CmaCh02G002670 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G002670
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionUBC core domain-containing protein
LocationCma_Chr02: 1204022 .. 1213151 (-)
RNA-Seq ExpressionCmaCh02G002670
SyntenyCmaCh02G002670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTTCATGAGGTTTGAAGGGCTCCTCTCGTAGACTCCAAGACGTTCTTGTGCTTGAAAGGTTATTCAAATCATTAGCTAAAGATGCCATTTAGACCTTTAGAGAACCAACCTCATCATTCCTACAAGTCTTTTGTATTATTGAGAATGGCTCTTACGAGCTCACCTGATTCTCATCCATGCTAATTTTTAGTATCATGCATGACATTACAACTCTTCATTAAAATTTGAGTACATTTCAAATCATTCTCCTTAGGAAAGCAACTATCGTCATGCATTTAAGACATGTTCTAAATTATATCTCGCACATGTGAATGTAGACCAACATAAACTATTGACATATGATCACAAACTTCAAGGCATTAAGAATTAAATACATTAATATAGGCAGTGTCTCGACGCCTCAAACAAAAATCCCCATTACGAGATGCATACATGCTTTTGTCTAATGGAACCCAAAATTCGTAGCGTTGAGACAACCAAAACAACGTCTCAGGTGAAATATGAGATAATAATTTTGTTCGTGAATTATATTTGAAAAGGTTTTGTTTGTGAACCAACCAAACTTTATCCTTTACAATTTTTAAAATATACCTCGGTTAACTTAACCTAGTTTAAAATAAAAATATTATTTTTTTTTATTAAATTCTTAGAAAAATGTTAACTTGCCTTGTAAATTATAAACTTCCTTTCAATATTAGAACTCCCCAAGAAAAGCACCTTTCATTCCCTTGCTTCAAATCCAAACTTACATATAACTATGTTTAAATTTATTTTTTTTAAAATTTATCTCTATTTACACTTTATATATTTTATTATTAAATACTAATAAATAAATAAAAATATGTTTTTATAATAAATTTTCTACTAATTACTTAGTTTAGAATTTAATTAAAATTTTTGTACGATGATTAATTTCAAACATACACAATACTTTTTTTTAAAAAAAATTCAATTTTACGCCAATTATATATATATTAATTAGTAAAAATTATTATTAAAAGTTTTGAAACTAAAATATTAATAGAAAAAAAGTTGGGTTTAAATATATATCCAAATAAATAAATAATAAAATTGGTATAATATAATAAATATATTTTTTAAGTTAAAAAATAATTATTTTTGCAACGGGGCTTGGTCCTAATTTTGAACGGCGGTAATGGATCACGATTACCATACCCATCAACGGACACAAATATATAAGAAGAAGGCCCCCAGGTTTTTATTTATTCCCATAACCAACCCCCATATTATAGGTATTTACAAACCCACCCCCACGGTAACTCTCTTACCAGCTCCTCCTTCTAATTCTTTCATAATATATTATATTCTCTGGTTCGAAAACGGAGTTCGCAGGAACCTCCGTTCAAAATCAAAGGCCTCCTTTTCTTCATCGCATCCTCTTCTTTCCCTCCAAGTGACGGCGATTTTCAGATTGCCTTTCTCTTTGCAGCTCATTTTTGTCTGCCTCCGAGCAGCCTCAGGTTCTCATTTCATTAGCTCGTTTGTTTTGGTTACTTGATTGGATTGAGCTTTTGGATGGAATATTTTTTCTTTATTGTTTGGATTACTGGTGGGATGTTTGAGTTTAGCTCGAGGGATTGATCTGCGAAGGTCTCGAATCGTGGTCTTCAGCTTCTTTAGTTAGTTCGTTAGTTTGTTTGTTCTTTTTTTCATTTCGATTAATGGTTTTGCTGGTAATTATGGCCTGCTCTGAGCATTTGAATGCGAGAACGGGAGAGGTGTAAATGGTTTTAGCCATTATTTCTTCTTCTTCTTGCGTTTGATTCAAGTAACTTGATGATTTTAACGAAGACGTCCATTGGATGCCTTCTTAATTCTGTCGTCTTGCTAAGCATATTAGCAAATGAACCCAGAATTTTTCATTTCTGTTTGTGTTGGTCAATCGTCTTTCCTGGGTTTTTGGGTTTTTTTTGAATTTTAGATCTGAAAATCTATAATGGAGATGGTTGAGATATACTACTGGAAACATATTTGATGACTGCTCTAAGTAGCAACAAAAAGCTTCTTGCAAAAATATGTCAACCTCCTGATTCTTTATTCAAATATGCTATAAATGTCCACTTCTTTCTGCTCTGTTCATTCTGCAACTTTTCCCTATTTGTTGAAAATATGAAGGAAAAAGGAGCAGTGAGCTTATTTGACTTTATTGTTGCTTAGAGTAAAATTACAGCGACTGGTTTGTTCATACTTACAGACTAATGGGCCATGGATTTTGTTTGTTCACTTTCTTGGCTGGCTAGATAGGCATCTAGGAAGCTTGAATTCTCTTATTACATGCAGAGTTGGGAATCCAACATGGACATCTTTGGTATATGATTTTGAAGTTTTAATTTATTTAAATGTTGCACGCGGTGAGGATGCCCAAAGGATTCATTGGGGACCCTGGGCGCGTTAGGGAGGTGCATCTGGTTTAGTACAATAATATATATAATAAAAGAAAAAAATGTTGTTCTGTTTTGGTTCTTATATTTGACCTTTGACAAATGTACTTTACAGGACTGAAATGAATATTATCCCAATGTACTTGAGTTACATATGTAAGTCATTTCTGTACTCATCCTTCTTCCTTCTTTCTTTCCAGATGGGGCAACAGGATTCTGTGGACAAGGAGTCTCTTACGGATACTTGCGATAGTTACATGAATAGGGATGGTGTTTCTACAAGTGGAAATATTCACATGCCAACTATAAATAATGATGAAGCTAGAAGAATGAATGAGAATAAAAGCGATAAACATAATGTTCCACATGTATATAGACGAGATATTGTAAAAAGCAAAGGCAGTGGTATGATAGGGATTGTGACTGAAGTTGCTGGTGATGCTGATTCAGATACTGACATTACTGATGATGAAGATGACTATGATGATGGTGAGGATGGTCTTGATGATGATGGTTGTGATGATTATGGGGAGAACGAAGGTCAAAACAAGGAAAACTATGACGATGACACTAATGCTAAAAATTCAAACAGAGATAGTTATAAGAATCAACCCCTTACTGCTGATGAAGTTCGAGTCCTCTGGATGGATGAAAGTGAGACTACTCAGAATGTTAATGATTTAACAGTTATAGATAGGGGTTTTTTACATGGTGATTTTGTTGCTGCTGTTTCCAATCCTACAGGGCAAGCAGGTGTTGTGGTAGATGTAAATATCTCTGTTGACTTGTTAGTTCCTGATGGATCAGTTGTGAAAAATATATCATCTAAGGACTTGATACGTGTGAGAGATTTCACGGTGGGGGATTATGTGGTCCTTGGTCCGTGGTTGGGTAGAATTGATGATATCTTGGATAATGTGACTGTGATGTTTGATGATGGCTCCAAGTGTAAAGTCACAAAGGCAGAGCCATCGCGGCTCAAACCAGTTTCTAAGAATATCCTTGAAGATGCAAGCTTTCCTTACTATCCTGGCCAGCGGGTGAGGGCGAGTTCTTCAACAGTTTTTAAGAATTCTAAATGGCTTTCTGGTTTATGGAAACCTAATCGATTGCAAGGTACTGTCACTAAAGTTACAGTTGGATCAGTGTTCATTTATTGGATAGCATCTGCTGGATATGGACCAGATTCTTCTACCACCCCAGCTGAAGAACAGAGTCCAAAGAACTTGAGACTATTAACTTGTTTTTCACATGTGAATTGGCAATTGGGTGACTGGTGTCTTCTTCCCTCCTCATTCTCCTCTGGCTCGACTAAGGACTCATCACAAACAGAACTTTCTGATTCAGTGACCAATACGTTAGATTACGCACAAACAGTGGGAGCATGCGATTCAGAGGATACTGTGCTAGATGAATTAAATGGGAGAACTGAATCCACTGATCTTGACTCCATTAGTGCATGTAATGGAAATTATAGGGATCCAGAATGTCATATTTTGCCGGAATCTAGTAGTTCTAGAGCAACAAAGGAGAATACCCATGAGACTTGGCCTCTACATCGCAAAAAAATTCGTAAAGTTGTCATCAGGAGAGATAAGAAGGCTAGAAAGAAAGATGACAATTTTGAGAGGGCCCTTTTAATCATCAATACTTCGACAAAAGTTGATGTTGTATGGCAGGATGGCCGGACAGAACTTGCGCTGGATTCAACTGGCTTGATTCCAATTAATCCAGGTGATCATGAATTTGTTCCAGAACAGTATGTGGTTGAGAAGGCCTCTGATAATGATGATGATGTTACAGAAAGCAGGCGAGTTGGAGTTGTGAAAAGTGTTCATGCAAATGAGCGAACAGCCTGTGTAAGGTGGTTAAAACCAGTCTCCAGGGCAGAGGATCCTCGAGAATTTGACATAGAAGAAGTTGTTAGTGTGTATGAGCTAGAAGGGCATCCAGATTATGACTACTGTTATGGTGATGTTGTTGTTCGTTTGTCACCTGTTTCTGATTCTATAGAAGCTCTGTCTTCTGAAAACAATACTGAAGAATTAAATCATCAAAGTTCAACCAATGGGGTGATGAGTTGTACAGAAAAGGTTTCAGGAAGCCTGAAAATAGAATATCCATCAAGTAGTGATGATTTCTTTGACTTCTCAGATCTCTCTTGGGTTGGAAATATAACGAATCTTAAGAATGGTGATATTGAAGTTTTTTGGGCCAATGGCATGGTTTCAACGGTATTTTTCATTTCCTAATTCTTTTGAAAATGATACTTGGTTGCAAACGTAAAAAACAAGACCAAATGGTTTATGATCTTCTTCTAACAGGAAGAGTGCATTATCATTGTAATTAAACAAATTTATTATAAGGGCAGGTTAGGGAGATGTTTTTCCTGTATTTTCGTAGGAATTGATTGTTATTTTTATGTTTGGTATCTGCTTATTTATTGGCTTTTAAATTAGTTGATCTTGAAATATTAAAAATGTTACTTTTTTTTTTTTTGTCTTGATAGTCTATAATCTAACATTCATATGTGGTAACTGATTTAATTTTTAATGGTTTTCACTTTTCTCACGAGTTTAGAAACTCAAATGCTGAAGTGTTTATTATTTCGCTAAGGTTGGCCCTCAAGCAATTTATGTTGTTGGTCGAGATGATGATGATGAATCAATTGGTGCTGGAAGTGAAATAAGTAACGGTGCTGCAAGTTGGGAAACAATTGATGATGATGATGATGATGATGAAATGGATGTTGAGAACGCCAAGGAGGTAATATTCTTTTGGTACACTAAAGTTAGCATATTCTTTTGAATGGTTCTGATGTTGAGGATTGTTGGGAGATGAGTCTCATATTGGCTAATTAATGGGAATATCTTGAGTTTCTAAGTAAGGAATACTATCTCCATTGGTATGAGGCCTTTTGGGGAAACCAAAAGCAAAACCATGAGAGCTTATGCTCAAAGTGGACAATATTATTACTGTTGTGGAGGGTCGTGGTTCCTAACATGGCATTAGAGCCGTGCCCTCAACTTAGCTATGTCAATAGAATCCTCAAGTGTTGAACAAAGAAGTTGTGAACCTCGAAGGTGTAGTCAAAAAGTGACTCAAGTGTCGAACAAAGGTTGTACTTTGTTCAAGAGCTCCAAAGAAGGAGTCAAGCCTCGATTAAGGAGAGGTTGTCTGAGGGCTCCATAGGTCTTAGGGGAGGCTCTATGGTGTACTTTTTTCGAGGGGAGAATTGTTGAGAATTGTTGGGCTGATTCTCATATTGGCTAATTAAGGGAAGATCATGAGTTTATAAGTAAGGAATGCTATCTCTATTGGTATGAAGCCTTTTGGGAAAACCAAAAGTAAAATCATGAGAGCTTATACTCGAAGTGGACAATATCATACCATTGTGGAGGGTTGTGGTTCCTAACATCTAATAATATAATCTCTAGATTTTCTGTCAAAGAAACTAAAGAATAAAACTAGAAGTAACATGTAATTATGAGGGCCGGTATGGACTTACCTTTGTTTTAATCATGCATATTAGATGTAAAAGGTCAGTCTGAATTCCTGTCTCTTTACTTGTAATCTTCTTTAAATTATGGGTTTCAAAATTTATTTTGATAGACTCTCAGTCTTTCACTCACTCTCAATAATATCATTTTTTTTTTTTCAGTTTCTGCTTGCAAGGTTTTGGCAAAAATTTCATATCATTTTTTATTAGTGACCCTCCATATGTTATGCCCTCTTGTGCTGATTTTTTGAAAGTTCGGCAGTTCTTATGAATTGAAATCTGATGTCATTTTGCCCTTCCATTGTTCATTTATTTGTCTTTGCTTGTAACGTTGTTATCTACAGTTTTAAGAATAATTACACCTGATATGTATTAAGGCATCGCGAATAATGACCCACGTCAATGTCTCTAGGATATTGAGCTGCAAGATACTGGAGTGGATTCTGAAGAGGAAGAGAGTGAACAGCATAATTCAGGAAGAAGTCTAGCTCTTTCTGTTCCACTTGCTGCGTTTCGATTTGTTACCAGATTGGCTACTGGAATATTCTCACGAGGACCAAGAAATCGAGAATCAATGGATTTGGATTCCCACAGTGAAAGTGACACTCAATCCTTGGAAATTCAAGCTTTGGAGGAAATAGATTCTGGGCTTCAGTCTAGCTCTCTTAAATCCAATTCTTTTACTGCTTCTGATATGAACTCTGACTGTGGAAGAGGAGAGGACGATGATGCCTCAGAGCCATCAAAAGTGTTAGTATCAGCAGAAGCTTCAAGCAACCTGAGGACTGCTCAATCAGATGGGTCAACATGCCATGAGGATGGCACCTGCAGCTTCAAGGGCTTTGATATCGCTAAAGATCCTTTGGACCATTATTTTCTTGGTACGAATGGTCAAGTAAGTAATTTACAAAATTCTCCAAACCTTGTTATATTTGTGAAGTTTCGATACCACTTTGATGCTCTAAGAATACTTCTGTGCACGGTTCTTGTTTTAGGGTTGCACGGCTCTTGTTTTAGGGTCTTGTTCTTCATGTTCATCAATACTTTTGGGTTTCGGGTTGTCCAGATTGGTTTACACGTTCTTACTTGAAAGATTCTCGCACAAGTCTGAATGGTTGAACTTGTTGAATGCAGACTATTAACGGAAGGAAATGGCTTAAGAAAATCCAGCAAGATTGGACTATCCTCCAGAATAATCTTCCAGGTAAACAATGCCCATCTTTGCTTCATTGTGTCGCGTCTCGTGAGATTGACATTCATGTATCTGTTAGCTGAGCCACGAATGAATTCTGTAACAGATGGGATATACGTTCAAGTCTATGAGGATCGAATGGACCTTTTAAGAGCCGTCATTGTTGGGGCCTATGGAACCCCTTACCAAGATGGCCTTTTCTTCTTCGATTTTCACCTCCCACCAGATTATCCCGACGTTCCTCCTGTGAGTCATAGCATTAAGTCACTTCTATAATCTGGTTTGTCTTATATGGTAACTTAATGATGGGATTTGTTATGATGCAGTCAGCATATTATCATTCAGGTGGGTGGCGGATAAATCCCAATCTTTATGAGGAAGGCAAAGTGTGCCTTAGCCTTTTAAATACTTGGACTGGCAGAGGAAATGAAGTGTGGGATCCAAAGTCCTCTAGCATCCTTCAAGTGTTGGTATCGCTCCAGGGGTTAGTGTTGAATTCTAAACCATATTTTAATGAAGCGGGATATGACAAACAAATTGGGACAGCAGAAGGAGAGAAGAATTCCTTGTCGTACAATGAAAACACCTTCTTGTTGAACTGCAAGACAATAATGTATCTTATGAGGAAGCCTCCCAAGGTGGGTATTACTGTTTATTTTCATTTGAATATCTTTGTTGAGAACTTTTTCTGCACCACACAATCTTCAAACGTCATATGCTGAAAGGAGGATGGTGAGATCCGACATCGATTGGAGGGAGGAACAAGTGTCAGCGAGGATGCTGGGCCCCGAAGGTGGGTGGATTATGAGATCACACATTAGTTGGAGAGGAGAATGAAACCTTCTTTATAAGGGTGTGGAAACCTTTCCCTAGCAGATGCGTTTGAAAAACTTTGAGGGAAAGGGTGTGGATACCTTTCCTTAGTAGATGCGTTTGAAAAACTTTGAGGGGAAGCCCAAAGAGGACAATATCTGTTAGCGGTGGGCTTGAGCTATTATAGATGATATCATAGCCAGACATTGGGCGGTGTGCCAGTGAGGACGCTGGGCTACGAAGGTGGGTGGATTGTGAGATCCCACGTCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACTTTCCTCAAGCAGACTTGTTTTAAAAATCTTGAAGGGGAGCCCGAAAGGGAAATTCCAAAGAAAACAATATCGGCTAGCGGTGGGCGTAGGCTATTACGGTGTTTCTTTTTTCTTTTTCCTCTTTAATGGACAACCATGGGTTTGAAGAACTTGTGATTGGTCGGTGGATTTCATAACTTGAGTGAATTTATGTATGCTGTACACAGAAAGTTCATGATGCAATGACTGCTAGTGATATATTAGCTATAAATATTATGTTTGACCAATTGATTTTGTTCATGAATAGGACTTTGAAGAACTCGTTAAAGAACATTTCAGGAAGCGTGGATATTACATCCTGAAGGCCTGTGATGCATACATGAAAGGTCACTTAATTGGCTCTCTCACAGAAGATGCCTCTATAAGAGAAGAAAGCGATCCCAACTCCACGTCAGTCGGGTTTAAGCTTATGCTCGCTAAGATTGTTCCGAAGCTGTTCTCGTCGTTGAACGAAGTCAGAGCTGATTGTCGTGATTTTAAGCATTTTCAACAGTTGTAGCATTACGAAAAACTCCAGATGACAAGGTTGTCATCATTTACACATAGCTGACAAGGTAATCTTTTTCTTTTCTTTTCTTCTATTCATTCCCATTCATGTTAAAGCCTTCTTGTCTTTACCCATTATGATCAATGATGCACCTTGTTTATAAGTTTTGAACTGGGGGCCTCATGAACTTCAAAAGCTTCCTAGAATTCTAGGATCTGGGTTTTTTGTGACAATTCTTTGGTCTGTGATCCATTTTTTTCCTTAGAAAAAACGAAGGAAAGTCAAATTACATTGCAAGTTAGGAGTTCGTTGGTCTTGTTCTGTCGTAGGTTGTTTGTCCTAAAATGGACTTTTTTTTATCATGATTTTTATTTTGTTG

mRNA sequence

ATGCAGTTCATGAGCTCATTTTTGTCTGCCTCCGAGCAGCCTCAGATGGGGCAACAGGATTCTGTGGACAAGGAGTCTCTTACGGATACTTGCGATAGTTACATGAATAGGGATGGTGTTTCTACAAGTGGAAATATTCACATGCCAACTATAAATAATGATGAAGCTAGAAGAATGAATGAGAATAAAAGCGATAAACATAATGTTCCACATGTATATAGACGAGATATTGTAAAAAGCAAAGGCAGTGGTATGATAGGGATTGTGACTGAAGTTGCTGGTGATGCTGATTCAGATACTGACATTACTGATGATGAAGATGACTATGATGATGGTGAGGATGGTCTTGATGATGATGGTTGTGATGATTATGGGGAGAACGAAGGTCAAAACAAGGAAAACTATGACGATGACACTAATGCTAAAAATTCAAACAGAGATAGTTATAAGAATCAACCCCTTACTGCTGATGAAGTTCGAGTCCTCTGGATGGATGAAAGTGAGACTACTCAGAATGTTAATGATTTAACAGTTATAGATAGGGGTTTTTTACATGGTGATTTTGTTGCTGCTGTTTCCAATCCTACAGGGCAAGCAGGTGTTGTGGTAGATGTAAATATCTCTGTTGACTTGTTAGTTCCTGATGGATCAGTTGTGAAAAATATATCATCTAAGGACTTGATACGTGTGAGAGATTTCACGGTGGGGGATTATGTGGTCCTTGGTCCGTGGTTGGGTAGAATTGATGATATCTTGGATAATGTGACTGTGATGTTTGATGATGGCTCCAAGTGTAAAGTCACAAAGGCAGAGCCATCGCGGCTCAAACCAGTTTCTAAGAATATCCTTGAAGATGCAAGCTTTCCTTACTATCCTGGCCAGCGGGTGAGGGCGAGTTCTTCAACAGTTTTTAAGAATTCTAAATGGCTTTCTGGTTTATGGAAACCTAATCGATTGCAAGGTACTGTCACTAAAGTTACAGTTGGATCAGTGTTCATTTATTGGATAGCATCTGCTGGATATGGACCAGATTCTTCTACCACCCCAGCTGAAGAACAGAGTCCAAAGAACTTGAGACTATTAACTTGTTTTTCACATGTGAATTGGCAATTGGGTGACTGGTGTCTTCTTCCCTCCTCATTCTCCTCTGGCTCGACTAAGGACTCATCACAAACAGAACTTTCTGATTCAGTGACCAATACGTTAGATTACGCACAAACAGTGGGAGCATGCGATTCAGAGGATACTGTGCTAGATGAATTAAATGGGAGAACTGAATCCACTGATCTTGACTCCATTAGTGCATGTAATGGAAATTATAGGGATCCAGAATGTCATATTTTGCCGGAATCTAGTAGTTCTAGAGCAACAAAGGAGAATACCCATGAGACTTGGCCTCTACATCGCAAAAAAATTCGTAAAGTTGTCATCAGGAGAGATAAGAAGGCTAGAAAGAAAGATGACAATTTTGAGAGGGCCCTTTTAATCATCAATACTTCGACAAAAGTTGATGTTGTATGGCAGGATGGCCGGACAGAACTTGCGCTGGATTCAACTGGCTTGATTCCAATTAATCCAGGTGATCATGAATTTGTTCCAGAACAGTATGTGGTTGAGAAGGCCTCTGATAATGATGATGATGTTACAGAAAGCAGGCGAGTTGGAGTTGTGAAAAGTGTTCATGCAAATGAGCGAACAGCCTGTGTAAGGTGGTTAAAACCAGTCTCCAGGGCAGAGGATCCTCGAGAATTTGACATAGAAGAAGTTGTTAGTGTGTATGAGCTAGAAGGGCATCCAGATTATGACTACTGTTATGGTGATGTTGTTGTTCGTTTGTCACCTGTTTCTGATTCTATAGAAGCTCTGTCTTCTGAAAACAATACTGAAGAATTAAATCATCAAAGTTCAACCAATGGGGTGATGAGTTGTACAGAAAAGGTTTCAGGAAGCCTGAAAATAGAATATCCATCAAGTAGTGATGATTTCTTTGACTTCTCAGATCTCTCTTGGGTTGGAAATATAACGAATCTTAAGAATGGTGATATTGAAGTTTTTTGGGCCAATGGCATGGTTTCAACGGTTGGCCCTCAAGCAATTTATGTTGTTGGTCGAGATGATGATGATGAATCAATTGGTGCTGGAAGTGAAATAAGTAACGGTGCTGCAAGTTGGGAAACAATTGATGATGATGATGATGATGATGAAATGGATGTTGAGAACGCCAAGGAGGATATTGAGCTGCAAGATACTGGAGTGGATTCTGAAGAGGAAGAGAGTGAACAGCATAATTCAGGAAGAAGTCTAGCTCTTTCTGTTCCACTTGCTGCGTTTCGATTTGTTACCAGATTGGCTACTGGAATATTCTCACGAGGACCAAGAAATCGAGAATCAATGGATTTGGATTCCCACAGTGAAAGTGACACTCAATCCTTGGAAATTCAAGCTTTGGAGGAAATAGATTCTGGGCTTCAGTCTAGCTCTCTTAAATCCAATTCTTTTACTGCTTCTGATATGAACTCTGACTGTGGAAGAGGAGAGGACGATGATGCCTCAGAGCCATCAAAAGTGTTAGTATCAGCAGAAGCTTCAAGCAACCTGAGGACTGCTCAATCAGATGGGTCAACATGCCATGAGGATGGCACCTGCAGCTTCAAGGGCTTTGATATCGCTAAAGATCCTTTGGACCATTATTTTCTTGGTACGAATGGTCAAACTATTAACGGAAGGAAATGGCTTAAGAAAATCCAGCAAGATTGGACTATCCTCCAGAATAATCTTCCAGATGGGATATACGTTCAAGTCTATGAGGATCGAATGGACCTTTTAAGAGCCGTCATTGTTGGGGCCTATGGAACCCCTTACCAAGATGGCCTTTTCTTCTTCGATTTTCACCTCCCACCAGATTATCCCGACGTTCCTCCTTCAGCATATTATCATTCAGGTGGGTGGCGGATAAATCCCAATCTTTATGAGGAAGGCAAAGTGTGCCTTAGCCTTTTAAATACTTGGACTGGCAGAGGAAATGAAGTGTGGGATCCAAAGTCCTCTAGCATCCTTCAAGTGTTGGTATCGCTCCAGGGGTTAGTGTTGAATTCTAAACCATATTTTAATGAAGCGGGATATGACAAACAAATTGGGACAGCAGAAGGAGAGAAGAATTCCTTGTCGTACAATGAAAACACCTTCTTGTTGAACTGCAAGACAATAATGTATCTTATGAGGAAGCCTCCCAAGGACTTTGAAGAACTCGTTAAAGAACATTTCAGGAAGCGTGGATATTACATCCTGAAGGCCTGTGATGCATACATGAAAGGTCACTTAATTGGCTCTCTCACAGAAGATGCCTCTATAAGAGAAGAAAGCGATCCCAACTCCACGTCAGTCGGGTTTAAGCTTATGCTCGCTAAGATTGTTCCGAAGCTGTTCTCGTCGTTGAACGAAGTCAGAGCTGATTGTCGTGATTTTAAGCATTTTCAACAGTTGTAGCATTACGAAAAACTCCAGATGACAAGGTTGTCATCATTTACACATAGCTGACAAGGTAATCTTTTTCTTTTCTTTTCTTCTATTCATTCCCATTCATGTTAAAGCCTTCTTGTCTTTACCCATTATGATCAATGATGCACCTTGTTTATAAGTTTTGAACTGGGGGCCTCATGAACTTCAAAAGCTTCCTAGAATTCTAGGATCTGGGTTTTTTGTGACAATTCTTTGGTCTGTGATCCATTTTTTTCCTTAGAAAAAACGAAGGAAAGTCAAATTACATTGCAAGTTAGGAGTTCGTTGGTCTTGTTCTGTCGTAGGTTGTTTGTCCTAAAATGGACTTTTTTTTATCATGATTTTTATTTTGTTG

Coding sequence (CDS)

ATGCAGTTCATGAGCTCATTTTTGTCTGCCTCCGAGCAGCCTCAGATGGGGCAACAGGATTCTGTGGACAAGGAGTCTCTTACGGATACTTGCGATAGTTACATGAATAGGGATGGTGTTTCTACAAGTGGAAATATTCACATGCCAACTATAAATAATGATGAAGCTAGAAGAATGAATGAGAATAAAAGCGATAAACATAATGTTCCACATGTATATAGACGAGATATTGTAAAAAGCAAAGGCAGTGGTATGATAGGGATTGTGACTGAAGTTGCTGGTGATGCTGATTCAGATACTGACATTACTGATGATGAAGATGACTATGATGATGGTGAGGATGGTCTTGATGATGATGGTTGTGATGATTATGGGGAGAACGAAGGTCAAAACAAGGAAAACTATGACGATGACACTAATGCTAAAAATTCAAACAGAGATAGTTATAAGAATCAACCCCTTACTGCTGATGAAGTTCGAGTCCTCTGGATGGATGAAAGTGAGACTACTCAGAATGTTAATGATTTAACAGTTATAGATAGGGGTTTTTTACATGGTGATTTTGTTGCTGCTGTTTCCAATCCTACAGGGCAAGCAGGTGTTGTGGTAGATGTAAATATCTCTGTTGACTTGTTAGTTCCTGATGGATCAGTTGTGAAAAATATATCATCTAAGGACTTGATACGTGTGAGAGATTTCACGGTGGGGGATTATGTGGTCCTTGGTCCGTGGTTGGGTAGAATTGATGATATCTTGGATAATGTGACTGTGATGTTTGATGATGGCTCCAAGTGTAAAGTCACAAAGGCAGAGCCATCGCGGCTCAAACCAGTTTCTAAGAATATCCTTGAAGATGCAAGCTTTCCTTACTATCCTGGCCAGCGGGTGAGGGCGAGTTCTTCAACAGTTTTTAAGAATTCTAAATGGCTTTCTGGTTTATGGAAACCTAATCGATTGCAAGGTACTGTCACTAAAGTTACAGTTGGATCAGTGTTCATTTATTGGATAGCATCTGCTGGATATGGACCAGATTCTTCTACCACCCCAGCTGAAGAACAGAGTCCAAAGAACTTGAGACTATTAACTTGTTTTTCACATGTGAATTGGCAATTGGGTGACTGGTGTCTTCTTCCCTCCTCATTCTCCTCTGGCTCGACTAAGGACTCATCACAAACAGAACTTTCTGATTCAGTGACCAATACGTTAGATTACGCACAAACAGTGGGAGCATGCGATTCAGAGGATACTGTGCTAGATGAATTAAATGGGAGAACTGAATCCACTGATCTTGACTCCATTAGTGCATGTAATGGAAATTATAGGGATCCAGAATGTCATATTTTGCCGGAATCTAGTAGTTCTAGAGCAACAAAGGAGAATACCCATGAGACTTGGCCTCTACATCGCAAAAAAATTCGTAAAGTTGTCATCAGGAGAGATAAGAAGGCTAGAAAGAAAGATGACAATTTTGAGAGGGCCCTTTTAATCATCAATACTTCGACAAAAGTTGATGTTGTATGGCAGGATGGCCGGACAGAACTTGCGCTGGATTCAACTGGCTTGATTCCAATTAATCCAGGTGATCATGAATTTGTTCCAGAACAGTATGTGGTTGAGAAGGCCTCTGATAATGATGATGATGTTACAGAAAGCAGGCGAGTTGGAGTTGTGAAAAGTGTTCATGCAAATGAGCGAACAGCCTGTGTAAGGTGGTTAAAACCAGTCTCCAGGGCAGAGGATCCTCGAGAATTTGACATAGAAGAAGTTGTTAGTGTGTATGAGCTAGAAGGGCATCCAGATTATGACTACTGTTATGGTGATGTTGTTGTTCGTTTGTCACCTGTTTCTGATTCTATAGAAGCTCTGTCTTCTGAAAACAATACTGAAGAATTAAATCATCAAAGTTCAACCAATGGGGTGATGAGTTGTACAGAAAAGGTTTCAGGAAGCCTGAAAATAGAATATCCATCAAGTAGTGATGATTTCTTTGACTTCTCAGATCTCTCTTGGGTTGGAAATATAACGAATCTTAAGAATGGTGATATTGAAGTTTTTTGGGCCAATGGCATGGTTTCAACGGTTGGCCCTCAAGCAATTTATGTTGTTGGTCGAGATGATGATGATGAATCAATTGGTGCTGGAAGTGAAATAAGTAACGGTGCTGCAAGTTGGGAAACAATTGATGATGATGATGATGATGATGAAATGGATGTTGAGAACGCCAAGGAGGATATTGAGCTGCAAGATACTGGAGTGGATTCTGAAGAGGAAGAGAGTGAACAGCATAATTCAGGAAGAAGTCTAGCTCTTTCTGTTCCACTTGCTGCGTTTCGATTTGTTACCAGATTGGCTACTGGAATATTCTCACGAGGACCAAGAAATCGAGAATCAATGGATTTGGATTCCCACAGTGAAAGTGACACTCAATCCTTGGAAATTCAAGCTTTGGAGGAAATAGATTCTGGGCTTCAGTCTAGCTCTCTTAAATCCAATTCTTTTACTGCTTCTGATATGAACTCTGACTGTGGAAGAGGAGAGGACGATGATGCCTCAGAGCCATCAAAAGTGTTAGTATCAGCAGAAGCTTCAAGCAACCTGAGGACTGCTCAATCAGATGGGTCAACATGCCATGAGGATGGCACCTGCAGCTTCAAGGGCTTTGATATCGCTAAAGATCCTTTGGACCATTATTTTCTTGGTACGAATGGTCAAACTATTAACGGAAGGAAATGGCTTAAGAAAATCCAGCAAGATTGGACTATCCTCCAGAATAATCTTCCAGATGGGATATACGTTCAAGTCTATGAGGATCGAATGGACCTTTTAAGAGCCGTCATTGTTGGGGCCTATGGAACCCCTTACCAAGATGGCCTTTTCTTCTTCGATTTTCACCTCCCACCAGATTATCCCGACGTTCCTCCTTCAGCATATTATCATTCAGGTGGGTGGCGGATAAATCCCAATCTTTATGAGGAAGGCAAAGTGTGCCTTAGCCTTTTAAATACTTGGACTGGCAGAGGAAATGAAGTGTGGGATCCAAAGTCCTCTAGCATCCTTCAAGTGTTGGTATCGCTCCAGGGGTTAGTGTTGAATTCTAAACCATATTTTAATGAAGCGGGATATGACAAACAAATTGGGACAGCAGAAGGAGAGAAGAATTCCTTGTCGTACAATGAAAACACCTTCTTGTTGAACTGCAAGACAATAATGTATCTTATGAGGAAGCCTCCCAAGGACTTTGAAGAACTCGTTAAAGAACATTTCAGGAAGCGTGGATATTACATCCTGAAGGCCTGTGATGCATACATGAAAGGTCACTTAATTGGCTCTCTCACAGAAGATGCCTCTATAAGAGAAGAAAGCGATCCCAACTCCACGTCAGTCGGGTTTAAGCTTATGCTCGCTAAGATTGTTCCGAAGCTGTTCTCGTCGTTGAACGAAGTCAGAGCTGATTGTCGTGATTTTAAGCATTTTCAACAGTTGTAG

Protein sequence

MQFMSSFLSASEQPQMGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRRDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDFKHFQQL
Homology
BLAST of CmaCh02G002670 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 684/1131 (60.48%), Postives = 847/1131 (74.89%), Query Frame = 0

Query: 37   RDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRRDIVKSKGSGMIGIVTEVAGDA 96
            +D   TS N+ + +  +D    ++   S+    P++YR+DIVK+K +G +G+V+EVAGD+
Sbjct: 5    QDDPGTSTNVGVDSSVDDSMASLSICDSEH---PNIYRQDIVKNKKTGSVGVVSEVAGDS 64

Query: 97   DSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENYDDDTNAKNSNRDSYKNQPLTA 156
            DSD+DI+D+E+D DD ED  DDD  +D  E +  ++EN  +    K ++  +YK   L  
Sbjct: 65   DSDSDISDEEEDDDDDEDNDDDD--EDVEEGKKASEENVVNGDGEKKAD-GNYKCGALEG 124

Query: 157  DEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDG 216
            D++RVLWMD +E  Q++ND+TVIDRGFLHGD+VA+ S PTGQ GVVVDVNISVDLL PDG
Sbjct: 125  DQIRVLWMDNTEPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDG 184

Query: 217  SVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLK 276
            S+ K+IS+K+L RVRDF VGDYVV GPWLGRIDD+LDNVTV+FDDGS CKV + EP RLK
Sbjct: 185  SIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLK 244

Query: 277  PVSKNIL-EDASFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYW 336
            P+ KN L EDA+FPYYPGQRV+ASSS+VFKNS+WLSGLWKPNRL+GTVTKVT GS+F+YW
Sbjct: 245  PIPKNNLEEDANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYW 304

Query: 337  IASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGST---KDSSQT 396
            IASAG+GPDSS +P EEQ+P NL LL+CF+H NWQ+GDWCLLPS   S +    K  S+ 
Sbjct: 305  IASAGFGPDSSVSPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKL 364

Query: 397  ELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISACNGNYRDPECHILPESS 456
             L DS     D  Q +G  D ED V DE++G+ E   + + +       DP     P+ +
Sbjct: 365  RLYDS---QADRQQKIGR-DLED-VQDEVSGKVEPAGITAEALPKVTSDDP-----PQRN 424

Query: 457  SSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALLIINTSTKVDVVWQDGRT 516
             S  +KE  HE WPLHRKKIRK+VIR+DKK +KK+++FE+ALL++N+ T+VDV WQDG  
Sbjct: 425  PS-VSKEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTI 484

Query: 517  ELALDSTGLIPI-NPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTACVRW 576
            E   ++  LIPI  PGDHEFV EQYVVEK SD+ D+ TE RR GVVK+V+A +RTA VRW
Sbjct: 485  ECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRW 544

Query: 577  LKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNTEEL 636
            L P+ RAE+PREF+ EE+VSVYELEGHPDYDYCYGDVVVRLSP++ ++ A S  N+ EE 
Sbjct: 545  LNPLRRAEEPREFEKEEIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSFEEA 604

Query: 637  NHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLKNGDIEVFWANGMV 696
              Q   NG           + ++    ++   D S LSWVGNIT LK+GDIEV WA+G +
Sbjct: 605  TQQD--NGYQDSESHQEAKILVD-KEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTI 664

Query: 697  STVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDD-DDDEMDVENAKEDIELQDT 756
            STVGP A+YVVGRDDDDES+   SE S+ AASWET++DDD    E+  E+      ++  
Sbjct: 665  STVGPHAVYVVGRDDDDESVAGESETSD-AASWETLNDDDRGAPEIPEEDLGRSSSIEG- 724

Query: 757  GVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNRESMDLDSHSESDTQS 816
              +S+ +   +++SGR+ AL++PLAA  FVTRLA+GIFSR    R+S+D  S S+   ++
Sbjct: 725  --NSDADIYAENDSGRNGALALPLAAIEFVTRLASGIFSRA---RKSVD-SSSSDYTVEN 784

Query: 817  LEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSKVLVSAEASSNLRTAQ 876
            +  QA E  +   ++ SL   S +  ++  +C   E       +K ++S E S+ L    
Sbjct: 785  VYKQA-ESTNPSDETDSLDDPSPSKVNVTDNC---ESKGTQANAKNILSGETSTFLE--D 844

Query: 877  SDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQNNLPDGI 936
             D     E  +CSF+ FDI++DPLDH+FLG +GQ    R+W KK+ QDW ILQNNLPDGI
Sbjct: 845  EDKPVPSEGDSCSFRRFDISQDPLDHHFLGVDGQKTKERQWFKKVDQDWKILQNNLPDGI 904

Query: 937  YVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLY 996
            +V+ YEDRMDLLRAVIVGA+GTPYQDGLFFFDFHLP DYP VPPSAYYHSGGWR+NPNLY
Sbjct: 905  FVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLY 964

Query: 997  EEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEG 1056
            EEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ+GTAEG
Sbjct: 965  EEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEG 1024

Query: 1057 EKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGS 1116
            EKNSL YNENTFLLNCKT+MYLMRKPPKDFEEL+K+HFRKRGYYILKACDAYMKG+LIGS
Sbjct: 1025 EKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGS 1084

Query: 1117 LTEDAS-IREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDFKHFQQ 1161
            LT+DAS I E S  NSTSVGFKLMLAKI PKLFS+L+EV ADC +F+H QQ
Sbjct: 1085 LTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEFQHLQQ 1101

BLAST of CmaCh02G002670 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-166
Identity = 365/997 (36.61%), Postives = 531/997 (53.26%), Query Frame = 0

Query: 168  ETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDL 227
            ET   +++    +RGF++GD V + + P+GQ+G V+++++ V+L    G ++K + +K L
Sbjct: 41   ETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRL 100

Query: 228  IRVRDFTVGDYVVLGPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDAS 287
             ++R  ++ DYV+ GPW+GR+D I++ V+V  DDG+  +V      +L  +  N+LED+ 
Sbjct: 101  QKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQ 160

Query: 288  FPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIAS-AGYGPDSS 347
            + YYPGQRV+   +   +++ WL G W+  ++ GTV  V  G V++ W+AS    G  + 
Sbjct: 161  YSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNL 220

Query: 348  TTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQ 407
            T P   Q+P++L LL C SH +WQLGDWC+LP          SS  ++++  T       
Sbjct: 221  TAPQALQNPESLTLLPCVSHASWQLGDWCILPG---------SSHCDIAERQT------- 280

Query: 408  TVGACDSEDTVLDELNGRTESTDLDSISACNGNYRDPECHILPESSSSRATKENTHETWP 467
                                     +++A N N    ECH   +   +R           
Sbjct: 281  ------------------------PNVAAYNLN----ECHKTFQKGFNR----------- 340

Query: 468  LHRKKIRKVVIRRDKKARKKDDNFERALLIINTSTKVDVVWQDGRTELALDSTGLIPINP 527
                               ++   +   +I  T  KV V+WQDG   L +DS  L+P+  
Sbjct: 341  -----------------NMQNSGLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGA 400

Query: 528  -GDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTACVRWLKPVSR-AEDPREF 587
               H+F PEQ+VVEK + N      S++ GVVK+V+A E+T  V+W   V + A    + 
Sbjct: 401  VNAHDFWPEQFVVEKETCN------SKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDE 460

Query: 588  DIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNTEELNHQSSTNGVMSCT 647
             +EE+VS YEL  HPD+ +C+ DVVV+L P                        G     
Sbjct: 461  VMEEIVSAYELLEHPDFGFCFSDVVVKLLP-----------------------EGKFDPN 520

Query: 648  EKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGR 707
                 + + ++  +  D+     LS +G +T  KNG ++V WANG  S V P  I+ + R
Sbjct: 521  ADTIVATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWANGSTSKVAPCEIWKMER 580

Query: 708  DDDDESIGAGSEISNGAASWETIDDDDDDDEMDVENAKEDIELQDTGVDSEEEESEQHNS 767
             +   S    SE S    S + I   D+      E     ++L   G      ES   N 
Sbjct: 581  SEYSNSSTVSSEGSVQDLS-QKISQSDEASSNHQETGL--VKLYSVG------ESCNENI 640

Query: 768  GRSLALSVPLAAFRFVTRLATGIF----SRGPRNRESMDLDSHSESDTQSLEIQALEEID 827
                +  +P AA  F+T LA+ +F    S    +  S   DS  +SD++ L  +  E  D
Sbjct: 641  PECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQETAESYD 700

Query: 828  SGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSKVLVSAEASSNLRTAQSDGSTCHED- 887
                              NS+   GE D  +  + V +  E     +T     ST  E+ 
Sbjct: 701  ------------------NSETNSGEVDMTT--TMVNIPIEGKGINKTLD---STLLENS 760

Query: 888  -GTCSFKGFDIAKDPLDHYFLGTN---GQTINGRKWLKKIQQDWTILQNNLPDGIYVQVY 947
                 F+ FD+  D  DH+FL ++    Q+   + W+KK+QQ+W+ L+ NLP+ IYV+V 
Sbjct: 761  RNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVC 820

Query: 948  EDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKV 1007
            E+RMDLLRA +VGA GTPY DGLFFFD  LPP YP  PP  +YHSGG R+NPNLYE G+V
Sbjct: 821  EERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRV 880

Query: 1008 CLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSL 1067
            CLSLLNTW+G G EVW+  SSSILQ+L+S Q LVLN KPYFNEAGYDKQ+G AEGEKNS+
Sbjct: 881  CLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSV 899

Query: 1068 SYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDA 1127
            SYNEN FL+ CK+++ ++RKPPK FE LVK+HF  R  ++L AC AYM+G  +GS    A
Sbjct: 941  SYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS---SA 899

Query: 1128 SIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADC 1153
            +++  S  NST  GFK+ML+K+ PKL  + +E+  DC
Sbjct: 1001 NLQGNSTTNST--GFKIMLSKLYPKLLEAFSEIGVDC 899

BLAST of CmaCh02G002670 vs. ExPASy Swiss-Prot
Match: Q9C0C9 ((E3-independent) E2 ubiquitin-conjugating enzyme OS=Homo sapiens OX=9606 GN=UBE2O PE=1 SV=3)

HSP 1 Score: 292.7 bits (748), Expect = 1.8e-77
Identity = 293/1145 (25.59%), Postives = 492/1145 (42.97%), Query Frame = 0

Query: 122  DDYGENEGQNKENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVND--LTVI 181
            D  GE EG+      +   A  +  +  +  PL    VRV W  E    Q+V +  L + 
Sbjct: 88   DSEGEEEGRGSSGCSE---AGGAGHEEGRASPLRRGYVRVQWYPEG-VKQHVKETKLKLE 147

Query: 182  DRGFLHGDFVAAVSNPTGQAGVVVDVNISVDL-LVPDGSVVKNISSKDLIRVRDFTVGDY 241
            DR  +  D V  + +   Q G V+DVNI   + L+    ++  ++SKDL  +  F  GDY
Sbjct: 148  DRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDY 207

Query: 242  VVLGPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILE-----DASFPYYPG 301
            +    WLG++ D+ + + +   +G++C +   + ++L  V  ++ +     D S+ +YPG
Sbjct: 208  IAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPG 267

Query: 302  QRVRASSSTVFKNSKWLSGLWKP-----NRLQGTVTKVTVGSVFIYWIASA--GYGPDSS 361
            Q V    + +F + +WLSG+ KP     ++ +  V +V V  + + WI  +    G DS 
Sbjct: 268  Q-VLIGPAKIFSSVQWLSGV-KPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSV 327

Query: 362  TTPAEEQSPKNL---RLLTCFSHVNWQLGDWCLL-------PSSFS-----------SGS 421
            + P    + +NL   + L CF H   QLG+ CL        P+  +            GS
Sbjct: 328  SPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGS 387

Query: 422  TKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLD-----ELNGRTESTDLDSISACNGNY 481
                 +  L   V   +  +     C  + ++ D     E   ++E+         +G+ 
Sbjct: 388  MAKKVKRLLKKQVVRIMSCSPDT-QCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSA 447

Query: 482  ----------------------------RDPECHILPE----------------SSSSRA 541
                                        RD   H   +                +SS+ +
Sbjct: 448  SPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASS 507

Query: 542  TKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFER--------ALLIINTSTKVDVVWQ 601
            T  +   +    +K I   +    +K ++K +   R        A+ ++ T T  DV+WQ
Sbjct: 508  TTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQ 567

Query: 602  DGRTELALDSTGLIPINPGD-HEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTA 661
            DG  E  + S  L P++  D +EF P  +VV+K   +  D       GVV+S     RT 
Sbjct: 568  DGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAV---YGVVQSGDHIGRTC 627

Query: 662  CVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENN 721
             V+W K     +D      EE VSVY++  HPD+ +   D+V+R+              N
Sbjct: 628  MVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRI-------------GN 687

Query: 722  TEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLK-NGDIEVFW 781
            TE+                     K + PS             VG +  +  +  +EV W
Sbjct: 688  TED-----------------GAPHKEDEPS-------------VGQVARVDVSSKVEVVW 747

Query: 782  ANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDD---------DDDEMD 841
            A+   + + PQ +Y +  + ++    +    ++GA+S E  DD D         +D+   
Sbjct: 748  ADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPK 807

Query: 842  VEN-----AKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLA-------------AFR- 901
            +E       ++ +  +D GV   EE +     G ++A++ P+A             +FR 
Sbjct: 808  IEEPPIPPLEQPVAPEDKGVVISEEAATAAVQG-AVAMAAPMAGLMEKAGKDGPPKSFRE 867

Query: 902  --------------FVTRLATGIFSRGPRNRESMDLDSHSESDTQSLEIQALEEIDSGLQ 961
                           V +L TG  +      E    +     D + L+    + +D+   
Sbjct: 868  LKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAI 927

Query: 962  SSSLKSNSFTASDMNSDCGRGEDDDASE-PSKVLVSAEASSNLRTAQSDGSTCHEDGTCS 1021
                K  +    +   D   G+    +E PS+  V  +     +     G T        
Sbjct: 928  VEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQ-----QCGGKPGVTFTSAKGEV 987

Query: 1022 FKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLR 1081
            F   + A  P +H F     Q    +K+   ++++  +L  +LP+GI V+ +EDRMDL  
Sbjct: 988  FSVLEFA--PSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFS 1047

Query: 1082 AVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS-AYYHSGGWRINPNLYEEGKVCLSLLNT 1128
            A+I G   TPY+DGL+ FD  LP  YP VPP   Y      R+NPNLY+ GKVC+SLL T
Sbjct: 1048 ALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGT 1107

BLAST of CmaCh02G002670 vs. ExPASy Swiss-Prot
Match: Q6ZPJ3 ((E3-independent) E2 ubiquitin-conjugating enzyme UBE2O OS=Mus musculus OX=10090 GN=Ube2o PE=1 SV=3)

HSP 1 Score: 287.7 bits (735), Expect = 5.7e-76
Identity = 292/1170 (24.96%), Postives = 498/1170 (42.56%), Query Frame = 0

Query: 72   VYRRDIV--KSKGSGMIGIVTEVAG-DADSDTDITDDEDDYDDGEDGLDDDGCDDYGENE 131
            ++  D+V  + +GS   G+V  + G D+DS+ D           +DG    GC + G   
Sbjct: 55   LFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGD-----------DDGRGSSGCSEAG--- 114

Query: 132  GQNKENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVND--LTVIDRGFLHG 191
                           +  +  +  PL    VRV W  E    Q+V +  L + DR  +  
Sbjct: 115  --------------GAGHEEGRASPLRRGYVRVQWYPEG-VKQHVKETKLKLEDRSVVPR 174

Query: 192  DFVAAVSNPTGQAGVVVDVNISVDL-LVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWL 251
            D V  + +   Q G V+DVNI   + L+    ++  ++SKDL  +  F  GDY+    WL
Sbjct: 175  DVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWL 234

Query: 252  GRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILE-----DASFPYYPGQRVRASS 311
            G++ D+ + + +   +G++C +   + ++L  V  ++ +     D S+ +YPGQ V    
Sbjct: 235  GKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQ-VLIGP 294

Query: 312  STVFKNSKWLSGLWKP-----NRLQGTVTKVTVGSVFIYWIASA--GYGPDSSTTPAEEQ 371
            + +F + +WLSG+ KP     ++ +  V +V V  + + WI  +    G DS + P    
Sbjct: 295  AKIFSSVQWLSGV-KPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSII 354

Query: 372  SPKNL---RLLTCFSHVNWQLGDWCL---------------------------------- 431
            + +NL   + L CF H   QLG+ CL                                  
Sbjct: 355  TQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKR 414

Query: 432  -----------------LPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGAC---DSEDT 491
                              P   S        +      + +     Q  G+    + +D 
Sbjct: 415  LLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQDE 474

Query: 492  VLDELNGRTESTDL--------DSISACNGNYRDPECHILPESSSSRATKENT------- 551
              +EL    E            D + +   +  D       ++SS  ++  +T       
Sbjct: 475  GSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGS 534

Query: 552  ----HETWPLHRKKIRKVVIRRDKKARKKDDNFER-ALLIINTSTKVDVVWQDGRTELAL 611
                 ++ PL  K +++   R+  K  +     +R A+ ++ T T  DV+WQDG  E  +
Sbjct: 535  GTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNI 594

Query: 612  DSTGLIPINPGD-HEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTACVRWLKPV 671
             S  L P++  D +EF P  +VV+K   +  D       GVV+S     RT  V+W K  
Sbjct: 595  RSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAV---YGVVQSGDHVGRTCMVKWFKLR 654

Query: 672  SRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNTEELNHQS 731
               +D      EE VSVY++  HPD+ +   D+V+R+              NTE+     
Sbjct: 655  PSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRI-------------GNTED----- 714

Query: 732  STNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLK-NGDIEVFWANGMVSTV 791
                         G+L  E            D   VG +  +  +  +EV WA+   + +
Sbjct: 715  -------------GALPKE------------DEPSVGQVARVDVSSKVEVVWADNSKTII 774

Query: 792  GPQAIYVVGRDDDDESIGAGSEISNGAAS--WETIDDDDDDDEMDVENAKEDIELQDTGV 851
             PQ +Y +  + ++    +    S+GA+S  WE   D  + D   V++    IE     +
Sbjct: 775  LPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAIL 834

Query: 852  DSEEEESEQHNSGRSLALSVPLAAFRFVTRLA---TGIFSRGPRNRESMDLDSHSES--D 911
             +E+  + + + G  ++     AA +    +A    G+  +  ++          E+   
Sbjct: 835  PAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKI 894

Query: 912  TQSLEIQALEEIDSGLQSS-------------------SLKSNSFTASDMNSDCGRGEDD 971
             +SL+   +E++ +G  +S                    L+ N     D  +     + +
Sbjct: 895  LESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKME 954

Query: 972  DASEPSKVLVSAEASSNLRTAQSDGS--TCHEDGTCSFKGFDIAKD---------PLDHY 1031
               +  +     E  S ++      +   C + G      F  AK          P +H 
Sbjct: 955  AVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHS 1014

Query: 1032 FLGTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDG 1091
            F     Q    +K+   ++++  +L  +LPDGI V+ +EDRMDL  A+I G   TPY+DG
Sbjct: 1015 FKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDG 1074

Query: 1092 LFFFDFHLPPDYPDVPPS-AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSS 1107
            L+ FD  LP  YP VPP   Y      R+NPNLY+ GKVC+SLL TW G+G E W  K S
Sbjct: 1075 LYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSK-S 1134

BLAST of CmaCh02G002670 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 4.9e-75
Identity = 135/257 (52.53%), Postives = 176/257 (68.48%), Query Frame = 0

Query: 884  FKGFDIAKDPLDHYFL--GTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDL 943
            FK FDI +D  DH++   GT  +  + + W K+IQ +W IL+ +LP+ I+V+ YE RMDL
Sbjct: 305  FKRFDIVEDFSDHHYASKGTTSKQ-HSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDL 364

Query: 944  LRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 1003
            LRAVI+GA GTPY DGLFFFD   P  YP  PP  +YHSGG RINPNLY  GKVCLSLL 
Sbjct: 365  LRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLG 424

Query: 1004 TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1063
            TW+G   E W P +S++LQVLVS+QGL+LN KPYFNE GY++  G+A GE  S +Y+ENT
Sbjct: 425  TWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENT 484

Query: 1064 FLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDASIREES 1123
            F+L+ KT++Y MR+PPK FE+    HF    + +LKAC+AY  G   G L + A   EE+
Sbjct: 485  FILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEEN 544

Query: 1124 DPNSTSVGFKLMLAKIV 1139
                +S+ F+  +A  V
Sbjct: 545  SAGMSSLKFRTDVATFV 560

BLAST of CmaCh02G002670 vs. ExPASy TrEMBL
Match: A0A6J1I476 (probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita maxima OX=3661 GN=LOC111469743 PE=4 SV=1)

HSP 1 Score: 2275.4 bits (5895), Expect = 0.0e+00
Identity = 1146/1146 (100.00%), Postives = 1146/1146 (100.00%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR
Sbjct: 1    MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENY 135
            DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENY
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENY 120

Query: 136  DDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNP 195
            DDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNP
Sbjct: 121  DDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNP 180

Query: 196  TGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNV 255
            TGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNV
Sbjct: 181  TGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNV 240

Query: 256  TVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWK 315
            TVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWK
Sbjct: 241  TVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWK 300

Query: 316  PNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWC 375
            PNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWC
Sbjct: 301  PNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWC 360

Query: 376  LLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISA 435
            LLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISA
Sbjct: 361  LLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISA 420

Query: 436  CNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALL 495
            CNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALL
Sbjct: 421  CNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALL 480

Query: 496  IINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVG 555
            IINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVG
Sbjct: 481  IINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVG 540

Query: 556  VVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPV 615
            VVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPV
Sbjct: 541  VVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPV 600

Query: 616  SDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNIT 675
            SDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNIT
Sbjct: 601  SDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNIT 660

Query: 676  NLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDE 735
            NLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDE
Sbjct: 661  NLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDE 720

Query: 736  MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNR 795
            MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNR
Sbjct: 721  MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNR 780

Query: 796  ESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSK 855
            ESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSK
Sbjct: 781  ESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSK 840

Query: 856  VLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKI 915
            VLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKI
Sbjct: 841  VLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKI 900

Query: 916  QQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS 975
            QQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS
Sbjct: 901  QQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS 960

Query: 976  AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPY 1035
            AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPY
Sbjct: 961  AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPY 1020

Query: 1036 FNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYI 1095
            FNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYI
Sbjct: 1021 FNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYI 1080

Query: 1096 LKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDF 1155
            LKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDF
Sbjct: 1081 LKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDF 1140

Query: 1156 KHFQQL 1162
            KHFQQL
Sbjct: 1141 KHFQQL 1146

BLAST of CmaCh02G002670 vs. ExPASy TrEMBL
Match: A0A6J1G6I7 (probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC111451318 PE=4 SV=1)

HSP 1 Score: 2209.5 bits (5724), Expect = 0.0e+00
Identity = 1113/1149 (96.87%), Postives = 1131/1149 (98.43%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            MGQQDSVD+ESLTD CDSYMNRDGVSTSGNIHMPTIN+DEARRMNEN+SDKHN+PHVYRR
Sbjct: 1    MGQQDSVDEESLTDACDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY---GENEGQNK 135
            DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY   GENEGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYDGDGENEGQNK 120

Query: 136  ENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 195
            ENYDDDTNAKNSNRDSYKNQPL ADEVRVLWMDESETTQNVNDLTV DRGFLHGDFVAAV
Sbjct: 121  ENYDDDTNAKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVTDRGFLHGDFVAAV 180

Query: 196  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDIL 255
            SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDF VGDYVVLGPWLGRIDD+L
Sbjct: 181  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 256  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 315
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 300

Query: 316  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 375
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 376  DWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDS 435
            DWCLLPSSFSSGSTKD SQTELSDSVTNTLDYAQTVGACDSEDTV+DEL+GRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 420

Query: 436  ISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFER 495
            ISACNGNYRDPEC+ILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKK++NFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 496  ALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 555
            ALLIINT+TKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA+DNDDDVTESR
Sbjct: 481  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 540

Query: 556  RVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 615
            RVGVVKSVHA ERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 616  SPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVG 675
            SPVSDSIEALSSENNTEELN QSSTNGVMSC EKVSGSLKIEYPSSSDD FDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 660

Query: 676  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDD 735
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWET+  DDD
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETV--DDD 720

Query: 736  DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 795
            DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP
Sbjct: 721  DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 780

Query: 796  RNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASE 855
            RNRESMDLDSHSESDTQSLEIQ+LEEIDSGLQSSSLKSNSFT SDMNSDCGRGEDDDASE
Sbjct: 781  RNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASE 840

Query: 856  PSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 915
            PSK+LVSAEASSNLRTAQSDGS CHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL
Sbjct: 841  PSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 900

Query: 916  KKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDV 975
            KKIQQDWTILQNNLPDGIYV+VYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPP+YPDV
Sbjct: 901  KKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960

Query: 976  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1035
            PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS
Sbjct: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1020

Query: 1036 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRG 1095
            KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR+RG
Sbjct: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRG 1080

Query: 1096 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADC 1155
            YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEV ADC
Sbjct: 1081 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1140

Query: 1156 RDFKHFQQL 1162
            RDFKHFQQL
Sbjct: 1141 RDFKHFQQL 1147

BLAST of CmaCh02G002670 vs. ExPASy TrEMBL
Match: A0A6J1JD25 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483941 PE=4 SV=1)

HSP 1 Score: 1972.6 bits (5109), Expect = 0.0e+00
Identity = 1003/1150 (87.22%), Postives = 1063/1150 (92.43%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            M QQ SVDKESL  T +SY NRDGVS+S N+HM TINNDE RR NEN SDK +VPH+YR+
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTTINNDETRRTNENTSDKPSVPHIYRQ 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDD---YGENEGQNK 135
            DIVKSKGSGMIGIVTEVAGDADSD+DITDDED+ +DGEDG D  GCDD    GE EGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDGEDGEDGGGCDDDDGDGEKEGQNK 120

Query: 136  ENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 195
            ENY DD+NAKNSNRD+YK+QPL  DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  ENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRGFLHGDFVAAV 180

Query: 196  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDIL 255
            S+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVRDFTVGDYVVLGPWLGRIDD+L
Sbjct: 181  SDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDDVL 240

Query: 256  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 315
            DNVTVMFDDGSKCKVTKAEP RLKPVSKNILEDA+FPYYPGQRV+ASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWLSG 300

Query: 316  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 375
            LWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNLRLLTCFSH NWQLG
Sbjct: 301  LWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQLG 360

Query: 376  DWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDS 435
            DWCLLPSSFSSG TK+SSQT  SDSVTN+LD  Q    CDSED VL+EL+G TESTDLDS
Sbjct: 361  DWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELSGTTESTDLDS 420

Query: 436  ISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFER 495
            +SAC+GNYR+PEC+ILPESS+SRA KE +HETWPLHRKKIRKVVIRRDKKARKK++NFER
Sbjct: 421  LSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 496  ALLIINTSTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKASDNDDDVTES 555
            ALLIINT TKVDV WQDGRTE  LDST LIPI NPGDHEFVPEQYVVEKASDNDDD++ES
Sbjct: 481  ALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDISES 540

Query: 556  RRVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 615
            RRVGVVKSVHA ERTACV WLKPVSRAEDPREFDI+E+VSVYELEGHPDYDYCYGDVVVR
Sbjct: 541  RRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVVVR 600

Query: 616  LSPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWV 675
            LSPVSDSIE++S  NNTEEL  QSSTN +M+CTEK SGS KIE PS SDD  DFSDLSWV
Sbjct: 601  LSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLSWV 660

Query: 676  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDD 735
            GNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDESI AGSE+SNGAASWET+DDD+
Sbjct: 661  GNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDDDE 720

Query: 736  DDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRG 795
             D    V+NAKEDIELQD GV+SEEE SEQ+NSGR+LALSVP AA RF TRLA GIFSRG
Sbjct: 721  RD---SVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFSRG 780

Query: 796  PRNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDAS 855
            PRN E MD+DSHSES+TQSLEIQALEE DSGLQSSSLKSNSF ASDMNSDCGRGED+ AS
Sbjct: 781  PRNPELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSDCGRGEDNIAS 840

Query: 856  EPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKW 915
            EPS+VL SA+ASSNLRTA+SDGS CHEDGTCSFKGFDIAKDPLDHYFLGTNGQT +GRKW
Sbjct: 841  EPSEVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNSGRKW 900

Query: 916  LKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPD 975
            LKKIQQDW+ILQNNLPDGIYV+VYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPP+YPD
Sbjct: 901  LKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPD 960

Query: 976  VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN 1035
            VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLVLN
Sbjct: 961  VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLVLN 1020

Query: 1036 SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKR 1095
            SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR+R
Sbjct: 1021 SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRR 1080

Query: 1096 GYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRAD 1155
            GY+ILKACDAYMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEV AD
Sbjct: 1081 GYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLMSSLNEVGAD 1140

Query: 1156 CRDFKHFQQL 1162
            C DFKHFQQL
Sbjct: 1141 CDDFKHFQQL 1147

BLAST of CmaCh02G002670 vs. ExPASy TrEMBL
Match: A0A6J1JF34 (probable ubiquitin-conjugating enzyme E2 23 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483941 PE=4 SV=1)

HSP 1 Score: 1964.5 bits (5088), Expect = 0.0e+00
Identity = 1003/1160 (86.47%), Postives = 1063/1160 (91.64%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVS----------TSGNIHMPTINNDEARRMNENKSD 75
            M QQ SVDKESL  T +SY NRDGVS          +S N+HM TINNDE RR NEN SD
Sbjct: 1    MEQQGSVDKESLIGTGNSYTNRDGVSSSENVHMTITSSENVHMTTINNDETRRTNENTSD 60

Query: 76   KHNVPHVYRRDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDD-- 135
            K +VPH+YR+DIVKSKGSGMIGIVTEVAGDADSD+DITDDED+ +DGEDG D  GCDD  
Sbjct: 61   KPSVPHIYRQDIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDGEDGEDGGGCDDDD 120

Query: 136  -YGENEGQNKENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRG 195
              GE EGQNKENY DD+NAKNSNRD+YK+QPL  DEVRVLWMDESETT NVNDLTVIDRG
Sbjct: 121  GDGEKEGQNKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTLNVNDLTVIDRG 180

Query: 196  FLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLG 255
            FLHGDFVAAVS+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVRDFTVGDYVVLG
Sbjct: 181  FLHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLG 240

Query: 256  PWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSST 315
            PWLGRIDD+LDNVTVMFDDGSKCKVTKAEP RLKPVSKNILEDA+FPYYPGQRV+ASSST
Sbjct: 241  PWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDANFPYYPGQRVKASSST 300

Query: 316  VFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLT 375
            VFKNSKWLSGLWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNLRLLT
Sbjct: 301  VFKNSKWLSGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLT 360

Query: 376  CFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELN 435
            CFSH NWQLGDWCLLPSSFSSG TK+SSQT  SDSVTN+LD  Q    CDSED VL+EL+
Sbjct: 361  CFSHANWQLGDWCLLPSSFSSGLTKESSQTHRSDSVTNSLDSTQPEVVCDSEDVVLNELS 420

Query: 436  GRTESTDLDSISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKK 495
            G TESTDLDS+SAC+GNYR+PEC+ILPESS+SRA KE +HETWPLHRKKIRKVVIRRDKK
Sbjct: 421  GTTESTDLDSLSACDGNYRNPECNILPESSNSRALKETSHETWPLHRKKIRKVVIRRDKK 480

Query: 496  ARKKDDNFERALLIINTSTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKA 555
            ARKK++NFERALLIINT TKVDV WQDGRTE  LDST LIPI NPGDHEFVPEQYVVEKA
Sbjct: 481  ARKKEENFERALLIINTKTKVDVAWQDGRTECGLDSTTLIPIDNPGDHEFVPEQYVVEKA 540

Query: 556  SDNDDDVTESRRVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDY 615
            SDNDDD++ESRRVGVVKSVHA ERTACV WLKPVSRAEDPREFDI+E+VSVYELEGHPDY
Sbjct: 541  SDNDDDISESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDY 600

Query: 616  DYCYGDVVVRLSPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDD 675
            DYCYGDVVVRLSPVSDSIE++S  NNTEEL  QSSTN +M+CTEK SGS KIE PS SDD
Sbjct: 601  DYCYGDVVVRLSPVSDSIESISLGNNTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDD 660

Query: 676  FFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGA 735
              DFSDLSWVGNIT LKNGDIEV WANGMVSTVGPQAIYVVGRDDDDESI AGSE+SNGA
Sbjct: 661  CIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAGSEVSNGA 720

Query: 736  ASWETIDDDDDDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVT 795
            ASWET+DDD+ D    V+NAKEDIELQD GV+SEEE SEQ+NSGR+LALSVP AA RF T
Sbjct: 721  ASWETVDDDERD---SVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFT 780

Query: 796  RLATGIFSRGPRNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSD 855
            RLA GIFSRGPRN E MD+DSHSES+TQSLEIQALEE DSGLQSSSLKSNSF ASDMNSD
Sbjct: 781  RLAAGIFSRGPRNPELMDVDSHSESETQSLEIQALEEKDSGLQSSSLKSNSFDASDMNSD 840

Query: 856  CGRGEDDDASEPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGT 915
            CGRGED+ ASEPS+VL SA+ASSNLRTA+SDGS CHEDGTCSFKGFDIAKDPLDHYFLGT
Sbjct: 841  CGRGEDNIASEPSEVLESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGT 900

Query: 916  NGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFF 975
            NGQT +GRKWLKKIQQDW+ILQNNLPDGIYV+VYEDRMDLLRAVIVGAYGTPYQDGLFFF
Sbjct: 901  NGQTNSGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFF 960

Query: 976  DFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQV 1035
            DFHLPP+YPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQV
Sbjct: 961  DFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQV 1020

Query: 1036 LVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFE 1095
            LVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFE
Sbjct: 1021 LVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFE 1080

Query: 1096 ELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKL 1155
            ELVKEHFR+RGY+ILKACDAYMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL
Sbjct: 1081 ELVKEHFRRRGYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKL 1140

Query: 1156 FSSLNEVRADCRDFKHFQQL 1162
             SSLNEV ADC DFKHFQQL
Sbjct: 1141 MSSLNEVGADCDDFKHFQQL 1157

BLAST of CmaCh02G002670 vs. ExPASy TrEMBL
Match: A0A6J1FVD7 (probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC111448445 PE=4 SV=1)

HSP 1 Score: 1962.2 bits (5082), Expect = 0.0e+00
Identity = 999/1152 (86.72%), Postives = 1061/1152 (92.10%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            M QQ SVDKESL  T +SYM+RDGVS+S N+HM TINNDE R+ NEN SDK ++PH+YR+
Sbjct: 1    MEQQGSVDKESLIGTGNSYMDRDGVSSSENVHMTTINNDETRKTNENTSDKPSIPHIYRQ 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITD--DEDDYDDGEDGLDDDGCDD---YGENEGQ 135
            DIVKSKGSGMIGIVTEVAGDADSD+DITD  DED+ +DG DG DDDGCDD    GE  GQ
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDSDITDDEDEDEDEDGGDGEDDDGCDDDDGDGEKVGQ 120

Query: 136  NKENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVA 195
            NKENY DD+NAKNSNRD+YK+QPL  DEVRVLWMDESETT NVNDLTVIDRGFLHGDFVA
Sbjct: 121  NKENYGDDSNAKNSNRDNYKSQPLPDDEVRVLWMDESETTPNVNDLTVIDRGFLHGDFVA 180

Query: 196  AVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDD 255
            AVS+PTGQAGVVVDVNISVDLLVPDGS++K+ISSKDL RVRDFTVGDYVVLGPWLGRIDD
Sbjct: 181  AVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTVGDYVVLGPWLGRIDD 240

Query: 256  ILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWL 315
            +LDNVTVMFDDGSKCKVTKAEP RLKPVSKNILEDA+FPYYPGQRV+ASSSTVFKNSKWL
Sbjct: 241  VLDNVTVMFDDGSKCKVTKAEPMRLKPVSKNILEDANFPYYPGQRVKASSSTVFKNSKWL 300

Query: 316  SGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQ 375
            SGLWKPNRL+GTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQ+PKNLRLLTCFSH NWQ
Sbjct: 301  SGLWKPNRLEGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQTPKNLRLLTCFSHANWQ 360

Query: 376  LGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDL 435
            LGDWCLLPSSFSSG TK+SS+T  SDSV N+LD  Q  G CDSED VL+EL+G TESTDL
Sbjct: 361  LGDWCLLPSSFSSGLTKESSRTHRSDSVNNSLDSTQPEGVCDSEDVVLNELSGTTESTDL 420

Query: 436  DSISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNF 495
            DS+SAC+GNYR PEC+ILPESS+SRA KE THETWPLHRKKIRKVVIRRDKKARKK++NF
Sbjct: 421  DSLSACDGNYRYPECNILPESSNSRALKETTHETWPLHRKKIRKVVIRRDKKARKKEENF 480

Query: 496  ERALLIINTSTKVDVVWQDGRTELALDSTGLIPI-NPGDHEFVPEQYVVEKASDNDDDVT 555
            ERALLIINT TKVDV WQDGRTE  LDST LIPI NPGDHEFVPEQYVVEKASDNDDD++
Sbjct: 481  ERALLIINTKTKVDVAWQDGRTERGLDSTTLIPIDNPGDHEFVPEQYVVEKASDNDDDIS 540

Query: 556  ESRRVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVV 615
            ESRRVGVVKSVHA ERTACV WLKPVSRAEDPREFDI+E+VSVYELEGHPDYDYCYGDVV
Sbjct: 541  ESRRVGVVKSVHAKERTACVMWLKPVSRAEDPREFDIQEIVSVYELEGHPDYDYCYGDVV 600

Query: 616  VRLSPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLS 675
            VRLSPVSDSIE++S  N+TEEL  QSSTN +M+CTEK SGS KIE PS SDD  DFSDLS
Sbjct: 601  VRLSPVSDSIESMSLGNDTEELKQQSSTNEMMNCTEKASGSQKIEDPSCSDDCIDFSDLS 660

Query: 676  WVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDD 735
            WVGNIT LKNGDIEV WANG VSTVGPQAIYVVGRDDDDESI AGSE+SNGAASWET+DD
Sbjct: 661  WVGNITGLKNGDIEVTWANGXVSTVGPQAIYVVGRDDDDESIAAGSEVSNGAASWETVDD 720

Query: 736  DDDDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFS 795
            D+ D    V+NAKEDIELQD GV+SEEE SEQ+NSGR+LALSVP AA RF TRLA GIFS
Sbjct: 721  DERD---SVDNAKEDIELQDPGVNSEEEGSEQNNSGRNLALSVPFAAIRFFTRLAAGIFS 780

Query: 796  RGPRNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDD 855
            RGPRN ESMD+DSHSES+TQSLEIQALEE DSGL SSSLKSNSF ASDMNSDCGRGED+ 
Sbjct: 781  RGPRNPESMDVDSHSESETQSLEIQALEEKDSGLHSSSLKSNSFDASDMNSDCGRGEDNI 840

Query: 856  ASEPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGR 915
            ASEPS+V  SA+ASSNLRTA+SDGS CHEDGTCSFKGFDIAKDPLDHYFLGTNGQT NGR
Sbjct: 841  ASEPSEVSESADASSNLRTAESDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGR 900

Query: 916  KWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDY 975
            KWLKKIQQDW+ILQNNLPDGIYV+VYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPP+Y
Sbjct: 901  KWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEY 960

Query: 976  PDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLV 1035
            PDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWD KSSSILQVLVSLQGLV
Sbjct: 961  PDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDSKSSSILQVLVSLQGLV 1020

Query: 1036 LNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR 1095
            LNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR
Sbjct: 1021 LNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR 1080

Query: 1096 KRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVR 1155
            +RGY+ILKACDAYMKGHLIGSLTEDASIRE SDPNSTSVGFKLMLAKIVPKL SSLNEV 
Sbjct: 1081 RRGYFILKACDAYMKGHLIGSLTEDASIRENSDPNSTSVGFKLMLAKIVPKLVSSLNEVG 1140

Query: 1156 ADCRDFKHFQQL 1162
            ADC DFKHFQQL
Sbjct: 1141 ADCDDFKHFQQL 1149

BLAST of CmaCh02G002670 vs. NCBI nr
Match: XP_022970910.1 (probable ubiquitin-conjugating enzyme E2 23 [Cucurbita maxima])

HSP 1 Score: 2275.4 bits (5895), Expect = 0.0e+00
Identity = 1146/1146 (100.00%), Postives = 1146/1146 (100.00%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR
Sbjct: 1    MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENY 135
            DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENY
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENY 120

Query: 136  DDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNP 195
            DDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNP
Sbjct: 121  DDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNP 180

Query: 196  TGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNV 255
            TGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNV
Sbjct: 181  TGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNV 240

Query: 256  TVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWK 315
            TVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWK
Sbjct: 241  TVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSGLWK 300

Query: 316  PNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWC 375
            PNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWC
Sbjct: 301  PNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWC 360

Query: 376  LLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISA 435
            LLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISA
Sbjct: 361  LLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISA 420

Query: 436  CNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALL 495
            CNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALL
Sbjct: 421  CNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALL 480

Query: 496  IINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVG 555
            IINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVG
Sbjct: 481  IINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESRRVG 540

Query: 556  VVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPV 615
            VVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPV
Sbjct: 541  VVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPV 600

Query: 616  SDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNIT 675
            SDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNIT
Sbjct: 601  SDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNIT 660

Query: 676  NLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDE 735
            NLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDE
Sbjct: 661  NLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDDDDE 720

Query: 736  MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNR 795
            MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNR
Sbjct: 721  MDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNR 780

Query: 796  ESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSK 855
            ESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSK
Sbjct: 781  ESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSK 840

Query: 856  VLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKI 915
            VLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKI
Sbjct: 841  VLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKI 900

Query: 916  QQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS 975
            QQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS
Sbjct: 901  QQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPS 960

Query: 976  AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPY 1035
            AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPY
Sbjct: 961  AYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPY 1020

Query: 1036 FNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYI 1095
            FNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYI
Sbjct: 1021 FNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYI 1080

Query: 1096 LKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDF 1155
            LKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDF
Sbjct: 1081 LKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDF 1140

Query: 1156 KHFQQL 1162
            KHFQQL
Sbjct: 1141 KHFQQL 1146

BLAST of CmaCh02G002670 vs. NCBI nr
Match: KAG6604947.1 (putative ubiquitin-conjugating enzyme E2 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2213.0 bits (5733), Expect = 0.0e+00
Identity = 1119/1160 (96.47%), Postives = 1138/1160 (98.10%), Query Frame = 0

Query: 5    SSFLSASEQPQMGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKS 64
            SSFLSAS+QPQMGQQDSVDKESLTDTC SYMNRDGVSTSGNIHMPTIN+DEARRMNENKS
Sbjct: 16   SSFLSASDQPQMGQQDSVDKESLTDTCGSYMNRDGVSTSGNIHMPTINSDEARRMNENKS 75

Query: 65   DKHNVPHVYRRDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY 124
            DKHN+PHVYRRDIVKSKGSGMIGIVTEVAGDADSDTDITDDED YDDGEDGLDDDGCDDY
Sbjct: 76   DKHNIPHVYRRDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDVYDDGEDGLDDDGCDDY 135

Query: 125  ---GENEGQNKENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDR 184
               GENEGQNKENYDDDTNAKNSNRDSYKNQPL ADEVRVLWMDESETTQNVNDLTVIDR
Sbjct: 136  DGDGENEGQNKENYDDDTNAKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVIDR 195

Query: 185  GFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVL 244
            GFLHGDFVAAVSNPTGQ+GVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDF VGDYVVL
Sbjct: 196  GFLHGDFVAAVSNPTGQSGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVL 255

Query: 245  GPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSS 304
            GPWLGRIDD+LDNVTVMFDDGSKCKVTKAEP RLKPVSKNILEDASFPYYPGQRVRASSS
Sbjct: 256  GPWLGRIDDVLDNVTVMFDDGSKCKVTKAEPLRLKPVSKNILEDASFPYYPGQRVRASSS 315

Query: 305  TVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLL 364
            TVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLL
Sbjct: 316  TVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLL 375

Query: 365  TCFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDEL 424
            TCFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTV+DEL
Sbjct: 376  TCFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVVDEL 435

Query: 425  NGRTESTDLDSISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDK 484
            +GRTESTDLDSISACNGNYRDPEC+ILPESSSSR TKENTHETWPLHRKKIRKVVIRRDK
Sbjct: 436  SGRTESTDLDSISACNGNYRDPECNILPESSSSRTTKENTHETWPLHRKKIRKVVIRRDK 495

Query: 485  KARKKDDNFERALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA 544
            KARKK++NFERALLIINT+TKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA
Sbjct: 496  KARKKEENFERALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA 555

Query: 545  SDNDDDVTESRRVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDY 604
            SDNDDDVTESRRVGVVKSVHA ERTACVRWLKPV RAEDPREFDIEEVVSVYELEGHPDY
Sbjct: 556  SDNDDDVTESRRVGVVKSVHAKERTACVRWLKPVCRAEDPREFDIEEVVSVYELEGHPDY 615

Query: 605  DYCYGDVVVRLSPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDD 664
            DYC GDVVVRLSPVSDSIEALSSENNT+ELN  SSTNGVMSCTEKVSGSLKIEYPSSSDD
Sbjct: 616  DYCCGDVVVRLSPVSDSIEALSSENNTDELNQPSSTNGVMSCTEKVSGSLKIEYPSSSDD 675

Query: 665  FFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGA 724
             FDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGA
Sbjct: 676  CFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGA 735

Query: 725  ASWETIDDDDDDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVT 784
            ASWET+   DDDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVT
Sbjct: 736  ASWETV---DDDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVT 795

Query: 785  RLATGIFSRGPRNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSD 844
            RLATGIFSRGPRNRESMDLDS SESDTQSLEIQ+LEEIDSGLQSSSLKSNSFTASDMNSD
Sbjct: 796  RLATGIFSRGPRNRESMDLDSRSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTASDMNSD 855

Query: 845  CGRGEDDDASEPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGT 904
            C RGEDDDASEPSK+LVSAEASSNLRTAQSDGS CHEDGTCSFKGFDIAKDPLDHYFLGT
Sbjct: 856  CRRGEDDDASEPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGT 915

Query: 905  NGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFF 964
            NGQTINGRKWLKKIQQDWTILQNNLPDGIYV+VYEDRMDLLR+VIVGAYGTPYQDGLFFF
Sbjct: 916  NGQTINGRKWLKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRSVIVGAYGTPYQDGLFFF 975

Query: 965  DFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQV 1024
            DFHLPP+YPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQV
Sbjct: 976  DFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQV 1035

Query: 1025 LVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFE 1084
            LVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFE
Sbjct: 1036 LVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFE 1095

Query: 1085 ELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKL 1144
            ELVKEHFR+RGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKL
Sbjct: 1096 ELVKEHFRRRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKL 1155

Query: 1145 FSSLNEVRADCRDFKHFQQL 1162
            FSSLNEV ADCRDFKHFQQL
Sbjct: 1156 FSSLNEVGADCRDFKHFQQL 1172

BLAST of CmaCh02G002670 vs. NCBI nr
Match: KAG7027026.1 (putative ubiquitin-conjugating enzyme E2 23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2211.8 bits (5730), Expect = 0.0e+00
Identity = 1114/1150 (96.87%), Postives = 1133/1150 (98.52%), Query Frame = 0

Query: 15   QMGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYR 74
            +MGQQDSVD+ESLTDTCDSYMNRDGVSTSGNIHMPTIN+DEARRMNEN+SDKHN+PHVYR
Sbjct: 14   EMGQQDSVDEESLTDTCDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYR 73

Query: 75   RDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY---GENEGQN 134
            RDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY   GENEGQN
Sbjct: 74   RDIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYDGDGENEGQN 133

Query: 135  KENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAA 194
            KENYDDDTNAKNSNRDSYKNQPL ADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAA
Sbjct: 134  KENYDDDTNAKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAA 193

Query: 195  VSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDI 254
            VSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDF VGDYVVLGPWLGRIDD+
Sbjct: 194  VSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDV 253

Query: 255  LDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLS 314
            LDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLS
Sbjct: 254  LDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLS 313

Query: 315  GLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQL 374
            GLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQL
Sbjct: 314  GLWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQL 373

Query: 375  GDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLD 434
            GDWCLLPSSFSSGSTKD SQTELSDSVTNTLDYAQTVGACDSEDTV+DEL+GRTESTDLD
Sbjct: 374  GDWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLD 433

Query: 435  SISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFE 494
            SISACNGNYRDPEC+ILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKK++NFE
Sbjct: 434  SISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKEENFE 493

Query: 495  RALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTES 554
            RALLIINT+TKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA+DNDDDVTES
Sbjct: 494  RALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTES 553

Query: 555  RRVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 614
            RRVGVVKSVHA ERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR
Sbjct: 554  RRVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVR 613

Query: 615  LSPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWV 674
            LSPVSDSIEALSSENNTEELN QSSTNGVMSC EKVSGSLKIEYPSSSDD FDFSDLSWV
Sbjct: 614  LSPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWV 673

Query: 675  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDD 734
            GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDES GAGSEISNGAASWET+  DD
Sbjct: 674  GNITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESTGAGSEISNGAASWETV--DD 733

Query: 735  DDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRG 794
            DDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRG
Sbjct: 734  DDDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRG 793

Query: 795  PRNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDAS 854
            PRNRESMDLDSHSESDTQSLEIQ+LEEIDSGLQSSSLKSNSFT SDMNSDCGRGEDDDAS
Sbjct: 794  PRNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDAS 853

Query: 855  EPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKW 914
            EPSK+LVSAEASSNLRTAQSDGS CHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKW
Sbjct: 854  EPSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKW 913

Query: 915  LKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPD 974
            LKKIQQDWTILQNNLPDGIYV+VYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPP+YPD
Sbjct: 914  LKKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPD 973

Query: 975  VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN 1034
            VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN
Sbjct: 974  VPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLN 1033

Query: 1035 SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKR 1094
            SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR+R
Sbjct: 1034 SKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRR 1093

Query: 1095 GYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRAD 1154
            GYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEV AD
Sbjct: 1094 GYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGAD 1153

Query: 1155 CRDFKHFQQL 1162
            CRDFKHFQQL
Sbjct: 1154 CRDFKHFQQL 1161

BLAST of CmaCh02G002670 vs. NCBI nr
Match: XP_022947462.1 (probable ubiquitin-conjugating enzyme E2 23 [Cucurbita moschata])

HSP 1 Score: 2209.5 bits (5724), Expect = 0.0e+00
Identity = 1113/1149 (96.87%), Postives = 1131/1149 (98.43%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            MGQQDSVD+ESLTD CDSYMNRDGVSTSGNIHMPTIN+DEARRMNEN+SDKHN+PHVYRR
Sbjct: 1    MGQQDSVDEESLTDACDSYMNRDGVSTSGNIHMPTINSDEARRMNENRSDKHNIPHVYRR 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY---GENEGQNK 135
            DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY   GENEGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDYDGDGENEGQNK 120

Query: 136  ENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 195
            ENYDDDTNAKNSNRDSYKNQPL ADEVRVLWMDESETTQNVNDLTV DRGFLHGDFVAAV
Sbjct: 121  ENYDDDTNAKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVTDRGFLHGDFVAAV 180

Query: 196  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDIL 255
            SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDF VGDYVVLGPWLGRIDD+L
Sbjct: 181  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 256  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 315
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 300

Query: 316  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 375
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 376  DWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDS 435
            DWCLLPSSFSSGSTKD SQTELSDSVTNTLDYAQTVGACDSEDTV+DEL+GRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDLSQTELSDSVTNTLDYAQTVGACDSEDTVVDELSGRTESTDLDS 420

Query: 436  ISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFER 495
            ISACNGNYRDPEC+ILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKK++NFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 496  ALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 555
            ALLIINT+TKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKA+DNDDDVTESR
Sbjct: 481  ALLIINTTTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKATDNDDDVTESR 540

Query: 556  RVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 615
            RVGVVKSVHA ERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 616  SPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVG 675
            SPVSDSIEALSSENNTEELN QSSTNGVMSC EKVSGSLKIEYPSSSDD FDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENNTEELNQQSSTNGVMSCPEKVSGSLKIEYPSSSDDCFDFSDLSWVG 660

Query: 676  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDD 735
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWET+  DDD
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETV--DDD 720

Query: 736  DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 795
            DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP
Sbjct: 721  DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 780

Query: 796  RNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASE 855
            RNRESMDLDSHSESDTQSLEIQ+LEEIDSGLQSSSLKSNSFT SDMNSDCGRGEDDDASE
Sbjct: 781  RNRESMDLDSHSESDTQSLEIQSLEEIDSGLQSSSLKSNSFTTSDMNSDCGRGEDDDASE 840

Query: 856  PSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 915
            PSK+LVSAEASSNLRTAQSDGS CHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL
Sbjct: 841  PSKMLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 900

Query: 916  KKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDV 975
            KKIQQDWTILQNNLPDGIYV+VYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPP+YPDV
Sbjct: 901  KKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960

Query: 976  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1035
            PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS
Sbjct: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1020

Query: 1036 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRG 1095
            KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR+RG
Sbjct: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRG 1080

Query: 1096 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADC 1155
            YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEV ADC
Sbjct: 1081 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1140

Query: 1156 RDFKHFQQL 1162
            RDFKHFQQL
Sbjct: 1141 RDFKHFQQL 1147

BLAST of CmaCh02G002670 vs. NCBI nr
Match: XP_023534561.1 (probable ubiquitin-conjugating enzyme E2 23 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2198.3 bits (5695), Expect = 0.0e+00
Identity = 1107/1149 (96.34%), Postives = 1126/1149 (98.00%), Query Frame = 0

Query: 16   MGQQDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRR 75
            M Q DSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKS+K N+PHVYRR
Sbjct: 1    MAQPDSVDKESLTDTCDSYMNRDGVSTSGNIHMPTINNDEARRMNENKSNKPNIPHVYRR 60

Query: 76   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDY---GENEGQNK 135
            DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDD    GENEGQNK
Sbjct: 61   DIVKSKGSGMIGIVTEVAGDADSDTDITDDEDDYDDGEDGLDDDGCDDSDGDGENEGQNK 120

Query: 136  ENYDDDTNAKNSNRDSYKNQPLTADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 195
            ENYDDDTNAKNSNRDSYKNQPL ADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV
Sbjct: 121  ENYDDDTNAKNSNRDSYKNQPLPADEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAV 180

Query: 196  SNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDIL 255
            SNPTGQ+GVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDF VGDYVVLGPWLGRIDD+L
Sbjct: 181  SNPTGQSGVVVDVNISVDLLVPDGSVVKNISSKDLIRVRDFAVGDYVVLGPWLGRIDDVL 240

Query: 256  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRVRASSSTVFKNSKWLSG 315
            DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQR+RASSSTVFKNSKWLSG
Sbjct: 241  DNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDASFPYYPGQRLRASSSTVFKNSKWLSG 300

Query: 316  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 375
            LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG
Sbjct: 301  LWKPNRLQGTVTKVTVGSVFIYWIASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLG 360

Query: 376  DWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDS 435
            DWCLLPSSFSSGSTKDSSQTEL DSVTNTLDYAQTVGACDSEDTV+DEL GRTESTDLDS
Sbjct: 361  DWCLLPSSFSSGSTKDSSQTELPDSVTNTLDYAQTVGACDSEDTVVDELTGRTESTDLDS 420

Query: 436  ISACNGNYRDPECHILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFER 495
            ISACNGNYRDPEC+ILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKK++NFER
Sbjct: 421  ISACNGNYRDPECNILPESSSSRATKENTHETWPLHRKKIRKVVIRRDKKARKKEENFER 480

Query: 496  ALLIINTSTKVDVVWQDGRTELALDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 555
            ALLIINT+TKVDVVWQDGR EL LDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR
Sbjct: 481  ALLIINTTTKVDVVWQDGRAELVLDSTGLIPINPGDHEFVPEQYVVEKASDNDDDVTESR 540

Query: 556  RVGVVKSVHANERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 615
            RVGVVKSVHA ERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL
Sbjct: 541  RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRL 600

Query: 616  SPVSDSIEALSSENNTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVG 675
            SPVSDSIEALSSEN TEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDD FDFSDLSWVG
Sbjct: 601  SPVSDSIEALSSENTTEELNHQSSTNGVMSCTEKVSGSLKIEYPSSSDDCFDFSDLSWVG 660

Query: 676  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDDD 735
            NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSE+SNGAASWET+  DDD
Sbjct: 661  NITNLKNGDIEVFWANGMVSTVGPQAIYVVGRDDDDESIGAGSEVSNGAASWETV--DDD 720

Query: 736  DDEMDVENAKEDIELQDTGVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGP 795
            DDEMDVENAKEDIELQDTGVDSEEEE+EQHNSGRSLALSVPLAAFRFVTRLATG+FSRGP
Sbjct: 721  DDEMDVENAKEDIELQDTGVDSEEEENEQHNSGRSLALSVPLAAFRFVTRLATGLFSRGP 780

Query: 796  RNRESMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASE 855
            RNR+SMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASD+N DCGRGEDDDASE
Sbjct: 781  RNRDSMDLDSHSESDTQSLEIQALEEIDSGLQSSSLKSNSFTASDLNPDCGRGEDDDASE 840

Query: 856  PSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWL 915
            PSKVLVSAEASSNLRTAQSDGS CHEDGTCSFKGFDI KDPLDHYFLGTNGQTINGRKWL
Sbjct: 841  PSKVLVSAEASSNLRTAQSDGSACHEDGTCSFKGFDIVKDPLDHYFLGTNGQTINGRKWL 900

Query: 916  KKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDV 975
            KKIQQDWTILQNNLPDGIYV+VYEDRMDLLR VIVGAYGTPYQDGLFFFDFHLPP+YPDV
Sbjct: 901  KKIQQDWTILQNNLPDGIYVRVYEDRMDLLRTVIVGAYGTPYQDGLFFFDFHLPPEYPDV 960

Query: 976  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1035
            PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS
Sbjct: 961  PPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNS 1020

Query: 1036 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRG 1095
            KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFR+RG
Sbjct: 1021 KPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRRRG 1080

Query: 1096 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADC 1155
            YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEV ADC
Sbjct: 1081 YYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVGADC 1140

Query: 1156 RDFKHFQQL 1162
            RDFKHFQQL
Sbjct: 1141 RDFKHFQQL 1147

BLAST of CmaCh02G002670 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 684/1131 (60.48%), Postives = 847/1131 (74.89%), Query Frame = 0

Query: 37   RDGVSTSGNIHMPTINNDEARRMNENKSDKHNVPHVYRRDIVKSKGSGMIGIVTEVAGDA 96
            +D   TS N+ + +  +D    ++   S+    P++YR+DIVK+K +G +G+V+EVAGD+
Sbjct: 5    QDDPGTSTNVGVDSSVDDSMASLSICDSEH---PNIYRQDIVKNKKTGSVGVVSEVAGDS 64

Query: 97   DSDTDITDDEDDYDDGEDGLDDDGCDDYGENEGQNKENYDDDTNAKNSNRDSYKNQPLTA 156
            DSD+DI+D+E+D DD ED  DDD  +D  E +  ++EN  +    K ++  +YK   L  
Sbjct: 65   DSDSDISDEEEDDDDDEDNDDDD--EDVEEGKKASEENVVNGDGEKKAD-GNYKCGALEG 124

Query: 157  DEVRVLWMDESETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDG 216
            D++RVLWMD +E  Q++ND+TVIDRGFLHGD+VA+ S PTGQ GVVVDVNISVDLL PDG
Sbjct: 125  DQIRVLWMDNTEPVQDINDVTVIDRGFLHGDYVASASEPTGQVGVVVDVNISVDLLAPDG 184

Query: 217  SVVKNISSKDLIRVRDFTVGDYVVLGPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLK 276
            S+ K+IS+K+L RVRDF VGDYVV GPWLGRIDD+LDNVTV+FDDGS CKV + EP RLK
Sbjct: 185  SIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCKVLRVEPLRLK 244

Query: 277  PVSKNIL-EDASFPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYW 336
            P+ KN L EDA+FPYYPGQRV+ASSS+VFKNS+WLSGLWKPNRL+GTVTKVT GS+F+YW
Sbjct: 245  PIPKNNLEEDANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEGTVTKVTAGSIFVYW 304

Query: 337  IASAGYGPDSSTTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGST---KDSSQT 396
            IASAG+GPDSS +P EEQ+P NL LL+CF+H NWQ+GDWCLLPS   S +    K  S+ 
Sbjct: 305  IASAGFGPDSSVSPPEEQNPSNLTLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKL 364

Query: 397  ELSDSVTNTLDYAQTVGACDSEDTVLDELNGRTESTDLDSISACNGNYRDPECHILPESS 456
             L DS     D  Q +G  D ED V DE++G+ E   + + +       DP     P+ +
Sbjct: 365  RLYDS---QADRQQKIGR-DLED-VQDEVSGKVEPAGITAEALPKVTSDDP-----PQRN 424

Query: 457  SSRATKENTHETWPLHRKKIRKVVIRRDKKARKKDDNFERALLIINTSTKVDVVWQDGRT 516
             S  +KE  HE WPLHRKKIRK+VIR+DKK +KK+++FE+ALL++N+ T+VDV WQDG  
Sbjct: 425  PS-VSKEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALLVVNSRTRVDVSWQDGTI 484

Query: 517  ELALDSTGLIPI-NPGDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTACVRW 576
            E   ++  LIPI  PGDHEFV EQYVVEK SD+ D+ TE RR GVVK+V+A +RTA VRW
Sbjct: 485  ECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRW 544

Query: 577  LKPVSRAEDPREFDIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNTEEL 636
            L P+ RAE+PREF+ EE+VSVYELEGHPDYDYCYGDVVVRLSP++ ++ A S  N+ EE 
Sbjct: 545  LNPLRRAEEPREFEKEEIVSVYELEGHPDYDYCYGDVVVRLSPIAVALPASSPGNSFEEA 604

Query: 637  NHQSSTNGVMSCTEKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLKNGDIEVFWANGMV 696
              Q   NG           + ++    ++   D S LSWVGNIT LK+GDIEV WA+G +
Sbjct: 605  TQQD--NGYQDSESHQEAKILVD-KEENEPSTDLSKLSWVGNITGLKDGDIEVTWADGTI 664

Query: 697  STVGPQAIYVVGRDDDDESIGAGSEISNGAASWETIDDDD-DDDEMDVENAKEDIELQDT 756
            STVGP A+YVVGRDDDDES+   SE S+ AASWET++DDD    E+  E+      ++  
Sbjct: 665  STVGPHAVYVVGRDDDDESVAGESETSD-AASWETLNDDDRGAPEIPEEDLGRSSSIEG- 724

Query: 757  GVDSEEEESEQHNSGRSLALSVPLAAFRFVTRLATGIFSRGPRNRESMDLDSHSESDTQS 816
              +S+ +   +++SGR+ AL++PLAA  FVTRLA+GIFSR    R+S+D  S S+   ++
Sbjct: 725  --NSDADIYAENDSGRNGALALPLAAIEFVTRLASGIFSRA---RKSVD-SSSSDYTVEN 784

Query: 817  LEIQALEEIDSGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSKVLVSAEASSNLRTAQ 876
            +  QA E  +   ++ SL   S +  ++  +C   E       +K ++S E S+ L    
Sbjct: 785  VYKQA-ESTNPSDETDSLDDPSPSKVNVTDNC---ESKGTQANAKNILSGETSTFLE--D 844

Query: 877  SDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQTINGRKWLKKIQQDWTILQNNLPDGI 936
             D     E  +CSF+ FDI++DPLDH+FLG +GQ    R+W KK+ QDW ILQNNLPDGI
Sbjct: 845  EDKPVPSEGDSCSFRRFDISQDPLDHHFLGVDGQKTKERQWFKKVDQDWKILQNNLPDGI 904

Query: 937  YVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLY 996
            +V+ YEDRMDLLRAVIVGA+GTPYQDGLFFFDFHLP DYP VPPSAYYHSGGWR+NPNLY
Sbjct: 905  FVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLY 964

Query: 997  EEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEG 1056
            EEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQ+GTAEG
Sbjct: 965  EEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEG 1024

Query: 1057 EKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGS 1116
            EKNSL YNENTFLLNCKT+MYLMRKPPKDFEEL+K+HFRKRGYYILKACDAYMKG+LIGS
Sbjct: 1025 EKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGS 1084

Query: 1117 LTEDAS-IREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADCRDFKHFQQ 1161
            LT+DAS I E S  NSTSVGFKLMLAKI PKLFS+L+EV ADC +F+H QQ
Sbjct: 1085 LTKDASVIDERSSANSTSVGFKLMLAKIAPKLFSALSEVGADCNEFQHLQQ 1101

BLAST of CmaCh02G002670 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 588.6 bits (1516), Expect = 1.1e-167
Identity = 365/997 (36.61%), Postives = 531/997 (53.26%), Query Frame = 0

Query: 168  ETTQNVNDLTVIDRGFLHGDFVAAVSNPTGQAGVVVDVNISVDLLVPDGSVVKNISSKDL 227
            ET   +++    +RGF++GD V + + P+GQ+G V+++++ V+L    G ++K + +K L
Sbjct: 41   ETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRL 100

Query: 228  IRVRDFTVGDYVVLGPWLGRIDDILDNVTVMFDDGSKCKVTKAEPSRLKPVSKNILEDAS 287
             ++R  ++ DYV+ GPW+GR+D I++ V+V  DDG+  +V      +L  +  N+LED+ 
Sbjct: 101  QKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQ 160

Query: 288  FPYYPGQRVRASSSTVFKNSKWLSGLWKPNRLQGTVTKVTVGSVFIYWIAS-AGYGPDSS 347
            + YYPGQRV+   +   +++ WL G W+  ++ GTV  V  G V++ W+AS    G  + 
Sbjct: 161  YSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNL 220

Query: 348  TTPAEEQSPKNLRLLTCFSHVNWQLGDWCLLPSSFSSGSTKDSSQTELSDSVTNTLDYAQ 407
            T P   Q+P++L LL C SH +WQLGDWC+LP          SS  ++++  T       
Sbjct: 221  TAPQALQNPESLTLLPCVSHASWQLGDWCILPG---------SSHCDIAERQT------- 280

Query: 408  TVGACDSEDTVLDELNGRTESTDLDSISACNGNYRDPECHILPESSSSRATKENTHETWP 467
                                     +++A N N    ECH   +   +R           
Sbjct: 281  ------------------------PNVAAYNLN----ECHKTFQKGFNR----------- 340

Query: 468  LHRKKIRKVVIRRDKKARKKDDNFERALLIINTSTKVDVVWQDGRTELALDSTGLIPINP 527
                               ++   +   +I  T  KV V+WQDG   L +DS  L+P+  
Sbjct: 341  -----------------NMQNSGLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGA 400

Query: 528  -GDHEFVPEQYVVEKASDNDDDVTESRRVGVVKSVHANERTACVRWLKPVSR-AEDPREF 587
               H+F PEQ+VVEK + N      S++ GVVK+V+A E+T  V+W   V + A    + 
Sbjct: 401  VNAHDFWPEQFVVEKETCN------SKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDE 460

Query: 588  DIEEVVSVYELEGHPDYDYCYGDVVVRLSPVSDSIEALSSENNTEELNHQSSTNGVMSCT 647
             +EE+VS YEL  HPD+ +C+ DVVV+L P                        G     
Sbjct: 461  VMEEIVSAYELLEHPDFGFCFSDVVVKLLP-----------------------EGKFDPN 520

Query: 648  EKVSGSLKIEYPSSSDDFFDFSDLSWVGNITNLKNGDIEVFWANGMVSTVGPQAIYVVGR 707
                 + + ++  +  D+     LS +G +T  KNG ++V WANG  S V P  I+ + R
Sbjct: 521  ADTIVATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWANGSTSKVAPCEIWKMER 580

Query: 708  DDDDESIGAGSEISNGAASWETIDDDDDDDEMDVENAKEDIELQDTGVDSEEEESEQHNS 767
             +   S    SE S    S + I   D+      E     ++L   G      ES   N 
Sbjct: 581  SEYSNSSTVSSEGSVQDLS-QKISQSDEASSNHQETGL--VKLYSVG------ESCNENI 640

Query: 768  GRSLALSVPLAAFRFVTRLATGIF----SRGPRNRESMDLDSHSESDTQSLEIQALEEID 827
                +  +P AA  F+T LA+ +F    S    +  S   DS  +SD++ L  +  E  D
Sbjct: 641  PECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQETAESYD 700

Query: 828  SGLQSSSLKSNSFTASDMNSDCGRGEDDDASEPSKVLVSAEASSNLRTAQSDGSTCHED- 887
                              NS+   GE D  +  + V +  E     +T     ST  E+ 
Sbjct: 701  ------------------NSETNSGEVDMTT--TMVNIPIEGKGINKTLD---STLLENS 760

Query: 888  -GTCSFKGFDIAKDPLDHYFLGTN---GQTINGRKWLKKIQQDWTILQNNLPDGIYVQVY 947
                 F+ FD+  D  DH+FL ++    Q+   + W+KK+QQ+W+ L+ NLP+ IYV+V 
Sbjct: 761  RNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVC 820

Query: 948  EDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKV 1007
            E+RMDLLRA +VGA GTPY DGLFFFD  LPP YP  PP  +YHSGG R+NPNLYE G+V
Sbjct: 821  EERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRV 880

Query: 1008 CLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSL 1067
            CLSLLNTW+G G EVW+  SSSILQ+L+S Q LVLN KPYFNEAGYDKQ+G AEGEKNS+
Sbjct: 881  CLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSV 899

Query: 1068 SYNENTFLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDA 1127
            SYNEN FL+ CK+++ ++RKPPK FE LVK+HF  R  ++L AC AYM+G  +GS    A
Sbjct: 941  SYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGS---SA 899

Query: 1128 SIREESDPNSTSVGFKLMLAKIVPKLFSSLNEVRADC 1153
            +++  S  NST  GFK+ML+K+ PKL  + +E+  DC
Sbjct: 1001 NLQGNSTTNST--GFKIMLSKLYPKLLEAFSEIGVDC 899

BLAST of CmaCh02G002670 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 284.6 bits (727), Expect = 3.5e-76
Identity = 135/257 (52.53%), Postives = 176/257 (68.48%), Query Frame = 0

Query: 884  FKGFDIAKDPLDHYFL--GTNGQTINGRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDL 943
            FK FDI +D  DH++   GT  +  + + W K+IQ +W IL+ +LP+ I+V+ YE RMDL
Sbjct: 305  FKRFDIVEDFSDHHYASKGTTSKQ-HSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDL 364

Query: 944  LRAVIVGAYGTPYQDGLFFFDFHLPPDYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLN 1003
            LRAVI+GA GTPY DGLFFFD   P  YP  PP  +YHSGG RINPNLY  GKVCLSLL 
Sbjct: 365  LRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLG 424

Query: 1004 TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENT 1063
            TW+G   E W P +S++LQVLVS+QGL+LN KPYFNE GY++  G+A GE  S +Y+ENT
Sbjct: 425  TWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENT 484

Query: 1064 FLLNCKTIMYLMRKPPKDFEELVKEHFRKRGYYILKACDAYMKGHLIGSLTEDASIREES 1123
            F+L+ KT++Y MR+PPK FE+    HF    + +LKAC+AY  G   G L + A   EE+
Sbjct: 485  FILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEEN 544

Query: 1124 DPNSTSVGFKLMLAKIV 1139
                +S+ F+  +A  V
Sbjct: 545  SAGMSSLKFRTDVATFV 560

BLAST of CmaCh02G002670 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 279.3 bits (713), Expect = 1.5e-74
Identity = 141/291 (48.45%), Postives = 186/291 (63.92%), Query Frame = 0

Query: 849  DASEPSKVLVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGT-NGQTIN 908
            DAS  S   ++AE  S+++ + S+     E+    FK FD  +D  DH++         +
Sbjct: 210  DASASSSHPIAAEPISSVQDS-SNARKLKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQH 269

Query: 909  GRKWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPP 968
             + W+KK+Q DW IL+N+LP+ I V+  E RMDLLRAVI+GA GTPY DGLFFFD   P 
Sbjct: 270  SKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPD 329

Query: 969  DYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQG 1028
             YP VPP+ +YHSGG RINPNLY  GKVCLSLL TW G   E W P  S++LQ+LVS+Q 
Sbjct: 330  TYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQA 389

Query: 1029 LVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKEH 1088
            L+LN KPYFNE GY +  GTA GE  S  Y+EN FLL+ KT++Y +R+PP+ FEE V+ H
Sbjct: 390  LILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNH 449

Query: 1089 FRKRGYYILKACDAYMKGHLIGSLTEDASIREESDPNSTSVGFKLMLAKIV 1139
            +  R + I+KAC+AY  G  +GS+ +      E    S S  FK  +A  +
Sbjct: 450  YFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEARQSGSKKFKTDVASFM 499

BLAST of CmaCh02G002670 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 251.1 bits (640), Expect = 4.2e-66
Identity = 127/265 (47.92%), Postives = 166/265 (62.64%), Query Frame = 0

Query: 857  LVSAEASSNLRTAQSDGSTCHEDGTCSFKGFDIAKDPLDHYFLGTNGQT--------ING 916
            +V  + SSN+R  +       E+    FK FD  +D  DH++                 G
Sbjct: 1    MVEIKDSSNVRKLK-------EEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKG 60

Query: 917  R--KWLKKIQQDWTILQNNLPDGIYVQVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLP 976
            R   W+K IQ++W IL  NLP+ I+V+  E R+DLLRAVI+GA GTPY DGLFFFD   P
Sbjct: 61   RPKNWVKDIQKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFP 120

Query: 977  PDYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQ 1036
              YP VPP  +YHSGG RINPNLY+ GKVCLSL++TWTG+  E W PK S++LQ+LVS+Q
Sbjct: 121  DTYPSVPPKVHYHSGGLRINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQ 180

Query: 1037 GLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTIMYLMRKPPKDFEELVKE 1096
             L+LN KPY+NE GY+K +GT  GE  S  Y+EN F+ + KT+          FEE V+ 
Sbjct: 181  ALILNEKPYYNEPGYEKSMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRS 240

Query: 1097 HFRKRGYYILKACDAYMKGHLIGSL 1112
            HF  R + I+KAC+AY  G  +GS+
Sbjct: 241  HFFVRSHDIVKACNAYKDGAPVGSI 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVX10.0e+0060.48Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q8VY101.6e-16636.61Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q9C0C91.8e-7725.59(E3-independent) E2 ubiquitin-conjugating enzyme OS=Homo sapiens OX=9606 GN=UBE2... [more]
Q6ZPJ35.7e-7624.96(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O OS=Mus musculus OX=10090 ... [more]
Q9LUQ54.9e-7552.53Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Match NameE-valueIdentityDescription
A0A6J1I4760.0e+00100.00probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1G6I70.0e+0096.87probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JD250.0e+0087.22probable ubiquitin-conjugating enzyme E2 23 isoform X2 OS=Cucurbita maxima OX=36... [more]
A0A6J1JF340.0e+0086.47probable ubiquitin-conjugating enzyme E2 23 isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1FVD70.0e+0086.72probable ubiquitin-conjugating enzyme E2 23 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022970910.10.0e+00100.00probable ubiquitin-conjugating enzyme E2 23 [Cucurbita maxima][more]
KAG6604947.10.0e+0096.47putative ubiquitin-conjugating enzyme E2 23, partial [Cucurbita argyrosperma sub... [more]
KAG7027026.10.0e+0096.87putative ubiquitin-conjugating enzyme E2 23, partial [Cucurbita argyrosperma sub... [more]
XP_022947462.10.0e+0096.87probable ubiquitin-conjugating enzyme E2 23 [Cucurbita moschata][more]
XP_023534561.10.0e+0096.34probable ubiquitin-conjugating enzyme E2 23 isoform X2 [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
AT2G16920.10.0e+0060.48ubiquitin-conjugating enzyme 23 [more]
AT2G33770.11.1e-16736.61phosphate 2 [more]
AT3G15355.13.5e-7652.53ubiquitin-conjugating enzyme 25 [more]
AT1G53025.11.5e-7448.45Ubiquitin-conjugating enzyme family protein [more]
AT1G53023.14.2e-6647.92Ubiquitin-conjugating enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 738..758
NoneNo IPR availableSMARTSM00212ubc_7coord: 913..1070
e-value: 3.6E-22
score: 89.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 857..877
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 822..877
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 710..767
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 725..742
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 822..839
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..50
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 790..809
NoneNo IPR availablePANTHERPTHR46116:SF21UBIQUITIN-CONJUGATING ENZYME E2 23-RELATEDcoord: 59..1160
NoneNo IPR availablePANTHERPTHR46116(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYMEcoord: 59..1160
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 915..1059
e-value: 9.2E-23
score: 80.4
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 910..1093
score: 33.99464
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 913..1063
e-value: 3.02176E-32
score: 120.385
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 879..1146
e-value: 1.2E-78
score: 266.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 896..1077

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G002670.1CmaCh02G002670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061631 ubiquitin conjugating enzyme activity