CmaCh02G004220 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh02G004220
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Location	Cma_Chr02: 2139252 .. 2143662 (-)
RNA-Seq Expression	CmaCh02G004220
Synteny	CmaCh02G004220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGTGATTATACTTTGAATTGGGGCAATACTTCAACAAGACTATTCTTGTCTTCACAGAACGACTGCAAAAGAGATCTCTGATCCCTCTTCGGACTCTGCCACTTCCTTAAACCCTAGCGCAATGTCGAAATCCCTCGTCGTCTTGATGCTAATCTCTGTTCTTCCATTTCTTTGCTATGCCTTCTCCCCCGAGAATCCCACAGATCGCAAAGTCCTCGTCTTACTCGATGATTTTGCCATCAAATCTACCCATTCTTTGTTCTTCAAGTCCCTCCAGTCCCGTGGTTTCGACCTCGATTTCAAGCTCGCCGATGACCCCAAGATCTCCTTGCAGAGACATGGACGCTATTTCTATGACGCATTGATCTTGTTTGCACCAACAATTGACCGTAAGCCTTCGTTCATTTCCATCTCTGATGGTTTCGTGTTGGAATTCTTGTATTTTTACTTGGAAATTCGAGACATAGTGTCAATTAGGGTTTCAGTTGTTTGGAATTTATTAATTTTGAGGTTTTATATTTTGACGTAGCCGGAATTACTCGTAATGAATTGCATATATGTTGTGATAATTGTTTACGTTTTAATTTCTCGGGTTTTGTAGGTTTTGGGGGATCCGTTGACCTTGCTGGTATTCTGGATTTTGTCGATTCGGGGCATGATCTAATTCTCGCTGCTGATTCTAATGCATCGGATTTGATCCGAGAAATTGCCACTGAATGTGGTGTTGATTTCGATGAGGTAGATGGATATCTAGGATATGTTACCTTTTTATCCATAGCAGTATGATATTTTTTCTTTATAGCGCAACAAGATTCTATGGAACCTTTTTTAAAAAGTTGATAATATTTAATTTCCATGAGCAAATGGATTATGTTTTATTTTTCGTATCAAAACTCCGTTTCCTTTCTGTACATTGTATTCTTATATGAGAAGTACTGTGTCAAAGGATTCAGCTGCTGTAGTCATTGACCACACCAGCTATGCAGTATCAGAGACAGAGGGAGATCACACATTGATTGCCAGTGACGATTTGATCAAGTCTGATGTAATTGTGGGAAGCAAGAAGATTGAGGCAAGTTAGATTCTTCAGTAATTTTAGAAAGATGATCATGTAGGAAGATAGTAGTTTTCATCCTTTATAATCAACTTTTTGTTGGGATTTTATTAATTATCGAAATTTCTTCAAAGTTTTCCTTGCCTTGGAGAAAATAGTCATTGATAATTCACATAATTAGTGTCTTAGTGTATGGCTGCTCATAATTTCTGAGAGTGTTGGCCATTCATCAGTGCATTTCTGTTTACTTTGGTCTTGATTTCTCCCATTTGGTACTAACTCTATTTTGTGTTTGTAACAGTCATTGTTACTAGAAGGCTTACTTCTAATTACTATCTGAATGAATGTTCCACACAGGCTCCTGTTCTTTTCCAAGGGATTGGCCATACCTTAAATCCTGTTAATAACTTGGTAAGATACTTGGATAGTCCTTACTTTACAACTTCTTCATAGAATGTGGTTCTATTAATGTCTTAATAAATCTGGATTGGCATGACCATTTTCATGTATTAACCTTCTCTTTTATTCTCCAGGTGCTAAAAGCTCTCTCAGCATCTCCCTCAGCATATTCTGCGAAGCCAAAGTCCAAGCTATCGAGCCCTCCGCAACTAAAAGGATCTGCTATCTCTCTGGTTTCAGTCATACAGGTCTATTATTGATCAAGTTCCTGCTTTTCTTTCTATGCATCTCTTTTAATGGTTTTTTTCTATGTCGCTGGAGCATAAAGTTTGATAAACAAAAAGATGGAGTATTGAATAGGCACAATAAAGTTCATTGTGTACCAGTTCACTTAGCTTTTATCTTGCTTCTGAAAGGACTTCTTGATTTACATACTTGGGTTTATTTCTTTAAACTGATGTTGTTCAAGTATGAAATTGAACTCTATTGGCTTTGTGTTCTAAGTTTTCCTCTGGTCTCCTAAAGCTGTAAGGTCTCTCATTTTTATCCATCTGGCAGGGAGCAACCTTTTAAATTTCTTCAGATGTACAACTACTTTATTGTTGGAATCTTTGTTTTTGTACATATGTTTTTGCCAATTAAAATGAGTATTTAGTGGATGCGTCTTCTGAAAACAAGGCGAACTAGATTTTAGAATGTTTATTTGCCTCTAGATGATGAGTTTTCGTTTTCTCCATTTCTTTGAGTGGGTGAATTCTTTTGGCTTTTGATTTTGGACGTTTTCTTACCAGCCATCCTTTGAACCTCTGTCTTTCATAGACCTACAATATAATTTTTCTTATCACCAATGAAAGAGGGAGAGAGAAAGAAAATCAACAGTTCTTTCCTTATCCTTAAAAAAAAAAAAAAGAATTGACCGTTCTTTCTGTGTTACAGGCAAGAAACAATGCCCGGATTTTGATCTCTGGATCATTAAGTTTGTTTAGCAACCGGTAGGGAACACCTATAACTTAATTACCAGCAACTAACTTTATTCCTTCTTTATCTATCTCAGGATCTCTTAATAATACAGTTTTTCCTCTTGTTTATAGATTTTTCAAATCTGGGGTGCAGAAGGCTGGGCATCCAACCAAGTAAGTGAAAATGCCTGAACTGGATTGTATTACTTGATAAAAACCTTCATAGCTGTGCAACCATCATGTACATGTAAAATATTATCTAATGGACGGTGGAAAACAATTATGAAGCTGCATCAATTATTAATGAACATTGATGAGAATTTAAATCACATAGCAATTATTGCTATTTAGTATGCTTATGAACTTTTGAAGATACTTGTTGAAGAGAGTATGTTGTGATACAGGTATGATAAGTCAGGCAACGAGAAGTTTTCAATTGAACTAAGCAAATGGGTTTTCCACGAGAGAGGTCATCTTAAGGTGAGGCAGTACTTTAACATTTTTGCATTTTCTACTGTGTATGAATTTGTTCTAGAATTAGATTGTACATGCTAATCTATCCACTGCACATTAATTGACGCTTGTTTTTTAGGCTGTGAATGTTAGGCATCACAAAGTTGGGGAAACTGATGAACCAGCAATTTACAGGATTAATGATGATTTGGTAATTCTAATTTCAGAAATGCTAAATACAGAACGAAATGGAAATACTTGAGTTCTTCTGACCTTTGAATTTCGTTTAAAGGAATATTCCGTTGAGATTTTCGAGTGGTCTGGTAAGGCATGGGAGCCGTATGTCTCCGATGACGTTCAGGTGCAGTTTTACATGATGAGCCCCTATGTTTTGAAAACTTTATCATCCGACAACAAGGTAAAAAAAAAAATCCTATAATTATTTTTTGTTTGATTTTACGTATGGATTTCATTGAACCTTAATATTTATAGCTACTTTCTCTCTTTCTCTTGTAGGGTCTTTACTACACTACCTTTAAAGTTCCGGATGTATATGGAGTATTCCAGTTCAAGGTTGAGTATCAAAAACTTGGATACACTGCCTTATCTCTATCGAAGCAGGTAATGTTTGAGTTTTATCTTGATGCATACTAATTATGGAGGGAATAAGCAAGTTGATGGATCCCATTATTATAGCGAGAAGTAGTTCTGGAAATTATGGCAATTGCTCTTTGTTTAAGATGGAACTCCATCCACGTCGGTCTGAGAGGGGAACGAAACATTTTTGATAAAGGATGTGGAAACCTCTCCCTAGCAGACGCGTTTTAAAACTTTGAGGGGAAACCCGAAAGGAAAAGTCCAAAGAGGACAAACTATCTGCTAGCAGTGGACTTGAGCTATTACATTAAATGTAGGCCTGAATACAGTGCACACTATGAGCGCGTTTTTAGTGTCGGGCAACCTCACTCAGAGCATTCACTCTAGTGTGCCTGCCTGCACCTGCACCTGCACCTGCACATGGGTGAAGTCTTTCATGTTGTGTATGCATATTGTTCTATAACATTATTGTCAGGAAAGCTTATGTGAACACTCATAATATTGCAGATTCCAGTGAGACCCTTTAGGCACAATGAATATGAAAGATTCATTCCCACCGCTTATCCATACTATGGAGCAGCCTTTTCAATGGTATACCTCTTTTAATACCCATTTTTGTATTTATGTGCAGTTTCATGTTTTAAAATGATAATAAGAACTGAATTGTGGTGCAGATGGCTGGCTTCATTATGTTCACTGCTGTTCATCTATACAACAAGTAACCAAAATCACTGTAAGGTGCAGAGCTTATTAGTTATTCTGATTTTGATTTTTTTTTTCTTCTTAATTTTAAATTTTATTTAAGCTGTCTCTTGAATATTGGAATTTTTGAATGCTGATGCTGATACTAAAATTATGGATGCTTTAGTGTTAGTAATTAATTTAGAGAGATATGACAATTTTGTTGAGATTAATCAAATTATTCTCGGAAATTTTATTTTCTTTTA

mRNA sequence

TTTGTGATTATACTTTGAATTGGGGCAATACTTCAACAAGACTATTCTTGTCTTCACAGAACGACTGCAAAAGAGATCTCTGATCCCTCTTCGGACTCTGCCACTTCCTTAAACCCTAGCGCAATGTCGAAATCCCTCGTCGTCTTGATGCTAATCTCTGTTCTTCCATTTCTTTGCTATGCCTTCTCCCCCGAGAATCCCACAGATCGCAAAGTCCTCGTCTTACTCGATGATTTTGCCATCAAATCTACCCATTCTTTGTTCTTCAAGTCCCTCCAGTCCCGTGGTTTCGACCTCGATTTCAAGCTCGCCGATGACCCCAAGATCTCCTTGCAGAGACATGGACGCTATTTCTATGACGCATTGATCTTGTTTGCACCAACAATTGACCGTTTTGGGGGATCCGTTGACCTTGCTGGTATTCTGGATTTTGTCGATTCGGGGCATGATCTAATTCTCGCTGCTGATTCTAATGCATCGGATTTGATCCGAGAAATTGCCACTGAATGTGGTGTTGATTTCGATGAGGATTCAGCTGCTGTAGTCATTGACCACACCAGCTATGCAGTATCAGAGACAGAGGGAGATCACACATTGATTGCCAGTGACGATTTGATCAAGTCTGATGTAATTGTGGGAAGCAAGAAGATTGAGGCTCCTGTTCTTTTCCAAGGGATTGGCCATACCTTAAATCCTGTTAATAACTTGGTGCTAAAAGCTCTCTCAGCATCTCCCTCAGCATATTCTGCGAAGCCAAAGTCCAAGCTATCGAGCCCTCCGCAACTAAAAGGATCTGCTATCTCTCTGGTTTCAGTCATACAGGCAAGAAACAATGCCCGGATTTTGATCTCTGGATCATTAAGTTTGTTTAGCAACCGATTTTTCAAATCTGGGGTGCAGAAGGCTGGGCATCCAACCAAGTATGATAAGTCAGGCAACGAGAAGTTTTCAATTGAACTAAGCAAATGGGTTTTCCACGAGAGAGGTCATCTTAAGGCTGTGAATGTTAGGCATCACAAAGTTGGGGAAACTGATGAACCAGCAATTTACAGGATTAATGATGATTTGGAATATTCCGTTGAGATTTTCGAGTGGTCTGGTAAGGCATGGGAGCCGTATGTCTCCGATGACGTTCAGGTGCAGTTTTACATGATGAGCCCCTATGTTTTGAAAACTTTATCATCCGACAACAAGGGTCTTTACTACACTACCTTTAAAGTTCCGGATGTATATGGAGTATTCCAGTTCAAGGTTGAGTATCAAAAACTTGGATACACTGCCTTATCTCTATCGAAGCAGATTCCAGTGAGACCCTTTAGGCACAATGAATATGAAAGATTCATTCCCACCGCTTATCCATACTATGGAGCAGCCTTTTCAATGATGGCTGGCTTCATTATGTTCACTGCTGTTCATCTATACAACAAGTAACCAAAATCACTGTAAGGTGCAGAGCTTATTAGTTATTCTGATTTTGATTTTTTTTTTCTTCTTAATTTTAAATTTTATTTAAGCTGTCTCTTGAATATTGGAATTTTTGAATGCTGATGCTGATACTAAAATTATGGATGCTTTAGTGTTAGTAATTAATTTAGAGAGATATGACAATTTTGTTGAGATTAATCAAATTATTCTCGGAAATTTTATTTTCTTTTA

Coding sequence (CDS)

ATGTCGAAATCCCTCGTCGTCTTGATGCTAATCTCTGTTCTTCCATTTCTTTGCTATGCCTTCTCCCCCGAGAATCCCACAGATCGCAAAGTCCTCGTCTTACTCGATGATTTTGCCATCAAATCTACCCATTCTTTGTTCTTCAAGTCCCTCCAGTCCCGTGGTTTCGACCTCGATTTCAAGCTCGCCGATGACCCCAAGATCTCCTTGCAGAGACATGGACGCTATTTCTATGACGCATTGATCTTGTTTGCACCAACAATTGACCGTTTTGGGGGATCCGTTGACCTTGCTGGTATTCTGGATTTTGTCGATTCGGGGCATGATCTAATTCTCGCTGCTGATTCTAATGCATCGGATTTGATCCGAGAAATTGCCACTGAATGTGGTGTTGATTTCGATGAGGATTCAGCTGCTGTAGTCATTGACCACACCAGCTATGCAGTATCAGAGACAGAGGGAGATCACACATTGATTGCCAGTGACGATTTGATCAAGTCTGATGTAATTGTGGGAAGCAAGAAGATTGAGGCTCCTGTTCTTTTCCAAGGGATTGGCCATACCTTAAATCCTGTTAATAACTTGGTGCTAAAAGCTCTCTCAGCATCTCCCTCAGCATATTCTGCGAAGCCAAAGTCCAAGCTATCGAGCCCTCCGCAACTAAAAGGATCTGCTATCTCTCTGGTTTCAGTCATACAGGCAAGAAACAATGCCCGGATTTTGATCTCTGGATCATTAAGTTTGTTTAGCAACCGATTTTTCAAATCTGGGGTGCAGAAGGCTGGGCATCCAACCAAGTATGATAAGTCAGGCAACGAGAAGTTTTCAATTGAACTAAGCAAATGGGTTTTCCACGAGAGAGGTCATCTTAAGGCTGTGAATGTTAGGCATCACAAAGTTGGGGAAACTGATGAACCAGCAATTTACAGGATTAATGATGATTTGGAATATTCCGTTGAGATTTTCGAGTGGTCTGGTAAGGCATGGGAGCCGTATGTCTCCGATGACGTTCAGGTGCAGTTTTACATGATGAGCCCCTATGTTTTGAAAACTTTATCATCCGACAACAAGGGTCTTTACTACACTACCTTTAAAGTTCCGGATGTATATGGAGTATTCCAGTTCAAGGTTGAGTATCAAAAACTTGGATACACTGCCTTATCTCTATCGAAGCAGATTCCAGTGAGACCCTTTAGGCACAATGAATATGAAAGATTCATTCCCACCGCTTATCCATACTATGGAGCAGCCTTTTCAATGATGGCTGGCTTCATTATGTTCACTGCTGTTCATCTATACAACAAGTAA

Protein sequence

MSKSLVVLMLISVLPFLCYAFSPENPTDRKVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLADDPKISLQRHGRYFYDALILFAPTIDRFGGSVDLAGILDFVDSGHDLILAADSNASDLIREIATECGVDFDEDSAAVVIDHTSYAVSETEGDHTLIASDDLIKSDVIVGSKKIEAPVLFQGIGHTLNPVNNLVLKALSASPSAYSAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISGSLSLFSNRFFKSGVQKAGHPTKYDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETDEPAIYRINDDLEYSVEIFEWSGKAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTFKVPDVYGVFQFKVEYQKLGYTALSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGFIMFTAVHLYNK

Homology

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BLAST of CmaCh02G004220 vs. ExPASy Swiss-Prot
Match: Q944K2 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS=Arabidopsis thaliana OX=3702 GN=OST48 PE=2 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.0e-190
Identity = 322/430 (74.88%), Postives = 386/430 (89.77%), Query Frame = 0


Query: 6   VVLMLISVLPFLCYAFSPENPTDRKVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLADD 65
           V L+ + +LP L ++FS +NPTDR+VLVLLDD ++KS+HS+FF +L+SRGFDLDFKLA+D
Sbjct: 8   VALISVFLLPLLSFSFSVDNPTDRRVLVLLDDLSLKSSHSIFFNTLKSRGFDLDFKLAED 67

Query: 66  PKISLQRHGRYFYDALILFAPTIDRFGGSVDLAGILDFVDSGHDLILAADSNASDLIREI 125
            K++LQR+G+Y YD LI+FAP+ +RFGGS+D   I DFVDSG DLIL+AD+ ASDLIR I
Sbjct: 68  SKLALQRYGQYLYDGLIIFAPSTERFGGSLDSKSIADFVDSGRDLILSADTAASDLIRGI 127

Query: 126 ATECGVDFDEDSAAVVIDHTSYAVSETEGDHTLIASDDLIKSDVIVGSKKIEAPVLFQGI 185
           ATECGVDFDEDS+A+VIDHTS++VS+ +GDHTLIA+DDL+KSDVI+G  KIEAPVLF+G+
Sbjct: 128 ATECGVDFDEDSSAMVIDHTSFSVSDVDGDHTLIAADDLVKSDVILGKTKIEAPVLFRGV 187

Query: 186 GHTLNPVNNLVLKALSASPSAYSAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISGS 245
            H+LNP NNLVLK LSASPSAYSA P SKLSSPPQL GS+ISLVSV+QARNNAR++ISGS
Sbjct: 188 AHSLNPTNNLVLKVLSASPSAYSANPSSKLSSPPQLTGSSISLVSVMQARNNARVVISGS 247

Query: 246 LSLFSNRFFKSGVQKAGHPTKYDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETDE 305
           + LFS+R  +SGVQKAG P +Y+KSGNE+F  ELSKWVFHERGHLKA N+ HH+VGETDE
Sbjct: 248 VQLFSDRLIRSGVQKAGSPNQYEKSGNEQFVTELSKWVFHERGHLKAGNLVHHRVGETDE 307

Query: 306 PAIYRINDDLEYSVEIFEWSGKAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTFK 365
           PAIYRI DDLE+SVEI+EWSGK+WEPYV++DVQVQFYMMSPYVLKTLS+D KGL++T+FK
Sbjct: 308 PAIYRIKDDLEFSVEIYEWSGKSWEPYVANDVQVQFYMMSPYVLKTLSTDKKGLFHTSFK 367

Query: 366 VPDVYGVFQFKVEYQKLGYTALSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGFI 425
           VPDVYGVFQFKVEY+KLGYT LSLSKQIPVRP+RHNEYERFIPTAYPYYGA F+ MAGF 
Sbjct: 368 VPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAYPYYGACFTTMAGFF 427

Query: 426 MFTAVHLYNK 436
           +F+ V+LY+K
Sbjct: 428 VFSFVYLYHK 437

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BLAST of CmaCh02G004220 vs. ExPASy Swiss-Prot
Match: Q6ZLK0 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS=Oryza sativa subsp. japonica OX=39947 GN=OST48 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 5.0e-182
Identity = 313/431 (72.62%), Postives = 371/431 (86.08%), Query Frame = 0


Query: 5   LVVLMLISVLPFLCYAFSPENPTDRKVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLAD 64
           L + + +++L     A     P  R+VLVL+DD A++S+HS FF SLQ RGFDLDF+LAD
Sbjct: 9   LALAVALALLVVTAAAADEGGPRGRRVLVLVDDLAVRSSHSAFFASLQGRGFDLDFRLAD 68

Query: 65  DPKISLQRHGRYFYDALILFAPTIDRFGGSVDLAGILDFVDSGHDLILAADSNASDLIRE 124
           DPK+SL R+G+Y YD L+LFAP+  RFGGSVD   IL+F+D+GHD+ILAADS+ASDLIR 
Sbjct: 69  DPKLSLHRYGQYLYDGLVLFAPSTPRFGGSVDQNSILEFIDAGHDMILAADSSASDLIRG 128

Query: 125 IATECGVDFDEDSAAVVIDHTSYAVSETEGDHTLIASDDLIKSDVIVGSKKIEAPVLFQG 184
           IATECGVDFDED  A+VIDH +YA ++ EGDHTLIA DDLI+SDVI+GSKKIEAPVLF+G
Sbjct: 129 IATECGVDFDEDPEAMVIDHINYAATDAEGDHTLIAGDDLIQSDVILGSKKIEAPVLFRG 188

Query: 185 IGHTLNPVNNLVLKALSASPSAYSAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISG 244
           IGH +NP N+LVLK LSASPSAYSA PKSKL+SPP L GSAISLVSV+QARNNAR+LISG
Sbjct: 189 IGHAVNPSNSLVLKVLSASPSAYSANPKSKLASPPSLTGSAISLVSVMQARNNARVLISG 248

Query: 245 SLSLFSNRFFKSGVQKAGHPTKYDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETD 304
           SL LFSNRF KSGVQKAG   +++K+GNE+F  E SKWVFHERGHLKAVNV+H+KVGET+
Sbjct: 249 SLDLFSNRFLKSGVQKAGSKIRHEKAGNEQFVTETSKWVFHERGHLKAVNVKHNKVGETN 308

Query: 305 EPAIYRINDDLEYSVEIFEWSGKAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTF 364
           EP +YRINDDLEYSVEI+EWSG +W+PYV+DDVQVQFYMMSPYVLKTLS+D KG++ T+F
Sbjct: 309 EPGMYRINDDLEYSVEIYEWSGTSWKPYVADDVQVQFYMMSPYVLKTLSTDKKGVFSTSF 368

Query: 365 KVPDVYGVFQFKVEYQKLGYTALSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGF 424
           KVPDVYGVFQFKVEYQ+LGYT LSLSKQIPVRP+RHNEYERFI +AYPYY A+FS M  F
Sbjct: 369 KVPDVYGVFQFKVEYQRLGYTGLSLSKQIPVRPYRHNEYERFITSAYPYYAASFSTMGAF 428

Query: 425 IMFTAVHLYNK 436
            +F+ V+LY+K
Sbjct: 429 FIFSFVYLYHK 439

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BLAST of CmaCh02G004220 vs. ExPASy Swiss-Prot
Match: Q6NYS8 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS=Danio rerio OX=7955 GN=ddost PE=2 SV=2)

HSP 1 Score: 377.5 bits (968), Expect = 2.1e-103
Identity = 197/409 (48.17%), Postives = 281/409 (68.70%), Query Frame = 0


Query: 28  DRKVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLADDPKISLQRHGRYFYDALILFAPT 87
           D K LVLLD+  I+ THS+FF+SL  RGFDL FK ADDP +SL ++G++ YD LILF+P+
Sbjct: 29  DGKTLVLLDNPNIRDTHSIFFRSLADRGFDLTFKTADDPGLSLIKYGQFLYDHLILFSPS 88

Query: 88  IDRFGGSVDLAGILDFVDSGHDLILAADSNASDLIREIATECGVDFDEDSAAVVIDHTSY 147
           ++ FGG++++  I  F+D G ++++AA S+  D +RE+ +ECG++FDE+  A VIDH +Y
Sbjct: 89  VEDFGGNINVETITAFIDGGGNVLVAASSDIGDPLRELGSECGIEFDEEKTA-VIDHHNY 148

Query: 148 AVSETEGDHTLIASD--DLIKSDVIVGSKKIEAPVLFQGIGHTLNPVNNLVLKALSASPS 207
            +S+  G+HTLI +D  +L+K+  IVG K  + PVLF+G+G   +P N LVL  L+ S +
Sbjct: 149 DISD-PGEHTLIVADPENLLKAPTIVG-KPTDKPVLFKGVGMVADPDNPLVLDILTGSST 208

Query: 208 AYSAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISGSLSLFSNRFFKSGVQKAGHPT 267
           +YS  P   ++  P   G    L++ +QARNNAR++ SGSL  FS+ FF S VQKA   +
Sbjct: 209 SYSYFPDRPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLHFFSDAFFNSAVQKAATGS 268

Query: 268 K-YDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETDEPAIYRINDDLEYSVEIFEW 327
           K Y+++GN+  +  LS+WVF E G L+  +V HH VGE+  PA Y + D +EY + I   
Sbjct: 269 KRYEQTGNQDLAEALSRWVFKEAGVLRVGDVTHHPVGESTPPAAYTVTDLVEYGIVIEML 328

Query: 328 SGKAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTFKVPDVYGVFQFKVEYQKLGY 387
           SG  W P+  DD+Q++F  + P+V +T    N G Y   FK+PDVYGVFQFKV+Y +LGY
Sbjct: 329 SGGKWVPFDGDDIQLEFVRIDPFV-RTYLKKNGGKYSVQFKLPDVYGVFQFKVDYNRLGY 388

Query: 388 TALSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGFIMFTAVHLY 434
           T L  S Q+ VRP +H +YERFIP+A+PYY +AFSMMAG  +F+ V L+
Sbjct: 389 THLYSSTQVSVRPLQHTQYERFIPSAFPYYASAFSMMAGLFVFSVVFLH 433

[Close]

BLAST of CmaCh02G004220 vs. ExPASy Swiss-Prot
Match: Q641Y0 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS=Rattus norvegicus OX=10116 GN=Ddost PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.3e-102
Identity = 192/409 (46.94%), Postives = 276/409 (67.48%), Query Frame = 0


Query: 30  KVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLADDPKISLQRHGRYFYDALILFAPTID 89
           + LVLLD+  ++ THSLFF+SL+ RGF+L FK ADDP +SL ++G + YD LI+F+P+++
Sbjct: 31  RTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVE 90

Query: 90  RFGGSVDLAGILDFVDSGHDLILAADSNASDLIREIATECGVDFDEDSAAVVIDHTSYAV 149
            FGG++++  I  F+D G  +++AA S+  D +RE+ +ECG++FDE+  A VIDH +Y V
Sbjct: 91  DFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTA-VIDHHNYDV 150

Query: 150 SETEGDHTLIASD--DLIKSDVIVGSKKIEAPVLFQGIGHTLNPVNNLVLKALSASPSAY 209
           S+  G HTLI +D  +L+K+  IVG   +  P+LF+G+G   +P N LVL  L+ S ++Y
Sbjct: 151 SDL-GQHTLIVADTENLLKAPTIVGKSSLN-PILFRGVGMVADPDNPLVLDILTGSSTSY 210

Query: 210 SAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISGSLSLFSNRFFKSGVQKAG-HPTK 269
           S  P   ++  P   G    L++ +QARNNAR++ SGSL  FS+ FF S VQKA     +
Sbjct: 211 SFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKAAPGAQR 270

Query: 270 YDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETDEPAIYRINDDLEYSVEIFEWSG 329
           Y ++GN + ++ LS+WVF E G L+   V HH+VGET  P  Y + D +EYS+ I + S 
Sbjct: 271 YSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIEQLSN 330

Query: 330 KAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTFKVPDVYGVFQFKVEYQKLGYTA 389
             W P+  DD+Q++F  + P+V +T      G Y   FK+PDVYGVFQFKV+Y +LGYT 
Sbjct: 331 GKWVPFDGDDIQLEFVRIDPFV-RTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTH 390

Query: 390 LSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGFIMFTAVHLYNK 436
           L  S Q+ VRP +H +YERFIP+AYPYY +AFSMMAG  +F+ V L+ K
Sbjct: 391 LYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFLFSVVFLHMK 435

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BLAST of CmaCh02G004220 vs. ExPASy Swiss-Prot
Match: B1H3C9 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS=Xenopus tropicalis OX=8364 GN=ddost PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 5.1e-102
Identity = 188/409 (45.97%), Postives = 276/409 (67.48%), Query Frame = 0


Query: 30  KVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLADDPKISLQRHGRYFYDALILFAPTID 89
           + LVLL++  ++ THSLFF+SL  RGFDL F+ ADDP +SL ++G + YD LI+F+P+++
Sbjct: 27  RTLVLLENINLRETHSLFFRSLSDRGFDLSFRTADDPSLSLIKYGEFLYDNLIIFSPSVE 86

Query: 90  RFGGSVDLAGILDFVDSGHDLILAADSNASDLIREIATECGVDFDEDSAAVVIDHTSYAV 149
            FGG++++  I  F+D G  +++AA S+  D +RE+ +ECG++FDED  A VIDH +Y +
Sbjct: 87  DFGGNINIETISSFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEDKTA-VIDHHNYDI 146

Query: 150 SETEGDHTLIASD--DLIKSDVIVGSKKIEAPVLFQGIGHTLNPVNNLVLKALSASPSAY 209
           S+  G H+LI +D   L+K+  IVG   +  P+LF+G+G   +P N LVL  L+ S ++Y
Sbjct: 147 SD-PGQHSLIVADSESLLKAPTIVGKAPLN-PILFRGVGMVADPDNPLVLDILTGSSTSY 206

Query: 210 SAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISGSLSLFSNRFFKSGVQK-AGHPTK 269
           S  P   ++  P   G    L++ +QARNNAR++ SGS+  FS+ FF S VQK AG   +
Sbjct: 207 SFFPDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSMDFFSDAFFNSAVQKAAGGSNR 266

Query: 270 YDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETDEPAIYRINDDLEYSVEIFEWSG 329
           Y K+GN + ++ LS+WVF E G L+   V HH+VGE+  P+ Y + D +EYS+ I + S 
Sbjct: 267 YAKTGNYELAVALSRWVFKEEGVLRVGQVSHHRVGESSPPSAYTVTDLVEYSIVIEQLSN 326

Query: 330 KAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTFKVPDVYGVFQFKVEYQKLGYTA 389
             W P+  DD+Q++F  + P+V +T    N G Y   FK+PDVYGVFQFKV+Y +LGYT 
Sbjct: 327 GKWVPFDGDDIQLEFVRIDPFV-RTFLKKNGGKYSVQFKLPDVYGVFQFKVDYNRLGYTH 386

Query: 390 LSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGFIMFTAVHLYNK 436
           L  + Q+ VRP +H +YERFIP+AYPYY +AFS+M G  +F+ V L+ K
Sbjct: 387 LYSTTQVSVRPLQHTQYERFIPSAYPYYASAFSVMFGLFIFSIVFLHMK 431

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BLAST of CmaCh02G004220 vs. TAIR 10
Match: AT5G66680.1 (dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit family protein )

HSP 1 Score: 667.5 bits (1721), Expect = 7.1e-192
Identity = 322/430 (74.88%), Postives = 386/430 (89.77%), Query Frame = 0


Query: 6   VVLMLISVLPFLCYAFSPENPTDRKVLVLLDDFAIKSTHSLFFKSLQSRGFDLDFKLADD 65
           V L+ + +LP L ++FS +NPTDR+VLVLLDD ++KS+HS+FF +L+SRGFDLDFKLA+D
Sbjct: 8   VALISVFLLPLLSFSFSVDNPTDRRVLVLLDDLSLKSSHSIFFNTLKSRGFDLDFKLAED 67

Query: 66  PKISLQRHGRYFYDALILFAPTIDRFGGSVDLAGILDFVDSGHDLILAADSNASDLIREI 125
            K++LQR+G+Y YD LI+FAP+ +RFGGS+D   I DFVDSG DLIL+AD+ ASDLIR I
Sbjct: 68  SKLALQRYGQYLYDGLIIFAPSTERFGGSLDSKSIADFVDSGRDLILSADTAASDLIRGI 127

Query: 126 ATECGVDFDEDSAAVVIDHTSYAVSETEGDHTLIASDDLIKSDVIVGSKKIEAPVLFQGI 185
           ATECGVDFDEDS+A+VIDHTS++VS+ +GDHTLIA+DDL+KSDVI+G  KIEAPVLF+G+
Sbjct: 128 ATECGVDFDEDSSAMVIDHTSFSVSDVDGDHTLIAADDLVKSDVILGKTKIEAPVLFRGV 187

Query: 186 GHTLNPVNNLVLKALSASPSAYSAKPKSKLSSPPQLKGSAISLVSVIQARNNARILISGS 245
            H+LNP NNLVLK LSASPSAYSA P SKLSSPPQL GS+ISLVSV+QARNNAR++ISGS
Sbjct: 188 AHSLNPTNNLVLKVLSASPSAYSANPSSKLSSPPQLTGSSISLVSVMQARNNARVVISGS 247

Query: 246 LSLFSNRFFKSGVQKAGHPTKYDKSGNEKFSIELSKWVFHERGHLKAVNVRHHKVGETDE 305
           + LFS+R  +SGVQKAG P +Y+KSGNE+F  ELSKWVFHERGHLKA N+ HH+VGETDE
Sbjct: 248 VQLFSDRLIRSGVQKAGSPNQYEKSGNEQFVTELSKWVFHERGHLKAGNLVHHRVGETDE 307

Query: 306 PAIYRINDDLEYSVEIFEWSGKAWEPYVSDDVQVQFYMMSPYVLKTLSSDNKGLYYTTFK 365
           PAIYRI DDLE+SVEI+EWSGK+WEPYV++DVQVQFYMMSPYVLKTLS+D KGL++T+FK
Sbjct: 308 PAIYRIKDDLEFSVEIYEWSGKSWEPYVANDVQVQFYMMSPYVLKTLSTDKKGLFHTSFK 367

Query: 366 VPDVYGVFQFKVEYQKLGYTALSLSKQIPVRPFRHNEYERFIPTAYPYYGAAFSMMAGFI 425
           VPDVYGVFQFKVEY+KLGYT LSLSKQIPVRP+RHNEYERFIPTAYPYYGA F+ MAGF 
Sbjct: 368 VPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAYPYYGACFTTMAGFF 427

Query: 426 MFTAVHLYNK 436
           +F+ V+LY+K
Sbjct: 428 VFSFVYLYHK 437

The following BLAST results are available for this feature:

BLAST of CmaCh02G004220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q944K2	1.0e-190	74.88	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS...	[more]
Q6ZLK0	5.0e-182	72.62	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS...	[more]
Q6NYS8	2.1e-103	48.17	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS...	[more]
Q641Y0	2.3e-102	46.94	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS...	[more]
B1H3C9	5.1e-102	45.97	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS...	[more]

BLAST of CmaCh02G004220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66680.1	7.1e-192	74.88	dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit fami...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005013	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48kDa subunit	PFAM	PF03345	DDOST_48kD	coord: 31..434 e-value: 1.3E-129 score: 432.7
IPR005013	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48kDa subunit	PANTHER	PTHR10830	DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT	coord: 1..435
None	No IPR available	PANTHER	PTHR10830:SF2	DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT	coord: 1..435

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G004220.1	CmaCh02G004220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018279	protein N-linked glycosylation via asparagine
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0008250	oligosaccharyltransferase complex
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0016740	transferase activity