CmaCh02G015280 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh02G015280 vs. ExPASy Swiss-Prot
Match: Q9ZW85 (3-isopropylmalate dehydratase small subunit 1 OS=Arabidopsis thaliana OX=3702 GN=SSU1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.4e-84
Identity = 166/256 (64.84%), Postives = 195/256 (76.17%), Query Frame = 0

Query: 2   TMAASLYLSQSPIATATRRSSPFISLIPPSQSIKFPSLHCHTLKDLSITASHASQRAATI 61
           T++ +L     P + AT R SPF+     S +  F  L              + + +++ 
Sbjct: 11  TLSRTLASPNKPSSFATFR-SPFLRFNSTSVASNFKPL-------------VSREASSSF 70

Query: 62  IPRAAATPSSAASSTTVFHGSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIG 121
           + R+AA P    +    FHG CYVVGDNIDTDQIIPAE+LTLVPSNP EYEKLGSYAL+G
Sbjct: 71  VTRSAAEPQERKT----FHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVG 130

Query: 122 LPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFR 181
           LP+SY  RFV+P EMKTKYSIII G+NFGCGSSREHAPV LGA+GA AVVA+SYARIFFR
Sbjct: 131 LPASYKERFVQPGEMKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFR 190

Query: 182 NSVSTGEIYPLESEIRICDECKTGDVLTIELAE--SRLINHTTGKEYKLKPIGDAGPVIE 241
           NSV+TGE+YPL+SE+R+CDEC TGDV T+EL E  S LINHTTGKEYKLKPIGDAGPVI+
Sbjct: 191 NSVATGEVYPLDSEVRVCDECTTGDVATVELREGDSILINHTTGKEYKLKPIGDAGPVID 248

Query: 242 AGGIFAYARKSGMIPS 256
           AGGIFAYARK+GMIPS
Sbjct: 251 AGGIFAYARKAGMIPS 248

BLAST of CmaCh02G015280 vs. ExPASy Swiss-Prot
Match: Q9LYT7 (3-isopropylmalate dehydratase small subunit 3 OS=Arabidopsis thaliana OX=3702 GN=SSU3 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.3e-78
Identity = 151/203 (74.38%), Postives = 170/203 (83.74%), Query Frame = 0

Query: 58  AATIIPRAAATPSSAASSTT--VFHGSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLG 117
           +ATII R AA+ S +  S T   FHG C+V+ DNIDTDQIIPAEY TL+PS P + EKLG
Sbjct: 50  SATIITRVAASSSDSGESITRETFHGLCFVLKDNIDTDQIIPAEYGTLIPSIPEDREKLG 109

Query: 118 SYALIGLPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESY 177
           S+AL GLP  Y+ RFV P EMK+KYS+II GDNFGCGSSREHAPV LGA+GA AVVAESY
Sbjct: 110 SFALNGLPKFYNERFVVPGEMKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESY 169

Query: 178 ARIFFRNSVSTGEIYPLESEIRICDECKTGDVLTIELAE---SRLINHTTGKEYKLKPIG 237
           ARIFFRN V+TGEI+PLESE+RICDECKTGDV+TIE  E   S LINHTT KEYKLKP+G
Sbjct: 170 ARIFFRNCVATGEIFPLESEVRICDECKTGDVVTIEHKEDGSSLLINHTTRKEYKLKPLG 229

Query: 238 DAGPVIEAGGIFAYARKSGMIPS 256
           DAGPVI+AGGIFAYARK+GMIPS
Sbjct: 230 DAGPVIDAGGIFAYARKAGMIPS 252

BLAST of CmaCh02G015280 vs. ExPASy Swiss-Prot
Match: Q9ZW84 (3-isopropylmalate dehydratase small subunit 2 OS=Arabidopsis thaliana OX=3702 GN=SSU2 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 5.1e-73
Identity = 140/213 (65.73%), Postives = 169/213 (79.34%), Query Frame = 0

Query: 48  SITASHASQRAATIIPRAAATPSSA--ASSTTVFHGSCYVVGDNIDTDQIIPAEYLTLVP 107
           SI+ +     + TIIPRAAA+ S +  A + T FHG CYV+ DNIDTDQIIPA      P
Sbjct: 43  SISITSRGTSSPTIIPRAAASESDSNEALANTTFHGLCYVLKDNIDTDQIIPAGAACTFP 102

Query: 108 SNPSEYEKLGSYALIGLPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGAS 167
           SN  E +++ ++AL GLP  + TRF+EP E ++KYSIII G+NFGCGSSREHAPV LGA+
Sbjct: 103 SNQQERDEIAAHALSGLPDFHKTRFIEPGENRSKYSIIIGGENFGCGSSREHAPVCLGAA 162

Query: 168 GAVAVVAESYARIFFRNSVSTGEIYPLESEIRICDECKTGDVLTIELAES--RLINHTTG 227
           GA A+VAESYARIFFRNSV+TGE++PLESE+R+C+ECKTGD +TIEL++S   L NHTTG
Sbjct: 163 GAKAIVAESYARIFFRNSVATGEVFPLESEVRVCEECKTGDTVTIELSDSGGLLTNHTTG 222

Query: 228 KEYKLKPIGDAGPVIEAGGIFAYARKSGMIPSL 257
           K YKLK IGDAGPVI+AGGIFAYAR  GMIPSL
Sbjct: 223 KNYKLKSIGDAGPVIDAGGIFAYARMMGMIPSL 255

BLAST of CmaCh02G015280 vs. ExPASy Swiss-Prot
Match: B5YHE5 (3-isopropylmalate dehydratase small subunit OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) OX=289376 GN=leuD PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.0e-28
Identity = 75/170 (44.12%), Postives = 105/170 (61.76%), Query Frame = 0

Query: 81  GSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIGLPSSYSTRFVEPNEMKTKY 140
           G  +  GDNIDTD IIPA YL     N S+ ++L  + +    SS+ ++ V+P +     
Sbjct: 5   GKVWKFGDNIDTDAIIPARYL-----NTSDPKELAKHVMEDADSSFPSK-VKPGD----- 64

Query: 141 SIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVSTGEIYPLESEIRICD 200
            IIIAG NFGCGSSREHAP+A+ ASG  AV+A+SYARIFFRN+ + G   P+     + D
Sbjct: 65  -IIIAGRNFGCGSSREHAPIAIKASGIRAVIAKSYARIFFRNAFNIG--LPIFEVPELID 124

Query: 201 ECKTGDVLTIELAESRLINHTTGKEYKLKPIGD-AGPVIEAGGIFAYARK 250
           E   GD + I++   ++IN T GK+Y  KPI      +I+AGG+  + +K
Sbjct: 125 ETNEGDEVEIDMNSGQIINLTKGKKYNTKPIPPFMQELIKAGGLVEWTKK 160

BLAST of CmaCh02G015280 vs. ExPASy Swiss-Prot
Match: Q9WZ25 (3-isopropylmalate dehydratase small subunit 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=leuD2 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.5e-27
Identity = 71/170 (41.76%), Postives = 104/170 (61.18%), Query Frame = 0

Query: 81  GSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIGLPSSYSTRFVEPNEMKTKY 140
           G  +V GDN++TD+IIPA YL     N S+ ++L  Y +      +  R         K 
Sbjct: 7   GKVFVFGDNVNTDEIIPARYL-----NTSDPQELAKYCMEDARPGFGRR------DDIKG 66

Query: 141 SIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVSTGEIYPLESEIRICD 200
           SII+AG+NFGCGSSREHAPVA+ A+G   V+A+S+ARIFFRN+++ G   P+  E++  D
Sbjct: 67  SIIVAGENFGCGSSREHAPVAIKAAGISCVIAKSFARIFFRNAINIG--LPI-VELKEAD 126

Query: 201 ECKTGDVLTIELAESRLINHTTGKEYKLKPIGD-AGPVIEAGGIFAYARK 250
           E + GD+  ++L    + N T GKEY+++P  +    ++EAGG   Y  K
Sbjct: 127 EFEEGDIAEVDLENGVVRNLTKGKEYRIRPYPEFLMKIMEAGGWLEYCLK 162

BLAST of CmaCh02G015280 vs. TAIR 10
Match: AT2G43090.1 (Aconitase/3-isopropylmalate dehydratase protein )

HSP 1 Score: 312.0 bits (798), Expect = 4.5e-85
Identity = 166/256 (64.84%), Postives = 195/256 (76.17%), Query Frame = 0

Query: 2   TMAASLYLSQSPIATATRRSSPFISLIPPSQSIKFPSLHCHTLKDLSITASHASQRAATI 61
           T++ +L     P + AT R SPF+     S +  F  L              + + +++ 
Sbjct: 11  TLSRTLASPNKPSSFATFR-SPFLRFNSTSVASNFKPL-------------VSREASSSF 70

Query: 62  IPRAAATPSSAASSTTVFHGSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIG 121
           + R+AA P    +    FHG CYVVGDNIDTDQIIPAE+LTLVPSNP EYEKLGSYAL+G
Sbjct: 71  VTRSAAEPQERKT----FHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVG 130

Query: 122 LPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFR 181
           LP+SY  RFV+P EMKTKYSIII G+NFGCGSSREHAPV LGA+GA AVVA+SYARIFFR
Sbjct: 131 LPASYKERFVQPGEMKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFR 190

Query: 182 NSVSTGEIYPLESEIRICDECKTGDVLTIELAE--SRLINHTTGKEYKLKPIGDAGPVIE 241
           NSV+TGE+YPL+SE+R+CDEC TGDV T+EL E  S LINHTTGKEYKLKPIGDAGPVI+
Sbjct: 191 NSVATGEVYPLDSEVRVCDECTTGDVATVELREGDSILINHTTGKEYKLKPIGDAGPVID 248

Query: 242 AGGIFAYARKSGMIPS 256
           AGGIFAYARK+GMIPS
Sbjct: 251 AGGIFAYARKAGMIPS 248

BLAST of CmaCh02G015280 vs. TAIR 10
Match: AT3G58990.1 (isopropylmalate isomerase 1 )

HSP 1 Score: 293.5 bits (750), Expect = 1.7e-79
Identity = 151/203 (74.38%), Postives = 170/203 (83.74%), Query Frame = 0

Query: 58  AATIIPRAAATPSSAASSTT--VFHGSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLG 117
           +ATII R AA+ S +  S T   FHG C+V+ DNIDTDQIIPAEY TL+PS P + EKLG
Sbjct: 50  SATIITRVAASSSDSGESITRETFHGLCFVLKDNIDTDQIIPAEYGTLIPSIPEDREKLG 109

Query: 118 SYALIGLPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESY 177
           S+AL GLP  Y+ RFV P EMK+KYS+II GDNFGCGSSREHAPV LGA+GA AVVAESY
Sbjct: 110 SFALNGLPKFYNERFVVPGEMKSKYSVIIGGDNFGCGSSREHAPVCLGAAGAKAVVAESY 169

Query: 178 ARIFFRNSVSTGEIYPLESEIRICDECKTGDVLTIELAE---SRLINHTTGKEYKLKPIG 237
           ARIFFRN V+TGEI+PLESE+RICDECKTGDV+TIE  E   S LINHTT KEYKLKP+G
Sbjct: 170 ARIFFRNCVATGEIFPLESEVRICDECKTGDVVTIEHKEDGSSLLINHTTRKEYKLKPLG 229

Query: 238 DAGPVIEAGGIFAYARKSGMIPS 256
           DAGPVI+AGGIFAYARK+GMIPS
Sbjct: 230 DAGPVIDAGGIFAYARKAGMIPS 252

BLAST of CmaCh02G015280 vs. TAIR 10
Match: AT2G43100.1 (isopropylmalate isomerase 2 )

HSP 1 Score: 275.8 bits (704), Expect = 3.6e-74
Identity = 140/213 (65.73%), Postives = 169/213 (79.34%), Query Frame = 0

Query: 48  SITASHASQRAATIIPRAAATPSSA--ASSTTVFHGSCYVVGDNIDTDQIIPAEYLTLVP 107
           SI+ +     + TIIPRAAA+ S +  A + T FHG CYV+ DNIDTDQIIPA      P
Sbjct: 43  SISITSRGTSSPTIIPRAAASESDSNEALANTTFHGLCYVLKDNIDTDQIIPAGAACTFP 102

Query: 108 SNPSEYEKLGSYALIGLPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGAS 167
           SN  E +++ ++AL GLP  + TRF+EP E ++KYSIII G+NFGCGSSREHAPV LGA+
Sbjct: 103 SNQQERDEIAAHALSGLPDFHKTRFIEPGENRSKYSIIIGGENFGCGSSREHAPVCLGAA 162

Query: 168 GAVAVVAESYARIFFRNSVSTGEIYPLESEIRICDECKTGDVLTIELAES--RLINHTTG 227
           GA A+VAESYARIFFRNSV+TGE++PLESE+R+C+ECKTGD +TIEL++S   L NHTTG
Sbjct: 163 GAKAIVAESYARIFFRNSVATGEVFPLESEVRVCEECKTGDTVTIELSDSGGLLTNHTTG 222

Query: 228 KEYKLKPIGDAGPVIEAGGIFAYARKSGMIPSL 257
           K YKLK IGDAGPVI+AGGIFAYAR  GMIPSL
Sbjct: 223 KNYKLKSIGDAGPVIDAGGIFAYARMMGMIPSL 255

BLAST of CmaCh02G015280 vs. TAIR 10
Match: AT2G43090.2 (Aconitase/3-isopropylmalate dehydratase protein )

HSP 1 Score: 244.6 bits (623), Expect = 8.9e-65
Identity = 141/256 (55.08%), Postives = 169/256 (66.02%), Query Frame = 0

Query: 2   TMAASLYLSQSPIATATRRSSPFISLIPPSQSIKFPSLHCHTLKDLSITASHASQRAATI 61
           T++ +L     P + AT R SPF+     S +  F  L              + + +++ 
Sbjct: 11  TLSRTLASPNKPSSFATFR-SPFLRFNSTSVASNFKPL-------------VSREASSSF 70

Query: 62  IPRAAATPSSAASSTTVFHGSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIG 121
           + R+AA P    +    FHG CYVVGDNIDTDQIIPAE+LTLVPSNP EYEKLGSYAL+G
Sbjct: 71  VTRSAAEPQERKT----FHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVG 130

Query: 122 LPSSYSTRFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFR 181
           LP+SY  RFV+P                             GA+GA AVVA+SYARIFFR
Sbjct: 131 LPASYKERFVQP-----------------------------GAAGAKAVVAQSYARIFFR 190

Query: 182 NSVSTGEIYPLESEIRICDECKTGDVLTIELAE--SRLINHTTGKEYKLKPIGDAGPVIE 241
           NSV+TGE+YPL+SE+R+CDEC TGDV T+EL E  S LINHTTGKEYKLKPIGDAGPVI+
Sbjct: 191 NSVATGEVYPLDSEVRVCDECTTGDVATVELREGDSILINHTTGKEYKLKPIGDAGPVID 219

Query: 242 AGGIFAYARKSGMIPS 256
           AGGIFAYARK+GMIPS
Sbjct: 251 AGGIFAYARKAGMIPS 219