CmaCh02G009870 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G009870
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	4a-hydroxytetrahydrobiopterin dehydratase
Location	Cma_Chr02: 5870295 .. 5871430 (-)
RNA-Seq Expression	CmaCh02G009870
Synteny	CmaCh02G009870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAAAAGGAACCCAAAGGCCCAGAGCCATTGGTGGTCGCTTCTGGAACTGTGTCCTTATCCATCAACCATGGCCACAACCCCAACCGCCAACTTCCCTCTCCTCCGTCTCCCCCGTCCCCACTCCCGTTTCTCTCTCCCACCGTCACCATCCACCACCCTCCTCCGCCGCAAACTCCGTCCGGCTACAATCAGAGCCGCCGTCGTAGGCGACGGCGACTTCGGCGCCAGAGACCCGTTCCCGGCGGAAATCGAGAGCAACTTTGGCGATAAGGTGATTGGATTTTCCAATACAGAGCACAAGATTCTAATCCCCAATGTCCGAGCCCTAGCTCTTTCTCAGCAACAGTGCGTTCCAATTTCATCCCTGCAGGCTCCAATGCCCGAAGATGAAGCCCAAAAACTCTTGAAGAAGGTAATCATATCTGATTTTTTGATTGTCCGATTGTTCGATTGTTCGATTGTTGATGAAATTTCATATAGGTCGTGGGGTGGAGATTGGTGGATAATGGAAGAGGCCAATTCAAACTCCGATGCTTGTGGAAATTGAGGGATTTCAAATGCTGTGTGGAGTTGATTAATAGAATCTCCAACGCTGTTGATGACGCTAACCATTTTCCAGATATTCACTTGGATCAGCCGAATCAAGTTACGGCAGAGCTCTGGACTTCAGCCATCGGTACTCATTCTTCTCCCTCTTTCAACCACCATCTTATATTAACTTGATTCATTGAGTTTTCATACAGAAATCTATGAGTTTTAAGGTCAGTAATTTTGGGATTTTGAATTTGCAGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCTAAAATTGATGAGATAAAAACCATAGATCTTGCTGCCAGGAAAAGAGCTTGGGCATAGCTGAATCTCATCTTCATTTTCTTGACAGAAAAATTTTCCCAGGAAAGTTTTCTTCTGAATTTTTGTTTTTGTTAAGGCCTCTTGTATATGAACTGTTTGATGATTTGATTTCTTGGGGCTGCTTGATAAGCAATTAGGATTGGAATTTGATCATTTTAATTTATTTCTAAGAATATAATTACTTTTTAAATGGGGTTTAAGAATTTGAAATACTAATACACTATTTTTTTTTTCTTTAATGGAAAAACATA

mRNA sequence

AATAAAAGGAACCCAAAGGCCCAGAGCCATTGGTGGTCGCTTCTGGAACTGTGTCCTTATCCATCAACCATGGCCACAACCCCAACCGCCAACTTCCCTCTCCTCCGTCTCCCCCGTCCCCACTCCCGTTTCTCTCTCCCACCGTCACCATCCACCACCCTCCTCCGCCGCAAACTCCGTCCGGCTACAATCAGAGCCGCCGTCGTAGGCGACGGCGACTTCGGCGCCAGAGACCCGTTCCCGGCGGAAATCGAGAGCAACTTTGGCGATAAGGTGATTGGATTTTCCAATACAGAGCACAAGATTCTAATCCCCAATGTCCGAGCCCTAGCTCTTTCTCAGCAACAGTGCGTTCCAATTTCATCCCTGCAGGCTCCAATGCCCGAAGATGAAGCCCAAAAACTCTTGAAGAAGGTCGTGGGGTGGAGATTGGTGGATAATGGAAGAGGCCAATTCAAACTCCGATGCTTGTGGAAATTGAGGGATTTCAAATGCTGTGTGGAGTTGATTAATAGAATCTCCAACGCTGTTGATGACGCTAACCATTTTCCAGATATTCACTTGGATCAGCCGAATCAAGTTACGGCAGAGCTCTGGACTTCAGCCATCGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCTAAAATTGATGAGATAAAAACCATAGATCTTGCTGCCAGGAAAAGAGCTTGGGCATAGCTGAATCTCATCTTCATTTTCTTGACAGAAAAATTTTCCCAGGAAAGTTTTCTTCTGAATTTTTGTTTTTGTTAAGGCCTCTTGTATATGAACTGTTTGATGATTTGATTTCTTGGGGCTGCTTGATAAGCAATTAGGATTGGAATTTGATCATTTTAATTTATTTCTAAGAATATAATTACTTTTTAAATGGGGTTTAAGAATTTGAAATACTAATACACTATTTTTTTTTTCTTTAATGGAAAAACATA

Coding sequence (CDS)

ATGGCCACAACCCCAACCGCCAACTTCCCTCTCCTCCGTCTCCCCCGTCCCCACTCCCGTTTCTCTCTCCCACCGTCACCATCCACCACCCTCCTCCGCCGCAAACTCCGTCCGGCTACAATCAGAGCCGCCGTCGTAGGCGACGGCGACTTCGGCGCCAGAGACCCGTTCCCGGCGGAAATCGAGAGCAACTTTGGCGATAAGGTGATTGGATTTTCCAATACAGAGCACAAGATTCTAATCCCCAATGTCCGAGCCCTAGCTCTTTCTCAGCAACAGTGCGTTCCAATTTCATCCCTGCAGGCTCCAATGCCCGAAGATGAAGCCCAAAAACTCTTGAAGAAGGTCGTGGGGTGGAGATTGGTGGATAATGGAAGAGGCCAATTCAAACTCCGATGCTTGTGGAAATTGAGGGATTTCAAATGCTGTGTGGAGTTGATTAATAGAATCTCCAACGCTGTTGATGACGCTAACCATTTTCCAGATATTCACTTGGATCAGCCGAATCAAGTTACGGCAGAGCTCTGGACTTCAGCCATCGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCTAAAATTGATGAGATAAAAACCATAGATCTTGCTGCCAGGAAAAGAGCTTGGGCATAG

Protein sequence

MATTPTANFPLLRLPRPHSRFSLPPSPSTTLLRRKLRPATIRAAVVGDGDFGARDPFPAEIESNFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEIKTIDLAARKRAWA

Homology

[Close]

BLAST of CmaCh02G009870 vs. ExPASy Swiss-Prot
Match: Q9LU63 (Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATP1 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 6.3e-58
Identity = 111/187 (59.36%), Postives = 138/187 (73.80%), Query Frame = 0


Query: 27  PSTTLLRRKLRPATI-RAAVVGD--GDFGARDPFPAEIESNFGDKVIGFSNTEHKILIPN 86
           P  +   RKL   T+ R+ +  D  GDFGARDP+P EI S FGDKV+G  +TEHKILIPN
Sbjct: 34  PPVSRNNRKLGRLTVTRSNLAQDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPN 93

Query: 87  VRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCC 146
              L+LSQ QC P+SS Q P+  D+A+ LL KV+GW +VDN  G  K+RC+WK+RDF C 
Sbjct: 94  ASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCG 153

Query: 147 VELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEIKTIDLA 206
           VELINRI    + + H+P +HL+ P QV AEL+TS+IGGLSMNDFI+AAKID+IKT DL+
Sbjct: 154 VELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 213

Query: 207 ARKRAWA 211
            RKRAWA
Sbjct: 214 PRKRAWA 220

[Close]

BLAST of CmaCh02G009870 vs. ExPASy Swiss-Prot
Match: Q6QJ72 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDL2 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.6e-19
Identity = 49/120 (40.83%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 89  LSQQQCVPISSLQ-APMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCCVELI 148
           LS ++CVP ++     M E  AQ LL+KV GW L  N     KL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLA-NDNDTLKLHRSWRVKSFTKGLDFF 125

Query: 149 NRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEIKTIDLAARKR 208
            R+++  +   H PD+HL   N V  E+WT AIGGL+ NDFI+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 184

[Close]

BLAST of CmaCh02G009870 vs. ExPASy Swiss-Prot
Match: Q8KFI4 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=CT0342 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-12
Identity = 37/109 (33.94%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 89  LSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCCVELIN 148
           L  + CVP     APM  +E Q+ L  +  W LVD+  G  KL  ++  +DF+  ++  N
Sbjct: 4   LENKHCVPCEGTAAPMASEELQRQLSSLPEWTLVDDS-GTSKLVRVFTFKDFQSALDFTN 63

Query: 149 RISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEI 198
           R+    +   H P + L +  +VT   WT AIGG+ +ND I+A K +++
Sbjct: 64  RVGQLAEAEGHHPAL-LTEWGKVTVSWWTHAIGGIHLNDVIMATKTEKL 110

[Close]

BLAST of CmaCh02G009870 vs. ExPASy Swiss-Prot
Match: B3QQ83 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_1693 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.6e-11
Identity = 35/109 (32.11%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 89  LSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCCVELIN 148
           L ++ CVP    +APM  +E Q+ L  +  W +VD+  G  KL  ++  +DF   ++   
Sbjct: 4   LEKKHCVPCEGTEAPMAPEELQRQLSHIPDWNVVDDS-GTPKLVRVFTFKDFNDALDFTV 63

Query: 149 RISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEI 198
           R+    +   H P + L +  +VT   WT AIGG+ +ND I+A K +++
Sbjct: 64  RVGQLAESEGHHPAL-LTEWGKVTVSWWTHAIGGIHLNDVIMATKTEKL 110

[Close]

BLAST of CmaCh02G009870 vs. ExPASy Swiss-Prot
Match: B0JQV0 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=MAE_39970 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.6e-11
Identity = 37/109 (33.94%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 89  LSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCCVELIN 148
           L+QQ+C P  S  +P+  +E   L  K+  W L++   G  +L+ L+K  +F+  +   N
Sbjct: 4   LAQQKCQPYQSGSSPITAEEITALQAKIPDWNLLEY-EGIPRLQKLYKFANFQGAIAFTN 63

Query: 149 RISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEI 198
            +  A +   H P + L +  +VT   WT  +GGL  NDFI+AA+ D+I
Sbjct: 64  AVGEAAEKEGHHPAL-LTEWGKVTVSWWTHDVGGLHQNDFIMAARTDDI 110

[Close]

BLAST of CmaCh02G009870 vs. TAIR 10
Match: AT5G51110.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-59
Identity = 111/187 (59.36%), Postives = 138/187 (73.80%), Query Frame = 0


Query: 27  PSTTLLRRKLRPATI-RAAVVGD--GDFGARDPFPAEIESNFGDKVIGFSNTEHKILIPN 86
           P  +   RKL   T+ R+ +  D  GDFGARDP+P EI S FGDKV+G  +TEHKILIPN
Sbjct: 34  PPVSRNNRKLGRLTVTRSNLAQDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPN 93

Query: 87  VRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCC 146
              L+LSQ QC P+SS Q P+  D+A+ LL KV+GW +VDN  G  K+RC+WK+RDF C 
Sbjct: 94  ASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCG 153

Query: 147 VELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEIKTIDLA 206
           VELINRI    + + H+P +HL+ P QV AEL+TS+IGGLSMNDFI+AAKID+IKT DL+
Sbjct: 154 VELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 213

Query: 207 ARKRAWA 211
            RKRAWA
Sbjct: 214 PRKRAWA 220

[Close]

BLAST of CmaCh02G009870 vs. TAIR 10
Match: AT5G51110.2 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-59
Identity = 114/194 (58.76%), Postives = 142/194 (73.20%), Query Frame = 0


Query: 22  SLPP---SPSTTLLRRKLRPATIRAAVVGD--GDFGARDPFPAEIESNFGDKVIGFSNTE 81
           S PP   S S+ LLR+  R      +++ D  GDFGARDP+P EI S FGDKV+G  +TE
Sbjct: 6   SSPPCNISASSLLLRQPSR------SILKDFLGDFGARDPYPEEIASQFGDKVLGCQSTE 65

Query: 82  HKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWK 141
           HKILIPN   L+LSQ QC P+SS Q P+  D+A+ LL KV+GW +VDN  G  K+RC+WK
Sbjct: 66  HKILIPNASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWK 125

Query: 142 LRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDE 201
           +RDF C VELINRI    + + H+P +HL+ P QV AEL+TS+IGGLSMNDFI+AAKID+
Sbjct: 126 VRDFGCGVELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDD 185

Query: 202 IKTIDLAARKRAWA 211
           IKT DL+ RKRAWA
Sbjct: 186 IKTSDLSPRKRAWA 193

[Close]

BLAST of CmaCh02G009870 vs. TAIR 10
Match: AT1G29810.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 95.5 bits (236), Expect = 5.4e-20
Identity = 49/120 (40.83%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 89  LSQQQCVPISSLQ-APMPEDEAQKLLKKVVGWRLVDNGRGQFKLRCLWKLRDFKCCVELI 148
           LS ++CVP ++     M E  AQ LL+KV GW L  N     KL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLA-NDNDTLKLHRSWRVKSFTKGLDFF 125

Query: 149 NRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMNDFIVAAKIDEIKTIDLAARKR 208
            R+++  +   H PD+HL   N V  E+WT AIGGL+ NDFI+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 184

The following BLAST results are available for this feature:

BLAST of CmaCh02G009870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU63	6.3e-58	59.36	Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis ...	[more]
Q6QJ72	7.6e-19	40.83	Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thalian...	[more]
Q8KFI4	2.4e-12	33.94	Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobaculum tepidum (stra...	[more]
B3QQ83	2.6e-11	32.11	Putative pterin-4-alpha-carbinolamine dehydratase OS=Chlorobaculum parvum (strai...	[more]
B0JQV0	2.6e-11	33.94	Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (str...	[more]

BLAST of CmaCh02G009870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51110.1	4.5e-59	59.36	Transcriptional coactivator/pterin dehydratase	[more]
AT5G51110.2	4.5e-59	58.76	Transcriptional coactivator/pterin dehydratase	[more]
AT1G29810.1	5.4e-20	40.83	Transcriptional coactivator/pterin dehydratase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	GENE3D	3.30.1360.20	Transcriptional coactivator/pterin dehydratase	coord: 104..202 e-value: 7.2E-22 score: 79.0
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	SUPERFAMILY	55248	PCD-like	coord: 102..197
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PFAM	PF01329	Pterin_4a	coord: 103..197 e-value: 6.7E-22 score: 77.4
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PANTHER	PTHR12599	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE	coord: 49..210
None	No IPR available	PANTHER	PTHR12599:SF8	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE, CHLOROPLASTIC-RELATED	coord: 49..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G009870.1	CmaCh02G009870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009651	response to salt stress
biological_process	GO:0110102	ribulose bisphosphate carboxylase complex assembly
biological_process	GO:0006729	tetrahydrobiopterin biosynthetic process
cellular_component	GO:0071944	cell periphery
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008124	4-alpha-hydroxytetrahydrobiopterin dehydratase activity