CmaCh02G011170 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G011170
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr02: 6634770 .. 6637841 (+)
RNA-Seq Expression	CmaCh02G011170
Synteny	CmaCh02G011170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACCAAGGAACAACCATTCCCCTTTATTTAAAGCTTATTCTTCTCCCATGCAAATCCACATTCAACCATGAACAACTACAACAAGCTCCACTCCTCCGGCTCCGGCGACACGCCGGCCACCTTCCAAGTTTCTCATACTCCCAATCCACCACATCCTCCGTCCTCCTCCGCCTCACGCCGCCGCACCCGAATCCTCTTTGTCTCCCTTATCTCGATCCTTCTGATCACCGCGTCCGCCGTCTCCGCCGCCGTCCTCATCCATTCCCACAGCTCCCATATCCCCCACAACAAACCCACCCAAGCCATCTCCCACGCCTGCAGCCGGACTCTCTACCCTTCTCTCTGCCGCTCTTCTCTCCTCTCCTTCCCCGGCTCTCTCTCCGCCGACGTCCACCACCTCGTCCATATCTCTCTCAACTTAACCCTCCAACACCTCACCAAAGCCCTCTACGCCTCTTCTCAAATCCCGGTTTTTCAAATCTCCAATAACCCTCTAGCTCACTCTGCTTACCAAGACTGTATCGAGCTTCTAAACGACGCCGTTGACGCCTTCTCTCTCTCTCTCTTCTCCACCTCAGCTTCTAACCATGACATCATGACGTGGCTTAGTGCTGCGTTGACTTATCATGACACATGTAGTGATGGATTTGCGGATGTGGATGACCCGGTCAAGGATCAAATGGCGTCGAGGCTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATTTTTTCTGGGTATGGTGAGGGGTTGCCGGTGCAGAATAGGCGGCGGCTGATGGAGAGTGATAGGGAGTGGGCGGCGGAGAATGGTGTCGCGGTGGCGGAGGGGGAGTTTCCCGGGTGGGTTAGTGGGAAGGATAGGCGGCTTTTGGCTGCTTCCATTTCGGCCATTCAAGCGGATATTGTTGTTGCAAAGGATGGGAGTGGGAAGTTCAAGACGGTGGCGGAGGCCATTGAGGCGGCACCGTCGTCTAGTGGTCGACGAATCGTTATCTACATTAAGGCTGGAAAGTAAGATCAAATTTCAAAAAATTTCAAAATTTATTTTAATGGAAATAATTTCTATTTTTATTAAAACATTCTAAATAATTAATTATATTTTATAAACAAATCAAAATACTTTTTTTTTTTTTTTTTATAAATTGTAGCTATTTTTATTTTTTTTAAAATAATAAAAATTTTGGGAATATTTTATTAAAATGACATTAAAAAAATTAATTTTAAAAAAATAGAAAACTATGAGGCCCTAAAGTTGGTGGGTGGAATTATTAGGCCATAATTAATAACTTAACCCTCATTAAAAGGAAGGGAAAATAAATTATTTTTAACAAATTTTATCCTATGTTTAATCATTGACTTCATGTTATTATTTATCATTTCATAACCTATAGTTTTCATAATAATATTTTACTGCATACGTAATAATCTTTCTGCATACTACAATTAAATAGTTAAAAGTCGGGATAAAGGGTATTAATGTAATTTTGCAGGTACGAAGAAGAGAATTTGAAGGTGGGAAGGAAGAAGACAAACTTGATGTTTGTGGGAGATGGGAAGGGTATAACCGTCATTTCAGGTGGCAAAAGCGTTTTTGACAAAGTTACTACTTTTCGCACCGCCACCTTTGGTATATACTCTCCATCTCTTTTTTCAATTTTATTCCAAAACTTTTTCTTTTTTTAATTCTTATGAATTAAATTTAAATAAATGAAAGAAAATAAAACTAGTGATGACGTGTCGTTAGTGCATTAGTGTGTTAATATAAATATATTAATTAAATGTTTTATTAGGATAAGCATTGAGTGAGTAAGCTATTTAAAGATAATGATAAATTAAGCTAATTTTTGAAGCATTTATTAGGCTACAAAATAATTAATTGATTTATCCACGTAATCATACGAGAAATTGTAAATTCCAGCTGTGTTTGCCAACTGGCAGGTAGCCTCCTTTCTTCTCTGCATTATTATTTTTTTAGGCCAAGTGGGCCCATTCATATTTATATGCTAATTGGTGCATTTAATTAGTAAAGTCAAAGTTCATACTATGTAATTTAATTTCATATAACTTAATGTTATAAATATTTTATATACGATAAATAAATTATTATTTATATTATTTTGCAGCCGGTTCTGGGACAGGAATAATTTTACGAGACATGACAATCGAGAACACTGCGGGGCCAAGCAAGCACCAAGCAGTGGCACTTCGTGTAAGTGCAGACCATGCGGTGGTGTATCGTTGCAACATAATTGGTTACCAAGACACTTTGTACGTGCATTCGAATCGACAGTTTTTTCGAGAGTGTGACATATATGGTACAGTGGACTTTATTTTCGGAAATGCAGTAGTGGTGTTTCAAAGCTGCAATATCTATGCTCGGAAGCCTATGCCCCAACAAAAGAACACTATCACTGCTCAAAACCGAAAGGATCCGAATCAGAATACTGGAATCTCCATCCATGCCTGCAAGATCCTCGCTACCACTGACCTTGAAGCCTCCAAAGGCTCTTTTTCGACTTTCCTCGGTCGCCCTTGGAAATTATACTCTAGAGTTGTTTACATGGTATCTTCAATGGGGGATCACATTCATCCACGTGGTTGGCTCGAGTGGCAAGGCTCTTTTGCTCTCGACACTCTTTATTACGGCGAGTATATGAATTCTGGGCCAGGAGCTGCCACAGGGCAGCGAGTCAACTGGCCAGGTTATCGGGTCATTACTTCTACGATTGAAGCCACCAAATTTACTGTCGGACAATTTATCTATGGCTCTTCGTGGTTGCCTTCCACTGGAGTGTCGTTTTCTGCTGGATTATCTGGTAACTAAAATTAAAATTGCAATACTATTTTTGTAGTATTAATTTTTTAGGATGTGGTTGATGACGAAACGTGTACAGTCTCATTCCTTTGATTAATGCTCTTGCCAGGAAAATGAAGAAAAGAGAAGAAAATAATGTGTGCCACATTCCAATTATGAGAGTTTATGTATTGTTATAATTGTGATTGTATTTGTTGTATCATCGTTATATATTTTTTTCAGAATAAATTTGTTTTCTTGTTTTATTTTTA

mRNA sequence

GACCAAGGAACAACCATTCCCCTTTATTTAAAGCTTATTCTTCTCCCATGCAAATCCACATTCAACCATGAACAACTACAACAAGCTCCACTCCTCCGGCTCCGGCGACACGCCGGCCACCTTCCAAGTTTCTCATACTCCCAATCCACCACATCCTCCGTCCTCCTCCGCCTCACGCCGCCGCACCCGAATCCTCTTTGTCTCCCTTATCTCGATCCTTCTGATCACCGCGTCCGCCGTCTCCGCCGCCGTCCTCATCCATTCCCACAGCTCCCATATCCCCCACAACAAACCCACCCAAGCCATCTCCCACGCCTGCAGCCGGACTCTCTACCCTTCTCTCTGCCGCTCTTCTCTCCTCTCCTTCCCCGGCTCTCTCTCCGCCGACGTCCACCACCTCGTCCATATCTCTCTCAACTTAACCCTCCAACACCTCACCAAAGCCCTCTACGCCTCTTCTCAAATCCCGGTTTTTCAAATCTCCAATAACCCTCTAGCTCACTCTGCTTACCAAGACTGTATCGAGCTTCTAAACGACGCCGTTGACGCCTTCTCTCTCTCTCTCTTCTCCACCTCAGCTTCTAACCATGACATCATGACGTGGCTTAGTGCTGCGTTGACTTATCATGACACATGTAGTGATGGATTTGCGGATGTGGATGACCCGGTCAAGGATCAAATGGCGTCGAGGCTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATTTTTTCTGGGTATGGTGAGGGGTTGCCGGTGCAGAATAGGCGGCGGCTGATGGAGAGTGATAGGGAGTGGGCGGCGGAGAATGGTGTCGCGGTGGCGGAGGGGGAGTTTCCCGGGTGGGTTAGTGGGAAGGATAGGCGGCTTTTGGCTGCTTCCATTTCGGCCATTCAAGCGGATATTGTTGTTGCAAAGGATGGGAGTGGGAAGTTCAAGACGGTGGCGGAGGCCATTGAGGCGGCACCGTCGTCTAGTGGTCGACGAATCGTTATCTACATTAAGGCTGGAAAGTACGAAGAAGAGAATTTGAAGGTGGGAAGGAAGAAGACAAACTTGATGTTTGTGGGAGATGGGAAGGGTATAACCGTCATTTCAGGTGGCAAAAGCGTTTTTGACAAAGTTACTACTTTTCGCACCGCCACCTTTGCCGGTTCTGGGACAGGAATAATTTTACGAGACATGACAATCGAGAACACTGCGGGGCCAAGCAAGCACCAAGCAGTGGCACTTCGTGTAAGTGCAGACCATGCGGTGGTGTATCGTTGCAACATAATTGGTTACCAAGACACTTTGTACGTGCATTCGAATCGACAGTTTTTTCGAGAGTGTGACATATATGGTACAGTGGACTTTATTTTCGGAAATGCAGTAGTGGTGTTTCAAAGCTGCAATATCTATGCTCGGAAGCCTATGCCCCAACAAAAGAACACTATCACTGCTCAAAACCGAAAGGATCCGAATCAGAATACTGGAATCTCCATCCATGCCTGCAAGATCCTCGCTACCACTGACCTTGAAGCCTCCAAAGGCTCTTTTTCGACTTTCCTCGGTCGCCCTTGGAAATTATACTCTAGAGTTGTTTACATGGTATCTTCAATGGGGGATCACATTCATCCACGTGGTTGGCTCGAGTGGCAAGGCTCTTTTGCTCTCGACACTCTTTATTACGGCGAGTATATGAATTCTGGGCCAGGAGCTGCCACAGGGCAGCGAGTCAACTGGCCAGGTTATCGGGTCATTACTTCTACGATTGAAGCCACCAAATTTACTGTCGGACAATTTATCTATGGCTCTTCGTGGTTGCCTTCCACTGGAGTGTCGTTTTCTGCTGGATTATCTGGTAACTAAAATTAAAATTGCAATACTATTTTTGTAGTATTAATTTTTTAGGATGTGGTTGATGACGAAACGTGTACAGTCTCATTCCTTTGATTAATGCTCTTGCCAGGAAAATGAAGAAAAGAGAAGAAAATAATGTGTGCCACATTCCAATTATGAGAGTTTATGTATTGTTATAATTGTGATTGTATTTGTTGTATCATCGTTATATATTTTTTTCAGAATAAATTTGTTTTCTTGTTTTATTTTTA

Coding sequence (CDS)

ATGAACAACTACAACAAGCTCCACTCCTCCGGCTCCGGCGACACGCCGGCCACCTTCCAAGTTTCTCATACTCCCAATCCACCACATCCTCCGTCCTCCTCCGCCTCACGCCGCCGCACCCGAATCCTCTTTGTCTCCCTTATCTCGATCCTTCTGATCACCGCGTCCGCCGTCTCCGCCGCCGTCCTCATCCATTCCCACAGCTCCCATATCCCCCACAACAAACCCACCCAAGCCATCTCCCACGCCTGCAGCCGGACTCTCTACCCTTCTCTCTGCCGCTCTTCTCTCCTCTCCTTCCCCGGCTCTCTCTCCGCCGACGTCCACCACCTCGTCCATATCTCTCTCAACTTAACCCTCCAACACCTCACCAAAGCCCTCTACGCCTCTTCTCAAATCCCGGTTTTTCAAATCTCCAATAACCCTCTAGCTCACTCTGCTTACCAAGACTGTATCGAGCTTCTAAACGACGCCGTTGACGCCTTCTCTCTCTCTCTCTTCTCCACCTCAGCTTCTAACCATGACATCATGACGTGGCTTAGTGCTGCGTTGACTTATCATGACACATGTAGTGATGGATTTGCGGATGTGGATGACCCGGTCAAGGATCAAATGGCGTCGAGGCTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATTTTTTCTGGGTATGGTGAGGGGTTGCCGGTGCAGAATAGGCGGCGGCTGATGGAGAGTGATAGGGAGTGGGCGGCGGAGAATGGTGTCGCGGTGGCGGAGGGGGAGTTTCCCGGGTGGGTTAGTGGGAAGGATAGGCGGCTTTTGGCTGCTTCCATTTCGGCCATTCAAGCGGATATTGTTGTTGCAAAGGATGGGAGTGGGAAGTTCAAGACGGTGGCGGAGGCCATTGAGGCGGCACCGTCGTCTAGTGGTCGACGAATCGTTATCTACATTAAGGCTGGAAAGTACGAAGAAGAGAATTTGAAGGTGGGAAGGAAGAAGACAAACTTGATGTTTGTGGGAGATGGGAAGGGTATAACCGTCATTTCAGGTGGCAAAAGCGTTTTTGACAAAGTTACTACTTTTCGCACCGCCACCTTTGCCGGTTCTGGGACAGGAATAATTTTACGAGACATGACAATCGAGAACACTGCGGGGCCAAGCAAGCACCAAGCAGTGGCACTTCGTGTAAGTGCAGACCATGCGGTGGTGTATCGTTGCAACATAATTGGTTACCAAGACACTTTGTACGTGCATTCGAATCGACAGTTTTTTCGAGAGTGTGACATATATGGTACAGTGGACTTTATTTTCGGAAATGCAGTAGTGGTGTTTCAAAGCTGCAATATCTATGCTCGGAAGCCTATGCCCCAACAAAAGAACACTATCACTGCTCAAAACCGAAAGGATCCGAATCAGAATACTGGAATCTCCATCCATGCCTGCAAGATCCTCGCTACCACTGACCTTGAAGCCTCCAAAGGCTCTTTTTCGACTTTCCTCGGTCGCCCTTGGAAATTATACTCTAGAGTTGTTTACATGGTATCTTCAATGGGGGATCACATTCATCCACGTGGTTGGCTCGAGTGGCAAGGCTCTTTTGCTCTCGACACTCTTTATTACGGCGAGTATATGAATTCTGGGCCAGGAGCTGCCACAGGGCAGCGAGTCAACTGGCCAGGTTATCGGGTCATTACTTCTACGATTGAAGCCACCAAATTTACTGTCGGACAATTTATCTATGGCTCTTCGTGGTTGCCTTCCACTGGAGTGTCGTTTTCTGCTGGATTATCTGGTAACTAA

Protein sequence

MNNYNKLHSSGSGDTPATFQVSHTPNPPHPPSSSASRRRTRILFVSLISILLITASAVSAAVLIHSHSSHIPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGLPVQNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGLSGN

Homology

[Close]

BLAST of CmaCh02G011170 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 3.2e-200
Identity = 366/586 (62.46%), Postives = 434/586 (74.06%), Query Frame = 0


Query: 25  PNPPHPPSSSAS-RRRTR---ILFVSLISILLITASAVSAAVLIHSHSSHIPH--NKPTQ 84
           PN   P +S    +++T+   ILF   + ++ +    + A +         P    KPTQ
Sbjct: 14  PNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQ 73

Query: 85  AISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQI 144
           AIS  CS++LYP+LC  +LL FPGSL+AD + L+HIS N TLQ  +KALY SS I   Q+
Sbjct: 74  AISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM 133

Query: 145 SNNPLAHSAYQDCIELLNDAVDAFSLSLFST-----SASNHDIMTWLSAALTYHDTCSDG 204
              P   SAY  C+ELL+D+VDA + +L S        S+ D+MTWLS+A+T HDTC+DG
Sbjct: 134 P--PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDG 193

Query: 205 FADVD---DPVKDQMASRLSDLSEMISNSLAIFSGYGE---GLPVQNRRRLMESDREWAA 264
           F +++     VKDQ+   + DLSEM+SN LAIF+G  +   G+PV N R+L+ ++     
Sbjct: 194 FDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEE---- 253

Query: 265 ENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGR 324
                    E P W+  +DR LL    SAIQADI V+KDGSG FKT+AEAI+ AP  S R
Sbjct: 254 -------TEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSR 313

Query: 325 RIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGT 384
           R VIY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFA +G 
Sbjct: 314 RFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGA 373

Query: 385 GIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYG 444
           G I+RDMT EN AGP+KHQAVALRV  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IYG
Sbjct: 374 GFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYG 433

Query: 445 TVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEA 504
           TVDFIFGNA V+ QSCNIYARKPM QQK TITAQNRKDPNQNTGISIHACK+LAT DLEA
Sbjct: 434 TVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEA 493

Query: 505 SKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAA 564
           SKGS+ T+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G+ 
Sbjct: 494 SKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSG 553

Query: 565 TGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGLS 594
            GQRV WPGY VITST+EA+KFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 554 IGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

[Close]

BLAST of CmaCh02G011170 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.3e-193
Identity = 359/593 (60.54%), Postives = 439/593 (74.03%), Query Frame = 0


Query: 16  PATFQVSHTPNPPHPPSSSASRRRTRILFVS--LISILLITASAVSAAV---LIHSHSSH 75
           P   QVS +  P      + +RR  + L VS  +++I LI A+A+ A V   L  + S  
Sbjct: 23  PILNQVSTSEQP-----ENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVP 82

Query: 76  IPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSA-DVHHLVHISLNLTLQHLTKALYA 135
               KP+QAIS AC  T +P LC  SL+ FPGSL+A     L+H+++N+TL H + ALY+
Sbjct: 83  GLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYS 142

Query: 136 SSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFS---LSLFSTSASNHDIMTWLSAALTY 195
           S+ +    +   P A SAY  C+ELL+D+VDA S    S+ S+SA   D+ TWLSAALT 
Sbjct: 143 SASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTN 202

Query: 196 HDTCSDGFADVDD-PVKDQMASRLSDLSEMISNSLAIFSGYGE-----GLPVQNRRRLME 255
           HDTC++GF  VDD  VKD M + L +LSE++SN LAIFS   +     G+P+QNRR L  
Sbjct: 203 HDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLL-- 262

Query: 256 SDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEA 315
                    GV   E +FP W+  K+R +L   +S IQADI+V+KDG+G  KT++EAI+ 
Sbjct: 263 ---------GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKK 322

Query: 316 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 375
           AP +S RRI+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA
Sbjct: 323 APQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTA 382

Query: 376 TFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFF 435
           +FA +G G I RD+T EN AGP+KHQAVALR+ ADHAV+YRCNIIGYQDTLYVHSNRQFF
Sbjct: 383 SFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFF 442

Query: 436 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKIL 495
           RECDIYGTVDFIFGNA VV Q+C+IYARKPM  QKNTITAQNRKDPNQNTGISIHA ++L
Sbjct: 443 RECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVL 502

Query: 496 ATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYM 555
           A +DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTLYYGEY+
Sbjct: 503 AASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYL 562

Query: 556 NSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGLS 594
           NSGPG+  GQRV+WPGYRVI ST EA +FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 563 NSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

[Close]

BLAST of CmaCh02G011170 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 441.8 bits (1135), Expect = 1.2e-122
Identity = 252/581 (43.37%), Postives = 349/581 (60.07%), Query Frame = 0


Query: 37  RRRTRILFVSLISILLITASAVSAAVLIHSHSSHIPHNKPTQAISHACSRTLYPSLCRSS 96
           + +  +L  + +++L + A A  +A    ++            +  +CS T YP LC S+
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 97  LLSFPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLN 156
           +++  G        ++  S+NLT+  +    +   ++   +    P   +A  DC+E ++
Sbjct: 76  VVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETID 135

Query: 157 DAVDAF-----SLSLFSTSAS--NH--DIMTWLSAALTYHDTCSDGFA--DVDDPVKDQM 216
           + +D        L L+ T  +   H  D+ T +S+A+T  +TC DGF+  D D  V+  +
Sbjct: 136 ETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 195

Query: 217 ASRLSDLSEMISNSLAIFSGYGE---------GLPVQNRRRLMESDREWAAENGVAVA-- 276
                 +  M SN+LA+     +              N R+L E ++E      +A A  
Sbjct: 196 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 255

Query: 277 ---EGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVI 336
              EG +P W+S  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S +R VI
Sbjct: 256 LDSEG-WPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVI 315

Query: 337 YIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIIL 396
           +IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  G   + 
Sbjct: 316 HIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLA 375

Query: 397 RDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDF 456
           RD+T +NTAGPSKHQAVALRV +D +  Y C+++ YQDTLYVHSNRQFF +C I GTVDF
Sbjct: 376 RDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDF 435

Query: 457 IFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGS 516
           IFGNA VV Q C+I+AR+P   QKN +TAQ R DPNQNTGI I  C+I AT+DL++ KGS
Sbjct: 436 IFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGS 495

Query: 517 FSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQR 576
           F T+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G GA T  R
Sbjct: 496 FPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANR 555

Query: 577 VNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 593
           V W G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 556 VKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of CmaCh02G011170 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.7e-122
Identity = 250/579 (43.18%), Postives = 357/579 (61.66%), Query Frame = 0


Query: 38  RRTRILFVSLISILLITASAVSAAVLIHSHSSHIPHNKPTQAIS--------HACSRTLY 97
           +R + +++++++ +L+ A+ +     + SHS +   +    AIS         ACS TL+
Sbjct: 16  KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 75

Query: 98  PSLCRSSLLS---FPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHS 157
           P LC S++++   F   +++    ++ +SLN+T++ + +  YA  ++   +    P    
Sbjct: 76  PELCYSAIVNVSDFSKKVTSQ-KDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKV 135

Query: 158 AYQDCIELLNDAVDAF-----SLSLFSTSAS----NHDIMTWLSAALTYHDTCSDGFA-- 217
           A  DC+E +++ +D        L L+    S      D+ T +S+A+T  +TC DGF+  
Sbjct: 136 ALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHD 195

Query: 218 DVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGLPVQNRRRLM--ESDREWAAENGVAV 277
           + D  V+  +      + +M SN+LA+     +   + N  +L    ++R+   +N    
Sbjct: 196 EADKKVRKVLLKGQKHVEKMCSNALAMICNMTD-TDIANEMKLSAPANNRKLVEDN---- 255

Query: 278 AEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYI 337
             GE+P W+S  DRRLL +  S +  D+VVA DGSG +KTV+EA+  AP  S +R VI I
Sbjct: 256 --GEWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRI 315

Query: 338 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRD 397
           KAG Y  EN+ V +KKTN+MF+GDGK  T+I+  ++V D  TTF +AT       ++ RD
Sbjct: 316 KAGVY-RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARD 375

Query: 398 MTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIF 457
           +T +NTAG SKHQAVAL V +D +  YRC+++ YQDTLYVHSNRQFF +C + GTVDFIF
Sbjct: 376 ITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIF 435

Query: 458 GNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGSFS 517
           GN   VFQ C+I+AR+P   QKN +TAQ R DPNQNTGI I  C+I AT+DL   + SF 
Sbjct: 436 GNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFP 495

Query: 518 TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQRVN 577
           T+LGRPWK YSR V M SS+ D I P GW EW G+FALDTL+YGEY N+G GA T  RV 
Sbjct: 496 TYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVK 555

Query: 578 WPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 593
           W G++VITS+ EA  +T G+FI G SWL STG  FS GL
Sbjct: 556 WKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

[Close]

BLAST of CmaCh02G011170 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.9e-122
Identity = 255/574 (44.43%), Postives = 355/574 (61.85%), Query Frame = 0


Query: 38  RRTRILFVSLISILLITASAVSAAVLIHSH-SSHIPHNKPTQAI-SHACSRTLYPSLCRS 97
           ++ + LF++L + LL+ A+ +     ++S  +S    N+P  AI   +CS T YP LC S
Sbjct: 25  KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFS 84

Query: 98  SLLSFPGSLS--ADVHHLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIE 157
           ++ + P +         ++ +SLN+T   +    +   ++ + + +       A  DC+E
Sbjct: 85  AIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHDCLE 144

Query: 158 LLNDAVDAFSLSL-------FSTSASNH--DIMTWLSAALTYHDTCSDGFA--DVDDPVK 217
            +++ +D    ++          S S H  D+ T +SAA+T   TC DGF+  D +  V+
Sbjct: 145 TIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVR 204

Query: 218 DQMASRLSDLSEMISNSLAIFSGYGEG----LPVQNRRRLMESDREWAAENGVAVAEGEF 277
           D ++     + +M SN+LA+     +     +   N R+L+E           +  +G +
Sbjct: 205 DALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEE---------TSTVDG-W 264

Query: 278 PGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIKAGKY 337
           P W+S  DRRLL +  S++  ++VVA DGSG FKTVA ++ AAP    +R +I IKAG Y
Sbjct: 265 PAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVY 324

Query: 338 EEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDMTIEN 397
             EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT A  G G + RD+T +N
Sbjct: 325 -RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQN 384

Query: 398 TAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVV 457
           TAGPSKHQAVALRV AD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIFGNA  
Sbjct: 385 TAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAA 444

Query: 458 VFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGSFSTFLGR 517
           V Q+C+I+ARKP   QKN +TAQ R DPNQNTGI I   +I AT+DL+  +GSF T+LGR
Sbjct: 445 VLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGR 504

Query: 518 PWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQRVNWPGYR 577
           PWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N+G GA T  RV W G+R
Sbjct: 505 PWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFR 564

Query: 578 VITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 593
           VITS  EA  FT G FI GSSWL STG  FS GL
Sbjct: 565 VITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of CmaCh02G011170 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 699.5 bits (1804), Expect = 2.3e-201
Identity = 366/586 (62.46%), Postives = 434/586 (74.06%), Query Frame = 0


Query: 25  PNPPHPPSSSAS-RRRTR---ILFVSLISILLITASAVSAAVLIHSHSSHIPH--NKPTQ 84
           PN   P +S    +++T+   ILF   + ++ +    + A +         P    KPTQ
Sbjct: 14  PNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQ 73

Query: 85  AISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQI 144
           AIS  CS++LYP+LC  +LL FPGSL+AD + L+HIS N TLQ  +KALY SS I   Q+
Sbjct: 74  AISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM 133

Query: 145 SNNPLAHSAYQDCIELLNDAVDAFSLSLFST-----SASNHDIMTWLSAALTYHDTCSDG 204
              P   SAY  C+ELL+D+VDA + +L S        S+ D+MTWLS+A+T HDTC+DG
Sbjct: 134 P--PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDG 193

Query: 205 FADVD---DPVKDQMASRLSDLSEMISNSLAIFSGYGE---GLPVQNRRRLMESDREWAA 264
           F +++     VKDQ+   + DLSEM+SN LAIF+G  +   G+PV N R+L+ ++     
Sbjct: 194 FDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEE---- 253

Query: 265 ENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGR 324
                    E P W+  +DR LL    SAIQADI V+KDGSG FKT+AEAI+ AP  S R
Sbjct: 254 -------TEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSR 313

Query: 325 RIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGT 384
           R VIY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFA +G 
Sbjct: 314 RFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGA 373

Query: 385 GIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYG 444
           G I+RDMT EN AGP+KHQAVALRV  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IYG
Sbjct: 374 GFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYG 433

Query: 445 TVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEA 504
           TVDFIFGNA V+ QSCNIYARKPM QQK TITAQNRKDPNQNTGISIHACK+LAT DLEA
Sbjct: 434 TVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEA 493

Query: 505 SKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAA 564
           SKGS+ T+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G+ 
Sbjct: 494 SKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSG 553

Query: 565 TGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGLS 594
            GQRV WPGY VITST+EA+KFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 554 IGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

[Close]

BLAST of CmaCh02G011170 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 676.8 bits (1745), Expect = 1.6e-194
Identity = 359/593 (60.54%), Postives = 439/593 (74.03%), Query Frame = 0


Query: 16  PATFQVSHTPNPPHPPSSSASRRRTRILFVS--LISILLITASAVSAAV---LIHSHSSH 75
           P   QVS +  P      + +RR  + L VS  +++I LI A+A+ A V   L  + S  
Sbjct: 23  PILNQVSTSEQP-----ENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVP 82

Query: 76  IPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSA-DVHHLVHISLNLTLQHLTKALYA 135
               KP+QAIS AC  T +P LC  SL+ FPGSL+A     L+H+++N+TL H + ALY+
Sbjct: 83  GLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYS 142

Query: 136 SSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFS---LSLFSTSASNHDIMTWLSAALTY 195
           S+ +    +   P A SAY  C+ELL+D+VDA S    S+ S+SA   D+ TWLSAALT 
Sbjct: 143 SASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTN 202

Query: 196 HDTCSDGFADVDD-PVKDQMASRLSDLSEMISNSLAIFSGYGE-----GLPVQNRRRLME 255
           HDTC++GF  VDD  VKD M + L +LSE++SN LAIFS   +     G+P+QNRR L  
Sbjct: 203 HDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLL-- 262

Query: 256 SDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEA 315
                    GV   E +FP W+  K+R +L   +S IQADI+V+KDG+G  KT++EAI+ 
Sbjct: 263 ---------GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKK 322

Query: 316 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 375
           AP +S RRI+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA
Sbjct: 323 APQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTA 382

Query: 376 TFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFF 435
           +FA +G G I RD+T EN AGP+KHQAVALR+ ADHAV+YRCNIIGYQDTLYVHSNRQFF
Sbjct: 383 SFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFF 442

Query: 436 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKIL 495
           RECDIYGTVDFIFGNA VV Q+C+IYARKPM  QKNTITAQNRKDPNQNTGISIHA ++L
Sbjct: 443 RECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVL 502

Query: 496 ATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYM 555
           A +DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTLYYGEY+
Sbjct: 503 AASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYL 562

Query: 556 NSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGLS 594
           NSGPG+  GQRV+WPGYRVI ST EA +FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 563 NSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

[Close]

BLAST of CmaCh02G011170 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 441.8 bits (1135), Expect = 8.7e-124
Identity = 252/581 (43.37%), Postives = 349/581 (60.07%), Query Frame = 0


Query: 37  RRRTRILFVSLISILLITASAVSAAVLIHSHSSHIPHNKPTQAISHACSRTLYPSLCRSS 96
           + +  +L  + +++L + A A  +A    ++            +  +CS T YP LC S+
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 97  LLSFPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLN 156
           +++  G        ++  S+NLT+  +    +   ++   +    P   +A  DC+E ++
Sbjct: 76  VVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETID 135

Query: 157 DAVDAF-----SLSLFSTSAS--NH--DIMTWLSAALTYHDTCSDGFA--DVDDPVKDQM 216
           + +D        L L+ T  +   H  D+ T +S+A+T  +TC DGF+  D D  V+  +
Sbjct: 136 ETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 195

Query: 217 ASRLSDLSEMISNSLAIFSGYGE---------GLPVQNRRRLMESDREWAAENGVAVA-- 276
                 +  M SN+LA+     +              N R+L E ++E      +A A  
Sbjct: 196 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 255

Query: 277 ---EGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVI 336
              EG +P W+S  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S +R VI
Sbjct: 256 LDSEG-WPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVI 315

Query: 337 YIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIIL 396
           +IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  G   + 
Sbjct: 316 HIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLA 375

Query: 397 RDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDF 456
           RD+T +NTAGPSKHQAVALRV +D +  Y C+++ YQDTLYVHSNRQFF +C I GTVDF
Sbjct: 376 RDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDF 435

Query: 457 IFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGS 516
           IFGNA VV Q C+I+AR+P   QKN +TAQ R DPNQNTGI I  C+I AT+DL++ KGS
Sbjct: 436 IFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGS 495

Query: 517 FSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQR 576
           F T+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G GA T  R
Sbjct: 496 FPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANR 555

Query: 577 VNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 593
           V W G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 556 VKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of CmaCh02G011170 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 437.2 bits (1123), Expect = 2.1e-122
Identity = 258/578 (44.64%), Postives = 340/578 (58.82%), Query Frame = 0


Query: 37  RRRTRILFVSLISILLITASAVSAAVLIHSHSSHIPHNKPTQAISHA-----CSRTLYPS 96
           +   +++  S    LL+ AS V  A    + + +        + SHA     CS TLYP 
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKN-QKITTLSSTSHAILKSVCSSTLYPE 74

Query: 97  LCRSSLLSFPGSLSADVHHLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDC 156
           LC S++ +  G        ++  SLNLT + +    +A  ++   +    P   +A  DC
Sbjct: 75  LCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 134

Query: 157 IELLNDAVDAFSLSL-------FSTSASNH--DIMTWLSAALTYHDTCSDGFA--DVDDP 216
           +E +++ +D   +++          S   H  D+ T +S+A+T   TC DGF+  D D  
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 217 VKDQMASRLSDLSEMISNSLAIFSGYGE------GLPVQNRRRLMESDREWAAENGVAVA 276
           V+  +      +  M SN+LA+     E       L  ++      ++R+     G   +
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 254

Query: 277 EGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIK 336
           +G +P W+S  DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R VI+IK
Sbjct: 255 DG-WPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIK 314

Query: 337 AGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDM 396
           AG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + RD+
Sbjct: 315 AGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 374

Query: 397 TIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 456
           T +NTAGPSKHQAVALRV +D +  Y+C++  YQDTLYVHSNRQFF +C I GTVDFIFG
Sbjct: 375 TFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFG 434

Query: 457 NAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGSFST 516
           NA  V Q C+I AR+P   QKN +TAQ R DPNQNTGI I  C+I  T+DL A KG+F T
Sbjct: 435 NAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPT 494

Query: 517 FLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQRVNW 576
           +LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA T  RV W
Sbjct: 495 YLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKW 554

Query: 577 PGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 593
            GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 555 KGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]

BLAST of CmaCh02G011170 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 430.6 bits (1106), Expect = 2.0e-120
Identity = 227/457 (49.67%), Postives = 299/457 (65.43%), Query Frame = 0


Query: 146 SAYQDCIELLNDAVDAFSLSLFSTSASN----------HDIMTWLSAALTYHDTCSDGFA 205
           +A  DC++LL+ A +  S  + ++ + N           D+ TW+SAAL+  DTC DGF 
Sbjct: 79  NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138

Query: 206 DVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGLPVQNRRRLMESDREWAAENGVAVAE 265
             +  +K  +A  LS +   + N L +        P + + + +++     A +G +   
Sbjct: 139 GTNGIIKKIVAGGLSKVGTTVRNLLTMV----HSPPSKPKPKPIKAQTMTKAHSGFS--- 198

Query: 266 GEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIVIYIKA 325
            +FP WV   DR+LL      + AD VVA DG+G F T+++A+ AAP  S +R VI++K 
Sbjct: 199 -KFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKR 258

Query: 326 GKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDMT 385
           G Y  EN+++ +KK N+M VGDG   TVI+G +S  D  TTFR+ATFA SG G I RD+T
Sbjct: 259 GVY-VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDIT 318

Query: 386 IENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 445
            +NTAGP KHQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GTVDFIFG+
Sbjct: 319 FQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGD 378

Query: 446 AVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGISIHACKILATTDLEASKGSFSTF 505
           A  VFQSC I A++ +P QKN+ITAQ RKDPN+ TG +I    I A TDL  +  + +T+
Sbjct: 379 ATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATY 438

Query: 506 LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAATGQRVNWP 565
           LGRPWKLYSR V+M + M D I+P GWLEW G+FALDTLYYGEYMNSGPGA+  +RV WP
Sbjct: 439 LGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWP 498

Query: 566 GYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 593
           GY V+ ++ EA  FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 GYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:

BLAST of CmaCh02G011170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FK05	3.2e-200	62.46	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B0	2.3e-193	60.54	Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3...	[more]
O49006	1.2e-122	43.37	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME...	[more]
Q43143	2.7e-122	43.18	Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM...	[more]
P83948	7.9e-122	44.43	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]

BLAST of CmaCh02G011170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53370.1	2.3e-201	62.46	pectin methylesterase PCR fragment F	[more]
AT3G49220.1	1.6e-194	60.54	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G14310.1	8.7e-124	43.37	pectin methylesterase 3	[more]
AT1G53830.1	2.1e-122	44.64	pectin methylesterase 2	[more]
AT3G43270.1	2.0e-120	49.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 75..222 e-value: 2.3E-40 score: 150.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 60..224 e-value: 9.0E-35 score: 117.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 79..222 e-value: 5.1E-32 score: 111.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 273..592 e-value: 2.9E-121 score: 406.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 77..225 e-value: 5.5E-36 score: 125.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 78..224
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 280..577 e-value: 4.9E-145 score: 481.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..37
None	No IPR available	PANTHER	PTHR31707:SF184	PECTINESTERASE	coord: 38..592
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 38..592
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 83..226 e-value: 2.4185E-42 score: 147.585
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 425..434
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 274..591

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G011170.1	CmaCh02G011170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity