CmaCh02G014540 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G014540
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Gamma-ECS
Location	Cma_Chr02: 8361167 .. 8366610 (+)
RNA-Seq Expression	CmaCh02G014540
Synteny	CmaCh02G014540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAATTTCTTACAAAAAGGAAAAGCGAATTCGAATTCAGCGCCGGAGGGTTCTACGCTTCCAGGTAATTGTTTGATGATGAAATTCCGAAATCAACTCCATGTCCGATCCATATTTATGTTCTGCAGTTGATTTTTCTTTCAGTTTTCGACCTTGTTGTTGTAGTATGGATTTTTCTTTACTGTATTTTGCTGTTTTTTTATGGTTTTCTTCGGAATAGAGTGTAACTGAGCGGTTGCCTACTTTTTAGAATCGCGCAATGGCTCTGGTTTCACAGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGATGGAGTGGAGGATTTATGGCTTTCAAGCTGTGCACTCATATGGAGTCGTTGAAGAGGAAGGAGAATCTTATTAGTGCGTTTTCTCTGTCGTACAGTTCTGGTAATATCGCGGGTGGTTTGCGTATAGACAGCGGGAGAATGAGGTCGAAGCGTGGCGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGGTTAATATTTCAGACTTTTTCCTGTATTTAAATCAGAAAACTAACTTCCCTCAGTATGCTTTTGGTTGTTATTCATCATTTACTAATAACAAATTTGTTCAACTTATTCATGCCAGTCACTGTAATCGGATTGTATGGGCTATACATACATACATACATACATACATACATACATACATACATGTATATATACACACACATCTTTTGTTATTTCTTCACTTTTTGCTCGGATTTTGGCAAGTTTACTGGATAATTTTTTTCATCTTTTTATATCTTATTCTTTTCAAGAAATATTTTAATGAATGATCTTATTCTTTTATTATAATTTCTATTAAAAAATAAAATAACATAGCAATAAATATAATAATATTTTAACTTAAAATTTAATACTAAATAAATAAATAAATATATATATATATAAATATTTGTCTTAATATTTGTGTGTGTAAAGTGATGGGACCAGGAATTTGGCTAGATGGGAGACAAGGACGCGAAATGCAATTTCGAGAAATCGCTCCTTGCTCTTTTACTCATTATAATCTGCAGCTTTTGAAAACACTATCAATTTTTTATTATAACAATTTGATCCTGGGTCCCGGCAGAATTGGCACAGAACATGAGAAGTTTGGATTCCAGATTGGAAGTTTACGTCCCATGACGTACGAGCAAATAAGTGGGTTGCTAAACGGGCTTGCAGAGAGATTTAGTTGGGAAAAAGTAATGGAAGGCAGCGACATTATAGGGCTCCGGCAGGTAAACCGCCCAGACCCTGCCAGTAAATACTGGCGAGTAGCACCTATGCACTGCCTCTGACCTGTTTATAAGTAGATGCTCTGTTGGTATCCTTCTAGAAGAAATGTTTCCATTTTCTTCCTAACACGAGATTATTATGGACCAGGGTATGCAAGGTATATCACTGGAACCTGGGGGTCAATTTGAACTCAGTGGTGCCCCTCTTCAAACTTTGCATCAAACCTGTGCTGAAGTTAATTCACACCTTTACCAGGTTTGTGCCACGTTGCTCCTGTATGGCGTGCACACATTTTCTGTTCATTTTCAATGCCTATCCTCCCTTTTTTCAAAAGTTCCAACACCATTTTGAGAGATTTCTCACAGTTAATTATGGGGACCTGAGAGAATAACCCCTAAAATTATATTCCCTGCCCTACGTTGTTTTATATATATATCTTAATTTTCTTTTACTTAGTAAATATATATTGTAATATTTTGATTCCATTTTTTAGAAATTACCTTAAATATTACATTTACTTTTTGTTTAGGAAATGTTATTAAATTTTTAAATATGAATTATATTAAAAAACATAAAAATCATTCAAAACGAATGATATTTTAAAAGAAAATAAATTAAAAATGAGAAAAATGTATGTGAAAAATCTCAATAGAATTAATGAATAATTTCACCAACATAAGAAGAATCAAAATAGAAGACGTCACATCTCCAGTCAATAGAATAAATGAATAATTTCACCAACATAAGAAGAATCAAAATAGAAGACGTCACATCTCCAGTTTTAGTTGTAAATTATCTGGTTGACTTCTTTAGGCGAAAGCTGCAGCTGAGGAGATGGGTATTGGATTTTTAGGAATTGGATTCCAGCCCAAATGGAGACGTGACGAGATACCAATGATGCCCAAGGTATAACTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATGTTTGGAACGTTTTACACGAATTATTATTTGGTCTCAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTGGTTCACTTGGGCTGGACATGATGCTTAGGACATGCACCGTGCAGGTTAATCTAGATTTACGTCCTCTAAATTAGGTCAATAAGTCTACTTTTTCTAAATCAGATTAATTGTCGAATAATTCATGTAGGTTAATCTTGATTTTGATTCCGAAGCCGACATGATCAGAAAGTTCCGAGTGGGTCTTGCTCTTCAACCTGTGAGAAACGTTGTCTTTTTAAATATGAATTATGTTTTAAAAGTTACGTCTGTTCAATGTGTCTGAATACTTGTATAAGTGAACAGATAGCAACTGCTCTGTTTGCCAATTCACCATTTACCGAAGGAAAGCCAAATGGTTATTTGAGCATGAGAAGGTATGCTCTTGTTGGTTTTCATATAAATTTTTTTCTCTGTTTTTCTCCCTCCCTACCACATCAATCCATGCTCATGTTGCTTTTCAGCCAGATATGGACCGATACTGATAATAGCCGCACTGGTATGCTTCCATTTGTCTTCGATGACTCCTTCGGGTAATCTGCCTCCTCCGGAACTGAGCTCTTTCATTGCAGTGCTTTTCTTTATAAATATTTCCTGGGATTTCTTCTTCCTTAATGTATGACGATGATTAAATTCAGTATTTTCTTTTATAATCTTCCTATGACGTATTGGAGATTATTGTACTTGGTGGCTCATAGCATGCATCATAACTGAAGAATCAAGCTAGGGTGCCCTAGCCTTTGCAACTTCTTGTAATCTTTTTTTTTTTTTTTTTTTTTTTTTTGGNTTTTTACTATAGTTAAGATTTGGTCCCTAGGTTTCAAATTGTTACATTTTTACTCTTGAGATTTCTCACTAATACTCATTTTTTTTGTAATTTATGTAAATTTATTATAATTAGATTAATTAATTGCTACTAATTTTTCATCACTATTGAAATTAATTTAAAAAAGCTGTATCATAATATTTAAACTAATGAATAGAGACTAAACACCAAGGACCAAAAGTAAGCATTAATAGTAAAAATTCAAGGGTAAAAGTGTATAATCCTAAAACCTTGGACTAAATGGAAATTGAACTCAAAACTCAAGGTAAGGTAAGAGCGTAAAATTTTGAAACCTAGGACCCAAATAGAAATCAAACCCGAAAGTCAAGGTAAGAATGTAACATTTTGAAACTTAGAAACTAAATGAAAACTAGACCAAAATGGTAAAATACCCTTTTTATTTTATTGAATTAGCACTTTTTGAAACCTGGAAGAGAGAACCACCAAATTTATTCGAATTCTTTTTAAAAGCTAACACATATGGAAACCCTGTTAATTCTATTTCCCCTTTCTTCCTTGGTTCTTTCCTCTTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGGTCGTTATTCAAACTTGATATTGTCAAAATGCAGATTTGAGAAGTATGTTGATTATGCTCTTGATGTTCCAATGTATTTCATATATCGGAAAAAGAAGTATATTGACTGTTCTGGAATGTCCTTCAGGGTATGCATTTACCCATCATAATCGTCCAATTGCGAAGCTTTCTGTCTATCTCTTCCTTTCTATTCATCTCAATTTTTATTTTTAAGTTATTAACAATCGATACATTATTGGGTTGTCTTTCCACAGGACTTTATGGCAGGAAAGCTCTCTCCAGTTCCTGGAGAATTTCCAACACTTTCTGATTGGGAAAATCACTTGACTACAATATATCCAGAGGTATCCTATGATCAATTTGCTCCAATATTGTGCTTGTTATGACCTAATTATAGGATAAAATTTTCCCATATTACTCAGGTCAGGTTGAAGAGATATTTGGAGATGAGGGGCGGCGATGGGGGCCCTTGGAGGAGAATATGTGCTCTGCCAGCATTTTGGGTAATCTTCATGATATAATCAAGCTATGTAGACCTTTTCTTAGACATGGTTGTAATATGTATTCTAATAACTAAGATGCTTGCTTAAAATTTTGGAGTTCTTTTTGTCTGGGGGGACGGTTCATTATTACTTGATCTATAACTAATCTATGTATAGAATCAGGTGTTTAGAGTACTGCTAATGCTCTCTCTTCAAATGTCAGGTTGGTTTATTATATGACGAAGTTTCCCTGCAAAATGCTTTGGACATAACAGCTGACTGGACACCTGAAGAAAGACAGATGTTGAGGAATGAGGTAATAGGATCTTAGGGGAAACATTTTTTTTTCTTTCTAGTTGCCCATTGCTACTTGTGAGAAGCAGGGCAGACTCCGGCTCCTTCATTTGAACTAGGTTATGAAATTTAAATTGTTGCAATAAATTCTACATGCCTTACTACGGAATAATTTTTTTATCAGGTTCCAATAACCGGTCTCAACACACCATTTCGTGATGGGTTGCTGAAGCACGTTGCTCAAGATGTTGTTAAGTTAGCAAAGGTATGTCTAGCAATATTATTTTCTCTTGTGAGTGCTGGTCGTATTATTGGCACCACTAATGTTGCGACTGAAACAAAAACTTCCCCACTTTTAGGATGGCTTAAAGAGAAGAGGTTTCAAGGAGTCTGGCTTCTTGAATGAGGTGACTGAAGTAGCTAGAACAGGTATGAACAACTTTTAAGTTAATTTTAAGAAACCTGAGTTCTATCTTTTAATTGGCTGAATTCCTTGTTTTGCTTACATATTTGGAGCCCTAAAAAAACACTGCATGGGTTTAATTTAATTACAGGTGTGACACTGGCTGAAAAGCTGTTAGGATTGTATAATGGAAAATGGGGACAAAATGTAGATCCAGTTTTCGAGGAGCTTCTATATTAAGCTGCATCTCTTTTAGTTTTTGAGATTGCTACATGAACAACATATAACTACATGTTCTTGAAACTTGTCCTTAGCTTGATGATGGTATTCATAGTTGTTGTAATTCTGTGATGGCCTTCTTTTGGTTGTGGTAAATTGTAACCCCACAAATAATAATGATGATATTAACCACTAGAGGATGTTCATTGTAGAGAAGCCATCAGCTTACCGAGCCATGAACCTTCTGTTCATCCAAAAGAAATAAAAAAATGCTCCCAATAGTTTTGGC

mRNA sequence

CCAAATTTCTTACAAAAAGGAAAAGCGAATTCGAATTCAGCGCCGGAGGGTTCTACGCTTCCAGAATCGCGCAATGGCTCTGGTTTCACAGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGATGGAGTGGAGGATTTATGGCTTTCAAGCTGTGCACTCATATGGAGTCGTTGAAGAGGAAGGAGAATCTTATTAGTGCGTTTTCTCTGTCGTACAGTTCTGGTAATATCGCGGGTGGTTTGCGTATAGACAGCGGGAGAATGAGGTCGAAGCGTGGCGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGAATTGGCACAGAACATGAGAAGTTTGGATTCCAGATTGGAAGTTTACGTCCCATGACGTACGAGCAAATAAGTGGGTTGCTAAACGGGCTTGCAGAGAGATTTAGTTGGGAAAAAGTAATGGAAGGCAGCGACATTATAGGGCTCCGGCAGGGTATGCAAGGTATATCACTGGAACCTGGGGGTCAATTTGAACTCAGTGGTGCCCCTCTTCAAACTTTGCATCAAACCTGTGCTGAAGTTAATTCACACCTTTACCAGGCGAAAGCTGCAGCTGAGGAGATGGGTATTGGATTTTTAGGAATTGGATTCCAGCCCAAATGGAGACGTGACGAGATACCAATGATGCCCAAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTGGTTCACTTGGGCTGGACATGATGCTTAGGACATGCACCGTGCAGGTTAATCTTGATTTTGATTCCGAAGCCGACATGATCAGAAAGTTCCGAGTGGGTCTTGCTCTTCAACCTATAGCAACTGCTCTGTTTGCCAATTCACCATTTACCGAAGGAAAGCCAAATGGTTATTTGAGCATGAGAAGCCAGATATGGACCGATACTGATAATAGCCGCACTGGTATGCTTCCATTTGTCTTCGATGACTCCTTCGGATTTGAGAAGTATGTTGATTATGCTCTTGATGTTCCAATGTATTTCATATATCGGAAAAAGAAGTATATTGACTGTTCTGGAATGTCCTTCAGGGACTTTATGGCAGGAAAGCTCTCTCCAGTTCCTGGAGAATTTCCAACACTTTCTGATTGGGAAAATCACTTGACTACAATATATCCAGAGGTCAGGTTGAAGAGATATTTGGAGATGAGGGGCGGCGATGGGGGCCCTTGGAGGAGAATATGTGCTCTGCCAGCATTTTGGGTTGGTTTATTATATGACGAAGTTTCCCTGCAAAATGCTTTGGACATAACAGCTGACTGGACACCTGAAGAAAGACAGATGTTGAGGAATGAGGTTCCAATAACCGGTCTCAACACACCATTTCGTGATGGGTTGCTGAAGCACGTTGCTCAAGATGTTGTTAAGTTAGCAAAGGATGGCTTAAAGAGAAGAGGTTTCAAGGAGTCTGGCTTCTTGAATGAGGTGACTGAAGTAGCTAGAACAGGTGTGACACTGGCTGAAAAGCTGTTAGGATTGTATAATGGAAAATGGGGACAAAATGTAGATCCAGTTTTCGAGGAGCTTCTATATTAAGCTGCATCTCTTTTAGTTTTTGAGATTGCTACATGAACAACATATAACTACATGTTCTTGAAACTTGTCCTTAGCTTGATGATGGTATTCATAGTTGTTGTAATTCTGTGATGGCCTTCTTTTGGTTGTGGTAAATTGTAACCCCACAAATAATAATGATGATATTAACCACTAGAGGATGTTCATTGTAGAGAAGCCATCAGCTTACCGAGCCATGAACCTTCTGTTCATCCAAAAGAAATAAAAAAATGCTCCCAATAGTTTTGGC

Coding sequence (CDS)

ATGGCTCTGGTTTCACAGACTGGTTCGGCTCACCGGATTGGTGTAGAGAGTGTACGATGGAGTGGAGGATTTATGGCTTTCAAGCTGTGCACTCATATGGAGTCGTTGAAGAGGAAGGAGAATCTTATTAGTGCGTTTTCTCTGTCGTACAGTTCTGGTAATATCGCGGGTGGTTTGCGTATAGACAGCGGGAGAATGAGGTCGAAGCGTGGCGGTAGGGTGATTGTTGCTACGGGTGCACCGGCCGAGGAAGCAACAGTGGCTACCCAACCGTTGACGAAAGAGGATCTCGTGGCTTATTTAGCTTCTGGATGCAAGCCAAAGGAAAAGTGGAGAATTGGCACAGAACATGAGAAGTTTGGATTCCAGATTGGAAGTTTACGTCCCATGACGTACGAGCAAATAAGTGGGTTGCTAAACGGGCTTGCAGAGAGATTTAGTTGGGAAAAAGTAATGGAAGGCAGCGACATTATAGGGCTCCGGCAGGGTATGCAAGGTATATCACTGGAACCTGGGGGTCAATTTGAACTCAGTGGTGCCCCTCTTCAAACTTTGCATCAAACCTGTGCTGAAGTTAATTCACACCTTTACCAGGCGAAAGCTGCAGCTGAGGAGATGGGTATTGGATTTTTAGGAATTGGATTCCAGCCCAAATGGAGACGTGACGAGATACCAATGATGCCCAAGGGACGATACAAGATTATAGAGAATTACATGCCCAAAGTTGGTTCACTTGGGCTGGACATGATGCTTAGGACATGCACCGTGCAGGTTAATCTTGATTTTGATTCCGAAGCCGACATGATCAGAAAGTTCCGAGTGGGTCTTGCTCTTCAACCTATAGCAACTGCTCTGTTTGCCAATTCACCATTTACCGAAGGAAAGCCAAATGGTTATTTGAGCATGAGAAGCCAGATATGGACCGATACTGATAATAGCCGCACTGGTATGCTTCCATTTGTCTTCGATGACTCCTTCGGATTTGAGAAGTATGTTGATTATGCTCTTGATGTTCCAATGTATTTCATATATCGGAAAAAGAAGTATATTGACTGTTCTGGAATGTCCTTCAGGGACTTTATGGCAGGAAAGCTCTCTCCAGTTCCTGGAGAATTTCCAACACTTTCTGATTGGGAAAATCACTTGACTACAATATATCCAGAGGTCAGGTTGAAGAGATATTTGGAGATGAGGGGCGGCGATGGGGGCCCTTGGAGGAGAATATGTGCTCTGCCAGCATTTTGGGTTGGTTTATTATATGACGAAGTTTCCCTGCAAAATGCTTTGGACATAACAGCTGACTGGACACCTGAAGAAAGACAGATGTTGAGGAATGAGGTTCCAATAACCGGTCTCAACACACCATTTCGTGATGGGTTGCTGAAGCACGTTGCTCAAGATGTTGTTAAGTTAGCAAAGGATGGCTTAAAGAGAAGAGGTTTCAAGGAGTCTGGCTTCTTGAATGAGGTGACTGAAGTAGCTAGAACAGGTGTGACACTGGCTGAAAAGCTGTTAGGATTGTATAATGGAAAATGGGGACAAAATGTAGATCCAGTTTTCGAGGAGCTTCTATATTAA

Protein sequence

MALVSQTGSAHRIGVESVRWSGGFMAFKLCTHMESLKRKENLISAFSLSYSSGNIAGGLRIDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENYMPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY

Homology

[Close]

BLAST of CmaCh02G014540 vs. ExPASy Swiss-Prot
Match: Q1W2L8 (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=GSH1 PE=2 SV=2)

HSP 1 Score: 842.0 bits (2174), Expect = 3.6e-243
Identity = 402/525 (76.57%), Postives = 452/525 (86.10%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKLCTHMESLKRKENLISAFSLSYSSGNIAGGLR 60
           MAL+SQ GS+H I  E ++   G  +  + ++ME LK K+      S S +S     G+ 
Sbjct: 1   MALMSQAGSSHCIYSEKMKCISGHSS--ITSNMEMLKMKDICFGNIS-SRNSSKPMQGIY 60

Query: 61  IDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKF 120
           +D   +  +RG   IVA   P E+A VA +PLTKEDLVAYLASGCK KEKWRIGTEHEKF
Sbjct: 61  LDRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKF 120

Query: 121 GFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGA 180
           GF+ G+LRPM YEQI+ LLNG+AERF WEKVMEG +IIGL+QG Q ISLEPGGQFELSGA
Sbjct: 121 GFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGA 180

Query: 181 PLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENYMP 240
           PL+TLHQTCAEVNSHLYQ KA AEEMGIGFLG GFQPKW   +IP+MPKGRY+I+ NYMP
Sbjct: 181 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMP 240

Query: 241 KVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNGYL 300
           KVGSLGLDMM RTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNGYL
Sbjct: 241 KVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 300

Query: 301 SMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDF 360
           SMRS IWTDTDN+R GMLPFVFDDSFGFE+YVDYALDVPMYF+YRKKKYIDC+GMSFRDF
Sbjct: 301 SMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDF 360

Query: 361 MAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLY 420
           M GKLSP+PG++PTL+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LY
Sbjct: 361 MNGKLSPIPGDYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 420

Query: 421 DEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKRRG 480
           DEVSLQ  LD+T+DWT EER+MLRN+VP +GL TPFRDGLLKHVAQDVVKLAK+GL+RRG
Sbjct: 421 DEVSLQTVLDMTSDWTAEEREMLRNKVPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRG 480

Query: 481 FKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY 526
           +KE+GFLNEVTEV RTGVT AEKLL LY+GKWG++VDPVFEELLY
Sbjct: 481 YKETGFLNEVTEVVRTGVTPAEKLLELYHGKWGRSVDPVFEELLY 522

[Close]

BLAST of CmaCh02G014540 vs. ExPASy Swiss-Prot
Match: O22493 (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=GSH1 PE=2 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 1.7e-237
Identity = 392/525 (74.67%), Postives = 441/525 (84.00%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKLCTHMESLKRKENLISAFSLSYSSGNIAGGLR 60
           MAL+SQ GS+H I  E VR   G  +  +  +M+  + +E        S ++     G  
Sbjct: 1   MALMSQAGSSHCIYSEKVRCISGHRS--IINNMDMFRMREICFGVDISSRNASRRVQGNY 60

Query: 61  IDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKF 120
           ++   + S+RG   IVA   P E+A VA +PLTKEDLV YLASGCK KEKWRIGTEHEKF
Sbjct: 61  LNHIGVGSRRGDLTIVAASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKF 120

Query: 121 GFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGA 180
           GF+ G+LRPM Y+QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGA
Sbjct: 121 GFEFGTLRPMKYDQIADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGA 180

Query: 181 PLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENYMP 240
           PL+TLHQTCAEVNSHLYQ KA AEEMGIGFLG GFQPKW   +IP+MPKGRY+II NYMP
Sbjct: 181 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEIIRNYMP 240

Query: 241 KVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNGYL 300
           KVGSLGLDMM RTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNGYL
Sbjct: 241 KVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 300

Query: 301 SMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDF 360
           S RS IWTDTDN+R GMLPFVFDDSFGFE+YVDYALDVPMYF+YRKKKY+DC+G+SFRDF
Sbjct: 301 SKRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYVDCTGLSFRDF 360

Query: 361 MAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLY 420
           M GKL P+PGE+PTL+DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVG+LY
Sbjct: 361 MNGKLPPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 420

Query: 421 DEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKRRG 480
           DE SLQ+ LD+T DWT EER MLRN+VP +GL TPFRDGLL HVAQDVVKLAK+GL+RRG
Sbjct: 421 DEGSLQSVLDMTFDWTAEERDMLRNKVPKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRG 480

Query: 481 FKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY 526
           FKE+GFLNEV EV +TGVT AEKLL LY+GKWGQ+VDP+FEELLY
Sbjct: 481 FKETGFLNEVAEVVKTGVTPAEKLLELYHGKWGQSVDPIFEELLY 523

[Close]

BLAST of CmaCh02G014540 vs. ExPASy Swiss-Prot
Match: O23736 (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea OX=3707 GN=GSH1 PE=1 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 5.5e-236
Identity = 388/525 (73.90%), Postives = 442/525 (84.19%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKLCTHMESLKRKENLISAFSLSYSSGNIAGGLR 60
           MAL+SQ G A+ +    V    G      C ++  L+ KE  +S++S + S+ ++     
Sbjct: 1   MALLSQAGGAYTVPSGHVSSRTGTKTVSGCVNV--LRMKETYVSSYSRTLSTKSML---- 60

Query: 61  IDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHEKF 120
                 RSKRG ++IVA   P EEA VAT+PLT+EDL+AYLASGCK KEKWRIGTEHEKF
Sbjct: 61  -----KRSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKSKEKWRIGTEHEKF 120

Query: 121 GFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELSGA 180
           GF++ +LRPM Y+QI+ LLN +AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELSGA
Sbjct: 121 GFEVNTLRPMKYDQIAELLNSIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGA 180

Query: 181 PLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENYMP 240
           PL+TLHQTCAEVNSHLYQ KA AEEMGIGFLG+GFQPKWRR++IP MPKGRY I+ NYMP
Sbjct: 181 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPTMPKGRYDIMRNYMP 240

Query: 241 KVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNGYL 300
           KVGSLGLDMMLRTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNG+L
Sbjct: 241 KVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFL 300

Query: 301 SMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDF 360
           SMRS IWTDTD  RTGMLPFVFDDSFGFE+YVDYALDVPMYF YR  KY+DC+GM+FR F
Sbjct: 301 SMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNGKYVDCTGMTFRQF 360

Query: 361 MAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGLLY 420
           +AGKL  +PGE PT +DWENHLTTI+PEVRLKRY+EMRG DGGPWRR+CALPAFWVGLLY
Sbjct: 361 LAGKLPCLPGELPTYNDWENHLTTIFPEVRLKRYMEMRGADGGPWRRLCALPAFWVGLLY 420

Query: 421 DEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKRRG 480
           DE  LQ+ LD+TADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV+KLAKDGL+RRG
Sbjct: 421 DEDVLQSVLDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRG 480

Query: 481 FKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY 526
           +KE GFLN VTEV RTGVT AE LL +YNG+WGQ+VDPVF+ELLY
Sbjct: 481 YKEVGFLNAVTEVVRTGVTPAENLLEMYNGEWGQSVDPVFQELLY 514

[Close]

BLAST of CmaCh02G014540 vs. ExPASy Swiss-Prot
Match: P46309 (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSH1 PE=1 SV=2)

HSP 1 Score: 816.6 bits (2108), Expect = 1.6e-235
Identity = 392/527 (74.38%), Postives = 446/527 (84.63%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKL--CTHMESLKRKENLISAFSLSYSSGNIAGG 60
           MAL+SQ G ++ +    V    G  A       +++ L+ KE    AF  SYS       
Sbjct: 1   MALLSQAGGSYTVVPSGVCSKAGTKAVVSGGVRNLDVLRMKE----AFGSSYSRSLSTKS 60

Query: 61  LRIDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHE 120
           + + S + RSKRG ++IVA   P EEA VAT+PLT+EDL+AYLASGCK K+K+RIGTEHE
Sbjct: 61  MLLHSVK-RSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHE 120

Query: 121 KFGFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELS 180
           KFGF++ +LRPM Y+QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELS
Sbjct: 121 KFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELS 180

Query: 181 GAPLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENY 240
           GAPL+TLHQTCAEVNSHLYQ KA AEEMGIGFLGIGFQPKWRR++IP+MPKGRY I+ NY
Sbjct: 181 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNY 240

Query: 241 MPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNG 300
           MPKVG+LGLDMMLRTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNG
Sbjct: 241 MPKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNG 300

Query: 301 YLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFR 360
           +LSMRS IWTDTD  RTGMLPFVFDDSFGFE+YVDYALDVPMYF YRK KYIDC+GM+FR
Sbjct: 301 FLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFR 360

Query: 361 DFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGL 420
            F+AGKL  +PGE P+ +DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGL
Sbjct: 361 QFLAGKLPCLPGELPSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGL 420

Query: 421 LYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKR 480
           LYD+ SLQ  LD+TADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV+KLAKDGL+R
Sbjct: 421 LYDDDSLQAILDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLER 480

Query: 481 RGFKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY 526
           RG+KE+GFLN V EV RTGVT AEKLL +YNG+WGQ+VDPVFEELLY
Sbjct: 481 RGYKEAGFLNAVDEVVRTGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522

[Close]

BLAST of CmaCh02G014540 vs. ExPASy Swiss-Prot
Match: Q9ZNX6 (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula OX=3880 GN=GSH1 PE=2 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 1.2e-230
Identity = 379/488 (77.66%), Postives = 427/488 (87.50%), Query Frame = 0


Query: 38  RKENLISAFSLSYSSGNIAGGLRIDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDL 97
           RK ++ + F  S+SS N     RI    + S    R IVA   P E+A VAT+PLTK+DL
Sbjct: 27  RKTSISNTF--SFSSKNSLSFKRI----LTSGGSRRFIVAASPPTEDAVVATEPLTKQDL 86

Query: 98  VAYLASGCKPKEKWRIGTEHEKFGFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDI 157
           + YLASGCK K+KWRIGTEHEKFGF++GSLRPM YEQIS LLNG+AERF W+KVMEG +I
Sbjct: 87  IDYLASGCKTKDKWRIGTEHEKFGFELGSLRPMKYEQISELLNGIAERFDWDKVMEGDNI 146

Query: 158 IGLRQGMQGISLEPGGQFELSGAPLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQP 217
           IGL+QG Q ISLEPGGQFELSGAPL+TLHQTCAEVNSHLYQ KA AEEMGIGFLGIGFQP
Sbjct: 147 IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQP 206

Query: 218 KWRRDEIPMMPKGRYKIIENYMPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLA 277
           KW R +IPMMPKGRY+I++ YMPKVGSLGLDMM RTCTVQVNLDF SEADMIRKFR GLA
Sbjct: 207 KWERKDIPMMPKGRYEIMKKYMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLA 266

Query: 278 LQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALD 337
           LQPIATALFANSPFT+GKPNG++SMRS IWTDTD  RTGMLPFVFDDSFGFE+YVD+ALD
Sbjct: 267 LQPIATALFANSPFTDGKPNGFVSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDFALD 326

Query: 338 VPMYFIYRKKKYIDCSGMSFRDFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEM 397
           VPMYF+YRKKKYIDC+GM+FRDF+AGKL  +PGE PTL+DWENHLTTI+PEVRLKRYLEM
Sbjct: 327 VPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEM 386

Query: 398 RGGDGGPWRRICALPAFWVGLLYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFR 457
           RG DGGPWRR+CALPAFWVG+LYDEVSLQ  LD+TADWT EER+MLRN+V +TGL TPFR
Sbjct: 387 RGADGGPWRRLCALPAFWVGILYDEVSLQRVLDMTADWTLEEREMLRNKVTVTGLKTPFR 446

Query: 458 DGLLKHVAQDVVKLAKDGLKRRGFKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVD 517
           DGLLKHVA++V++LAKDGL+RRGFKESGFLN V EV RTGVT AE+LL LY+GKW Q+VD
Sbjct: 447 DGLLKHVAEEVLELAKDGLERRGFKESGFLNAVAEVVRTGVTPAERLLELYHGKWEQSVD 506

Query: 518 PVFEELLY 526
            VF+ELLY
Sbjct: 507 HVFDELLY 508

[Close]

BLAST of CmaCh02G014540 vs. TAIR 10
Match: AT4G23100.1 (glutamate-cysteine ligase )

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-236
Identity = 392/527 (74.38%), Postives = 446/527 (84.63%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKL--CTHMESLKRKENLISAFSLSYSSGNIAGG 60
           MAL+SQ G ++ +    V    G  A       +++ L+ KE    AF  SYS       
Sbjct: 1   MALLSQAGGSYTVVPSGVCSKAGTKAVVSGGVRNLDVLRMKE----AFGSSYSRSLSTKS 60

Query: 61  LRIDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHE 120
           + + S + RSKRG ++IVA   P EEA VAT+PLT+EDL+AYLASGCK K+K+RIGTEHE
Sbjct: 61  MLLHSVK-RSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHE 120

Query: 121 KFGFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELS 180
           KFGF++ +LRPM Y+QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELS
Sbjct: 121 KFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELS 180

Query: 181 GAPLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENY 240
           GAPL+TLHQTCAEVNSHLYQ KA AEEMGIGFLGIGFQPKWRR++IP+MPKGRY I+ NY
Sbjct: 181 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNY 240

Query: 241 MPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNG 300
           MPKVG+LGLDMMLRTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNG
Sbjct: 241 MPKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNG 300

Query: 301 YLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFR 360
           +LSMRS IWTDTD  RTGMLPFVFDDSFGFE+YVDYALDVPMYF YRK KYIDC+GM+FR
Sbjct: 301 FLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFR 360

Query: 361 DFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGL 420
            F+AGKL  +PGE P+ +DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGL
Sbjct: 361 QFLAGKLPCLPGELPSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGL 420

Query: 421 LYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKR 480
           LYD+ SLQ  LD+TADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV+KLAKDGL+R
Sbjct: 421 LYDDDSLQAILDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLER 480

Query: 481 RGFKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY 526
           RG+KE+GFLN V EV RTGVT AEKLL +YNG+WGQ+VDPVFEELLY
Sbjct: 481 RGYKEAGFLNAVDEVVRTGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522

[Close]

BLAST of CmaCh02G014540 vs. TAIR 10
Match: AT4G23100.3 (glutamate-cysteine ligase )

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-236
Identity = 392/527 (74.38%), Postives = 446/527 (84.63%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKL--CTHMESLKRKENLISAFSLSYSSGNIAGG 60
           MAL+SQ G ++ +    V    G  A       +++ L+ KE    AF  SYS       
Sbjct: 1   MALLSQAGGSYTVVPSGVCSKAGTKAVVSGGVRNLDVLRMKE----AFGSSYSRSLSTKS 60

Query: 61  LRIDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHE 120
           + + S + RSKRG ++IVA   P EEA VAT+PLT+EDL+AYLASGCK K+K+RIGTEHE
Sbjct: 61  MLLHSVK-RSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHE 120

Query: 121 KFGFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELS 180
           KFGF++ +LRPM Y+QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELS
Sbjct: 121 KFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELS 180

Query: 181 GAPLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENY 240
           GAPL+TLHQTCAEVNSHLYQ KA AEEMGIGFLGIGFQPKWRR++IP+MPKGRY I+ NY
Sbjct: 181 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNY 240

Query: 241 MPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNG 300
           MPKVG+LGLDMMLRTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNG
Sbjct: 241 MPKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNG 300

Query: 301 YLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFR 360
           +LSMRS IWTDTD  RTGMLPFVFDDSFGFE+YVDYALDVPMYF YRK KYIDC+GM+FR
Sbjct: 301 FLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFR 360

Query: 361 DFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGL 420
            F+AGKL  +PGE P+ +DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGL
Sbjct: 361 QFLAGKLPCLPGELPSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGL 420

Query: 421 LYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAKDGLKR 480
           LYD+ SLQ  LD+TADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV+KLAKDGL+R
Sbjct: 421 LYDDDSLQAILDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLER 480

Query: 481 RGFKESGFLNEVTEVARTGVTLAEKLLGLYNGKWGQNVDPVFEELLY 526
           RG+KE+GFLN V EV RTGVT AEKLL +YNG+WGQ+VDPVFEELLY
Sbjct: 481 RGYKEAGFLNAVDEVVRTGVTPAEKLLEMYNGEWGQSVDPVFEELLY 522

[Close]

BLAST of CmaCh02G014540 vs. TAIR 10
Match: AT4G23100.2 (glutamate-cysteine ligase )

HSP 1 Score: 729.9 bits (1883), Expect = 1.4e-210
Identity = 351/475 (73.89%), Postives = 399/475 (84.00%), Query Frame = 0


Query: 1   MALVSQTGSAHRIGVESVRWSGGFMAFKL--CTHMESLKRKENLISAFSLSYSSGNIAGG 60
           MAL+SQ G ++ +    V    G  A       +++ L+ KE    AF  SYS       
Sbjct: 1   MALLSQAGGSYTVVPSGVCSKAGTKAVVSGGVRNLDVLRMKE----AFGSSYSRSLSTKS 60

Query: 61  LRIDSGRMRSKRGGRVIVATGAPAEEATVATQPLTKEDLVAYLASGCKPKEKWRIGTEHE 120
           + + S + RSKRG ++IVA   P EEA VAT+PLT+EDL+AYLASGCK K+K+RIGTEHE
Sbjct: 61  MLLHSVK-RSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHE 120

Query: 121 KFGFQIGSLRPMTYEQISGLLNGLAERFSWEKVMEGSDIIGLRQGMQGISLEPGGQFELS 180
           KFGF++ +LRPM Y+QI+ LLNG+AERF WEKVMEG  IIGL+QG Q ISLEPGGQFELS
Sbjct: 121 KFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELS 180

Query: 181 GAPLQTLHQTCAEVNSHLYQAKAAAEEMGIGFLGIGFQPKWRRDEIPMMPKGRYKIIENY 240
           GAPL+TLHQTCAEVNSHLYQ KA AEEMGIGFLGIGFQPKWRR++IP+MPKGRY I+ NY
Sbjct: 181 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNY 240

Query: 241 MPKVGSLGLDMMLRTCTVQVNLDFDSEADMIRKFRVGLALQPIATALFANSPFTEGKPNG 300
           MPKVG+LGLDMMLRTCTVQVNLDF SEADMIRKFR GLALQPIATALFANSPFTEGKPNG
Sbjct: 241 MPKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNG 300

Query: 301 YLSMRSQIWTDTDNSRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFR 360
           +LSMRS IWTDTD  RTGMLPFVFDDSFGFE+YVDYALDVPMYF YRK KYIDC+GM+FR
Sbjct: 301 FLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFR 360

Query: 361 DFMAGKLSPVPGEFPTLSDWENHLTTIYPEVRLKRYLEMRGGDGGPWRRICALPAFWVGL 420
            F+AGKL  +PGE P+ +DWENHLTTI+PEVRLKRYLEMRG DGGPWRR+CALPAFWVGL
Sbjct: 361 QFLAGKLPCLPGELPSYNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGL 420

Query: 421 LYDEVSLQNALDITADWTPEERQMLRNEVPITGLNTPFRDGLLKHVAQDVVKLAK 474
           LYD+ SLQ  LD+TADWTP ER+MLRN+VP+TGL TPFRDGLLKHVA+DV+KLAK
Sbjct: 421 LYDDDSLQAILDLTADWTPAEREMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAK 470

The following BLAST results are available for this feature:

BLAST of CmaCh02G014540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1W2L8	3.6e-243	76.57	Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=GSH1 P...	[more]
O22493	1.7e-237	74.67	Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=GSH...	[more]
O23736	5.5e-236	73.90	Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea OX=3707 GN=GSH1 PE=...	[more]
P46309	1.6e-235	74.38	Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSH...	[more]
Q9ZNX6	1.2e-230	77.66	Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula OX=3880 GN=GSH1...	[more]

BLAST of CmaCh02G014540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23100.1	1.1e-236	74.38	glutamate-cysteine ligase	[more]
AT4G23100.3	1.1e-236	74.38	glutamate-cysteine ligase	[more]
AT4G23100.2	1.4e-210	73.89	glutamate-cysteine ligase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035434	Glutamate--cysteine ligase, bacteria and plant	PIRSF	PIRSF017901	GCL	coord: 80..525 e-value: 2.3E-194 score: 644.1
IPR035434	Glutamate--cysteine ligase, bacteria and plant	PANTHER	PTHR34378	GLUTAMATE--CYSTEINE LIGASE, CHLOROPLASTIC	coord: 1..525
IPR011556	Glutamate--cysteine ligase, plant-type	TIGRFAM	TIGR01436	TIGR01436	coord: 91..525 e-value: 2.3E-228 score: 756.0
None	No IPR available	GENE3D	3.30.590.20		coord: 77..525 e-value: 1.9E-178 score: 595.8
None	No IPR available	PANTHER	PTHR34378:SF5	GLUTAMATE--CYSTEINE LIGASE	coord: 1..525
IPR006336	Glutamate--cysteine ligase, GCS2	PFAM	PF04107	GCS2	coord: 140..435 e-value: 4.8E-93 score: 311.7
IPR014746	Glutamine synthetase/guanido kinase, catalytic domain	SUPERFAMILY	55931	Glutamine synthetase/guanido kinase	coord: 103..523

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G014540.1	CmaCh02G014540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006750	glutathione biosynthetic process
biological_process	GO:0042398	cellular modified amino acid biosynthetic process
molecular_function	GO:0004357	glutamate-cysteine ligase activity
molecular_function	GO:0003824	catalytic activity