CmaCh02G011610 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh02G011610 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 1.3e-255
Identity = 441/493 (89.45%), Postives = 475/493 (96.35%), Query Frame = 0

Query: 8   KKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGI 67
           K+PKTKIVCTLGPASRSVPM+EKLL+AGMNVARFNFSHGSH+YHQET+DNLR AME+TGI
Sbjct: 16  KRPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGSHDYHQETIDNLRQAMESTGI 75

Query: 68  LCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKP 127
           LCAVMLDTKGPEIRTGFLKD KP+QL QGQEITISTDYS+KGDE+MICMSYKKLAEDVKP
Sbjct: 76  LCAVMLDTKGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKP 135

Query: 128 GSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKED 187
            SVILC+DG I+F+VLSCDKE+GL RCRCEN+AVLGERKNVNLPGVIVDLPTLT+KDK+D
Sbjct: 136 QSVILCADGQITFTVLSCDKENGLDRCRCENTAVLGERKNVNLPGVIVDLPTLTDKDKDD 195

Query: 188 ILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANS 247
           IL WGVPN+IDMIALSFVRKGSDLVEVRKLLG+HAK+ILLMSKVENQEGVANFDDIL NS
Sbjct: 196 ILNWGVPNHIDMIALSFVRKGSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDDILLNS 255

Query: 248 DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD 307
           DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD
Sbjct: 256 DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD 315

Query: 308 VANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMS 367
           VANAVLDGTDCVMLSGETAAGAYP+LAV TMAKIC+EAEST++Y DVFKRIM ++PVPMS
Sbjct: 316 VANAVLDGTDCVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPDVFKRIMSNAPVPMS 375

Query: 368 PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSC 427
           PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPG PILSVVVPEIKTDSFDW+C
Sbjct: 376 PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSVVVPEIKTDSFDWTC 435

Query: 428 SDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALHR 487
           SDE+PARHSLIFRGLVPVL   SA++SH E+TEEA++FA+QHAK KGLCK GDSVVALHR
Sbjct: 436 SDESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTKGLCKQGDSVVALHR 495

Query: 488 VGTASVIKILTVK 501
           VGTASVIKI+TVK
Sbjct: 496 VGTASVIKIVTVK 508

BLAST of CmaCh02G011610 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 5.8e-243
Identity = 422/497 (84.91%), Postives = 458/497 (92.15%), Query Frame = 0

Query: 4   LVIEKKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAME 63
           + +E++PKTKIVCTLGPASRSVPMVEKLL AGM+VARFNFSHGS+EYHQETLDNLR AM 
Sbjct: 1   MAMEQRPKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAML 60

Query: 64  NTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAE 123
           NTG+LCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDY LKGDE  ICMSYKKLA+
Sbjct: 61  NTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQ 120

Query: 124 DVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEK 183
           DV PG VILC+DGTIS  VLSCDKE G VRCRCEN+++LGERKNVNLPGV+VDLPTLTEK
Sbjct: 121 DVNPGMVILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEK 180

Query: 184 DKEDILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDI 243
           DK+DILEWGVPN IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFDDI
Sbjct: 181 DKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDI 240

Query: 244 LANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRA 303
           L NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCN  GKPVVTATQMLESMIKSPRPTRA
Sbjct: 241 LINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRA 300

Query: 304 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSP 363
           EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGD+FKRIM H+ 
Sbjct: 301 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAA 360

Query: 364 VPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSF 423
           VPMSP+ESLASSAVRTA S++A L++VLTRGGSTA+LVAKYRPG PILSVVVPEI +DSF
Sbjct: 361 VPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSF 420

Query: 424 DWSCSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVV 483
           DW+CS+EAPARHSLI+RGLVPVL   SA++S  E+TEE +EFA ++ K K LCK GDSVV
Sbjct: 421 DWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVV 480

Query: 484 ALHRVGTASVIKILTVK 501
           AL R G A VIKILTVK
Sbjct: 481 ALFRTGNAIVIKILTVK 497

BLAST of CmaCh02G011610 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 3.8e-226
Identity = 388/491 (79.02%), Postives = 438/491 (89.21%), Query Frame = 0

Query: 10  PKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILC 69
           PKTKIVCTLGPASRSV M EKLL+AGMNVARFNFSHG+H+YHQETL+NL+ AM NTGILC
Sbjct: 21  PKTKIVCTLGPASRSVEMTEKLLRAGMNVARFNFSHGTHDYHQETLNNLKTAMHNTGILC 80

Query: 70  AVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 129
           AVMLDTKGPEIRTGFLKDGKPIQL +GQE+TI+TDY +KGD  MI MSYKKL   +KPG+
Sbjct: 81  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVTITTDYDIKGDPEMISMSYKKLPVHLKPGN 140

Query: 130 VILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDIL 189
            ILCSDGTI+ +VLSCD ++G VRCRCEN+A LGERKNVNLPGV+VDLPTLTEKDKEDIL
Sbjct: 141 TILCSDGTITLTVLSCDPDAGTVRCRCENTATLGERKNVNLPGVVVDLPTLTEKDKEDIL 200

Query: 190 EWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 249
            WGVPN IDMIALSFVRKGSDLV VRK+LG HAK+I LMSKVENQEGV NFD+IL  +DA
Sbjct: 201 GWGVPNKIDMIALSFVRKGSDLVNVRKVLGPHAKNIQLMSKVENQEGVLNFDEILRETDA 260

Query: 250 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 309
           FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 261 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVA 320

Query: 310 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMSPL 369
           NAVLDGTDCVMLSGE+AAGAYPELAV+ MA+IC+EAES+L+YG +FK ++  +P+PMSPL
Sbjct: 321 NAVLDGTDCVMLSGESAAGAYPELAVKIMARICIEAESSLDYGAIFKEMIRSTPLPMSPL 380

Query: 370 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSD 429
           ESLASSAVRTAN AKA LI+VLTRGGSTAKLVAKYRP  PILSVVVP + TDSFDW+CSD
Sbjct: 381 ESLASSAVRTANKAKAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLSTDSFDWTCSD 440

Query: 430 EAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALHRVG 489
           E PARHSLI+RGL+P+L   SAK++ AE+TE  +E A++ A  + LCK GD+VVALHR+G
Sbjct: 441 ETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKSATERALCKPGDAVVALHRIG 500

Query: 490 TASVIKILTVK 501
            ASVIKI  VK
Sbjct: 501 AASVIKICIVK 511

BLAST of CmaCh02G011610 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.1e-223
Identity = 383/491 (78.00%), Postives = 439/491 (89.41%), Query Frame = 0

Query: 10  PKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILC 69
           PKTKIVCTLGP+SR+VPM+EKLL+AGMNVARFNFSHG+HEYHQETLDNL+ AM+NT ILC
Sbjct: 20  PKTKIVCTLGPSSRTVPMLEKLLRAGMNVARFNFSHGTHEYHQETLDNLKIAMQNTQILC 79

Query: 70  AVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 129
           AVMLDTKGPEIRTGFL DGKPIQL +GQEIT+STDY++KG+E MI MSYKKL  D+KPG+
Sbjct: 80  AVMLDTKGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGNEEMISMSYKKLVMDLKPGN 139

Query: 130 VILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDIL 189
            ILC+DGTI+ +VLSCD  SG VRCRCEN+A LGERKNVNLPGV+VDLPTLTEKDKEDIL
Sbjct: 140 TILCADGTITLTVLSCDPPSGTVRCRCENTATLGERKNVNLPGVVVDLPTLTEKDKEDIL 199

Query: 190 EWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 249
           EWGVPNNIDMIALSFVRKGSDLV VRK+LG HAK I LMSKVENQEGV NFD+IL  +D+
Sbjct: 200 EWGVPNNIDMIALSFVRKGSDLVNVRKVLGPHAKRIQLMSKVENQEGVINFDEILRETDS 259

Query: 250 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 309
           FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GK VVTATQMLESMIKSP PTRAEATDVA
Sbjct: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKAVVTATQMLESMIKSPAPTRAEATDVA 319

Query: 310 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMSPL 369
           NAVLDGTDCVMLSGE+AAGAYPELAV+ M++IC+EAES+L+   +FK ++  +P+PMSPL
Sbjct: 320 NAVLDGTDCVMLSGESAAGAYPELAVKIMSRICIEAESSLDNEAIFKEMIRCTPLPMSPL 379

Query: 370 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSD 429
           ESLASSAVRTAN A+A LI+VLTRGGSTAKLVAKYRP  PILSVVVP + TDSFDWS SD
Sbjct: 380 ESLASSAVRTANKARAKLIVVLTRGGSTAKLVAKYRPAVPILSVVVPVLTTDSFDWSISD 439

Query: 430 EAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALHRVG 489
           E PARHSL++RGL+P+L   SAK++ +E+TE  +E A++ A  +GLCK GD+VVALHR+G
Sbjct: 440 ETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSAVTRGLCKPGDAVVALHRIG 499

Query: 490 TASVIKILTVK 501
           +ASVIKI  VK
Sbjct: 500 SASVIKICVVK 510

BLAST of CmaCh02G011610 vs. ExPASy Swiss-Prot
Match: Q54RF5 (Pyruvate kinase OS=Dictyostelium discoideum OX=44689 GN=pyk PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 3.2e-124
Identity = 249/498 (50.00%), Postives = 333/498 (66.87%), Query Frame = 0

Query: 11  KTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILCA 70
           +TKIVCT+GP + S   + KL++ GMNV R NFSHG+H+YH + + N+R+AME TG + A
Sbjct: 21  RTKIVCTIGPKTMSEEALIKLIETGMNVCRLNFSHGTHDYHGQVIKNVRSAMEKTGKIIA 80

Query: 71  VMLDTKGPEIRTGFLKDG-KPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 130
           +MLDTKGPEIRTG ++D    + L  GQEI + T+ +  G    I + YK L + VK G 
Sbjct: 81  IMLDTKGPEIRTGKIEDRCGYVDLFVGQEILVDTNMNQPGTSFRISIDYKGLLDSVKVGG 140

Query: 131 VILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDIL 190
            IL +DG IS S+ + +KE G V CR  N++ LGE KNV+LPG IV+LP ++EKD  DI 
Sbjct: 141 YILIADGVISLSITAVEKEKGHVVCRVNNNSRLGENKNVHLPGAIVNLPAVSEKDILDI- 200

Query: 191 EWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 250
           ++GV  N+D IA SF+RK  D+ E+R++LG+  K I ++SK+EN EGV NF++IL  SD 
Sbjct: 201 KFGVEQNVDFIAASFIRKADDVNEIREILGEKGKDIQIISKIENVEGVDNFNEILEVSDG 260

Query: 251 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 310
            MVARGDLG+E+ +EKIF+AQK+++ KCN  GKPV+TATQMLESMIK+PRPTRAEATDVA
Sbjct: 261 IMVARGDLGVEVQMEKIFVAQKMIVSKCNAAGKPVITATQMLESMIKNPRPTRAEATDVA 320

Query: 311 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEA---ESTLNYGDVFKRIMEHSPVPM 370
           NAVLDG+DCVMLSGETA+G YP  AV  MAKIC EA   ES+ +Y  +F  +   S  P+
Sbjct: 321 NAVLDGSDCVMLSGETASGDYPYEAVDIMAKICREAELVESSTDYQTLFAALKLSSAKPV 380

Query: 371 SPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWS 430
           S  E++AS AV TA   KA LI+ LT  G TA+LV+KYRP  PI++V           WS
Sbjct: 381 SIAETVASYAVATAIDLKADLIITLTETGLTARLVSKYRPSIPIIAVT---------SWS 440

Query: 431 CSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALH 490
            +     +H L  RG +P L  +         T++ +E  +++A    LCK G  VV + 
Sbjct: 441 YT----VKHLLATRGAIPFLVESLV------GTDKLVESCLEYAMKHNLCKKGSRVVIVS 498

Query: 491 RV-----GTASVIKILTV 500
            V     G  + +++LTV
Sbjct: 501 GVMEGVPGKTNSLRVLTV 498

BLAST of CmaCh02G011610 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 875.2 bits (2260), Expect = 2.6e-254
Identity = 443/496 (89.31%), Postives = 467/496 (94.15%), Query Frame = 0

Query: 5   VIEKKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMEN 64
           +IE++PKTKIVCTLGPASRSVPMVEKLL+AGMNVARFNFSHGSHEYHQETLDNL  AM N
Sbjct: 3   MIEQRPKTKIVCTLGPASRSVPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLHQAMLN 62

Query: 65  TGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAED 124
           TGILCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDY LKGDEN ICMSYKKLA D
Sbjct: 63  TGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKLAVD 122

Query: 125 VKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKD 184
           V PG VILC+DGTIS  VLSCDKE+G VRCRCENSA+LGERKNVNLPGV+VDLPTLTEKD
Sbjct: 123 VNPGMVILCADGTISLLVLSCDKENGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTEKD 182

Query: 185 KEDILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDIL 244
           KEDI++WGVPN IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFDDIL
Sbjct: 183 KEDIMQWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKNILLMSKVENQEGVANFDDIL 242

Query: 245 ANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAE 304
            NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAE
Sbjct: 243 VNSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAE 302

Query: 305 ATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPV 364
           ATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGDVFKRIM +SPV
Sbjct: 303 ATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMLYSPV 362

Query: 365 PMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFD 424
           PMSPLESLASSAVRTANSA+A LI+VLTRGGSTA+LVAKYRPG PILSVVVPEIKTD FD
Sbjct: 363 PMSPLESLASSAVRTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTDFFD 422

Query: 425 WSCSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVA 484
           WSCSDE+PARHSLIFRGL+PVL   SA++SH E+TEEAIEFA Q+ K K LCK GDSVVA
Sbjct: 423 WSCSDESPARHSLIFRGLIPVLYAGSARASHDESTEEAIEFATQYGKEKELCKTGDSVVA 482

Query: 485 LHRVGTASVIKILTVK 501
           L RVG ASVIKILTVK
Sbjct: 483 LLRVGNASVIKILTVK 498

BLAST of CmaCh02G011610 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 841.3 bits (2172), Expect = 4.1e-244
Identity = 422/497 (84.91%), Postives = 458/497 (92.15%), Query Frame = 0

Query: 4   LVIEKKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAME 63
           + +E++PKTKIVCTLGPASRSVPMVEKLL AGM+VARFNFSHGS+EYHQETLDNLR AM 
Sbjct: 1   MAMEQRPKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAML 60

Query: 64  NTGILCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAE 123
           NTG+LCAVMLDTKGPEIRTGFLKDGKPIQL QGQEITISTDY LKGDE  ICMSYKKLA+
Sbjct: 61  NTGMLCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQ 120

Query: 124 DVKPGSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEK 183
           DV PG VILC+DGTIS  VLSCDKE G VRCRCEN+++LGERKNVNLPGV+VDLPTLTEK
Sbjct: 121 DVNPGMVILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEK 180

Query: 184 DKEDILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDI 243
           DK+DILEWGVPN IDMIALSFVRKGSDLV+VRKLLGKHAK+ILLMSKVENQEGVANFDDI
Sbjct: 181 DKQDILEWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDI 240

Query: 244 LANSDAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRA 303
           L NSDAFM+ARGDLGMEIPIEKIFLAQKVMIYKCN  GKPVVTATQMLESMIKSPRPTRA
Sbjct: 241 LINSDAFMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRA 300

Query: 304 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSP 363
           EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTL+YGD+FKRIM H+ 
Sbjct: 301 EATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAA 360

Query: 364 VPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSF 423
           VPMSP+ESLASSAVRTA S++A L++VLTRGGSTA+LVAKYRPG PILSVVVPEI +DSF
Sbjct: 361 VPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSF 420

Query: 424 DWSCSDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVV 483
           DW+CS+EAPARHSLI+RGLVPVL   SA++S  E+TEE +EFA ++ K K LCK GDSVV
Sbjct: 421 DWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVV 480

Query: 484 ALHRVGTASVIKILTVK 501
           AL R G A VIKILTVK
Sbjct: 481 ALFRTGNAIVIKILTVK 497

BLAST of CmaCh02G011610 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 788.9 bits (2036), Expect = 2.4e-228
Identity = 391/491 (79.63%), Postives = 441/491 (89.82%), Query Frame = 0

Query: 10  PKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGILC 69
           PKTKIVCTLGPASR+V M+EKLLKAGMNVARFNFSHGSHEYHQETLDNLR+AM NTGIL 
Sbjct: 20  PKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRSAMHNTGILA 79

Query: 70  AVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGS 129
           AVMLDTKGPEIRTGFLKDG PIQL +GQEITI+TDY ++GDE+ I MSYKKL  DVKPG+
Sbjct: 80  AVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGDESTISMSYKKLPLDVKPGN 139

Query: 130 VILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDIL 189
            ILC+DG+IS +VLSCD ESG VRCRCENSA+LGERKNVNLPGV+VDLPTLT+KD EDIL
Sbjct: 140 TILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTDKDIEDIL 199

Query: 190 EWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDA 249
            WGVPN+IDMIALSFVRKGSDLV VRK+LG HAKSI+LMSKVENQEGV NFD+IL  +DA
Sbjct: 200 GWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMSKVENQEGVINFDEILRETDA 259

Query: 250 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 309
           FMVARGDLGMEIPIEKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 260 FMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319

Query: 310 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMSPL 369
           NAVLDGTDCVMLSGE+AAGAYPE+AV+ MAKIC+EAES+L+Y  +FK ++  +P+PMSPL
Sbjct: 320 NAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLDYNTIFKEMIRATPLPMSPL 379

Query: 370 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSCSD 429
           ESLASSAVRTAN A+A LI+VLTRGGSTA LVAKYRP  PILSVVVP + TDSFDWSCSD
Sbjct: 380 ESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSFDWSCSD 439

Query: 430 EAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALHRVG 489
           E+PARHSLI+RGL+P+L+  SAK++ +E TE  IE A++ A  +GLC  GD++VALHR+G
Sbjct: 440 ESPARHSLIYRGLIPMLAEGSAKATDSEATEVIIEAALKSATQRGLCNRGDAIVALHRIG 499

Query: 490 TASVIKILTVK 501
            ASVIKI  VK
Sbjct: 500 AASVIKICVVK 510

BLAST of CmaCh02G011610 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 787.3 bits (2032), Expect = 7.1e-228
Identity = 390/493 (79.11%), Postives = 442/493 (89.66%), Query Frame = 0

Query: 8   KKPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGI 67
           + PKTKIVCTLGPASRSV M+EKLLKAGMNVARFNFSHGSHEYHQETLDNLR AM+NTGI
Sbjct: 18  RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGI 77

Query: 68  LCAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKP 127
           L AVMLDTKGPEIRTGFLKDG PIQL +GQEITI+TDY +KGDE  I MSYKKL  DVKP
Sbjct: 78  LAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTISMSYKKLPVDVKP 137

Query: 128 GSVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKED 187
           G+ ILC+DG+IS +V+SCD  +G V CRCEN+A+LGERKNVNLPGV+VDLPTLT+KD ED
Sbjct: 138 GNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNLPGVVVDLPTLTDKDVED 197

Query: 188 ILEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANS 247
           IL+WGVPNNIDMIALSFVRKGSDLV VRK+LG H+KSI+LMSKVENQEGV NFD+IL  +
Sbjct: 198 ILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSKSIMLMSKVENQEGVLNFDEILRET 257

Query: 248 DAFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATD 307
           DAFMVARGDLGMEIPIEKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATD
Sbjct: 258 DAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATD 317

Query: 308 VANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMS 367
           VANAVLDGTDCVMLSGE+AAGAYPE+AV+TMAKIC+EAES+L+Y  +FK ++  +P+PMS
Sbjct: 318 VANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSLDYNTIFKEMIRATPLPMS 377

Query: 368 PLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEIKTDSFDWSC 427
            LESLASSAVRTAN AKA LI+VLTRGG+TAKLVAKYRP  PILSVVVP   +D+F+WSC
Sbjct: 378 TLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTFNWSC 437

Query: 428 SDEAPARHSLIFRGLVPVLSTASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVALHR 487
           SDE+PARHSLI+RGL+PVL   SAK++ +E+TEE IE A++ A  KGLC +GD+VVALHR
Sbjct: 438 SDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCNHGDAVVALHR 497

Query: 488 VGTASVIKILTVK 501
           +G ASVIKI  VK
Sbjct: 498 IGAASVIKICVVK 510

BLAST of CmaCh02G011610 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 738.0 bits (1904), Expect = 4.9e-213
Identity = 377/496 (76.01%), Postives = 432/496 (87.10%), Query Frame = 0

Query: 9   KPKTKIVCTLGPASRSVPMVEKLLKAGMNVARFNFSHGSHEYHQETLDNLRAAMENTGIL 68
           K KTKIVCTLGPASRSV MVEKLLKAGMNVARFNFSHGSH YHQETLDNLR AMENT I 
Sbjct: 15  KSKTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIP 74

Query: 69  CAVMLDTKGPEIRTGFLKDGKPIQLTQGQEITISTDYSLKGDENMICMSYKKLAEDVKPG 128
           CAVMLDTKGPEIRTGFLK+GKP++L QGQEITISTDY+++GD N I MSYKKLAED+K G
Sbjct: 75  CAVMLDTKGPEIRTGFLKEGKPVELIQGQEITISTDYTMEGDSNTISMSYKKLAEDLKSG 134

Query: 129 SVILCSDGTISFSVLSCDKESGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDI 188
            VILCSDGTIS +VLSCDK  GLVR RCENSAVLGERKNVNLPG++VDLPTLTEKD+EDI
Sbjct: 135 DVILCSDGTISLTVLSCDKNLGLVRARCENSAVLGERKNVNLPGIVVDLPTLTEKDQEDI 194

Query: 189 LEWGVPNNIDMIALSFVRKGSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSD 248
           L+WGVPN ID+IALSFVRKGSDLVEVRKLLG++AKSI+LMSKVENQEGV NFD IL  SD
Sbjct: 195 LQWGVPNKIDIIALSFVRKGSDLVEVRKLLGENAKSIMLMSKVENQEGVMNFDKILEYSD 254

Query: 249 AFMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDV 308
           AFMVARGDLGMEIPIEK+FLAQK+MI K N  GKP+VTATQMLESM KSPRPTRAEATDV
Sbjct: 255 AFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRPTRAEATDV 314

Query: 309 ANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLNYGDVFKRIMEHSPVPMSP 368
           ANAVLDGTDCVMLSGETAAGA+PE AV TM++IC EAE  ++Y  + K+I +   +P+SP
Sbjct: 315 ANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTMHKKIQDIVSLPLSP 374

Query: 369 LESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSVVVPEI-KTDSFDWSC 428
           +ESLA+SAV TA S  AA I+VLT+GG T +LVAKYRP  PILSV+VPEI +TD F+WSC
Sbjct: 375 IESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVPEITRTDDFEWSC 434

Query: 429 SDEAP--ARHSLIFRGLVPVLST-ASAKSSHAETTEEAIEFAIQHAKLKGLCKNGDSVVA 488
           S+ A   AR  LI+RG+VPV++T ASA+SS+ ++TEE I+FAI+ AK KG+CK GDS+VA
Sbjct: 435 SESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEFAKKKGICKTGDSIVA 494

Query: 489 LHRVGTASVIKILTVK 501
           LH++  +SV+KIL V+
Sbjct: 495 LHKIDGSSVVKILNVE 510