAAGTTCCAATTTGAGCTAAATTTCCAAGATAATCGGATGTTTTGTTTTTTGGGGCAGGTTTCTGGATAAGGCGGCGATCAAGGACGAAGAAAATGTTGGCAATGTAGTGAACAAGTGGACGTTATCAACCCTAACAGACATTGAGGAAGTTAAATTAGTTCTTCGAATGTTACCAATTTGGGCAACCACCATTATTTTCTGGACAGTCTATGCCCAAATGACCACGTTTTCAGTGTCACAAGCCACCACCATGAACCGACACATCGGAAAATCCTTCGAGATTCCAGCAGCCTCCCTCACTGCCTTCTTCGTTGCCAGCATCCTCTTAACAGTCCCGATCTACGACCGATTCATCGTCCCAGTAGCGTCGAGGGTTTTGAAGAACCCACAAGGTCTCTCTCCGCTGCAGCGTGTCGGGGTCGGCTTGGTTCTATCCATAATTGCCATGATCGCTGCCGCGCTGACGGAGATAAAGCGACTGAGGGTGGTGGAGGCACATGGATTGGTGGACAAGCCGCTGGCGGAGGTGCCATTGAGTGTGTTTTGGTTGTCGCCACAATTTTTAATAGTGGGATCAGGTGAGTGAAGAAGGGAGGAATTGGCAGCCCTTGCTTAAAGTTTTTAGCTTTGATGTTAAAAGGTTAAATTTTGTTGATGTTTGTAGGTGAGGCATTCACGTACATGGGGCAATTGGATTTCTTCCTAAGGGAGTGTCCTAAAGGAATGAAGACAATGAGCACAGGGCTGTTCTTGAGCACACTTTCTTTAGGGTTTTTCTTTAGCTCATTGCTGGTTACAATTGTGGGAAAAGTGACAGAAAATGGGAAGCCATGGATTCCAGACAACCTGAATGCAGGGAAGCTCTATGATTTCTATTGGCTATTGGCAATTTTGAGTGTGTTGAATTTGGTGGTGTTTTTGTTTTGTGCAAAGTGGTATGTGTACAAGGACAAGAGGTTGGCTGAGCAGGGGGTTGAATTGGAGGACTATGGGCCATCTTCCCATGCATAGCCATGCATGTATATATTATCACTTTCTCTTTGTTTATGTGTGGGCTTTTGTTAAGGACAAAAGCTACGAAGGCCTTTGTTTATGGGGTACCCTTTGCTTTCTAATAATGTAAAGTTCGTAAGGTTTTTGATGTACCAAAAGGCCAAGGAAAAGTGAGAATCCGTTTTGAAGTTCTCTAATATAATATCAACAGTTTGGGTGTTCCATTTTTTTTTTCTTTCAAACTCTCTAATATAATATCTGAGTGGGG

mRNA sequence

CCACATACCATTCCCATACTTTTATTTCTTCACCTCAAAACCCTCACCATGAGCGTCCTCCCCCAAATGGATCAAGATAAAACTGTCCCAGATGCATGGGACTACACCGGCAAGCCCGCCATCAAAGCCAAAACCGGTGGCTGGGCAGCCGCTGCCATGATTCTAGGGCCGGAGGCAGTGGAGAGGATGACGACGCTGGGGATCGCAGTGAACTTGGTGACATATATGACCGACACAATGCATCTGGGCAATGCTGCCGCCGCCAACGATGTCACCAACTTCCTCGGAACCTCCTTCATGCTCTGTCTCTTCGGTGGTTTTGTCGCCGACACTTTTCTCGGCCGGTATTTGACGATTTCGATTTTCGCAACTGTTCAAGCAATGGGTGTAACTCTCTTGACAATTTCCACCATAATTCCAAGTCTCCGACCACCAAAATGTTCGCCGGCGTTATCAACCACCCCGTGCATTCCAGCTGACAGCAAGCAGCTAGCAATTCTCCACGCAGCATTATACTTAACGGCGCTCGGCACCGGTGGCCTAAAATCGAGCGTATCGGGGTTCGGTTCCGATCAATTTATCGAAACAGACAAAGAGGAGAGAATAAAAATGTCCAACTTCTTCAACTGGTTCTTCTTCTTGATAAGCCTTGGGTCGCTTGCAGCTGTGACAGTCCTTGTTTACATTCAAGACAACTGGGGCAGGCAATGGGGATATGGGATATGTGCTTGTGCCATTGTGTTAGGATTGGCTATCTTCCTGATGGGGACTCGACGCTACCGGTTCAAGAAATTGGCCGGTAGCCCGTTGACACAGATCGCCGCTGTGTTCGTCACTGCATGGAAGAAGAGGAAGTTGGATTTGCCATCGGATTCGTCGTCGTTGTTTGAAATCGAAGACGCTGTGGATGGATCTGGGAAGAAGAAGCAGAAACTTCCTCGCTCCAAGCAATTCAGGTTTCTGGATAAGGCGGCGATCAAGGACGAAGAAAATGTTGGCAATGTAGTGAACAAGTGGACGTTATCAACCCTAACAGACATTGAGGAAGTTAAATTAGTTCTTCGAATGTTACCAATTTGGGCAACCACCATTATTTTCTGGACAGTCTATGCCCAAATGACCACGTTTTCAGTGTCACAAGCCACCACCATGAACCGACACATCGGAAAATCCTTCGAGATTCCAGCAGCCTCCCTCACTGCCTTCTTCGTTGCCAGCATCCTCTTAACAGTCCCGATCTACGACCGATTCATCGTCCCAGTAGCGTCGAGGGTTTTGAAGAACCCACAAGGTCTCTCTCCGCTGCAGCGTGTCGGGGTCGGCTTGGTTCTATCCATAATTGCCATGATCGCTGCCGCGCTGACGGAGATAAAGCGACTGAGGGTGGTGGAGGCACATGGATTGGTGGACAAGCCGCTGGCGGAGGTGCCATTGAGTGTGTTTTGGTTGTCGCCACAATTTTTAATAGTGGGATCAGGTGAGGCATTCACGTACATGGGGCAATTGGATTTCTTCCTAAGGGAGTGTCCTAAAGGAATGAAGACAATGAGCACAGGGCTGTTCTTGAGCACACTTTCTTTAGGGTTTTTCTTTAGCTCATTGCTGGTTACAATTGTGGGAAAAGTGACAGAAAATGGGAAGCCATGGATTCCAGACAACCTGAATGCAGGGAAGCTCTATGATTTCTATTGGCTATTGGCAATTTTGAGTGTGTTGAATTTGGTGGTGTTTTTGTTTTGTGCAAAGTGGTATGTGTACAAGGACAAGAGGTTGGCTGAGCAGGGGGTTGAATTGGAGGACTATGGGCCATCTTCCCATGCATAGCCATGCATGTATATATTATCACTTTCTCTTTGTTTATGTGTGGGCTTTTGTTAAGGACAAAAGCTACGAAGGCCTTTGTTTATGGGGTACCCTTTGCTTTCTAATAATGTAAAGTTCGTAAGGTTTTTGATGTACCAAAAGGCCAAGGAAAAGTGAGAATCCGTTTTGAAGTTCTCTAATATAATATCAACAGTTTGGGTGTTCCATTTTTTTTTTCTTTCAAACTCTCTAATATAATATCTGAGTGGGG

Coding sequence (CDS)

ATGAGCGTCCTCCCCCAAATGGATCAAGATAAAACTGTCCCAGATGCATGGGACTACACCGGCAAGCCCGCCATCAAAGCCAAAACCGGTGGCTGGGCAGCCGCTGCCATGATTCTAGGGCCGGAGGCAGTGGAGAGGATGACGACGCTGGGGATCGCAGTGAACTTGGTGACATATATGACCGACACAATGCATCTGGGCAATGCTGCCGCCGCCAACGATGTCACCAACTTCCTCGGAACCTCCTTCATGCTCTGTCTCTTCGGTGGTTTTGTCGCCGACACTTTTCTCGGCCGGTATTTGACGATTTCGATTTTCGCAACTGTTCAAGCAATGGGTGTAACTCTCTTGACAATTTCCACCATAATTCCAAGTCTCCGACCACCAAAATGTTCGCCGGCGTTATCAACCACCCCGTGCATTCCAGCTGACAGCAAGCAGCTAGCAATTCTCCACGCAGCATTATACTTAACGGCGCTCGGCACCGGTGGCCTAAAATCGAGCGTATCGGGGTTCGGTTCCGATCAATTTATCGAAACAGACAAAGAGGAGAGAATAAAAATGTCCAACTTCTTCAACTGGTTCTTCTTCTTGATAAGCCTTGGGTCGCTTGCAGCTGTGACAGTCCTTGTTTACATTCAAGACAACTGGGGCAGGCAATGGGGATATGGGATATGTGCTTGTGCCATTGTGTTAGGATTGGCTATCTTCCTGATGGGGACTCGACGCTACCGGTTCAAGAAATTGGCCGGTAGCCCGTTGACACAGATCGCCGCTGTGTTCGTCACTGCATGGAAGAAGAGGAAGTTGGATTTGCCATCGGATTCGTCGTCGTTGTTTGAAATCGAAGACGCTGTGGATGGATCTGGGAAGAAGAAGCAGAAACTTCCTCGCTCCAAGCAATTCAGGTTTCTGGATAAGGCGGCGATCAAGGACGAAGAAAATGTTGGCAATGTAGTGAACAAGTGGACGTTATCAACCCTAACAGACATTGAGGAAGTTAAATTAGTTCTTCGAATGTTACCAATTTGGGCAACCACCATTATTTTCTGGACAGTCTATGCCCAAATGACCACGTTTTCAGTGTCACAAGCCACCACCATGAACCGACACATCGGAAAATCCTTCGAGATTCCAGCAGCCTCCCTCACTGCCTTCTTCGTTGCCAGCATCCTCTTAACAGTCCCGATCTACGACCGATTCATCGTCCCAGTAGCGTCGAGGGTTTTGAAGAACCCACAAGGTCTCTCTCCGCTGCAGCGTGTCGGGGTCGGCTTGGTTCTATCCATAATTGCCATGATCGCTGCCGCGCTGACGGAGATAAAGCGACTGAGGGTGGTGGAGGCACATGGATTGGTGGACAAGCCGCTGGCGGAGGTGCCATTGAGTGTGTTTTGGTTGTCGCCACAATTTTTAATAGTGGGATCAGGTGAGGCATTCACGTACATGGGGCAATTGGATTTCTTCCTAAGGGAGTGTCCTAAAGGAATGAAGACAATGAGCACAGGGCTGTTCTTGAGCACACTTTCTTTAGGGTTTTTCTTTAGCTCATTGCTGGTTACAATTGTGGGAAAAGTGACAGAAAATGGGAAGCCATGGATTCCAGACAACCTGAATGCAGGGAAGCTCTATGATTTCTATTGGCTATTGGCAATTTTGAGTGTGTTGAATTTGGTGGTGTTTTTGTTTTGTGCAAAGTGGTATGTGTACAAGGACAAGAGGTTGGCTGAGCAGGGGGTTGAATTGGAGGACTATGGGCCATCTTCCCATGCATAG

Protein sequence

MSVLPQMDQDKTVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGNAAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRPPKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKMSNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKKLAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKAAIKDEENVGNVVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVGLVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKPWIPDNLNAGKLYDFYWLLAILSVLNLVVFLFCAKWYVYKDKRLAEQGVELEDYGPSSHA

Homology

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BLAST of CmaCh02G015470 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 1.5e-210
Identity = 378/585 (64.62%), Postives = 461/585 (78.80%), Query Frame = 0


Query: 4   LPQMDQDKTVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDT 63
           LP+   D  + DAWD+ G+PA ++KTGGWA+AAMIL  EAVER+TTLGI VNLVTY+T T
Sbjct: 3   LPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGT 62

Query: 64  MHLGNAAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTII 123
           MHLGNA AAN VTNFLGTSFMLCL GGF+ADTFLGRYLTI+IFA +QA GV++LT+STII
Sbjct: 63  MHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTII 122

Query: 124 PSLRPPKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKE 183
           P LRPP+C+P  S + C  A   QL +L+ ALYLTALGTGG+K+SVSGFGSDQF ET+ +
Sbjct: 123 PGLRPPRCNPTTS-SHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPK 182

Query: 184 ERIKMSNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRR 243
           ER KM+ FFN FFF I++GSL AVTVLVY+QD+ GR+WGYGICA AIVL L++FL GT R
Sbjct: 183 ERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNR 242

Query: 244 YRFKKLAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIED--AVDGSGKKKQKLPRSKQ 303
           YRFKKL GSP+TQ+AAV V AW+ RKL+LP+D S L++++D  A +GS K KQKLP ++Q
Sbjct: 243 YRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQ 302

Query: 304 FRFLDKAAIKDEEN--VGNVVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTT 363
           FR LDKAAI+D+E     NV NKWTLSTLTD+EEVK ++RMLPIWAT I+FWTV+AQ+TT
Sbjct: 303 FRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTT 362

Query: 364 FSVSQATTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPL 423
            SV+Q+ T++R IG SFEIP AS+  F+V  +LLT  +YDR  + +  ++   P GL PL
Sbjct: 363 LSVAQSETLDRSIG-SFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPL 422

Query: 424 QRVGVGLVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEA 483
           QR+G+GL    +AM  AAL E+KRLR   AHG   K L   PL  + L PQ+LIVG GEA
Sbjct: 423 QRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTL---PLGFYLLIPQYLIVGIGEA 482

Query: 484 FTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKPWIPDNLN 543
             Y GQLDFFLRECPKGMK MSTGL LSTL+LGFFFSS+LVTIV K T    PWI D+LN
Sbjct: 483 LIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLN 542

Query: 544 AGKLYDFYWLLAILSVLNLVVFLFCAKWYVYKDKRLAEQGVELED 585
            G+LY+FYWL+A+L  LN ++FL  +KWYVYK+KRLAE G+EL+D
Sbjct: 543 KGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDD 582

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BLAST of CmaCh02G015470 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 8.3e-164
Identity = 314/565 (55.58%), Postives = 404/565 (71.50%), Query Frame = 0


Query: 12  TVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGNAAA 71
           TV DA DY G+PA K+KTGGW  AA+ILG E VER++T+GIAVNLVTY+ +TMHL ++ +
Sbjct: 8   TVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTS 67

Query: 72  ANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRPPKC 131
           AN VT+F+GTSF+LCL GGF+AD+FLGR+ TI IF+T+QA+G   L ++T +P LRPP C
Sbjct: 68  ANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC 127

Query: 132 SPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKMSNF 191
                   CIPA + Q+ IL+ +LYL ALGTGGLKSS+SGFGSDQF + D +E+  M+ F
Sbjct: 128 HHG---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFF 187

Query: 192 FNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKKLAG 251
           FN FFF IS+G+L AVTVLVY+QD  GR W YGIC  ++ + + IFL GT+RYR+KK  G
Sbjct: 188 FNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQG 247

Query: 252 SPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKAAIK 311
           SP+ QI  V   A++KRK++LP   S ++  ED  +G      ++  + QF  LDKAAI 
Sbjct: 248 SPVVQIFQVIAAAFRKRKMELP--QSIVYLYEDNPEGI-----RIEHTDQFHLLDKAAIV 307

Query: 312 DEENVGN------VVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQA 371
            E +         + N W LS++T +EEVK+++R+LPIWATTIIFWT YAQM TFSV QA
Sbjct: 308 AEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQA 367

Query: 372 TTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVG 431
           +TM R+IG SF+IPA SLT FFVA+IL+T+ +YDR I+P   +  K   G S LQR+ +G
Sbjct: 368 STMRRNIG-SFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIG 427

Query: 432 LVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQ 491
           LVLS   M AAAL E KRL V ++          +P+SVF L PQF +VG+GEAF Y GQ
Sbjct: 428 LVLSTAGMAAAALVEQKRLSVAKS-----SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQ 487

Query: 492 LDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKP--WIPDNLNAGKL 551
           LDFF+ + PKGMKTMSTGLFL+TLSLGFF SS LV+IV +VT       W+ DN+N G+L
Sbjct: 488 LDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRL 547

Query: 552 YDFYWLLAILSVLNLVVFLFCAKWY 569
             FYWLL ILS +N VV++ CA W+
Sbjct: 548 DYFYWLLVILSGINFVVYIICALWF 555

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BLAST of CmaCh02G015470 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.3e-140
Identity = 281/563 (49.91%), Postives = 376/563 (66.79%), Query Frame = 0


Query: 15  DAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGNAAAAND 74
           +A+DY G P  K+KTGGW  A +ILG E  ER+  +GI++NLVTY+   +H+ +A +A  
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 75  VTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRPPKCSPA 134
           VTNF+GT  +L L GGF+AD  LGRY  ++I A+V A+GV LLT++T I S+RPP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 135 LST-TPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKMSNFFN 194
                 CI A+  QLA+L+ ALY  ALG GG+KS+VSGFGSDQF  +D +E  +M  FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 195 WFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKKLAGSP 254
            F+F IS+GSL AV  LVY+QDN GR WGYGI A  +V+   + L GT+RYRFKK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 255 LTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKAAIKDE 314
            T I  V   AWKKRK   P+  S L         +G     +P ++  + LDKAAI   
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL---------NGYDNTTVPHTEMLKCLDKAAISKN 315

Query: 315 ENVGN-----VVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTM 374
           E+  +       + W +ST+T +EEVKLV++++PIWAT I+FWT+Y+QMTTF+V QAT M
Sbjct: 316 ESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFM 375

Query: 375 NRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVGLVL 434
           +R +G SF +PA S +AF + +ILL   + +R  VP+  R+ K PQG++ LQR+GVGLV 
Sbjct: 376 DRKLG-SFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVF 435

Query: 435 SIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQLDF 494
           S+ AM  AA+ E  R    EA    DK      +S FWL PQ+ +VG+GEAF Y+GQL+F
Sbjct: 436 SMAAMAVAAVIENARR---EAAVNNDK-----KISAFWLVPQYFLVGAGEAFAYVGQLEF 495

Query: 495 FLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKPWIPDNLNAGKLYDFYW 554
           F+RE P+ MK+MSTGLFLST+S+GFF SSLLV++V +VT+  K W+  NLN  +L  FYW
Sbjct: 496 FIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTD--KSWLRSNLNKARLNYFYW 555

Query: 555 LLAILSVLNLVVFLFCAKWYVYK 572
           LL +L  LN ++F+  A  + YK
Sbjct: 556 LLVVLGALNFLIFIVFAMKHQYK 558

[Close]

BLAST of CmaCh02G015470 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 3.6e-135
Identity = 260/567 (45.86%), Postives = 376/567 (66.31%), Query Frame = 0


Query: 9   QDKTVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGN 68
           QD TV    D    PA K KTG W A   ILG E  ER+   G+  NLV Y+   ++ GN
Sbjct: 9   QDGTV----DIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 68

Query: 69  AAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRP 128
           A AAN+VTN+ GT ++  L G F+AD +LGRY TI+ F  +   G+TLLT+S  +P L+P
Sbjct: 69  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 128

Query: 129 PKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKM 188
             C    +   C P +S Q A+   ALY+ ALGTGG+K  VS FG+DQF E D+ E+IK 
Sbjct: 129 GNC----NADTCHP-NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 188

Query: 189 SNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKK 248
           S+FFNWF+F I++G+L A TVLV+IQ N G  WG+G+   A+V+ +  F  G+R YR ++
Sbjct: 189 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 248

Query: 249 LAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKA 308
             GSPLT+I  V V A++K  + +P D S LFE  D  + + K  +KL  +   +F DKA
Sbjct: 249 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADD-ESNIKGSRKLVHTDNLKFFDKA 308

Query: 309 AIKDEEN--VGNVVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQAT 368
           A++ + +      VN W L ++T +EE+K ++ +LP+WAT I+F TVY+QM+T  V Q  
Sbjct: 309 AVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGN 368

Query: 369 TMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVGL 428
           TM++H+GK+FEIP+ASL+ F   S+L   P+YD+FI+P+A +  +N +G + LQR+G+GL
Sbjct: 369 TMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGL 428

Query: 429 VLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQL 488
           V+SI AMI A + E+ RL  V+ H   D+   ++ +S+FW  PQ+L++G  E FT++GQL
Sbjct: 429 VVSIFAMITAGVLEVVRLDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQL 488

Query: 489 DFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVT-ENGKP-WIPDNLNAGKLY 548
           +FF  + P  M+++ + L L+T++LG + S++LVT+V K+T +NGKP WIPDNLN G L 
Sbjct: 489 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 548

Query: 549 DFYWLLAILSVLNLVVFLFCAKWYVYK 572
            F++LLA LS LN +V+L+ +K Y YK
Sbjct: 549 YFFYLLATLSFLNFLVYLWISKRYKYK 563

[Close]

BLAST of CmaCh02G015470 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.9e-124
Identity = 248/572 (43.36%), Postives = 366/572 (63.99%), Query Frame = 0


Query: 7   MDQDK---TVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDT 66
           M+ DK   T     D   KPA K KTG W A   ILG E  ER+   G++ NL+ Y+   
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 67  MHLGNAAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTII 126
           M++ N +A+  V+N+ GT +   L G F+AD +LGRY TI+ F  +   G+TLLTIS  +
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 127 PSLRPPKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKE 186
           P L     +P  S   C  A + Q AI   ALYL ALGTGG+K  VS FG+DQF +TD++
Sbjct: 121 PGL-----TPTCSGETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEK 180

Query: 187 ERIKMSNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRR 246
           E+   S+FFNWF+F+I++G++ A +VLV+IQ N G  WG G+   A+ + +  F  G+  
Sbjct: 181 EKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNF 240

Query: 247 YRFKKLAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFR 306
           YR +K  GSPLT++  V V + +K K+ +P D S L+E +DA + S    +KL  +K   
Sbjct: 241 YRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDA-ESSIIGSRKLEHTKILT 300

Query: 307 FLDKAAIKDE-ENVGNV-VNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 366
           F DKAA++ E +N G    + W L T+T +EE+K ++R+LPIWAT I+F +VY+QM T  
Sbjct: 301 FFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVF 360

Query: 367 VSQATTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQR 426
           V Q  T+++H+G +F+IP+ASL+ F   S+L   P+YD+ IVP A +   + +G + LQR
Sbjct: 361 VLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQR 420

Query: 427 VGVGLVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFT 486
           +G+GLV+SI +M++A + E+ RL  V+ H L ++    +P+++FW  PQ+ +VG  E FT
Sbjct: 421 IGIGLVISIFSMVSAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFT 480

Query: 487 YMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTEN-GKP-WIPDNLN 546
           ++GQL+FF  + P  M+++ + L L+ ++ G + S+ LVT+V KVT + G+P WI  NLN
Sbjct: 481 FIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLN 540

Query: 547 AGKLYDFYWLLAILSVLNLVVFLFCAKWYVYK 572
            G L  F+WLLA LS LN +V+L+ AKWY YK
Sbjct: 541 NGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

[Close]

BLAST of CmaCh02G015470 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 733.8 bits (1893), Expect = 1.1e-211
Identity = 378/585 (64.62%), Postives = 461/585 (78.80%), Query Frame = 0


Query: 4   LPQMDQDKTVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDT 63
           LP+   D  + DAWD+ G+PA ++KTGGWA+AAMIL  EAVER+TTLGI VNLVTY+T T
Sbjct: 3   LPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGT 62

Query: 64  MHLGNAAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTII 123
           MHLGNA AAN VTNFLGTSFMLCL GGF+ADTFLGRYLTI+IFA +QA GV++LT+STII
Sbjct: 63  MHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTII 122

Query: 124 PSLRPPKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKE 183
           P LRPP+C+P  S + C  A   QL +L+ ALYLTALGTGG+K+SVSGFGSDQF ET+ +
Sbjct: 123 PGLRPPRCNPTTS-SHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPK 182

Query: 184 ERIKMSNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRR 243
           ER KM+ FFN FFF I++GSL AVTVLVY+QD+ GR+WGYGICA AIVL L++FL GT R
Sbjct: 183 ERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNR 242

Query: 244 YRFKKLAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIED--AVDGSGKKKQKLPRSKQ 303
           YRFKKL GSP+TQ+AAV V AW+ RKL+LP+D S L++++D  A +GS K KQKLP ++Q
Sbjct: 243 YRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQ 302

Query: 304 FRFLDKAAIKDEEN--VGNVVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTT 363
           FR LDKAAI+D+E     NV NKWTLSTLTD+EEVK ++RMLPIWAT I+FWTV+AQ+TT
Sbjct: 303 FRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTT 362

Query: 364 FSVSQATTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPL 423
            SV+Q+ T++R IG SFEIP AS+  F+V  +LLT  +YDR  + +  ++   P GL PL
Sbjct: 363 LSVAQSETLDRSIG-SFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPL 422

Query: 424 QRVGVGLVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEA 483
           QR+G+GL    +AM  AAL E+KRLR   AHG   K L   PL  + L PQ+LIVG GEA
Sbjct: 423 QRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTL---PLGFYLLIPQYLIVGIGEA 482

Query: 484 FTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKPWIPDNLN 543
             Y GQLDFFLRECPKGMK MSTGL LSTL+LGFFFSS+LVTIV K T    PWI D+LN
Sbjct: 483 LIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLN 542

Query: 544 AGKLYDFYWLLAILSVLNLVVFLFCAKWYVYKDKRLAEQGVELED 585
            G+LY+FYWL+A+L  LN ++FL  +KWYVYK+KRLAE G+EL+D
Sbjct: 543 KGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDD 582

[Close]

BLAST of CmaCh02G015470 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 578.6 bits (1490), Expect = 5.9e-165
Identity = 314/565 (55.58%), Postives = 404/565 (71.50%), Query Frame = 0


Query: 12  TVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGNAAA 71
           TV DA DY G+PA K+KTGGW  AA+ILG E VER++T+GIAVNLVTY+ +TMHL ++ +
Sbjct: 8   TVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTS 67

Query: 72  ANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRPPKC 131
           AN VT+F+GTSF+LCL GGF+AD+FLGR+ TI IF+T+QA+G   L ++T +P LRPP C
Sbjct: 68  ANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC 127

Query: 132 SPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKMSNF 191
                   CIPA + Q+ IL+ +LYL ALGTGGLKSS+SGFGSDQF + D +E+  M+ F
Sbjct: 128 HHG---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFF 187

Query: 192 FNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKKLAG 251
           FN FFF IS+G+L AVTVLVY+QD  GR W YGIC  ++ + + IFL GT+RYR+KK  G
Sbjct: 188 FNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQG 247

Query: 252 SPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKAAIK 311
           SP+ QI  V   A++KRK++LP   S ++  ED  +G      ++  + QF  LDKAAI 
Sbjct: 248 SPVVQIFQVIAAAFRKRKMELP--QSIVYLYEDNPEGI-----RIEHTDQFHLLDKAAIV 307

Query: 312 DEENVGN------VVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQA 371
            E +         + N W LS++T +EEVK+++R+LPIWATTIIFWT YAQM TFSV QA
Sbjct: 308 AEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQA 367

Query: 372 TTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVG 431
           +TM R+IG SF+IPA SLT FFVA+IL+T+ +YDR I+P   +  K   G S LQR+ +G
Sbjct: 368 STMRRNIG-SFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIG 427

Query: 432 LVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQ 491
           LVLS   M AAAL E KRL V ++          +P+SVF L PQF +VG+GEAF Y GQ
Sbjct: 428 LVLSTAGMAAAALVEQKRLSVAKS-----SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQ 487

Query: 492 LDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKP--WIPDNLNAGKL 551
           LDFF+ + PKGMKTMSTGLFL+TLSLGFF SS LV+IV +VT       W+ DN+N G+L
Sbjct: 488 LDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRL 547

Query: 552 YDFYWLLAILSVLNLVVFLFCAKWY 569
             FYWLL ILS +N VV++ CA W+
Sbjct: 548 DYFYWLLVILSGINFVVYIICALWF 555

[Close]

BLAST of CmaCh02G015470 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 501.5 bits (1290), Expect = 9.1e-142
Identity = 281/563 (49.91%), Postives = 376/563 (66.79%), Query Frame = 0


Query: 15  DAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGNAAAAND 74
           +A+DY G P  K+KTGGW  A +ILG E  ER+  +GI++NLVTY+   +H+ +A +A  
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 75  VTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRPPKCSPA 134
           VTNF+GT  +L L GGF+AD  LGRY  ++I A+V A+GV LLT++T I S+RPP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 135 LST-TPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKMSNFFN 194
                 CI A+  QLA+L+ ALY  ALG GG+KS+VSGFGSDQF  +D +E  +M  FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 195 WFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKKLAGSP 254
            F+F IS+GSL AV  LVY+QDN GR WGYGI A  +V+   + L GT+RYRFKK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 255 LTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKAAIKDE 314
            T I  V   AWKKRK   P+  S L         +G     +P ++  + LDKAAI   
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL---------NGYDNTTVPHTEMLKCLDKAAISKN 315

Query: 315 ENVGN-----VVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQATTM 374
           E+  +       + W +ST+T +EEVKLV++++PIWAT I+FWT+Y+QMTTF+V QAT M
Sbjct: 316 ESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFM 375

Query: 375 NRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVGLVL 434
           +R +G SF +PA S +AF + +ILL   + +R  VP+  R+ K PQG++ LQR+GVGLV 
Sbjct: 376 DRKLG-SFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVF 435

Query: 435 SIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQLDF 494
           S+ AM  AA+ E  R    EA    DK      +S FWL PQ+ +VG+GEAF Y+GQL+F
Sbjct: 436 SMAAMAVAAVIENARR---EAAVNNDK-----KISAFWLVPQYFLVGAGEAFAYVGQLEF 495

Query: 495 FLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTENGKPWIPDNLNAGKLYDFYW 554
           F+RE P+ MK+MSTGLFLST+S+GFF SSLLV++V +VT+  K W+  NLN  +L  FYW
Sbjct: 496 FIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTD--KSWLRSNLNKARLNYFYW 555

Query: 555 LLAILSVLNLVVFLFCAKWYVYK 572
           LL +L  LN ++F+  A  + YK
Sbjct: 556 LLVVLGALNFLIFIVFAMKHQYK 558

[Close]

BLAST of CmaCh02G015470 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 483.4 bits (1243), Expect = 2.6e-136
Identity = 260/567 (45.86%), Postives = 376/567 (66.31%), Query Frame = 0


Query: 9   QDKTVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDTMHLGN 68
           QD TV    D    PA K KTG W A   ILG E  ER+   G+  NLV Y+   ++ GN
Sbjct: 9   QDGTV----DIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 68

Query: 69  AAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTIIPSLRP 128
           A AAN+VTN+ GT ++  L G F+AD +LGRY TI+ F  +   G+TLLT+S  +P L+P
Sbjct: 69  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 128

Query: 129 PKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKEERIKM 188
             C    +   C P +S Q A+   ALY+ ALGTGG+K  VS FG+DQF E D+ E+IK 
Sbjct: 129 GNC----NADTCHP-NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 188

Query: 189 SNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRRYRFKK 248
           S+FFNWF+F I++G+L A TVLV+IQ N G  WG+G+   A+V+ +  F  G+R YR ++
Sbjct: 189 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 248

Query: 249 LAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFRFLDKA 308
             GSPLT+I  V V A++K  + +P D S LFE  D  + + K  +KL  +   +F DKA
Sbjct: 249 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADD-ESNIKGSRKLVHTDNLKFFDKA 308

Query: 309 AIKDEEN--VGNVVNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFSVSQAT 368
           A++ + +      VN W L ++T +EE+K ++ +LP+WAT I+F TVY+QM+T  V Q  
Sbjct: 309 AVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGN 368

Query: 369 TMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQRVGVGL 428
           TM++H+GK+FEIP+ASL+ F   S+L   P+YD+FI+P+A +  +N +G + LQR+G+GL
Sbjct: 369 TMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGL 428

Query: 429 VLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFTYMGQL 488
           V+SI AMI A + E+ RL  V+ H   D+   ++ +S+FW  PQ+L++G  E FT++GQL
Sbjct: 429 VVSIFAMITAGVLEVVRLDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQL 488

Query: 489 DFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVT-ENGKP-WIPDNLNAGKLY 548
           +FF  + P  M+++ + L L+T++LG + S++LVT+V K+T +NGKP WIPDNLN G L 
Sbjct: 489 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 548

Query: 549 DFYWLLAILSVLNLVVFLFCAKWYVYK 572
            F++LLA LS LN +V+L+ +K Y YK
Sbjct: 549 YFFYLLATLSFLNFLVYLWISKRYKYK 563

[Close]

BLAST of CmaCh02G015470 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 447.2 bits (1149), Expect = 2.0e-125
Identity = 248/572 (43.36%), Postives = 366/572 (63.99%), Query Frame = 0


Query: 7   MDQDK---TVPDAWDYTGKPAIKAKTGGWAAAAMILGPEAVERMTTLGIAVNLVTYMTDT 66
           M+ DK   T     D   KPA K KTG W A   ILG E  ER+   G++ NL+ Y+   
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 67  MHLGNAAAANDVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAMGVTLLTISTII 126
           M++ N +A+  V+N+ GT +   L G F+AD +LGRY TI+ F  +   G+TLLTIS  +
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 127 PSLRPPKCSPALSTTPCIPADSKQLAILHAALYLTALGTGGLKSSVSGFGSDQFIETDKE 186
           P L     +P  S   C  A + Q AI   ALYL ALGTGG+K  VS FG+DQF +TD++
Sbjct: 121 PGL-----TPTCSGETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEK 180

Query: 187 ERIKMSNFFNWFFFLISLGSLAAVTVLVYIQDNWGRQWGYGICACAIVLGLAIFLMGTRR 246
           E+   S+FFNWF+F+I++G++ A +VLV+IQ N G  WG G+   A+ + +  F  G+  
Sbjct: 181 EKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNF 240

Query: 247 YRFKKLAGSPLTQIAAVFVTAWKKRKLDLPSDSSSLFEIEDAVDGSGKKKQKLPRSKQFR 306
           YR +K  GSPLT++  V V + +K K+ +P D S L+E +DA + S    +KL  +K   
Sbjct: 241 YRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDA-ESSIIGSRKLEHTKILT 300

Query: 307 FLDKAAIKDE-ENVGNV-VNKWTLSTLTDIEEVKLVLRMLPIWATTIIFWTVYAQMTTFS 366
           F DKAA++ E +N G    + W L T+T +EE+K ++R+LPIWAT I+F +VY+QM T  
Sbjct: 301 FFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVF 360

Query: 367 VSQATTMNRHIGKSFEIPAASLTAFFVASILLTVPIYDRFIVPVASRVLKNPQGLSPLQR 426
           V Q  T+++H+G +F+IP+ASL+ F   S+L   P+YD+ IVP A +   + +G + LQR
Sbjct: 361 VLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQR 420

Query: 427 VGVGLVLSIIAMIAAALTEIKRLRVVEAHGLVDKPLAEVPLSVFWLSPQFLIVGSGEAFT 486
           +G+GLV+SI +M++A + E+ RL  V+ H L ++    +P+++FW  PQ+ +VG  E FT
Sbjct: 421 IGIGLVISIFSMVSAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFT 480

Query: 487 YMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVGKVTEN-GKP-WIPDNLN 546
           ++GQL+FF  + P  M+++ + L L+ ++ G + S+ LVT+V KVT + G+P WI  NLN
Sbjct: 481 FIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLN 540

Query: 547 AGKLYDFYWLLAILSVLNLVVFLFCAKWYVYK 572
            G L  F+WLLA LS LN +V+L+ AKWY YK
Sbjct: 541 NGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

The following BLAST results are available for this feature:

BLAST of CmaCh02G015470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05085	1.5e-210	64.62	Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1	[more]
Q9SZY4	8.3e-164	55.58	Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1	[more]
Q9LVE0	1.3e-140	49.91	Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1	[more]
Q9M390	3.6e-135	45.86	Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1	[more]
Q9LFB8	2.9e-124	43.36	Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1	[more]

BLAST of CmaCh02G015470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12110.1	1.1e-211	64.62	nitrate transporter 1.1	[more]
AT2G26690.1	5.9e-165	55.58	Major facilitator superfamily protein	[more]
AT3G21670.1	9.1e-142	49.91	Major facilitator superfamily protein	[more]
AT3G54140.1	2.6e-136	45.86	peptide transporter 1	[more]
AT5G01180.1	2.0e-125	43.36	peptide transporter 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 3..578 e-value: 4.9E-199 score: 663.9
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 30..572
IPR000109	Proton-dependent oligopeptide transporter family	PFAM	PF00854	PTR2	coord: 100..534 e-value: 3.3E-115 score: 385.1
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 11..579
None	No IPR available	PANTHER	PTHR11654:SF507	PROTEIN NRT1/ PTR FAMILY 6.3	coord: 11..579
IPR018456	PTR2 family proton/oligopeptide symporter, conserved site	PROSITE	PS01022	PTR2_1	coord: 89..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G015470.1	CmaCh02G015470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015706	nitrate transport
biological_process	GO:0006857	oligopeptide transport
biological_process	GO:0010167	response to nitrate
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity
molecular_function	GO:0022857	transmembrane transporter activity